Polynucleotides and polypeptides in plants

ABSTRACT

The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.

RELATIONSHIP TO COPENDING APPLICATIONS

This application claims the benefit of U.S. Non-provisional application Ser. No. 09/837,944, filed Apr. 18, 2001 (abandoned); U.S. Provisional Application No. 60/310,847, filed Aug. 9, 2001 (expired); U.S. Nonprovisional application Ser. No. 09/934,455 (abandoned), filed Aug. 22, 2001; U.S. Provisional Application No. 60/336,049, filed Nov. 19, 2001 (expired); U.S. Provisional Application No. 60/338,692, filed Dec. 11, 2001 (expired); U.S. Non-provisional application Ser. No. 10/171,468, filed Jun. 14, 2002 (abandoned); U.S. Non-provisional application Ser. No. 10/225,066, filed Aug. 9, 2002, which issued as U.S. Pat. No. 7,238,860 on Jul. 3, 2007; U.S. Non-provisional application Ser. No. 10/225,067, filed Aug. 9, 2002, which issued as U.S. Pat. No. 7,135,616, which filed on Nov. 14, 2006; and U.S. Non-provisional application Ser. No. 10/225,068, filed Aug. 9, 2002, which issued as U.S. Pat. No. 7,193,129 on Mar. 20, 2007; the contents of which are hereby incorporated by reference in their entirety.

The claimed invention, in the field of functional genomics and the characterization of plant genes for the improvement of plants, was made by or on behalf of Mendel Biotechnology, Inc. and Monsanto Company as a result of activities undertaken within the scope of a joint research agreement, said agreement having been in effect on or before the date the claimed invention was made.

TECHNICAL FIELD

This invention relates to the field of plant biology. More particularly, the present invention pertains to compositions and methods for modifying a plant phenotypically.

BACKGROUND OF THE INVENTION

A plant's traits, such as its biochemical, developmental, or phenotypic characteristics, may be controlled through a number of cellular processes. One important way to manipulate that control is through transcription factors—proteins that influence the expression of a particular gene or sets of genes. Transformed and transgenic plants that comprise cells having altered levels of at least one selected transcription factor, for example, possess advantageous or desirable traits. Strategies for manipulating traits by altering a plant cell's transcription factor content can therefore result in plants and crops with new and/or improved commercially valuable properties.

Transcription factors can modulate gene expression, either increasing or decreasing (inducing or repressing) the rate of transcription. This modulation results in differential levels of gene expression at various developmental stages, in different tissues and cell types, and in response to different exogenous (e.g., environmental) and endogenous stimuli throughout the life cycle of the organism.

Because transcription factors are key controlling elements of biological pathways, altering the expression levels of one or more transcription factors can change entire biological pathways in an organism. For example, manipulation of the levels of selected transcription factors may result in increased expression of economically useful proteins or biomolecules in plants or improvement in other agriculturally relevant characteristics. Conversely, blocked or reduced expression of a transcription factor may reduce biosynthesis of unwanted compounds or remove an undesirable trait. Therefore, manipulating transcription factor levels in a plant offers tremendous potential in agricultural biotechnology for modifying a plant's traits. A number of the agriculturally relevant characteristics of plants, and desirable traits that may be imbued by gene expression are listed below.

Useful Plant Traits

Category: Abiotic Stress; Desired Trait: Chilling Tolerance

The term “chilling sensitivity” has been used to describe many types of physiological damage produced at low, but above freezing, temperatures. Most crops of tropical origins such as soybean, rice, maize and cotton are easily damaged by chilling. Typical chilling damage includes wilting, necrosis, chlorosis or leakage of ions from cell membranes. The underlying mechanisms of chilling sensitivity are not completely understood yet, but probably involve the level of membrane saturation and other physiological deficiencies. For example, photoinhibition of photosynthesis (disruption of photosynthesis due to high light intensities) often occurs under clear atmospheric conditions subsequent to cold late summer/autumn nights. By some estimates, chilling accounts for monetary losses in the United States (US) second only to drought and flooding. For example, chilling may lead to yield losses and lower product quality through the delayed ripening of maize. Another consequence of poor growth is the rather poor ground cover of maize fields in spring, often resulting in soil erosion, increased occurrence of weeds, and reduced uptake of nutrients. A retarded uptake of mineral nitrogen could also lead to increased losses of nitrate into the ground water.

Category: Abiotic Stress; Desired Trait: Freezing Tolerance.

Freezing is a major environmental stress that limits where crops can be grown and reduces yields considerably, depending on the weather in a particular growing season. In addition to exceptionally stressful years that cause measurable losses of billions of dollars, less extreme stress almost certainly causes smaller yield reductions over larger areas to produce yield reductions of similar dollar value every year. For instance, in the US, the 1995 early fall frosts are estimated to have caused losses of over one billion dollars to corn and soybeans. The spring of 1998 saw an estimated $200 M of damages to Georgia alone, in the peach, blueberry and strawberry industries. The occasional freezes in Florida have shifted the citrus belt further south due to $100 M or more losses. California sustained $650 M of damage in 1998 to the citrus crop due to a winter freeze. In addition, certain crops such as Eucalyptus, which has the very favorable properties of rapid growth and good wood quality for pulping, are not able to grow in the southeastern states due to occasional freezes.

Inherent winter hardiness of the crop determines in which agricultural areas it can survive the winter. For example, for wheat, the northern central portion of the US has winters that are too cold for good winter wheat crops. Approximately 20% of the US wheat crop is spring wheat, with a market value of $2 billion. Areas growing spring wheat could benefit by growing winter wheat that had increased winter hardiness. Assuming a 25% yield increase when growing winter wheat, this would create $500 M of increased value. Additionally, the existing winter wheat is severely stressed by freezing conditions and should have improved yields with increased tolerance to these stresses. An estimate of the yield benefit of these traits is 10% of the $4.4 billion winter wheat crop in the US or $444 M of yield increase, as well as better survival in extreme freezing conditions that occur periodically.

Thus plants more resistant to freezing, both midwinter freezing and sudden freezes, would protect a farmers' investment, improve yield and quality, and allow some geographies to grow more profitable and productive crops. Additionally, winter crops such as canola, wheat and barley have 25% to 50% yield increases relative to spring planted varieties of the same crops. This yield increase is due to the “head start” the fall planted crop has over the spring planted crop and its reaching maturity earlier while the temperatures, soil moisture and lack of pathogens provide more favorable conditions.

Category: Abiotic Stress; Desired Trait: Salt Tolerance.

One in five hectares of irrigated land is damaged by salt, an important historical factor in the decline of ancient agrarian societies. This condition is only expected to worsen, further reducing the availability of arable land and crop production, since none of the top five food crops—wheat, corn, rice, potatoes, and soybean—can tolerate excessive salt.

Detrimental effects of salt on plants are a consequence of both water deficit resulting in osmotic stress (similar to drought stress) and the effects of excess sodium ions on critical biochemical processes. As with freezing and drought, high saline causes water deficit; the presence of high salt makes it difficult for plant roots to extract water from their environment (Buchanan et al. (2000) in Biochemistry and Molecular Biology of Plants, American Society of Plant Physiologists, Rockville, Md.). Soil salinity is thus one of the more important variables that determines where a plant may thrive. In many parts of the world, sizable land areas are uncultivable due to naturally high soil salinity. To compound the problem, salination of soils that are used for agricultural production is a significant and increasing problem in regions that rely heavily on agriculture. The latter is compounded by over-utilization, over-fertilization and water shortage, typically caused by climatic change and the demands of increasing population. Salt tolerance is of particular importance early in a plant's lifecycle, since evaporation from the soil surface causes upward water movement, and salt accumulates in the upper soil layer where the seeds are placed. Thus, germination normally takes place at a salt concentration much higher than the mean salt level in the whole soil profile.

Category: Abiotic Stress; Desired Trait: Drought Tolerance.

While much of the weather that we experience is brief and short-lived, drought is a more gradual phenomenon, slowly taking hold of an area and tightening its grip with time. In severe cases, drought can last for many years, and can have devastating effects on agriculture and water supplies. With burgeoning population and chronic shortage of available fresh water, drought is not only the number one weather related problem in agriculture, it also ranks as one of the major natural disasters of all time, causing not only economic damage, but also loss of human lives. For example, losses from the US drought of 1988 exceeded $40 billion, exceeding the losses caused by Hurricane Andrew in 1992, the Mississippi River floods of 1993, and the San Francisco earthquake in 1989. In some areas of the world, the effects of drought can be far more severe. In the Horn of Africa the 1984-1985 drought led to a famine that killed 750,000 people.

Problems for plants caused by low water availability include mechanical stresses caused by the withdrawal of cellular water. Drought also causes plants to become more susceptible to various diseases (Simpson (1981). “The Value of Physiological Knowledge of Water Stress in Plants”, In Water Stress on Plants, (Simpson, G. M., ed.), Praeger, N.Y., pp. 235-265).

In addition to the many land regions of the world that are too arid for most if not all crop plants, overuse and over-utilization of available water is resulting in an increasing loss of agriculturally-usable land, a process which, in the extreme, results in desertification. The problem is further compounded by increasing salt accumulation in soils, as described above, which adds to the loss of available water in soils.

Category: Abiotic Stress; Desired Trait: Heat Tolerance.

Germination of many crops is very sensitive to temperature. A transcription factor that would enhance germination in hot conditions would be useful for crops that are planted late in the season or in hot climates.

Seedlings and mature plants that are exposed to excess heat may experience heat shock, which may arise in various organs, including leaves and particularly fruit, when transpiration is insufficient to overcome heat stress. Heat also damages cellular structures, including organelles and cytoskeleton, and impairs membrane function (Buchanan, supra).

Heat shock may result a decrease in overall protein synthesis, accompanied by expression of heat shock proteins. Heat shock proteins function as chaperones and are involved in refolding proteins denatured by heat.

Category: Abiotic Stress; Desired Trait: Tolerance to Low Nitrogen and Phosphorus.

The ability of all plants to remove nutrients from their environment is essential to survival. Thus, identification of genes that encode polypeptides with transcription factor activity may allow for the generation of transgenic plants that are better able to make use of available nutrients in nutrient-poor environments.

Among the most important macronutrients for plant growth that have the largest impact on crop yield are nitrogenous and phosphorus-containing compounds. Nitrogen- and phosphorus-containing fertilizers are used intensively in agriculture practices today. An increase in grain crop yields from 0.5 to 1.0 metric tons per hectare to 7 metric tons per hectare accompanied the use of commercial fixed nitrogen fertilizer in production farming (Vance (2001) Plant Physiol. 127: 390-397). Given current practices, in order to meet food production demands in years to come, considerable increases in the amount of nitrogen- and phosphorus-containing fertilizers will be required (Vance, supra).

Nitrogen is the most abundant element in the Earth's atmosphere yet it is one of the most limiting elements to plant growth due to its lack of availability in the soil. Plants obtain N from the soil from several sources including commercial fertilizers, manure and the mineralization of organic matter. The intensive use of N fertilizers in present agricultural practices is problematic, the energy intensive Haber-Bosch process makes N fertilizer and it is estimated that the US uses annually between 3-5% of the nation's natural gas for this process. In addition to the expense of N fertilizer production and the depletion of non-renewable resources, the use of N fertilizers has led to the eutrophication of freshwater ecosystems and the contamination of drinking water due to the runoff of excess fertilizer into ground water supplies.

Phosphorus is second only to N in its importance as a macronutrient for plant growth and to its impact on crop yield. Phosphorus (P) is extremely immobile and not readily available to roots in the soil and is therefore often growth limiting to plants. Inorganic phosphate (Pi) is a constituent of several important molecules required for energy transfer, metabolic regulation and protein activation (Marschner (1995) Mineral Nutrition of Higher Plants, 2nd ed., Academic Press, San Diego, Calif.). Plants have evolved several strategies to help cope with P and N deprivation that include metabolic as well as developmental adaptations. Most, if not all, of these strategies have components that are regulated at the level of transcription and therefore are amenable to manipulation by transcription factors. Metabolic adaptations include increasing the availability of P and N by increasing uptake from the soil though the induction of high affinity and low affinity transporters, and/or increasing its mobilization in the plant. Developmental adaptations include increases in primary and secondary roots, increases in root hair number and length, and associations with mycorrhizal fungi (Bates and Lynch (1996) Plant Cell Environ. 19: 529-538; Harrison (1999) Annu. Rev. Plant Physiol. Plant Mol. Biol. 50: 361-389).

Category: Biotic Stress; Desired Trait: Disease Resistance.

Disease management is a significant expense in crop production worldwide. According to EPA reports for 1996 and 1997, US farmers spend approximately $6 billion on fungicides annually. Despite this expenditure, according to a survey conducted by the food and agriculture organization, plant diseases still reduce worldwide crop productivity by 12% and in the United States alone, economic losses due to plant pathogens amounts to 9.1 billion dollars (FAO, 1993). Data from these reports and others demonstrate that despite the availability of chemical control only a small proportion of the losses due to disease can be prevented. Not only are fungicides and anti-bacterial treatments expensive to growers, but their widespread application poses both environmental and health risks. The use of plant biotechnology to engineer disease resistant crops has the potential to make a significant economic impact on agriculture and forestry industries in two ways: reducing the monetary and environmental expense of fungicide application and reducing both pre-harvest and post-harvest crop losses that occur now despite the use of costly disease management practices.

Fungal, bacterial, oomycete, viral, and nematode diseases of plants are ubiquitous and important problems, and often severely impact yield and quality of crop and other plants. A very few examples of diseases of plants include:

Powdery mildew, caused by the fungi Erysiphe, Sphaerotheca, Phyllactinia, Microsphaera, Podosphaera, or Uncinula, in, for example, wheat, bean, cucurbit, lettuce, pea, grape, tree fruit crops, as well as roses, phlox, lilacs, grasses, and Euonymus;

Fusarium-caused diseases such as Fusarium wilt in cucurbits, Fusarium head blight in barley and wheat, wilt and crown and root rot in tomatoes;

Sudden oak death, caused by the oomycete Phytophthora ramorum; this disease was first detected in 1995 in California tan oaks. The disease has since killed more than 100,000 tan oaks, coast live oaks, black oaks, and Shreve's oaks in coastal regions of northern California, and more recently in southwestern Oregon (Roach (2001) National Geographic News, Dec. 6, 2001);

Black Sigatoka, a fungal disease caused by Mycosphaerella species that attacks banana foliage, is spreading throughout the regions of the world that are responsible for producing most of the world's banana crop;

Eutypa dieback, caused by Eutypa lata, affects a number of crop plants, including vine grape. Eutypa dieback delays shoot emergence, and causes chlorosis, stunting, and tattering of leaves;

Pierce's disease, caused by the bacterium Xylella fastidiosa, precludes growth of grapes in the southeastern United States, and threatens the profitable wine grape industry in northern California. The bacterium clogs the vasculature of the grapevines, resulting in foliar scorching followed by slow death of the vines. There is no known treatment for Pierce's disease;

Bacterial Spot caused by the bacterium Xanthomonas campestris causes serious disease problems on tomatoes and peppers. It is a significant problem in the Florida tomato industry because it spreads rapidly, especially in warm periods where there is wind-driven rain. Under these conditions, there are no adequate control measures;

Diseases caused by viruses of the family Geminiviridae are a growing agricultural problem worldwide. Geminiviruses have caused severe crop losses in tomato, cassava, and cotton. For instance, in the 1991-1992 growing season in Florida, geminiviruses caused $140 million in damages to the tomato crop (Moffat (1991) Science 286: 1835). Geminiviruses have the ability to recombine between strains to rapidly produce new virulent varieties. Therefore, there is a pressing need for broad-spectrum geminivirus control;

The soybean cyst nematode, Heterodera glycines, causes stunting and chlorosis of soybean plants, which results in yield losses or plant death from severe infestation. Annual losses in the United States have been estimated at $1.5 billion (University of Minnesota Extension Service).

The aforementioned pathogens represent a very small fraction of diverse species that seriously affect plant health and yield. For a more complete description of numerous plant diseases, see, for example, Vidhyasekaran (1997) Fungal Pathogenesis in Plants and Crops: Molecular Biology and Host Defense Mechanisms, Marcel Dekker, Monticello, N.Y.), or Agrios (1997) Plant Pathology, Academic Press, New York, N.Y.). Plants that are able to resist disease may produce significantly higher yields and improved food quality. It is thus of considerable importance to find genes that reduce or prevent disease.

Category: Light Response; Desired Trait: Reduced Shade Avoidance.

Shade avoidance describes the process in which plants grown in close proximity attempt to out-compete each other by increasing stem length at the expense of leaf, fruit and storage organ development. This is caused by the plant's response to far-red radiation reflected from leaves of neighboring plants, which is mediated by phytochrome photoreceptors. Close proximity to other plants, as is produced in high-density crop plantings, increases the relative proportion of far-red irradiation, and therefore induces the shade avoidance response. Shade avoidance adversely affects biomass and yield, particularly when leaves, fruits or other storage organs constitute the desired crop (see, for example, Smith (1982) Annu. Rev. Plant Physiol. 33: 481-518; Ballare et al. (1990) Science 247: 329-332; Smith (1995) Annu. Dev. Plant Physiol. Mol. Biol., 46: 289-315; and Schmitt et al. (1995), American Naturalist, 146: 937-953). Alteration of the shade avoidance response in tobacco through alteration of phytochrome levels has been shown to produce an increase in harvest index (leaf biomass/total biomass) at high planting density, which would result in higher yield (Robson et al. (1996) Nature Biotechnol. 14: 995-998).

Category: Flowering Time; Desired Trait: Altered Flowering Time and Flowering Control.

Timing of flowering has a significant impact on production of agricultural products. For example, varieties with different flowering responses to environmental cues are necessary to adapt crops to different production regions or systems. Such a range of varieties have been developed for many crops, including wheat, corn, soybean, and strawberry. Improved methods for alteration of flowering time will facilitate the development of new, geographically adapted varieties.

Breeding programs for the development of new varieties can be limited by the seed-to-seed cycle. Thus, breeding new varieties of plants with multi-year cycles (such as biennials, e.g. carrot, or fruit trees, such as citrus) can be very slow. With respect to breeding programs, there would be a significant advantage in having commercially valuable plants that exhibit controllable and modified periods to flowering (“flowering times”). For example, accelerated flowering would shorten crop and tree breeding programs.

Improved flowering control allows more than one planting and harvest of a crop to be made within a single season. Early flowering would also improve the time to harvest plants in which the flower portion of the plant constitutes the product (e.g., broccoli, cauliflower, and other edible flowers). In addition, chemical control of flowering through induction or inhibition of flowering in plants could provide a significant advantage to growers by inducing more uniform fruit production (e.g., in strawberry)

A sizable number of plants for which the vegetative portion of the plant forms the valuable crop tend to “bolt” dramatically (e.g., spinach, onions, lettuce), after which biomass production declines and product quality diminishes (e.g., through flowering-triggered senescence of vegetative parts). Delay or prevention of flowering may also reduce or preclude dissemination of pollen from transgenic plants.

Category: Growth Rate; Desired Trait: Modified Growth Rate.

For almost all commercial crops, it is desirable to use plants that establish more quickly, since seedlings and young plants are particularly susceptible to stress conditions such as salinity or disease. Since many weeds may outgrow young crops or out-compete them for nutrients, it would also be desirable to determine means for allowing young crop plants to out compete weed species. Increasing seedling growth rate (emergence) contributes to seedling vigor and allows for crops to be planted earlier in the season with less concern for losses due to environmental factors. Early planting helps add days to the critical grain-filling period and increases yield.

Providing means to speed up or slow down plant growth would also be desirable to ornamental horticulture. If such means be provided, slow growing plants may exhibit prolonged pollen-producing or fruiting period, thus improving fertilization or extending harvesting season.

Category: Growth Rate; Desired Trait: Modified Senescence and Cell Death.

Premature senescence, triggered by various plant stresses, can limit production of both leaf biomass and seed yield. Transcription factor genes that suppress premature senescence or cell death in response to stresses can provide means for increasing yield. Delay of normal developmental senescence could also enhance yield, particularly for those plants for which the vegetative part of the plant represents the commercial product (e.g., spinach, lettuce).

Although leaf senescence is thought to be an evolutionary adaptation to recycle nutrients, the ability to control senescence in an agricultural setting has significant value. For example, a delay in leaf senescence in some maize hybrids is associated with a significant increase in yields and a delay of a few days in the senescence of soybean plants can have a large impact on yield. In an experimental setting, tobacco plants engineered to inhibit leaf senescence had a longer photosynthetic lifespan, and produced a 50% increase in dry weight and seed yield (Gan and Amasino (1995) Science 270: 1986-1988). Delayed flower senescence may generate plants that retain their blossoms longer and this may be of potential interest to the ornamental horticulture industry, and delayed foliar and fruit senescence could improve post-harvest shelf-life of produce.

Further, programmed cell death plays a role in other plant responses, including the resistance response to disease, and some symptoms of diseases, for example, as caused by necrotrophic pathogens such as Botrytis cinerea and Sclerotinia sclerotiorum (Dickman et al. Proc. Natl. Acad. Sci., 98: 6957-6962). Localized senescence and/or cell death can be used by plants to contain the spread of harmful microorganisms. A specific localized cell death response, the “hypersensitive response”, is a component of race-specific disease resistance mediated by plant resistance genes. The hypersensitive response is thought to help limit pathogen growth and to initiate a signal transduction pathway that leads to the induction of systemic plant defenses. Accelerated senescence may be a defense against obligate pathogens, such as powdery mildew, that rely on healthy plant tissue for nutrients. With regard to powdery mildew, Botrytis cinerea and Sclerotinia sclerotiorum and other pathogens, transcription factors that ameliorate cell death and/or damage may reduce the significant economic losses encountered, such as, for example, Botrytis cinerea in strawberry and grape.

Category: Growth Regulator; Desired Trait: Altered Sugar Sensing

Sugars are key regulatory molecules that affect diverse processes in higher plants including germination, growth, flowering, senescence, sugar metabolism and photosynthesis. Sucrose, for example, is the major transport form of photosynthate and its flux through cells has been shown to affect gene expression and alter storage compound accumulation in seeds (source-sink relationships). Glucose-specific hexose-sensing has also been described in plants and is implicated in cell division and repression of “famine” genes (photosynthetic or glyoxylate cycles).

Category: Morphology; Desired Trait: Altered Morphology

Trichomes are branched or unbranched epidermal outgrowths or hair structures on a plant. Trichomes produce a variety of secondary biochemicals such as diterpenes and waxes, the former being important as, for example, insect pheromones, and the latter as protectants against desiccation and herbivorous pests. Since diterpenes also have commercial value as flavors, aromas, pesticides and cosmetics, and potential value as anti-tumor agents and inflammation-mediating substances, they have been both products and the target of considerable research. In most cases where the metabolic pathways are impossible to engineer, increasing trichome density or size on leaves may be the only way to increase plant productivity. Thus, it would be advantageous to discover trichome-affecting transcription factor genes for the purpose of increasing trichome density, size, or type to produce plants that are better protected from insects or that yield higher amounts of secondary metabolites.

The ability to manipulate wax composition, amount, or distribution could modify plant tolerance to drought and low humidity or resistance to insects, as well as plant appearance. In particular, a possible application for a transcription factor gene that reduces wax production in sunflower seed coats would be to reduce fouling during seed oil processing. Antisense or co-suppression of transcription factors involved in wax biosynthesis in a tissue specific manner can be used to specifically alter wax composition, amount, or distribution in those plants and crops from which wax is either a valuable attribute or product or an undesirable constituent of plants.

Other morphological characteristics that may be desirable in plants include those of an ornamental nature. These include changes in seed color, overall color, leaf and flower shape, leaf color, leaf size, or glossiness of leaves. Plants that produce dark leaves may have benefits for human health; flavonoids, for example, have been used to inhibit tumor growth, prevent of bone loss, and prevention lipid oxidation in animals and humans. Plants in which leaf size is increased would likely provide greater biomass, which would be particularly valuable for crops in which the vegetative portion of the plant constitutes the product. Plants with glossy leaves generally produce greater epidermal wax, which, if it could be augmented, resulted in a pleasing appearance for many ornamentals, help prevent desiccation, and resist herbivorous insects and disease-causing agents. Changes in plant or plant part coloration, brought about by modifying, for example, anthocyanin levels, would provide novel morphological features.

In many instances, the seeds of a plant constitute a valuable crop. These include, for example, the seeds of many legumes, nuts and grains. The discovery of means for producing larger seed would provide significant value by bringing about an increase in crop yield.

Plants with altered inflorescence, including, for example, larger flowers or distinctive floral configurations, may have high value in the ornamental horticulture industry.

Modifications to flower structure may have advantageous or deleterious effects on fertility, and could be used, for example, to decrease fertility by the absence, reduction or screening of reproductive components. This could be a desirable trait, as it could be exploited to prevent or minimize the escape of the pollen of genetically modified organisms into the environment.

Manipulation of inflorescence branching patterns may also be used to influence yield and offer the potential for more effective harvesting techniques. For example, a “self pruning” mutation of tomato results in a determinate growth pattern and facilitates mechanical harvesting (Pnueli et al. (2001) Plant Cell 13(12): 2687-2702).

Alterations of apical dominance or plant architecture could create new plant varieties. Dwarf plants may be of potential interest to the ornamental horticulture industry.

Category: Seed Biochemistry; Desired Trait: Altered Seed Oil

The composition of seeds, particularly with respect to seed oil quantity and/or composition, is very important for the nutritional value and production of various food and feed products. Desirable improvements to oils include enhanced heat stability, improved nutritional quality through, for example, reducing the number of calories in seed, increasing the number of calories in animal feeds, or altering the ratio of saturated to unsaturated lipids comprising the oils.

Category: Seed Biochemistry; Desired Trait: Altered Seed Protein

As with seed oils, seed protein content and composition is very important for the nutritional value and production of various food and feed products. Altered protein content or concentration in seeds may be used to provide nutritional benefits, and may also prolong storage capacity, increase seed pest or disease resistance, or modify germination rates. Altered amino acid composition of seeds, through altered protein composition, is also a desired objective for nutritional improvement.

Category: Seed Biochemistry; Desired Trait: Altered Prenyl Lipids.

Prenyl lipids, including the tocopherols, play a role in anchoring proteins in membranes or membranous organelles. Tocopherols have both anti-oxidant and vitamin E activity. Modified tocopherol composition of plants may thus be useful in improving membrane integrity and function, which may mitigate abiotic stresses such as heat stress. Increasing the anti-oxidant and vitamin content of plants through increased tocopherol content can provide useful human health benefits.

Category: Leaf Biochemistry; Desired Trait: Altered Glucosinolate Levels

Increases or decreases in specific glucosinolates or total glucosinolate content can be desirable depending upon the particular application. For example: (i) glucosinolates are undesirable components of the oilseeds used in animal feed, since they produce toxic effects; low-glucosinolate varieties of canola have been developed to combat this problem; (ii) some glucosinolates have anti-cancer activity; thus, increasing the levels or composition of these compounds can be of use in production of nutraceuticals; and (iii) glucosinolates form part of a plant's natural defense against insects; modification of glucosinolate composition or quantity could therefore afford increased protection from herbivores. Furthermore, tissue specific promoters can be used in edible crops to ensure that these compounds accumulate specifically in particular tissues, such as the epidermis, which are not taken for human consumption.

Category: Leaf Biochemistry; Desired Trait: Flavonoid Production.

Expression of transcription factors that increase flavonoid production in plants, including anthocyanins and condensed tannins, may be used to alter pigment production for horticultural purposes, and possibly to increase stress resistance. Flavonoids have antimicrobial activity and could be used to engineer pathogen resistance. Several flavonoid compounds have human health promoting effects such as inhibition of tumor growth, prevention of bone loss and prevention of lipid oxidation. Increased levels of condensed tannins in forage legumes would provide agronomic benefits in ruminants by preventing pasture bloat by collapsing protein foams within the rumen. For a review on the utilities of flavonoids and their derivatives, see Dixon et al. (1999) Trends Plant Sci. 4: 394-400.

The present invention relates to methods and compositions for producing transgenic plants with modified traits, particularly traits that address the agricultural and food needs described in the above background information. These traits may provide significant value in that they allow the plant to thrive in hostile environments, where, for example, temperature, water and nutrient availability or salinity may limit or prevent growth of non-transgenic plants. The traits may also comprise desirable morphological alterations, larger or smaller size, disease and pest resistance, alterations in flowering time, light response, and others.

We have identified polynucleotides encoding transcription factors, developed numerous transgenic plants using these polynucleotides, and have analyzed the plants for a variety of important traits. In so doing, we have identified important polynucleotide and polypeptide sequences for producing commercially valuable plants and crops as well as the methods for making them and using them. Other aspects and embodiments of the invention are described below and can be derived from the teachings of this disclosure as a whole.

SUMMARY OF THE INVENTION

Transgenic plants and methods for producing transgenic plants are provided. The transgenic plants comprise a recombinant polynucleotide having a polynucleotide sequence, or a sequence that is complementary to this polynucleotide sequence, that encodes a transcription factor.

The polynucleotide sequences that encode the transcription factors are listed in the Sequence Listing and include any of any of SEQ ID NO: 2N−1, wherein N=1-229, SEQ ID NO: 459-466; 468-487; 491-500; 504; 506-511; 516-520; 523-524; 527; 529; 531-533; 538-539; 541-557; 560-568; 570-586; 595-596; 598-606; 610-620; 627-634; 640-664; 670-707; 714-719; 722-735; 740-741; 743-779; 808-823; 825-834; 838-850; 855-864; 868-889; 892-902; 908-909; 914-921; 924-925; 927-932; 935-942; 944-952; 961-965; 968-986; 989-993; 995-1010; 1012-1034; 1043-1063; 1074-1080; 1091-1104; 1111-1121; 1123-1128; 1134-1138; 1142-1156; 1159-1175; 1187-1190; 1192-1199; 1202-1220; 1249-1253; 1258-1262; 1264-1269; 1271-1287; 1292-1301; 1303-1309; 1315-1323; 1328-1337; 1340-1341; 1344-1361; 1365-1377; 1379-1390; 1393-1394; 1396-1398; 1419-1432; 1434-1452; 1455-1456; 1460-1465; 1468-1491; 1499; 1502; 1505-1521; 1523-1527; 1529-1532; 1536-1539; 1542-1562; 1567-1571; 1573-1582; 1587-1592; 1595-1620; 1625-1644; 1647-1654; 1659-1669; 1671-1673; 1675-1680; 1682-1686; 1688-1700; 1706-1709; 1714-1726; 1728-1734; 1738-1742; 1744-1753; 1757-1760; 1763-1764; 1766-1768; 1770-1780; 1782-1784; 1786-1789; 1791-1804; 1806-1812; 1814-1837; 1847-1856; 1858-1862; 1864-1873; 1876-1882; 1885-1896; 1902-1910; 1913-1916; 1921-1928; 1931-1936; 1940-1941; 1944-1946, or SEQ ID NO: 2N−1, wherein N=974-1101.

The transcription factors are comprised of polypeptide sequences listed in the Sequence Listing and include any of SEQ ID NO: 2N, wherein N=1-229, SEQ ID NO: 467; 488-490; 501-503; 505; 512-515; 521-522; 525-526; 528; 530; 534-537; 540; 558-559; 569; 587-594; 597; 607-609; 621-626; 635-639; 665-669; 708-713; 720-721; 736-739; 742; 780-807; 824; 835-837; 851-854; 865-867; 890-891; 903-907; 910-913; 922-923; 926; 933-934; 943; 953-960; 966-967; 987-988; 994; 1011; 1035-1042; 1064-1073; 1081-1090; 1105-1110; 1122; 1129-1133; 1139-1141; 1157-1158; 1176-1186; 1191; 1200-1201; 1221-1248; 1254-1257; 1263; 1270; 1288-1291; 1302; 1310-1314; 1324-1327; 1338-1339; 1342-1343; 1362-1364; 1378; 1391-1392; 1395; 1399-1418; 1433; 1453-1454; 1457-1459; 1466-1467; 1492-1498; 1500-1501; 1503-1504; 1522; 1528; 1533-1535; 1540-1541; 1563-1566; 1572; 1583-1586; 1593-1594; 1621-1624; 1645-1646; 1655-1658; 1670; 1674; 1681; 1687; 1701-1705; 1710-1713; 1727; 1735-1737; 1743; 1754-1756; 1761-1762; 1765; 1769; 1781; 1785; 1790; 1805; 1813; 1838-1846; 1857; 1863; 1874-1875; 1883-1884; 1897-1901; 1911-1912; 1917-1920; 1929-1930; 1937-1939; 1942-1943; or SEQ ID NO: 2N, wherein N=974-1101.

The transgenic plant that comprises the recombinant polynucleotide has a polynucleotide sequence, or a sequence that is complementary to this polynucleotide sequence, selected from any of the following:

(a) a polynucleotide sequence that encodes one of the transcription factor polypeptide sequences of Paragraph 2 of this Summary; or

(b) a polynucleotide sequence that comprises one of the polynucleotide sequences of paragraph 3 of this Summary.

The transgenic plant may also comprise a polynucleotide sequence that is a variant of the sequences in (a) and (b) that encode a polypeptide and regulate transcription, including:

(c) a sequence variant of the polynucleotide sequences of (a) or (b);

(d) an allelic variant of the polynucleotide sequences of (a) or (b);

(e) a splice variant of the polynucleotide sequences of (a) or (b);

(f) an orthologous sequence of the polynucleotide sequences of (a) or (b);

(g) a paralogous sequence of the polynucleotide sequences of (a) or (b);

(h) a polynucleotide sequence encoding a polypeptide comprising a conserved domain that exhibits at least 70% sequence homology with the polypeptide of (a), and the polypeptide comprises a conserved domain of a transcription factor that regulates transcription; or

(i) a polynucleotide sequence that hybridizes under stringent conditions to a polynucleotide sequence of one or more polynucleotides of (a) or (b), and the polynucleotide sequence encodes a polypeptide that regulates transcription.

A transcription factor sequence variant is one having at least 26% amino acid sequence similarity, or at least 40% amino acid sequence identity. A preferred transcription factor sequence variant is one having at least 50% amino acid sequence identity and a more preferred transcription factor sequence variant is one having at least 65% amino acid sequence identity to the transcription factor polypeptide sequences of paragraph 3 of this Summary, and that contains at least one functional or structural characteristic of the similar transcription factor polypeptide sequences. Sequences having lesser degrees of identity but comparable biological activity are considered to be equivalents.

The transcription factor polypeptides of the present invention include at least one conserved domain, and the portions of the polynucleotide sequences encoding the conserved domain generally exhibit at least 70% sequence identity with the aforementioned preferred polynucleotide sequences. In the case of zinc finger transcription factors, the percent identity across the conserved domain may be as low as 50%.

Various types of plants may be used to generate the transgenic plants, including soybean, wheat, corn, potato, cotton, rice, oilseed rape, sunflower, alfalfa, clover, sugarcane, turf, banana, blackberry, blueberry, strawberry, raspberry, cantaloupe, carrot, cauliflower, coffee, cucumber, eggplant, grapes, honeydew, lettuce, mango, melon, onion, papaya, peas, peppers, pineapple, pumpkin, spinach, squash, sweet corn, tobacco, tomato, watermelon, mint and other labiates, rosaceous fruits, and vegetable brassicas.

The transgenic plant may be monocotyledonous, plant, and the polynucleotide sequences used to transform the transgenic plant may be derived from either a monocot or a dicot plant. Alternatively, the transgenic plant may be a dicotyledonous plant, and the polynucleotide sequences used to transform the transgenic plant may be derived from either a monocot or a dicot plant.

These transgenic plants will generally possess traits that are altered as compared to a control plant, such as a wild-type or non-transformed plant (i.e., the non-transformed plant does not comprise the recombinant polynucleotide), thus producing an phenotype that is altered when compared to the control, wild-type or non-transformed plant. These transgenic plants may also express an altered level of one or more genes associated with a plant trait as compared to the non-transformed plant. The encoded polypeptides in these transgenic plants will generally be expressed and regulate transcription of at least one gene; this gene will generally confer at least one altered trait, phenotype or expression level.

Any of the polynucleotide sequences listed in the Sequence Listing, their complements, and functional variants used to transform the transgenic plants of the present invention may further comprise regulatory elements. The regulatory elements, may comprise, for example, constitutive, inducible, or tissue-specific promoters operably linked to a polynucleotide sequence.

Presently disclosed transcription factor sequences may be used to produce transformed plants with a variety of improved traits. An example of such an altered trait is enhanced tolerance to abiotic stress, such as salt tolerance, chilling conditions, and drought conditions. Salt and drought tolerance, both forms of osmotic stress, may be mediated in part by increased root growth or increased root hairs relative to a non-transformed, control or wild-type plant. Tolerance to abiotic stresses such as salt, chilling and drought tolerance may confer a number of survival, quality and yield improvements, including improved seed germination and improved seedling vigor, plant survival, as well as improved yield, quality, and range.

Another example of an altered trait that may be conferred by transforming plants with the presently disclosed transcription factor sequences includes altered sugar sensing. Altered sugar sensing may also be used to confer improved seed germination and improved seedling vigor, as well as altered flowering, senescence, sugar metabolism and photosynthesis characteristics.

The invention also pertains to method to produce these transgenic plants.

The present invention also relates to a method of using transgenic plants transformed with the presently disclosed transcription factor sequences, their complements or their variants to grow a progeny plant by crossing the transgenic plant with either itself or another plant, selecting seed that develops as a result of the crossing; and then growing the progeny plant from the seed. The progeny plant will generally express mRNA that encodes a transcription factor: that is, a DNA-binding protein that binds to a DNA regulatory sequence and regulates gene expression, such as that of a plant trait gene. The mRNA will generally be expressed at a level greater than a non-transformed plant; and the progeny plant is characterized by a change in a plant trait compared to the non-transformed plant.

The present invention also pertains to an expression cassette. The expression cassette comprises at least two elements, including:

(1) a constitutive, inducible, or tissue-specific promoter; and

(2) a recombinant polynucleotide having a polynucleotide sequence, or a complementary polynucleotide sequence thereof, selected from the group consisting of a polynucleotide sequence encoding a (a) polypeptide sequence selected from the transcription factor sequences in the third paragraph of this Summary; or (b) a polynucleotide sequence selected from the transcription factor polynucleotides of second paragraph of this Summary, or (c) sequence variants such as allelic or splice variants of the polynucleotide sequences of (a) or (b), where the sequence variant encodes a polypeptide that regulates transcription. The polynucleotide sequence may also comprise an orthologous or paralogous sequence of the polynucleotide sequences of (a) or (b), with these sequences encoding a polypeptide that regulates transcription, a polynucleotide sequence that encoding a polypeptide having a conserved domain that exhibits 72% or greater sequence homology with the polypeptide of (a), where the polypeptide comprising the conserved domain regulates transcription, or a polynucleotide sequence that hybridizes under stringent conditions to a polynucleotide sequence of one or more polynucleotides of (a) or (b), where the latter polynucleotide sequence regulates transcription. In all of these cases, the recombinant polynucleotide is operably linked to the promoter of the expression cassette.

The invention also includes a host cell that comprises the expression cassette. The host cell may be a plant cell, such as, for example, a cell of a crop plant.

The invention also concerns a method for identifying a factor that is modulated by or interacts with a polypeptide of the third paragraph of this Summary. This method is conducted by: expressing the polypeptide in a plant; and then identifying at least one factor that is modulated by or interacts with the polypeptide.

The invention also pertains to a method for identifying at least one downstream polynucleotide sequence that is subject to a regulatory effect of any of the polypeptides of the third paragraph of this Summary. This method includes expressing any of the polypeptides of the third paragraph of this Summary in a plant cell; and then identifying resultant RNA or protein. The latter identification may be carried out with, for example, such methods that include Northern analysis, RT-PCR, microarray gene expression assays, reporter gene expression systems subtractive hybridization, differential display, representational differential analysis, or two-dimensional gel electrophoresis of one or more protein products.

The invention also provides a transgenic plant comprising a polynucleotide encoding a polypeptide with a conserved domain, wherein the conserved domain comprises consecutive amino acid residues Ser-Ser-Lys/Arg-Tyr/Phe-Gly-Val-Val-Pro-Gln-Pro-Asn-Gly-Arg-Typ-Gly-Ala-Gln-Ile-Tyr-Glu-Lys/Arg-His-Gln-Arg-Val-Trp-Leu-Gly-Thr-Phe-Xaa-Glu/Asp-Glu-Glu/Asp-Glu/Asp-Ala-Ala/Val-Arg-Ala/Ser-Tyr-Asp-Val/Ile-Ala/Val-Val/Ala-Xaa-Arg-Phe/Tyr-Arg-Arg/Gly-Arg-Asp-Ala-Val-Thr/Val-Asn-Phe-Lys/Arg of SEQ ID NO:170, wherein Xaa is any amino acid residue. The invention still further provides a transgenic plant comprising a polynucleotide wherein the polynucleotide sequence is selected from the group consisting of SEQ ID NO: 169, 369, 1159 through 1175, 1949, and 2071. In another embodiment, the invention also provides a transgenic plant comprising a polynucleotide encoding a polypeptide, wherein the polypeptide is selected from the group consisting of SEQ ID NO: 170, 370, 1176 through 1186, 1950, and 2072.

The invention also provides an expression cassette comprising a polynucleotide encoding a polypeptide with a conserved domain, wherein the conserved domain comprises consecutive amino acid residues Ser-Ser-Lys/Arg-Tyr/Phe-Gly-Val-Val-Pro-Gln-Pro-Asn-Gly-Arg-Typ-Gly-Ala-Gln-Ile-Tyr-Glu-Lys/Arg-His-Gln-Arg-Val-Trp-Leu-Gly-Thr-Phe-Xaa-Glu/Asp-Glu-Glu/Asp-Glu/Asp-Ala-Ala/Val-Arg-Ala/Ser-Tyr-Asp-Val/Ile-Ala/Val-Val/Ala-Xaa-Arg-Phe/Tyr-Arg-Arg/Gly-Arg-Asp-Ala-Val-Thr/Val-Asn-Phe-Lys/Arg of SEQ ID NO:170, wherein Xaa is any amino acid residue. The invention still further provides an expression cassette comprising a polynucleotide sequence is selected from the group consisting of SEQ ID NO: 169, 369, 1159 through 1175, 1949, and 2071. In another embodiment, the invention also provides an expression cassette comprising a polynucleotide encoding a polypeptide, wherein the polypeptide is selected from the group consisting of SEQ ID NO: 170, 370, 1176 through 1186, 1950, and 2072.

The invention also provides a method for producing a modified plant having a polynucleotide encoding a polypeptide with a conserved domain, wherein the conserved domain comprises consecutive amino acid residues Ser-Ser-Lys/Arg-Tyr/Phe-Gly-Val-Val-Pro-Gln-Pro-Asn-Gly-Arg-Typ-Gly-Ala-Gln-Ile-Tyr-Glu-Lys/Arg-His-Gln-Arg-Val-Trp-Leu-Gly-Thr-Phe-Xaa-Glu/Asp-Glu-Glu/Asp-Glu/Asp-Ala-Ala/Val-Arg-Ala/Ser-Tyr-Asp-Val/Ile-Ala/Val-Val/Ala-Xaa-Arg-Phe/Tyr-Arg-Arg/Gly-Arg-Asp-Ala-Val-Thr/Val-Asn-Phe-Lys/Arg of SEQ ID NO:170, wherein Xaa is any amino acid residue. The invention still further provides a method for producing a modified plant having a polynucleotide, wherein the polynucleotide sequence is selected from the group consisting of SEQ ID NO: 169, 369, 1159 through 1175, 1949, and 2071. In another embodiment, the invention also provides a method for producing a modified plant having a polynucleotide encoding a polypeptide, wherein the polypeptide is selected from the group consisting of SEQ ID NO: 170, 370, 1176 through 1186, 1950, and 2072.

BRIEF DESCRIPTION OF THE SEQUENCE LISTING AND DRAWINGS

The Sequence Listing provides exemplary polynucleotide and polypeptide sequences of the invention. The traits associated with the use of the sequences are included in the Examples.

A computer-readable format (CRF) of a Sequence Listing is provided in ASCII text format. The Sequence Listing is named “MBI0047-2DIV_ST25.txt”, file creation date of Jun. 5, 2003, and is 6,258 kilobytes in size. The Sequence Listing is hereby incorporated by reference in their its entirety.

FIG. 1 shows a conservative estimate of phylogenetic relationships among the orders of flowering plants (modified from Angiosperm Phylogeny Group (1998) Ann. Missouri Bot. Gard. 84: 1-49). Those plants with a single cotyledon (monocots) are a monophyletic clade nested within at least two major lineages of dicots; the eudicots are further divided into rosids and asterids. Arabidopsis is a rosid eudicot classified within the order Brassicales; rice is a member of the monocot order Poales. FIG. 1 was adapted from Daly et al. (2001) Plant Physiol. 127: 1328-1333.

FIG. 2 shows a phylogenic dendogram depicting phylogenetic relationships of higher plant taxa, including clades containing tomato and Arabidopsis; adapted from Ku et al. (2000) Proc. Natl. Acad. Sci. 97: 9121-9126; and Chase et al. (1993) Ann. Missouri Bot. Gard. 80: 528-580.

FIGS. 3A, and 3B show an alignment of G682 (SEQ ID NO: 148) and polynucleotide sequences that are paralogous and orthologous to G682. The alignment was produced using MACVECTOR software (Accelrys, Inc., San Diego, Calif.).

FIGS. 4A, 4B, 4C and 4D show an alignment of G867 (SEQ ID NO: 170) and polynucleotide sequences that are paralogous and orthologous to G867. The alignment was produced using MACVECTOR software (Accelrys, Inc.).

FIGS. 5A, 5B, 5C, 5D, 5E and 5F show an alignment of G912 (SEQ ID NO: 186) and polynucleotide sequences that are paralogous and orthologous to G912. The alignment was produced using MACVECTOR software (Accelrys, Inc.).

DETAILED DESCRIPTION OF EXEMPLARY EMBODIMENTS

In an important aspect, the present invention relates to polynucleotides and polypeptides, for example, for modifying phenotypes of plants. Throughout this disclosure, various information sources are referred to and/or are specifically incorporated. The information sources include scientific journal articles, patent documents, textbooks, and World Wide Web browser-inactive page addresses, for example. While the reference to these information sources clearly indicates that they can be used by one of skill in the art, each and every one of the information sources cited herein are specifically incorporated in their entirety, whether or not a specific mention of “incorporation by reference” is noted. The contents and teachings of each and every one of the information sources can be relied on and used to make and use embodiments of the invention.

It must be noted that as used herein and in the appended claims, the singular forms “a,” “an,” and “the” include plural reference unless the context clearly dictates otherwise. Thus, for example, a reference to “a plant” includes a plurality of such plants, and a reference to “a stress” is a reference to one or more stresses and equivalents thereof known to those skilled in the art, and so forth.

The polynucleotide sequences of the invention encode polypeptides that are members of well-known transcription factor families, including plant transcription factor families, as disclosed in Tables 4-5. Generally, the transcription factors encoded by the present sequences are involved in cellular metabolism, cell differentiation and proliferation and the regulation of growth. Accordingly, one skilled in the art would recognize that by expressing the present sequences in a plant, one may change the expression of autologous genes or induce the expression of introduced genes. By affecting the expression of similar autologous sequences in a plant that have the biological activity of the present sequences, or by introducing the present sequences into a plant, one may alter a plant's phenotype to one with improved traits. The sequences of the invention may also be used to transform a plant and introduce desirable traits not found in the wild-type cultivar or strain. Plants may then be selected for those that produce the most desirable degree of over- or under-expression of target genes of interest and coincident trait improvement.

The sequences of the present invention may be from any species, particularly plant species, in a naturally occurring form or from any source whether natural, synthetic, semi-synthetic or recombinant. The sequences of the invention may also include fragments of the present amino acid sequences. In this context, a “fragment” refers to a fragment of a polypeptide sequence which is at least 5 to about 15 amino acids in length, most preferably at least 14 amino acids, and which retain some biological activity of a transcription factor. Where “amino acid sequence” is recited to refer to an amino acid sequence of a naturally occurring protein molecule, “amino acid sequence” and like terms are not meant to limit the amino acid sequence to the complete native amino acid sequence associated with the recited protein molecule.

As one of ordinary skill in the art recognizes, transcription factors can be identified by the presence of a region or domain of structural similarity or identity to a specific consensus sequence or the presence of a specific consensus DNA-binding site or DNA-binding site motif (see, for example, Riechmann et al. (2000) Science 290: 2105-2110). The plant transcription factors may belong to one of the following transcription factor families: the AP2 (APETALA2) domain transcription factor family (Riechmann and Meyerowitz (1998) Biol. Chem. 379: 633-646); the MYB transcription factor family (ENBib; Martin and Paz-Ares (1997) Trends Genet. 13: 67-73); the MADS domain transcription factor family (Riechmann and Meyerowitz (1997) Biol. Chem. 378: 1079-1101); the WRKY protein family (Ishiguro and Nakamura (1994) Mol. Gen. Genet. 244: 563-571); the ankyrin-repeat protein family (Zhang et al. (1992) Plant Cell 4: 1575-1588); the zinc finger protein (Z) family (Klug and Schwabe (1995) FASEB J. 9: 597-604); Takatsuji (1998) Cell. Mol. Life Sci. 54:582-596); the homeobox (HB) protein family (Buerglin (1994) in Guidebook to the Homeobox Genes. Duboule (ed.) Oxford University Press); the CAAT-element binding proteins (Forsburg and Guarente (1989) Genes Dev. 3: 1166-1178); the squamosa promoter binding proteins (SPB) (Klein et al. (1996) Mol. Gen. Genet. 1996 250: 7-16); the NAM protein family (Souer et al. (1996) Cell 85: 159-170); the IAA/AUX proteins (Abel et al. (1995) J. Mol. Biol. 251: 533-549); the HLH/MYC protein family (Littlewood et al. (1994) Prot. Profile 1: 639-709); the DNA-binding protein (DBP) family (Tucker et al. (1994) EMBO J. 13: 2994-3002); the bZIP family of transcription factors (Foster et al. (1994) FASEB J. 8: 192-200); the Box P-binding protein (the BPF-1) family (da Costa e Silva et al. (1993) Plant J. 4: 125-135); the high mobility group (HMG) family (Bustin and Reeves (1996) Prog. Nucl. Acids Res. Mol. Biol. 54: 35-100); the scarecrow (SCR) family (Di Laurenzio et al. (1996) Cell 86: 423-433); the GF14 family (Wu et al. (1997) Plant Physiol. 114: 1421-1431); the polycomb (PCOMB) family (Goodrich et al. (1997) Nature 386: 44-51); the teosinte branched (TEO) family (Luo et al. (1996) Nature 383: 794-799); the ABI3 family (Giraudat et al. (1992) Plant Cell 4: 1251-1261); the triple helix (TH) family (Dehesh et al. (1990) Science 250: 1397-1399); the EIL family (Chao et al. (1997) Cell 89: 1133-44); the AT-HOOK family (Reeves and Nissen (1990) J. Biol. Chem. 265: 8573-8582); the S1FA family (Zhou et al. (1995) Nucleic Acids Res. 23: 1165-1169); the bZIPT2 family (Lu and Ferl (1995) Plant Physiol. 109: 723); the YABBY family (Bowman et al. (1999) Development 126: 2387-96); the PAZ family (Bohmert et al. (1998) EMBO J. 17: 170-80); a family of miscellaneous (MISC) transcription factors including the DPBF family (Kim et al. (1997) Plant J. 11: 1237-1251) and the SPF1 family (Ishiguro and Nakamura (1994) Mol. Gen. Genet. 244: 563-571); the GARP family (Hall et al. (1998) Plant Cell 10: 925-936), the TUBBY family (Boggin et al (1999) Science 286: 2119-2125), the heat shock family (Wu (1995) Annu. Rev. Cell Dev. Biol. 11: 441-469), the ENBP family (Christiansen et al. (1996) Plant Mol. Biol. 32: 809-821), the RING-zinc family (Jensen et al. (1998) FEBS Letters 436: 283-287), the PDBP family (Janik et al. (1989) Virology 168: 320-329), the PCF family (Cubas et al. Plant J. (1999) 18: 215-22), the SRS(SHI-related) family (Fridborg et al. (1999) Plant Cell 11: 1019-1032), the CPP (cysteine-rich polycomb-like) family (Cvitanich et al. (2000) Proc. Natl. Acad. Sci. 97: 8163-8168), the ARF (auxin response factor) family (Ulmasov et al. (1999) Proc. Natl. Acad. Sci. 96: 5844-5849), the SWI/SNF family (Collingwood et al. (1999) J. Mol. Endocrinol. 23: 255-275), the ACBF family (Seguin et al. (1997) Plant Mol. Biol. 35: 281-291), PCGL (CG-1 like) family (da Costa e Silva et al. (1994) Plant Mol. Biol. 25: 921-924) the ARID family (Vazquez et al. (1999) Development 126: 733-742), the Jumonji family (Balciunas et al. (2000), Trends Biochem. Sci. 25: 274-276), the bZIP-NIN family (Schauser et al. (1999) Nature 402: 191-195), the E2F family (Kaelin et al. (1992) Cell 70: 351-364) and the GRF-like family (Knaap et al. (2000) Plant Physiol. 122: 695-704). As indicated by any part of the list above and as known in the art, transcription factors have been sometimes categorized by class, family, and sub-family according to their structural content and consensus DNA-binding site motif, for example. Many of the classes and many of the families and sub-families are listed here. However, the inclusion of one sub-family and not another, or the inclusion of one family and not another, does not mean that the invention does not encompass polynucleotides or polypeptides of a certain family or sub-family. The list provided here is merely an example of the types of transcription factors and the knowledge available concerning the consensus sequences and consensus DNA-binding site motifs that help define them as known to those of skill in the art (each of the references noted above are specifically incorporated herein by reference). A transcription factor may include, but is not limited to, any polypeptide that can activate or repress transcription of a single gene or a number of genes. This polypeptide group includes, but is not limited to, DNA-binding proteins, DNA-binding protein binding proteins, protein kinases, protein phosphatases, protein methyltransferases, GTP-binding proteins, and receptors, and the like.

In addition to methods for modifying a plant phenotype by employing one or more polynucleotides and polypeptides of the invention described herein, the polynucleotides and polypeptides of the invention have a variety of additional uses. These uses include their use in the recombinant production (i.e., expression) of proteins; as regulators of plant gene expression, as diagnostic probes for the presence of complementary or partially complementary nucleic acids (including for detection of natural coding nucleic acids); as substrates for further reactions, e.g., mutation reactions, PCR reactions, or the like; as substrates for cloning e.g., including digestion or ligation reactions; and for identifying exogenous or endogenous modulators of the transcription factors. A “polynucleotide” is a nucleic acid molecule comprising a plurality of polymerized nucleotides, e.g., at least about 15 consecutive polymerized nucleotides, optionally at least about 30 consecutive nucleotides, at least about 50 consecutive nucleotides. A polynucleotide may be a nucleic acid, oligonucleotide, nucleotide, or any fragment thereof. In many instances, a polynucleotide comprises a nucleotide sequence encoding a polypeptide (or protein) or a domain or fragment thereof. Additionally, the polynucleotide may comprise a promoter, an intron, an enhancer region, a polyadenylation site, a translation initiation site, 5′ or 3′ untranslated regions, a reporter gene, a selectable marker, or the like. The polynucleotide can be single stranded or double stranded DNA or RNA. The polynucleotide optionally comprises modified bases or a modified backbone. The polynucleotide can be, e.g., genomic DNA or RNA, a transcript (such as an mRNA), a cDNA, a PCR product, a cloned DNA, a synthetic DNA or RNA, or the like. The polynucleotide can be combined with carbohydrate, lipids, protein, or other materials to perform a particular activity such as transformation or form a useful composition such as a peptide nucleic acid (PNA). The polynucleotide can comprise a sequence in either sense or antisense orientations. “Oligonucleotide” is substantially equivalent to the terms amplimer, primer, oligomer, element, target, and probe and is preferably single stranded.

Definitions

A “recombinant polynucleotide” is a polynucleotide that is not in its native state, e.g., the polynucleotide comprises a nucleotide sequence not found in nature, or the polynucleotide is in a context other than that in which it is naturally found, e.g., separated from nucleotide sequences with which it typically is in proximity in nature, or adjacent (or contiguous with) nucleotide sequences with which it typically is not in proximity. For example, the sequence at issue can be cloned into a vector, or otherwise recombined with one or more additional nucleic acid.

An “isolated polynucleotide” is a polynucleotide whether naturally occurring or recombinant, that is present outside the cell in which it is typically found in nature, whether purified or not. Optionally, an isolated polynucleotide is subject to one or more enrichment or purification procedures, e.g., cell lysis, extraction, centrifugation, precipitation, or the like.

A “polypeptide” is an amino acid sequence comprising a plurality of consecutive polymerized amino acid residues e.g., at least about 15 consecutive polymerized amino acid residues, optionally at least about 30 consecutive polymerized amino acid residues, at least about 50 consecutive polymerized amino acid residues. In many instances, a polypeptide comprises a polymerized amino acid residue sequence that is a transcription factor or a domain or portion or fragment thereof. A transcription factor can regulate gene expression and may increase or decrease gene expression in a plant. Additionally, the polypeptide may comprise 1) a localization domain, 2) an activation domain, 3) a repression domain, 4) an oligomerization domain, or 5) a DNA-binding domain, or the like. The polypeptide optionally comprises modified amino acid residues, naturally occurring amino acid residues not encoded by a codon, non-naturally occurring amino acid residues.

A “recombinant polypeptide” is a polypeptide produced by translation of a recombinant polynucleotide. A “synthetic polypeptide” is a polypeptide created by consecutive polymerization of isolated amino acid residues using methods well known in the art. An “isolated polypeptide,” whether a naturally occurring or a recombinant polypeptide, is more enriched in (or out of) a cell than the polypeptide in its natural state in a wild-type cell, e.g., more than about 5% enriched, more than about 10% enriched, or more than about 20%, or more than about 50%, or more, enriched, i.e., alternatively denoted: 105%, 110%, 120%, 150% or more, enriched relative to wild type standardized at 100%. Such an enrichment is not the result of a natural response of a wild-type plant. Alternatively, or additionally, the isolated polypeptide is separated from other cellular components with which it is typically associated, e.g., by any of the various protein purification methods herein.

“Identity” or “similarity” refers to sequence similarity between two polynucleotide sequences or between two polypeptide sequences, with identity being a more strict comparison. The phrases “percent identity” and “% identity” refer to the percentage of sequence similarity found in a comparison of two or more polynucleotide sequences or two or more polypeptide sequences. “Sequence similarity” refers to the percent similarity in base pair sequence (as determined by any suitable method) between two or more polynucleotide sequences. Two or more sequences can be anywhere from 0-100% similar, or any integer value therebetween. Identity or similarity can be determined by comparing a position in each sequence that may be aligned for purposes of comparison. When a position in the compared sequence is occupied by the same nucleotide base or amino acid, then the molecules are identical at that position. A degree of similarity or identity between polynucleotide sequences is a function of the number of identical or matching nucleotides at positions shared by the polynucleotide sequences. A degree of identity of polypeptide sequences is a function of the number of identical amino acids at positions shared by the polypeptide sequences. A degree of homology or similarity of polypeptide sequences is a function of the number of amino acids at positions shared by the polypeptide sequences.

“Alignment” refers to a number of DNA or amino acid sequences aligned by lengthwise comparison so that components in common (i.e., nucleotide bases or amino acid residues) may be visually and readily identified. The fraction or percentage of components in common is related to the homology or identity between the sequences. Alignments such as those of FIG. 3, 4, or 5 may be used to identify conserved domains and relatedness within these domains. An alignment may suitably be determined by means of computer programs known in the art, such as MACVECTOR software (1999) (Accelrys, Inc., San Diego, Calif.).

The terms “highly stringent” or “highly stringent condition” refer to conditions that permit hybridization of DNA strands whose sequences are highly complementary, wherein these same conditions exclude hybridization of significantly mismatched DNAs. Polynucleotide sequences capable of hybridizing under stringent conditions with the polynucleotides of the present invention may be, for example, variants of the disclosed polynucleotide sequences, including allelic or splice variants, or sequences that encode orthologs or paralogs of presently disclosed polypeptides. Nucleic acid hybridization methods are disclosed in detail by Kashima et al. (1985) Nature 313:402-404, and Sambrook et al. (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (“Sambrook”); and by Haymes et al., “Nucleic Acid Hybridization: A Practical Approach”, IRL Press, Washington, D.C. (1985), which references are incorporated herein by reference.

In general, stringency is determined by the temperature, ionic strength, and concentration of denaturing agents (e.g., formamide) used in a hybridization and washing procedure (for a more detailed description of establishing and determining stringency, see below). The degree to which two nucleic acids hybridize under various conditions of stringency is correlated with the extent of their similarity. Thus, similar nucleic acid sequences from a variety of sources, such as within a plant's genome (as in the case of paralogs) or from another plant (as in the case of orthologs) that may perform similar functions can be isolated on the basis of their ability to hybridize with known transcription factor sequences. Numerous variations are possible in the conditions and means by which nucleic acid hybridization can be performed to isolate transcription factor sequences having similarity to transcription factor sequences known in the art and are not limited to those explicitly disclosed herein. Such an approach may be used to isolate polynucleotide sequences having various degrees of similarity with disclosed transcription factor sequences, such as, for example, transcription factors having 60% identity, or more preferably greater than about 70% identity, most preferably 72% or greater identity with disclosed transcription factors.

The term “equivalog” describes members of a set of homologous proteins that are conserved with respect to function since their last common ancestor. Related proteins are grouped into equivalog families, and otherwise into protein families with other hierarchically defined homology types. This definition is provided at the Institute for Genomic Research (TIGR) website, www.tigr.org; “Terms associated with TIGRFAMs”.

The term “variant”, as used herein, may refer to polynucleotides or polypeptides that differ from the presently disclosed polynucleotides or polypeptides, respectively, in sequence from each other, and as set forth below.

With regard to polynucleotide variants, differences between presently disclosed polynucleotides and their variants are limited so that the nucleotide sequences of the former and the latter are closely similar overall and, in many regions, identical. The degeneracy of the genetic code dictates that many different variant polynucleotides can encode identical and/or substantially similar polypeptides in addition to those sequences illustrated in the Sequence Listing. Due to this degeneracy, differences between presently disclosed polynucleotides and variant nucleotide sequences may be silent in any given region or over the entire length of the polypeptide (i.e., the amino acids encoded by the polynucleotide are the same, and the variant polynucleotide sequence thus encodes the same amino acid sequence in that region or entire length of the presently disclosed polynucleotide. Variant nucleotide sequences may encode different amino acid sequences, in which case such nucleotide differences will result in amino acid substitutions, additions, deletions, insertions, truncations or fusions with respect to the similar disclosed polynucleotide sequences. These variations result in polynucleotide variants encoding polypeptides that share at least one functional characteristic (i.e., a presently disclosed transcription factor and a variant will confer at least one of the same functions to a plant).

Within the scope of the invention is a variant of a nucleic acid listed in the Sequence Listing (except CBF polynucleotide sequences SEQ ID NOs: 1955, 1957, 1959, or 2203), that is, one having a sequence that differs from the one of the polynucleotide sequences in the Sequence Listing, or a complementary sequence, that encodes a functionally equivalent polypeptide (i.e., a polypeptide having some degree of equivalent or similar biological activity) but differs in sequence from the sequence in the Sequence Listing, due to degeneracy in the genetic code.

“Allelic variant” or “polynucleotide allelic variant” refers to any of two or more alternative forms of a gene occupying the same chromosomal locus. Allelic variation arises naturally through mutation, and may result in phenotypic polymorphism within populations. Gene mutations may be “silent” or may encode polypeptides having altered amino acid sequences. “Allelic variant” and “polypeptide allelic variant” may also be used with respect to polypeptides, and in this case the terms refer to a polypeptide encoded by an allelic variant of a gene.

“Splice variant” or “polynucleotide splice variant” as used herein refers to alternative forms of RNA transcribed from a gene. Splice variation naturally occurs as a result of alternative sites being spliced within a single transcribed RNA molecule or between separately transcribed RNA molecules, and may result in several different forms of mRNA transcribed from the same gene. Thus, splice variants may encode polypeptides having different amino acid sequences, which, in the present context, will have at least one similar function in the organism (splice variation may also give rise to distinct polypeptides having different functions). “Splice variant” or “polypeptide splice variant” may also refer to a polypeptide encoded by a splice variant of a transcribed mRNA.

As used herein, “polynucleotide variants” may also refer to polynucleotide sequences that encode paralogs and orthologs of the presently disclosed polypeptide sequences. “Polypeptide variants” may refer to polypeptide sequences that are paralogs and orthologs of the presently disclosed polypeptide sequences.

Differences between presently disclosed polypeptides and polypeptide variants are limited so that the sequences of the former and the latter are closely similar overall and, in many regions, identical. Presently disclosed polypeptide sequences and similar polypeptide variants may differ in amino acid sequence by one or more substitutions, additions, deletions, fusions and truncations, which may be present in any combination. These differences may produce silent changes and result in a functionally equivalent transcription factor. Thus, it will be readily appreciated by those of skill in the art, that any of a variety of polynucleotide sequences is capable of encoding the transcription factors and transcription factor homolog polypeptides of the invention. A polypeptide sequence variant may have “conservative” changes, wherein a substituted amino acid has similar structural or chemical properties. Deliberate amino acid substitutions may thus be made on the basis of similarity in polarity, charge, solubility, hydrophobicity, hydrophilicity, and/or the amphipathic nature of the residues, as long as the functional or biological activity of the transcription factor is retained. For example, negatively charged amino acids may include aspartic acid and glutamic acid, positively charged amino acids may include lysine and arginine, and amino acids with uncharged polar head groups having similar hydrophilicity values may include leucine, isoleucine, and valine; glycine and alanine; asparagine and glutamine; serine and threonine; and phenylalanine and tyrosine. For more detail on conservative substitutions, see Table 2. More rarely, a variant may have “non-conservative” changes, e.g., replacement of a glycine with a tryptophan. Similar minor variations may also include amino acid deletions or insertions, or both. Related polypeptides may comprise, for example, additions and/or deletions of one or more N-linked or O-linked glycosylation sites, or an addition and/or a deletion of one or more cysteine residues. Guidance in determining which and how many amino acid residues may be substituted, inserted or deleted without abolishing functional or biological activity may be found using computer programs well known in the art, for example, DNASTAR software (see U.S. Pat. No. 5,840,544).

The term “plant” includes whole plants, shoot vegetative organs/structures (e.g., leaves, stems and tubers), roots, flowers and floral organs/structures (e.g., bracts, sepals, petals, stamens, carpels, anthers and ovules), seed (including embryo, endosperm, and seed coat) and fruit (the mature ovary), plant tissue (e.g., vascular tissue, ground tissue, and the like) and cells (e.g., guard cells, egg cells, and the like), and progeny of same. The class of plants that can be used in the method of the invention is generally as broad as the class of higher and lower plants amenable to transformation techniques, including angiosperms (monocotyledonous and dicotyledonous plants), gymnosperms, ferns, horsetails, psilophytes, lycophytes, bryophytes, and multicellular algae. (See for example, FIG. 1, adapted from Daly et al. (2001) Plant Physiol. 127: 1328-1333; FIG. 2, adapted from Ku et al. (2000) Proc. Natl. Acad. Sci. 97: 9121-9126; and see also Tudge, in The Variety of Life, Oxford University Press, New York, N.Y. (2000) pp. 547-606).

A “transgenic plant” refers to a plant that contains genetic material not found in a wild-type plant of the same species, variety or cultivar. The genetic material may include a transgene, an insertional mutagenesis event (such as by transposon or T-DNA insertional mutagenesis), an activation tagging sequence, a mutated sequence, a homologous recombination event or a sequence modified by chimeraplasty. Typically, the foreign genetic material has been introduced into the plant by human manipulation, but any method can be used as one of skill in the art recognizes.

A transgenic plant may contain an expression vector or cassette. The expression cassette typically comprises a polypeptide-encoding sequence operably linked (i.e., under regulatory control of) to appropriate inducible or constitutive regulatory sequences that allow for the expression of polypeptide. The expression cassette can be introduced into a plant by transformation or by breeding after transformation of a parent plant. A plant refers to a whole plant, including seedlings and mature plants, as well as to a plant part, such as seed, fruit, leaf, or root, plant tissue, plant cells or any other plant material, e.g., a plant explant, as well as to progeny thereof, and to in vitro systems that mimic biochemical or cellular components or processes in a cell.

“Fragment”, with respect to a polynucleotide, refers to a clone or any part of a polynucleotide molecule that retains a usable, functional characteristic. Useful fragments include oligonucleotides and polynucleotides that may be used in hybridization or amplification technologies or in the regulation of replication, transcription or translation. A polynucleotide fragment” refers to any subsequence of a polynucleotide, typically, of at least about 9 consecutive nucleotides, preferably at least about 30 nucleotides, more preferably at least about 50 nucleotides, of any of the sequences provided herein. Exemplary polynucleotide fragments are the first sixty consecutive nucleotides of the transcription factor polynucleotides listed in the Sequence Listing. Exemplary fragments also include fragments that comprise a region that encodes a conserved domain of a transcription factor.

Fragments may also include subsequences of polypeptides and protein molecules, or a subsequence of the polypeptide. Fragments may have uses in that they may have antigenic potential. In some cases, the fragment or domain is a subsequence of the polypeptide that performs at least one biological function of the intact polypeptide in substantially the same manner, or to a similar extent, as does the intact polypeptide. For example, a polypeptide fragment can comprise a recognizable structural motif or functional domain such as a DNA-binding site or domain that binds to a DNA promoter region, an activation domain, or a domain for protein-protein interactions, and may initiate transcription. Fragments can vary in size from as few as 3 amino acids to the full length of the intact polypeptide, but are preferably at least about 30 amino acids in length and more preferably at least about 60 amino acids in length. Exemplary polypeptide fragments are the first twenty consecutive amino acids of a mammalian protein encoded by are the first twenty consecutive amino acids of the transcription factor polypeptides listed in the Sequence Listing.

Exemplary fragments also include fragments that comprise a conserved domain of a transcription factor. An example of such an exemplary fragment would include amino acid residues 59-124 of G867 (SEQ ID NO: 170), as noted in Table 5.

The invention also encompasses production of DNA sequences that encode transcription factors and transcription factor derivatives, or fragments thereof, entirely by synthetic chemistry. After production, the synthetic sequence may be inserted into any of the many available expression vectors and cell systems using reagents well known in the art. Moreover, synthetic chemistry may be used to introduce mutations into a sequence encoding transcription factors or any fragment thereof.

A “conserved domain” or “conserved region” as used herein refers to a region in heterologous polynucleotide or polypeptide sequences where there is a relatively high degree of sequence identity between the distinct sequences.

With respect to polynucleotides encoding presently disclosed transcription factors, a conserved region is preferably at least 10 base pairs (bp) in length.

A “conserved domain”, with respect to presently disclosed polypeptides refers to a domain within a transcription factor family that exhibits a higher degree of sequence homology, such as at least 26% sequence similarity, at least 16% sequence identity, preferably at least 40% sequence identity, preferably at least 65% sequence identity including conservative substitutions, and more preferably at least 80% sequence identity, and even more preferably at least 85%, or at least about 86%, or at least about 87%, or at least about 88%, or at least about 90%, or at least about 95%, or at least about 98% amino acid residue sequence identity of a polypeptide of consecutive amino acid residues. A fragment or domain can be referred to as outside a conserved domain, outside a consensus sequence, or outside a consensus DNA-binding site that is known to exist or that exists for a particular transcription factor class, family, or sub-family. In this case, the fragment or domain will not include the exact amino acids of a consensus sequence or consensus DNA-binding site of a transcription factor class, family or sub-family, or the exact amino acids of a particular transcription factor consensus sequence or consensus DNA-binding site. Furthermore, a particular fragment, region, or domain of a polypeptide, or a polynucleotide encoding a polypeptide, can be “outside a conserved domain” if all the amino acids of the fragment, region, or domain fall outside of a defined conserved domain(s) for a polypeptide or protein. Sequences having lesser degrees of identity but comparable biological activity are considered to be equivalents.

As one of ordinary skill in the art recognizes, conserved domains of transcription factors may be identified as regions or domains of identity to a specific consensus sequence (see, for example, Riechmann et al. (2000) supra). Thus, by using alignment methods well known in the art, the conserved domains of the plant transcription factors for each of the following may be determined: the AP2 (APETALA2) domain transcription factor family (Riechmann and Meyerowitz (1998) supra; the MYB transcription factor family (ENBib; Martin and Paz-Ares (1997) supra); the MADS domain transcription factor family (Riechmann and Meyerowitz (1997) supra); the WRKY protein family (Ishiguro and Nakamura (1994) supra); the ankyrin-repeat protein family (Zhang et al. (1992) supra); the zinc finger protein (Z) family (Klug and Schwabe (1995) supra; Takatsuji (1998) supra); the homeobox (HB) protein family (Buerglin (1994) supra); the CAAT-element binding proteins (Forsburg and Guarente (1989) supra); the squamosa promoter binding proteins (SPB) (Klein et al. (1996) supra); the NAM protein family (Souer et al. (1996) supra); the IAA/AUX proteins (Abel et al. (1995) supra); the HLH/MYC protein family (Littlewood et al. (1994) supra); the DNA-binding protein (DBP) family (Tucker et al. (1994) supra); the bZIP family of transcription factors (Foster et al. (1994) supra); the Box P-binding protein (the BPF-1) family (da Costa e Silva et al. (1993) supra); the high mobility group (HMG) family (Bustin and Reeves (1996) supra); the scarecrow (SCR) family (Di Laurenzio et al. (1996) supra); the GF14 family (Wu et al. (1997) supra); the polycomb (PCOMB) family (Goodrich et al. (1997) supra); the teosinte branched (TEO) family (Luo et al. (1996) supra); the ABI3 family (Giraudat et al. (1992) supra); the triple helix (TH) family (Dehesh et al. (1990) supra); the EIL family (Chao et al. (1997) Cell supra); the AT-HOOK family (Reeves and Nissen (1990 supra); the S1FA family (Zhou et al. (1995) supra); the bZIPT2 family (Lu and Ferl (1995) supra); the YABBY family (Bowman et al. (1999) supra); the PAZ family (Bohmert et al. (1998) supra); a family of miscellaneous (MISC) transcription factors including the DPBF family (Kim et al. (1997) supra) and the SPF1 family (Ishiguro and Nakamura (1994) supra); the GARP family (Hall et al. (1998) supra), the TUBBY family (Boggin et al. (1999) supra), the heat shock family (Wu (1995 supra), the ENBP family (Christiansen et al. (1996) supra), the RING-zinc family (Jensen et al. (1998) supra), the PDBP family (Janik et al. (1989) supra), the PCF family (Cubas et al. (1999) supra), the SRS(SHI-related) family (Fridborg et al. (1999) supra), the CPP (cysteine-rich polycomb-like) family (Cvitanich et al. (2000) supra), the ARF (auxin response factor) family (Ulmasov et al. (1999) supra), the SWI/SNF family (Collingwood et al. (1999) supra), the ACBF family (Seguin et al. (1997) supra), PCGL (CG-1 like) family (da Costa e Silva et al. (1994) supra) the ARID family (Vazquez et al. (1999) supra), the Jumonji family, (Balciunas et al. (2000) supra), the bZIP-NIN family (Schauser et al. (1999) supra), the E2F family Kaelin et al. (1992) supra) and the GRF-like family (Knaap et al (2000) supra).

The conserved domains for each of polypeptides of SEQ ID NO: 2N, wherein N=1-229, are listed in Table 5 as described in Example VII. Also, many of the polypeptides of Table 5 have conserved domains specifically indicated by start and stop sites. A comparison of the regions of the polypeptides in SEQ ID NO: 2N, wherein N=1-229, or of those in Table 5, allows one of skill in the art to identify conserved domain(s) for any of the polypeptides listed or referred to in this disclosure, including those in Tables 4-8.

As used herein, a “gene” is a functional unit of inheritance, and in physical terms is a particular segment or sequence of nucleotides along a molecule of DNA (or RNA, in the case of RNA viruses) involved in producing a functional RNA molecule, such as one used for a structural or regulatory role, or a polypeptide chain, such as one used for a structural or regulatory role (an example of the latter would be transcription regulation, as by a transcription factor polypeptide). Polypeptides may then be subjected to subsequent processing such as splicing and/or folding to obtain a functional polypeptide. A gene may be isolated, partially isolated, or be found with an organism's genome. By way of example, a transcription factor gene encodes a transcription factor polypeptide, which may be functional with or without additional processing to function as an initiator of transcription.

Operationally, genes may be defined by the cis-trans test, a genetic test that determines whether two mutations occur in the same gene and which may be used to determine the limits of the genetically active unit (Rieger et al. (1976) Glossary of Genetics and Cytogenetics: Classical and Molecular, 4th ed., Springer Verlag. Berlin). A gene generally includes regions preceding (“leaders”; upstream) and following (“trailers”; downstream) of the coding region. A gene may also include intervening, non-coded sequences, referred to as “introns”, which are located between individual coding segments, referred to as “exons”. Most genes have an identifiable associated promoter region, a regulatory sequence 5′ or upstream of the transcription initiation codon. The function of a gene may also be regulated by enhancers, operators, and other regulatory elements.

A “trait” refers to a physiological, morphological, biochemical, or physical characteristic of a plant or particular plant material or cell. In some instances, this characteristic is visible to the human eye, such as seed or plant size, or can be measured by biochemical techniques, such as detecting the protein, starch, or oil content of seed or leaves, or by observation of a metabolic or physiological process, e.g. by measuring uptake of carbon dioxide, or by the observation of the expression level of a gene or genes, e.g., by employing Northern analysis, RT-PCR, microarray gene expression assays, or reporter gene expression systems, or by agricultural observations such as stress tolerance, yield, or pathogen tolerance. Any technique can be used to measure the amount of, comparative level of, or difference in any selected chemical compound or macromolecule in the transgenic plants, however.

“Trait modification” refers to a detectable difference in a characteristic in a plant ectopically expressing a polynucleotide or polypeptide of the present invention relative to a plant not doing so, such as a wild-type plant. In some cases, the trait modification can be evaluated quantitatively. For example, the trait modification can entail at least about a 2% increase or decrease in an observed trait (difference), at least a 5% difference, at least about a 10% difference, at least about a 20% difference, at least about a 30%, at least about a 50%, at least about a 70%, or at least about a 100%, or an even greater difference compared with a wild-type plant. It is known that there can be a natural variation in the modified trait. Therefore, the trait modification observed entails a change of the normal distribution of the trait in the plants compared with the distribution observed in wild-type plant.

The term “transcript profile” refers to the expression levels of a set of genes in a cell in a particular state, particularly by comparison with the expression levels of that same set of genes in a cell of the same type in a reference state. For example, the transcript profile of a particular transcription factor in a suspension cell is the expression levels of a set of genes in a cell overexpressing that transcription factor compared with the expression levels of that same set of genes in a suspension cell that has normal levels of that transcription factor. The transcript profile can be presented as a list of those genes whose expression level is significantly different between the two treatments, and the difference ratios. Differences and similarities between expression levels may also be evaluated and calculated using statistical and clustering methods.

“Wild type”, as used herein, refers to a cell, tissue or plant that has not been genetically modified to knock out or overexpress one or more of the presently disclosed transcription factors. Wild-type cells, tissue or plants may be used as controls to compare levels of expression and the extent and nature of trait modification with modified (e.g., transgenic) cells, tissue or plants in which transcription factor expression is altered or ectopically expressed by, for example, knocking out or overexpressing a gene.

“Ectopic expression” or “altered expression” in reference to a polynucleotide indicates that the pattern of expression in, e.g., a transgenic plant or plant tissue, is different from the expression pattern in a wild-type plant or a reference plant of the same species. The pattern of expression may also be compared with a reference expression pattern in a wild-type plant of the same species. For example, the polynucleotide or polypeptide is expressed in a cell or tissue type other than a cell or tissue type in which the sequence is expressed in the wild-type plant, or by expression at a time other than at the time the sequence is expressed in the wild-type plant, or by a response to different inducible agents, such as hormones or environmental signals, or at different expression levels (either higher or lower) compared with those found in a wild-type plant. Altered expression may be achieved by, for example, transformation of a plant with an expression cassette having a constitutive or inducible promoter element associated with a transcription factor gene. The resulting expression pattern can thus constitutive or inducible, and be stable or transient. Altered or ectopic expression may also refer to altered expression patterns that are produced by lowering the levels of expression to below the detection level or completely abolishing expression by, for example, knocking out a gene's expression by disrupting expression or regulation of the gene with an insertion element.

In reference to a polypeptide, the term “ectopic expression or altered expression” further may relate to altered activity levels resulting from the interactions of the polypeptides with exogenous or endogenous modulators or from interactions with factors or as a result of the chemical modification of the polypeptides.

The term “overexpression” as used herein refers to a greater expression level of a gene in a plant, plant cell or plant tissue, compared to expression in a wild-type plant, cell or tissue, at any developmental or temporal stage for the gene. Overexpression can occur when, for example, the genes encoding one or more transcription factors are under the control of a strong expression signal, such as one of the promoters described herein (e.g., the cauliflower mosaic virus 35S transcription initiation region). Overexpression may occur throughout a plant or in specific tissues of the plant, depending on the promoter used, as described below.

Overexpression may take place in plant cells normally lacking expression of polypeptides functionally equivalent or identical to the present transcription factors. Overexpression may also occur in plant cells where endogenous expression of the present transcription factors or functionally equivalent molecules normally occurs, but such normal expression is at a lower level than in the organism or tissues of the overexpressor. Overexpression thus results in a greater than normal production, or “overproduction” of the transcription factor in the plant, cell or tissue.

The term “phase change” refers to a plant's progression from embryo to adult, and, by some definitions, the transition wherein flowering plants gain reproductive competency. It is believed that phase change occurs either after a certain number of cell divisions in the shoot apex of a developing plant, or when the shoot apex achieves a particular distance from the roots. Thus, altering the timing of phase changes may affect a plant's size, which, in turn, may affect yield and biomass.

Traits that May be Modified in Overexpressing or Knock-Out Plants

Trait modifications of particular interest include those to seed (such as embryo or endosperm), fruit, root, flower, leaf, stem, shoot, seedling or the like, including: enhanced tolerance to environmental conditions including freezing, chilling, heat, drought, water saturation, radiation and ozone; improved tolerance to microbial, fungal or viral diseases; improved tolerance to pest infestations, including insects, nematodes, mollicutes, parasitic higher plants or the like; decreased herbicide sensitivity; improved tolerance of heavy metals or enhanced ability to take up heavy metals; improved growth under poor photoconditions (e.g., low light and/or short day length), or changes in expression levels of genes of interest. Other phenotype that can be modified relate to the production of plant metabolites, such as variations in the production of taxol, tocopherol, tocotrienol, sterols, phytosterols, vitamins, wax monomers, anti-oxidants, amino acids, lignins, cellulose, tannins, prenyllipids (such as chlorophylls and carotenoids), glucosinolates, and terpenoids, enhanced or compositionally altered protein or oil production (especially in seeds), or modified sugar (insoluble or soluble) and/or starch composition. Physical plant characteristics that can be modified include cell development (such as the number of trichomes), fruit and seed size and number, yields of plant parts such as stems, leaves, inflorescences, and roots, the stability of the seeds during storage, characteristics of the seed pod (e.g., susceptibility to shattering), root hair length and quantity, internode distances, or the quality of seed coat. Plant growth characteristics that can be modified include growth rate, germination rate of seeds, vigor of plants and seedlings, leaf and flower senescence, male sterility, apomixis, flowering time, flower abscission, rate of nitrogen uptake, osmotic sensitivity to soluble sugar concentrations, biomass or transpiration characteristics, as well as plant architecture characteristics such as apical dominance, branching patterns, number of organs, organ identity, organ shape or size.

Transcription Factors Modify Expression of Endogenous Genes

Expression of genes that encode transcription factors that modify expression of endogenous genes, polynucleotides, and proteins are well known in the art. In addition, transgenic plants comprising isolated polynucleotides encoding transcription factors may also modify expression of endogenous genes, polynucleotides, and proteins. Examples include Peng et al. (1997) Genes and Development 11:3194-3205, and Peng et al. (1999) Nature 400: 256-261. In addition, many others have demonstrated that an Arabidopsis transcription factor expressed in an exogenous plant species elicits the same or very similar phenotypic response. See, for example, Fu et al. (2001) Plant Cell 13: 1791-1802; Nandi et al. (2000, Curr. Biol. 10: 215-218; Coupland (1995) Nature 377: 482-483; and Weigel and Nilsson (1995) Nature 377: 482-500.

In another example, Mandel et al. (1992) Cell 71-133-143 and Suzuki et al. (2001) Plant J. 28: 409-418, teach that a transcription factor expressed in another plant species elicits the same or very similar phenotypic response of the endogenous sequence, as often predicted in earlier studies of Arabidopsis transcription factors in Arabidopsis (see Mandel et al. (1992) supra; Suzuki et al. (2001) supra).

Other examples include Müller et al. (2001) Plant J. 28: 169-179; Kim et al. (2001) Plant J. 25: 247-259; Kyozuka and Shimamoto (2002) Plant Cell Physiol. 43: 130-135; Boss and Thomas (2002) Nature 416: 847-850; He et al. (2000) Transgenic Res. 9: 223-227; and Robson et al. (2001) Plant J. 28: 619-631.

In yet another example, Gilmour et al. (1998) Plant J. 16: 433-442, teach an Arabidopsis AP2 transcription factor, CBF1 (SEQ ID NO: 1956), which, when overexpressed in transgenic plants, increases plant freezing tolerance. Jaglo et al. (2001) Plant Physiol. 127: 910-917, further identified sequences in Brassica napus which encode CBF-like genes and that transcripts for these genes accumulated rapidly in response to low temperature. Transcripts encoding CBF-like proteins were also found to accumulate rapidly in response to low temperature in wheat, as well as in tomato. An alignment of the CBF proteins from Arabidopsis, B. napus, wheat, rye, and tomato revealed the presence of conserved consecutive amino acid residues, PKK/RPAGRxKFxETRHP (SEQ ID NO: 2907) and DSAWR (SEQ ID NO: 2908), that bracket the AP2/EREBP DNA binding domains of the proteins and distinguish them from other members of the AP2/EREBP protein family. (See Jaglo et al. supra).

Gao et al. (2002) Plant Molec. Biol. 49: 459-471) have recently described four CBF transcription factors from Brassica napus: BNCBFs 5, 7, 16 and 17. They note that the first three CBFs (GenBank Accession Numbers AAM18958, AAM18959, and AAM18960, respectively) are very similar to Arabidopsis CBF1, whereas BNCBF17 (GenBank Accession Number AAM18961) is similar but contains two extra regions of 16 and 21 amino acids in its acidic activation domain. All four B. napus CBFs accumulate in leaves of the plants after cold-treatment, and BNCBFs 5, 7, 16 accumulated after salt stress treatment. The authors concluded that these BNCBFs likely function in low-temperature responses in B. napus.

In a functional study of CBF genes, Hsieh et al. ((2002) Plant Physiol. 129: 1086-1094) found that heterologous expression of Arabidopsis CBF1 in tomato plants confers increased tolerance to chilling and considerable tolerance to oxidative stress, which suggested to the authors that ectopic Arabidopsis CBF1 expression may induce several tomato stress responsive genes to protect the plants.

Polypeptides and Polynucleotides of the Invention

The present invention provides, among other things, transcription factors (TFs), and transcription factor homolog polypeptides, and isolated or recombinant polynucleotides encoding the polypeptides, or novel sequence variant polypeptides or polynucleotides encoding novel variants of transcription factors derived from the specific sequences provided here. These polypeptides and polynucleotides may be employed to modify a plant's characteristics.

Exemplary polynucleotides encoding the polypeptides of the invention were identified in the Arabidopsis thaliana GenBank database using publicly available sequence analysis programs and parameters. Sequences initially identified were then further characterized to identify sequences comprising specified sequence strings corresponding to sequence motifs present in families of known transcription factors. In addition, further exemplary polynucleotides encoding the polypeptides of the invention were identified in the plant GenBank database using publicly available sequence analysis programs and parameters. Sequences initially identified were then further characterized to identify sequences comprising specified sequence strings corresponding to sequence motifs present in families of known transcription factors. Polynucleotide sequences meeting such criteria were confirmed as transcription factors.

Additional polynucleotides of the invention were identified by screening Arabidopsis thaliana and/or other plant cDNA libraries with probes corresponding to known transcription factors under low stringency hybridization conditions. Additional sequences, including full length coding sequences were subsequently recovered by the rapid amplification of cDNA ends (RACE) procedure, using a commercially available kit according to the manufacturer's instructions. Where necessary, multiple rounds of RACE are performed to isolate 5′ and 3′ ends. The full-length cDNA was then recovered by a routine end-to-end polymerase chain reaction (PCR) using primers specific to the isolated 5′ and 3′ ends. Exemplary sequences are provided in the Sequence Listing.

The polynucleotides of the invention can be or were ectopically expressed in overexpressor or knockout plants and the changes in the characteristic(s) or trait(s) of the plants observed. Therefore, the polynucleotides and polypeptides can be employed to improve the characteristics of plants.

The polynucleotides of the invention can be or were ectopically expressed in overexpressor plant cells and the changes in the expression levels of a number of genes, polynucleotides, and/or proteins of the plant cells observed. Therefore, the polynucleotides and polypeptides can be employed to change expression levels of a genes, polynucleotides, and/or proteins of plants.

Producing Polypeptides

The polynucleotides of the invention include sequences that encode transcription factors and transcription factor homolog polypeptides and sequences complementary thereto, as well as unique fragments of coding sequence, or sequence complementary thereto. Such polynucleotides can be, e.g., DNA or RNA, e.g., mRNA, cRNA, synthetic RNA, genomic DNA, cDNA synthetic DNA, oligonucleotides, etc. The polynucleotides are either double-stranded or single-stranded, and include either, or both sense (i.e., coding) sequences and antisense (i.e., non-coding, complementary) sequences. The polynucleotides include the coding sequence of a transcription factor, or transcription factor homolog polypeptide, in isolation, in combination with additional coding sequences (e.g., a purification tag, a localization signal, as a fusion-protein, as a pre-protein, or the like), in combination with non-coding sequences (e.g., introns or inteins, regulatory elements such as promoters, enhancers, terminators, and the like), and/or in a vector or host environment in which the polynucleotide encoding a transcription factor or transcription factor homolog polypeptide is an endogenous or exogenous gene.

A variety of methods exist for producing the polynucleotides of the invention. Procedures for identifying and isolating DNA clones are well known to those of skill in the art, and are described in, e.g., Berger and Kimmel, Guide to Molecular Cloning Techniques, Methods in Enzymology, vol. 152 Academic Press, Inc., San Diego, Calif. (“Berger”); Sambrook et al. (1989) Molecular Cloning—A Laboratory Manual (2nd Ed.), Vol. 1-3, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., and Current Protocols in Molecular Biology, Ausubel et al. eds., Current Protocols, a joint venture between Greene Publishing Associates, Inc. and John Wiley & Sons, Inc., (supplemented through 2000) (“Ausubel”).

Alternatively, polynucleotides of the invention, can be produced by a variety of in vitro amplification methods adapted to the present invention by appropriate selection of specific or degenerate primers. Examples of protocols sufficient to direct persons of skill through in vitro amplification methods, including the polymerase chain reaction (PCR) the ligase chain reaction (LCR), Qbeta-replicase amplification and other RNA polymerase mediated techniques (e.g., NASBA), e.g., for the production of the homologous nucleic acids of the invention are found in Berger (supra), Sambrook (supra), and Ausubel (supra), as well as Mullis et al. (1987) PCR Protocols A Guide to Methods and Applications (Innis et al. eds) Academic Press Inc. San Diego, Calif. (1990) (Innis). Improved methods for cloning in vitro amplified nucleic acids are described in Wallace et al. U.S. Pat. No. 5,426,039. Improved methods for amplifying large nucleic acids by PCR are summarized in Cheng et al. (1994) Nature 369: 684-685 and the references cited therein, in which PCR amplicons of up to 40 kb are generated. One of skill will appreciate that essentially any RNA can be converted into a double stranded DNA suitable for restriction digestion, PCR expansion and sequencing using reverse transcriptase and a polymerase. See, e.g., Ausubel, Sambrook and Berger, all supra.

Alternatively, polynucleotides and oligonucleotides of the invention can be assembled from fragments produced by solid-phase synthesis methods. Typically, fragments of up to approximately 100 bases are individually synthesized and then enzymatically or chemically ligated to produce a desired sequence, e.g., a polynucleotide encoding all or part of a transcription factor. For example, chemical synthesis using the phosphoramidite method is described, e.g., by Beaucage et al. (1981) Tetrahedron Letters 22: 1859-1869; and Matthes et al. (1984) EMBO J. 3: 801-805. According to such methods, oligonucleotides are synthesized, purified, annealed to their complementary strand, ligated and then optionally cloned into suitable vectors. And if so desired, the polynucleotides and polypeptides of the invention can be custom ordered from any of a number of commercial suppliers.

Homologous Sequences

Sequences homologous, i.e., that share significant sequence identity or similarity, to those provided in the Sequence Listing (except CBF sequences SEQ ID NOs: 1955-1960), derived from Arabidopsis thaliana or from other plants of choice, are also an aspect of the invention. Homologous sequences can be derived from any plant including monocots and dicots and in particular agriculturally important plant species, including but not limited to, crops such as soybean, wheat, corn (maize), potato, cotton, rice, rape, oilseed rape (including canola), sunflower, alfalfa, clover, sugarcane, and turf; or fruits and vegetables, such as banana, blackberry, blueberry, strawberry, and raspberry, cantaloupe, carrot, cauliflower, coffee, cucumber, eggplant, grapes, honeydew, lettuce, mango, melon, onion, papaya, peas, peppers, pineapple, pumpkin, spinach, squash, sweet corn, tobacco, tomato, tomatillo, watermelon, rosaceous fruits (such as apple, peach, pear, cherry and plum) and vegetable brassicas (such as broccoli, cabbage, cauliflower, Brussels sprouts, and kohlrabi). Other crops, including fruits and vegetables, whose phenotype can be changed and which comprise homologous sequences include barley; rye; millet; sorghum; currant; avocado; citrus fruits such as oranges, lemons, grapefruit and tangerines, artichoke, cherries; nuts such as the walnut and peanut; endive; leek; roots such as arrowroot, beet, cassava, turnip, radish, yam, and sweet potato; and beans. The homologous sequences may also be derived from woody species, such pine, poplar and eucalyptus, or mint or other labiates. In addition, homologous sequences may be derived from plants that are evolutionarily-related to crop plants, but which may not have yet been used as crop plants. Examples include deadly nightshade (Atropa belladona), related to tomato; jimson weed (Datura strommium), related to peyote; and teosinte (Zea species), related to corn (maize).

Orthologs and Paralogs

Homologous sequences as described above can comprise orthologous or paralogous sequences. Several different methods are known by those of skill in the art for identifying and defining these functionally homologous sequences. Three general methods for defining orthologs and paralogs are described; an ortholog or paralog, including equivalogs, may be identified by one or more of the methods described below.

Orthologs and paralogs are evolutionarily related genes that have similar sequence and similar functions. Orthologs are structurally related genes in different species that are derived by a speciation event. Paralogs are structurally related genes within a single species that are derived by a duplication event.

Within a single plant species, gene duplication may cause two copies of a particular gene, giving rise to two or more genes with similar sequence and often similar function known as paralogs. A paralog is therefore a similar gene formed by duplication within the same species. Paralogs typically cluster together or in the same clade (a group of similar genes) when a gene family phylogeny is analyzed using programs such as CLUSTAL (Thompson et al. (1994) Nucleic Acids Res. 22: 4673-4680; Higgins et al. (1996) Methods Enzymol. 266: 383-402). Groups of similar genes can also be identified with pair-wise BLAST analysis (Feng and Doolittle (1987) J. Mol. Evol. 25: 351-360). For example, a clade of very similar MADS domain transcription factors from Arabidopsis all share a common function in flowering time (Ratcliffe et al. (2001) Plant Physiol. 126: 122-132), and a group of very similar AP2 domain transcription factors from Arabidopsis are involved in tolerance of plants to freezing (Gilmour et al. (1998) Plant J. 16: 433-442). Analysis of groups of similar genes with similar function that fall within one clade can yield sub-sequences that are particular to the clade. These sub-sequences, known as consensus sequences, can not only be used to define the sequences within each clade, but define the functions of these genes; genes within a clade may contain paralogous sequences, or orthologous sequences that share the same function (see also, for example, Mount (2001), in Bioinformatics: Sequence and Genome Analysis, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., page 543.)

Speciation, the production of new species from a parental species, can also give rise to two or more genes with similar sequence and similar function. These genes, termed orthologs, often have an identical function within their host plants and are often interchangeable between species without losing function. Because plants have common ancestors, many genes in any plant species will have a corresponding orthologous gene in another plant species. Once a phylogenic tree for a gene family of one species has been constructed using a program such as CLUSTAL (Thompson et al. (1994) Nucleic Acids Res. 22: 4673-4680; Higgins et al. (1996) supra) potential orthologous sequences can be placed into the phylogenetic tree and their relationship to genes from the species of interest can be determined. Orthologous sequences can also be identified by a reciprocal BLAST strategy. Once an orthologous sequence has been identified, the function of the ortholog can be deduced from the identified function of the reference sequence.

Transcription factor gene sequences are conserved across diverse eukaryotic species lines (Goodrich et al. (1993) Cell 75: 519-530; Lin et al. (1991) Nature 353: 569-571; Sadowski et al. (1988) Nature 335: 563-564). et al. Plants are no exception to this observation; diverse plant species possess transcription factors that have similar sequences and functions.

Orthologous genes from different organisms have highly conserved functions, and very often essentially identical functions (Lee et al. (2002) Genome Res. 12: 493-502; Remm et al. (2001) J. Mol. Biol. 314: 1041-1052). Paralogous genes, which have diverged through gene duplication, may retain similar functions of the encoded proteins. In such cases, paralogs can be used interchangeably with respect to certain embodiments of the instant invention (for example, transgenic expression of a coding sequence). An example of such highly related paralogs is the CBF family, with three well-defined members in Arabidopsis and at least one ortholog in Brassica napus (SEQ ID NOs: 1956, 1958, 1960, or 2204, respectively), all of which control pathways involved in both freezing and drought stress (Gilmour et al. (1998) Plant J. 16: 433-442; Jaglo et al. (1998) Plant Physiol. 127: 910-917).

The following references represent a small sampling of the many studies that demonstrate that conserved transcription factor genes from diverse species are likely to function similarly (i.e., regulate similar target sequences and control the same traits), and that transcription factors may be transformed into diverse species to confer or improve traits.

(1) The Arabidopsis NPR1 gene regulates systemic acquired resistance (SAR); over-expression of NPR1 leads to enhanced resistance in Arabidopsis. When either Arabidopsis NPR1 or the rice NPR1 ortholog was overexpressed in rice (which, as a monocot, is diverse from Arabidopsis), challenge with the rice bacterial blight pathogen Xanthomonas oryzae pv. Oryzae, the transgenic plants displayed enhanced resistance (Chern et al. (2001) Plant J. 27: 101-113). NPR1 acts through activation of expression of transcription factor genes, such as TGA2 (Fan and Dong (2002) Plant Cell 14: 1377-1389).

(2) E2F genes are involved in transcription of plant genes for proliferating cell nuclear antigen (PCNA). Plant E2Fs share a high degree of similarity in amino acid sequence between monocots and dicots, and are even similar to the conserved domains of the animal E2Fs. Such conservation indicates a functional similarity between plant and animal E2Fs. E2F transcription factors that regulate meristem development act through common cis-elements, and regulate related (PCNA) genes (Kosugi and Ohashi, (2002) Plant J. 29: 45-59).

(3) The ABI5 gene (abscisic acid (ABA) insensitive 5) encodes a basic leucine zipper factor required for ABA response in the seed and vegetative tissues. Co-transformation experiments with ABI5 cDNA constructs in rice protoplasts resulted in specific transactivation of the ABA-inducible wheat, Arabidopsis, bean, and barley promoters. These results demonstrate that sequentially similar ABI5 transcription factors are key targets of a conserved ABA signaling pathway in diverse plants. (Gampala et al. (2001) J. Biol. Chem. 277: 1689-1694).

(4) Sequences of three Arabidopsis GAMYB-like genes were obtained on the basis of sequence similarity to GAMYB genes from barley, rice, and L. temulentum. These three Arabadopsis genes were determined to encode transcription factors (AtMYB33, AtMYB65, and AtMYB101) and could substitute for a barley GAMYB and control alpha-amylase expression (Gocal et al. (2001) Plant Physiol. 127: 1682-1693).

(5) The floral control gene LEAFY from Arabidopsis can dramatically accelerate flowering in numerous dicotyledonous plants. Constitutive expression of Arabidopsis LEAFY also caused early flowering in transgenic rice (a monocot), with a heading date that was 26-34 days earlier than that of wild-type plants. These observations indicate that floral regulatory genes from Arabidopsis are useful tools for heading date improvement in cereal crops (He et al. (2000) Transgenic Res. 9: 223-227).

(6) Bioactive gibberellins (GAs) are essential endogenous regulators of plant growth. GA signaling tends to be conserved across the plant kingdom. GA signaling is mediated via GAI, a nuclear member of the GRAS family of plant transcription factors. Arabidopsis GAI has been shown to function in rice to inhibit gibberellin response pathways (Fu et al. (2001) Plant Cell 13: 1791-1802).

(7) The Arabidopsis gene SUPERMAN (SUP), encodes a putative transcription factor that maintains the boundary between stamens and carpels. By over-expressing Arabidopsis SUP in rice, the effect of the gene's presence on whorl boundaries was shown to be conserved. This demonstrated that SUP is a conserved regulator of floral whorl boundaries and affects cell proliferation (Nandi et al. (2000) Curr. Biol. 10: 215-218).

(8) Maize, petunia and Arabidopsis myb transcription factors that regulate flavonoid biosynthesis are very genetically similar and affect the same trait in their native species, therefore sequence and function of these myb transcription factors correlate with each other in these diverse species (Borevitz et al. (2000) Plant Cell 12: 2383-2394).

(9) Wheat reduced height-1 (Rht-B1/Rht-D1) and maize dwarf-8 (d8) genes are orthologs of the Arabidopsis gibberellin insensitive (GAI) gene. Both of these genes have been used to produce dwarf grain varieties that have improved grain yield. These genes encode proteins that resemble nuclear transcription factors and contain an SH2-like domain, indicating that phosphotyrosine may participate in gibberellin signaling. Transgenic rice plants containing a mutant GAI allele from Arabidopsis have been shown to produce reduced responses to gibberellin and are dwarfed, indicating that mutant GAI orthologs could be used to increase yield in a wide range of crop species (Peng et al. (1999) Nature 400: 256-261).

Transcription factors that are homologous to the listed sequences will typically share, in at least one conserved domain, at least about 70% amino acid sequence identity, and with regard to zinc finger transcription factors, at least about 50% amino acid sequence identity. More closely related transcription factors can share at least about 70%, or about 75% or about 80% or about 90% or about 95% or about 98% or more sequence identity with the listed sequences, or with the listed sequences but excluding or outside a known consensus sequence or consensus DNA-binding site, or with the listed sequences excluding one or all conserved domain. Factors that are most closely related to the listed sequences share, e.g., at least about 85%, about 90% or about 95% or more % sequence identity to the listed sequences, or to the listed sequences but excluding or outside a known consensus sequence or consensus DNA-binding site or outside one or all conserved domain. At the nucleotide level, the sequences will typically share at least about 40% nucleotide sequence identity, preferably at least about 50%, about 60%, about 70% or about 80% sequence identity, and more preferably about 85%, about 90%, about 95% or about 97% or more sequence identity to one or more of the listed sequences, or to a listed sequence but excluding or outside a known consensus sequence or consensus DNA-binding site, or outside one or all conserved domain. The degeneracy of the genetic code enables major variations in the nucleotide sequence of a polynucleotide while maintaining the amino acid sequence of the encoded protein. Conserved domains within a transcription factor family may exhibit a higher degree of sequence homology, such as at least 65% amino acid sequence identity including conservative substitutions, and preferably at least 80% sequence identity, and more preferably at least 85%, or at least about 86%, or at least about 87%, or at least about 88%, or at least about 90%, or at least about 95%, or at least about 98% sequence identity. Transcription factors that are homologous to the listed sequences should share at least 30%, or at least about 60%, or at least about 75%, or at least about 80%, or at least about 90%, or at least about 95% amino acid sequence identity over the entire length of the polypeptide or the homolog.

Percent identity can be determined electronically, e.g., by using the MEGALIGN program (DNASTAR, Inc. Madison, Wis.). The MEGALIGN program can create alignments between two or more sequences according to different methods, for example, the clustal method. (See, for example, Higgins and Sharp (1988) Gene 73: 237-244.) The clustal algorithm groups sequences into clusters by examining the distances between all pairs. The clusters are aligned pairwise and then in groups. Other alignment algorithms or programs may be used, including FASTA, BLAST, or ENTREZ, FASTA and BLAST, and which may be used to calculate percent similarity. These are available as a part of the GCG sequence analysis package (University of Wisconsin, Madison, Wis.), and can be used with or without default settings. ENTREZ is available through the National Center for Biotechnology Information. In one embodiment, the percent identity of two sequences can be determined by the GCG program with a gap weight of 1, e.g., each amino acid gap is weighted as if it were a single amino acid or nucleotide mismatch between the two sequences (see U.S. Pat. No. 6,262,333).

Other techniques for alignment are described in Doolittle, R. F. (1996) Methods in Enzymology: Computer Methods for Macromolecular Sequence Analysis, vol. 266, Academic Press, Orlando, Fla., USA. Preferably, an alignment program that permits gaps in the sequence is utilized to align the sequences. The Smith-Waterman is one type of algorithm that permits gaps in sequence alignments (see Shpaer (1997) Methods Mol. Biol. 70: 173-187). Also, the GAP program using the Needleman and Wunsch alignment method can be utilized to align sequences. An alternative search strategy uses MPSRCH software, which runs on a MASPAR computer. MPSRCH uses a Smith-Waterman algorithm to score sequences on a massively parallel computer. This approach improves ability to pick up distantly related matches, and is especially tolerant of small gaps and nucleotide sequence errors. Nucleic acid-encoded amino acid sequences can be used to search both protein and DNA databases.

The percentage similarity between two polypeptide sequences, e.g., sequence A and sequence B, is calculated by dividing the length of sequence A, minus the number of gap residues in sequence A, minus the number of gap residues in sequence B, into the sum of the residue matches between sequence A and sequence B, times one hundred. Gaps of low or of no similarity between the two amino acid sequences are not included in determining percentage similarity. Percent identity between polynucleotide sequences can also be counted or calculated by other methods known in the art, e.g., the Jotun Hein method. (See, e.g., Hein (1990) Methods Enzymol. 183: 626-645.) Identity between sequences can also be determined by other methods known in the art, e.g., by varying hybridization conditions (see US Patent Application No. 20010010913).

The percent identity between two conserved domains of a transcription factor DNA-binding domain consensus polypeptide sequence can be as low as 16%, as exemplified in the case of GATA1 family of eukaryotic Cys₂/Cys₂-type zinc finger transcription factors. The DNA-binding domain consensus polypeptide sequence of the GATA1 family is CX₂CX₁₇CX₂C, where X is any amino acid residue. (See, for example, Takatsuji, supra.) Other examples of such conserved consensus polypeptide sequences with low overall percent sequence identity are well known to those of skill in the art.

Thus, the invention provides methods for identifying a sequence similar or paralogous or orthologous or homologous to one or more polynucleotides as noted herein, or one or more target polypeptides encoded by the polynucleotides, or otherwise noted herein and may include linking or associating a given plant phenotype or gene function with a sequence. In the methods, a sequence database is provided (locally or across an internet or intranet) and a query is made against the sequence database using the relevant sequences herein and associated plant phenotypes or gene functions.

In addition, one or more polynucleotide sequences or one or more polypeptides encoded by the polynucleotide sequences may be used to search against a BLOCKS (Bairoch et al. (1997) Nucleic Acids Res. 25: 217-221), PFAM, and other databases which contain previously identified and annotated motifs, sequences and gene functions. Methods that search for primary sequence patterns with secondary structure gap penalties (Smith et al. (1992) Protein Engineering 5: 35-51) as well as algorithms such as Basic Local Alignment Search Tool (BLAST; Altschul (1993) J. Mol. Evol. 36: 290-300; Altschul et al. (1990) supra), BLOCKS (Henikoff and Henikoff (1991) Nucleic Acids Res. 19: 6565-6572), Hidden Markov Models (HMM; Eddy (1996) Curr. Opin. Str. Biol. 6: 361-365; Sonnhammer et al. (1997) Proteins 28: 405-420), and the like, can be used to manipulate and analyze polynucleotide and polypeptide sequences encoded by polynucleotides. These databases, algorithms and other methods are well known in the art and are described in Ausubel et al. (1997; Short Protocols in Molecular Biology, John Wiley & Sons, New York, N.Y., unit 7.7) and in Meyers (1995; Molecular Biology and Biotechnology, Wiley VCH, New York, N.Y., p 856-853).

Furthermore, methods using manual alignment of sequences similar or homologous to one or more polynucleotide sequences or one or more polypeptides encoded by the polynucleotide sequences may be used to identify regions of similarity and conserved domains. Such manual methods are well-known of those of skill in the art and can include, for example, comparisons of tertiary structure between a polypeptide sequence encoded by a polynucleotide which comprises a known function with a polypeptide sequence encoded by a polynucleotide sequence which has a function not yet determined. Such examples of tertiary structure may comprise predicted alpha helices, beta-sheets, amphipathic helices, leucine zipper motifs, zinc finger motifs, proline-rich regions, cysteine repeat motifs, and the like.

Orthologs and paralogs of presently disclosed transcription factors may be cloned using compositions provided by the present invention according to methods well known in the art. cDNAs can be cloned using mRNA from a plant cell or tissue that expresses one of the present transcription factors. Appropriate mRNA sources may be identified by interrogating Northern blots with probes designed from the present transcription factor sequences, after which a library is prepared from the mRNA obtained from a positive cell or tissue. Transcription factor-encoding cDNA is then isolated using, for example, PCR, using primers designed from a presently disclosed transcription factor gene sequence, or by probing with a partial or complete cDNA or with one or more sets of degenerate probes based on the disclosed sequences. The cDNA library may be used to transform plant cells. Expression of the cDNAs of interest is detected using, for example, methods disclosed herein such as microarrays, Northern blots, quantitative PCR, or any other technique for monitoring changes in expression. Genomic clones may be isolated using similar techniques to those.

Identifying Polynucleotides or Nucleic Acids by Hybridization

Polynucleotides homologous to the sequences illustrated in the Sequence Listing and tables can be identified, e.g., by hybridization to each other under stringent or under highly stringent conditions. Single stranded polynucleotides hybridize when they associate based on a variety of well characterized physical-chemical forces, such as hydrogen bonding, solvent exclusion, base stacking and the like. The stringency of a hybridization reflects the degree of sequence identity of the nucleic acids involved, such that the higher the stringency, the more similar are the two polynucleotide strands. Stringency is influenced by a variety of factors, including temperature, salt concentration and composition, organic and non-organic additives, solvents, etc. present in both the hybridization and wash solutions and incubations (and number thereof), as described in more detail in the references cited above.

Encompassed by the invention are polynucleotide sequences that are capable of hybridizing to the claimed polynucleotide sequences, including any of the transcription factor polynucleotides within the Sequence Listing (excluding CBF sequences SEQ ID NOs: 1955, 1957, 1959, or 2203), and fragments thereof under various conditions of stringency (See, for example, Wahl and Berger (1987) Methods Enzymol. 152: 399-407; and Kimmel (1987) Methods Enzymol. 152: 507-511). In addition to the nucleotide sequences listed in Tables 4 and 5, full length cDNA, orthologs, and paralogs of the present nucleotide sequences may be identified and isolated using well-known methods. The cDNA libraries orthologs, and paralogs of the present nucleotide sequences may be screened using hybridization methods to determine their utility as hybridization target or amplification probes.

With regard to hybridization, conditions that are highly stringent, and means for achieving them, are well known in the art. See, for example, Sambrook et al. (1989) “Molecular Cloning: A Laboratory Manual” (2nd ed., Cold Spring Harbor Laboratory); Berger and Kimmel, eds., (1987) “Guide to Molecular Cloning Techniques”, In Methods in Enzymology: 152: 467-469; and Anderson and Young (1985) “Quantitative Filter Hybridisation.” In: Hames and Higgins, ed., Nucleic Acid Hybridisation, A Practical Approach. Oxford, IRL Press, 73-111.

Stability of DNA duplexes is affected by such factors as base composition, length, and degree of base pair mismatch. Hybridization conditions may be adjusted to allow DNAs of different sequence relatedness to hybridize. The melting temperature (T_(m)) is defined as the temperature when 50% of the duplex molecules have dissociated into their constituent single strands. The melting temperature of a perfectly matched duplex, where the hybridization buffer contains formamide as a denaturing agent, may be estimated by the following equation: DNA-DNA: T _(m)(° C.)=81.5+16.6(log [Na+])+0.41(% G+C)−0.62(% formamide)−500/L  (1) DNA-RNA: T _(m)(° C.)=79.8+18.5(log [Na+])+0.58(% G+C)+0.12(% G+C)²−0.5(% formamide)−820/L  (2) RNA-RNA: T _(m)(° C.)=79.8+18.5(log [Na+])+0.58(% G+C)+0.12(% G+C)²−0.35(% formamide)−820/L  (3)

where L is the length of the duplex formed, [Na+] is the molar concentration of the sodium ion in the hybridization or washing solution, and % G+C is the percentage of (guanine+cytosine) bases in the hybrid. For imperfectly matched hybrids, approximately 1° C. is required to reduce the melting temperature for each 1-% mismatch.

Hybridization experiments are generally conducted in a buffer of pH between 6.8 to 7.4, although the rate of hybridization is nearly independent of pH at ionic strengths likely to be used in the hybridization buffer (Anderson et al. (1985) supra). In addition, one or more of the following may be used to reduce non-specific hybridization: sonicated salmon sperm DNA or another non-complementary DNA, bovine serum albumin, sodium pyrophosphate, sodium dodecylsulfate (SDS), polyvinyl-pyrrolidone, ficoll and Denhardt's solution. Dextran sulfate and polyethylene glycol 6000 act to exclude DNA from solution, thus raising the effective probe DNA concentration and the hybridization signal within a given unit of time. In some instances, conditions of even greater stringency may be desirable or required to reduce non-specific and/or background hybridization. These conditions may be created with the use of higher temperature, lower ionic strength and higher concentration of a denaturing agent such as formamide.

Stringency conditions can be adjusted to screen for moderately similar fragments such as homologous sequences from distantly related organisms, or to highly similar fragments such as genes that duplicate functional enzymes from closely related organisms. The stringency can be adjusted either during the hybridization step or in the post-hybridization washes. Salt concentration, formamide concentration, hybridization temperature and probe lengths are variables that can be used to alter stringency (as described by the formula above). As a general guidelines high stringency is typically performed at T_(m)−5° C. to T_(m)−20° C., moderate stringency at T_(m)−20° C. to T_(m)−35° C. and low stringency at T_(m)−35° C. to T_(m)−50° C. for duplex >150 base pairs. Hybridization may be performed at low to moderate stringency (25-50° C. below T_(m)), followed by post-hybridization washes at increasing stringencies. Maximum rates of hybridization in solution are determined empirically to occur at T_(m)−25° C. for DNA-DNA duplex and T_(m)−15° C. for RNA-DNA duplex. Optionally, the degree of dissociation may be assessed after each wash step to determine the need for subsequent, higher stringency wash steps.

High stringency conditions may be used to select for nucleic acid sequences with high degrees of identity to the disclosed sequences. An example of stringent hybridization conditions obtained in a filter-based method such as a Southern or northern blot for hybridization of complementary nucleic acids that have more than 100 complementary residues is about 5° C. to 20° C. lower than the thermal melting point (T_(m)) for the specific sequence at a defined ionic strength and pH. Conditions used for hybridization may include about 0.02 M to about 0.15 M sodium chloride, about 0.5% to about 5% casein, about 0.02% SDS or about 0.1% N-laurylsarcosine, about 0.001 M to about 0.03 M sodium citrate, at hybridization temperatures between about 50° C. and about 70° C. More preferably, high stringency conditions are about 0.02 M sodium chloride, about 0.5% casein, about 0.02% SDS, about 0.001 M sodium citrate, at a temperature of about 50° C. Nucleic acid molecules that hybridize under stringent conditions will typically hybridize to a probe based on either the entire DNA molecule or selected portions, e.g., to a unique subsequence, of the DNA.

Stringent salt concentration will ordinarily be less than about 750 mM NaCl and 75 mM trisodium citrate. Increasingly stringent conditions may be obtained with less than about 500 mM NaCl and 50 mM trisodium citrate, to even greater stringency with less than about 250 mM NaCl and 25 mM trisodium citrate. Low stringency hybridization can be obtained in the absence of organic solvent, e.g., formamide, whereas high stringency hybridization may be obtained in the presence of at least about 35% formamide, and more preferably at least about 50% formamide. Stringent temperature conditions will ordinarily include temperatures of at least about 30° C., more preferably of at least about 37° C., and most preferably of at least about 42° C. with formamide present. Varying additional parameters, such as hybridization time, the concentration of detergent, e.g., sodium dodecyl sulfate (SDS) and ionic strength, are well known to those skilled in the art. Various levels of stringency are accomplished by combining these various conditions as needed. In a preferred embodiment, hybridization will occur at 30° C. in 750 mM NaCl, 75 mM trisodium citrate, and 1% SDS. In a more preferred embodiment, hybridization will occur at 37° C. in 500 mM NaCl, 50 mM trisodium citrate, 1% SDS, 35% formamide. In a most preferred embodiment, hybridization will occur at 42° C. in 250 mM NaCl, 25 mM trisodium citrate, 1% SDS, 50% formamide. Useful variations on these conditions will be readily apparent to those skilled in the art.

The washing steps that follow hybridization may also vary in stringency; the post-hybridization wash steps primarily determine hybridization specificity, with the most critical factors being temperature and the ionic strength of the final wash solution. Wash stringency can be increased by decreasing salt concentration or by increasing temperature. Stringent salt concentration for the wash steps will preferably be less than about 30 mM NaCl and 3 mM trisodium citrate, and most preferably less than about 15 mM NaCl and 1.5 mM trisodium citrate. For example, the wash conditions may be under conditions of 0.1×SSC to 2.0×SSC and 0.1% SDS at 50-65° C., with, for example, two steps of 10-30 min. One example of stringent wash conditions includes about 2.0×SSC, 0.1% SDS at 65° C. and washing twice, each wash step being about 30 min. A higher stringency wash is about 0.2×SSC, 0.1% SDS at 65° C. and washing twice for 30 min. A still higher stringency wash is about 0.1×SSC, 0.1% SDS at 65° C. and washing twice for 30 min. The temperature for the wash solutions will ordinarily be at least about 25° C., and for greater stringency at least about 42° C. Hybridization stringency may be increased further by using the same conditions as in the hybridization steps, with the wash temperature raised about 3° C. to about 5° C., and stringency may be increased even further by using the same conditions except the wash temperature is raised about 6° C. to about 9° C. For identification of less closely related homolog, wash steps may be performed at a lower temperature, e.g., 50° C.

An example of a low stringency wash step employs a solution and conditions of at least 25° C. in 30 mM NaCl, 3 mM trisodium citrate, and 0.1% SDS over 30 min. Greater stringency may be obtained at 42° C. in 15 mM NaCl, with 1.5 mM trisodium citrate, and 0.1% SDS over 30 min. Even higher stringency wash conditions are obtained at 65° C.-68° C. in a solution of 15 mM NaCl, 1.5 mM trisodium citrate, and 0.1% SDS. Wash procedures will generally employ at least two final wash steps. Additional variations on these conditions will be readily apparent to those skilled in the art (see, for example, U.S. Patent Application No. 20010010913).

Stringency conditions can be selected such that an oligonucleotide that is perfectly complementary to the coding oligonucleotide hybridizes to the coding oligonucleotide with at least about a 5-10× higher signal to noise ratio than the ratio for hybridization of the perfectly complementary oligonucleotide to a nucleic acid encoding a transcription factor known as of the filing date of the application. It may be desirable to select conditions for a particular assay such that a higher signal to noise ratio, that is, about 15× or more, is obtained. Accordingly, a subject nucleic acid will hybridize to a unique coding oligonucleotide with at least a 2× or greater signal to noise ratio as compared to hybridization of the coding oligonucleotide to a nucleic acid encoding known polypeptide. The particular signal will depend on the label used in the relevant assay, e.g., a fluorescent label, a calorimetric label, a radioactive label, or the like. Labeled hybridization or PCR probes for detecting related polynucleotide sequences may be produced by oligolabeling, nick translation, end-labeling, or PCR amplification using a labeled nucleotide.

Identifying Polynucleotides or Nucleic Acids with Expression Libraries

In addition to hybridization methods, transcription factor homolog polypeptides can be obtained by screening an expression library using antibodies specific for one or more transcription factors. With the provision herein of the disclosed transcription factor, and transcription factor homolog nucleic acid sequences, the encoded polypeptide(s) can be expressed and purified in a heterologous expression system (e.g., E. coli) and used to raise antibodies (monoclonal or polyclonal) specific for the polypeptide(s) in question. Antibodies can also be raised against synthetic peptides derived from transcription factor, or transcription factor homolog, amino acid sequences. Methods of raising antibodies are well known in the art and are described in Harlow and Lane (1988), Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, New York. Such antibodies can then be used to screen an expression library produced from the plant from which it is desired to clone additional transcription factor homologs, using the methods described above. The selected cDNAs can be confirmed by sequencing and enzymatic activity.

Sequence Variations

It will readily be appreciated by those of skill in the art, that any of a variety of polynucleotide sequences are capable of encoding the transcription factors and transcription factor homolog polypeptides of the invention. Due to the degeneracy of the genetic code, many different polynucleotides can encode identical and/or substantially similar polypeptides in addition to those sequences illustrated in the Sequence Listing (except CBF polypeptide sequences SEQ ID NOs: 1956, 1958, 1960, or 2204). Nucleic acids having a sequence that differs from the sequences shown in the Sequence Listing, or complementary sequences, that encode functionally equivalent peptides (i.e., peptides having some degree of equivalent or similar biological activity) but differ in sequence from the sequence shown in the Sequence Listing due to degeneracy in the genetic code, are also within the scope of the invention.

Altered polynucleotide sequences encoding polypeptides include those sequences with deletions, insertions, or substitutions of different nucleotides, resulting in a polynucleotide encoding a polypeptide with at least one functional characteristic of the instant polypeptides. Included within this definition are polymorphisms which may or may not be readily detectable using a particular oligonucleotide probe of the polynucleotide encoding the instant polypeptides, and improper or unexpected hybridization to allelic variants, with a locus other than the normal chromosomal locus for the polynucleotide sequence encoding the instant polypeptides.

Allelic variant refers to any of two or more alternative forms of a gene occupying the same chromosomal locus. Allelic variation arises naturally through mutation, and may result in phenotypic polymorphism within populations. Gene mutations can be silent (i.e., no change in the encoded polypeptide) or may encode polypeptides having altered amino acid sequence. The term allelic variant is also used herein to denote a protein encoded by an allelic variant of a gene. Splice variant refers to alternative forms of RNA transcribed from a gene. Splice variation arises naturally through use of alternative splicing sites within a transcribed RNA molecule, or less commonly between separately transcribed RNA molecules, and may result in several mRNAs transcribed from the same gene. Splice variants may encode polypeptides having altered amino acid sequence. The term splice variant is also used herein to denote a protein encoded by a splice variant of an mRNA transcribed from a gene.

Those skilled in the art would recognize that, for example, G28, SEQ ID NO: 10, represents a single transcription factor; allelic variation and alternative splicing may be expected to occur. Allelic variants of SEQ ID NO: 9 can be cloned by probing cDNA or genomic libraries from different individual organisms according to standard procedures. Allelic variants of the DNA sequence shown in SEQ ID NO: 9, including those containing silent mutations and those in which mutations result in amino acid sequence changes, are within the scope of the present invention, as are proteins which are allelic variants of SEQ ID NO: 10. cDNAs generated from alternatively spliced mRNAs, which retain the properties of the transcription factor are included within the scope of the present invention, as are polypeptides encoded by such cDNAs and mRNAs. Allelic variants and splice variants of these sequences can be cloned by probing cDNA or genomic libraries from different individual organisms or tissues according to standard procedures known in the art (see U.S. Pat. No. 6,388,064).

Thus, in addition to the sequences set forth in the Sequence Listing (except CBF sequences), the invention also encompasses related nucleic acid molecules that include allelic or splice variants of SEQ ID NO: 2N−1, wherein N=1-229, SEQ ID NO: 459-466; 468-487; 491-500; 504; 506-511; 516-520; 523-524; 527; 529; 531-533; 538-539; 541-557; 560-568; 570-586; 595-596; 598-606; 610-620; 627-634; 640-664; 670-707; 714-719; 722-735; 740-741; 743-779; 808-823; 825-834; 838-850; 855-864; 868-889; 892-902; 908-909; 914-921; 924-925; 927-932; 935-942; 944-952; 961-965; 968-986; 989-993; 995-1010; 1012-1034; 1043-1063; 1074-1080; 1091-1104; 1111-1121; 1123-1128; 1134-1138; 1142-1156; 1159-1175; 1187-1190; 1192-1199; 1202-1220; 1249-1253; 1258-1262; 1264-1269; 1271-1287; 1292-1301; 1303-1309; 1315-1323; 1328-1337; 1340-1341; 1344-1361; 1365-1377; 1379-1390; 1393-1394; 1396-1398; 1419-1432; 1434-1452; 1455-1456; 1460-1465; 1468-1491; 1499; 1502; 1505-1521; 1523-1527; 1529-1532; 1536-1539; 1542-1562; 1567-1571; 1573-1582; 1587-1592; 1595-1620; 1625-1644; 1647-1654; 1659-1669; 1671-1673; 1675-1680; 1682-1686; 1688-1700; 1706-1709; 1714-1726; 1728-1734; 1738-1742; 1744-1753; 1757-1760; 1763-1764; 1766-1768; 1770-1780; 1782-1784; 1786-1789; 1791-1804; 1806-1812; 1814-1837; 1847-1856; 1858-1862; 1864-1873; 1876-1882; 1885-1896; 1902-1910; 1913-1916; 1921-1928; 1931-1936; 1940-1941; 1944-1946, or SEQ ID NO: 2N−1, wherein N=974-1101, and include sequences which are complementary to any of the above nucleotide sequences. Related nucleic acid molecules also include nucleotide sequences encoding a polypeptide comprising or consisting essentially of a substitution, modification, addition and/or deletion of one or more amino acid residues compared to the polypeptide as set forth in any of SEQ ID NO: 2N, wherein N=1-229, SEQ ID NO: 467; 488-490; 501-503; 505; 512-515; 521-522; 525-526; 528; 530; 534-537; 540; 558-559; 569; 587-594; 597; 607-609; 621-626; 635-639; 665-669; 708-713; 720-721; 736-739; 742; 780-807; 824; 835-837; 851-854; 865-867; 890-891; 903-907; 910-913; 922-923; 926; 933-934; 943; 953-960; 966-967; 987-988; 994; 1011; 1035-1042; 1064-1073; 1081-1090; 1105-1110; 1122; 1129-1133; 1139-1141; 1157-1158; 1176-1186; 1191; 1200-1201; 1221-1248; 1254-1257; 1263; 1270; 1288-1291; 1302; 1310-1314; 1324-1327; 1338-1339; 1342-1343; 1362-1364; 1378; 1391-1392; 1395; 1399-1418; 1433; 1453-1454; 1457-1459; 1466-1467; 1492-1498; 1500-1501; 1503-1504; 1522; 1528; 1533-1535; 1540-1541; 1563-1566; 1572; 1583-1586; 1593-1594; 1621-1624; 1645-1646; 1655-1658; 1670; 1674; 1681; 1687; 1701-1705; 1710-1713; 1727; 1735-1737; 1743; 1754-1756; 1761-1762; 1765; 1769; 1781; 1785; 1790; 1805; 1813; 1838-1846; 1857; 1863; 1874-1875; 1883-1884; 1897-1901; 1911-1912; 1917-1920; 1929-1930; 1937-1939; 1942-1943; or SEQ ID NO: 2N, wherein N=974-1101. Such related polypeptides may comprise, for example, additions and/or deletions of one or more N-linked or O-linked glycosylation sites, or an addition and/or a deletion of one or more cysteine residues.

For example, Table 1 illustrates, e.g., that the codons AGC, AGT, TCA, TCC, TCG, and TCT all encode the same amino acid: serine. Accordingly, at each position in the sequence where there is a codon encoding serine, any of the above trinucleotide sequences can be used without altering the encoded polypeptide.

TABLE 1 Amino acid Possible Codons Alanine Ala A GCA GCC GCG GCU Cysteine Cys C TGC TGT Aspartic acid Asp D GAC GAT Glutamic acid Glu E GAA GAG Phenylalanine Phe F TTC TTT Glycine Gly G GGA GGC GGG GGT Histidine His H CAC CAT Isoleucine Ile I ATA ATC ATT Lysine Lys K AAA AAG Leucine Leu L TTA TTG CTA CTC CTG CTT Methionine Met M ATG Asparagine Asn N AAC AAT Proline Pro P CCA CCC CCG CCT Glutamine Gln Q CAA CAG Arginine Arg R AGA AGG CGA CGC CGG CGT Serine Ser S AGC AGT TCA TCC TCG TCT Threonine Thr T ACA ACC ACG ACT Valine Val V GTA GTC GTG GTT Tryptophan Trp W TGG Tyrosine Tyr Y TAC TAT

Sequence alterations that do not change the amino acid sequence encoded by the polynucleotide are termed “silent” variations. With the exception of the codons ATG and TGG, encoding methionine and tryptophan, respectively, any of the possible codons for the same amino acid can be substituted by a variety of techniques, e.g., site-directed mutagenesis, available in the art. Accordingly, any and all such variations of a sequence selected from the above table are a feature of the invention.

In addition to silent variations, other conservative variations that alter one, or a few amino acids in the encoded polypeptide, can be made without altering the function of the polypeptide, these conservative variants are, likewise, a feature of the invention.

For example, substitutions, deletions and insertions introduced into the sequences provided in the Sequence Listing (except CBF polypeptide sequences SEQ ID NOs: 1956, 1958, 1960, or 2204, listed therein), are also envisioned by the invention. Such sequence modifications can be engineered into a sequence by site-directed mutagenesis (Wu (ed.) Methods Enzymol. (1993) vol. 217, Academic Press) or the other methods noted below. Amino acid substitutions are typically of single residues; insertions usually will be on the order of about from 1 to 10 amino acid residues; and deletions will range about from 1 to 30 residues. In preferred embodiments, deletions or insertions are made in adjacent pairs, e.g., a deletion of two residues or insertion of two residues. Substitutions, deletions, insertions or any combination thereof can be combined to arrive at a sequence. The mutations that are made in the polynucleotide encoding the transcription factor should not place the sequence out of reading frame and should not create complementary regions that could produce secondary mRNA structure. Preferably, the polypeptide encoded by the DNA performs the desired function.

Conservative substitutions are those in which at least one residue in the amino acid sequence has been removed and a different residue inserted in its place. Such substitutions generally are made in accordance with the Table 2 when it is desired to maintain the activity of the protein. Table 2 shows amino acids which can be substituted for an amino acid in a protein and which are typically regarded as conservative substitutions.

TABLE 2 Conservative Residue Substitutions Ala Ser Arg Lys Asn Gln; His Asp Glu Gln Asn Cys Ser Glu Asp Gly Pro His Asn; Gln Ile Leu, Val Leu Ile; Val Lys Arg; Gln Met Leu; Ile Phe Met; Leu; Tyr Ser Thr; Gly Thr Ser; Val Trp Tyr Tyr Trp; Phe Val Ile; Leu

Similar substitutions are those in which at least one residue in the amino acid sequence has been removed and a different residue inserted in its place. Such substitutions generally are made in accordance with the Table 3 when it is desired to maintain the activity of the protein. Table 3 shows amino acids which can be substituted for an amino acid in a protein and which are typically regarded as structural and functional substitutions. For example, a residue in column 1 of Table 3 may be substituted with a residue in column 2; in addition, a residue in column 2 of Table 3 may be substituted with the residue of column 1.

TABLE 3 Residue Similar Substitutions Ala Ser; Thr; Gly; Val; Leu; Ile Arg Lys; His; Gly Asn Gln; His; Gly; Ser; Thr Asp Glu, Ser; Thr Gln Asn; Ala Cys Ser; Gly Glu Asp Gly Pro; Arg His Asn; Gln; Tyr; Phe; Lys; Arg Ile Ala; Leu; Val; Gly; Met Leu Ala; Ile; Val; Gly; Met Lys Arg; His; Gln; Gly; Pro Met Leu; Ile; Phe Phe Met; Leu; Tyr; Trp; His; Val; Ala Ser Thr; Gly; Asp; Ala; Val; Ile; His Thr Ser; Val; Ala; Gly Trp Tyr; Phe; His Tyr Trp; Phe; His Val Ala; Ile; Leu; Gly; Thr; Ser; Glu

Substitutions that are less conservative than those in Table 2 can be selected by picking residues that differ more significantly in their effect on maintaining (a) the structure of the polypeptide backbone in the area of the substitution, for example, as a sheet or helical conformation, (b) the charge or hydrophobicity of the molecule at the target site, or (c) the bulk of the side chain. The substitutions which in general are expected to produce the greatest changes in protein properties will be those in which (a) a hydrophilic residue, e.g., seryl or threonyl, is substituted for (or by) a hydrophobic residue, e.g., leucyl, isoleucyl, phenylalanyl, valyl or alanyl; (b) a cysteine or proline is substituted for (or by) any other residue; (c) a residue having an electropositive side chain, e.g., lysyl, arginyl, or histidyl, is substituted for (or by) an electronegative residue, e.g., glutamyl or aspartyl; or (d) a residue having a bulky side chain, e.g., phenylalanine, is substituted for (or by) one not having a side chain, e.g., glycine.

Further Modifying Sequences of the Invention—Mutation/Forced Evolution

In addition to generating silent or conservative substitutions as noted, above, the present invention optionally includes methods of modifying the sequences of the Sequence Listing. In the methods, nucleic acid or protein modification methods are used to alter the given sequences to produce new sequences and/or to chemically or enzymatically modify given sequences to change the properties of the nucleic acids or proteins.

Thus, in one embodiment, given nucleic acid sequences are modified, e.g., according to standard mutagenesis or artificial evolution methods to produce modified sequences. The modified sequences may be created using purified natural polynucleotides isolated from any organism or may be synthesized from purified compositions and chemicals using chemical means well know to those of skill in the art. For example, Ausubel, supra, provides additional details on mutagenesis methods. Artificial forced evolution methods are described, for example, by Stemmer (1994) Nature 370: 389-391, Stemmer (1994) Proc. Natl. Acad. Sci. 91:10747-10751, and U.S. Pat. Nos. 5,811,238, 5,837,500, and 6,242,568. Methods for engineering synthetic transcription factors and other polypeptides are described, for example, by Zhang et al. (2000) J. Biol. Chem. 275: 33850-33860, Liu et al. (2001) J. Biol. Chem. 276: 11323-11334, and Isalan et al. (2001) Nature Biotechnol. 19: 656-660. Many other mutation and evolution methods are also available and expected to be within the skill of the practitioner.

Similarly, chemical or enzymatic alteration of expressed nucleic acids and polypeptides can be performed by standard methods. For example, sequence can be modified by addition of lipids, sugars, peptides, organic or inorganic compounds, by the inclusion of modified nucleotides or amino acids, or the like. For example, protein modification techniques are illustrated in Ausubel, supra. Further details on chemical and enzymatic modifications can be found herein. These modification methods can be used to modify any given sequence, or to modify any sequence produced by the various mutation and artificial evolution modification methods noted herein.

Accordingly, the invention provides for modification of any given nucleic acid by mutation, evolution, chemical or enzymatic modification, or other available methods, as well as for the products produced by practicing such methods, e.g., using the sequences herein as a starting substrate for the various modification approaches.

For example, optimized coding sequence containing codons preferred by a particular prokaryotic or eukaryotic host can be used e.g., to increase the rate of translation or to produce recombinant RNA transcripts having desirable properties, such as a longer half-life, as compared with transcripts produced using a non-optimized sequence. Translation stop codons can also be modified to reflect host preference. For example, preferred stop codons for Saccharomyces cerevisiae and mammals are TAA and TGA, respectively. The preferred stop codon for monocotyledonous plants is TGA, whereas insects and E. coli prefer to use TAA as the stop codon.

The polynucleotide sequences of the present invention can also be engineered in order to alter a coding sequence for a variety of reasons, including but not limited to, alterations which modify the sequence to facilitate cloning, processing and/or expression of the gene product. For example, alterations are optionally introduced using techniques which are well known in the art, e.g., site-directed mutagenesis, to insert new restriction sites, to alter glycosylation patterns, to change codon preference, to introduce splice sites, etc.

Furthermore, a fragment or domain derived from any of the polypeptides of the invention can be combined with domains derived from other transcription factors or synthetic domains to modify the biological activity of a transcription factor. For instance, a DNA-binding domain derived from a transcription factor of the invention can be combined with the activation domain of another transcription factor or with a synthetic activation domain. A transcription activation domain assists in initiating transcription from a DNA-binding site. Examples include the transcription activation region of VP16 or GAL4 (Moore et al. (1998) Proc. Natl. Acad. Sci. 95: 376-381; Aoyama et al. (1995) Plant Cell 7: 1773-1785), peptides derived from bacterial sequences (Ma and Ptashne (1987) Cell 51: 113-119) and synthetic peptides (Giniger and Ptashne (1987) Nature 330: 670-672).

Expression and Modification of Polypeptides

Typically, polynucleotide sequences of the invention are incorporated into recombinant DNA (or RNA) molecules that direct expression of polypeptides of the invention in appropriate host cells, transgenic plants, in vitro translation systems, or the like. Due to the inherent degeneracy of the genetic code, nucleic acid sequences which encode substantially the same or a functionally equivalent amino acid sequence can be substituted for any listed sequence to provide for cloning and expressing the relevant homolog.

The transgenic plants of the present invention comprising recombinant polynucleotide sequences are generally derived from parental plants, which may themselves be non-transformed (or non-transgenic) plants. These transgenic plants may either have a transcription factor gene “knocked out” (for example, with a genomic insertion by homologous recombination, an antisense or ribozyme construct) or expressed to a normal or wild-type extent. However, overexpressing transgenic “progeny” plants will exhibit greater mRNA levels, wherein the mRNA encodes a transcription factor, that is, a DNA-binding protein that is capable of binding to a DNA regulatory sequence and inducing transcription, and preferably, expression of a plant trait gene. Preferably, the mRNA expression level will be at least three-fold greater than that of the parental plant, or more preferably at least ten-fold greater mRNA levels compared to said parental plant, and most preferably at least fifty-fold greater compared to said parental plant.

Vectors, Promoters, and Expression Systems

The present invention includes recombinant constructs comprising one or more of the nucleic acid sequences herein. The constructs typically comprise a vector, such as a plasmid, a cosmid, a phage, a virus (e.g., a plant virus), a bacterial artificial chromosome (BAC), a yeast artificial chromosome (YAC), or the like, into which a nucleic acid sequence of the invention has been inserted, in a forward or reverse orientation. In a preferred aspect of this embodiment, the construct further comprises regulatory sequences, including, for example, a promoter, operably linked to the sequence. Large numbers of suitable vectors and promoters are known to those of skill in the art, and are commercially available.

General texts that describe molecular biological techniques useful herein, including the use and production of vectors, promoters and many other relevant topics, include Berger, Sambrook, supra and Ausubel, supra. Any of the identified sequences can be incorporated into a cassette or vector, e.g., for expression in plants. A number of expression vectors suitable for stable transformation of plant cells or for the establishment of transgenic plants have been described including those described in Weissbach and Weissbach (1989) Methods for Plant Molecular Biology, Academic Press, and Gelvin et al. (1990) Plant Molecular Biology Manual, Kluwer Academic Publishers. Specific examples include those derived from a Ti plasmid of Agrobacterium tumefaciens, as well as those disclosed by Herrera-Estrella et al. (1983) Nature 303: 209, Bevan (1984) Nucleic Acids Res. 12: 8711-8721, Klee (1985) Bio/Technology 3: 637-642, for dicotyledonous plants.

Alternatively, non-Ti vectors can be used to transfer the DNA into monocotyledonous plants and cells by using free DNA delivery techniques. Such methods can involve, for example, the use of liposomes, electroporation, microprojectile bombardment, silicon carbide whiskers, and viruses. By using these methods transgenic plants such as wheat, rice (Christou (1991) Bio/Technology 9: 957-962) and corn (Gordon-Kamm (1990) Plant Cell 2: 603-618) can be produced. An immature embryo can also be a good target tissue for monocots for direct DNA delivery techniques by using the particle gun (Weeks et al. (1993) Plant Physiol. 102: 1077-1084; Vasil (1993) Bio/Technology 10: 667-674; Wan and Lemeaux (1994) Plant Physiol. 104: 37-48, and for Agrobacterium-mediated DNA transfer (Ishida et al. (1996) Nature Biotechnol. 14: 745-750).

Typically, plant transformation vectors include one or more cloned plant coding sequence (genomic or cDNA) under the transcriptional control of 5′ and 3′ regulatory sequences and a dominant selectable marker. Such plant transformation vectors typically also contain a promoter (e.g., a regulatory region controlling inducible or constitutive, environmentally- or developmentally-regulated, or cell- or tissue-specific expression), a transcription initiation start site, an RNA processing signal (such as intron splice sites), a transcription termination site, and/or a polyadenylation signal.

A potential utility for the transcription factor polynucleotides disclosed herein is the isolation of promoter elements from these genes that can be used to program expression in plants of any genes. Each transcription factor gene disclosed herein is expressed in a unique fashion, as determined by promoter elements located upstream of the start of translation, and additionally within an intron of the transcription factor gene or downstream of the termination codon of the gene. As is well known in the art, for a significant portion of genes, the promoter sequences are located entirely in the region directly upstream of the start of translation. In such cases, typically the promoter sequences are located within 2.0 kb of the start of translation, or within 1.5 kb of the start of translation, frequently within 1.0 kb of the start of translation, and sometimes within 0.5 kb of the start of translation.

The promoter sequences can be isolated according to methods known to one skilled in the art.

Examples of constitutive plant promoters which can be useful for expressing the TF sequence include: the cauliflower mosaic virus (CaMV) 35S promoter, which confers constitutive, high-level expression in most plant tissues (see, e.g., Odell et al. (1985) Nature 313: 810-812); the nopaline synthase promoter (An et al. (1988) Plant Physiol. 88: 547-552); and the octopine synthase promoter (Fromm et al. (1989) Plant Cell 1: 977-984).

A variety of plant gene promoters that regulate gene expression in response to environmental, hormonal, chemical, developmental signals, and in a tissue-active manner can be used for expression of a TF sequence in plants. Choice of a promoter is based largely on the phenotype of interest and is determined by such factors as tissue (e.g., seed, fruit, root, pollen, vascular tissue, flower, carpel, etc.), inducibility (e.g., in response to wounding, heat, cold, drought, light, pathogens, etc.), timing, developmental stage, and the like. Numerous known promoters have been characterized and can favorably be employed to promote expression of a polynucleotide of the invention in a transgenic plant or cell of interest. For example, tissue specific promoters include: seed-specific promoters (such as the napin, phaseolin or DC3 promoter described in U.S. Pat. No. 5,773,697), fruit-specific promoters that are active during fruit ripening (such as the dru 1 promoter (U.S. Pat. No. 5,783,393), or the 2A11 promoter (U.S. Pat. No. 4,943,674) and the tomato polygalacturonase promoter (Bird et al. (1988) Plant Mol. Biol. 11: 651-662), root-specific promoters, such as those disclosed in U.S. Pat. Nos. 5,618,988, 5,837,848 and 5,905,186, pollen-active promoters such as PTA29, PTA26 and PTA13 (U.S. Pat. No. 5,792,929), promoters active in vascular tissue (Ringli and Keller (1998) Plant Mol. Biol. 37: 977-988), flower-specific (Kaiser et al. (1995) Plant Mol. Biol. 28: 231-243), pollen (Baerson et al. (1994) Plant Mol. Biol. 26: 1947-1959), carpels (Ohl et al. (1990) Plant Cell 2: 837-848), pollen and ovules (Baerson et al. (1993) Plant Mol. Biol. 22: 255-267), auxin-inducible promoters (such as that described in van der Kop et al. (1999) Plant Mol. Biol. 39: 979-990 or Baumann et al. (1999) Plant Cell 11: 323-334), cytokinin-inducible promoter (Guevara-Garcia (1998) Plant Mol. Biol. 38: 743-753), promoters responsive to gibberellin (Shi et al. (1998) Plant Mol. Biol. 38: 1053-1060, Willmott et al. (1998) 38: 817-825) and the like. Additional promoters are those that elicit expression in response to heat (Ainley et al. (1993) Plant Mol. Biol. 22: 13-23), light (e.g., the pea rbcS-3A promoter, Kuhlemeier et al. (1989) Plant Cell 1: 471-478, and the maize rbcS promoter, Schaffner and Sheen (1991) Plant Cell 3: 997-1012); wounding (e.g., wunI, Siebertz et al. (1989) Plant Cell 1: 961-968); pathogens (such as the PR-1 promoter described in Buchel et al. (1999) Plant Mol. Biol. 40: 387-396, and the PDF1.2 promoter described in Manners et al. (1998) Plant Mol. Biol. 38: 1071-1080), and chemicals such as methyl jasmonate or salicylic acid (Gatz (1997) Annu. Rev. Plant Physiol. Plant Mol. Biol. 48: 89-108). In addition, the timing of the expression can be controlled by using promoters such as those acting at senescence (Gan and Amasino (1995) Science 270: 1986-1988); or late seed development (Odell et al. (1994) Plant Physiol. 106: 447-458).

Plant expression vectors can also include RNA processing signals that can be positioned within, upstream or downstream of the coding sequence. In addition, the expression vectors can include additional regulatory sequences from the 3′-untranslated region of plant genes, e.g., a 3′ terminator region to increase mRNA stability of the mRNA, such as the PI-II terminator region of potato or the octopine or nopaline synthase 3′ terminator regions.

Additional Expression Elements

Specific initiation signals can aid in efficient translation of coding sequences. These signals can include, e.g., the ATG initiation codon and adjacent sequences. In cases where a coding sequence, its initiation codon and upstream sequences are inserted into the appropriate expression vector, no additional translational control signals may be needed. However, in cases where only coding sequence (e.g., a mature protein coding sequence), or a portion thereof, is inserted, exogenous transcriptional control signals including the ATG initiation codon can be separately provided. The initiation codon is provided in the correct reading frame to facilitate transcription. Exogenous transcriptional elements and initiation codons can be of various origins, both natural and synthetic. The efficiency of expression can be enhanced by the inclusion of enhancers appropriate to the cell system in use.

Expression Hosts

The present invention also relates to host cells which are transduced with vectors of the invention, and the production of polypeptides of the invention (including fragments thereof) by recombinant techniques. Host cells are genetically engineered (i.e., nucleic acids are introduced, e.g., transduced, transformed or transfected) with the vectors of this invention, which may be, for example, a cloning vector or an expression vector comprising the relevant nucleic acids herein. The vector is optionally a plasmid, a viral particle, a phage, a naked nucleic acid, etc. The engineered host cells can be cultured in conventional nutrient media modified as appropriate for activating promoters, selecting transformants, or amplifying the relevant gene. The culture conditions, such as temperature, pH and the like, are those previously used with the host cell selected for expression, and will be apparent to those skilled in the art and in the references cited herein, including, Sambrook, supra and Ausubel, supra.

The host cell can be a eukaryotic cell, such as a yeast cell, or a plant cell, or the host cell can be a prokaryotic cell, such as a bacterial cell. Plant protoplasts are also suitable for some applications. For example, the DNA fragments are introduced into plant tissues, cultured plant cells or plant protoplasts by standard methods including electroporation (Fromm et al. (1985) Proc. Natl. Acad. Sci. 82: 5824-5828, infection by viral vectors such as cauliflower mosaic virus (CaMV) (Hohn et al. (1982) Molecular Biology of Plant Tumors Academic Press, New York, N.Y., pp. 549-560; U.S. Pat. No. 4,407,956), high velocity ballistic penetration by small particles with the nucleic acid either within the matrix of small beads or particles, or on the surface (Klein et al. (1987) Nature 327: 70-73), use of pollen as vector (WO 85/01856), or use of Agrobacterium tumefaciens or A. rhizogenes carrying a T-DNA plasmid in which DNA fragments are cloned. The T-DNA plasmid is transmitted to plant cells upon infection by Agrobacterium tumefaciens, and a portion is stably integrated into the plant genome (Horsch et al. (1984) Science 233: 496-498; Fraley et al. (1983) Proc. Natl. Acad. Sci. 80: 4803-4807).

The cell can include a nucleic acid of the invention that encodes a polypeptide, wherein the cell expresses a polypeptide of the invention. The cell can also include vector sequences, or the like. Furthermore, cells and transgenic plants that include any polypeptide or nucleic acid above or throughout this specification, e.g., produced by transduction of a vector of the invention, are an additional feature of the invention.

For long-term, high-yield production of recombinant proteins, stable expression can be used. Host cells transformed with a nucleotide sequence encoding a polypeptide of the invention are optionally cultured under conditions suitable for the expression and recovery of the encoded protein from cell culture. The protein or fragment thereof produced by a recombinant cell may be secreted, membrane-bound, or contained intracellularly, depending on the sequence and/or the vector used. As will be understood by those of skill in the art, expression vectors containing polynucleotides encoding mature proteins of the invention can be designed with signal sequences which direct secretion of the mature polypeptides through a prokaryotic or eukaryotic cell membrane.

Modified Amino Acid Residues

Polypeptides of the invention may contain one or more modified amino acid residues. The presence of modified amino acids may be advantageous in, for example, increasing polypeptide half-life, reducing polypeptide antigenicity or toxicity, increasing polypeptide storage stability, or the like. Amino acid residue(s) are modified, for example, co-translationally or post-translationally during recombinant production or modified by synthetic or chemical means.

Non-limiting examples of a modified amino acid residue include incorporation or other use of acetylated amino acids, glycosylated amino acids, sulfated amino acids, prenylated (e.g., farnesylated, geranylgeranylated) amino acids, PEG modified (e.g., “PEGylated”) amino acids, biotinylated amino acids, carboxylated amino acids, phosphorylated amino acids, etc. References adequate to guide one of skill in the modification of amino acid residues are replete throughout the literature.

The modified amino acid residues may prevent or increase affinity of the polypeptide for another molecule, including, but not limited to, polynucleotide, proteins, carbohydrates, lipids and lipid derivatives, and other organic or synthetic compounds.

Identification of Additional Factors

A transcription factor provided by the present invention can also be used to identify additional endogenous or exogenous molecules that can affect a phenotype or trait of interest. On the one hand, such molecules include organic (small or large molecules) and/or inorganic compounds that affect expression of (i.e., regulate) a particular transcription factor. Alternatively, such molecules include endogenous molecules that are acted upon either at a transcriptional level by a transcription factor of the invention to modify a phenotype as desired. For example, the transcription factors can be employed to identify one or more downstream genes that are subject to a regulatory effect of the transcription factor. In one approach, a transcription factor or transcription factor homolog of the invention is expressed in a host cell, e.g., a transgenic plant cell, tissue or explant, and expression products, either RNA or protein, of likely or random targets are monitored, e.g., by hybridization to a microarray of nucleic acid probes corresponding to genes expressed in a tissue or cell type of interest, by two-dimensional gel electrophoresis of protein products, or by any other method known in the art for assessing expression of gene products at the level of RNA or protein. Alternatively, a transcription factor of the invention can be used to identify promoter sequences (such as binding sites on DNA sequences) involved in the regulation of a downstream target. After identifying a promoter sequence, interactions between the transcription factor and the promoter sequence can be modified by changing specific nucleotides in the promoter sequence or specific amino acids in the transcription factor that interact with the promoter sequence to alter a plant trait. Typically, transcription factor DNA-binding sites are identified by gel shift assays. After identifying the promoter regions, the promoter region sequences can be employed in double-stranded DNA arrays to identify molecules that affect the interactions of the transcription factors with their promoters (Bulyk et al. (1999) Nature Biotechnol. 17: 573-577).

The identified transcription factors are also useful to identify proteins that modify the activity of the transcription factor. Such modification can occur by covalent modification, such as by phosphorylation, or by protein-protein (homo or -heteropolymer) interactions. Any method suitable for detecting protein-protein interactions can be employed. Among the methods that can be employed are co-immunoprecipitation, cross-linking and co-purification through gradients or chromatographic columns, and the two-hybrid yeast system.

The two-hybrid system detects protein interactions in vivo and is described in Chien et al. (1991) Proc. Natl. Acad. Sci. 88: 9578-9582, and is commercially available from Clontech (Palo Alto, Calif.). In such a system, plasmids are constructed that encode two hybrid proteins: one consists of the DNA-binding domain of a transcription activator protein fused to the TF polypeptide and the other consists of the transcription activator protein's activation domain fused to an unknown protein that is encoded by a cDNA that has been recombined into the plasmid as part of a cDNA library. The DNA-binding domain fusion plasmid and the cDNA library are transformed into a strain of the yeast Saccharomyces cerevisiae that contains a reporter gene (e.g., lacZ) whose regulatory region contains the transcription activator's binding site. Either hybrid protein alone cannot activate transcription of the reporter gene. Interaction of the two hybrid proteins reconstitutes the functional activator protein and results in expression of the reporter gene, which is detected by an assay for the reporter gene product. Then, the library plasmids responsible for reporter gene expression are isolated and sequenced to identify the proteins encoded by the library plasmids. After identifying proteins that interact with the transcription factors, assays for compounds that interfere with the TF protein-protein interactions can be preformed.

Identification of Modulators

In addition to the intracellular molecules described above, extracellular molecules that alter activity or expression of a transcription factor, either directly or indirectly, can be identified. For example, the methods can entail first placing a candidate molecule in contact with a plant or plant cell. The molecule can be introduced by topical administration, such as spraying or soaking of a plant, or incubating a plant in a solution containing the molecule, and then the molecule's effect on the expression or activity of the TF polypeptide or the expression of the polynucleotide monitored. Changes in the expression of the TF polypeptide can be monitored by use of polyclonal or monoclonal antibodies, gel electrophoresis or the like. Changes in the expression of the corresponding polynucleotide sequence can be detected by use of microarrays, Northerns, quantitative PCR, or any other technique for monitoring changes in mRNA expression. These techniques are exemplified in Ausubel et al. (eds.) Current Protocols in Molecular Biology, John Wiley & Sons (1998, and supplements through 2001). Changes in the activity of the transcription factor can be monitored, directly or indirectly, by assaying the function of the transcription factor, for example, by measuring the expression of promoters known to be controlled by the transcription factor (using promoter-reporter constructs), measuring the levels of transcripts using microarrays, Northern blots, quantitative PCR, etc. Such changes in the expression levels can be correlated with modified plant traits and thus identified molecules can be useful for soaking or spraying on fruit, vegetable and grain crops to modify traits in plants.

Essentially any available composition can be tested for modulatory activity of expression or activity of any nucleic acid or polypeptide herein. Thus, available libraries of compounds such as chemicals, polypeptides, nucleic acids and the like can be tested for modulatory activity. Often, potential modulator compounds can be dissolved in aqueous or organic (e.g., DMSO-based) solutions for easy delivery to the cell or plant of interest in which the activity of the modulator is to be tested. Optionally, the assays are designed to screen large modulator composition libraries by automating the assay steps and providing compounds from any convenient source to assays, which are typically run in parallel (e.g., in microtiter formats on microplates in robotic assays).

In one embodiment, high throughput screening methods involve providing a combinatorial library containing a large number of potential compounds (potential modulator compounds). Such “combinatorial chemical libraries” are then screened in one or more assays, as described herein, to identify those library members (particular chemical species or subclasses) that display a desired characteristic activity. The compounds thus identified can serve as target compounds.

A combinatorial chemical library can be, e.g., a collection of diverse chemical compounds generated by chemical synthesis or biological synthesis. For example, a combinatorial chemical library such as a polypeptide library is formed by combining a set of chemical building blocks (e.g., in one example, amino acids) in every possible way for a given compound length (i.e., the number of amino acids in a polypeptide compound of a set length). Exemplary libraries include peptide libraries, nucleic acid libraries, antibody libraries (see, e.g., Vaughn et al. (1996) Nature Biotechnol. 14: 309-314 and PCT/US96/10287), carbohydrate libraries (see, e.g., Liang et al. Science (1996) 274: 1520-1522 and U.S. Pat. No. 5,593,853), peptide nucleic acid libraries (see, e.g., U.S. Pat. No. 5,539,083), and small organic molecule libraries (see, e.g., benzodiazepines, in Baum Chem. & Engineering News Jan. 18, 1993, page 33; isoprenoids, U.S. Pat. No. 5,569,588; thiazolidinones and metathiazanones, U.S. Pat. No. 5,549,974; pyrrolidines, U.S. Pat. Nos. 5,525,735 and 5,519,134; morpholino compounds, U.S. Pat. No. 5,506,337) and the like.

Preparation and screening of combinatorial or other libraries is well known to those of skill in the art. Such combinatorial chemical libraries include, but are not limited to, peptide libraries (see, e.g., U.S. Pat. No. 5,010,175; Furka, (1991) Int. J. Pept. Prot. Res. 37: 487-493; and Houghton et al. (1991) Nature 354: 84-88). Other chemistries for generating chemical diversity libraries can also be used.

In addition, as noted, compound screening equipment for high-throughput screening is generally available, e.g., using any of a number of well known robotic systems that have also been developed for solution phase chemistries useful in assay systems. These systems include automated workstations including an automated synthesis apparatus and robotic systems utilizing robotic arms. Any of the above devices are suitable for use with the present invention, e.g., for high-throughput screening of potential modulators. The nature and implementation of modifications to these devices (if any) so that they can operate as discussed herein will be apparent to persons skilled in the relevant art.

Indeed, entire high-throughput screening systems are commercially available. These systems typically automate entire procedures including all sample and reagent pipetting, liquid dispensing, timed incubations, and final readings of the microplate in detector(s) appropriate for the assay. These configurable systems provide high throughput and rapid start up as well as a high degree of flexibility and customization. Similarly, microfluidic implementations of screening are also commercially available.

The manufacturers of such systems provide detailed protocols the various high throughput. Thus, for example, Zymark Corp. provides technical bulletins describing screening systems for detecting the modulation of gene transcription, ligand binding, and the like. The integrated systems herein, in addition to providing for sequence alignment and, optionally, synthesis of relevant nucleic acids, can include such screening apparatus to identify modulators that have an effect on one or more polynucleotides or polypeptides according to the present invention.

In some assays it is desirable to have positive controls to ensure that the components of the assays are working properly. At least two types of positive controls are appropriate. That is, known transcriptional activators or inhibitors can be incubated with cells or plants, for example, in one sample of the assay, and the resulting increase/decrease in transcription can be detected by measuring the resulting increase in RNA levels and/or protein expression, for example, according to the methods herein. It will be appreciated that modulators can also be combined with transcriptional activators or inhibitors to find modulators that inhibit transcriptional activation or transcriptional repression. Either expression of the nucleic acids and proteins herein or any additional nucleic acids or proteins activated by the nucleic acids or proteins herein, or both, can be monitored.

In an embodiment, the invention provides a method for identifying compositions that modulate the activity or expression of a polynucleotide or polypeptide of the invention. For example, a test compound, whether a small or large molecule, is placed in contact with a cell, plant (or plant tissue or explant), or composition comprising the polynucleotide or polypeptide of interest and a resulting effect on the cell, plant, (or tissue or explant) or composition is evaluated by monitoring, either directly or indirectly, one or more of: expression level of the polynucleotide or polypeptide, activity (or modulation of the activity) of the polynucleotide or polypeptide. In some cases, an alteration in a plant phenotype can be detected following contact of a plant (or plant cell, or tissue or explant) with the putative modulator, e.g., by modulation of expression or activity of a polynucleotide or polypeptide of the invention. Modulation of expression or activity of a polynucleotide or polypeptide of the invention may also be caused by molecular elements in a signal transduction second messenger pathway and such modulation can affect similar elements in the same or another signal transduction second messenger pathway.

Subsequences

Also contemplated are uses of polynucleotides, also referred to herein as oligonucleotides, typically having at least 12 bases, preferably at least 15, more preferably at least 20, 30, or 50 bases, which hybridize under at least highly stringent (or ultra-high stringent or ultra-ultra-high stringent conditions) conditions to a polynucleotide sequence described above. The polynucleotides may be used as probes, primers, sense and antisense agents, and the like, according to methods as noted supra.

Subsequences of the polynucleotides of the invention, including polynucleotide fragments and oligonucleotides are useful as nucleic acid probes and primers. An oligonucleotide suitable for use as a probe or primer is at least about 15 nucleotides in length, more often at least about 18 nucleotides, often at least about 21 nucleotides, frequently at least about 30 nucleotides, or about 40 nucleotides, or more in length. A nucleic acid probe is useful in hybridization protocols, e.g., to identify additional polypeptide homologs of the invention, including protocols for microarray experiments. Primers can be annealed to a complementary target DNA strand by nucleic acid hybridization to form a hybrid between the primer and the target DNA strand, and then extended along the target DNA strand by a DNA polymerase enzyme. Primer pairs can be used for amplification of a nucleic acid sequence, e.g., by the polymerase chain reaction (PCR) or other nucleic-acid amplification methods. See Sambrook, supra, and Ausubel, supra.

In addition, the invention includes an isolated or recombinant polypeptide including a subsequence of at least about 15 contiguous amino acids encoded by the recombinant or isolated polynucleotides of the invention. For example, such polypeptides, or domains or fragments thereof, can be used as immunogens, e.g., to produce antibodies specific for the polypeptide sequence, or as probes for detecting a sequence of interest. A subsequence can range in size from about 15 amino acids in length up to and including the full length of the polypeptide.

To be encompassed by the present invention, an expressed polypeptide which comprises such a polypeptide subsequence performs at least one biological function of the intact polypeptide in substantially the same manner, or to a similar extent, as does the intact polypeptide. For example, a polypeptide fragment can comprise a recognizable structural motif or functional domain such as a DNA binding domain that activates transcription, e.g., by binding to a specific DNA promoter region an activation domain, or a domain for protein-protein interactions.

Production of Transgenic Plants

Modification of Traits

The polynucleotides of the invention are favorably employed to produce transgenic plants with various traits, or characteristics, that have been modified in a desirable manner, e.g., to improve the seed characteristics of a plant. For example, alteration of expression levels or patterns (e.g., spatial or temporal expression patterns) of one or more of the transcription factors (or transcription factor homologs) of the invention, as compared with the levels of the same protein found in a wild-type plant, can be used to modify a plant's traits. An illustrative example of trait modification, improved characteristics, by altering expression levels of a particular transcription factor is described further in the Examples and the Sequence Listing.

Arabidopsis as a Model System

Arabidopsis thaliana is the object of rapidly growing attention as a model for genetics and metabolism in plants. Arabidopsis has a small genome, and well-documented studies are available. It is easy to grow in large numbers and mutants defining important genetically controlled mechanisms are either available, or can readily be obtained. Various methods to introduce and express isolated homologous genes are available (see Koncz et al. eds., et al. Methods in Arabidopsis Research (1992) et al. World Scientific, New Jersey, N.J., in “Preface”). Because of its small size, short life cycle, obligate autogamy and high fertility, Arabidopsis is also a choice organism for the isolation of mutants and studies in morphogenetic and development pathways, and control of these pathways by transcription factors (Koncz supra, p. 72). A number of studies introducing transcription factors into A. thaliana have demonstrated the utility of this plant for understanding the mechanisms of gene regulation and trait alteration in plants. (See, for example, Koncz supra, and U.S. Pat. No. 6,417,428).

Arabidopsis Genes in Transgenic Plants.

Expression of genes which encode transcription factors modify expression of endogenous genes, polynucleotides, and proteins are well known in the art. In addition, transgenic plants comprising isolated polynucleotides encoding transcription factors may also modify expression of endogenous genes, polynucleotides, and proteins. Examples include Peng et al. (1997) et al. Genes and Development 11: 3194-3205, and Peng et al. (1999) Nature 400: 256-261. In addition, many others have demonstrated that an Arabidopsis transcription factor expressed in an exogenous plant species elicits the same or very similar phenotypic response. See, for example, Fu et al. (2001) Plant Cell 13: 1791-1802; Nandi et al. (2000) Curr. Biol. 10: 215-218; Coupland (1995) Nature 377: 482-483; and Weigel and Nilsson (1995) Nature 377: 482-500.

Homologous Genes Introduced into Transgenic Plants.

Homologous genes that may be derived from any plant, or from any source whether natural, synthetic, semi-synthetic or recombinant, and that share significant sequence identity or similarity to those provided by the present invention, may be introduced into plants, for example, crop plants, to confer desirable or improved traits. Consequently, transgenic plants may be produced that comprise a recombinant expression vector or cassette with a promoter operably linked to one or more sequences homologous to presently disclosed sequences. The promoter may be, for example, a plant or viral promoter.

The invention thus provides for methods for preparing transgenic plants, and for modifying plant traits. These methods include introducing into a plant a recombinant expression vector or cassette comprising a functional promoter operably linked to one or more sequences homologous to presently disclosed sequences. Plants and kits for producing these plants that result from the application of these methods are also encompassed by the present invention.

Transcription Factors of Interest for the Modification of Plant Traits

Currently, the existence of a series of maturity groups for different latitudes represents a major barrier to the introduction of new valuable traits. Any trait (e.g. disease resistance) has to be bred into each of the different maturity groups separately, a laborious and costly exercise. The availability of single strain, which could be grown at any latitude, would therefore greatly increase the potential for introducing new traits to crop species such as soybean and cotton.

For many of the specific effects, traits and utilities listed in Table 4 and Table 6 that may be conferred to plants, one or more transcription factor genes may be used to increase or decrease, advance or delay, or improve or prove deleterious to a given trait. Overexpressing or suppressing one or more genes can impart significant differences in production of plant products, such as different fatty acid ratios. For example, overexpression of G720 caused a plant to become more freezing tolerant, but knocking out the same transcription factor imparted greater susceptibility to freezing. Thus, suppressing a gene that causes a plant to be more sensitive to cold may improve a plant's tolerance of cold. More than one transcription factor gene may be introduced into a plant, either by transforming the plant with one or more vectors comprising two or more transcription factors, or by selective breeding of plants to yield hybrid crosses that comprise more than one introduced transcription factor.

A listing of specific effects and utilities that the presently disclosed transcription factor genes have on plants, as determined by direct observation and assay analysis, is provided in Table 4. Table 4 shows the polynucleotides identified by SEQ ID NO; Mendel Gene ID No. (GID); and if the polynucleotide was tested in a transgenic assay. The first column shows the polynucleotide SEQ ID NO; the second column shows the GID; the third column shows whether the gene was overexpressed (OE) or knocked out (KO) in plant studies; the fourth column shows the trait(s) resulting from the knock out or overexpression of the polynucleotide in the transgenic plant; the fifth column shows the category of the trait; and the sixth column (“Comment”), includes specific observations made with respect to the polynucleotide of the first column.

TABLE 4 Traits, trait categories, and effects and utilities that transcription factor genes have on plants. Polynucleotide GID OE/ SEQ ID NO: No. KO Trait(s) Category Observations 1 G8 OE Flowering time Flowering time Late flowering 3 G19 OE Erysiphe Disease Increased tolerance to Erysiphe; repressed by methyl jasmonate and induced by 1-aminocyclopropane 1- carboxylic acid (ACC) 5 G22 OE Sodium chloride Abiotic stress Increased tolerance to high salt 7 G24 OE Morphology: other Dev and morph Reduced size and necrotic patches 9 G28 OE Botrytis Disease Increased tolerance to Botrytis Sclerotinia Disease Increased tolerance to Sclerotinia Erysiphe Disease Increased resistance to Erysiphe 11 G47 OE Stem Dev and morph Altered structure of vascular tissues Osmotic Abiotic stress Better root growth under osmotic Flowering time Flowering time stress Architecture Dev and morph Late flowering Architecture Dev and morph Altered architecture and inflorescence development Reduced apical dominance 13 G156 KO Seed Dev and morph Seed color alteration 15 G157 OE Flowering time Flowering time Altered flowering time (modest level of overexpression triggers early flowering, whereas a larger increase delays flowering) 17 G162 OE Seed oil content Seed biochemistry Increased seed oil content Seed protein content Seed biochemistry Increased seed protein content 19 G175 OE Osmotic Abiotic stress Increased tolerance to osmotic stress 21 G180 OE Seed oil content Seed biochemistry Decreased seed oil Flowering time Flowering time Early flowering 23 G183 OE Flowering time Flowering time Early flowering Light response Dev and morph Constitutive photomorphogenesis 25 G188 KO Fusarium Disease Increased susceptibility to Fusarium Osmotic Abiotic stress Better germination under osmotic stress 27 G189 OE Size Dev and morph Increased leaf size 29 G192 OE Flowering time Flowering time Late flowering Seed oil content Seed biochemistry Decreased seed oil content 31 G196 OE Sodium chloride Abiotic stress Increased tolerance to high salt 33 G211 OE Leaf insoluble sugars Leaf biochemistry Increase in leaf xylose Architecture Dev and morph Reduced apical dominance Leaf Dev and morph Altered leaf shape 35 G214 OE Flowering time Flowering time Late flowering Leaf fatty acids Leaf biochemistry Increased leaf fatty acids Seed prenyl lipids Seed biochemistry Increased seed lutein Leaf prenyl lipids Leaf biochemistry Increased leaf chlorophyll and carotenoids 37 G226 OE Seed protein content Seed biochemistry Increased seed protein Trichome Dev and morph Glabrous, lack of trichomes Root Dev and morph Increased root hairs Sodium chloride Abiotic stress Increased tolerance to high salt Nutrient uptake Abiotic stress Increased tolerance to nitrogen- limited medium 39 G241 KO Seed protein content Seed biochemistry Increased seed protein content Seed oil content Seed biochemistry Decreased seed oil Sugar sensing Sugar sensing Decreased germination and growth on glucose medium 41 G248 OE Botrytis Disease Increased susceptibility to Botrytis 43 G254 OE Sugar sensing Sugar sensing Decreased germination and growth on glucose medium 45 G256 OE Cold, chilling Abiotic stress Better germination and growth in cold 47 G278 OE Sclerotinia Disease Increased susceptibility to Sclerotinia 49 G291 OE Seed oil content Seed biochemistry Increased seed oil content 51 G303 OE Osmotic Abiotic stress Better germination on high sucrose and high NaCl 53 G312 OE Sodium chloride Abiotic stress Better germination on high NaCl 55 G325 OE Osmotic Abiotic stress Better germination on high sucrose and NaCl 57 G343 OE Glyphosate Herbicide sensitivity Increased resistance to glyphosate Size Dev and morph Small plant 59 G353 OE Osmotic Abiotic stress Increased seedling vigor on polyethylene glycol (PEG) Size Dev and morph Reduced size Leaf Dev and morph Altered leaf development Flower Dev and morph Short pedicels, downward pointing siliques 61 G354 OE Size Dev and morph Reduced size Light response Dev and morph Constitutive photomorphogenesis Flower Dev and morph Short pedicels, downward pointing siliques 63 G361 OE Flowering time Flowering time Late flowering 65 G362 OE Flowering time Flowering time Late flowering Size Dev and morph Reduced size Trichome Dev and morph Ectopic trichome formation, increased trichome number Morphology: other Dev and morph Increased pigmentation in seed and embryos, and in other organs 67 G371 OE Botrytis Disease Increased susceptibility to Botrytis 69 G390 OE Architecture Dev and morph Altered shoot development 71 G391 OE Architecture Dev and morph Altered shoot development 73 G409 OE Erysiphe Disease Increased tolerance to Erysiphe 75 G427 OE Seed oil content Seed biochemistry Increased oil content Seed protein content Seed biochemistry Decreased protein content 77 G438 KO Stem Dev and morph Reduced lignin Architecture Dev and morph Reduced branching 79 G450 OE Seed Dev and morph Increased seed size 81 G464 OE Heat Abiotic stress Better germination and growth in heat 83 G470 OE Fertility Dev and morph Short stamen filaments 85 G477 OE Sclerotinia Disease Increased susceptibility to Oxidative Abiotic stress Sclerotinia Increased sensitivity to oxidative stress 87 G481 OE Sugar sensing Sugar sensing Better germination on sucrose media Drought Abiotic stress Increased tolerance to drought 89 G482 OE Sodium chloride Abiotic stress Increased tolerance to high salt 91 G484 KO Seed glucosinolates Seed biochemistry Altered glucosinolate profile 93 G489 OE Osmotic Abiotic stress Increased tolerance to osmotic stress 95 G490 OE Flowering time Flowering time Early flowering 97 G504 OE Seed oil composition Seed biochemistry Decreased seed oil composition and content; increase in 18:2 fatty acid and decrease in 20:1 fatty acid 99 G509 KO Seed oil content Seed biochemistry Increased total seed oil and protein Seed protein content Seed biochemistry content 101 G519 OE Seed oil content Seed biochemistry Increased seed oil content 103 G545 OE Sodium chloride Abiotic stress Susceptible to high salt Erysiphe Disease Increased susceptibility to Erysiphe Pseudomonas Disease Increased susceptibility to Fusarium Disease Pseudomonas Nutrient uptake Abiotic stress Increased susceptibility to Fusarium Increased tolerance to phosphate-free medium 105 G546 OE Hormone sensitivity Hormone sensitivity Decreased sensitivity to abscisic acid (ABA) 107 G561 OE Seed oil content Seed biochemistry Increased seed oil content Nutrient uptake Abiotic stress Increased tolerance to potassium-free medium 109 G562 OE Flowering time Flowering time Late flowering 111 G567 OE Seed oil content Seed biochemistry Increased total seed oil/protein Seed protein content Seed biochemistry content Sugar sensing Sugar sensing Increased total seed oil/protein content Decreased seedling vigor on high glucose 113 G568 OE Architecture Dev and morph Altered branching 115 G584 OE Seed Dev and morph Large seeds 117 G585 OE Trichome Dev and morph Reduced trichome density 119 G590 KO Seed oil content Seed biochemistry Increased seed oil content OE Flowering time Flowering time Early flowering 121 G594 OE Sclerotinia Disease Increased susceptibility to Sclerotinia 123 G597 OE Seed protein content Seed biochemistry Altered seed protein content 125 G598 OE Seed oil content Seed biochemistry Increased seed oil 127 G634 OE Trichome Dev and morph Increased trichome density and size 129 G635 OE Variegation Dev and morph Altered coloration 131 G636 OE Senescence Dev and morph Premature senescence 133 G638 OE Flower Dev and morph Altered flower development 135 G652 KO Seed prenyl lipids Seed biochemistry Increase in alpha-tocopherol 137 G663 OE Biochemistry: other Biochem: misc Increased anthocyanins in leaf, root, seed 139 G664 OE Cold, chilling Abiotic stress Better germination and growth in cold 141 G674 OE Leaf Dev and morph Dark green, upwardly oriented leaves 143 G676 OE Trichome Dev and morph Reduced trichome number, ectopic trichome formation 145 G680 OE Sugar sensing Sugar sensing Reduced germination on glucose medium 147 G682 OE Trichome Dev and morph Glabrous, lack of trichomes Heat Abiotic stress Better germination and growth in Root Dev and morph heat Increased root hairs 149 G715 OE Seed oil content Seed biochemistry Increased seed oil content 151 G720 OE Freezing Abiotic stress More freezing tolerant KO Freezing Abiotic stress Increased susceptibility to freezing 153 G736 OE Flowering time Flowering time Late flowering Leaf Dev and morph Altered leaf shape 155 G748 OE Seed prenyl lipids Seed biochemistry Increased lutein content Stem Dev and morph More vascular bundles in stem Flowering time Flowering time Late flowering 157 G779 OE Fertility Dev and morph Reduced fertility Flower Dev and morph Homeotic transformations 159 G789 OE Flowering time Flowering time Early flowering 161 G801 OE Sodium chloride Abiotic stress Better germination on high NaCl 163 G849 KO Seed oil content Seed biochemistry Increased seed oil content Seed protein content Seed biochemistry Altered seed protein content 165 G859 OE Flowering time Flowering time Late flowering 167 G864 OE Heat Abiotic stress Better germination in heat 169 G867 OE Sodium chloride Abiotic stress Better seedling vigor on high salt Sugar sensing Sugar sensing Better seedling vigor on high sucrose 171 G869 OE Seed oil composition Seed biochemistry Altered seed fatty acids 173 G877 KO Embryo lethal Dev and morph Embryo lethal phenotype: potential herbicide target 175 G881 OE Erysiphe Disease Increased susceptibility to Erysiphe 177 G892 KO Seed protein content Seed biochemistry Altered seed protein content Seed oil content Seed biochemistry Altered seed oil content 179 G896 KO Fusarium Disease Increased susceptibility to Fusarium 181 G910 OE Flowering time Flowering time Late flowering 183 G911 OE Nutrient uptake Abiotic stress Increased growth on potassium-free medium 185 G912 OE Freezing Abiotic stress Freezing tolerant Drought Abiotic stress Increased survival in drought Morphology: other Dev and morph conditions Sugar sensing Sugar sensing Dark green color Reduced cotyledon expansion in glucose 187 G913 OE Freezing Abiotic stress Increased tolerance to freezing Flowering time Flowering time Late flowering Drought Abiotic stress Increased tolerance to drought 189 G922 OE Osmotic Abiotic stress Better germination on high sucrose Sodium chloride Abiotic stress Better germination, increased root growth on high salt 191 G926 KO Hormone sensitivity Hormone sensitivity Reduced sensitivity to ABA Osmotic Abiotic stress Increased tolerance to osmotic stress (salt and sucrose) 193 G961 KO Seed oil content Seed biochemistry Increased seed oil content 195 G971 OE Flowering time Flowering time Late flowering 197 G974 OE Seed oil content Seed biochemistry Altered seed oil content 199 G975 OE Leaf fatty acids Leaf biochemistry Increased wax in leaves 201 G979 KO Seed Dev and morph Altered seed development, ripening, and germination 203 G987 KO Leaf fatty acids Leaf biochemistry Reduction in 16:3 fatty acids Leaf prenyl lipids Leaf biochemistry Altered chlorophyll, tocopherol, carotenoid 205 G988 OE Seed protein content Seed biochemistry Increased seed protein content Flower Dev and morph Enlarged floral organs, short pedicels Architecture Dev and morph Reduced lateral branching Stem Dev and morph Thicker stem, altered distribution of vascular bundles 207 G1040 OE Seed Dev and morph Smaller and more rounded seeds 209 G1047 OE Fusarium Disease Increased tolerance to Fusarium 211 G1051 OE Flowering time Flowering time Late flowering 213 G1052 OE Flowering time Flowering time Late flowering 215 G1062 KO Seed Dev and morph Altered seed shape 217 G1063 OE Leaf Dev and morph Altered leaf shape, dark green color Inflorescence Dev and morph Altered inflorescence development Flower Dev and morph Altered flower development, ectopic carpel tissue 219 G1064 OE Botrytis Disease Increased sensitivity to Botrytis 221 G1069 OE Hormone sensitivity Hormone sensitivity Reduced ABA sensitivity Osmotic Abiotic stress Better germination under osmotic stress 223 G1073 OE Size Dev and morph Substantially increased plant size Seed Dev and morph Increased seed yield Drought Abiotic stress Increased tolerance to drought 225 G1075 OE Flower Dev and morph Reduced or absent petals, sepals and stamens 227 G1084 OE Botrytis Disease Increased susceptibility to Botrytis 229 G1089 KO Osmotic Abiotic stress Better germination under osmotic stress 231 G1134 OE Hormone sensitivity Hormone sensitivity Altered response to ethylene: longer hypocotyls and lack of apical hook 233 G1140 OE Flower Dev and morph Altered flower development 235 G1143 OE Seed oil content Seed biochemistry Altered seed oil content 237 G1146 OE Leaf Dev and morph Altered leaf development 239 G1196 KO Botrytis Disease Increased susceptibility to Botrytis 241 G1198 OE Seed oil content Seed biochemistry Increased seed oil content 243 G1225 OE Flowering time Flowering time Early flowering Sugar sensing Sugar sensing Better germination on sucrose and glucose media 245 G1226 OE Seed oil content Seed biochemistry Increased seed oil content 247 G1229 OE Seed oil content Seed biochemistry Decreased seed oil content 249 G1255 OE Botrytis Disease Increased susceptibility to Botrytis Seed Dev and morph Increased seed size Morphology: other Dev and morph Reduced apical dominance 251 G1266 OE Erysiphe Disease Increased tolerance to Erysiphe 253 G1275 OE Architecture Dev and morph Reduced apical dominance 255 G1305 OE Heat Abiotic stress Reduced chlorosis in heat 257 G1322 OE Chilling Abiotic stress Increased seedling vigor in cold Size Dev and morph Reduced size Leaf glucosinolates Leaf biochemistry Increase in M39480 Light response Dev and morph Photomorphogenesis in the dark 259 G1323 OE Seed oil content Seed biochemistry Decreased seed oil Seed protein content Seed biochemistry Increased seed protein 261 G1330 OE Hormone sensitivity Hormone sensitivity Ethylene insensitive when germinated in the dark on ACC 263 G1331 OE Light response Dev and morph Constitutive photomorphogenesis 265 G1332 OE Trichome Dev and morph Reduced trichome density 267 G1363 OE Fusarium Disease Increased tolerance to Fusarium 269 G1411 OE Architecture Dev and morph Loss of apical dominance 271 G1417 KO Seed oil composition Seed biochemistry Increase in 18:2, decrease in 18:3 fatty acids 273 G1419 OE Seed protein content Seed biochemistry Increased seed protein 275 G1449 OE Flower Dev and morph Altered flower structure 277 G1451 OE Morphology: other Dev and morph Increased plant size OE Leaf Dev and morph Large leaf size KO Seed oil content Seed biochemistry Altered seed oil content 279 G1452 OE Trichome Dev and morph Reduced trichome density Leaf Dev and morph Altered leaf shape, dark green color Hormone sensitivity Hormone sensitivity Reduced sensitivity to ABA Osmotic Abiotic stress Better germination on sucrose and salt Flowering time Flowering time Late flowering 281 G1463 OE Senescence Dev and morph Premature senescence 283 G1471 OE Seed oil content Seed biochemistry Increased seed oil content 285 G1478 OE Seed protein content Seed biochemistry Decreased seed protein content Flowering time Flowering time Late flowering Seed oil content Seed biochemistry Increased seed oil content 287 G1482 KO Biochemistry: other Biochem: misc Increased anthocyanins OE Root Dev and morph Increased root growth 289 G1488 OE Seed protein content Seed biochemistry Altered seed protein content Light response Dev and morph Constitutive photomorphogenesis Architecture Dev and morph Reduced apical dominance, shorter stems 291 G1494 OE Flowering time Flowering time Early flowering Light response Dev and morph Long hypocotyls, altered leaf shape Leaf Dev and morph Pale green leaves, altered leaf shape 293 G1496 OE Seed oil content Seed biochemistry Altered seed oil content 295 G1499 OE Morphology: other Dev and morph Dark green color Architecture Dev and morph Altered plant architecture Flower Dev and morph Altered floral organ identity and development 297 G1519 KO Embryo lethal Dev and morph Embryo lethal phenotype: potential herbicide target 299 G1526 KO Seed oil content Seed biochemistry Increased seed oil content 301 G1540 OE Morphology: other Dev and morph Reduced cell differentiation in meristem 303 G1543 OE Architecture Dev and morph Altered architecture, compact plant Morphology: other Dev and morph Dark green color Seed oil content Seed biochemistry Decreased seed oil Leaf prenyl lipids Leaf biochemistry Increase in chlorophyll a and b 305 G1634 OE Seed oil content Seed biochemistry Increased seed oil content Seed protein content Decreased seed protein content 307 G1637 OE Seed protein content Seed biochemistry Altered seed protein content 309 G1640 OE Seed oil content Seed biochemistry Increased seed oil 311 G1645 OE Inflorescence Dev and morph Altered inflorescence structure 313 G1646 OE Seed oil content Seed biochemistry Increased seed oil content 315 G1652 OE Seed protein content Seed biochemistry Increased seed protein content 317 G1672 OE Seed oil content Seed biochemistry Altered seed oil content 319 G1677 OE Seed protein content Seed biochemistry Altered seed protein content Seed oil content Seed biochemistry Altered seed oil content 321 G1749 OE Morphology: other Dev and morph Formation of necrotic lesions 323 G1750 OE Seed oil content Seed biochemistry Increased seed oil content 325 G1756 OE Botrytis Disease Increased susceptibility to Botrytis 327 G1765 OE Seed oil content Seed biochemistry Increased seed oil content 329 G1777 OE Seed oil content Seed biochemistry Increased seed oil content Seed protein content Seed biochemistry Decreased seed protein content 331 G1792 OE Leaf Dev and morph Dark green, shiny leaves Erysiphe Disease Increased resistance to Erysiphe Botrytis Disease Increased resistance to Botrytis Fusarium Disease Increased resistance to Fusarium Nutrient uptake Abiotic stress Increased tolerance to nitrogen- limited medium 333 G1793 OE Seed oil content Seed biochemistry Increased seed oil content 335 G1794 OE Architecture Dev and morph Altered architecture, bushier plant Architecture Dev and morph Reduced apical dominance Light response Dev and morph Constitutive photomorphogenesis Osmotic Abiotic stress Increased sensitivity to high PEG Nutrient uptake Abiotic stress Reduced root growth 337 G1804 OE Flowering time Flowering time Late flowering Sugar sensing Sugar sensing Altered sugar sensing: more sensitive to glucose in germination assays 339 G1818 OE Seed protein content Seed biochemistry Increased protein content 341 G1820 OE Flowering time Flowering time Early flowering Hormone sensitivity Hormone sensitivity Reduced ABA sensitivity Seed protein content Seed biochemistry Increased seed protein content Osmotic Abiotic stress Better germination in high NaCl Drought Abiotic stress Increased tolerance to drought 343 G1836 OE Sodium chloride Abiotic stress Better germination in high salt Drought Abiotic stress Increased tolerance to drought 345 G1838 OE Seed oil content Seed biochemistry Increased seed oil content 347 G1841 OE Heat Abiotic stress Better germination under heat stress Flowering time Flowering time Early flowering 349 G1842 OE Flowering time Flowering time Early flowering 351 G1843 OE Flowering time Flowering time Early flowering 353 G1852 OE Osmotic Abiotic stress Better root growth under osmotic stress 355 G1863 OE Leaf Dev and morph Altered leaf shape and coloration 357 G1880 KO Botrytis Disease Increased resistance to Botrytis 359 G1895 OE Flowering time Flowering time Late flowering 361 G1902 OE Seed oil content Seed biochemistry Increased seed oil content 363 G1903 OE Seed protein content Seed biochemistry Decreased seed protein content 365 G1919 OE Botrytis Disease Increased tolerance to Botrytis 367 G1927 OE Sclerotinia Disease Increased tolerance to Sclerotinia 369 G1930 OE Osmotic Abiotic stress Better germination under osmotic stress 371 G1936 KO Sclerotinia Disease Increased susceptibility to Sclerotinia Botrytis Disease Increased susceptibility to Botrytis 373 G1944 OE Senescence Dev and morph Early senescence 375 G1946 OE Seed oil content Seed biochemistry Increased seed oil content Seed protein content Seed biochemistry Decreased seed protein content Flowering time Flowering time Early flowering Nutrient uptake Abiotic stress Increased root growth on phosphate- free media 377 G1947 KO Fertility Dev and morph Reduced fertility 379 G1948 OE Seed oil content Seed biochemistry Increased seed oil content 381 G1950 OE Botrytis Disease Increased tolerance to Botrytis 383 G1958 KO Morphology: other Dev and morph Reduced size and root mass Seed oil content Seed biochemistry Increased seed oil content Seed protein content Seed biochemistry Increased seed protein content. 385 G2007 OE Flowering time Flowering time Late flowering 387 G2010 OE Flowering time Flowering time Early flowering 389 G2053 OE Osmotic Abiotic stress Increased root growth under osmotic stress 391 G2059 OE Seed oil content Seed biochemistry Altered seed oil content Seed protein content Seed biochemistry Altered seed protein content 393 G2085 OE Seed Dev and morph Increased seed size and altered seed color 395 G2105 OE Seed Dev and morph Large, pale seeds 397 G2110 OE Sodium chloride Abiotic stress Increased tolerance to high salt 399 G2114 OE Seed Dev and morph Increased seed size 401 G2117 OE Seed protein content Seed biochemistry Increased seed protein content 403 G2123 OE Seed oil content Seed biochemistry Increased seed oil content 405 G2130 OE Heat Abiotic stress Better germination in heat 407 G2133 OE Glyphosate Herbicide sensitivity Increased tolerance to glyphosate Flowering time Flowering time Late flowering 409 G2138 OE Seed oil content Seed biochemistry Increased seed oil content 411 G2140 OE Hormone sensitivity Hormone sensitivity Decreased sensitivity to ABA Osmotic Abiotic stress Better germination on high NaCl and sucrose 413 G2143 OE Inflorescence Dev and morph Altered inflorescence development Leaf Dev and morph Altered leaf shape, dark green color Flower Dev and morph Altered flower development, ectopic carpel tissue 415 G2144 OE Flowering time Flowering time Early flowering Leaf Dev and morph Pale green leaves, altered leaf shape Light response Dev and morph Long hypocotyls, altered leaf shape 417 G2153 OE Osmotic Abiotic stress Better germination under osmotic stress 419 G2155 OE Flowering time Flowering time Late flowering 421 G2192 OE Seed oil composition Seed biochemistry Altered seed fatty acid composition 423 G2295 OE Flowering time Flowering time Early flowering 425 G2340 OE Seed glucosinolates Seed biochemistry Altered glucosinolate profile 427 G2343 OE Seed oil content Seed biochemistry Increased seed oil content 429 G2346 OE Morphology: other Dev and morph Enlarged seedlings 431 G2347 OE Flowering time Flowering time Early flowering 433 G2379 OE Osmotic Abiotic stress Increased seedling vigor on high sucrose media 435 G2430 OE Heat Abiotic stress Increased tolerance to heat Size Dev and morph Increased leaf size, faster development 437 G2505 OE Drought Abiotic stress Increased tolerance to drought 439 G2509 OE Seed oil content Seed biochemistry Decreased seed oil content Seed protein content Seed biochemistry Increased seed protein content Seed prenyl lipids Seed biochemistry Increase in alpha-tocopherol Architecture Dev and morph Reduced apical dominance Flowering time Flowering time Early flowering 441 G2517 OE Glyphosate Herbicide sensitivity Increased tolerance to glyphosate 443 G2520 OE Seed prenyl lipids Seed biochemistry Altered tocopherol composition 445 G2555 OE Light response Dev and morph Constitutive photomorphogenesis Botrytis Disease Increased susceptibility to Botrytis 447 G2557 OE Leaf Dev and morph Altered leaf shape, dark green color Flower Dev and morph Altered flower development, ectopic carpel tissue 449 G2583 OE Leaf Dev and morph Glossy, shiny leaves 451 G2701 OE Osmotic Abiotic stress Better germination on high NaCl and sucrose 453 G2719 OE Osmotic Abiotic stress Increased seedling vigor on high sucrose 455 G2789 OE Osmotic Abiotic stress Better germination on high sucrose Hormone sensitivity Hormone sensitivity Reduced ABA sensitivity 457 G2830 KO Seed oil content Seed biochemistry Increased seed oil content 1951 G12 KO Hormone sensitivity Hormone sensitivity Increased sensitivity to ACC OE Morphology: other Dev and morph Leaf and hypocotyl necrosis 1953 G30 OE Leaf Dev and morph Glossy green leaves Light response Dev and morph Shade avoidance 1975 G231 OE Leaf fatty acids Leaf biochemistry Increased leaf unsaturated fatty acids Seed oil content Seed biochemistry Increased seed oil content Seed protein content Seed biochemistry Decreased seed protein content 1979 G247 OE Trichome Dev and morph Altered trichome distribution, reduced trichome density 1991 G370 KO Size Dev and morph Reduced size, shiny leaves OE Trichome Dev and morph Ectropic trichome formation 2009 G485 OE Flowering time Flowering time Early flowering KO Flowering time Flowering time Late flowering 2061 G839 OE Nutrient uptake Abiotic stress Increased tolerance to nitrogen- limited medium 2099 G1357 OE Leaf Dev and morph Altered leaf shape, dark green leaves Chilling Abiotic stress Increased tolerance to cold Hormone sensitivity Hormone sensitivity Insensitive to ABA Flowering time Flowering time Late flowering 2126 G1646 OE Seed oil content Seed oil content Increased seed oil content 2142 G1816 OE Sugar sensing Sugar sensing Increased tolerance to glucose Nutrient uptake Abiotic stress Altered C/N sensing; less anthocyanin on nitrogen-limited medium Osmotic Abiotic stress Increased tolerance to osmotic stress Root Dev and morph Increased root hairs Trichome Dev and morph Glabrous leaves Nutrient uptake Abiotic stress Increased tolerance to nitrogen- limited medium 2147 G1888 OE Size Dev and morph Reduced size, dark green leaves 2153 G1945 OE Flowering time Flowering time Late flowering Leaf Dev and morph Altered leaf shape 2195 G2826 OE Flower Dev and morph Aerial rosettes Trichome Dev and morph Ectropic trichome formation 2197 G2838 OE Trichome Dev and morph Increased trichome density Flowering time Flosering time Late flowering Flower Dev and morph Flower: multiple alterations Flower Dev and morph Aerial rosettes Leaves Dev and morph Dark green leaves Size Dev and morph Increased seedling size 2199 G2839 OE Osmotic stress Dev and morph Better germination on high sucrose Inflorescence Dev and morph Downward pedicels Size Abiotic stress Reduced size

Table 5 shows the polypeptides identified by SEQ ID NO; Mendel Gene ID (GID) No.; the transcription factor family to which the polypeptide belongs, and conserved domains of the polypeptide. The first column shows the polypeptide SEQ ID NO; the third column shows the transcription factor family to which the polynucleotide belongs; and the fourth column shows the amino acid residue positions of the conserved domain in amino acid (AA) co-ordinates.

TABLE 5 Gene families and conserved domains Polypeptide GID Conserved Domains in SEQ ID NO: No. Family Amino Acid Coordinates 2 G8 AP2 151-217, 243-296 4 G19 AP2  76-145 6 G22 AP2  89-157 8 G24 AP2  25-93 10 G28 AP2 145-213 12 G47 AP2 11-80 14 G156 MADS  2-57 16 G157 MADS  2-57 18 G162 MADS  2-57 20 G175 WRKY 178-234, 372-428 22 G180 WRKY 118-174 24 G183 WRKY 307-363 26 G188 WRKY 175-222 28 G189 WRKY 240-297 30 G192 WRKY 128-185 32 G196 WRKY 223-283 34 G211 MYB-R1 R2R3  24-137 36 G214 MYB-related 22-71 38 G226 MYB-related 28-78 40 G241 MYB-R1 R2R3  14-114 42 G248 MYB-R1 R2R3 264-332 44 G254 MYB-related  62-106 46 G256 MYB-R1 R2R3  13-115 48 G278 AKR  2-593 50 G291 MISC 132-160 52 G303 HLH/MYC 92-161 54 G312 SCR 320-336 56 G325 Z-CO-like 5-28, 48-71 58 G343 GATA/Zn 178-214 60 G353 Z-C2H2 41-61, 84-104 62 G354 Z-C2H2 42-62, 88-109 64 G361 Z-C2H2 43-63 66 G362 Z-C2H2 62-82 68 G371 RING/C3HC4 21-74 70 G390 HB 18-81 72 G391 HB 25-85 74 G409 HB 64-124 76 G427 HB 307-370 78 G438 HB 22-85 80 G450 IAA 6-14, 78-89, 112-128, 180-213 82 G464 IAA 20-28, 71-82, 126-142, 187-224 84 G470 ARF 61-393 86 G477 SBP 108-233 88 G481 CAAT 20-109 90 G482 CAAT 25-116 92 G484 CAAT 11-104 94 G489 CAAT 57-156 96 G490 CAAT 48-143 98 G504 NAC 19-174 100 G509 NAC  13-169 102 G519 NAC  11-104 104 G545 Z-C2H2 82-102, 136-154 106 G546 RING/C3H2C3 114-155 108 G561 bZIP 248-308 110 G562 bZIP 253-315 112 G567 bZIP 210-270 114 G568 bZIP 215-265 116 G584 HLH/MYC 401-494 118 G585 HLH/MYC 436-501 120 G590 HLH/MYC 202-254 122 G594 HLH/MYC 140-204 124 G597 AT-hook 97-104, 137-144 126 G598 DBP 205-263 128 G634 TH 62-147, 189-245 130 G635 TH 239-323 132 G636 TH 55-145, 405-498 134 G638 TH 119-206 136 G652 Z-CLDSH 28-49, 137-151, 182-196 138 G663 MYB-R1 R2R3  9-111 140 G664 MYB-R1 R2R3  13-116 142 G674 MYB-R1 R2R3  20-120 144 G676 MYB-R1 R2R3  17-119 146 G680 MYB-related 24-70 148 G682 MYB-related 27-63 150 G715 CAAT  60-132 152 G720 GARP 301-349 154 G736 Z-Dof  54-111 156 G748 Z-Dof 112-140 158 G779 HLH/MYC 126-182 160 G789 HLH/MYC 253-313 162 G801 PCF 32-93 164 G849 BPF-1 324-413, 504-583 166 G859 MADS  3-56 168 G864 AP2 119-186 170 G867 AP2  59-124 172 G869 AP2 109-177 174 G877 WRKY 272-328, 487-603 176 G881 WRKY 176-233 178 G892 RING/C3H2C3 177-270 180 G896 Z-LSDlike 18-39 182 G910 Z-CO-like 14-37, 77-103 184 G911 RING/C3H2C3  86-129 186 G912 AP2  51-118 188 G913 AP2  62-128 190 G922 SCR 225-242 192 G926 CAAT 131-225 194 G961 NAC  15-140 196 G971 AP2 120-186 198 G974 AP2  81-140 200 G975 AP2  4-71 202 G979 AP2 63-139, 165-233 204 G987 SCR 428-432, 704-708 206 G988 SCR 178-195 208 G1040 GARP 109-158 210 G1047 bZIP 129-180 212 G1051 bZIP 189-250 214 G1052 bZIP 201-261 216 G1062 HLH/MYC 308-359 218 G1063 HLH/MYC 131-182 220 G1064 PCF 116-179 222 G1069 AT-hook 67-74 224 G1073 AT-hook 33-42, 78-175 226 G1075 AT-hook 78-85 228 G1084 BZIPT2 1-53, 490-619 230 G1089 BZIPT2 425-500 232 G1134 HLH/MYC 198-247 234 G1140 MADS  2-57 236 G1143 HLH/MYC 33-82 238 G1146 PAZ 886-896 240 G1196 AKR 179-254 242 G1198 bZIP 173-223 244 G1225 HLH/MYC  78-147 246 G1226 HLH/MYC 115-174 248 G1229 HLH/MYC 102-160 250 G1255 Z-CO-like 18-56 252 G1266 AP2  79-147 254 G1275 WRKY 113-169 256 G1305 MYB-R1 R2R3  15-118 258 G1322 MYB-R1 R2R3  26-130 260 G1323 MYB-R1 R2R3  15-116 262 G1330 MYB-R1 R2R3  28-134 264 G1331 MYB-R1 R2R3  8-109 266 G1332 MYB-R1 R2R3  13-116 268 G1363 CAAT 174-226 270 G1411 AP2  87-154 272 G1417 WRKY 239-296 274 G1419 AP2  69-137 276 G1449 IAA 48-53, 74-107, 122-152 278 G1451 ARF  22-357 280 G1452 NAC  30-177 282 G1463 NAC  9-156 284 G1471 Z-C2H2 49-70 286 G1478 Z-CO-like 32-76 288 G1482 Z-CO-like  5-63 290 G1488 GATA/Zn 221-246 292 G1494 HLH/MYC 261-311 294 G1496 HLH/MYC 184-248 296 G1499 HLH/MYC 118-181 298 G1519 RING/C3HC4 327-364 300 G1526 SWI/SNF 493-620, 864-1006 302 G1540 HB 35-98 304 G1543 HB 135-195 306 G1634 MYB-related 129-180 308 G1637 MYB-related 109-173 310 G1640 MYB-R1 R2R3  14-115 312 G1645 MYB-R1 R2R3  90-210 314 G1646 CAAT  72-162 316 G1652 HLH/MYC 143-215 318 G1672 NAC  41-194 320 G1677 NAC  17-181 322 G1749 AP2  84-155 324 G1750 AP2 107-173 326 G1756 WRKY 141-197 328 G1765 NAC  20-140 330 G1777 RING/C3HC4 124-247 332 G1792 AP2 17-85 334 G1793 AP2 179-255, 281-349 336 G1794 AP2 182-249 338 G1804 bZIP 357-407 340 G1818 CAAT  36-113 342 G1820 CAAT  70-133 344 G1836 CAAT  30-164 346 G1838 AP2 229-305, 330-400 348 G1841 AP2  83-150 350 G1842 MADS  2-57 352 G1843 MADS  2-57 354 G1852 AKR  1-600 356 G1863 GRF-like  77-186 358 G1880 Z-C2H2 69-89, 111-139 360 G1895 Z-Dof  55-110 362 G1902 Z-Dof 31-59 364 G1903 Z-Dof 134-180 366 G1919 RING/C3HC4 214-287 368 G1927 NAC  17-188 370 G1930 AP2  59-124 372 G1936 PCF  64-129 374 G1944 AT-hook  87-100 376 G1946 HS  32-130 378 G1947 HS  37-120 380 G1948 AKR 75-126, 120-148, 152-181, 186-215, 261-311, 312-363 382 G1950 AKR 65-228 384 G1958 GARP 230-278 386 G2007 MYB-R1 R2R3  14-116 388 G2010 SBP  53-127 390 G2053 NAC  10-149 392 G2059 AP2 184-254 394 G2085 RING/C3HC4 214-241 396 G2105 TH 100-153 398 G2110 WRKY 239-298 400 G2114 AP2 221-297, 323-393 402 G2117 bZIP  46-106 404 G2123 GF14  99-109 406 G2130 AP2  93-160 408 G2133 AP2 11-83 410 G2138 AP2  76-148 412 G2140 HLH/MYC 167-242 414 G2143 HLH/MYC 128-179 416 G2144 HLH/MYC 203-283 418 G2153 AT-hook 75-94, 162-206 420 G2155 AT-hook 18-38 422 G2192 bZIP-NIN 600-700 424 G2295 MADS  2-57 426 G2340 MYB-R1 R2R3  14-120 428 G2343 MYB-R1 R2R3  14-116 430 G2346 SBP  59-135 432 G2347 SBP  60-136 434 G2379 TH 19-110, 173-232 436 G2430 GARP 425-478 438 G2505 NAC  10-159 440 G2509 AP2  89-156 442 G2517 WRKY 118-174 444 G2520 HLH/MYC 135-206 446 G2555 HLH/MYC 175-245 448 G2557 HLH/MYC 278-328 450 G2583 AP2  4-71 452 G2701 MYB-related 33-81, 129-183 454 G2719 MYB-R1 R2R3  56-154 456 G2789 AT-hook 53-73, 121-165 458 G2830 Z-C2H2 245-266

Examples of some of the utilities that may be desirable in plants, and that may be provided by transforming the plants with the presently disclosed sequences, are listed in Table 6. Many of the transcription factors listed in Table 6 may be operably linked with a specific promoter that causes the transcription factor to be expressed in response to environmental, tissue-specific or temporal signals. For example, G362 induces ectopic trichomes on flowers but also produces small plants. The former may be desirable to produce insect or herbivore resistance, or increased cotton yield, but the latter may be undesirable in that it may reduce biomass. However, by operably linking G362 with a flower-specific promoter, one may achieve the desirable benefits of the gene without affecting overall biomass to a significant degree. For examples of flower specific promoters, see Kaiser et al. (supra). For examples of other tissue-specific, temporal-specific or inducible promoters, see the above discussion under the heading “Vectors, Promoters, and Expression Systems”.

TABLE 6 Genes, traits and utilities that affect plant characteristics Transcription factor genes Trait Category Phenotype(s) that impact traits Utility Abiotic stress Effect of chilling on plants Increased tolerance: G256; G664; G1322 Improved germination, growth rate, earlier planting, yield Germination in cold Increased tolerance: G256; G664 Earlier planting; improved survival, yield Freezing tolerance G720 (G720 KO is more Earlier planting; susceptible); G912; G913 improved quality, survival, yield Drought Increased tolerance: G912; G913; G1820; G1836; Improved survival, G2505 vigor, appearance, yield Heat Increased tolerance: G464; G682; G864; G1305; Improved germination, G1841; G2130; G2430 growth rate, later planting, yield Osmotic stress Increased sensitivity: G1794 Abiotic stress response manipulation Increased tolerance: G47; G175; G188; G303; G325; Improved germination G353; G489; G922; G926; rate, seedling vigor, G1069; G1089; G1452; G1816; survival, yield G1820; G1852; G1930; G2053; G2140; G2153; G2379; G2701; G2719; G2789; G2839 Salt tolerance More susceptible: G545 Manipulation of response to high salt conditions Increased tolerance: G22; G196; G226; G312; G482; Improved germination G801; G867; G922; G1836; rate, survival, yield; G2110 extended growth range Nitrogen stress Sensitivity to N limitation: G1794 Manipulation of response to low nutrient conditions Tolerance to N limitation: G225; G226; G839; G1792; Improved yield and G1816 nutrient stress tolerance, decreased fertilizer usage Phosphate stress Tolerance to P limitation: G545; G561; G911; G1946 Improved yield and nutrient stress tolerance, decreased fertilizer usage Oxidative stress G477 Improved yield, quality, ultraviolet and chemical stress tolerance Herbicide Glyphosate G343; G2133; G2517 Generation of glyphosate-resistant plants to improve weed control Hormone Abscisic acid (ABA) sensitivity sensitivity Reduced sensitivity to ABA: G546; G926; G1069; G1357; Modification of seed G1452; G1820; G2140; G2789 development, improved seed dormancy, cold and dehydration tolerance Sensitivity to ethylene Altered response: G1134 Manipulation of fruit Insensitive to ethylene: G1330 ripening Disease Botrytis Increased susceptibility: G248; G371; G1064; G1084; Manipulation of G1196; G1255; G1756; G1936; response to disease G2555 organism Increased resistance or G28; G1792; G1880; G1919; Improved yield, tolerance: G1950 appearance, survival, extended range Fusarium Increased susceptibility: G188; G545; G896 Manipulation of response to disease organism Increased resistance or G1047; G1792 Improved yield, tolerance: appearance, survival, extended range Erysiphe Increased susceptibility: G545; G881 Manipulation of response to disease organism Increased resistance or G19; G28; G409; G1266; Improved yield, tolerance: G1363; G1792 appearance, survival, extended range Pseudomonas Increased susceptibility: G545 Manipulation of response to disease organism Sclerotinia Increased susceptibility: G278; G477; G594; G1936 Manipulation of response to disease organism Increased resistance or G28; G1927 Improved yield, tolerance: appearance, survival, extended range Growth regulator Altered sugar sensing Decreased tolerance to sugars: G241; G254; G567; G680; Alteration of energy G912; G1804 balance, photosynthetic Increased tolerance to sugars: G481; G867; G1225; G1816 rate, carbohydrate accumulation, biomass production, source-sink relationships, senescence; alteration of storage compound accumulation in seeds Altered C/N sensing G1816 Flowering time Early flowering G157; G180; G183; G485 (OE); Faster generation time; G490; G590; G789; G1225; synchrony of flowering; G1494; G1820; G1841; G1842; additional harvests G1843; G1946; G2010; G2144; within a growing season, G2295; G2347; G2509 shortening of breeding programs Late flowering G8; G47; G157; G192; G214; Increased yield or G231; G361; G362; G485 (KO); biomass, alleviate risk of G562; G736; G748; G859; transgenic pollen escape, G910; G913; G971; G1051; synchrony of flowering G1052; G1357; G1452; G1478; G1804; G1895; G1945; G2007; G2133; G2155; G2838 General Altered flower structure development and Stamen: G988; G1075; G1140; G1499; Ornamental morphology G2557 modification of plant Sepal: G1075; G1140; G2557 architecture, improved Petal: G638; G1075; G1140; G1449; or reduced fertility to G1499; G2557 mitigate escape of Pedicel: G353; G354; G988 transgenic pollen, Carpel: G1063; G1140; G2143; G2143; improved fruit size, G2557 shape, number or yield Multiple alterations: G638; G988; G1063; G1140; G1449; G1499; G2143; G2557 G988; G1449; G2838 Enlarged floral organs: G353; G354 Siliques: G470; G779; G988; G1075; G1140; G1499; G1947; G2143; G2557 Reduced fertility: G638; G779; G1140; G1499 Aerial rosettes G1995; G2826; G2838 Inflorescence architectural change Altered branching pattern: G47; G1063; G1645; G2143 Ornamental Short internodes/bushy G47 modification of flower inflorescences: architecture; timing of Internode elongation: G1063 flowering; altered plant Lack of inflorescence: G1499; G2143 habit for yield or harvestability benefit; reduction in pollen production of genetically modified plants; manipulation of seasonality and annual or perennial habit; manipulation of determinate vs. indeterminate growth Altered shoot meristem development Stem bifurcations: G390; G391 Ornamental modification of plant architecture, manipulation of growth and development, increase in leaf numbers, modulation of branching patterns to provide improved yield or biomass Altered branching pattern G427; G568; G988; G1543; Ornamental G1794 modification of plant architecture, improved lodging resistance Apical dominance Reduced apical dominance: G47; G211; G1255; G1275; Ornamental G1411; G1488; G1794; G2509 modification of plant architecture Altered trichome density; development, or structure Reduced or no trichomes: G225; G226; G247; G585; Ornamental G676; G682; G1332; G1452; modification of plant G1816 architecture, increased Ectopic trichomes/altered G247; G362; G370; G676; plant product (e.g., trichome development/cell G2826 diterpenes, cotton) fate: productivity, insect and Increase in trichome number, G362; G634; G838; G2838 herbivore resistance size or density: Stem morphology and altered G47; G438; G748; G988; Modulation of lignin vascular tissue structure G1488 content; improvement of wood, palatability of fruits and vegetables Root development Increased root growth and G1482 Improved yield, stress proliferation: tolerance; anchorage Increased root hairs: G225; G226; G1816 Altered seed development, G979 ripening and germination Cell differentiation and cell G1540 Increase in carpel or proliferation fruit development; improve regeneration of shoots from callus in transformation or micro- propagation systems Rapid development G2430 Promote faster development and reproduction in plants Senescence Premature senescence: G636; G1463; G1944 Improvement in response to disease, fruit ripening Lethality when overexpressed G877; G1519 Herbicide target; ablation of specific tissues or organs such as stamen to prevent pollen escape Necrosis G12, G24 Disease resistance Plant size Increased plant size G1073; G1451 Improved yield, biomass, appearance Larger seedlings G2346; G2838 Increased survival and vigor of seedlings, yield Dwarfed or more compact G24; G343; G353; G354; G362; Dwarfism, lodging plants G370; G1008; G1277; G1543; resistance, manipulation G1794; G1958 of gibberellin responses Leaf morphology Dark green leaves G674; G912; G1063; G1357; Increased G1452; G1482; G1499; G1792; photosynthesis, biomass, G1863; G1888; G2143; G2557; appearance, yield G2838 Change in leaf shape G211; G353; G674; G736; Ornamental applications G1063; G1146; G1357; G1452; G1494; G1543; G1863; G2143; G2144 Altered leaf size: Increased leaf size, number or G189; G214; G1451; G2430 Increased yield, mass: ornamental applications Light green leaves G1494; G2144 Ornamental applications Variegation G635 Ornamental applications Glossy leaves G30; G1792; G2583 Ornamental applications, manipulation of wax composition, amount, or distribution Seed morphology Altered seed coloration G156; G2105; G2085 Appearance Seed size and shape Increased seed size: G450; G584; G1255; G2085; Yield, appearance G2105; G2114 Decreased seed size: G1040 Appearance Altered seed shape: G1040; G1062 Appearance Leaf biochemistry Increased leaf wax G975; G1792; G2583 Insect, pathogen resistance Leaf prenyl lipids Reduced chlorophyll: G987 Increase in tocopherols G652; G987; G2509 Increased lutein content G748 Increase in chlorophyll or G214; G1543 carotenoids: Leaf insoluble sugars Increase in leaf xylose G211 Increased leaf anthocyanins G663; G1482; G1888 Leaf fatty acids Reduction in leaf fatty acids: G987 Increase in leaf fatty acids: G214 Seed Seed oil content biochemistry Increased oil content: G162; G291; G427; G509; Improved oil yield G519; G561; G590; G598; G629; G715; G849; G961; G1198; G1226; G1471; G1478; G1526; G1640; G1646; G1750; Reduced caloric content G1765; G1777; G1793; G1838; G1902; G1946; G1948; G1958, G2123; G2138; G2343; G2830 Decreased oil content: G180; G192; G241; G504; G1143; G1229; G1323; G1543; G2509 Altered oil content: G567; G892; G974; G1451; G1496; G1646; G1672; G1677 Altered fatty acid content: G869; G1417; G2192 Seed protein content Increased protein content: G162; G226; G241; G509; Improved protein yield, G988; G1323; G1419; G1652; nutritional value G1818; G1820; G1958; G2117; Reduced caloric content G2509 Decreased protein content: G427; G1478; G1777; G1903; G1946 Altered protein content: G162; G567; G597; G849; G892; G1634; G1637; G1677 Altered seed prenyl lipid G652; G2509; G2520 Improved antioxidant content or composition and vitamin E content Seed glucosinolate Altered profile: G484; G2340 Increased seed anthocyanins G362; G663 Root Increased root anthocyanins G663 Biochemistry Light Altered cotyledon, hypocotyl, G183; G354; G1322; G1331; Potential for increased response/shade petiole development; altered G1488; G1494; G1794; G2144; planting densities and avoidance leaf orientation; constitutive G2555 yield enhancement photomorphogenesis; photomorphogenesis in low light Pigment Increased anthocyanin level G362; G663; G1482 Enhanced health benefits, improved ornamental appearance, increased stress resistance, attraction of pollinating and seed- dispersing animals Abbreviations: N = nitrogen P = phosphate ABA = abscisic acid C/N = carbon/nitrogen balance Detailed Description of Genes, Traits and Utilities that Affect Plant Characteristics

The following descriptions of traits and utilities associated with the present transcription factors offer a more comprehensive description than that provided in Table 6.

Abiotic Stress, General Considerations

Plant transcription factors can modulate gene expression, and, in turn, be modulated by the environmental experience of a plant. Significant alterations in a plant's environment invariably result in a change in the plant's transcription factor gene expression pattern. Altered transcription factor expression patterns generally result in phenotypic changes in the plant. Transcription factor gene product(s) in transgenic plants then differ(s) in amounts or proportions from that found in wild-type or non-transformed plants, and those transcription factors likely represent polypeptides that are used to alter the response to the environmental change. By way of example, it is well accepted in the art that analytical methods based on altered expression patterns may be used to screen for phenotypic changes in a plant far more effectively than can be achieved using traditional methods.

Abiotic stress: adult stage chilling. Enhanced chilling tolerance may extend the effective growth range of chilling sensitive crop species by allowing earlier planting or later harvest. Improved chilling tolerance may be conferred by increased expression of glycerol-3-phosphate acetyltransferase in chloroplasts (see, for example, Wolter et al. (1992) et al. EMBO J. 4685-4692, and Murata et al. (1992) Nature 356: 710-713).

Chilling tolerance could also serve as a model for understanding how plants adapt to water deficit. Both chilling and water stress share similar signal transduction pathways and tolerance/adaptation mechanisms. For example, acclimation to chilling temperatures can be induced by water stress or treatment with abscisic acid. Genes induced by low temperature include dehydrins (or LEA proteins). Dehydrins are also induced by salinity, abscisic acid, water stress, and during the late stages of embryogenesis.

Another large impact of chilling occurs during post-harvest storage. For example, some fruits and vegetables do not store well at low temperatures (for example, bananas, avocados, melons, and tomatoes). The normal ripening process of the tomato is impaired if it is exposed to cool temperatures. Transcription factor genes conferring resistance to chilling temperatures, including G256, G664, and G1322 may thus enhance tolerance during post-harvest storage.

Abiotic stress: cold germination. Several of the presently disclosed transcription factor genes confer better germination and growth in cold conditions. For example, the improved germination in cold conditions seen with G256 and G664 indicates a role in regulation of cold responses by these genes and their equivalogs. These genes might be engineered to manipulate the response to low temperature stress. Genes that would allow germination and seedling vigor in the cold would have highly significant utility in allowing seeds to be planted earlier in the season with a high rate of survival. Transcription factor genes that confer better survival in cooler climates allow a grower to move up planting time in the spring and extend the growing season further into autumn for higher crop yields. Germination of seeds and survival at temperatures significantly below that of the mean temperature required for germination of seeds and survival of non-transformed plants would increase the potential range of a crop plant into regions in which it would otherwise fail to thrive.

Abiotic stress: freezing tolerance and osmotic stress. Presently disclosed transcription factor genes, including G47, G175, G188, G303, G325, G353, G489, G922, G926, G1069, G1089, G1452, G1820, G1852, G1930, G2053, G2140, G2153, G2379, G2701, G2719, G2789, G2839 and their equivalogs, that increase germination rate and/or growth under adverse osmotic conditions, could impact survival and yield of seeds and plants. Osmotic stresses may be regulated by specific molecular control mechanisms that include genes controlling water and ion movements, functional and structural stress-induced proteins, signal perception and transduction, and free radical scavenging, and many others (Wang et al. (2001) Acta Hort. (ISHS) 560: 285-292). Instigators of osmotic stress include freezing, drought and high salinity, each of which are discussed in more detail below.

In many ways, freezing, high salt and drought have similar effects on plants, not the least of which is the induction of common polypeptides that respond to these different stresses. For example, freezing is similar to water deficit in that freezing reduces the amount of water available to a plant. Exposure to freezing temperatures may lead to cellular dehydration as water leaves cells and forms ice crystals in intercellular spaces (Buchanan, supra). As with high salt concentration and freezing, the problems for plants caused by low water availability include mechanical stresses caused by the withdrawal of cellular water. Thus, the incorporation of transcription factors that modify a plant's response to osmotic stress or improve tolerance to (e.g., by G720, G912, G913 or their equivalogs) into, for example, a crop or ornamental plant, may be useful in reducing damage or loss. Specific effects caused by freezing, high salt and drought are addressed below.

Abiotic stress: drought and low humidity tolerance. Exposure to dehydration invokes similar survival strategies in plants as does freezing stress (see, for example, Yelenosky (1989) Plant Physiol 89: 444-451) and drought stress induces freezing tolerance (see, for example, Siminovitch et al. (1982) Plant Physiol 69: 250-255; and Guy et al. (1992) Planta 188: 265-270). In addition to the induction of cold-acclimation proteins, strategies that allow plants to survive in low water conditions may include, for example, reduced surface area, or surface oil or wax production. A number of presently disclosed transcription factor genes, e.g., G912, G913, G1820, G1836 and G2505 increase a plant's tolerance to low water conditions and, along with their functional equivalogs, would provide the benefits of improved survival, increased yield and an extended geographic and temporal planting range.

Abiotic stress: heat stress tolerance. The germination of many crops is also sensitive to high temperatures. Presently disclosed transcription factor genes that provide increased heat tolerance, including G464, G682, G864, G1305, G1841, G2130, G2430 and their equivalogs, would be generally useful in producing plants that germinate and grow in hot conditions, may find particular use for crops that are planted late in the season, or extend the range of a plant by allowing growth in relatively hot climates.

Abiotic stress: salt. The genes in Table 6 that provide tolerance to salt may be used to engineer salt tolerant crops and trees that can flourish in soils with high saline content or under drought conditions. In particular, increased salt tolerance during the germination stage of a plant enhances survival and yield. Presently disclosed transcription factor genes, including G22, G196, G226, G312, G482, G801, G867, G922, G1836, G2110, and their equivalogs that provide increased salt tolerance during germination, the seedling stage, and throughout a plant's life cycle, would find particular value for imparting survival and yield in areas where a particular crop would not normally prosper.

Nutrient uptake and utilization: nitrogen and phosphorus. Presently disclosed transcription factor genes introduced into plants provide a means to improve uptake of essential nutrients, including nitrogenous compounds, phosphates, potassium, and trace minerals. The enhanced performance of, for example, G225, G226, G839, G1792, and other overexpressing lines under low nitrogen, and G545, G561, G911, G1946 under low phosphorous conditions indicate that these genes and their equivalogs can be used to engineer crops that could thrive under conditions of reduced nutrient availability. Phosphorus, in particular, tends to be a limiting nutrient in soils and is generally added as a component in fertilizers. Young plants have a rapid intake of phosphate and sufficient phosphate is important for yield of root crops such as carrot, potato and parsnip.

The effect of these modifications is to increase the seedling germination and range of ornamental and crop plants. The utilities of presently disclosed transcription factor genes conferring tolerance to conditions of low nutrients also include cost savings to the grower by reducing the amounts of fertilizer needed, environmental benefits of reduced fertilizer runoff into watersheds; and improved yield and stress tolerance. In addition, by providing improved nitrogen uptake capability, these genes can be used to alter seed protein amounts and/or composition in such a way that could impact yield as well as the nutritional value and production of various food products.

A number of the transcription factor-overexpressing lines make less anthocyanin on high sucrose plus glutamine indicates that these genes can be used to modify carbon and nitrogen status, and hence assimilate partitioning (assimilate partitioning refers to the manner in which an essential element, such as nitrogen, is distributed among different pools inside a plant, generally in a reduced form, for the purpose of transport to various tissues).

Increased tolerance of plants to oxidative stress. In plants, as in all living things, abiotic and biotic stresses induce the formation of oxygen radicals, including superoxide and peroxide radicals. This has the effect of accelerating senescence, particularly in leaves, with the resulting loss of yield and adverse effect on appearance. Generally, plants that have the highest level of defense mechanisms, such as, for example, polyunsaturated moieties of membrane lipids, are most likely to thrive under conditions that introduce oxidative stress (e.g., high light, ozone, water deficit, particularly in combination). Introduction of the presently disclosed transcription factor genes, including G477 and its equivalogs, that increase the level of oxidative stress defense mechanisms would provide beneficial effects on the yield and appearance of plants. One specific oxidizing agent, ozone, has been shown to cause significant foliar injury, which impacts yield and appearance of crop and ornamental plants. In addition to reduced foliar injury that would be found in ozone resistant plant created by transforming plants with some of the presently disclosed transcription factor genes, the latter have also been shown to have increased chlorophyll fluorescence (Yu-Sen Chang et al. (2001) Bot. Bull. Acad. Sin. 42: 265-272).

Decreased herbicide sensitivity. Presently disclosed transcription factor genes, including G343, G2133, G2517 and their equivalogs, that confer resistance or tolerance to herbicides (e.g., glyphosate) will find use in providing means to increase herbicide applications without detriment to desirable plants. This would allow for the increased use of a particular herbicide in a local environment, with the effect of increased detriment to undesirable species and less harm to transgenic, desirable cultivars.

Knockouts of a number of the presently disclosed transcription factor genes have been shown to be lethal to developing embryos. Thus, these genes are potentially useful as herbicide targets.

Hormone sensitivity. ABA plays regulatory roles in a host of physiological processes in all higher as well as in lower plants (Davies et al. (1991) Abscisic Acid: Physiology and Biochemistry. Bios Scientific Publishers, Oxford, UK; Zeevaart et al. (1988) Ann Rev Plant Physiol. Plant Mol. Biol. 49: 439-473; Shimizu-Sato et al. (2001) Plant Physiol 127: 1405-1413). ABA mediates stress tolerance responses in higher plants, is a key signal compound that regulates stomatal aperture and, in concert with other plant signaling compounds, is implicated in mediating responses to pathogens and wounding or oxidative damage (for example, see Larkindale et al. (2002) Plant Physiol. 128: 682-695). In seeds, ABA promotes seed development, embryo maturation, synthesis of storage products (proteins and lipids), desiccation tolerance, and is involved in maintenance of dormancy (inhibition of germination), and apoptosis (Zeevaart et al. (1988) Ann Rev Plant Physiol. Plant Mol. Biol. 49: 439-473; Davies (1991), supra; Thomas (1993) Plant Cell 5: 1401-1410; and Bethke et al. (1999) Plant Cell 11: 1033-1046). ABA also affects plant architecture, including root growth and morphology and root-to-shoot ratios. ABA action and metabolism is modulated not only by environmental signals but also by endogenous signals generated by metabolic feedback, transport, hormonal cross-talk and developmental stage. Manipulation of ABA levels, and hence by extension the sensitivity to ABA, has been described as a very promising means to improve productivity, performance and architecture in plants Zeevaart (1999) in: Biochemistry and Molecular Biology of Plant Hormones, Hooykaas et al. eds, Elsevier Science pp 189-207; and Cutler et al. (1999) Trends Plant Sci. 4: 472-478).

A number of the presently disclosed transcription factor genes affect plant abscisic acid (ABA) sensitivity, including G546, G926, 1069, G1357, G1452, G1820, G2140, G2789. Thus, by affecting ABA sensitivity, these introduced transcription factor genes and their equivalogs would affect cold, drought, oxidative and other stress sensitivities, plant architecture, and yield.

Several other of the present transcription factor genes have been used to manipulate ethylene signal transduction and response pathways. These genes can thus be used to manipulate the processes influenced by ethylene, such as seed germination or fruit ripening, and to improve seed or fruit quality.

Diseases, pathogens and pests. A number of the presently disclosed transcription factor genes have been shown to or are likely to affect a plants response to various plant diseases, pathogens and pests. The offending organisms include fungal pathogens Fusarium oxysporum, Botrytis cinerea, Sclerotinia sclerotiorum, and Erysiphe orontii. Bacterial pathogens to which resistance may be conferred include Pseudomonas syringae. Other problem organisms may potentially include nematodes, mollicutes, parasites, or herbivorous arthropods. In each case, one or more transformed transcription factor genes may provide some benefit to the plant to help prevent or overcome infestation, or be used to manipulate any of the various plant responses to disease. These mechanisms by which the transcription factors work could include increasing surface waxes or oils, surface thickness, or the activation of signal transduction pathways that regulate plant defense in response to attacks by herbivorous pests (including, for example, protease inhibitors). Another means to combat fungal and other pathogens is by accelerating local cell death or senescence, mechanisms used to impair the spread of pathogenic microorganisms throughout a plant. For instance, the best known example of accelerated cell death is the resistance gene-mediated hypersensitive response, which causes localized cell death at an infection site and initiates a systemic defense response. Because many defenses, signaling molecules, and signal transduction pathways are common to defense against different pathogens and pests, such as fungal, bacterial, oomycete, nematode, and insect, transcription factors that are implicated in defense responses against the fungal pathogens tested may also function in defense against other pathogens and pests. These transcription factors include, for example, G28, G1792, G1880, G1919, G1950 (improved resistance or tolerance to Botrytis), G1047, G1792 (improved resistance or tolerance to Fusarium), G19, G28, G409, G1266, G1363, G1792 (improved resistance or tolerance to Erysiphe), G545 (improved resistance or tolerance to Pseudomonas), G28, G1927 (improved resistance or tolerance to Sclerotinia), and their equivalogs.

Growth regulator: sugar sensing. In addition to their important role as an energy source and structural component of the plant cell, sugars are central regulatory molecules that control several aspects of plant physiology, metabolism and development (Hsieh et al. (1998) Proc. Natl. Acad. Sci. 95: 13965-13970). It is thought that this control is achieved by regulating gene expression and, in higher plants, sugars have been shown to repress or activate plant genes involved in many essential processes such as photosynthesis, glyoxylate metabolism, respiration, starch and sucrose synthesis and degradation, pathogen response, wounding response, cell cycle regulation, pigmentation, flowering and senescence. The mechanisms by which sugars control gene expression are not understood.

Because sugars are important signaling molecules, the ability to control either the concentration of a signaling sugar or how the plant perceives or responds to a signaling sugar could be used to control plant development, physiology or metabolism. For example, the flux of sucrose (a disaccharide sugar used for systemically transporting carbon and energy in most plants) has been shown to affect gene expression and alter storage compound accumulation in seeds. Manipulation of the sucrose signaling pathway in seeds may therefore cause seeds to have more protein, oil or carbohydrate, depending on the type of manipulation. Similarly, in tubers, sucrose is converted to starch which is used as an energy store. It is thought that sugar signaling pathways may partially determine the levels of starch synthesized in the tubers. The manipulation of sugar signaling in tubers could lead to tubers with a higher starch content.

Thus, the presently disclosed transcription factor genes that manipulate the sugar signal transduction pathway, including G241, G254, G567, G680, G912, G1804, G481, G867, G1225, along with their equivalogs, may lead to altered gene expression to produce plants with desirable traits. In particular, manipulation of sugar signal transduction pathways could be used to alter source-sink relationships in seeds, tubers, roots and other storage organs leading to increase in yield.

Growth regulator: C/N sensing. Nitrogen and carbon metabolism are tightly linked in almost every biochemical pathway in the plant. Carbon metabolites regulate genes involved in N acquisition and metabolism, and are known to affect germination and the expression of photosynthetic genes (Coruzzi et al. (2001) Plant Physiol. 125: 61-64) and hence growth. Early studies on nitrate reductase (R) in 1976 showed that NR activity could be affected by Glc/Suc (Crawford (1995) Plant Cell 7: 859-886; Daniel-Vedele et al. (1996) CR Acad Sci Paris 319: 961-968). Those observations were supported by later experiments that showed sugars induce NR mRNA in dark-adapted, green seedlings (Cheng C L, et al. (1992) Proc Natl Acad Sci USA 89: 1861-1864). C and N may have antagonistic relationships as signaling molecules; light induction of NR activity and mRNA levels can be mimicked by C metabolites and N-metabolites cause repression of NR induction in tobacco (Vincentz et al. (1992) Plant J 3: 315-324). Gene regulation by C/N status has been demonstrated for a number of N-metabolic genes (Stitt (1999) Curr. Opin. Plant. Biol. 2: 178-186); Coruzzi et al. (2001) supra). Thus, transcription factor genes that affect C/N sensing, such as G1816, can be used to alter or improve germination and growth under nitrogen-limiting conditions.

Flowering time: early and late flowering. Presently disclosed transcription factor genes that accelerate flowering, which include G157, G180, G183, G485, G490, G590, G789, G1225, G1494, G1820, G1841, G1842, G1843, G1946, G2010, G2144, G2295, G2347, G2509, and their functional equivalogs, could have valuable applications in such programs, since they allow much faster generation times. In a number of species, for example, broccoli, cauliflower, where the reproductive parts of the plants constitute the crop and the vegetative tissues are discarded, it would be advantageous to accelerate time to flowering. Accelerating flowering could shorten crop and tree breeding programs. Additionally, in some instances, a faster generation time would allow additional harvests of a crop to be made within a given growing season. A number of Arabidopsis genes have already been shown to accelerate flowering when constitutively expressed. These include LEAFY, APETALA1 and CONSTANS (Mandel et al. (1995) Nature 377: 522-524; Weigel and Nilsson (1995) Nature 377:et al. 495-500; Simon et al. (1996) Nature 384: 59-62).

By regulating the expression of potential flowering using inducible promoters, flowering could be triggered by application of an inducer chemical. This would allow flowering to be synchronized across a crop and facilitate more efficient harvesting. Such inducible systems could also be used to tune the flowering of crop varieties to different latitudes. At present, species such as soybean and cotton are available as a series of maturity groups that are suitable for different latitudes on the basis of their flowering time (which is governed by day-length). A system in which flowering could be chemically controlled would allow a single high-yielding northern maturity group to be grown at any latitude. In southern regions such plants could be grown for longer periods before flowering was induced, thereby increasing yields. In more northern areas, the induction would be used to ensure that the crop flowers prior to the first winter frosts.

In a sizeable number of species, for example, root crops, where the vegetative parts of the plants constitute the crop and the reproductive tissues are discarded, it is advantageous to identify and incorporate transcription factor genes that delay or prevent flowering in order to prevent resources being diverted into reproductive development. For example, G8, G47, G157, G192, G214, G231; G361, G362, G562, G736, G748, G859, G910, G913, G971, G1051, G1052, G1357, G1452, G1478, G1804, G1895, G1945, G2007, G2133, G2155, G2838 and equivalogs, delay flowering time in transgenic plants. Extending vegetative development with presently disclosed transcription factor genes could thus bring about large increases in yields. Prevention of flowering can help maximize vegetative yields and prevent escape of genetically modified organism (GMO) pollen.

Presently disclosed transcription factors that extend flowering time have utility in engineering plants with longer-lasting flowers for the horticulture industry, and for extending the time in which the plant is fertile.

A number of the presently disclosed transcription factors may extend flowering time, and delay flower abscission, which would have utility in engineering plants with longer-lasting flowers for the horticulture industry. This would provide a significant benefit to the ornamental industry, for both cut flowers and woody plant varieties (of, for example, maize), as well as have the potential to lengthen the fertile period of a plant, which could positively impact yield and breeding programs.

General development and morphology: flower structure and inflorescence: architecture, altered flower organs, reduced fertility, multiple alterations, aerial rosettes, branching, internode distance, terminal flowers and phase change. Presently disclosed transgenic transcription factors such as G353; G354, G638; G779; G988; G1063; G1075; G1140; G1449; G1499; G2143; G2557, G2838, G2839 and their equivalogs, may be used to create plants with larger flowers or arrangements of flowers that are distinct from wild-type or non-transformed cultivars. This would likely have the most value for the ornamental horticulture industry, where larger flowers or interesting floral configurations are generally preferred and command the highest prices.

Flower structure may have advantageous or deleterious effects on fertility, and could be used, for example, to decrease fertility by the absence, reduction or screening of reproductive components. In fact, plants that overexpress a sizable number of the presently disclosed transcription factor genes e.g., G470, G779, G988, G1075, G1140, G1499, G1947, G2143, G2557 and their functional equivalogs, possess reduced fertility; flowers are infertile and fail to yield seed. These could be desirable traits, as low fertility could be exploited to prevent or minimize the escape of the pollen of genetically modified organisms (GMOs) into the environment.

The alterations in shoot architecture seen in the lines transformed with G47, G1063, G1645, G2143, and their functional equivalogs indicates that these genes and their equivalogs can be used to manipulate inflorescence branching patterns. This could influence yield and offer the potential for more effective harvesting techniques. For example, a “self pruning” mutation of tomato results in a determinate growth pattern and facilitates mechanical harvesting (Pnueli et al. (2001) Plant Cell 13(12): 2687-702).

One interesting application for manipulation of flower structure, for example, by introduced transcription factors could be in the increased production of edible flowers or flower parts, including saffron, which is derived from the stigmas of Crocus sativus.

Genes that later silique conformation in brassicates may be used to modify fruit ripening processes in brassicates and other plants, which may positively affect seed or fruit quality.

A number of the presently disclosed transcription factors may affect the timing of phase changes in plants. Since the timing or phase changes generally affects a plant's eventual size, these genes may prove beneficial by providing means for improving yield and biomass.

General development and morphology: shoot meristem and branching patterns. Several of the presently disclosed transcription factor genes, including G390 and G391, and G1794, when introduced into plants, have been shown to cause stem bifurcations in developing shoots in which the shoot meristems split to form two or three separate shoots. These transcription factors and their functional equivalogs may thus be used to manipulate branching. This would provide a unique appearance, which may be desirable in ornamental applications, and may be used to modify lateral branching for use in the forestry industry. A reduction in the formation of lateral branches could reduce knot formation. Conversely, increasing the number of lateral branches could provide utility when a plant is used as a view- or windscreen.

General development and morphology: apical dominance: The modified expression of presently disclosed transcription factors (e.g., G47, G211, G1255, G1275, G1411, G1488, G1794, G2509 and their equivalogs) that reduce apical dominance could be used in ornamental horticulture, for example, to modify plant architecture, for example, to produce a shorter, more bushy stature than wild type. The latter form would have ornamental utility as well as provide increased resistance to lodging.

General development and morphology: trichome density, development or structure. Several of the presently disclosed transcription factor genes have been used to modify trichome number, density, trichome cell fate, amount of trichome products produced by plants, or produce ectopic trichome formation. These include G225; G226, G247; G362, G370; G585, G634, G676, G682, G1332, G1452, G1995, G2826, and G2838. In most cases where the metabolic pathways are impossible to engineer, increasing trichome density or size on leaves may be the only way to increase plant productivity. Thus, by increasing trichome density, size or type, these trichome-affecting genes and their functional equivalogs would have profound utilities in molecular farming practices by making use of trichomes as a manufacturing system for complex secondary metabolites.

Trichome glands on the surface of many higher plants produce and secrete exudates that give protection from the elements and pests such as insects, microbes and herbivores. These exudates may physically immobilize insects and spores, may be insecticidal or ant-microbial or they may act as allergens or irritants to protect against herbivores. By modifying trichome location, density or activity with presently disclosed transcription factors that modify these plant characteristics, plants that are better protected and higher yielding may be the result.

A potential application for these trichome-affecting genes and their equivalogs also exists in cotton: cotton fibers are modified unicellular trichomes that develop from the outer ovule epidermis. In fact, only about 30% of these epidermal cells develop into trichomes, but all have the potential to develop a trichome fate. Trichome-affecting genes can trigger an increased number of these cells to develop as trichomes and thereby increase the yield of cotton fibers. Since the mallow family is closely related to the Brassica family, genes involved in trichome formation will likely have homologs in cotton or function in cotton.

If the effects on trichome patterning reflect a general change in heterochronic processes, trichome-affecting transcription factors or their equivalogs can be used to modify the way meristems and/or cells develop during different phases of the plant life cycle. In particular, altering the timing of phase changes could afford positive effects on yield and biomass production.

General development and morphology: stem morphology and altered vascular tissue structure. Plants transformed with transcription factor genes that modify stem morphology or lignin content may be used to affect overall plant architecture and the distribution of lignified fiber cells within the stem.

Modulating lignin content might allow the quality of wood used for furniture or construction to be improved. Lignin is energy rich; increasing lignin composition could therefore be valuable in raising the energy content of wood used for fuel. Conversely, the pulp and paper industries seek wood with a reduced lignin content. Currently, lignin must be removed in a costly process that involves the use of many polluting chemicals. Consequently, lignin is a serious barrier to efficient pulp and paper production (Tzfira et al. (1998) TIBTECH 16: 439-446; Robinson (1999) Nature Biotechnology 17: 27-30). In addition to forest biotechnology applications, changing lignin content by selectively expressing or repressing transcription factors in fruits and vegetables might increase their palatability.

Transcription factors that modify stem structure, including G47, G438, G748, G988, G1488 and their equivalogs, may also be used to achieve reduction of higher-order shoot development, resulting in significant plant architecture modification. Overexpression of the genes that encode these transcription factors in woody plants might result in trees that lack side branches, and have fewer knots in the wood. Altering branching patterns could also have applications amongst ornamental and agricultural crops. For example, applications might exist in any species where secondary shoots currently have to be removed manually, or where changes in branching pattern could increase yield or facilitate more efficient harvesting.

General development and morphology: altered root development. By modifying the structure or development of roots by transforming into a plant one or more of the presently disclosed transcription factor genes, including G225, G226, G1482, and their equivalogs, plants may be produced that have the capacity to thrive in otherwise unproductive soils. For example, grape roots extending further into rocky soils would provide greater anchorage, greater coverage with increased branching, or would remain viable in waterlogged soils, thus increasing the effective planting range of the crop and/or increasing yield and survival. It may be advantageous to manipulate a plant to produce short roots, as when a soil in which the plant will be growing is occasionally flooded, or when pathogenic fungi or disease-causing nematodes are prevalent.

General development and morphology: seed development, ripening and germination rate. A number of the presently disclosed transcription factor genes (e.g., G979) have been shown to modify seed development and germination rate, including when the seeds are in conditions normally unfavorable for germination (e.g., cold, heat or salt stress, or in the presence of ABA), and may, along with functional equivalogs, thus be used to modify and improve germination rates under adverse conditions.

General development and morphology: cell differentiation and cell proliferation. Several of the disclosed transcription factors regulate cell proliferation and/or differentiation, including G1540 and its functional equivalogs. Control of these processes could have valuable applications in plant transformation, cell culture or micro-propagation systems, as well as in control of the proliferation of particular useful tissues or cell types. Transcription factors that induce the proliferation of undifferentiated cells can be operably linked with an inducible promoter to promote the formation of callus that can be used for transformation or production of cell suspension cultures. Transcription factors that prevent cells from differentiating, such as G1540 or its equivalogs, could be used to confer stem cell identity to cultured cells. Transcription factors that promote differentiation of shoots could be used in transformation or micro-propagation systems, where regeneration of shoots from callus is currently problematic. In addition, transcription factors that regulate the differentiation of specific tissues could be used to increase the proportion of these tissues in a plant. Genes that promote the differentiation of carpel tissue could be introduced into commercial species to induce formation of increased numbers of carpels or fruits. A particular application might exist in saffron, one of the world's most expensive spices. Saffron filaments, or threads, are actually the dried stigmas of the saffron flower, Crocus sativus Linneaus. Each flower contains only three stigmas, and more than 75,000 of these flowers are needed to produce just one pound of saffron filaments. An increase in carpel number would increase the quantity of stigmatic tissue and improve yield.

General development and morphology: cell expansion. Plant growth results from a combination of cell division and cell expansion. Transcription factors may be useful in regulation of cell expansion. Altered regulation of cell expansion could affect stem length, an important agronomic characteristic. For instance, short cultivars of wheat contributed to the Green Revolution, because plants that put fewer resources into stem elongation allocate more resources into developing seed and produce higher yield. These plants are also less vulnerable to wind and rain damage. These cultivars were found to be altered in their sensitivity to gibberellins, hormones that regulate stem elongation through control of both cell expansion and cell division. Altered cell expansion in leaves could also produce novel and ornamental plant forms.

General development and morphology: phase change and floral reversion. Transcription factors that regulate phase change can modulate the developmental programs of plants and regulate developmental plasticity of the shoot meristem. In particular, these genes might be used to manipulate seasonality and influence whether plants display an annual or perennial habit.

General development and morphology: rapid development. A number of the presently disclosed transcription factor genes, including G2430, have been shown to have significant effects on plant growth rate and development. These observations have included, for example, more rapid or delayed growth and development of reproductive organs. Thus, by causing more rapid development, G2430 and its functional equivalogs would prove useful for regions with short growing seasons; other transcription factors that delay development may be useful for regions with longer growing seasons. Accelerating plant growth would also improve early yield or increase biomass at an earlier stage, when such is desirable (for example, in producing forestry products or vegetable sprouts for consumption). Transcription factors that promote faster development such as G2430 and its functional equivalogs may also be used to modify the reproductive cycle of plants.

General development and morphology: slow growth rate. A number of the presently disclosed transcription factor genes, including G652 and G1335, have been shown to have significant effects on retarding plant growth rate and development. These observations have included, for example, delayed growth and development of reproductive organs. Slow growing plants may be highly desirable to ornamental horticulturists, both for providing house plants that display little change in their appearance over time, or outdoor plants for which wild-type or rapid growth is undesirable (e.g., ornamental palm trees). Slow growth may also provide for a prolonged fruiting period, thus extending the harvesting season, particularly in regions with long growing seasons. Slow growth could also provide a prolonged period in which pollen is available for improved self- or cross-fertilization, or cross-fertilization of cultivars that normally flower over non-overlapping time periods. The latter aspect may be particularly useful to plants comprising two or more distinct grafted cultivars (e.g., fruit trees) with normally non-overlapping flowering periods.

General development and morphology: senescence. Presently disclosed transcription factor genes may be used to alter senescence responses in plants. Although leaf senescence is thought to be an evolutionary adaptation to recycle nutrients, the ability to control senescence in an agricultural setting has significant value. For example, a delay in leaf senescence in some maize hybrids is associated with a significant increase in yields and a delay of a few days in the senescence of soybean plants can have a large impact on yield. In an experimental setting, tobacco plants engineered to inhibit leaf senescence had a longer photosynthetic lifespan, and produced a 50% increase in dry weight and seed yield (Gan and Amasino (1995) Science 270: 1986-1988). Delayed flower senescence caused by overexpression of transcription factors may generate plants that retain their blossoms longer and this may be of potential interest to the ornamental horticulture industry, and delayed foliar and fruit senescence could improve post-harvest shelf-life of produce.

Premature senescence caused by, for example, G636, G1463, G1944 and their equivalogs may be used to improve a plant's response to disease and hasten fruit ripening.

Growth rate and development: lethality and necrosis. Overexpression of transcription factors, for example, G12, G24, G877, G1519 and their equivalogs that have a role in regulating cell death may be used to induce lethality in specific tissues or necrosis in response to pathogen attack. For example, if a transcription factor gene inducing lethality or necrosis was specifically active in gametes or reproductive organs, its expression in these tissues would lead to ablation and subsequent male or female sterility. Alternatively, under pathogen-regulated expression, a necrosis-inducing transcription factor can restrict the spread of a pathogen infection through a plant.

Plant size: large plants. Plants overexpressing G1073 and G1451, for example, have been shown to be larger than controls. For some ornamental plants, the ability to provide larger varieties with these genes or their equivalogs may be highly desirable. For many plants, including fruit-bearing trees, trees that are used for lumber production, or trees and shrubs that serve as view or wind screens, increased stature provides improved benefits in the forms of greater yield or improved screening. Crop species may also produce higher yields on larger cultivars, particularly those in which the vegetative portion of the plant is edible.

Plant size: large seedlings. Presently disclosed transcription factor genes, that produce large seedlings can be used to produce crops that become established faster. Large seedlings are generally hardier, less vulnerable to stress, and better able to out-compete weed species. Seedlings transformed with presently disclosed transcription factors, including G2346 and G2838, for example, have been shown to possess larger cotyledons and were more developmentally advanced than control plants. Rapid seedling development made possible by manipulating expression of these genes or their equivalogs is likely to reduce loss due to diseases particularly prevalent at the seedling stage (e.g., damping off) and is thus important for survivability of plants germinating in the field or in controlled environments.

Plant size: dwarfed plants. Presently disclosed transcription factor genes, including G24; G343, G353, G354, G362, G370; G1008, G1277, G1543, G1794, G1958 and their equivalogs, for example, that can be used to decrease plant stature are likely to produce plants that are more resistant to damage by wind and rain, have improved lodging resistance, or more resistant to heat or low humidity or water deficit. Dwarf plants are also of significant interest to the ornamental horticulture industry, and particularly for home garden applications for which space availability may be limited.

Plant size: fruit size and number. Introduction of presently disclosed transcription factor genes that affect fruit size will have desirable impacts on fruit size and number, which may comprise increases in yield for fruit crops, or reduced fruit yield, such as when vegetative growth is preferred (e.g., with bushy ornamentals, or where fruit is undesirable, as with ornamental olive trees).

Leaf morphology: dark leaves. Color-affecting components in leaves include chlorophylls (generally green), anthocyanins (generally red to blue) and carotenoids (generally yellow to red). Transcription factor genes that increase these pigments in leaves, including G674, G912, G1063, G1357, G1452, G1482, G1499, G1792, G1863, G1888, G2143, G2557, G2838 and their equivalogs, may positively affect a plant's value to the ornamental horticulture industry. Variegated varieties, in particular, would show improved contrast. Other uses that result from overexpression of transcription factor genes include improvements in the nutritional value of foodstuffs. For example, lutein is an important nutraceutical; lutein-rich diets have been shown to help prevent age-related macular degeneration (ARMD), the leading cause of blindness in elderly people. Consumption of dark green leafy vegetables has been shown in clinical studies to reduce the risk of ARMD.

Enhanced chlorophyll and carotenoid levels could also improve yield in crop plants. Lutein, like other xanthophylls such as zeaxanthin and violaxanthin, is an essential component in the protection of the plant against the damaging effects of excessive light. Specifically, lutein contributes, directly or indirectly, to the rapid rise of non-photochemical quenching in plants exposed to high light. Crop plants engineered to contain higher levels of lutein could therefore have improved photo-protection, leading to less oxidative damage and better growth under high light (e.g., during long summer days, or at higher altitudes or lower latitudes than those at which a non-transformed plant would survive). Additionally, elevated chlorophyll levels increases photosynthetic capacity.

Leaf morphology: changes in leaf shape. Presently disclosed transcription factors produce marked and diverse effects on leaf development and shape. The transcription factors include G211, G353, G674, G736, G1063, G1146, G1357, G1452, G1494, G1543, G1863, G2143, G2144, and their equivalogs. At early stages of growth, transgenic seedlings have developed narrow, upward pointing leaves with long petioles, possibly indicating a disruption in circadian-clock controlled processes or nyctinastic movements. Other transcription factor genes can be used to alter leaf shape in a significant manner from wild type, some of which may find use in ornamental applications.

Leaf morphology: altered leaf size. Large leaves, such as those produced in plants overexpressing G189, G1451, G2430 and their functional equivalogs, generally increase plant biomass. This provides benefit for crops where the vegetative portion of the plant is the marketable portion.

Leaf morphology: light green and variegated leaves. Transcription factor genes such as G635, G1494, G2144 and their equivalogs that provide an altered appearance may positively affect a plant's value to the ornamental horticulture industry.

Leaf morphology: glossy leaves. Transcription factor genes such as G30, G1792, G2583 and their equivalogs that induce the formation of glossy leaves generally do so by elevating levels of epidermal wax. Thus, the genes could be used to engineer changes in the composition and amount of leaf surface components, including waxes. The ability to manipulate wax composition, amount, or distribution could modify plant tolerance to drought and low humidity, or resistance to insects or pathogens. Additionally, wax may be a valuable commodity in some species, and altering its accumulation and/or composition could enhance yield.

Seed morphology: altered seed coloration. Presently disclosed transcription factor genes, including G156, G2105, G2085 have also been used to modify seed color, which, along with the equivalogs of these genes, could provide added appeal to seeds or seed products.

Seed morphology: altered seed size and shape. The introduction of presently disclosed transcription factor genes into plants that increase (e.g., G450; G584; G1255; G2085; G2105; G2114) or decrease (e.g., G1040). the size of seeds may have a significant impact on yield and appearance, particularly when the product is the seed itself (e.g., in the case of grains, legumes, nuts, etc.). Seed size, in addition to seed coat integrity, thickness and permeability, seed water content and a number of other components including antioxidants and oligosaccharides, also affects affect seed longevity in storage, with larger seeds often being more desirable for prolonged storage.

Transcription factor genes that alter seed shape, including G1040, G1062, G1255 and their equivalogs may have both ornamental applications and improve or broaden the appeal of seed products.

Leaf biochemistry: increased leaf wax. Overexpression of transcription factors genes, including G975, G1792 and G2085 and their equivalogs, which results in increased leaf wax could be used to manipulate wax composition, amount, or distribution. These transcription factors can improve yield in those plants and crops from which wax is a valuable product. The genes may also be used to modify plant tolerance to drought and/or low humidity or resistance to insects, as well as plant appearance (glossy leaves). The effect of increased wax deposition on leaves of a plant like may improve water use efficiency. Manipulation of these genes may reduce the wax coating on sunflower seeds; this wax fouls the oil extraction system during sunflower seed processing for oil. For the latter purpose or any other where wax reduction is valuable, antisense or cosuppression of the transcription factor genes in a tissue-specific manner would be valuable.

Leaf biochemistry: leaf prenyl lipids, including tocopherol. Prenyl lipids play a role in anchoring proteins in membranes or membranous organelles. Thus modifying the prenyl lipid content of seeds and leaves could affect membrane integrity and function. One important group of prenyl lipids, the tocopherols, have both anti-oxidant and vitamin E activity. A number of presently disclosed transcription factor genes, including G214, G652, G748, G987, G1543, and G2509, have been shown to modify the tocopherol composition of leaves in plants, and these genes and their equivalogs may thus be used to alter prenyl lipid content of leaves.

Leaf biochemistry: increased leaf insoluble sugars. Overexpression of a number of presently disclosed transcription factors, including G211, resulted in plants with altered leaf insoluble sugar content. This transcription factor and its equivalogs that alter plant cell wall composition have several potential applications including altering food digestibility, plant tensile strength, wood quality, pathogen resistance and in pulp production. In particular, hemicellulose is not desirable in paper pulps because of its lack of strength compared with cellulose. Thus modulating the amounts of cellulose vs. hemicellulose in the plant cell wall is desirable for the paper/lumber industry. Increasing the insoluble carbohydrate content in various fruits, vegetables, and other edible consumer products will result in enhanced fiber content. Increased fiber content would not only provide health benefits in food products, but might also increase digestibility of forage crops. In addition, the hemicellulose and pectin content of fruits and berries affects the quality of jam and catsup made from them. Changes in hemicellulose and pectin content could result in a superior consumer product.

Leaf biochemistry: increased leaf anthocyanin. Several presently disclosed transcription factor genes may be used to alter anthocyanin production in numerous plant species. Expression of presently disclosed transcription factor genes that increase flavonoid production in plants, including anthocyanins and condensed tannins, may be used to alter in pigment production for horticultural purposes, and possibly increasing stress resistance. G362, G663, G1482 and G1888 or their equivalogs, for example, could be used to alter anthocyanin production or accumulation. A number of flavonoids have been shown to have antimicrobial activity and could be used to engineer pathogen resistance. Several flavonoid compounds have health promoting effects such as inhibition of tumor growth, prevention of bone loss and prevention of the oxidation of lipids. Increased levels of condensed tannins, in forage legumes would be an important agronomic trait because they prevent pasture bloat by collapsing protein foams within the rumen. For a review on the utilities of flavonoids and their derivatives, refer to Dixon et al. (1999) Trends Plant Sci. 4: 394-400.

Leaf and seed biochemistry: altered fatty acid content. A number of the presently disclosed transcription factor genes have been shown to alter the fatty acid composition in plants, and seeds and leaves in particular. This modification suggests several utilities, including improving the nutritional value of seeds or whole plants. Dietary fatty acids ratios have been shown to have an effect on, for example, bone integrity and remodeling (see, for example, Weiler (2000) Pediatr. Res. 47:5 692-697). The ratio of dietary fatty acids may alter the precursor pools of long-chain polyunsaturated fatty acids that serve as precursors for prostaglandin synthesis. In mammalian connective tissue, prostaglandins serve as important signals regulating the balance between resorption and formation in bone and cartilage. Thus dietary fatty acid ratios altered in seeds may affect the etiology and outcome of bone loss.

Transcription factors that reduce leaf fatty acids, for example, 16:3 fatty acids, may be used to control thylakoid membrane development, including proplastid to chloroplast development. The genes that encode these transcription factors might thus be useful for controlling the transition from proplastid to chromoplast in fruits and vegetables. It may also be desirable to change the expression of these genes to prevent cotyledon greening in Brassica napus or B. campestris to avoid green oil due to early frost.

A number of transcription factor genes are involved in mediating an aspect of the regulatory response to temperature. These genes may be used to alter the expression of desaturases that lead to production of 18:3 and 16:3 fatty acids, the balance of which affects membrane fluidity and mitigates damage to cell membranes and photosynthetic structures at high and low temperatures.

Seed biochemistry: modified seed oil and fatty acid content. The composition of seeds, particularly with respect to seed oil amounts and/or composition, is very important for the nutritional and caloric value and production of various food and feed products. Several of the presently disclosed transcription factor genes in seed lipid saturation that alter seed oil content could be used to improve the heat stability of oils or to improve the nutritional quality of seed oil, by, for example, reducing the number of calories in seed by decreasing oil or fatty acid content (e.g., G180; G192; G241; G1229; G1323; G1543), increasing the number of calories in animal feeds by increasing oil or fatty acid content (e.g. G162; G291; G427; G590; G598; G629, G715; G849; G1198, G1471; G1526; G1640; G1646, G1750; G1777; G1793; G1838; G1902; G1946; G1948; G2123; G2138; G2830), altering seed oil content (G504; G509; G519; G561; G567; G892; G961; G974; G1143; G1226; G1451; G1478; G1496; G1672; G1677; G1765; G2509; G2343), or altering the ratio of saturated to unsaturated lipids comprising the oils (e.g. G869; G1417; G2192).

Seed biochemistry: modified seed protein content. As with seed oils, the composition of seeds, particularly with respect to protein amounts and/or composition, is very important for the nutritional value and production of various food and feed products. A number of the presently disclosed transcription factor genes modify the protein concentrations in seeds, including G162; G226; G1323; G1419; G1818, which increase seed protein, G427; G1777; G1903; G1946, which decrease seed protein, and G162; G241; G509; G567; G597; G849; G892; G988; G1478; G1634; G1637; G1652; G1677; G1820; G1958; G2509; G2117; G2509, which alter seed protein content, would provide nutritional benefits, and may be used to prolong storage, increase seed pest or disease resistance, or modify germination rates.

Seed biochemistry: seed prenyl lipids. Prenyl lipids play a role in anchoring proteins in membranes or membranous organelles. Thus, modifying the prenyl lipid content of seeds and leaves could affect membrane integrity and function. A number of presently disclosed transcription factor genes have been shown to modify the tocopherol composition of plants. α-Tocopherol is better known as vitamin E. Tocopherols such as α- and γ-tocopherol both have anti-oxidant activity.

Seed biochemistry: seed glucosinolates. A number of glucosinolates have been shown to have anti-cancer activity; thus, increasing the levels or composition of these compounds by introducing several of the presently disclosed transcription factors, including G484 and G2340, can have a beneficial effect on human diet.

Glucosinolates are undesirable components of the oilseeds used in animal feed since they produce toxic effects. Low-glucosinolate varieties of canola, for example, have been developed to combat this problem. Glucosinolates form part of a plant's natural defense against insects. Modification of glucosinolate composition or quantity by introducing transcription factors that affect these characteristics can therefore afford increased protection from herbivores. Furthermore, in edible crops, tissue specific promoters can be used to ensure that these compounds accumulate specifically in tissues, such as the epidermis, which are not taken for consumption.

Seed biochemistry: increased seed anthocyanin. Several presently disclosed transcription factor genes may be used to alter anthocyanin production in the seeds of plants. As with leaf anthocyanins, expression of presently disclosed transcription factor genes that increase flavonoid (anthocyanins and condensed tannins) production in seeds, including G663 and its equivalogs, may be used to alter in pigment production for horticultural purposes, and possibly increasing stress resistance, antimicrobial activity and health promoting effects such as inhibition of tumor growth, prevention of bone loss and prevention of the oxidation of lipids.

Leaf and seed biochemistry: production of seed and leaf phytosterols: Presently disclosed transcription factor genes that modify levels of phytosterols in plants may have at least two utilities. First, phytosterols are an important source of precursors for the manufacture of human steroid hormones. Thus, regulation of transcription factor expression or activity could lead to elevated levels of important human steroid precursors for steroid semi-synthesis. For example, transcription factors that cause elevated levels of campesterol in leaves, or sitosterols and stigmasterols in seed crops, would be useful for this purpose. Phytosterols and their hydrogenated derivatives phytostanols also have proven cholesterol-lowering properties, and transcription factor genes that modify the expression of these compounds in plants would thus provide health benefits.

Root biochemistry: increased root anthocyanin. Presently disclosed transcription factor genes, including G663, may be used to alter anthocyanin production in the root of plants. As described above for seed anthocyanins, expression of presently disclosed transcription factor genes that increase flavonoid (anthocyanins and condensed tannins) production in seeds, including G663 and its equivalogs, may be used to alter in pigment production for horticultural purposes, and possibly increasing stress resistance, antimicrobial activity and health promoting effects such as inhibition of tumor growth, prevention of bone loss and prevention of the oxidation of lipids.

Light response/shade avoidance: altered cotyledon, hypocotyl, petiole development, altered leaf orientation, constitutive photomorphogenesis, photomorphogenesis in low light. Presently disclosed transcription factor genes, including G183; G354; G1322; G1331; G1488; G1494; G1794; G2144; and G2555, that modify a plant's response to light may be useful for modifying plant growth or development, for example, photomorphogenesis in poor light, or accelerating flowering time in response to various light intensities, quality or duration to which a non-transformed plant would not similarly respond. Examples of such responses that have been demonstrated include leaf number and arrangement, and early flower bud appearances. Elimination of shading responses may lead to increased planting densities with subsequent yield enhancement. As these genes may also alter plant architecture, they may find use in the ornamental horticulture industry.

Pigment: increased anthocyanin level in various plant organs and tissues. In addition to seed, leaves and roots, as mentioned above, several presently disclosed transcription factor genes can be used to alter anthocyanin levels in one or more tissues. The potential utilities of these genes include alterations in pigment production for horticultural purposes, and possibly increasing stress resistance, antimicrobial activity and health promoting effects such as inhibition of tumor growth, prevention of bone loss and prevention of the oxidation of lipids.

Miscellaneous biochemistry: diterpenes in leaves and other plant parts. Depending on the plant species, varying amounts of diverse secondary biochemicals (often lipophilic terpenes) are produced and exuded or volatilized by trichomes. These exotic secondary biochemicals, which are relatively easy to extract because they are on the surface of the leaf, have been widely used in such products as flavors and aromas, drugs, pesticides and cosmetics. Thus, the overexpression of genes that are used to produce diterpenes in plants may be accomplished by introducing transcription factor genes that induce said overexpression. One class of secondary metabolites, the diterpenes, can effect several biological systems such as tumor progression, prostaglandin synthesis and tissue inflammation. In addition, diterpenes can act as insect pheromones, termite allomones, and can exhibit neurotoxic, cytotoxic and antimitotic activities. As a result of this functional diversity, diterpenes have been the target of research several pharmaceutical ventures. In most cases where the metabolic pathways are impossible to engineer, increasing trichome density or size on leaves may be the only way to increase plant productivity.

Miscellaneous biochemistry: production of miscellaneous secondary metabolites. Microarray data suggests that flux through the aromatic amino acid biosynthetic pathways and primary and secondary metabolite biosynthetic pathways are up-regulated. Presently disclosed transcription factors have been shown to be involved in regulating alkaloid biosynthesis, in part by up-regulating the enzymes indole-3-glycerol phosphatase and strictosidine synthase. Phenylalanine ammonia lyase, chalcone synthase and trans-cinnamate mono-oxygenase are also induced, and are involved in phenylpropenoid biosynthesis.

Antisense and Co-Suppression

In addition to expression of the nucleic acids of the invention as gene replacement or plant phenotype modification nucleic acids, the nucleic acids are also useful for sense and anti-sense suppression of expression, e.g., to down-regulate expression of a nucleic acid of the invention, e.g., as a further mechanism for modulating plant phenotype. That is, the nucleic acids of the invention, or subsequences or anti-sense sequences thereof, can be used to block expression of naturally occurring homologous nucleic acids. A variety of sense and anti-sense technologies are known in the art, e.g., as set forth in Lichtenstein and Nellen (1997) Antisense Technology: A Practical Approach IRL Press at Oxford University Press, Oxford, U.K. Antisense regulation is also described in Crowley et al. (1985) Cell 43: 633-641; Rosenberg et al. (1985) Nature 313: 703-706; Preiss et al. (1985) Nature 313: 27-32; Melton (1985) Proc. Natl. Acad. Sci. 82: 144-148; Izant and Weintraub (1985) Science 229: 345-352; and Kim and Wold (1985) Cell 42: 129-138. Additional methods for antisense regulation are known in the art. Antisense regulation has been used to reduce or inhibit expression of plant genes in, for example in European Patent Publication No. 271988. Antisense RNA may be used to reduce gene expression to produce a visible or biochemical phenotypic change in a plant (Smith et al. (1988) Nature, 334: 724-726; Smith et al. (1990) Plant Mol. Biol. 14: 369-379). In general, sense or anti-sense sequences are introduced into a cell, where they are optionally amplified, e.g., by transcription. Such sequences include both simple oligonucleotide sequences and catalytic sequences such as ribozymes.

For example, a reduction or elimination of expression (i.e., a “knock-out”) of a transcription factor or transcription factor homolog polypeptide in a transgenic plant, e.g., to modify a plant trait, can be obtained by introducing an antisense construct corresponding to the polypeptide of interest as a cDNA. For antisense suppression, the transcription factor or homolog cDNA is arranged in reverse orientation (with respect to the coding sequence) relative to the promoter sequence in the expression vector. The introduced sequence need not be the full length cDNA or gene, and need not be identical to the cDNA or gene found in the plant type to be transformed. Typically, the antisense sequence need only be capable of hybridizing to the target gene or RNA of interest. Thus, where the introduced sequence is of shorter length, a higher degree of homology to the endogenous transcription factor sequence will be needed for effective antisense suppression. While antisense sequences of various lengths can be utilized, preferably, the introduced antisense sequence in the vector will be at least 30 nucleotides in length, and improved antisense suppression will typically be observed as the length of the antisense sequence increases. Preferably, the length of the antisense sequence in the vector will be greater than 100 nucleotides. Transcription of an antisense construct as described results in the production of RNA molecules that are the reverse complement of mRNA molecules transcribed from the endogenous transcription factor gene in the plant cell.

Suppression of endogenous transcription factor gene expression can also be achieved using a ribozyme. Ribozymes are RNA molecules that possess highly specific endoribonuclease activity. The production and use of ribozymes are disclosed in U.S. Pat. No. 4,987,071 and U.S. Pat. No. 5,543,508. Synthetic ribozyme sequences including antisense RNAs can be used to confer RNA cleaving activity on the antisense RNA, such that endogenous mRNA molecules that hybridize to the antisense RNA are cleaved, which in turn leads to an enhanced antisense inhibition of endogenous gene expression.

Vectors in which RNA encoded by a transcription factor or transcription factor homolog cDNA is over-expressed can also be used to obtain co-suppression of a corresponding endogenous gene, e.g., in the manner described in U.S. Pat. No. 5,231,020 to Jorgensen. Such co-suppression (also termed sense suppression) does not require that the entire transcription factor cDNA be introduced into the plant cells, nor does it require that the introduced sequence be exactly identical to the endogenous transcription factor gene of interest. However, as with antisense suppression, the suppressive efficiency will be enhanced as specificity of hybridization is increased, e.g., as the introduced sequence is lengthened, and/or as the sequence similarity between the introduced sequence and the endogenous transcription factor gene is increased.

Vectors expressing an untranslatable form of the transcription factor mRNA, e.g., sequences comprising one or more stop codon, or nonsense mutation) can also be used to suppress expression of an endogenous transcription factor, thereby reducing or eliminating its activity and modifying one or more traits. Methods for producing such constructs are described in U.S. Pat. No. 5,583,021. Preferably, such constructs are made by introducing a premature stop codon into the transcription factor gene. Alternatively, a plant trait can be modified by gene silencing using double-strand RNA (Sharp (1999) Genes and Development 13: 139-141). Another method for abolishing the expression of a gene is by insertion mutagenesis using the T-DNA of Agrobacterium tumefaciens. After generating the insertion mutants, the mutants can be screened to identify those containing the insertion in a transcription factor or transcription factor homolog gene. Plants containing a single transgene insertion event at the desired gene can be crossed to generate homozygous plants for the mutation. Such methods are well known to those of skill in the art (See for example Koncz et al. (1992) Methods in Arabidopsis Research, World Scientific Publishing Co. Pte. Ltd., River Edge, N.J.).

Alternatively, a plant phenotype can be altered by eliminating an endogenous gene, such as a transcription factor or transcription factor homolog, e.g., by homologous recombination (Kempin et al. (1997) Nature 389: 802-803).

A plant trait can also be modified by using the Cre-lox system (for example, as described in U.S. Pat. No. 5,658,772). A plant genome can be modified to include first and second lox sites that are then contacted with a Cre recombinase. If the lox sites are in the same orientation, the intervening DNA sequence between the two sites is excised. If the lox sites are in the opposite orientation, the intervening sequence is inverted.

The polynucleotides and polypeptides of this invention can also be expressed in a plant in the absence of an expression cassette by manipulating the activity or expression level of the endogenous gene by other means, such as, for example, by ectopically expressing a gene by T-DNA activation tagging (Ichikawa et al. (1997) Nature 390 698-701; Kakimoto et al. (1996) Science 274: 982-985). This method entails transforming a plant with a gene tag containing multiple transcriptional enhancers and once the tag has inserted into the genome, expression of a flanking gene coding sequence becomes deregulated. In another example, the transcriptional machinery in a plant can be modified so as to increase transcription levels of a polynucleotide of the invention (See, e.g., PCT Publications WO 96/06166 and WO 98/53057 which describe the modification of the DNA-binding specificity of zinc finger proteins by changing particular amino acids in the DNA-binding motif).

The transgenic plant can also include the machinery necessary for expressing or altering the activity of a polypeptide encoded by an endogenous gene, for example, by altering the phosphorylation state of the polypeptide to maintain it in an activated state.

Transgenic plants (or plant cells, or plant explants, or plant tissues) incorporating the polynucleotides of the invention and/or expressing the polypeptides of the invention can be produced by a variety of well established techniques as described above. Following construction of a vector, most typically an expression cassette, including a polynucleotide, e.g., encoding a transcription factor or transcription factor homolog, of the invention, standard techniques can be used to introduce the polynucleotide into a plant, a plant cell, a plant explant or a plant tissue of interest. Optionally, the plant cell, explant or tissue can be regenerated to produce a transgenic plant.

The plant can be any higher plant, including gymnosperms, monocotyledonous and dicotyledenous plants. Suitable protocols are available for Leguminosae (alfalfa, soybean, clover, etc.), Umbelliferae (carrot, celery, parsnip), Cruciferae (cabbage, radish, rapeseed, broccoli, etc.), Curcurbitaceae (melons and cucumber), Gramineae (wheat, corn, rice, barley, millet, etc.), Solanaceae (potato, tomato, tobacco, peppers, etc.), and various other crops. See protocols described in Ammirato et al., Eds., (1984) Handbook of Plant Cell Culture—Crop Species, Macmillan Publ. Co., New York, N.Y.; Shimamoto et al. (1989) Nature 338: 274-276; Fromm et al. (1990) Bio/Technol. 8: 833-839; and Vasil et al. (1990) Bio/Technol. 8: 429-434.

Transformation and regeneration of both monocotyledonous and dicotyledonous plant cells is now routine, and the selection of the most appropriate transformation technique will be determined by the practitioner. The choice of method will vary with the type of plant to be transformed; those skilled in the art will recognize the suitability of particular methods for given plant types. Suitable methods can include, but are not limited to: electroporation of plant protoplasts; liposome-mediated transformation; polyethylene glycol (PEG) mediated transformation; transformation using viruses; micro-injection of plant cells; micro-projectile bombardment of plant cells; vacuum infiltration; and Agrobacterium tumefaciens mediated transformation. Transformation means introducing a nucleotide sequence into a plant in a manner to cause stable or transient expression of the sequence.

Successful examples of the modification of plant characteristics by transformation with cloned sequences which serve to illustrate the current knowledge in this field of technology, and which are herein incorporated by reference, include: U.S. Pat. Nos. 5,571,706; 5,677,175; 5,510,471; 5,750,386; 5,597,945; 5,589,615; 5,750,871; 5,268,526; 5,780,708; 5,538,880; 5,773,269; 5,736,369 and 5,610,042.

Following transformation, plants are preferably selected using a dominant selectable marker incorporated into the transformation vector. Typically, such a marker will confer antibiotic or herbicide resistance on the transformed plants, and selection of transformants can be accomplished by exposing the plants to appropriate concentrations of the antibiotic or herbicide.

After transformed plants are selected and grown to maturity, those plants showing a modified trait are identified. The modified trait can be any of those traits described above. Additionally, to confirm that the modified trait is due to changes in expression levels or activity of the polypeptide or polynucleotide of the invention can be determined by analyzing mRNA expression using Northern blots, RT-PCR or microarrays, or protein expression using immunoblots or Western blots or gel shift assays.

Integrated Systems—Sequence Identity

Additionally, the present invention may be an integrated system, computer or computer readable medium that comprises an instruction set for determining the identity of one or more sequences in a database. In addition, the instruction set can be used to generate or identify sequences that meet any specified criteria. Furthermore, the instruction set may be used to associate or link certain functional benefits, such improved characteristics, with one or more identified sequence.

For example, the instruction set can include, e.g., a sequence comparison or other alignment program, e.g., an available program such as, for example, the Wisconsin Package Version 10.0, such as BLAST, FASTA, PILEUP, FINDPATTERNS or the like (GCG, Madison, Wis.). Public sequence databases such as GenBank, EMBL, Swiss-Prot and PIR or private sequence databases such as PHYTOSEQ sequence database (Incyte Genomics, Palo Alto, Calif.) can be searched.

Alignment of sequences for comparison can be conducted by the local homology algorithm of Smith and Waterman (1981) Adv. Appl. Math. 2: 482-489, by the homology alignment algorithm of Needleman and Wunsch (1970) J. Mol. Biol. 48: 443-453, by the search for similarity method of Pearson and Lipman (1988) Proc. Natl. Acad. Sci. 85: 2444-2448, by computerized implementations of these algorithms. After alignment, sequence comparisons between two (or more) polynucleotides or polypeptides are typically performed by comparing sequences of the two sequences over a comparison window to identify and compare local regions of sequence similarity. The comparison window can be a segment of at least about 20 contiguous positions, usually about 50 to about 200, more usually about 100 to about 150 contiguous positions. A description of the method is provided in Ausubel et al. supra.

A variety of methods for determining sequence relationships can be used, including manual alignment and computer assisted sequence alignment and analysis. This later approach is a preferred approach in the present invention, due to the increased throughput afforded by computer assisted methods. As noted above, a variety of computer programs for performing sequence alignment are available, or can be produced by one of skill.

One example algorithm that is suitable for determining percent sequence identity and sequence similarity is the BLAST algorithm, which is described in Altschul et al. (1990) J. Mol. Biol. 215: 403-410. Software for performing BLAST analyses is publicly available, e.g., through the National Library of Medicine's National Center for Biotechnology Information (ncbi.nlm.nih; see at world wide web (www) National Institutes of Health US government (gov) website). This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al. supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are then extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always >0) and N (penalty score for mismatching residues; always <0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) of 10, a cutoff of 100, M=5, N=−4, and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff and Henikoff (1992) Proc. Natl. Acad. Sci. 89: 10915-10919). Unless otherwise indicated, “sequence identity” here refers to the % sequence identity generated from a tblastx using the NCBI version of the algorithm at the default settings using gapped alignments with the filter “off” (see, for example, NIH NLM NCBI website at ncbi.nlm.nih, supra).

In addition to calculating percent sequence identity, the BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g. Karlin and Altschul (1993) Proc. Natl. Acad. Sci. 90: 5873-5787). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a nucleic acid is considered similar to a reference sequence (and, therefore, in this context, homologous) if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.1, or less than about 0.01, and or even less than about 0.001. An additional example of a useful sequence alignment algorithm is PILEUP. PILEUP creates a multiple sequence alignment from a group of related sequences using progressive, pairwise alignments. The program can align, e.g., up to 300 sequences of a maximum length of 5,000 letters.

The integrated system, or computer typically includes a user input interface allowing a user to selectively view one or more sequence records corresponding to the one or more character strings, as well as an instruction set which aligns the one or more character strings with each other or with an additional character string to identify one or more region of sequence similarity. The system may include a link of one or more character strings with a particular phenotype or gene function. Typically, the system includes a user readable output element that displays an alignment produced by the alignment instruction set.

The methods of this invention can be implemented in a localized or distributed computing environment. In a distributed environment, the methods may implemented on a single computer comprising multiple processors or on a multiplicity of computers. The computers can be linked, e.g. through a common bus, but more preferably the computer(s) are nodes on a network. The network can be a generalized or a dedicated local or wide-area network and, in certain preferred embodiments, the computers may be components of an intra-net or an internet.

Thus, the invention provides methods for identifying a sequence similar or homologous to one or more polynucleotides as noted herein, or one or more target polypeptides encoded by the polynucleotides, or otherwise noted herein and may include linking or associating a given plant phenotype or gene function with a sequence. In the methods, a sequence database is provided (locally or across an inter or intra net) and a query is made against the sequence database using the relevant sequences herein and associated plant phenotypes or gene functions.

Any sequence herein can be entered into the database, before or after querying the database. This provides for both expansion of the database and, if done before the querying step, for insertion of control sequences into the database. The control sequences can be detected by the query to ensure the general integrity of both the database and the query. As noted, the query can be performed using a web browser based interface. For example, the database can be a centralized public database such as those noted herein, and the querying can be done from a remote terminal or computer across an internet or intranet.

Any sequence herein can be used to identify a similar, homologous, paralogous, or orthologous sequence in another plant. This provides means for identifying endogenous sequences in other plants that may be useful to alter a trait of progeny plants, which results from crossing two plants of different strain. For example, sequences that encode an ortholog of any of the sequences herein that naturally occur in a plant with a desired trait can be identified using the sequences disclosed herein. The plant is then crossed with a second plant of the same species but which does not have the desired trait to produce progeny which can then be used in further crossing experiments to produce the desired trait in the second plant. Therefore the resulting progeny plant contains no transgenes; expression of the endogenous sequence may also be regulated by treatment with a particular chemical or other means, such as EMR. Some examples of such compounds well known in the art include: ethylene; cytokinins; phenolic compounds, which stimulate the transcription of the genes needed for infection; specific monosaccharides and acidic environments which potentiate vir gene induction; acidic polysaccharides which induce one or more chromosomal genes; and opines; other mechanisms include light or dark treatment (for a review of examples of such treatments, see, Winans (1992) Microbiol. Rev. 56: 12-31; Eyal et al. (1992) Plant Mol. Biol. 19: 589-599; Chrispeels et al. (2000) Plant Mol. Biol. 42: 279-290; Piazza et al. (2002) Plant Physiol. 128: 1077-1086).

Table 7 lists sequences discovered to be orthologous to a number of representative transcription factors of the present invention. The column headings include the transcription factors listed by SEQ ID NO; corresponding Gene ID (GID) numbers; the species from which the orthologs to the transcription factors are derived; the type of sequence (i.e., DNA or protein) discovered to be orthologous to the transcription factors; and the SEQ ID NO of the orthologs, the latter corresponding to the ortholog SEQ ID NOs listed in the Sequence Listing.

TABLE 7 Orthologs of Representative Arabidopsis Transcription Factor Genes SEQ ID NO: of Nucleotide SEQ ID NO: GID NO of Encoding of Ortholog Sequence type Orthologous Orthologous or Nucleotide used for Arabidopsis Arabidopsis Encoding Ortholog Species from Which determination Transcription Transcription Ortholog GID NO Ortholog is Derived (DNA or Protein) Factor Factor 459 Glycine max DNA G8 1 460 Glycine max DNA G8 1 461 Glycine max DNA G8 1 462 Glycine max DNA G8 1 463 Oryza sativa DNA G8 1 464 Zea mays DNA G8 1 465 Zea mays DNA G8 1 466 Zea mays DNA G8 1 467 Oryza sativa PRT G8 1 468 Glycine max DNA G19 3 469 Glycine max DNA G19 3 470 Glycine max DNA G19 3 471 Glycine max DNA G19 3 472 Oryza sativa DNA G19 3 473 Oryza sativa DNA G19 3 474 Oryza sativa DNA G19 3 475 Zea mays DNA G19 3 476 Zea mays DNA G19 3 477 Glycine max DNA G22 5 478 Glycine max DNA G22 5 479 Glycine max DNA G24 7 480 Glycine max DNA G24 7 481 Glycine max DNA G24 7 482 Glycine max DNA G24 7 483 Glycine max DNA G24 7 484 Glycine max DNA G24 7 485 Glycine max DNA G24 7 486 Oryza sativa DNA G24 7 487 Zea mays DNA G24 7 488 Oryza sativa PRT G24 7 489 Oryza sativa PRT G24 7 490 Oryza sativa PRT G24 7 491 Glycine max DNA G28 9 492 Glycine max DNA G28 9 493 Glycine max DNA G28 9 494 Glycine max DNA G28 9 495 Glycine max DNA G28 9 496 Glycine max DNA G28 9 497 Glycine max DNA G28 9 498 Glycine max DNA G28 9 499 Oryza sativa DNA G28 9 500 Zea mays DNA G28 9 501 Oryza sativa PRT G28 9 502 Oryza sativa PRT G28 9 503 Mesembryanthemum PRT G28 9 crystallinum 504 Glycine max DNA G47, G2133 11, 407 505 Oryza sativa PRT G47, G2133 11, 407 506 Glycine max DNA G157, G859, 15, 165, 349, G1842, G1843 351 507 Glycine max DNA G175, G877 19, 173 508 Oryza sativa DNA G175, G877 19, 173 509 Zea mays DNA G175, G877 19, 173 510 Zea mays DNA G175, G877 19, 173 511 Zea mays DNA G175, G877 19, 173 512 Oryza sativa PRT G175, G877 19, 173 513 Oryza sativa PRT G175, G877 19, 173 514 Oryza sativa PRT G175, G877 19, 173 515 Nicotiana tabacum PRT G175, G877 19, 173 516 Glycine max DNA G180 21 517 Glycine max DNA G180 21 518 Oryza sativa DNA G180 21 519 Zea mays DNA G180 21 520 Solanum tuberosum DNA G180 21 521 Oryza sativa PRT G180 21 522 Capsella rubella PRT G183 23 523 Glycine max DNA G188 25 524 Zea mays DNA G188 25 525 Oryza sativa PRT G188 25 526 Oryza sativa PRT G188 25 527 Glycine max DNA G189 27 528 Nicotiana tabacum PRT G189 27 529 Glycine max DNA G192 29 530 Oryza sativa PRT G192 29 531 Glycine max DNA G196 31 532 Zea mays DNA G196 31 533 Zea mays DNA G196 31 534 Oryza sativa PRT G196 31 535 Oryza sativa PRT G196 31 536 Oryza sativa PRT G196 31 537 Oryza sativa PRT G196 31 538 Glycine max DNA G211 33 539 Oryza sativa DNA G211 33 540 Oryza sativa PRT G211 33 541 Glycine max DNA G214, G680 35, 145 542 Glycine max DNA G214, G680 35, 145 543 Glycine max DNA G214, G680 35, 145 544 Glycine max DNA G214, G680 35, 145 545 Oryza sativa DNA G214, G680 35, 145 546 Oryza sativa DNA G214, G680 35, 145 547 Zea mays DNA G214, G680 35, 145 548 Zea mays DNA G214, G680 35, 145 549 Zea mays DNA G214, G680 35, 145 550 Glycine max DNA G226, G682 37, 147 551 Glycine max DNA G226 37 552 Glycine max DNA G226, G682 37, 147 553 Glycine max DNA G226, G682 37, 147 554 Glycine max DNA G226, G682 37, 147 555 Oryza sativa DNA G226, G682 37, 147 556 Zea mays DNA G226, G682 37, 147 557 Zea mays DNA G226, G682 37, 147 558 Oryza sativa PRT G226, G682 37, 147 559 Oryza sativa PRT G226, G682 37, 147 560 Glycine max DNA G241 39 561 Glycine max DNA G241 39 562 Glycine max DNA G241 39 563 Oryza sativa DNA G241 39 564 Zea mays DNA G241 39 565 Zea mays DNA G241 39 566 Zea mays DNA G241 39 567 Zea mays DNA G241 39 568 Zea mays DNA G241 39 569 Nicotiana tabacum PRT G241 39 570 Glycine max DNA G254 43 571 Glycine max DNA G256 45 572 Glycine max DNA G256 45 573 Glycine max DNA G256 45 574 Glycine max DNA G256 45 575 Glycine max DNA G256 45 576 Glycine max DNA G256 45 577 Glycine max DNA G256 45 578 Oryza sativa DNA G256 45 579 Zea mays DNA G256 45 580 Zea mays DNA G256 45 581 Zea mays DNA G256 45 582 Zea mays DNA G256 45 583 Zea mays DNA G256 45 584 Zea mays DNA G256 45 585 G3500 Lycopersicon DNA G256 45 esculentum 586 G3501 Lycopersicon DNA G256 45 esculentum 587 G3385 Oryza sativa PRT G256 45 588 G3386 Oryza sativa PRT G256 45 589 Oryza sativa PRT G256 45 590 G3384 Oryza sativa PRT G256 45 591 Oryza sativa PRT G256 45 592 G3502 Oryza sativa japonica PRT G256 45 593 G3500 Lycopersicon PRT G256 45 esculentum 594 G3501 Lycopersicon PRT G256 45 esculentum 595 Oryza sativa DNA G278 47 596 Zea mays DNA G278 47 597 Oryza sativa PRT G278 47 598 Glycine max DNA G312 53 599 Zea mays DNA G312 53 600 Euphorbia esula DNA G312 53 601 Glycine max DNA G325 55 602 Glycine max DNA G343 57 603 Glycine max DNA G343 57 604 Glycine max DNA G343 57 605 Oryza sativa DNA G343 57 606 Oryza sativa DNA G343 57 607 Oryza sativa PRT G343 57 608 Oryza sativa PRT G343 57 609 Oryza sativa PRT G343 57 610 Glycine max DNA G353, G354 59, 61 611 Glycine max DNA G353, G354 59, 61 612 Glycine max DNA G353, G354 59, 61 613 Oryza sativa DNA G353, G354 59, 61 614 Zea mays DNA G353, G354 59, 61 615 Zea mays DNA G353, G354 59, 61 616 Zea mays DNA G353, G354 59, 61 617 Zea mays DNA G353, G354 59, 61 618 Zea mays DNA G353, G354 59, 61 619 Zea mays DNA G353, G354 59, 61 620 Zea mays DNA G353, G354 59, 61 621 Oryza sativa PRT G353, G354 59, 61 622 Oryza sativa PRT G353, G354 59, 61 623 Oryza sativa PRT G353, G354 59, 61 624 Oryza sativa PRT G353, G354 59, 61 625 Oryza sativa PRT G353, G354 59, 61 626 Oryza sativa PRT G353, G354 59, 61 627 Glycine max DNA G361, G362 63, 65 628 Glycine max DNA G361, G362 63, 65 629 Glycine max DNA G361 63 630 Glycine max DNA G361, G362 63, 65 631 Glycine max DNA G361, G362 63, 65 632 Oryza sativa DNA G361, G362 63, 65 633 Zea mays DNA G361, G362 63, 65 634 Zea mays DNA G361, G362 63, 65 635 Oryza sativa PRT G361, G362 63, 65 636 Oryza sativa PRT G361, G362 63, 65 637 Oryza sativa PRT G361, G362 63, 65 638 Oryza sativa PRT G361, G362 63, 65 639 Oryza sativa PRT G361, G362 63, 65 640 Glycine max DNA G390, G391, 69, 71, 77 G438 641 Glycine max DNA G390, G391, 69, 71, 77 G438 642 Glycine max DNA G390, G391, 69, 71, 77 G438 643 Glycine max DNA G390, G391, 69, 71, 77 G438 644 Glycine max DNA G390, G391, 69, 71, 77 G438 645 Glycine max DNA G390, G391, 69, 71, 77 G438 646 Glycine max DNA G390, G391, 69, 71, 77 G438 647 Glycine max DNA G390, G391 69, 71 648 Glycine max DNA G390, G391, 69, 71, 77 G438 649 Glycine max DNA G390, G391, 69, 71, 77 G438 650 Oryza sativa DNA G390 69 651 Oryza sativa DNA G390, G438 69, 77 652 Zea mays DNA G390, G391, 69, 71, 77 G438 653 Zea mays DNA G390, G391, 69, 71, 77 G438 654 Zea mays DNA G390, G391, 69, 71, 77 G438 655 Zea mays DNA G390, G391 69, 71 656 Zea mays DNA G390, G391, 69, 71, 77 G438 657 Zea mays DNA G390, G391, 69, 71, 77 G438 658 Zea mays DNA G390, G391, 69, 71, 77 G438 659 Zea mays DNA G390, G391, 69, 71, 77 G438 660 Zea mays DNA G390, G391, 69, 71, 77 G438 661 Zea mays DNA G390, G391, 69, 71, 77 G438 662 Zea mays DNA G390, G391, 69, 71, 77 G438 663 Lycopersicon DNA G390, G391, 69, 71, 77 esculentum G438 664 Oryza sativa DNA G391, G438 71, 77 665 Oryza sativa PRT G390, G391, 69, 71, 77 G438 666 Oryza sativa PRT G390, G391, 69, 71, 77 G438 667 Oryza sativa PRT G390, G391, 69, 71, 77 G438 668 Oryza sativa PRT G390, G391, 69, 71, 77 G438 669 Physcomitrella PRT G391 71 patens 670 Glycine max DNA G409 73 671 Glycine max DNA G409 73 672 Glycine max DNA G409 73 673 Glycine max DNA G409 73 674 Glycine max DNA G409 73 675 Glycine max DNA G409 73 676 Glycine max DNA G409 73 677 Glycine max DNA G409 73 678 Oryza sativa DNA G409 73 679 Oryza sativa DNA G409 73 680 Oryza sativa DNA G409 73 681 Zea mays DNA G409 73 682 Zea mays DNA G409 73 683 Zea mays DNA G409 73 684 Zea mays DNA G409 73 685 Zea mays DNA G409 73 686 Zea mays DNA G409 73 687 Zea mays DNA G409 73 688 Glycine max DNA G427 75 689 Glycine max DNA G427 75 690 Glycine max DNA G427 75 691 Glycine max DNA G427 75 692 Glycine max DNA G427 75 693 Glycine max DNA G427 75 694 Glycine max DNA G427 75 695 Glycine max DNA G427 75 696 Glycine max DNA G427 75 697 Glycine max DNA G427 75 698 Oryza sativa DNA G427 75 699 Zea mays DNA G427 75 700 Zea mays DNA G427 75 701 Zea mays DNA G427 75 702 Zea mays DNA G427 75 703 Zea mays DNA G427 75 704 Zea mays DNA G427 75 705 Zea mays DNA G427 75 706 Zea mays DNA G427 75 707 Zea mays DNA G427 75 708 Oryza sativa PRT G427 75 709 Oryza sativa PRT G427 75 710 Oryza sativa PRT G427 75 711 Malus x domestica PRT G427 75 712 Nicotiana tabacum PRT G427 75 713 Lycopersicon PRT G427 75 esculentum 714 Glycine max DNA G438 77 715 Oryza sativa DNA G438 77 716 Oryza sativa DNA G438 77 717 Oryza sativa DNA G438 77 718 Oryza sativa DNA G438 77 719 Zea mays DNA G438 77 720 Physcomitrella PRT G438 77 patens 721 Oryza sativa PRT G438 77 722 Glycine max DNA G450 79 723 Glycine max DNA G450 79 724 Glycine max DNA G450 79 725 Glycine max DNA G450 79 726 Glycine max DNA G450 79 727 Glycine max DNA G450 79 728 Glycine max DNA G450 79 729 Glycine max DNA G450 79 730 Glycine max DNA G450 79 731 Oryza sativa DNA G450 79 732 Oryza sativa DNA G450 79 733 Zea mays DNA G450 79 734 Zea mays DNA G450 79 735 Zea mays DNA G450 79 736 Oryza sativa PRT G450 79 737 Oryza sativa PRT G450 79 738 Oryza sativa PRT G450 79 739 Oryza sativa PRT G450 79 740 Oryza sativa DNA G464 81 741 Zea mays DNA G464 81 742 Oryza sativa PRT G464 81 743 Glycine max DNA G470 83 744 Oryza sativa DNA G470 83 745 Oryza sativa DNA G470 83 746 Glycine max DNA G481, G482 87, 89 747 Glycine max DNA G481, G482 87, 89 748 Glycine max DNA G481, G482 87, 89 749 Glycine max DNA G481, G482 87, 89 750 Glycine max DNA G481, G482 87, 89 751 Glycine max DNA G481, G482 87, 89 752 Glycine max DNA G481, G482 87, 89 753 Glycine max DNA G481, G482 87, 89 754 Glycine max DNA G481 87 755 Glycine max DNA G481 87 756 Oryza sativa DNA G481 87 757 Oryza sativa DNA G481, G482 87, 89 758 Zea mays DNA G481 87 759 Zea mays DNA G481, G482 87, 89 760 Zea mays DNA G481, G482 87, 89 761 Zea mays DNA G481, G482 87, 89 762 Zea mays DNA G481, G482 87, 89 763 Zea mays DNA G481, G482 87, 89 764 Zea mays DNA G481, G482 87, 89 765 Zea mays DNA G481, G482 87, 89 766 Zea mays DNA G481, G482 87, 89 767 Zea mays DNA G481, G482 87, 89 768 Gossypium arboreum DNA G481, G482 87, 89 769 Glycine max DNA G481, G482 87, 89 770 Gossypium hirsutum DNA G481, G482 87, 89 771 Lycopersicon DNA G481, G482 87, 89 esculentum 772 Lycopersicon DNA G481, G482 87, 89 esculentum 773 Medicago truncatula DNA G481, G482 87, 89 774 Lycopersicon DNA G481, G482 87, 89 esculentum 775 Solanum tuberosum DNA G481, G482 87, 89 776 Triticum aestivum DNA G481, G482 87, 89 777 Hordeum vulgare DNA G481, G482 87, 89 778 Triticum DNA G481, G482 87, 89 monococcum 779 Glycine max DNA G482 89 780 Oryza sativa PRT G481, G482 87, 89 781 Oryza sativa PRT G481, G482 87, 89 782 Oryza sativa PRT G481, G482 87, 89 783 Oryza sativa PRT G481, G482 87, 89 784 Oryza sativa PRT G481, G482 87, 89 785 Zea mays PRT G481, G482 87, 89 786 Zea mays PRT G481, G482 87, 89 787 Oryza sativa PRT G481, G482 87, 89 788 Oryza sativa PRT G481, G482 87, 89 789 Oryza sativa PRT G481, G482 87, 89 790 Oryza sativa PRT G481, G482 87, 89 791 Oryza sativa PRT G481, G482 87, 89 792 Oryza sativa PRT G481, G482 87, 89 793 Oryza sativa PRT G481, G482 87, 89 794 Oryza sativa PRT G481, G482 87, 89 795 Oryza sativa PRT G481, G482 87, 89 796 Oryza sativa PRT G481, G482 87, 89 797 Glycine max PRT G481, G482 87, 89 798 Glycine max PRT G481, G482 87, 89 799 Glycine max PRT G481, G482 87, 89 800 Glycine max PRT G481, G482 87, 89 801 Glycine max PRT G481, G482 87, 89 802 Glycine max PRT G481, G482 87, 89 803 Glycine max PRT G481, G482 87, 89 804 Zea mays PRT G481, G482 87, 89 805 Zea mays PRT G481, G482 87, 89 806 Zea mays PRT G481, G482 87, 89 807 Zea mays PRT G481, G482 87, 89 808 Glycine max DNA G484 91 809 Glycine max DNA G484 91 810 Glycine max DNA G484 91 811 Glycine max DNA G484 91 812 Glycine max DNA G484 91 813 Glycine max DNA G484 91 814 Glycine max DNA G484 91 815 Glycine max DNA G484 91 816 Glycine max DNA G484 91 817 Glycine max DNA G484 91 818 Oryza sativa DNA G484 91 819 Zea mays DNA G484 91 820 Zea mays DNA G484 91 821 Zea mays DNA G484 91 822 Zea mays DNA G484 91 823 Zea mays DNA G484 91 824 Oryza sativa PRT G484 91 825 Glycine max DNA G489 93 826 Glycine max DNA G489 93 827 Glycine max DNA G489 93 828 Glycine max DNA G489 93 829 Glycine max DNA G489 93 830 Glycine max DNA G489 93 831 Glycine max DNA G489 93 832 Oryza sativa DNA G489 93 833 Oryza sativa DNA G489 93 834 Zea mays DNA G489 93 835 Oryza sativa PRT G489 93 836 Oryza sativa PRT G489 93 837 Oryza sativa PRT G489 93 838 Glycine max DNA G504 97 839 Glycine max DNA G504 97 840 Glycine max DNA G504 97 841 Glycine max DNA G504 97 842 Glycine max DNA G504 97 843 Glycine max DNA G504 97 844 Glycine max DNA G504 97 845 Oryza sativa DNA G504 97 846 Oryza sativa DNA G504 97 847 Zea mays DNA G504 97 848 Zea mays DNA G504 97 849 Zea mays DNA G504 97 850 Zea mays DNA G504 97 851 Oryza sativa PRT G504 97 852 Oryza sativa PRT G504 97 853 Oryza sativa PRT G504 97 854 Oryza sativa PRT G504 97 855 Lycopersicon DNA G509 99 esculentum 856 Glycine max DNA G509 99 857 Glycine max DNA G509 99 858 Glycine max DNA G509 99 859 Oryza sativa DNA G509 99 860 Oryza sativa DNA G509 99 861 Zea mays DNA G509 99 862 Zea mays DNA G509 99 863 Zea mays DNA G509 99 864 Zea mays DNA G509 99 865 Oryza sativa PRT G509 99 866 Oryza sativa PRT G509 99 867 Oryza sativa PRT G509 99 868 Glycine max DNA G519 101 869 Glycine max DNA G519 101 870 Glycine max DNA G519 101 871 Glycine max DNA G519 101 872 Glycine max DNA G519 101 873 Glycine max DNA G519 101 874 Glycine max DNA G519 101 875 Glycine max DNA G519 101 876 Glycine max DNA G519 101 877 Oryza sativa DNA G519 101 878 Oryza sativa DNA G519 101 879 Oryza sativa DNA G519 101 880 Zea mays DNA G519 101 881 Zea mays DNA G519 101 882 Zea mays DNA G519 101 883 Zea mays DNA G519 101 884 Zea mays DNA G519 101 885 Zea mays DNA G519 101 886 Zea mays DNA G519 101 887 Zea mays DNA G519 101 888 Zea mays DNA G519 101 889 Zea mays DNA G519 101 890 Oryza sativa PRT G519 101 891 Oryza sativa PRT G519 101 892 Glycine max DNA G545 103 893 Glycine max DNA G545 103 894 Glycine max DNA G545 103 895 Glycine max DNA G545 103 896 Glycine max DNA G545 103 897 Glycine max DNA G545 103 898 Glycine max DNA G545 103 899 Oryza sativa DNA G545 103 900 Zea mays DNA G545 103 901 Zea mays DNA G545 103 902 Zea mays DNA G545 103 903 Oryza sativa PRT G545 103 904 Oryza sativa PRT G545 103 905 Oryza sativa PRT G545 103 906 Oryza sativa PRT G545 103 907 Datisca glomerata PRT G545 103 908 Oryza sativa DNA G546 105 909 Zea mays DNA G561 107 910 Sinapis alba PRT G561 107 911 Raphanus sativus PRT G561 107 912 Brassica napus PRT G561 107 913 Brassica napus PRT G561 107 914 Glycine max DNA G562 109 915 Glycine max DNA G562 109 916 Glycine max DNA G562 109 917 Glycine max DNA G562 109 918 Glycine max DNA G562 109 919 Zea mays DNA G562 109 920 Zea mays DNA G562 109 921 Zea mays DNA G562 109 922 Oryza sativa PRT G562 109 923 Oryza sativa PRT G562 109 924 Glycine max DNA G567 111 925 Oryza sativa DNA G567 111 926 Oryza sativa PRT G567 111 927 Glycine max DNA G568 113 928 Glycine max DNA G568 113 929 Oryza sativa DNA G568 113 930 Oryza sativa DNA G568 113 931 Oryza sativa DNA G568 113 932 Zea mays DNA G568 113 933 Oryza sativa PRT G568 113 934 Populus balsamifera PRT G568 113 subsp. trichocarpa x Populus deltoides 935 Glycine max DNA G584 115 936 Glycine max DNA G584 115 937 Glycine max DNA G584 115 938 Glycine max DNA G584 115 939 Glycine max DNA G584 115 940 Zea mays DNA G584 115 941 Zea mays DNA G584 115 942 Zea mays DNA G584 115 943 Oryza sativa PRT G584 115 944 Glycine max DNA G585 117 945 Glycine max DNA G585 117 946 Glycine max DNA G585 117 947 Glycine max DNA G585 117 948 Oryza sativa DNA G585 117 949 Zea mays DNA G585 117 950 Zea mays DNA G585 117 951 Zea mays DNA G585 117 952 Zea mays DNA G585 117 953 Oryza sativa PRT G585 117 954 Oryza sativa PRT G585 117 955 Oryza sativa PRT G585 117 956 Oryza sativa PRT G585 117 957 Oryza sativa PRT G585 117 958 Oryza sativa PRT G585 117 959 Gossypium hirsutum PRT G585 117 960 Antirrhinum majus PRT G585 117 961 Glycine max DNA G590 119 962 Glycine max DNA G590 119 963 Glycine max DNA G590 119 964 Oryza sativa DNA G590 119 965 Zea mays DNA G590 119 966 Oryza sativa PRT G590 119 967 Oryza sativa PRT G590 119 968 Oryza sativa DNA G597 123 969 Oryza sativa DNA G597 123 970 Oryza sativa DNA G597 123 971 Zea mays DNA G597 123 972 Zea mays DNA G597 123 973 Zea mays DNA G597 123 974 Zea mays DNA G597 123 975 Zea mays DNA G597 123 976 Zea mays DNA G597 123 977 Zea mays DNA G597 123 978 Zea mays DNA G597 123 979 Zea mays DNA G597 123 980 Zea mays DNA G597 123 981 Oryza sativa DNA G634 127 982 Oryza sativa DNA G634 127 983 Oryza sativa DNA G634 127 984 Zea mays DNA G634 127 985 Zea mays DNA G634 127 986 Zea mays DNA G634 127 987 Oryza sativa PRT G634 127 988 Oryza sativa PRT G634 127 989 Glycine max DNA G635 129 990 Glycine max DNA G635 129 991 Oryza sativa DNA G635 129 992 Oryza sativa DNA G635 129 993 Zea mays DNA G635 129 994 Oryza sativa PRT G635 129 995 Glycine max DNA G636 131 996 Glycine max DNA G636 131 997 Glycine max DNA G636 131 998 Glycine max DNA G636 131 999 Glycine max DNA G636 131 1000 Glycine max DNA G636 131 1001 Glycine max DNA G636 131 1002 Glycine max DNA G636 131 1003 Oryza sativa DNA G636 131 1004 Oryza sativa DNA G636 131 1005 Oryza sativa DNA G636 131 1006 Oryza sativa DNA G636 131 1007 Zea mays DNA G636 131 1008 Zea mays DNA G636 131 1009 Zea mays DNA G636 131 1010 Zea mays DNA G636 131 1011 Pisum sativum PRT G636 131 1012 Glycine max DNA G638 133 1013 Glycine max DNA G638 133 1014 Glycine max DNA G638 133 1015 Glycine max DNA G638 133 1016 Medicago truncatula DNA G638 133 1017 Glycine max DNA G652 135 1018 Glycine max DNA G652 135 1019 Glycine max DNA G652 135 1020 Glycine max DNA G652 135 1021 Glycine max DNA G652 135 1022 Glycine max DNA G652 135 1023 Glycine max DNA G652 135 1024 Glycine max DNA G652 135 1025 Oryza sativa DNA G652 135 1026 Oryza sativa DNA G652 135 1027 Oryza sativa DNA G652 135 1028 Zea mays DNA G652 135 1029 Zea mays DNA G652 135 1030 Zea mays DNA G652 135 1031 Zea mays DNA G652 135 1032 Zea mays DNA G652 135 1033 Zea mays DNA G652 135 1034 Zea mays DNA G652 135 1035 Oryza sativa PRT G652 135 1036 Oryza sativa PRT G652 135 1037 Oryza sativa PRT G652 135 1038 Oryza sativa PRT G652 135 1039 Oryza sativa PRT G652 135 1040 Oryza sativa PRT G652 135 1041 Oryza sativa PRT G652 135 1042 Oryza sativa PRT G652 135 1043 Glycine max DNA G663 137 1044 Glycine max DNA G664 139 1045 Glycine max DNA G664 139 1046 Glycine max DNA G664 139 1047 Glycine max DNA G664 139 1048 Glycine max DNA G664 139 1049 Glycine max DNA G664 139 1050 Glycine max DNA G664 139 1051 Oryza sativa DNA G664 139 1052 Oryza sativa DNA G664 139 1053 Oryza sativa DNA G664 139 1054 Oryza sativa DNA G664 139 1055 Zea mays DNA G664 139 1056 Zea mays DNA G664 139 1057 Zea mays DNA G664 139 1058 Zea mays DNA G664 139 1059 Zea mays DNA G664 139 1060 Zea mays DNA G664 139 1061 Zea mays DNA G664 139 1062 Zea mays DNA G664 139 1063 G3509 Lycopersicon DNA G664 139 esculentum 1064 G3506 Oryza sativa PRT G664 139 1065 G3504 Oryza sativa PRT G664 139 1066 Oryza sativa PRT G664 139 1067 Oryza sativa PRT G664 139 1068 G3503 Oryza sativa indica PRT G664 139 1069 G3505 Oryza sativa japonica PRT G664 139 1070 G3507 Oryza sativa japonica PRT G664 139 1071 G3508 Oryza sativa japonica PRT G664 139 1072 G3509 Lycopersicon PRT G664 139 esculentum 1073 Hordeum vulgare PRT G664 139 subsp. vulgare 1074 Oryza sativa DNA G680 145 1075 Zea mays DNA G680 145 1076 Glycine max DNA G682 147 1077 Hordeum vulgare DNA G682 147 subsp. vulgare 1078 Populus tremula x DNA G682 147 Populus tremuloides 1079 Triticum aestivum DNA G682 147 1080 Gossypium arboreum DNA G682 147 1081 Oryza sativa PRT G682 147 1082 Oryza sativa PRT G682 147 1083 Glycine max PRT G682 147 1084 Glycine max PRT G682 147 1085 Glycine max PRT G682 147 1086 Glycine max PRT G682 147 1087 Glycine max PRT G682 147 1088 Glycine max PRT G682 147 1089 Zea mays PRT G682 147 1090 Zea mays PRT G682 147 1091 Glycine max DNA G715, G1646 149, 313 1092 Glycine max DNA G715, G1646 149, 313 1093 Glycine max DNA G715, G1646 149, 313 1094 Oryza sativa DNA G715, G1646 149, 313 1095 Oryza sativa DNA G715, G1646 149, 313 1096 Zea mays DNA G715, G1646 149, 313 1097 Zea mays DNA G715, G1646 149, 313 1098 Zea mays DNA G715, G1646 149, 313 1099 Zea mays DNA G715, G1646 149, 313 1100 Zea mays DNA G715, G1646 149, 313 1101 Zea mays DNA G715, G1646 149, 313 1102 Zea mays DNA G715, G1646 149, 313 1103 Zea mays DNA G715, G1646 149, 313 1104 Zea mays DNA G715, G1646 149, 313 1105 Oryza sativa PRT G715, G1646 149, 313 1106 Oryza sativa PRT G715, G1646 149, 313 1107 Oryza sativa PRT G715, G1646 149, 313 1108 Oryza sativa PRT G715, G1646 149, 313 1109 Oryza sativa PRT G715, G1646 149, 313 1110 Oryza sativa PRT G715, G1646 149, 313 1111 Glycine max DNA G720 151 1112 Glycine max DNA G720 151 1113 Glycine max DNA G720 151 1114 Glycine max DNA G720 151 1115 Medicago truncatula DNA G720 151 1116 Lycopersicon DNA G720 151 esculentum 1117 Lycopersicon DNA G720 151 esculentum 1118 Lycopersicon DNA G720 151 esculentum 1119 Solanum tuberosum DNA G720 151 1120 Glycine max DNA G736 153 1121 Glycine max DNA G736 153 1122 Oryza sativa PRT G736 153 1123 Glycine max DNA G748 155 1124 Glycine max DNA G748 155 1125 Glycine max DNA G748 155 1126 Oryza sativa DNA G748 155 1127 Oryza sativa DNA G748 155 1128 Zea mays DNA G748 155 1129 Oryza sativa PRT G748 155 1130 Oryza sativa PRT G748 155 1131 Oryza sativa PRT G748 155 1132 Oryza sativa PRT G748 155 1133 Cucurbita maxima PRT G748 155 1134 Glycine max DNA G789, G1494 159, 291 1135 Glycine max DNA G789, G1494 159, 291 1136 Oryza sativa DNA G789 159 1137 Oryza sativa DNA G789, G1494 159, 291 1138 Zea mays DNA G789, G1494 159, 291 1139 Oryza sativa PRT G789, G1494 159, 291 1140 Oryza sativa PRT G789, G1494 159, 291 1141 Oryza sativa PRT G789, G1494 159, 291 1142 Glycine max DNA G801 161 1143 Glycine max DNA G801 161 1144 Zea mays DNA G801 161 1145 Glycine max DNA G849 163 1146 Glycine max DNA G849 163 1147 Glycine max DNA G849 163 1148 Glycine max DNA G849 163 1149 Glycine max DNA G849 163 1150 Glycine max DNA G849 163 1151 Zea mays DNA G849 163 1152 Zea mays DNA G849 163 1153 Zea mays DNA G849 163 1154 Glycine max DNA G864 167 1155 Glycine max DNA G864 167 1156 Zea mays DNA G864 167 1157 Oryza sativa PRT G864 167 1158 Oryza sativa PRT G864 167 1159 Glycine max DNA G867, G1930 169, 369 1160 Glycine max DNA G867, G1930 169, 369 1161 Glycine max DNA G867, G1930 169, 369 1162 Glycine max DNA G867, G1930 169, 369 1163 Glycine max DNA G867, G1930 169, 369 1164 Glycine max DNA G867 169 1165 Oryza sativa DNA G867 169 1166 Oryza sativa DNA G867, G1930 169, 369 1167 Zea mays DNA G867, G1930 169, 369 1168 Zea mays DNA G867, G1930 169, 369 1169 Zea mays DNA G867, G1930 169, 369 1170 Zea mays DNA G867, G1930 169, 369 1171 Glycine max DNA G867, G1930 169, 369 1172 Mesembryanthemum DNA G867, G1930 169, 369 crystallinum 1173 Lycopersicon DNA G867, G1930 169, 369 esculentum 1174 Solanum tuberosum DNA G867, G1930 169, 369 1175 Hordeum vulgare DNA G867, G1930 169, 369 1176 Oryza sativa PRT G867, G1930 169, 369 1177 Oryza sativa PRT G867, G1930 169, 369 1178 Oryza sativa PRT G867, G1930 169, 369 1179 Oryza sativa PRT G867, G1930 169, 369 1180 Oryza sativa PRT G867, G1930 169, 369 1181 Oryza sativa PRT G867, G1930 169, 369 1182 Glycine max PRT G867, G1930 169, 369 1183 Glycine max PRT G867, G1930 169, 369 1184 Glycine max PRT G867, G1930 169, 369 1185 Zea mays PRT G867, G1930 169, 369 1186 Zea mays PRT G867, G1930 169, 369 1187 Glycine max DNA G869 171 1188 Glycine max DNA G869 171 1189 Oryza sativa DNA G869 171 1190 Zea mays DNA G869 171 1191 Oryza sativa PRT G869 171 1192 Oryza sativa DNA G877 173 1193 Glycine max DNA G881 175 1194 Oryza sativa DNA G881 175 1195 Oryza sativa DNA G881 175 1196 Zea mays DNA G881 175 1197 Zea mays DNA G881 175 1198 Zea mays DNA G881 175 1199 Zea mays DNA G881 175 1200 Oryza sativa PRT G881 175 1201 Oryza sativa PRT G892 177 1202 Mentha x piperita DNA G896 179 1203 Glycine max DNA G910 181 1204 Glycine max DNA G912 185 1205 Glycine max DNA G912 185 1206 Glycine max DNA G912 185 1207 Glycine max DNA G912 185 1208 Glycine max DNA G912 185 1209 Glycine max DNA G912 185 1210 Glycine max DNA G912 185 1211 Oryza sativa DNA G912 185 1212 Oryza sativa DNA G912, G913 185, 187 1213 Zea mays DNA G912 185 1214 Zea mays DNA G912 185 1215 Zea mays DNA G912, G913 185, 187 1216 Zea mays DNA G912 185 1217 Zea mays DNA G912 185 1218 Brassica napus DNA G912, G913 185, 187 1219 Solanum tuberosum DNA G912 185 1220 Descurainia sophia DNA G912 185 1221 Oryza sativa PRT G912 185 1222 Oryza sativa PRT G912, G913 185, 187 1223 Oryza sativa PRT G912, G913 185, 187 1224 Oryza sativa PRT G912 185 1225 Brassica napus PRT G912 185 1226 Nicotiana tabacum PRT G912 185 1227 Oryza sativa PRT G912 185 1228 Oryza sativa PRT G912 185 1229 Oryza sativa PRT G912 185 1230 Oryza sativa PRT G912 185 1231 Oryza sativa PRT G912 185 1232 Oryza sativa PRT G912 185 1233 Oryza sativa PRT G912 185 1234 Oryza sativa PRT G912 185 1235 Oryza sativa PRT G912 185 1236 Oryza sativa PRT G912 185 1237 Glycine max PRT G912 185 1238 Glycine max PRT G912 185 1239 Glycine max PRT G912 185 1240 Glycine max PRT G912 185 1241 Glycine max PRT G912 185 1242 Glycine max PRT G912 185 1243 Glycine max PRT G912 185 1244 Zea mays PRT G912 185 1245 Zea mays PRT G912 185 1246 Zea mays PRT G912 185 1247 Zea mays PRT G912 185 1248 Zea mays PRT G912 185 1249 Glycine max DNA G922 189 1250 Glycine max DNA G922 189 1251 Glycine max DNA G922 189 1252 Oryza sativa DNA G922 189 1253 Oryza sativa DNA G922 189 1254 Oryza sativa PRT G922 189 1255 Oryza sativa PRT G922 189 1256 Oryza sativa PRT G922 189 1257 Oryza sativa PRT G922 189 1258 Glycine max DNA G926 191 1259 Glycine max DNA G926 191 1260 Oryza sativa DNA G926 191 1261 Oryza sativa DNA G926 191 1262 Zea mays DNA G926 191 1263 Brassica napus PRT G926 191 1264 Glycine max DNA G961 193 1265 Glycine max DNA G961 193 1266 Oryza sativa DNA G961 193 1267 Zea mays DNA G961 193 1268 Zea mays DNA G961 193 1269 Zea mays DNA G961 193 1270 Oryza sativa PRT G961 193 1271 Glycine max DNA G974 197 1272 Glycine max DNA G974 197 1273 Glycine max DNA G974 197 1274 Glycine max DNA G974 197 1275 Glycine max DNA G974 197 1276 Glycine max DNA G974 197 1277 Oryza sativa DNA G974 197 1278 Zea mays DNA G974 197 1279 Zea mays DNA G974 197 1280 Zea mays DNA G974 197 1281 Zea mays DNA G974 197 1282 Lycopersicon DNA G974 197 esculentum 1283 Glycine max DNA G974 197 1284 Solanum tuberosum DNA G974 197 1285 Poplar xylem DNA G974 197 1286 Medicago truncatula DNA G974 197 1287 Sorghum bicolor DNA G974 197 1288 Oryza sativa PRT G974 197 1289 Oryza sativa PRT G974 197 1290 Oryza sativa PRT G974 197 1291 Atriplex hortensis PRT G974 197 1292 Glycine max DNA G975, G2583 199, 449 1293 Glycine max DNA G975, G2583 199, 449 1294 Glycine max DNA G975, G2583 199, 449 1295 Glycine max DNA G975, G2583 199, 449 1296 Glycine max DNA G975, G2583 199, 449 1297 Oryza sativa DNA G975 199 1298 Oryza sativa DNA G975, G2583 199, 449 1299 Zea mays DNA G975, G2583 199, 449 1300 Zea mays DNA G975, G2583 199, 449 1301 Brassica rapa DNA G975, G2583 199, 449 1302 Oryza sativa PRT G975, G2583 199, 449 1303 Glycine max DNA G979 201 1304 Glycine max DNA G979 201 1305 Glycine max DNA G979 201 1306 Oryza sativa DNA G979 201 1307 Zea mays DNA G979 201 1308 Zea mays DNA G979 201 1309 Zea mays DNA G979 201 1310 Oryza sativa PRT G979 201 1311 Oryza sativa PRT G979 201 1312 Oryza sativa PRT G979 201 1313 Oryza sativa PRT G979 201 1314 Oryza sativa PRT G979 201 1315 Glycine max DNA G987 203 1316 Glycine max DNA G987 203 1317 Glycine max DNA G987 203 1318 Glycine max DNA G987 203 1319 Glycine max DNA G987 203 1320 Glycine max DNA G987 203 1321 Oryza sativa DNA G987 203 1322 Oryza sativa DNA G987 203 1323 Zea mays DNA G987 203 1324 Oryza sativa PRT G987 203 1325 Oryza sativa PRT G988 205 1326 Oryza sativa PRT G988 205 1327 Capsella rubella PRT G988 205 1328 Glycine max DNA G1040 207 1329 Glycine max DNA G1040 207 1330 Glycine max DNA G1040 207 1331 Glycine max DNA G1040 207 1332 Glycine max DNA G1040 207 1333 Zea mays DNA G1040 207 1334 Zea mays DNA G1040 207 1335 Zea mays DNA G1040 207 1336 Zea mays DNA G1040 207 1337 Zea mays DNA G1040 207 1338 Oryza sativa PRT G1040 207 1339 Oryza sativa PRT G1040 207 1340 Glycine max DNA G1047 209 1341 Zea mays DNA G1047 209 1342 Oryza sativa PRT G1047 209 1343 Oryza sativa PRT G1047 209 1344 Glycine max DNA G1051, G1052 211, 213 1345 Glycine max DNA G1051, G1052 211, 213 1346 Glycine max DNA G1051, G1052 211, 213 1347 Glycine max DNA G1051, G1052 211, 213 1348 Glycine max DNA G1051, G1052 211, 213 1349 Glycine max DNA G1051, G1052 211, 213 1350 Glycine max DNA G1051, G1052 211, 213 1351 Oryza sativa DNA G1051, G1052 211, 213 1352 Zea mays DNA G1051, G1052 211, 213 1353 Zea mays DNA G1051, G1052 211, 213 1354 Zea mays DNA G1051, G1052 211, 213 1355 Zea mays DNA G1051, G1052 211, 213 1356 Zea mays DNA G1051, G1052 211, 213 1357 Zea mays DNA G1051, G1052 211, 213 1358 Zea mays DNA G1051, G1052 211, 213 1359 Oryza sativa DNA G1052 213 1360 Zea mays DNA G1052 213 1361 Zea mays DNA G1052 213 1362 Oryza sativa PRT G1051, G1052 211, 213 1363 Oryza sativa PRT G1051, G1052 211, 213 1364 Oryza sativa PRT G1051, G1052 211, 213 1365 Glycine max DNA G1062 215 1366 Glycine max DNA G1062 215 1367 Glycine max DNA G1062 215 1368 Glycine max DNA G1062 215 1369 Oryza sativa DNA G1062 215 1370 Oryza sativa DNA G1062 215 1371 Zea mays DNA G1062 215 1372 Zea mays DNA G1062 215 1373 Zea mays DNA G1062 215 1374 Zea mays DNA G1062 215 1375 Zea mays DNA G1062 215 1376 Medicago truncatula DNA G1062 215 1377 Lycopersicon DNA G1062 215 esculentum 1378 Oryza sativa PRT G1062 215 1379 Glycine max DNA G1063, G2143 217, 413 1380 Glycine max DNA G1063, G2143 217, 413 1381 Glycine max DNA G1063, G2143 217, 413 1382 Glycine max DNA G1063, G2143 217, 413 1383 Glycine max DNA G1063, G2143 217, 413 1384 Lycopersicon DNA G1063, G2143 217, 413 esculentum 1385 Glycine max DNA G1064 219 1386 Glycine max DNA G1064 219 1387 Glycine max DNA G1064 219 1388 Zea mays DNA G1064 219 1389 Zea mays DNA G1064 219 1390 Lycopersicon DNA G1064 219 esculentum 1391 Oryza sativa PRT G1064 219 1392 Gossypium hirsutum PRT G1064 219 1393 Glycine max DNA G1069 221 1394 Glycine max DNA G1069 221 1395 Oryza sativa PRT G1069, G1073 221, 223 1396 Zea mays DNA G1069 221 1397 Lotus japonicus DNA G1069 221 1398 Lycopersicon DNA G1073 223 esculentum 1399 Oryza sativa PRT G1073 223 1400 Oryza sativa PRT G1073 223 1401 Oryza sativa PRT G1073 223 1402 Oryza sativa PRT G1073 223 1403 Oryza sativa PRT G1073 223 1404 Oryza sativa PRT G1073 223 1405 Oryza sativa PRT G1073 223 1406 Oryza sativa PRT G1073 223 1407 Oryza sativa PRT G1073 223 1408 Oryza sativa PRT G1073 223 1409 Oryza sativa PRT G1073 223 1410 Oryza sativa PRT G1073 223 1411 Glycine max PRT G1073 223 1412 Glycine max PRT G1073 223 1413 Glycine max PRT G1073 223 1414 Glycine max PRT G1073 223 1415 Glycine max PRT G1073 223 1416 Glycine max PRT G1073 223 1417 Glycine max PRT G1073 223 1418 Zea mays PRT G1073 223 1419 Glycine max DNA G1075 225 1420 Glycine max DNA G1075 225 1421 Glycine max DNA G1075 225 1422 Glycine max DNA G1075 225 1423 Glycine max DNA G1075 225 1424 Oryza sativa DNA G1075 225 1425 Oryza sativa DNA G1075 225 1426 Oryza sativa DNA G1075 225 1427 Oryza sativa DNA G1089 229 1428 Zea mays DNA G1089 229 1429 Zea mays DNA G1089 229 1430 Zea mays DNA G1089 229 1431 Zea mays DNA G1089 229 1432 Zea mays DNA G1089 229 1433 Oryza sativa PRT G1089 229 1434 Glycine max DNA G1134, G2555 231, 445 1435 Glycine max DNA G1134, G2555 231, 445 1436 Oryza sativa DNA G1134, G2555 231, 445 1437 Glycine max DNA G1140 233 1438 Glycine max DNA G1140 233 1439 Glycine max DNA G1140 233 1440 Glycine max DNA G1140 233 1441 Glycine max DNA G1140 233 1442 Glycine max DNA G1140 233 1443 Oryza sativa DNA G1140 233 1444 Zea mays DNA G1140 233 1445 Zea mays DNA G1140 233 1446 Zea mays DNA G1140 233 1447 Zea mays DNA G1140 233 1448 Zea mays DNA G1140 233 1449 Zea mays DNA G1140 233 1450 Zea mays DNA G1140 233 1451 Zea mays DNA G1140 233 1452 Zea mays DNA G1140 233 1453 Oryza sativa PRT G1140 233 1454 Ipomoea batatas PRT G1140 233 1455 Zea mays DNA G1146 237 1456 Zea mays DNA G1146 237 1457 Oryza sativa PRT G1146 237 1458 Oryza sativa PRT G1146 237 1459 Oryza sativa PRT G1146 237 1460 Glycine max DNA G1196 239 1461 Glycine max DNA G1196 239 1462 Glycine max DNA G1196 239 1463 Oryza sativa DNA G1196 239 1464 Zea mays DNA G1196 239 1465 Zea mays DNA G1196 239 1466 Oryza sativa PRT G1196 239 1467 Oryza sativa PRT G1196 239 1468 Glycine max DNA G1198 241 1469 Glycine max DNA G1198 241 1470 Glycine max DNA G1198 241 1471 Glycine max DNA G1198 241 1472 Glycine max DNA G1198 241 1473 Glycine max DNA G1198 241 1474 Glycine max DNA G1198 241 1475 Glycine max DNA G1198 241 1476 Oryza sativa DNA G1198 241 1477 Oryza sativa DNA G1198 241 1478 Oryza sativa DNA G1198 241 1479 Oryza sativa DNA G1198 241 1480 Oryza sativa DNA G1198 241 1481 Zea mays DNA G1198 241 1482 Zea mays DNA G1198 241 1483 Zea mays DNA G1198 241 1484 Zea mays DNA G1198 241 1485 Zea mays DNA G1198 241 1486 Zea mays DNA G1198 241 1487 Zea mays DNA G1198 241 1488 Zea mays DNA G1198 241 1489 Zea mays DNA G1198 241 1490 Zea mays DNA G1198 241 1491 Nicotiana tabacum DNA G1198 241 1492 Oryza sativa PRT G1198 241 1493 Oryza sativa PRT G1198 241 1494 Oryza sativa PRT G1198 241 1495 Oryza sativa PRT G1198 241 1496 Oryza sativa PRT G1198 241 1497 Oryza sativa PRT G1198 241 1498 Oryza sativa PRT G1198 241 1499 Zea mays DNA G1225 243 1500 Oryza sativa PRT G1225 243 1501 Oryza sativa PRT G1226 245 1502 Glycine max DNA G1229 247 1503 Oryza sativa PRT G1229 247 1504 Oryza sativa PRT G1229 247 1505 Glycine max DNA G1255 249 1506 Glycine max DNA G1255 249 1507 Glycine max DNA G1255 249 1508 Glycine max DNA G1255 249 1509 Glycine max DNA G1255 249 1510 Glycine max DNA G1255 249 1511 Glycine max DNA G1255 249 1512 Oryza sativa DNA G1255 249 1513 Oryza sativa DNA G1255 249 1514 Oryza sativa DNA G1255 249 1515 Oryza sativa DNA G1255 249 1516 Zea mays DNA G1255 249 1517 Zea mays DNA G1255 249 1518 Zea mays DNA G1255 249 1519 Zea mays DNA G1255 249 1520 Zea mays DNA G1255 249 1521 Zea mays DNA G1255 249 1522 Oryza sativa PRT G1255 249 1523 Glycine max DNA G1266 251 1524 Glycine max DNA G1266 251 1525 Glycine max DNA G1266 251 1526 Glycine max DNA G1266 251 1527 Oryza sativa DNA G1266 251 1528 Nicotiana tabacum PRT G1266 251 1529 Oryza sativa DNA G1275 253 1530 Zea mays DNA G1275 253 1531 Zea mays DNA G1275 253 1532 Zea mays DNA G1275 253 1533 Oryza sativa PRT G1275 253 1534 Oryza sativa PRT G1275 253 1535 Oryza sativa PRT G1275 253 1536 Glycine max DNA G1322 257 1537 Glycine max DNA G1322 257 1538 Glycine max DNA G1322 257 1539 Oryza sativa DNA G1322 257 1540 Oryza sativa PRT G1322 257 1541 Oryza sativa PRT G1322 257 1542 Zea mays DNA G1323 259 1543 Zea mays DNA G1323 259 1544 Glycine max DNA G1330 261 1545 Glycine max DNA G1330 261 1546 Glycine max DNA G1330 261 1547 Glycine max DNA G1330 261 1548 Glycine max DNA G1330 261 1549 Glycine max DNA G1330 261 1550 Glycine max DNA G1330 261 1551 Oryza sativa DNA G1330 261 1552 Oryza sativa DNA G1330 261 1553 Oryza sativa DNA G1330 261 1554 Oryza sativa DNA G1330 261 1555 Zea mays DNA G1330 261 1556 Zea mays DNA G1330 261 1557 Zea mays DNA G1330 261 1558 Zea mays DNA G1330 261 1559 Zea mays DNA G1330 261 1560 Zea mays DNA G1330 261 1561 Zea mays DNA G1330 261 1562 Lycopersicon DNA G1330 261 esculentum 1563 Oryza sativa PRT G1330 261 1564 Oryza sativa PRT G1330 261 1565 Oryza sativa PRT G1330 261 1566 Oryza sativa PRT G1330 261 1567 Glycine max DNA G1331 263 1568 Glycine max DNA G1331 263 1569 Oryza sativa DNA G1331 263 1570 Zea mays DNA G1331 263 1571 Zea mays DNA G1331 263 1572 Oryza sativa PRT G1331 263 1573 Glycine max DNA G1363 267 1574 Oryza sativa DNA G1363 267 1575 Oryza sativa DNA G1363 267 1576 Oryza sativa DNA G1363 267 1577 Oryza sativa DNA G1363 267 1578 Zea mays DNA G1363 267 1579 Zea mays DNA G1363 267 1580 Zea mays DNA G1363 267 1581 Zea mays DNA G1363 267 1582 Zea mays DNA G1363 267 1583 Oryza sativa PRT G1363 267 1584 Oryza sativa PRT G1363 267 1585 Oryza sativa PRT G1363 267 1586 Oryza sativa PRT G1363 267 1587 Glycine max DNA G1411, G2509 269, 439 1588 Glycine max DNA G1411, G2509 269, 439 1589 Glycine max DNA G1411, G2509 269, 439 1590 Glycine max DNA G1411, G2509 269, 439 1591 Zea mays DNA G1411, G2509 269, 439 1592 Glycine max DNA G1417 271 1593 Oryza sativa PRT G1417 271 1594 Oryza sativa PRT G1417 271 1595 Glycine max DNA G1419 273 1596 Glycine max DNA G1449 275 1597 Glycine max DNA G1449 275 1598 Oryza sativa DNA G1449 275 1599 Oryza sativa DNA G1449 275 1600 Zea mays DNA G1449 275 1601 Zea mays DNA G1449 275 1602 Zea mays DNA G1449 275 1603 Zea mays DNA G1449 275 1604 Glycine max DNA G1451 277 1605 Glycine max DNA G1451 277 1606 Oryza sativa DNA G1451 277 1607 Oryza sativa DNA G1451 277 1608 Oryza sativa DNA G1451 277 1609 Zea mays DNA G1451 277 1610 Zea mays DNA G1451 277 1611 Zea mays DNA G1451 277 1612 Zea mays DNA G1451 277 1613 Medicago truncatula DNA G1451 277 1614 Solanum tuberosum DNA G1451 277 1615 Zea mays DNA G1451 277 1616 Sorghum DNA G1451 277 propinquum 1617 Glycine max DNA G1451 277 1618 Sorghum bicolor DNA G1451 277 1619 Hordeum vulgare DNA G1451 277 1620 Lycopersicon DNA G1451 277 esculentum 1621 Oryza sativa PRT G1451 277 1622 Oryza sativa PRT G1451 277 1623 Oryza sativa PRT G1451 277 1624 Oryza sativa PRT G1451 277 1625 Glycine max DNA G1452 279 1626 Glycine max DNA G1478 285 1627 Glycine max DNA G1478 285 1628 Glycine max DNA G1478 285 1629 Zea mays DNA G1478 285 1630 Glycine max DNA G1482 287 1631 Glycine max DNA G1482 287 1632 Glycine max DNA G1482 287 1633 Glycine max DNA G1482 287 1634 Glycine max DNA G1482 287 1635 Oryza sativa DNA G1482 287 1636 Oryza sativa DNA G1482 287 1637 Oryza sativa DNA G1482 287 1638 Oryza sativa DNA G1482 287 1639 Zea mays DNA G1482 287 1640 Zea mays DNA G1482 287 1641 Zea mays DNA G1482 287 1642 Zea mays DNA G1482 287 1643 Zea mays DNA G1482 287 1644 Zea mays DNA G1482 287 1645 Oryza sativa PRT G1482 287 1646 Oryza sativa PRT G1482 287 1647 Glycine max DNA G1488 289 1648 Glycine max DNA G1488 289 1649 Glycine max DNA G1488 289 1650 Oryza sativa DNA G1488 289 1651 Oryza sativa DNA G1488 289 1652 Zea mays DNA G1488 289 1653 Zea mays DNA G1488 289 1654 Zea mays DNA G1488 289 1655 Oryza sativa PRT G1488 289 1656 Oryza sativa PRT G1488 289 1657 Oryza sativa PRT G1488 289 1658 Oryza sativa PRT G1499 295 1659 Brassica rapa subsp. DNA G1499 295 pekinensis 1660 Glycine max DNA G1519 297 1661 Oryza sativa DNA G1519 297 1662 Zea mays DNA G1519 297 1663 Zea mays DNA G1519 297 1664 Lycopersicon DNA G1519 297 esculentum 1665 Glycine max DNA G1526 2199 1666 Oryza sativa DNA G1526 299 1667 Oryza sativa DNA G1526 299 1668 Zea mays DNA G1526 299 1669 Glycine max DNA G1540 301 1670 Oryza sativa PRT G1540 301 1671 Glycine max DNA G1543 303 1672 Oryza sativa DNA G1543 303 1673 Zea mays DNA G1543 303 1674 Oryza sativa PRT G1543 303 1675 Zea mays DNA G1637 307 1676 Zea mays DNA G1637 307 1677 Zea mays DNA G1637 307 1678 Glycine max DNA G1640 309 1679 Glycine max DNA G1640 309 1680 Glycine max DNA G1640 309 1681 Oryza sativa PRT G1640 309 1682 Zea mays DNA G1645 311 1683 Zea mays DNA G1645 311 1684 Zea mays DNA G1645 311 1685 Lycopersicon DNA G1645 311 esculentum 1686 Medicago truncatula DNA G1645 311 1687 Oryza sativa PRT G1645 311 1688 Oryza sativa DNA G1646 313 1689 Oryza sativa DNA G1646 313 1690 Glycine max DNA G1652 315 1691 Glycine max DNA G1652 315 1692 Glycine max DNA G1652 315 1693 Glycine max DNA G1652 315 1694 Glycine max DNA G1652 315 1695 Glycine max DNA G1652 315 1696 Glycine max DNA G1652 315 1697 Glycine max DNA G1652 315 1698 Oryza sativa DNA G1652 315 1699 Zea mays DNA G1652 315 1700 Zea mays DNA G1652 315 1701 Oryza sativa PRT G1652 315 1702 Oryza sativa PRT G1652 315 1703 Oryza sativa PRT G1652 315 1704 Oryza sativa PRT G1652 315 1705 Oryza sativa PRT G1652 315 1706 Glycine max DNA G1672 317 1707 Oryza sativa DNA G1672 317 1708 Zea mays DNA G1672 317 1709 Zea mays DNA G1672 317 1710 Oryza sativa PRT G1672 317 1711 Oryza sativa PRT G1672 317 1712 Oryza sativa PRT G1672 317 1713 Oryza sativa PRT G1672 317 1714 Glycine max DNA G1750 323 1715 Glycine max DNA G1750 323 1716 Glycine max DNA G1750 323 1717 Glycine max DNA G1750 323 1718 Oryza sativa DNA G1750 323 1719 Zea mays DNA G1750 323 1720 Zea mays DNA G1750 323 1721 Glycine max DNA G1756 325 1722 Medicago truncatula DNA G1765 327 1723 Glycine max DNA G1777 329 1724 Oryza sativa DNA G1777 329 1725 Zea mays DNA G1777 329 1726 Zea mays DNA G1777 329 1727 Oryza sativa PRT G1777 329 1728 Glycine max DNA G1792 331 1729 Glycine max DNA G1792 331 1730 Glycine max DNA G1792 331 1731 Glycine max DNA G1792 331 1732 Glycine max DNA G1792 331 1733 Zea mays DNA G1792 331 1734 Lycopersicon DNA G1792 331 esculentum 1735 G3380 Oryza sativa PRT G1792 331 1736 G3381 Oryza sativa indica PRT G1792 331 1737 G3383 Oryza sativa japonica PRT G1792 331 1738 Glycine max DNA G1793 333 1739 Oryza sativa DNA G1793 333 1740 Zea mays DNA G1793 333 1741 Zea mays DNA G1793 333 1742 Zea mays DNA G1793 333 1743 Oryza sativa PRT G1793 333 1744 Glycine max DNA G1794 335 1745 Glycine max DNA G1794 335 1746 Glycine max DNA G1794 335 1747 Glycine max DNA G1794 335 1748 Glycine max DNA G1794 335 1749 Glycine max DNA G1794 335 1750 Glycine max DNA G1794 335 1751 Zea mays DNA G1794 335 1752 Zea mays DNA G1794 335 1753 Zea mays DNA G1794 335 1754 Oryza sativa PRT G1794 335 1755 Oryza sativa PRT G1794 335 1756 Oryza sativa PRT G1794 335 1757 Glycine max DNA G1804 337 1758 Glycine max DNA G1804 337 1759 Glycine max DNA G1804 337 1760 Oryza sativa DNA G1804 337 1761 Oryza sativa PRT G1804 337 1762 Helianthus annuus PRT G1804 337 1763 Glycine max DNA G1838 345 1764 Glycine max DNA G1838 345 1765 Oryza sativa PRT G1838 345 1766 Glycine max DNA G1841 347 1767 Glycine max DNA G1841 347 1768 Oryza sativa DNA G1841 347 1769 Oryza sativa PRT G1841 347 1770 Solanum tuberosum DNA G1852 353 1771 Gossypium arboreum DNA G1852 353 1772 Medicago truncatula DNA G1852 353 1773 Glycine max DNA G1852 353 1774 Lycopersicon DNA G1852 353 esculentum 1775 Pinus taeda DNA G1852 353 1776 Lotus japonicus DNA G1852 353 1777 Gossypium hirsutum DNA G1852 353 1778 Solanum tuberosum DNA G1863 355 1779 Medicago truncatula DNA G1863 355 1780 Lycopersicon DNA G1863 355 esculentum 1781 Oryza sativa PRT G1863 355 1782 Glycine max DNA G1880 357 1783 Glycine max DNA G1880 357 1784 Medicago truncatula DNA G1880 357 1785 Oryza sativa PRT G1880 357 1786 Glycine max DNA G1902 361 1787 Glycine max DNA G1902 361 1788 Glycine max DNA G1902 361 1789 Zea mays DNA G1902 361 1790 Oryza sativa PRT G1902 361 1791 Glycine max DNA G1927 367 1792 Oryza sativa DNA G1927 367 1793 Zea mays DNA G1927 367 1794 Lycopersicon DNA G1927 367 esculentum 1795 Oryza sativa DNA G1930 369 1796 Glycine max DNA G1944 373 1797 Glycine max DNA G1944 373 1798 Zea mays DNA G1944 373 1799 Glycine max DNA G1944 373 1800 Glycine max DNA G1944 373 1801 Glycine max DNA G1946 375 1802 Glycine max DNA G1946 375 1803 Zea mays DNA G1946 375 1804 Zea mays DNA G1946 375 1805 Oryza sativa PRT G1946 375 1806 Glycine max DNA G1948 379 1807 Glycine max DNA G1948 379 1808 Oryza sativa DNA G1948 379 1809 Oryza sativa DNA G1948 379 1810 Zea mays DNA G1948 379 1811 Zea mays DNA G1948 379 1812 Zea mays DNA G1948 379 1813 Oryza sativa PRT G1948 379 1814 Glycine max DNA G1950 381 1815 Glycine max DNA G1950 381 1816 Glycine max DNA G1950 381 1817 Glycine max DNA G1950 381 1818 Glycine max DNA G1950 381 1819 Glycine max DNA G1950 381 1820 Oryza sativa DNA G1950 381 1821 Oryza sativa DNA G1950 381 1822 Oryza sativa DNA G1950 381 1823 Oryza sativa DNA G1950 381 1824 Oryza sativa DNA G1950 381 1825 Oryza sativa DNA G1950 381 1826 Oryza sativa DNA G1950 381 1827 Oryza sativa DNA G1950 381 1828 Oryza sativa DNA G1950 381 1829 Zea mays DNA G1950 381 1830 Zea mays DNA G1950 381 1831 Zea mays DNA G1950 381 1832 Zea mays DNA G1950 381 1833 Zea mays DNA G1950 381 1834 Zea mays DNA G1950 381 1835 Zea mays DNA G1950 381 1836 Zea mays DNA G1950 381 1837 Zea mays DNA G1950 381 1838 Oryza sativa PRT G1950 381 1839 Oryza sativa PRT G1950 381 1840 Oryza sativa PRT G1950 381 1841 Oryza sativa PRT G1950 381 1842 Oryza sativa PRT G1950 381 1843 Oryza sativa PRT G1950 381 1844 Oryza sativa PRT G1950 381 1845 Oryza sativa PRT G1950 381 1846 Oryza sativa PRT G1950 381 1847 Glycine max DNA G1958 383 1848 Glycine max DNA G1958 383 1849 Glycine max DNA G1958 383 1850 Glycine max DNA G1958 383 1851 Glycine max DNA G1958 383 1852 Oryza sativa DNA G1958 383 1853 Oryza sativa DNA G1958 383 1854 Zea mays DNA G1958 383 1855 Zea mays DNA G1958 383 1856 Zea mays DNA G1958 383 1857 Nicotiana tabacum PRT G1958 383 1858 Glycine max DNA G2007 385 1859 Glycine max DNA G2007 385 1860 Zea mays DNA G2007 385 1861 Zea mays DNA G2007 385 1862 Zea mays DNA G2007 385 1863 Oryza sativa PRT G2007 385 1864 Glycine max DNA G2010, G2347 387, 431 1865 Oryza sativa DNA G2010, G2347 387, 431 1866 Zea mays DNA G2010 387 1867 Zea mays DNA G2010, G2347 387, 431 1868 Glycine max DNA G2059 391 1869 Glycine max DNA G2085 393 1870 Glycine max DNA G2085 393 1871 Glycine max DNA G2085 393 1872 Glycine max DNA G2085 393 1873 Zea mays DNA G2085 393 1874 Oryza sativa PRT G2085 393 1875 Oryza sativa PRT G2105 395 1876 Glycine max DNA G2110 397 1877 Oryza sativa DNA G2114 399 1878 Oryza sativa DNA G2114 399 1879 Zea mays DNA G2114 399 1880 Zea mays DNA G2114 399 1881 Oryza sativa DNA G2117 401 1882 Medicago truncatula DNA G2130 405 1883 Oryza sativa PRT G2130 405 1884 Oryza sativa PRT G2130 405 1885 Glycine max DNA G2140 411 1886 Glycine max DNA G2140 411 1887 Glycine max DNA G2140 411 1888 Glycine max DNA G2140 411 1889 Glycine max DNA G2140 411 1890 Glycine max DNA G2140 411 1891 Oryza sativa DNA G2140 411 1892 Oryza sativa DNA G2140 411 1893 Oryza sativa DNA G2140 411 1894 Oryza sativa DNA G2140 411 1895 Zea mays DNA G2140 411 1896 Lycopersicon DNA G2140 411 esculentum 1897 Oryza sativa PRT G2140 411 1898 Oryza sativa PRT G2140 411 1899 Oryza sativa PRT G2140 411 1900 Oryza sativa PRT G2140 411 1901 Oryza sativa PRT G2140 411 1902 Glycine max DNA G2143 413 1903 Glycine max DNA G2143 413 1904 Glycine max DNA G2144 415 1905 Glycine max DNA G2144 415 1906 Zea mays DNA G2144 415 1907 Zea mays DNA G2144 415 1908 Medicago truncatula DNA G2155 419 1909 Medicago truncatula DNA G2155 419 1910 Glycine max DNA G2155 419 1911 Oryza sativa PRT G2192 421 1912 Oryza sativa PRT G2295 423 1913 Glycine max DNA G2340 425 1914 Glycine max DNA G2343 427 1915 Glycine max DNA G2343 427 1916 Glycine max DNA G2343 427 1917 Lycopersicon PRT G2343 427 esculentum 1918 Oryza sativa PRT G2379 433 1919 Oryza sativa PRT G2379 433 1920 Oryza sativa PRT G2379 433 1921 Glycine max DNA G2505 437 1922 Zea mays DNA G2505 437 1923 Glycine max DNA G2520 443 1924 Glycine max DNA G2520 443 1925 Oryza sativa DNA G2520 443 1926 Zea mays DNA G2520 443 1927 Zea mays DNA G2520 443 1928 Zea mays DNA G2520 443 1929 Oryza sativa PRT G2520 443 1930 Oryza sativa PRT G2520 443 1931 Glycine max DNA G2557 447 1932 Glycine max DNA G2557 447 1933 Glycine max DNA G2557 447 1934 Zea mays DNA G2557 447 1935 Zea mays DNA G2557 447 1936 Glycine max DNA G2557 447 1937 Oryza sativa PRT G2557 447 1938 Oryza sativa PRT G2557 447 1939 Oryza sativa PRT G2557 447 1940 Glycine max DNA G2719 453 1941 Zea mays DNA G2719 453 1942 Oryza sativa PRT G2719 453 1943 Oryza sativa PRT G2719 453 1944 Glycine max DNA G2789 455 1945 Medicago truncatula DNA G2789 455 1946 Glycine max DNA G2830 457

Table 8 lists a summary of homologous sequences identified using BLAST (tblastx program). The first column shows the polynucleotide sequence identifier (SEQ ID NO), the second column shows the corresponding cDNA identifier (Gene ID), the third column shows the orthologous or homologous polynucleotide GenBank Accession Number (Test Sequence ID), the fourth column shows the calculated probability value that the sequence identity is due to chance (Smallest Sum Probability), the fifth column shows the plant species from which the test sequence was isolated (Test Sequence Species), and the sixth column shows the orthologous or homologous test sequence GenBank annotation (Test Sequence GenBank Annotation).

TABLE 8 Summary of representative sequences that are homologous to presently-disclosed transcription factors Smallest Polynucleotide Test Sum Test Sequence GenBank SEQ ID NO: GID Sequence ID Probability Test Sequence Species Annotation 1 G8 AF134116 2.00E−92 Hyacinthus orientalis APETALA2 protein homolog HAP2 (HAP2) 1 G8 AF132002 6.00E−86 Petunia x hybrida PHAP2B protein (Ap2B) mRNA, complete cds. 1 G8 AF332215 8.00E−84 Malus x domestica transcription factor AHAP2 (AHAP2) mRNA, 1 G8 CA783794 3.00E−83 Glycine max sat57d09.y1 Gm-c1056 Glycine max cDNA clone SOY 1 G8 AY069953 7.00E−82 Hordeum vulgare APETALA2-like protein (AP2L1) mRNA, complet 1 G8 AF253971 5.00E−81 Picea abies APETALA2-related transcription factor 2 (AP2L2) 1 G8 AF048900 2.00E−80 Zea mays indeterminate spikelet 1 (ids1) mRNA, complete cds 1 G8 AF325506 4.00E−80 Pisum sativum APETAL2-like protein mRNA, complete cds. 1 G8 BG321674 6.00E−79 Descurainia sophia Ds01_06a02_A Ds01_AAFC_ECORC_cold_stress 1 G8 BQ120583 3.00E−78 Solanum tuberosum EST606159 mixed potato tissues Solanum tu 1 G8 gi24059986 1.30E−91 Oryza sativa (japonica putative indetermi cultivar-group) 1 G8 gi5360996 8.70E−88 Hyacinthus orientalis APETALA2 protein homolog HAP2. 1 G8 gi5081555 4.50E−86 Petunia x hybrida PHAP2A protein. 1 G8 gi2944040 5.80E−84 Zea mays indeterminate spikelet 1. 1 G8 gi21717332 9.30E−82 Malus x domestica transcription factor AHAP2. 1 G8 gi11181612 7.50E−78 Picea abies APETALA2-related transcription factor 2. 1 G8 gi13173164 1.60E−77 Pisum sativum APETAL2-like protein. 1 G8 gi18476518 2.60E−70 Hordeum vulgare APETALA2-like protein. 1 G8 gi21069051 1.40E−34 Brassica napus AP2/EREBP transcription factor BABY BOOM1. 1 G8 gi21304225 8.60E−33 Oryza sativa aintegumenta-like protein. 3 G19 BG321358 1.00E−101 Descurainia sophia Ds01_07d03_R Ds01_AAFC_ECORC_cold_stress 3 G19 BH444831 1.00E−77 Brassica oleracea BOHPW42TR BOHP Brassica oleracea genomic 3 G19 BM412184 2.00E−43 Lycopersicon EST586511 tomato breaker esculentum fruit Lyco 3 G19 BU837697 3.00E−43 Populus tremula x T104G02 Populus apica Populus tremuloides 3 G19 CA784650 6.00E−43 Glycine max sat87a10.y1 Gm-c1062 Glycine max cDNA clone SOY 3 G19 BU819833 3.00E−41 Populus tremula UA48BPB07 Populus tremula cambium cDNA libr 3 G19 BU870388 4.00E−41 Populus balsamifera Q011H05 Populus flow subsp. trichocarpa 3 G19 CA797119 1.00E−38 Theobroma cacao Cac_BL_4204 Cac_BL (Bean and Leaf from Amel 3 G19 BI436183 2.00E−38 Solanum tuberosum EST538944 cSTE Solanum tuberosum cDNA clo 3 G19 BQ989448 2.00E−36 Lactuca sativa QGF17L05.yg.ab1 QG_EFGHJ lettuce serriola La 3 G19 gi10798644 5.70E−36 Nicotiana tabacum AP2 domain-containing transcription fac 3 G19 gi6176534 2.40E−35 Oryza sativa EREBP-like protein. 3 G19 gi1688233 7.50E−34 Solanum tuberosum DNA binding protein homolog. 3 G19 gi22074046 1.50E−33 Lycopersicon transcription factor JERF1. esculentum 3 G19 gi18496063 4.90E−33 Fagus sylvatica ethylene responsive element binding prote 3 G19 gi20805105 2.10E−32 Oryza sativa (japonica contains ESTs AU06 cultivar-group) 3 G19 gi24940524 2.30E−31 Triticum aestivum ethylene response element binding prote 3 G19 gi18266198 2.30E−31 Narcissus AP-2 domain containing pseudonarcissus protein. 3 G19 gi3264767 1.30E−30 Prunus armeniaca AP2 domain containing protein. 3 G19 gi24817250 4.00E−28 Cicer arietinum transcription factor EREBP- like protein. 5 G22 AB016264 9.00E−48 Nicotiana sylvestris nserf2 gene for ethylene- responsive el 5 G22 TOBBY4A 1.00E−47 Nicotiana tabacum mRNA for ERF1, complete cds. 5 G22 AP004533 4.00E−47 Lotus japonicus genomic DNA, chromosome 3, clone: LjT14G02, 5 G22 LEU89255 6.00E−47 Lycopersicon DNA-binding protein Pti4 esculentum mRNA, comp 5 G22 BQ517082 6.00E−46 Solanum tuberosum EST624497 Generation of a set of potato c 5 G22 BE449392 1.00E−45 Lycopersicon hirsutum EST356151 L. hirsutum trichome, Corne 5 G22 AF245119 5.00E−45 Mesembryanthemum AP2-related transcription crystallinum fac 5 G22 BQ165291 7.00E−45 Medicago truncatula EST611160 KVKC Medicago truncatula cDNA 5 G22 AW618245 8.00E−38 Lycopersicon pennellii EST314295 L. pennellii trichome, Cor 5 G22 BG444654 2.00E−36 Gossypium arboreum GA_Ea0025B11f Gossypium arboreum 7-10 d 5 G22 gi1208495 6.10E−48 Nicotiana tabacum ERF1. 5 G22 gi3342211 3.30E−47 Lycopersicon Pti4. esculentum 5 G22 gi8809571 8.90E−47 Nicotiana sylvestris ethylene-responsive element binding 5 G22 gi17385636 2.70E−36 Matricaria chamomilla ethylene-responsive element binding 5 G22 gi8980313 2.50E−33 Catharanthus roseus AP2-domain DNA-binding protein. 5 G22 gi7528276 8.60E−33 Mesembryanthemum AP2-related transcription f crystallinum 5 G22 gi21304712 3.10E−28 Glycine max ethylene-responsive element binding protein 1 5 G22 gi14140141 1.50E−26 Oryza sativa putative AP2-related transcription factor. 5 G22 gi15623863 1.30E−22 Oryza sativa (japonica contains EST~hypot cultivar-group) 5 G22 gi4099914 3.10E−21 Stylosanthes hamata ethylene-responsive element binding p 7 G24 BZ026790 7.00E−71 Brassica oleracea oeh27a09.b1 B. oleracea002 Brassica olerac 7 G24 BM985484 4.00E−52 Thellungiella halophila 10_C12_T Ath Thellungiella halophil 7 G24 BQ405872 3.00E−45 Gossypium arboreum GA_Ed0088A03f Gossypium arboreum 7-10 d 7 G24 BG543187 3.00E−44 Brassica rapa subsp. E0677 Chinese cabbage pekinensis etiol 7 G24 AW981184 7.00E−42 Medicago truncatula EST392378 DSIL Medicago truncatula cDNA 7 G24 BQ704289 9.00E−41 Brassica napus Bn01_04f19_A 7 G24 BG321374 9.00E−40 Descurainia sophia Ds01_06d08_R Ds01_AAFC_ECORC_cold_stress 7 G24 OSIG00036 4.00E−37 Oryza sativa chromosome 4 clone H0721B11, *** SEQUENCING I 7 G24 AAAA01024762 4.00E−37 Oryza sativa (indica ( ) scaffold024762 cultivar-group) 7 G24 BQ586795 6.00E−37 Beta vulgaris E012390-024-012-J13-SP6 MPIZ-ADIS-024-leaf Be 7 G24 gi5091503 9.60E−34 Oryza sativa EST AU055776(S20048) corresponds to a region 7 G24 gi20161239 6.40E−21 Oryza sativa (japonica hypothetical prote cultivar-group) 7 G24 gi8980313 2.20E−20 Catharanthus roseus AP2-domain DNA-binding protein. 7 G24 gi4099921 2.80E−20 Stylosanthes hamata EREBP-3 homolog. 7 G24 gi10798644 5.70E−20 Nicotiana tabacum AP2 domain-containing transcription fac 7 G24 gi8571476 1.70E−18 Atriplex hortensis apetala2 domain-containing protein. 7 G24 gi8809573 2.10E−18 Nicotiana sylvestris ethylene-responsive element binding 7 G24 gi21908034 2.20E−18 Zea mays DRE binding factor 2. 7 G24 gi17352283 9.60E−18 Brassica napus CBF-like protein. 7 G24 gi3342211 4.70E−17 Lycopersicon Pti4. esculentum 9 G28 AF245119 2.00E−72 Mesembryanthemum AP2-related transcription crystallinum fac 9 G28 BQ165291 1.00E−68 Medicago truncatula EST611160 KVKC Medicago truncatula cDNA 9 G28 AB016264 1.00E−57 Nicotiana sylvestris nserf2 gene for ethylene- responsive el 9 G28 TOBBY4D 2.00E−57 Nicotiana tabacum Tobacco mRNA for EREBP-2, complete cds. 9 G28 BQ047502 2.00E−57 Solanum tuberosum EST596620 P. infestans- challenged potato 9 G28 LEU89255 2.00E−56 Lycopersicon DNA-binding protein Pti4 esculentum mRNA, comp 9 G28 BH454277 2.00E−54 Brassica oleracea BOGSI45TR BOGS Brassica oleracea genomic 9 G28 BE449392 1.00E−53 Lycopersicon hirsutum EST356151 L. hirsutum trichome, Corne 9 G28 AB035270 2.00E−50 Matricaria chamomilla McEREBP1 mRNA for ethylene-responsive 9 G28 AW233956 5.00E−50 Glycine max sf32e02.y1 Gm-c1028 Glycine max cDNA clone GENO 9 G28 gi7528276 6.10E−71 Mesembryanthemum AP2-related transcription f crystallinum 9 G28 gi8809571 3.30E−56 Nicotiana sylvestris ethylene-responsive element binding 9 G28 gi3342211 4.20E−56 Lycopersicon Pti4. esculentum 9 G28 gi1208498 8.70E−56 Nicotiana tabacum EREBP-2. 9 G28 gi14140141 4.20E−49 Oryza sativa putative AP2-related transcription factor. 9 G28 gi17385636 3.00E−46 Matricaria chamomilla ethylene-responsive element binding 9 G28 gi21304712 2.90E−31 Glycine max ethylene-responsive element binding protein 1 9 G28 gi15623863 5.60E−29 Oryza sativa (japonica contains EST~hypot cultivar-group) 9 G28 gi8980313 1.20E−26 Catharanthus roseus AP2-domain DNA-binding protein. 9 G28 gi4099921 3.10E−21 Stylosanthes hamata EREBP-3 homolog. 11 G47 BG543936 1.00E−60 Brassica rapa subsp. E1686 Chinese cabbage pekinensis etiol 11 G47 BH420519 3.00E−43 Brassica oleracea BOGUH88TF BOGU Brassica oleracea genomic 11 G47 AU292603 3.00E−30 Zinnia elegans AU292603 zinnia cultured mesophyll cell equa 11 G47 BE320193 1.00E−24 Medicago truncatula NF024B04RT1F1029 Developing root Medica 11 G47 AAAA01000718 1.00E−22 Oryza sativa (indica ( ) scaffold000718 cultivar-group) 11 G47 AP003379 2.00E−22 Oryza sativa chromosome 1 clone P0408G07, *** SEQUENCING IN 11 G47 AC124836 8.00E−21 Oryza sativa (japonica ( ) chromosome 5 clo cultivar-group) 11 G47 BZ403609 2.00E−20 Zea mays OGABN17TM ZM_0.7_1.5_KB Zea mays genomic clone ZMM 11 G47 BM112772 6.00E−17 Solanum tuberosum EST560308 potato roots Solanum tuberosum 11 G47 BQ698717 1.00E−16 Pinus taeda NXPV_148_C06_F NXPV (Nsf Xylem Planings wood Ve 11 G47 gi20161239 6.90E−24 Oryza sativa (japonica hypothetical prote cultivar-group) 11 G47 gi14140155 6.80E−17 Oryza sativa putative AP2 domain transcription factor. 11 G47 gi21908034 7.00E−15 Zea mays DRE binding factor 2. 11 G47 gi20303011 1.90E−14 Brassica napus CBF-like protein CBF5. 11 G47 gi8571476 3.00E−14 Atriplex hortensis apetala2 domain-containing protein. 11 G47 gi8980313 2.10E−13 Catharanthus roseus AP2-domain DNA-binding protein. 11 G47 gi19071243 4.40E−13 Hordeum vulgare CRT/DRE binding factor 1. 11 G47 gi18650662 5.60E−13 Lycopersicon ethylene response factor 1. esculentum 11 G47 gi17385636 1.20E−12 Matricaria chamomilla ethylene-responsive element binding 11 G47 gi1208498 1.50E−12 Nicotiana tabacum EREBP-2. 13 G156 AF335242 4.00E−45 Petunia x hybrida MADS-box transcription factor FBP24 (FBP2 13 G156 AMA307056 2.00E−41 Antirrhinum majus mRNA for putative MADS- domain transcript 13 G156 BF276751 1.00E−35 Gossypium arboreum GA_Eb0030I08f Gossypium arboreum 7-10 d 13 G156 AB071380 2.00E−35 Lilium regale LRGLOB mRNA for MADS-box transcription factor 13 G156 ZMA271208 2.00E−34 Zea mays mRNA for putative MADS- domain transcription facto 13 G156 AI899235 1.00E−33 Lycopersicon EST268678 tomato ovary, esculentum TAMU Lycope 13 G156 GGN132219 8.00E−33 Gnetum gnemon mRNA for putative MADS domain transcription 13 G156 BQ753907 2.00E−32 Hordeum vulgare subsp. EBca01_SQ002_D17_R vulgare carpel, p 13 G156 AF134114 1.00E−31 Hyacinthus orientalis PISTILLATA protein homolog HPI1 (HPI1 13 G156 AB094985 1.00E−30 Orchis italica OrcPI mRNA for PI/GLO- like protein, complete 13 G156 gi13384062 8.50E−42 Petunia x hybrida MADS-box transcription factor FBP24. 13 G156 gi19578307 2.00E−40 Antirrhinum majus putative MADS-domain transcription fact 13 G156 gi20513262 1.30E−36 Lilium regale MADS-box transcription factor. 13 G156 gi18076209 2.70E−36 Zea mays putative MADS-domain transcription factor. 13 G156 gi5019464 1.40E−34 Gnetum gnemon putative MADS domain transcription factor G 13 G156 gi3114586 7.10E−34 Eucalyptus grandis MADS box protein. 13 G156 gi4885036 9.00E−34 Hyacinthus orientalis PISTILLATA protein homolog HPI2. 13 G156 gi24421111 1.60E−31 Orchis italica PI/GLO-like protein. 13 G156 gi2961437 2.30E−31 Oryza sativa MADS box protein. 13 G156 gi16549070 3.40E−31 Magnolia praecocissima putative MADS-domain transcription 15 G157 AY036888 1.00E−63 Brassica napus MADS-box protein (FLC1) mRNA, complete cds. 15 G157 BG596731 1.00E−37 Solanum tuberosum EST495409 cSTS Solanum tuberosum cDNA clo 15 G157 BG544805 1.00E−37 Brassica rapa subsp. E2809 Chinese cabbage pekinensis etiol 15 G157 AW219962 4.00E−37 Lycopersicon EST302445 tomato root esculentum during/after 15 G157 BM436799 5.00E−36 Vitis vinifera VVA010B05_53181 An expressed sequence tag da 15 G157 BU875165 1.00E−31 Populus balsamifera V003A12 Populus flow subsp. trichocarpa 15 G157 BQ868455 2.00E−31 Lactuca sativa QGD14A13.yg.ab1 QG_ABCDI lettuce salinas Lac 15 G157 BI957545 1.00E−30 Hordeum vulgare HVSMEn0010B09f Hordeum vulgare rachis EST 1 15 G157 BJ213269 3.00E−30 Triticum aestivum BJ213269 Y. Ogihara unpublished cDNA libr 15 G157 BU887610 3.00E−30 Populus tremula x R064B01 Populus root Populus tremuloides 15 G157 gi17933450 4.90E−62 Brassica napus MADS-box protein. 15 G157 gi9367313 2.60E−31 Hordeum vulgare MADS-box protein 8. 15 G157 gi16874557 5.50E−31 Antirrhinum majus MADS-box transcription factor DEFH28. 15 G157 gi1483232 7.00E−31 Betula pendula MADS5 protein. 15 G157 gi4204234 1.40E−30 Lolium temulentum MADS-box protein 2. 15 G157 gi7592642 1.40E−30 Oryza sativa AP1-like MADS box protein. 15 G157 gi12002141 1.80E−30 Zea mays MADS box protein 3. 15 G157 gi21070923 1.80E−30 Oryza sativa (japonica AP1-like MADS-box cultivar-group) 15 G157 gi13384068 8.00E−30 Petunia x hybrida MADS-box transcription factor FBP29. 15 G157 gi6469345 1.30E−29 Brassica rapa subsp. DNA-binding protein. pekinensis 17 G162 BZ073323 6.00E−44 Brassica oleracea 1kf66e08.b1 B. oleracea002 Brassica olerac 17 G162 BQ403135 3.00E−33 Gossypium arboreum GA_Ed0054C07f Gossypium arboreum 7-10 d 17 G162 AC122160 2.00E−27 Medicago truncatula clone mth2-23d6, WORKING DRAFT SEQUENCE 17 G162 CRU91416 2.00E−18 Ceratopteris richardii CMADS2 mRNA, complete cds. 17 G162 AP005789 3.00E−18 Oryza sativa (japonica ( ) chromosome 9 clo cultivar-group) 17 G162 AAAA01007138 3.00E−18 Oryza sativa (indica ( ) scaffold007138 cultivar-group) 17 G162 AP003627 8.00E−18 Oryza sativa genomic DNA, chromosome 1, PAC clone: P0459B04, 17 G162 BZ415846 1.00E−16 Zea mays if60b04.g1 WGS-ZmaysF (DH5a methyl filtered) Zea m 17 G162 CA733624 3.00E−16 Triticum aestivum wlp1c.pk005.p15 wlp1c Triticum aestivum c 17 G162 AF035379 4.00E−16 Lolium temulentum MADS-box protein 2 (MADS2) mRNA, alternat 17 G162 gi3253149 1.30E−20 Ceratopteris richardii CMADS2. 17 G162 gi15290141 2.80E−20 Oryza sativa hypothetical protein. 17 G162 gi6580943 2.40E−19 Picea abies MADS-box transcription factor. 17 G162 gi5019431 4.90E−19 Gnetum gnemon putative MADS domain transcription factor G 17 G162 gi1206005 4.90E−19 Pinus radiata putative MADS-box family transcription fact 17 G162 gi1702951 4.90E−19 Pinus resinosa MADS-box family transcription factor. 17 G162 gi887392 8.00E−19 Brassica oleracea BOAP1. 17 G162 gi21396799 1.60E−18 Lycopodium annotinum MADS-box gene 4 protein. 17 G162 gi20219014 3.40E−18 Lycopersicon MADS-box transcription esculentum factor MAD 17 G162 gi7672991 3.60E−18 Canavalia lineata MADS-box transcription factor. 19 G175 AB063576 1.00E−108 Nicotiana tabacum NtWRKY-9 mRNA for WRKY DNA-binding protei 19 G175 LES303343 1.00E−103 Lycopersicon mRNA for hypothetical esculentum protein (ORF 19 G175 BZ005522 2.00E−74 Brassica oleracea oej73d10.b1 B. oleracea002 Brassica olerac 19 G175 IPBSPF1P 3.00E−71 Ipomoea batatas Sweet potato mRNA for SPF1 protein, complet 19 G175 AX192162 3.00E−68 Glycine max Sequence 9 from Patent WO0149840. 19 G175 AX192164 1.00E−66 Triticum aestivum Sequence 11 from Patent WO0149840. 19 G175 AF439274 5.00E−65 Retama raetam WRKY-like drought- induced protein (WRK) mRNA, 19 G175 OSJN00012 5.00E−64 Oryza sativa chromosome 4 clone OSJNBa0089K21, *** SEQUENC 19 G175 CUSSLDB 6.00E−63 Cucumis sativus SPF1-like DNA-binding protein mRNA, complet 19 G175 PCU48831 7.00E−63 Petroselinum crispum DNA-binding protein WRKY1 mRNA, comple 19 G175 gi13620227 8.20E−108 Lycopersicon hypothetical protein. esculentum 19 G175 gi14530687 2.00E−89 Nicotiana tabacum WRKY DNA-binding protein. 19 G175 gi1076685 2.10E−74 Ipomoea batatas SPF1 protein-sweet potato. 19 G175 gi18158619 1.10E−69 Retama raetam WRKY-like drought- induced protein. 19 G175 gi7484759 5.90E−68 Cucumis sativus SP8 binding protein homolog-cucumber. 19 G175 gi5917653 7.80E−64 Petroselinum crispum zinc-finger type transcription facto 19 G175 gi14587365 2.40E−63 Oryza sativa putative DNA-binding protein ABF1. 19 G175 gi4894965 9.90E−61 Avena sativa DNA-binding protein WRKY1. 19 G175 gi1159877 2.40E−60 Avena fatua DNA-binding protein. 19 G175 gi16588566 7.30E−52 Solanum dulcamara thermal hysteresis protein STHP-64. 21 G180 BU896559 7.00E−66 Populus tremula x X042D08 Populus wood Populus tremuloides 21 G180 CA800201 2.00E−58 Glycine max sat79d02.y1 Gm-c1062 Glycine max cDNA clone SOY 21 G180 BQ507128 8.00E−55 Solanum tuberosum EST614543 Generation of a set of potato c 21 G180 BJ322852 1.00E−39 Triticum aestivum BJ322852 Y. Ogihara unpublished cDNA libr 21 G180 BQ293390 8.00E−39 Zea mays 1091013C10.x2 1091- Immature ear with common ESTs 21 G180 BM370440 9.00E−30 Hordeum vulgare EBro08_SQ004_D21_R IGF Barley EBro08 librar 21 G180 AF140554 3.00E−28 Avena sativa DNA-binding protein WRKY1 (wrky1) mRNA, comple 21 G180 BI210061 1.00E−27 Lycopersicon EST528101 cTOS esculentum Lycopersicon esculen 21 G180 AFABF1 4.00E−27 Avena fatua A. fatua mRNA for DNA- binding protein (clone ABF 21 G180 BQ864325 2.00E−26 Lactuca sativa QGC26J22.yg.ab1 QG_ABCDI lettuce salinas Lac 21 G180 gi14140117 9.60E−50 Oryza sativa WRKY-like DNA-binding protein. 21 G180 gi24745606 1.10E−31 Solanum tuberosum WRKY-type DNA binding protein. 21 G180 gi4894965 1.90E−29 Avena sativa DNA-binding protein WRKY1. 21 G180 gi1159877 3.50E−29 Avena fatua DNA-binding protein. 21 G180 gi20161004 5.60E−29 Oryza sativa (japonica hypothetical prote cultivar-group) 21 G180 gi1431872 7.30E−29 Petroselinum crispum WRKY1. 21 G180 gi5360683 6.90E−28 Nicotiana tabacum NtWRKY1. 21 G180 gi13620227 3.50E−27 Lycopersicon hypothetical protein. esculentum 21 G180 gi3420906 5.30E−27 Pimpinella brachycarpa zinc finger protein; WRKY1. 21 G180 gi1076685 1.20E−26 Ipomoea batatas SPF1 protein-sweet potato. 23 G183 CRU303349 3.00E−54 Capsella rubella ORF1, ORF2, ORF3, ORF4, ORF5 and ORF6 (pa 23 G183 AB063576 5.00E−33 Nicotiana tabacum NtWRKY-9 mRNA for WRKY DNA-binding protei 23 G183 LES303343 3.00E−32 Lycopersicon mRNA for hypothetical esculentum protein (ORF 23 G183 IPBSPF1P 2.00E−29 Ipomoea batatas Sweet potato mRNA for SPF1 protein, complet 23 G183 BM408205 2.00E−29 Solanum tuberosum EST582532 potato roots Solanum tuberosum 23 G183 BI128063 5.00E−29 Populus tremula x G070P32Y Populus camb Populus tremuloides 23 G183 BU043758 1.00E−28 Prunus persica PP_LEa0017B09f Peach developing fruit mesoca 23 G183 AX192162 4.00E−28 Glycine max Sequence 9 from Patent WO0149840. 23 G183 BG442954 5.00E−28 Gossypium arboreum GA_Ea0018P14f Gossypium arboreum 7-10 d 23 G183 AF080595 2.00E−27 Pimpinella brachycarpa zinc finger protein (ZFP1) mRNA, com 23 G183 gi13620168 1.30E−86 Capsella rubella hypothetical protein. 23 G183 gi13620227 2.60E−52 Lycopersicon hypothetical protein. esculentum 23 G183 gi6174838 1.10E−37 Nicotiana tabacum transcription factor NtWRKY4. 23 G183 gi1076685 1.70E−35 Ipomoea batatas SPF1 protein-sweet potato. 23 G183 gi7484759 9.20E−29 Cucumis sativus SP8 binding protein homolog-cucumber. 23 G183 gi1159877 9.50E−29 Avena fatua DNA-binding protein. 23 G183 gi14587365 8.00E−28 Oryza sativa putative DNA-binding protein ABF1. 23 G183 gi3420906 1.10E−27 Pimpinella brachycarpa zinc finger protein; WRKY1. 23 G183 gi5917653 1.00E−26 Petroselinum crispum zinc-finger type transcription facto 23 G183 gi4894965 2.30E−26 Avena sativa DNA-binding protein WRKY1. 25 G188 AW596933 6.00E−43 Glycine max sj84f07.y1 Gm-c1034 Glycine max cDNA clone GENO 25 G188 BI923414 2.00E−40 Lycopersicon EST543319 tomato callus esculentum Lycopersico 25 G188 AV423663 3.00E−40 Lotus japonicus AV423663 Lotus japonicus young plants (two- 25 G188 BM112869 6.00E−39 Solanum tuberosum EST560405 potato roots Solanum tuberosum 25 G188 AP003951 6.00E−39 Oryza sativa chromosome 6 clone OJ1288_C01, *** SEQUENCING 25 G188 AP004683 9.00E−39 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 25 G188 AAAA01011017 9.00E−39 Oryza sativa (indica ( ) scaffold011017 cultivar-group) 25 G188 BU837263 6.00E−38 Populus tremula x T096G05 Populus apica Populus tremuloides 25 G188 AW447931 4.00E−34 Triticum aestivum BRY_1082 BRY Triticum aestivum cDNA clone 25 G188 BQ763996 2.00E−32 Hordeum vulgare subsp. EBro03_SQ006_A04_R vulgare root, 3 w 25 G188 gi12039364 4.00E−37 Oryza sativa putative DNA-binding protein. 25 G188 gi4322940 4.70E−21 Nicotiana tabacum DNA-binding protein 2. 25 G188 gi4894963 5.00E−20 Avena sativa DNA-binding protein WRKY3. 25 G188 gi1432056 7.80E−20 Petroselinum crispum WRKY3. 25 G188 gi11993901 3.10E−18 Dactylis glomerata somatic embryogenesis related protein. 25 G188 gi22830985 1.10E−17 Oryza sativa (japonica WRKY transcription cultivar-group) 25 G188 gi7484759 1.40E−16 Cucumis sativus SP8 binding protein homolog-cucumber. 25 G188 gi1159879 2.70E−15 Avena fatua DNA-binding protein. 25 G188 gi23305051 8.00E−15 Oryza sativa (indica WRKY transcription f cultivar-group) 25 G188 gi9187622 2.70E−14 Solanum tuberosum WRKY DNA binding protein. 27 G189 AB041520 2.00E−67 Nicotiana tabacum mRNA for WRKY transcription factor Nt-Sub 27 G189 PCU56834 2.00E−64 Petroselinum crispum DNA binding protein WRKY3 mRNA, comple 27 G189 AF140553 6.00E−55 Avena sativa DNA-binding protein WRKY3 (wrky3) mRNA, comple 27 G189 BI469529 1.00E−54 Glycine max sah61a11.y1 Gm-c1049 Glycine max cDNA clone GEN 27 G189 AY108689 5.00E−54 Zea mays PCO134907 mRNA sequence. 27 G189 AAAA01014145 7.00E−54 Oryza sativa (indica ( ) scaffold014145 cultivar-group) 27 G189 BI209749 2.00E−53 Lycopersicon EST527789 cTOS esculentum Lycopersicon esculen 27 G189 BU046845 4.00E−53 Prunus persica PP_LEa0027O15f Peach developing fruit mesoca 27 G189 AP004648 4.00E−51 Oryza sativa (japonica ( ) chromosome 8 clo cultivar-group) 27 G189 OSJN00198 6.00E−48 Oryza sativa chromosome 4 clone OSJNBb0015N08, *** SEQUENC 27 G189 gi4894963 1.00E−54 Avena sativa DNA-binding protein WRKY3. 27 G189 gi10798760 1.70E−50 Nicotiana tabacum WRKY transcription factor Nt-SubD48. 27 G189 gi1432056 1.60E−49 Petroselinum crispum WRKY3. 27 G189 gi11993901 5.80E−43 Dactylis glomerata somatic embryogenesis related protein. 27 G189 gi15289829 5.60E−25 Oryza sativa contains ESTs D24303(R1701), C26098(C11628) ~u 27 G189 gi1076685 1.60E−21 Ipomoea batatas SPF1 protein-sweet potato. 27 G189 gi1159877 6.50E−21 Avena fatua DNA-binding protein. 27 G189 gi18158619 5.10E−20 Retama raetam WRKY-like drought- induced protein. 27 G189 gi3420906 9.80E−20 Pimpinella brachycarpa zinc finger protein; WRKY1. 27 G189 gi23305051 4.50E−19 Oryza sativa (indica WRKY transcription f cultivar-group) 29 G192 BH471182 3.00E−62 Brassica oleracea BOHES67TF BOHE Brassica oleracea genomic 29 G192 BI923235 2.00E−49 Lycopersicon EST543139 tomato callus esculentum Lycopersico 29 G192 AW596933 3.00E−47 Glycine max sj84f07.y1 Gm-c1034 Glycine max cDNA clone GENO 29 G192 AV423663 2.00E−46 Lotus japonicus AV423663 Lotus japonicus young plants (two- 29 G192 BM112869 1.00E−41 Solanum tuberosum EST560405 potato roots Solanum tuberosum 29 G192 BU837263 8.00E−39 Populus tremula x T096G05 Populus apica Populus tremuloides 29 G192 AAAA01003718 6.00E−34 Oryza sativa (indica ( ) scaffold003718 cultivar-group) 29 G192 AC018727 6.00E−34 Oryza sativa chromosome 10 clone OSJNBa0056G17, *** SEQUENC 29 G192 AP004683 1.00E−33 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 29 G192 AW447931 1.00E−32 Triticum aestivum BRY_1082 BRY Triticum aestivum cDNA clone 29 G192 gi12039364 1.90E−35 Oryza sativa putative DNA-binding protein. 29 G192 gi1432056 2.00E−24 Petroselinum crispum WRKY3. 29 G192 gi4894963 8.80E−24 Avena sativa DNA-binding protein WRKY3. 29 G192 gi4760596 1.80E−23 Nicotiana tabacum DNA-binding protein NtWRKY3. 29 G192 gi11993901 4.30E−20 Dactylis glomerata somatic embryogenesis related protein. 29 G192 gi21644680 1.60E−17 Oryza sativa (japonica hypothetical prote cultivar-group) 29 G192 gi23305051 5.00E−17 Oryza sativa (indica WRKY transcription f cultivar-group) 29 G192 gi1076685 1.90E−15 Ipomoea batatas SPF1 protein-sweet potato. 29 G192 gi7484759 2.30E−15 Cucumis sativus SP8 binding protein homolog-cucumber. 29 G192 gi3420906 5.10E−15 Pimpinella brachycarpa zinc finger protein; WRKY1. 31 G196 BH944961 9.00E−69 Brassica oleracea obu81g06.g1 B. oleracea002 Brassica olerac 31 G196 AAAA01003718 1.00E−46 Oryza sativa (indica ( ) scaffold003718 cultivar-group) 31 G196 AC018727 1.00E−46 Oryza sativa chromosome 10 clone OSJNBa0056G17, *** SEQUENC 31 G196 BI923235 6.00E−40 Lycopersicon EST543139 tomato callus esculentum Lycopersico 31 G196 BM113882 4.00E−38 Solanum tuberosum EST561418 potato roots Solanum tuberosum 31 G196 AW596933 1.00E−35 Glycine max sj84f07.y1 Gm-c1034 Glycine max cDNA clone GENO 31 G196 AV423663 2.00E−34 Lotus japonicus AV423663 Lotus japonicus young plants (two- 31 G196 BG647709 3.00E−34 Medicago truncatula EST509328 HOGA Medicago truncatula cDNA 31 G196 BQ855766 3.00E−33 Lactuca sativa QGB27K18.yg.ab1 QG_ABCDI lettuce salinas Lac 31 G196 BU837263 5.00E−32 Populus tremula x T096G05 Populus apica Populus tremuloides 31 G196 gi12039364 3.30E−51 Oryza sativa putative DNA-binding protein. 31 G196 gi4894963 2.40E−27 Avena sativa DNA-binding protein WRKY3. 31 G196 gi10798760 7.00E−26 Nicotiana tabacum WRKY transcription factor Nt-SubD48. 31 G196 gi1432056 6.20E−25 Petroselinum crispum WRKY3. 31 G196 gi11993901 3.00E−20 Dactylis glomerata somatic embryogenesis related protein. 31 G196 gi20160973 3.50E−20 Oryza sativa (japonica hypothetical prote cultivar-group) 31 G196 gi23305051 1.10E−14 Oryza sativa (indica WRKY transcription f cultivar-group) 31 G196 gi9187622 1.40E−14 Solanum tuberosum WRKY DNA binding protein. 31 G196 gi1076685 2.50E−14 Ipomoea batatas SPF1 protein-sweet potato. 31 G196 gi13620227 5.50E−14 Lycopersicon hypothetical protein. esculentum 33 G211 BG441912 6.00E−70 Gossypium arboreum GA_Ea0015B19f Gossypium arboreum 7-10 d 33 G211 AF336278 1.00E−69 Gossypium hirsutum BNLGHi233 (bnlghi6233) mRNA, complete cd 33 G211 BU837990 3.00E−66 Populus tremula x T108C04 Populus apica Populus tremuloides 33 G211 D88620 2.00E−57 Oryza sativa mRNA for OSMYB4, complete cds. 33 G211 AW186273 6.00E−54 Glycine max se65f12.y1 Gm-c1019 Glycine max cDNA clone GENO 33 G211 PMU39448 1.00E−52 Picea mariana MYB-like transcriptional factor MBF1 mRNA, co 33 G211 AAAA01005841 1.00E−52 Oryza sativa (indica ( ) scaffold005841 cultivar-group) 33 G211 BI674748 7.00E−52 Zea mays 949066G11.y2 949- Juvenile leaf and shoot cDNA fr 33 G211 AW775893 2.00E−51 Medicago truncatula EST334958 DSIL Medicago truncatula cDNA 33 G211 HVMYB2 2.00E−51 Hordeum vulgare H. vulgare myb2 mRNA. 33 G211 gi13346178 1.50E−67 Gossypium hirsutum BNLGHi233. 33 G211 gi22535556 1.10E−53 Oryza sativa (japonica myb-related protei cultivar-group) 33 G211 gi2605623 1.10E−53 Oryza sativa OSMYB4. 33 G211 gi1101770 5.70E−52 Picea mariana MYB-like transcriptional factor MBF1. 33 G211 gi82310 2.00E−51 Antirrhinum majus myb protein 330-garden snapdragon. 33 G211 gi127582 4.00E−51 Zea mays MYB-RELATED PROTEIN ZM38. 33 G211 gi19055 1.10E−50 Hordeum vulgare MybHv5. 33 G211 gi22795039 1.10E−50 Populus x canescens putative MYB transcription factor. 33 G211 gi1167484 3.60E−50 Lycopersicon transcription factor. esculentum 33 G211 gi20563 3.70E−50 Petunia x hybrida protein 1. 35 G214 PVU420902 1.00E−111 Phaseolus vulgaris mRNA for LHY protein. 35 G214 BU868664 6.00E−60 Populus balsamifera M118F07 Populus flow subsp. trichocarpa 35 G214 BE331563 2.00E−50 Glycine max sp15d08.y1 Gm-c1042 Glycine max cDNA clone GENO 35 G214 BH935194 1.00E−49 Brassica oleracea ode18e05.g1 B. oleracea002 Brassica olerac 35 G214 AAAA01009649 4.00E−49 Oryza sativa (indica ( ) scaffold009649 cultivar-group) 35 G214 AP004460 5.00E−48 Oryza sativa (japonica ( ) chromosome 8 clo cultivar-group) 35 G214 AW979367 2.00E−46 Lycopersicon EST310415 tomato root esculentum deficiency, C 35 G214 BM322287 5.00E−46 Sorghum bicolor PIC1_2_F02.b1_A002 Pathogen-infected compat 35 G214 AY103618 4.00E−45 Zea mays PCO118792 mRNA sequence. 35 G214 BG524104 3.00E−44 Stevia rebaudiana 38-82 Stevia field grown leaf cDNA Stevia 35 G214 gi21213868 7.60E−57 Phaseolus vulgaris LHY protein. 35 G214 gi15528628 2.40E−23 Oryza sativa hypothetical protein~similar to Oryza sativa 35 G214 gi12406993 1.20E−06 Hordeum vulgare MCB1 protein. 35 G214 gi20067661 1.40E−06 Zea mays one repeat myb transcriptional factor. 35 G214 gi18874263 3.70E−06 Antirrhinum majus MYB-like transcription factor DIVARICAT 35 G214 gi24850305 1.00−05 Oryza sativa (japonica transcription fact cultivar-group) 35 G214 gi12005328 3.00E−05 Hevea brasiliensis unknown. 35 G214 gi6688529 6.80E−05 Lycopersicon I-box binding factor. esculentum 35 G214 gi19911579 7.10E−05 Glycine max syringolide-induced protein 1-3-1B. 35 G214 gi7677132 0.0025 Secale cereale c-myb-like transcription factor. 37 G226 BU872107 2.00E−21 Populus balsamifera Q039C07 Populus flow subsp. trichocarpa 37 G226 BU831849 2.00E−21 Populus tremula x T026E01 Populus apica Populus tremuloides 37 G226 BM437313 9.00E−21 Vitis vinifera VVA017F06_54121 An expressed sequence tag da 37 G226 BI699876 1.00E−19 Glycine max sag49b09.y1 Gm-c1081 Glycine max cDNA clone GEN 37 G226 AL750151 4.00E−16 Pinus pinaster AL750151 AS Pinus pinaster cDNA clone AS06C1 37 G226 CA744013 2.00E−12 Triticum aestivum wri1s.pk006.m22 wri1s Triticum aestivum c 37 G226 BH961028 3.00E−12 Brassica oleracea odj30d06.g1 B. oleracea002 Brassica olerac 37 G226 BJ472717 8.00E−12 Hordeum vulgare subsp. BJ472717 K. Sato vulgare unpublished 37 G226 BF617445 8.00E−12 Hordeum vulgare HVSMEc0017G08f Hordeum vulgare seedling sho 37 G226 CA762299 2.00E−11 Oryza sativa (indica BR060003B10F03.ab1 IRR cultivar-group) 37 G226 gi9954118 2.20E−11 Solanum tuberosum tuber-specific and sucrose- responsive e 37 G226 gi14269333 2.50E−10 Gossypium raimondii myb-like transcription factor Myb 3. 37 G226 gi14269335 2.50E−10 Gossypium herbaceum myb-like transcription factor Myb 3. 37 G226 gi14269337 2.50E−10 Gossypium hirsutum myb-like transcription factor Myb 3. 37 G226 gi23476297 2.50E−10 Gossypioides kirkii myb-like transcription factor 3. 37 G226 gi15082210 8.50E−10 Fragaria x ananassa transcription factor MYB1. 37 G226 gi19072770 8.50E−10 Oryza sativa typical P-type R2R3 Myb protein. 37 G226 gi15042108 1.40E−09 Zea mays subsp. CI protein. parviglumis 37 G226 gi15042124 1.40E−09 Zea luxurians CI protein. 37 G226 gi20514371 1.40E−09 Cucumis sativus werewolf. 39 G241 AB028650 3.00E−69 Nicotiana tabacum mRNA for myb-related transcription factor 39 G241 PHMYBPH22 3.00E−68 Petunia x hybrida P. Hybrida myb.Ph2 gene encoding protein 39 G241 LETHM18GE 1.00E−65 Lycopersicon L. esculentum mRNA for esculentum myb-related 39 G241 AB073017 2.00E−63 Vitis labrusca x Vitis VlmybB1-2 gene for myb- vinifera rela 39 G241 OSMYB1202 5.00E−63 Oryza sativa O. sativa mRNA for myb factor, 1202 bp. 39 G241 AB029162 2.00E−62 Glycine max gene for GmMYB293, complete cds. 39 G241 BQ514539 1.00E−61 Solanum tuberosum EST621954 Generation of a set of potato c 39 G241 AW981167 5.00E−61 Medicago truncatula EST392361 DSIL Medicago truncatula cDNA 39 G241 BJ312394 4.00E−60 Triticum aestivum BJ312394 Y. Ogihara unpublished cDNA libr 39 G241 BM816803 2.00E−59 Hordeum vulgare HC114B11_SK.ab1 HC Hordeum vulgare cDNA clo 39 G241 gi6552361 1.50E−67 Nicotiana tabacum myb-related transcription factor LBM2. 39 G241 gi20561 8.30E−67 Petunia x hybrida protein 2. 39 G241 gi1370140 3.70E−64 Lycopersicon myb-related transcription esculentum factor. 39 G241 gi6492385 3.80E−62 Glycine max GmMYB29A2. 39 G241 gi1946265 2.70E−61 Oryza sativa myb. 39 G241 gi22266675 9.70E−57 Vitis labrusca x Vitis myb-related transcription vinifera 39 G241 gi127580 5.50E−54 Zea mays MYB-RELATED PROTEIN ZM1. 39 G241 gi11526779 9.90E−52 Zea mays subsp. P-like protein. parviglumis 39 G241 gi22795039 1.10E−48 Populus x canescens putative MYB transcription factor. 39 G241 gi13346188 1.40E−48 Gossypium hirsutum GHMYB25. 41 G248 BE642935 2.00E−25 Ceratopteris richardii Cri2_7_G20_SP6 Ceratopteris Spore Li 41 G248 AF190304 1.00E−24 Adiantum raddianum c-myb-like transcription factor (MYB3R-1 41 G248 AW040511 1.00E−24 Lycopersicon EST283471 tomato mixed esculentum elicitor, BT 41 G248 AF189786 2.00E−24 Physcomitrella patens putative c-myb-like transcription fac 41 G248 CA755789 4.00E−24 Oryza sativa (japonica BR030028000_PLATE_D1 cultivar-group) 41 G248 AB056123 2.00E−23 Nicotiana tabacum NtmybA2 mRNA for Myb, complete cds. 41 G248 AF189788 2.00E−22 Hordeum vulgare putative c-myb-like transcription factor (M 41 G248 AF236059 3.00E−22 Papaver rhoeas putative Myb-related domain (pmr) mRNA, part 41 G248 AF190302 2.00E−20 Secale cereale c-myb-like transcription factor (MYB3R-1) mR 41 G248 BH444284 1.00E−18 Brassica oleracea BOGON79TF BOGO Brassica oleracea genomic 41 G248 gi24417180 6.50E−28 Oryza sativa (japonica myb-like protein. cultivar-group) 41 G248 gi7677136 5.80E−27 Adiantum raddianum c-myb-like transcription factor. 41 G248 gi8745325 7.30E−25 Hordeum vulgare putative c-myb-like transcription factor. 41 G248 gi8745321 2.30E−24 Physcomitrella patens putative c-myb-like transcription f 41 G248 gi16326135 9.40E−23 Nicotiana tabacum Myb. 41 G248 gi7677132 1.50E−22 Secale cereale c-myb-like transcription factor. 41 G248 gi7630236 2.30E−22 Oryza sativa Similar to Arabidopsis thaliana chromosome 4 41 G248 gi7230673 7.10E−22 Papaver rhoeas putative Myb-related domain. 41 G248 gi14269337 1.50E−20 Gossypium hirsutum myb-like transcription factor Myb 3. 41 G248 gi14269333 1.60E−19 Gossypium raimondii myb-like transcription factor Myb 3. 43 G254 BU100118 4.00E−67 Triticum aestivum WHE3315_D06_H11ZS Chinese Spring wheat dr 43 G254 BI921951 1.00E−60 Lycopersicon EST541854 tomato callus esculentum Lycopersico 43 G254 AV909036 1.00E−57 Hordeum vulgare subsp. AV909036 K. Sato vulgare unpublished 43 G254 AW000459 9.00E−54 Zea mays 614016D07.y1 614-root cDNA library from Walbot L 43 G254 BG457702 2.00E−53 Medicago truncatula NF034C07PL1F1051 Phosphate starved leaf 43 G254 BU025460 2.00E−53 Helianthus annuus QHF9I05.yg.ab1 QH_EFGHJ sunflower RHA280 43 G254 BG593097 3.00E−52 Solanum tuberosum EST491775 cSTS Solanum tuberosum cDNA clo 43 G254 BU868480 3.00E−52 Populus balsamifera M116D03 Populus flow subsp. trichocarpa 43 G254 BU815973 5.00E−52 Populus tremula x N058E04 Populus bark Populus tremuloides 43 G254 BE330818 1.00E−51 Glycine max so85g03.y1 Gm-c1041 Glycine max cDNA clone GENO 43 G254 gi15528628 1.80E−25 Oryza sativa hypothetical protein~similar to Oryza sativa 43 G254 gi21213868 3.40E−24 Phaseolus vulgaris LHY protein. 43 G254 gi18461206 1.20E−07 Oryza sativa (japonica contains ESTs AU10 cultivar-group) 43 G254 gi12005328 1.10E−06 Hevea brasiliensis unknown. 43 G254 gi12406993 1.30E−06 Hordeum vulgare MCB1 protein. 43 G254 gi19911577 5.50E−06 Glycine max syringolide-induced protein 1-3-1A. 43 G254 gi6688529 3.90E−05 Lycopersicon I-box binding factor. esculentum 43 G254 gi18874265 3.90E−05 Antirrhinum majus MYB-like transcription factor DVL1. 43 G254 gi20067661 4.10E−05 Zea mays one repeat myb transcriptional factor. 43 G254 gi7705206 0.00072 Solanum tuberosum MybSt1. 45 G256 LETHM6 1.00E−78 Lycopersicon L. esculentum mRNA for esculentum myb-related t 45 G256 AY107969 4.00E−78 Zea mays PCO069276 mRNA sequence. 45 G256 BF270109 3.00E−76 Gossypium arboreum GA_Eb0006M14f Gossypium arboreum 7-10 d 45 G256 AW981415 5.00E−75 Medicago truncatula EST392568 DSIL Medicago truncatula cDNA 45 G256 BE342909 1.00E−72 Solanum tuberosum EST395753 potato stolon, Cornell Universi 45 G256 BQ623221 5.00E−72 Citrus sinensis USDA-FP_00312 Ridge pineapple sweet orange 45 G256 AP005636 1.00E−70 Oryza sativa (japonica ( ) chromosome 9 clo cultivar-group) 45 G256 AAAA01005623 1.00E−70 Oryza sativa (indica ( ) scaffold005623 cultivar-group) 454 G256 AC084762 8.00E−70 Oryza sativa chromosome 3 clone OSJNBa0013O08, *** SEQUENCI 45 G256 BM309647 8.00E−67 Glycine max sak65a08.y1 Gm-c1036 Glycine max cDNA clone SOY 45 G256 gi256828 1.10E−80 Antirrhinum majus Myb oncoprotein homolog {clone 306} [An 45 G256 gi1430848 8.20E−76 Lycopersicon transcription factor. esculentum 45 G256 gi18071376 6.80E−71 Oryza sativa putative transcription factor. 45 G256 gi23616974 3.60E−66 Oryza sativa (japonica contains EST C2815 cultivar-group) 45 G256 gi19072744 4.20E−65 Zea mays typical P-type R2R3 Myb protein. 45 G256 gi20563 7.30E−52 Petunia x hybrida protein 1. 45 G256 gi6552361 2.90E−50 Nicotiana tabacum myb-related transcription factor LBM2. 45 G256 gi13346188 2.30E−48 Gossypium hirsutum GHMYB25. 45 G256 gi5139802 4.70E−48 Glycine max GmMYB29A1. 45 G256 gi11526775 1.60E−47 Zea mays subsp. P2-t protein. parviglumis 47 G278 AF527176 1.0e−999 Brassica napus putative NPR1 (NPR1) mRNA, complete cds. 47 G278 BD064079 1.0e−999 Macadamia integrifolia Method for protecting plants. 47 G278 AF480488 1.00E−162 Nicotiana tabacum NPR1 mRNA, complete cds. 47 G278 AX351141 1.00E−106 Oryza sativa Sequence 15 from Patent WO0166755. 47 G278 AX041006 8.00E−97 Zea mays Sequence 1 from Patent WO0065037. 47 G278 AX351145 3.00E−95 Triticum aestivum Sequence 19 from Patent WO0166755. 47 G278 AC124609 2.00E−75 Medicago truncatula clone mth2-29b13, WORKING DRAFT SEQUENC 47 G278 AAAA01004121 6.00E−70 Oryza sativa (indica ( ) scaffold004121 cultivar-group) 47 G278 BZ056711 5.00E−67 Brassica oleracea lle49h07.b1 B. oleracea002 Brassica olerac 47 G278 BE435499 3.00E−50 Lycopersicon EST406577 tomato breaker esculentum fruit, TIG 47 G278 gi22003730 0.00E+00 Brassica napus putative NPR1. 47 G278 gi21552981 9.30E−155 Nicotiana tabacum NPR1. 47 G278 gi10934082 1.40E−128 Oryza sativa Arabidopsis thaliana regulatory protein NPR1 47 G278 gi18616499 5.00E−92 Triticum aestivum unnamed protein product. 47 G278 gi22535593 2.60E−88 Oryza sativa (japonica putative Regulator cultivar-group) 47 G278 gi11340603 3.40E−86 Zea mays unnamed protein product. 47 G278 gi17645766 0.00027 Glycine max unnamed protein product. 47 G278 gi549986 0.012 Pennisetum ciliare possible apospory- associated protein. 47 G278 gi18700703 0.14 Medicago sativa putative ankyrin-kinase. 47 G278 gi18700701 0.18 Medicago truncatula ankyrin-kinase. 49 G291 AF014375 1.00E−170 Medicago sativa putative JUN kinase activation domain bindi 49 G291 AF175964 1.00E−169 Lycopersicon JAB mRNA, complete cds. esculentum 49 G291 AF072849 1.00E−159 Oryza sativa subsp. jab1 protein (jab1) mRNA, indica comple 49 G291 AB055495 1.00E−159 Oryza sativa Jab1 mRNA for JUN- activation-domain-binding pr 49 G291 BG594615 1.00E−132 Solanum tuberosum EST493293 cSTS Solanum tuberosum cDNA clo 49 G291 BQ969736 1.00E−125 Helianthus annuus QHB39G11.yg.ab1 QH_ABCDI sunflower RHA801 49 G291 BQ871378 1.00E−123 Lactuca sativa QGI11K21.yg.ab1 QG_ABCDI lettuce salinas Lac 49 G291 BE036313 1.00E−115 Mesembryanthemum MO23B10 MO crystallinum Mesembryanthemum c 49 G291 BM066924 1.00E−113 Capsicum annuum KS07019G04 KS07 Capsicum annuum cDNA, mRNA 49 G291 BQ281547 1.00E−106 Triticum aestivum WHE3022_F07_K14ZS Wheat unstressed seedli 49 G291 gi3320379 1.80E−160 Medicago sativa putative JUN kinase activation domain bin 49 G291 gi12002865 3.00E−158 Lycopersicon JAB. esculentum 49 G291 gi17025926 4.30E−150 Oryza sativa JUN-activation-domain- binding protein homolo 49 G291 gi24636586 4.30E−150 Oryza sativa (japonica JUN-activation-dom cultivar-group) 49 G291 gi3420299 4.30E−150 Oryza sativa subsp. jab1 protein. indica 49 G291 gi13774977 0.73 Pinus mugo NADH dehydrogenase subunit 3. 49 G291 gi13774980 0.73 Pinus sylvestris NADH dehydrogenase subunit 3. 49 G291 gi13899006 0.89 Abies alba NADH dehydrogenase subunit 3. 49 G291 gi23503480 1 Glycine max heat shock protein DnaJ. 51 G303 BI677665 2.00E−40 Robinia pseudoacacia CLS342 CLS (Cambium and bark region of 51 G303 BQ995023 2.00E−38 Lactuca sativa QGF8N12.yg.ab1 QG_EFGHJ lettuce serriola Lac 51 G303 AAAA01003345 5.00E−36 Oryza sativa (indica ( ) scaffold003345 cultivar-group) 51 G303 AC121489 6.00E−36 Oryza sativa (japonica ( ) chromosome 3 clo cultivar-group) 51 G303 BE022329 6.00E−35 Glycine max sm73e05.y1 Gm-c1028 Glycine max cDNA clone GENO 51 G303 BI480474 2.00E−32 Triticum aestivum WHE2903_F02_L03ZS Wheat aluminum-stressed 51 G303 BH492255 7.00E−32 Brassica oleracea BOHLS25TR BOHL Brassica oleracea genomic 51 G303 BI128898 2.00E−30 Populus tremula x G083P21Y Populus camb Populus tremuloides 51 G303 CAR011013 1.00E−29 Cicer arietinum epicotyl EST, clone Can133. 51 G303 AW573949 4.00E−27 Medicago truncatula EST316540 GVN Medicago truncatula cDNA 51 G303 gi19920107 4.50E−43 Oryza sativa (japonica Putative helix-loo cultivar-group) 51 G303 gi3641870 4.30E−31 Cicer arietinum hypothetical protein. 51 G303 gi10998404 1.90E−09 Petunia x hybrida anthocyanin 1. 51 G303 gi18568238 2.10E−08 Zea mays regulatory protein. 51 G303 gi527661 2.90E−08 Phyllostachys acuta myc-like regulatory R gene product. 51 G303 gi1086538 6.10E−08 Oryza rufipogon transcriptional activator Rb homolog. 51 G303 gi527653 6.10E−08 Pennisetum glaucum myc-like regulatory R gene product. 51 G303 gi1086534 7.90E−08 Oryza officinalis transcriptional activator Ra homolog. 51 G303 gi1086540 1.90E−07 Oryza sativa Ra. 51 G303 gi527663 4.70E−07 Tripsacum australe myc-like regulatory R gene product. 53 G312 AAAA01008118 1.00E−137 Oryza sativa (indica ( ) scaffold008118 cultivar-group) 53 G312 BH521755 1.00E−69 Brassica oleracea BOHEY85TF BOHE Brassica oleracea genomic 53 G312 AW944694 4.00E−67 Euphorbia esula 00182 leafy spurge Lambda HybriZAP 2.1 two- 53 G312 BQ296629 3.00E−66 Glycine max san83a05.y2 Gm-c1052 Glycine max cDNA clone SOY 53 G312 BG446635 7.00E−64 Gossypium arboreum GA_Eb0036G15f Gossypium arboreum 7-10 d 53 G312 BH873477 8.00E−60 Zea mays hp45c06.b2 WGS-ZmaysF (JM107 adapted methyl filter 53 G312 BF257184 4.00E−56 Hordeum vulgare HVSMEf0012B22f Hordeum vulgare seedling roo 53 G312 AV414014 1.00E−52 Lotus japonicus AV414014 Lotus japonicus young plants (two- 53 G312 AF098674 4.00E−52 Lycopersicon lateral suppressor protein esculentum (Ls) mRN 53 G312 AB048713 2.00E−51 Pisum sativum PsSCR mRNA for SCARECROW, complete cds. 53 G312 gi13365610 1.30E−57 Pisum sativum SCARECROW. 53 G312 gi10178637 2.60E−53 Zea mays SCARECROW. 53 G312 gi13620224 1.30E−52 Lycopersicon lateral suppressor. esculentum 53 G312 gi13937306 4.80E−50 Oryza sativa gibberellin-insensitive protein OsGAI. 53 G312 gi20334379 1.80E−48 Vitis vinifera GAI-like protein 1. 53 G312 gi19571020 5.80E−48 Oryza sativa (japonica contains ESTs AU16 cultivar-group) 53 G312 gi13620166 4.20E−47 Capsella rubella hypothetical protein. 53 G312 gi13170126 1.30E−45 Brassica napus unnamed protein product. 53 G312 gi20257438 7.60E−44 Argyroxiphium GIA/RGA-li sandwicense subsp. macrocephalum 53 G312 gi20257420 9.60E−44 Dubautia arborea GIA/RGA-like gibberellin response modula 55 G325 AB001888 6.00E−41 Oryza sativa mRNA for zinc finger protein, complete cds, 55 G325 AAAA01003074 3.00E−32 Oryza sativa (indica ( ) scaffold003074 cultivar-group) 55 G325 BQ458955 2.00E−31 Hordeum vulgare HA02L20r HA Hordeum vulgare cDNA clone HA02 55 G325 AP005113 3.00E−31 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 55 G325 BJ209915 6.00E−31 Triticum aestivum BJ209915 Y. Ogihara unpublished cDNA libr 55 G325 BG644908 2.00E−30 Medicago truncatula EST506527 KV3 Medicago truncatula cDNA 55 G325 BG459023 2.00E−29 Zea mays 947052H08.y1 947-2 week shoot from Barkan lab Ze 55 G325 BQ121038 4.00E−29 Solanum tuberosum EST606614 mixed potato tissues Solanum tu 55 G325 AP004972 4.00E−29 Lotus japonicus genomic DNA, chromosome 3, clone: LjT41A07, 55 G325 BH926519 1.00E−28 Brassica oleracea odj42f08.b1 B. oleracea002 Brassica olerac 55 G325 gi3618320 9.80E−48 Oryza sativa zinc finger protein. 55 G325 gi3341723 1.70E−15 Raphanus sativus CONSTANS-like 1 protein. 55 G325 gi22854952 2.20E−15 Brassica nigra COL1 protein. 55 G325 gi2303683 2.00E−14 Brassica napus unnamed protein product. 55 G325 gi23495871 2.30E−13 Oryza sativa (japonica putative zinc-fing cultivar-group) 55 G325 gi4091806 3.80E−13 Malus x domestica CONSTANS-like protein 2. 55 G325 gi10946337 6.20E−13 Ipomoea nil CONSTANS-like protein. 55 G325 gi21667475 2.00E−11 Hordeum vulgare CONSTANS-like protein. 55 G325 gi4557093 1.10E−10 Pinus radiata zinc finger protein. 55 G325 gi21655154 1.20E−09 Hordeum vulgare subsp. CONSTANS-like protein vulgare CO5. 57 G343 AC069300 2.00E−50 Oryza sativa chromosome 10 clone OSJNBa0010C11, *** SEQUENC 57 G343 BU827056 4.00E−50 Populus tremula x UK127TH09 Populus api Populus tremuloides 57 G343 AAAA01001158 1.00E−47 Oryza sativa (indica ( ) scaffold001158 cultivar-group) 57 G343 BQ462644 3.00E−41 Hordeum vulgare HI01J05T HI Hordeum vulgare cDNA clone HI01 57 G343 AW235021 4.00E−41 Glycine max sf21h11.y1 Gm-c1028 Glycine max cDNA clone GENO 57 G343 BZ328210 8.00E−41 Zea mays id36b06.g1 WGS-ZmaysF (JM107 adapted methyl filter 57 G343 BH534811 1.00E−40 Brassica oleracea BOGJZ23TF BOGJ Brassica oleracea genomic 57 G343 BQ851743 2.00E−37 Lactuca sativa QGB16C22.yg.ab1 QG_ABCDI lettuce salinas Lac 57 G343 AW922818 5.00E−37 Sorghum bicolor DG1_46_F02.g1_A002 Dark Grown 1 (DG1) Sorgh 57 G343 AC132491 9.00E−37 Oryza sativa (japonica ( ) chromosome 5 clo cultivar-group) 57 G343 gi14165317 2.10E−57 Oryza sativa putative transcription factor. 57 G343 gi21902044 6.50E−45 Oryza sativa (japonica hypothetical prote cultivar-group) 57 G343 gi12711287 1.60E−31 Nicotiana tabacum GATA-1 zinc finger protein. 57 G343 gi1076609 4.40E−22 Nicotiana NTL1 protein-curled- plumbaginifolia leaved to 57 G343 gi20372847 0.34 Hordeum vulgare subsp. dof zinc finger protein. vulgare 57 G343 gi19322 0.41 Lycopersicon glycine-rich protein. esculentum 57 G343 gi21439754 0.55 Zea mays unnamed protein product. 57 G343 gi3219155 0.55 Mesembryanthemum transcription factor Vp1. crystallinum 57 G343 gi23504757 0.59 Pisum sativum nodule inception protein. 57 G343 gi21439770 0.67 Triticum aestivum unnamed protein product. 59 G353 BQ790831 5.00E−68 Brassica rapa subsp. E4675 Chinese cabbage pekinensis etiol 59 G353 BZ019752 1.00E−67 Brassica oleracea oed85c06.g1 B. oleracea002 Brassica olerac 59 G353 L46574 6.00E−40 Brassica rapa BNAF1975 Mustard flower buds Brassica rapa PL cD 59 G353 AB006601 7.00E−26 Petunia x hybrida mRNA for ZPT2-14, complete cds. 59 G353 BM437146 2.00E−25 Vitis vinifera VVA015A06_53787 An expressed sequence tag da 59 G353 BI422808 1.00E−24 Lycopersicon EST533474 tomato callus, esculentum TAMU Lycop 59 G353 BU867080 1.00E−24 Populus tremula x S074B01 Populus imbib Populus tremuloides 59 G353 BM527789 3.00E−23 Glycine max sal65h07.y1 Gm-c1061 Glycine max cDNA clone SOY 59 G353 BQ980246 5.00E−23 Lactuca sativa QGE10I12.yg.ab1 QG_EFGHJ lettuce serriola La 59 G353 BQ121106 2.00E−22 Solanum tuberosum EST606682 mixed potato tissues Solanum tu 59 G353 gi2346976 6.50E−28 Petunia x hybrida ZPT2-13. 59 G353 gi15623820 4.40E−25 Oryza sativa hypothetical protein. 59 G353 gi21104613 1.40E−18 Oryza sativa (japonica contains ESTs AU07 cultivar-group) 59 G353 gi485814 3.10E−13 Triticum aestivum WZF1. 59 G353 gi7228329 4.00E−12 Medicago sativa putative TFIIIA (or kruppel)-like zinc fi 59 G353 gi1763063 1.70E−11 Glycine max SCOF-1. 59 G353 gi2981169 2.60E−11 Nicotiana tabacum osmotic stress-induced zinc- finger prot 59 G353 gi4666360 1.10E−10 Datisca glomerata zinc-finger protein 1. 59 G353 gi2129892 2.30E−08 Pisum sativum probable finger protein Pszf1-garden pea. 59 G353 gi2058504 0.00018 Brassica rapa zinc-finger protein-1. 61 G354 BZ083260 5.00E−49 Brassica oleracea lle29f02.g1 B. oleracea002 Brassica olerac 61 G354 BQ790831 8.00E−45 Brassica rapa subsp. E4675 Chinese cabbage pekinensis etiol 61 G354 AB006600 6.00E−27 Petunia x hybrida mRNA for ZPT2-13, complete cds. 61 G354 L46574 1.00E−26 Brassica rapa BNAF1975 Mustard flower buds Brassica rapa cD 61 G354 BM437146 3.00E−24 Vitis vinifera VVA015A06_53787 An expressed sequence tag da 61 G354 BQ121105 6.00E−24 Solanum tuberosum EST606681 mixed potato tissues Solanum tu 61 G354 BM527789 2.00E−23 Glycine max sal65h07.y1 Gm-c1061 Glycine max cDNA clone SOY 61 G354 AI898309 2.00E−23 Lycopersicon EST267752 tomato ovary, esculentum TAMU Lycope 61 G354 BU867080 5.00E−22 Populus tremula x S074B01 Populus imbib Populus tremuloides 61 G354 BQ980246 1.00E−21 Lactuca sativa QGE10I12.yg.ab1 QG_EFGHJ lettuce serriola La 61 G354 gi2346976 5.60E−29 Petunia x hybrida ZPT2-13. 61 G354 gi15623820 1.90E−22 Oryza sativa hypothetical protein. 61 G354 gi21104613 4.00E−19 Oryza sativa (japonica contains ESTs AU07 cultivar-group) 61 G354 gi2981169 1.80E−17 Nicotiana tabacum osmotic stress-induced zinc- finger prot 61 G354 gi1763063 4.10E−16 Glycine max SCOF-1. 61 G354 gi4666360 8.90E−15 Datisca glomerata zinc-finger protein 1. 61 G354 gi2058504 1.00E−14 Brassica rapa zinc-finger protein-1. 61 G354 gi7228329 4.90E−14 Medicago sativa putative TFIIIA (or kruppel)-like zinc fi 61 G354 gi485814 3.20E−13 Triticum aestivum WZF1. 61 G354 gi2129892 1.20E−06 Pisum sativum probable finger protein Pszf1-garden pea. 63 G361 BG135559 1.00E−24 Lycopersicon EST468451 tomato crown esculentum gall Lycoper 63 G361 AW686309 4.00E−23 Medicago truncatula NF036D10NR1F1000 Nodulated root Medicag 63 G361 BU891880 8.00E−23 Populus tremula P056E03 Populus petioles cDNA library Popul 63 G361 BU877646 2.00E−22 Populus balsamifera V037D09 Populus flow subsp. trichocarpa 63 G361 BH725134 9.00E−22 Brassica oleracea BOHWL71TF BO_2_3_KB Brassica oleracea gen 63 G361 BI426538 2.00E−21 Glycine max sag04d12.y1 Gm-c1080 Glycine max cDNA clone GEN 63 G361 AP003214 2.00E−21 Oryza sativa chromosome 1 clone OSJNBa0083M16, *** SEQUENCI 63 G361 AAAA01004859 3.00E−21 Oryza sativa (indica ( ) scaffold004859 cultivar-group) 63 G361 BU494379 1.00E−20 Lotus japonicus Ljirnpest50-154-h2 Ljirnp Lambda HybriZap t 63 G361 BQ488216 2.00E−17 Beta vulgaris 35-E8143-006-003-J02-T3 Sugar beet MPIZ-ADIS- 63 G361 gi15528588 4.00E−29 Oryza sativa hypothetical protein. 63 G361 gi18390109 2.80E−13 Sorghum bicolor putative zinc finger protein. 63 G361 gi18674684 1.50E−07 Zea ramosa unnamed protein product. 63 G361 gi14275902 6.10E−07 Petunia x hybrida lateral shoot inducing factor. 63 G361 gi21104613 0.00024 Oryza sativa (japonica contains ESTs AU07 cultivar-group) 63 G361 gi2129892 0.00062 Pisum sativum probable finger protein Pszf1-garden pea. 63 G361 gi2058504 0.0018 Brassica rapa zinc-finger protein-1. 63 G361 gi4666360 0.018 Datisca glomerata zinc-finger protein 1. 63 G361 gi7228329 0.047 Medicago sativa putative TFIIIA (or kruppel)-like zinc fi 63 G361 gi1763063 0.084 Glycine max SCOF-1. 65 G362 BF645161 6.00E−21 Medicago truncatula NF031C06EC1F1049 Elicited cell culture 65 G362 BI206903 6.00E−21 Lycopersicon EST524943 cTOS esculentum Lycopersicon esculen 65 G362 BG047435 1.00E−18 Glycine max saa71c12.y1 Gm-c1060 Glycine max cDNA clone GEN 65 G362 BU877646 2.00E−15 Populus balsamifera V037D09 Populus flow subsp. trichocarpa 65 G362 BU891880 2.00E−15 Populus tremula P056E03 Populus petioles cDNA library Popul 65 G362 AP003214 3.00E−13 Oryza sativa chromosome 1 clone OSJNBa0083M16, *** SEQUENCI 65 G362 AAAA01004859 3.00E−13 Oryza sativa (indica ( ) scaffold004859 cultivar-group) 65 G362 BE358938 2.00E−11 Sorghum bicolor DG1_37_E12.b1_A002 Dark Grown 1 (DG1) Sorgh 65 G362 BQ488435 2.00E−11 Beta vulgaris 05-E8886-006-003-J02-T3 Sugar beet MPIZ-ADIS- 65 G362 BU494379 3.00E−11 Lotus japonicus Ljirnpest50-154-h2 Ljirnp Lambda HybriZap t 65 G362 gi15528588 2.70E−18 Oryza sativa hypothetical protein. 65 G362 gi2346984 9.00E−09 Petunia x hybrida ZPT2-9. 65 G362 gi18390109 9.90E−08 Sorghum bicolor putative zinc finger protein. 65 G362 gi21104613 0.00015 Oryza sativa (japonica contains ESTs AU07 cultivar-group) 65 G362 gi18674684 0.0028 Zea ramosa unnamed protein product. 65 G362 gi7228329 0.0029 Medicago sativa putative TFIIIA (or kruppel)-like zinc fi 65 G362 gi1763063 0.0039 Glycine max SCOF-1. 65 G362 gi485814 0.0062 Triticum aestivum WZF1. 65 G362 gi4666360 0.0072 Datisca glomerata zinc-finger protein 1. 65 G362 gi2058504 0.019 Brassica rapa zinc-finger protein-1. 67 G371 CA799489 2.00E−38 Glycine max sat34e06.y1 Gm-c1056 Glycine max cDNA clone SOY 67 G371 AF265664 2.00E−32 Solanum tuberosum resistance gene cluster, complete sequenc 67 G371 AJ497824 2.00E−31 Medicago truncatula AJ497824 MTFLOW Medicago truncatula cDN 67 G371 AY129244 4.00E−31 Populus x canescens putative RING protein (RING) mRNA, comp 67 G371 BM985575 1.00E−30 Thellungiella halophila 1_F12_T3 Ath Thellungiella halophil 67 G371 BF051105 2.00E−30 Lycopersicon EST436280 tomato esculentum developing/immatur 67 G371 BU834871 2.00E−30 Populus tremula x T066G02 Populus apica Populus tremuloides 67 G371 BM300635 5.00E−25 Mesembryanthemum MCA054H03_21640 Ice crystallinum plant Lam 67 G371 BQ586594 1.00E−24 Beta vulgaris E012388-024-012-I21-SP6 MPIZ-ADIS-024-leaf Be 67 G371 BU880207 1.00E−24 Populus balsamifera UM42TH03 Populus flo subsp. trichocarpa 67 G371 gi22795037 8.80E−24 Populus x canescens putative RING protein. 67 G371 gi15289911 2.20E−21 Oryza sativa hypothetical protein~similar to Arabidopsis 67 G371 gi22535577 2.20E−21 Oryza sativa (japonica hypothetical prote cultivar-group) 67 G371 gi7688063 0.00026 Pisum sativum constitutively photomorphogenic 1 protein. 67 G371 gi18129286 0.0057 Pinus pinaster putative RING zinc finger protein. 67 G371 gi22775495 0.014 Arabis gemmifera similar to A. thaliana AT4g08590. 67 G371 gi15029364 0.015 Rosa hybrid cultivar photoregulatory zinc-finger protein 67 G371 gi7592844 0.025 Oryza sativa subsp. COP1. japonica 67 G371 gi25044835 0.059 Ananas comosus RING zinc finger protein. 67 G371 gi11127996 0.12 Ipomoea nil COP1. 69 G390 AB084381  1.0e−999 Zinnia elegans ZeHB-11 mRNA for homoeobox leucine-zipper pr 69 G390 AB032182  1.0e−999 Physcomitrella patens PpHB10 mRNA for homeobox protein PpHB 69 G390 AY105765  1.0e−999 Zea mays PCO144112 mRNA sequence. 69 G390 AAAA01006159  1.0e−999 Oryza sativa (indica ( ) scaffold006159 cultivar-group) 69 G390 AP003197 1.00E−177 Oryza sativa chromosome 1 clone B1015E06, *** SEQUENCING IN 69 G390 BQ857624 1.00E−106 Lactuca sativa QGB8A10.yg.ab1 QG_ABCDI lettuce salinas Lact 69 G390 BI925551 1.00E−101 Lycopersicon EST545440 tomato flower, esculentum buds 0-3 m 69 G390 AW686191 1.00E−100 Medicago truncatula NF035A10NR1F1000 Nodulated root Medicag 69 G390 CA032516 1.00E−90 Hordeum vulgare subsp. HX13F16r HX Hordeum vulgare vulgare 69 G390 BQ116871 8.00E−90 Solanum tuberosum EST602447 mixed potato tissues Solanum tu 69 G390 gi24417149 1.00E−299 Zinnia elegans homoeobox leucine-zipper protein. 69 G390 gi13384370 8.40E−280 Oryza sativa putative homeodomain- leucine zipper protein. 69 G390 gi24431605 4.10E−274 Oryza sativa (japonica Putative homeodoma cultivar-group) 69 G390 gi7209912 2.80E−244 Physcomitrella patens homeobox protein PpHB10. 69 G390 gi3868829 4.50E−32 Ceratopteris richardii CRHB1. 69 G390 gi19070143 5.00E−22 Picea abies homeodomain protein HB2. 69 G390 gi1173622 1.10E−21 Phalaenopsis sp. homeobox protein. SM9108 69 G390 gi2147484 1.10E−21 Phalaenopsis sp. homeotic protein, ovule- specific-Phala 69 G390 gi8920427 2.30E−20 Zea mays OCL5 protein. 69 G390 gi18481701 7.70E−19 Sorghum bicolor OCL5 protein. 71 G391 AB084381  1.0e−999 Zinnia elegans ZeHB-11 mRNA for homoeobox leucine-zipper pr 71 G391 AB032182  1.0e−999 Physcomitrella patens PpHB10 mRNA for homeobox protein PpHB 71 G391 AY105765  1.0e−999 Zea mays PCO144112 mRNA sequence. 71 G391 AAAA01006159 1.00E−146 Oryza sativa (indica ( ) scaffold006159 cultivar-group) 71 G391 BQ857624 1.00E−111 Lactuca sativa QGB8A10.yg.ab1 QG_ABCDI lettuce salinas Lact 71 G391 AP003197 1.00E−106 Oryza sativa chromosome 1 clone B1015E06, *** SEQUENCING IN 71 G391 BI925551 1.00E−102 Lycopersicon EST545440 tomato flower, esculentum buds 0-3 m 71 G391 AW686191 1.00E−102 Medicago truncatula NF035A10NR1F1000 Nodulated root Medicag 71 G391 CA032516 1.00E−92 Hordeum vulgare subsp. HX13F16r HX Hordeum vulgare vulgare 71 G391 BQ116871 6.00E−91 Solanum tuberosum EST602447 mixed potato tissues Solanum tu 71 G391 gi24417149  5.3e−310 Zinnia elegans homoeobox leucine-zipper protein. 71 G391 gi13384370 3.20E−296 Oryza sativa putative homeodomain- leucine zipper protein. 71 G391 gi24431605 7.10E−283 Oryza sativa (japonica Putative homeodoma cultivar-group) 71 G391 gi7209912 4.60E−255 Physcomitrella patens homeobox protein PpHB10. 71 G391 gi3868829 6.30E−33 Ceratopteris richardii CRHB1. 71 G391 gi18481701 9.10E−24 Sorghum bicolor OCL5 protein. 71 G391 gi12002853 3.50E−23 Picea abies homeobox 1. 71 G391 gi1173622 1.20E−22 Phalaenopsis sp. homeobox protein. SM9108 71 G391 gi2147484 1.20E−22 Phalaenopsis sp. homeotic protein, ovule- specific-Phala 71 G391 gi8920427 9.30E−22 Zea mays OCL5 protein. 73 G409 BG044206 2.00E−66 Glycine max saa25c02.y1 Gm-c1059 Glycine max cDNA clone GEN 73 G409 AF443621 3.00E−66 Craterostigma homeodomain leucine plantagineum zipper prote 73 G409 AW220361 6.00E−60 Lycopersicon EST302844 tomato root esculentum during/after 73 G409 AF402606 5.00E−58 Phaseolus vulgaris homeodomain leucine zipper protein HDZ3 73 G409 AY105265 2.00E−56 Zea mays PCO062717 mRNA sequence. 73 G409 BQ165293 2.00E−51 Medicago truncatula EST611162 KVKC Medicago truncatula cDNA 73 G409 BH570275 1.00E−50 Brassica oleracea BOHAF65TF BOHA Brassica oleracea genomic 73 G409 BF620380 1.00E−48 Hordeum vulgare HVSMEc0019K16f Hordeum vulgare seedling sho 73 G409 BF588126 2.00E−48 Sorghum propinquum FM1_38_A10.b1_A003 Floral-Induced Merist 73 G409 AF145729 5.00E−45 Oryza sativa homeodomain leucine zipper protein (hox5) mRNA 73 G409 gi18034441 4.10E−65 Craterostigma homeodomain leucine plantagineum zipper pro 73 G409 gi15148920 1.10E−57 Phaseolus vulgaris homeodomain leucine zipper protein HDZ 73 G409 gi5006855 7.20E−45 Oryza sativa homeodomain leucine zipper protein. 73 G409 gi1435021 9.00E−38 Daucus carota DNA-binding protein. 73 G409 gi6018089 1.50E−37 Glycine max homeodomain-leucine zipper protein 57. 73 G409 gi1161575 2.20E−36 Lycopersicon homeobox. esculentum 73 G409 gi11231065 1.40E−34 Zinnia elegans homeobox-leucine zipper protein. 73 G409 gi7415614 1.40E−34 Physcomitrella patens homeobox protein PpHB1. 73 G409 gi8133126 4.10E−33 Brassica rapa subsp. hb-6-like protein. pekinensis 73 G409 gi22651698 1.80E−32 Nicotiana tabacum homeodomain protein Hfi22. 75 G427 MDKNOX1 1.00E−143 Malus domestica M. domestica mRNA for knotted1-like homeobox 75 G427 AB004797 1.00E−136 Nicotiana tabacum NTH23 mRNA, complete cds. 75 G427 LEU76409 1.00E−132 Lycopersicon homeobox 1 protein esculentum (THox1) mRNA, pa 75 G427 AB043957 1.00E−118 Ceratopteris richardii mRNA for CRKNOX3, complete cds. 75 G427 AW560103 1.00E−115 Medicago truncatula EST315151 DSIR Medicago truncatula cDNA 75 G427 AB061818 1.00E−112 Oryza sativa HOS59 mRNA for KNOX family class 2 homeodomain 75 G427 BQ873924 1.00E−100 Lactuca sativa QGI2O22.yg.ab1 QG_ABCDI lettuce salinas Lact 75 G427 BNHDIBOX 9.00E−99 Brassica napus B. napus hd1 mRNA for homeodomain-containing 75 G427 AY104273 8.00E−93 Zea mays PCO147946 mRNA sequence. 75 G427 BM063854 1.00E−91 Capsicum annuum KS01060C11 KS01 Capsicum annuum cDNA, mRNA 75 G427 gi1946222 5.10E−131 Malus domestica knotted1-like homeobox protein. 75 G427 gi3116212 3.40E−125 Nicotiana tabacum homeobox gene. 75 G427 gi4098244 8.10E−124 Lycopersicon homeobox 1 protein. esculentum 75 G427 gi1805618 3.60E−121 Oryza sativa OSH45 transcript. 75 G427 gi11463943 2.50E−113 Ceratopteris richardii CRKNOX3. 75 G427 gi1076449 1.40E−94 Brassica napus homeodomain-containing protein-rape. 75 G427 gi14348597 1.00E−93 Physcomitrella patens class 2 KNOTTED1-like protein MKN1- 75 G427 gi6016216 2.80E−43 Zea mays HOMEOBOX PROTEIN KNOTTED-1 LIKE 2. 75 G427 gi20977642 1.70E−34 Helianthus annuus knotted-1-like protein 1. 75 G427 gi3327269 6.50E−34 Ipomoea nil PKn1. 77 G438 ZEL312053  1.0e−999 Zinnia elegans mRNA for HD-Zip protein (hb1 gene). 77 G438 AB032182  1.0e−999 Physcomitrella patens PpHB10 mRNA for homeobox protein PpHB 77 G438 AY105765  1.0e−999 Zea mays PCO144112 mRNA sequence. 77 G438 AAAA01006159 1.00E−165 Oryza sativa (indica ( ) scaffold006159 cultivar-group) 77 G438 BU002601 1.00E−120 Lactuca sativa QGG31N03.yg.ab1 QG_EFGHJ lettuce serriola La 77 G438 BE035416 1.00E−106 Mesembryanthemum MO05A06 MO crystallinum Mesembryanthemum c 77 G438 BQ578798 1.00E−104 Triticum aestivum WHE0309_H06_O11ZS Wheat unstressed seedli 77 G438 BU927293 1.00E−103 Glycine max sas97g12.y1 Gm-c1036 Glycine max cDNA clone SOY 77 G438 AW696625 1.00E−102 Medicago truncatula NF109B06ST1F1048 Developing stem Medica 77 G438 BU041905 7.00E−89 Prunus persica PP_LEa0010O09f Peach developing fruit mesoca 77 G438 gi18076736  1.0e−999 Zinnia elegans HD-Zip protein. 77 G438 gi13384370  1.0e−999 Oryza sativa putative homeodomain- leucine zipper protein. 77 G438 gi24431605  3.3e−317 Oryza sativa (japonica Putative homeodoma cultivar-group) 77 G438 gi7209912 4.90E−238 Physcomitrella patens homeobox protein PpHB10. 77 G438 gi3868829 3.40E−35 Ceratopteris richardii CRHB1. 77 G438 gi18481701 4.00E−21 Sorghum bicolor OCL5 protein. 77 G438 gi1173622 8.50E−21 Phalaenopsis sp. homeobox protein. SM9108 77 G438 gi2147484 8.50E−21 Phalaenopsis sp. homeotic protein, ovule- specific-Phala 77 G438 gi12002853 1.40E−20 Picea abies homeobox 1. 77 G438 gi8920427 3.20E−20 Zea mays OCL5 protein. 79 G450 BQ155060 2.00E−84 Medicago truncatula NF075G11IR1F1088 Irradiated Medicago tr 79 G450 PTR306829 5.00E−83 Populus tremula x mRNA for aux/IAA pro Populus tremuloides 79 G450 BE053029 1.00E−81 Gossypium arboreum GA_Ea0031O18f Gossypium arboreum 7-10 d 79 G450 BI179192 1.00E−79 Solanum tuberosum EST520137 cSTE Solanum tuberosum cDNA clo 79 G450 BU006959 5.00E−78 Lactuca sativa QGH12O02.yg.ab1 QG_EFGHJ lettuce serriola La 79 G450 AF123508 8.00E−75 Nicotiana tabacum Nt-iaa28 deduced protein mRNA, complete c 79 G450 BQ623078 2.00E−72 Citrus sinensis USDA-FP_00169 Ridge pineapple sweet orange 79 G450 BI470140 7.00E−72 Glycine max sah88c10.y1 Gm-c1050 Glycine max cDNA clone GEN 79 G450 BU892057 7.00E−72 Populus tremula P058G09 Populus petioles cDNA library Popul 79 G450 AA427337 4.00E−71 Pisum sativum P482 Whero seedling lambda ZapII cDNA library 79 G450 gi20385508 4.20E−79 Populus tremula x auxin-regulated pro Populus tremuloides 79 G450 gi4887020 2.90E−73 Nicotiana tabacum Nt-iaa28 deduced protein. 79 G450 gi114734 1.10E−69 Glycine max AUXIN-INDUCED PROTEIN AUX28. 79 G450 gi22725714 2.00E−65 Mirabilis jalapa auxin-responsive protein IAA1; MjAux/IAA 79 G450 gi17976835 2.10E−61 Pinus pinaster putative auxin induced transcription facto 79 G450 gi6136832 4.20E−57 Cucumis sativus CS-IAA2. 79 G450 gi20257219 1.80E−56 Zinnia elegans auxin-regulated protein. 79 G450 gi17154533 2.10E−54 Oryza sativa putative IAA1 protein. 79 G450 gi22531416 5.30E−47 Gossypium hirsutum IAA16 protein. 79 G450 gi21104740 1.00E−43 Oryza sativa (japonica contains EST AU091 cultivar-group) 81 G464 BH998146 2.00E−50 Brassica oleracea oef97f09.g1 B. oleracea002 Brassica olerac 81 G464 BU043737 2.00E−44 Prunus persica PP_LEa0017A10f Peach 81developing fruit mesoca 81 G464 PTR306828 5.00E−44 Populus tremula x mRNA for aux/IAA pro Populus tremuloides 81 G464 BI207567 6.00E−44 Lycopersicon EST525607 cTOS esculentum Lycopersicon esculen 81 G464 BQ592350 1.00E−35 Beta vulgaris E012681-024-020-J14-SP6 MPIZ-ADIS-024-develop 81 G464 AV933892 4.00E−35 Hordeum vulgare subsp. AV933892 K. Sato vulgare unpublished 81 G464 BQ505545 5.00E−35 Solanum tuberosum EST612960 Generation of a set of potato c 81 G464 BE364015 3.00E−34 Sorghum bicolor PI1_11_G02.b1_A002 Pathogen induced 1 (PI1) 81 G464 BI118786 3.00E−34 Oryza sativa EST174 Differentially expressed cDNA libraries 81 G464 AI725624 9.00E−32 Gossypium hirsutum BNLGHi12459 Six-day Cotton fiber Gossypi 81 G464 gi20269057 1.60E−38 Populus tremula x aux/IAA protein. Populus tremuloides 81 G464 gi17976835 5.40E−32 Pinus pinaster putative auxin induced transcription facto 81 G464 gi5139697 2.00E−30 Cucumis sativus expressed in cucumber hypocotyls. 81 G464 gi22725714 6.30E−30 Mirabilis jalapa auxin-responsive protein IAA1; MjAux/IAA 81 G464 gi17154533 1.30E−29 Oryza sativa putative IAA1 protein. 81 G464 gi20257219 4.40E−29 Zinnia elegans auxin-regulated protein. 81 G464 gi2388689 4.40E−29 Glycine max GH1 protein. 81 G464 gi16610193 1.10E−27 Nicotiana tabacum IAA9 protein. 81 G464 gi1352057 3.60E−27 Pisum sativum AUXIN-INDUCED PROTEIN IAA4. 81 G464 gi21104740 5.80E−27 Oryza sativa (japonica contains EST AU091 cultivar-group) 83 G470 AB071293  1.0e−999 Oryza sativa OsARF2 mRNA for auxin response factor 2, parti 83 G470 OSA306306  1.0e−999 Oryza sativa (japonica Oryza sativa subsp. cultivar-group) 83 G470 AC126794  1.0e−999 Medicago truncatula clone mth2-24j7, WORKING DRAFT SEQUENCE 83 G470 AY106228 1.00E−131 Zea mays PCO137716 mRNA sequence. 83 G470 BQ578824 1.00E−118 Triticum aestivum WHE0407_B10_D19ZS Wheat etiolated seedlin 83 G470 BG045095 1.00E−108 Glycine max saa36f10.y1 Gm-c1059 Glycine max cDNA clone GEN 83 G470 CA030942 1.00E−102 Hordeum vulgare subsp. HX08J07r HX Hordeum vulgare vulgare 83 G470 BI098203 4.00E−96 Sorghum bicolor IP1_29_D05.b1_A002 Immature pannicle 1 (IP1 83 G470 BG886848 5.00E−96 Solanum tuberosum EST512699 cSTD Solanum tuberosum cDNA clo 83 G470 AI774352 7.00E−95 Lycopersicon EST255368 tomato esculentum resistant, Cornell 83 G470 gi20805236 8.60E−223 Oryza sativa (japonica auxin response fac cultivar-group) 83 G470 gi19352039 6.10E−222 Oryza sativa auxin response factor 2. 83 G470 gi24785191 7.00E−70 Nicotiana tabacum hypothetical protein. 83 G470 gi23343944 5.70E−16 Mirabilis jalapa auxin-responsive factor protein. 83 G470 gi20269053 1.70E−08 Populus tremula x aux/IAA protein. Populus tremuloides 83 G470 gi6136834 4.80E−07 Cucumis sativus CS-IAA3. 83 G470 gi287566 2.50E−06 Vigna radiata ORF. 83 G470 gi16610209 5.20E−06 Physcomitrella patens IAA/AUX protein. 83 G470 gi114733 8.60E−06 Glycine max AUXIN-INDUCED PROTEIN AUX22. 83 G470 gi18697008 4.00E−05 Zea mays unnamed protein product. 85 G477 BH981212 8.00E−48 Brassica oleracea odf77g01.b1 B. oleracea002 Brassica olerac 85 G477 BI925786 5.00E−39 Lycopersicon EST545675 tomato flower, esculentum buds 0-3 m 85 G477 BM408208 7.00E−38 Solanum tuberosum EST582535 potato roots Solanum tuberosum 85 G477 BQ874863 1.00E−30 Lactuca sativa QGI6H22.yg.ab1 QG_ABCDI lettuce salinas Lact 85 G477 AMA011622 4.00E−30 Antirrhinum majus mRNA for squamosa promoter binding 85 G477 BQ594361 4.00E−30 Beta vulgaris S015246-024-024-K10-SP6 MPIZ-ADIS-024-develop 85 G477 CA516258 1.00E−28 Capsicum annuum KS09055D03 KS09 Capsicum annuum cDNA, mRNA 85 G477 BU828403 2.00E−28 Populus tremula x K022P59P Populus apic Populus tremuloides 85 G477 BG442540 2.00E−28 Gossypium arboreum GA_Ea0017G06f Gossypium arboreum 7-10 d 85 G477 AW331087 7.00E−28 Zea mays 707047A12.x1 707-Mixed adult tissues from Walbot 85 G477 gi5931641 9.90E−32 Antirrhinum majus squamosa promoter binding protein-homol 85 G477 gi5931784 1.50E−28 Zea mays SBP-domain protein 4. 85 G477 gi8468036 4.40E−28 Oryza sativa Similar to Arabidopsis thaliana chromosome 2 85 G477 gi9087308 1.20E−14 Mitochondrion Beta orf102a. vulgaris var. altissima 85 G477 gi23630509 0.78 Triticum aestivum zinc finger protein. 85 G477 gi14597634 1 Physcomitrella patens 15_ppprotl_080_c02. 87 G481 BU238020 9.00E−71 Descurainia sophia Ds01_14a12_A Ds01_AAFC_ECORC_cold_stress 87 G481 BG440251 2.00E−56 Gossypium arboreum GA_Ea0006K20f Gossypium arboreum 7-10 d 87 G481 BF071234 1.00E−54 Glycine max st06h05.y1 Gm-c1065 Glycine max cDNA clone GENO 87 G481 BQ799965 2.00E−54 Vitis vinifera EST 2134 Green Grape berries Lambda Zap II L 87 G481 BQ488908 5.00E−53 Beta vulgaris 95-E9134-006-006-M23-T3 Sugar beet MPIZ-ADIS- 87 G481 BU499457 1.00E−52 Zea mays 946175D02.y1 946-tassel primordium prepared by S 87 G481 AI728916 2.00E−52 Gossypium hirsutum BNLGHi12022 Six-day Cotton fiber Gossypi 87 G481 BG642751 3.00E−52 Lycopersicon EST510945 tomato esculentum shoot/meristem Lyc 87 G481 BQ857127 3.00E−51 Lactuca sativa QGB6K24.yg.ab1 QG_ABCDI lettuce salinas Lact 87 G481 BE413647 6.00E−51 Triticum aestivum SCU001.E10.R990714 ITEC SCU Wheat Endospe 87 G481 gi115840 1.90E−51 Zea mays CCAAT-BINDING TRANSCRIPTION FACTOR SUBUNIT A (CB 87 G481 gi20160792 2.60E−47 Oryza sativa (japonica putative CAAT-box cultivar-group) 87 G481 gi15408794 7.10E−38 Oryza sativa putative CCAAT-binding transcription factor 87 G481 gi22536010 3.20E−35 Phaseolus coccineus LEC1-like protein. 87 G481 gi16902054 1.80E−32 Vernonia galamensis CCAAT-box binding factor HAP3 B domai 87 G481 gi16902050 6.10E−32 Glycine max CCAAT-box binding factor HAP3 B domain. 87 G481 gi16902056 1.60E−31 Argemone mexicana CCAAT-box binding factor HAP3 B domain. 87 G481 gi16902058 2.20E−27 Triticum aestivum CCAAT-box binding factor HAP3 B domain. 87 G481 gi388257 0.26 Lycopersicon glycine-rich protein. esculentum 87 G481 gi18266049 0.92 Brassica oleracea myrosinase precursor. 89 G482 BQ505706 7.00E−59 Solanum tuberosum EST613121 Generation of a set of potato c 89 G482 AC122165 6.00E−57 Medicago truncatula clone mth2-32m22, WORKING DRAFT SEQUENC 89 G482 BQ104671 2.00E−55 Rosa hybrid cultivar fc0546.e Rose Petals (Fragrant Cloud) 89 G482 BI469382 4.00E−55 Glycine max sai11b10.y1 Gm-c1053 Glycine max cDNA clone GEN 89 G482 AAAA01003638 1.00E−54 Oryza sativa (indica ( ) scaffold003638 cultivar-group) 89 G482 AP005193 1.00E−54 Oryza sativa (japonica ( ) chromosome 7 clo cultivar-group) 89 G482 BU880488 1.00E−53 Populus balsamifera UM49TG09 Populus flo subsp. trichocarpa 89 G482 BJ248969 2.00E−53 Triticum aestivum BJ248969 Y. Ogihara unpublished cDNA libr 89 G482 AC120529 4.00E−53 Oryza sativa chromosome 3 clone OSJNBa0039N21, *** SEQUENCI 89 G482 BU896236 7.00E−53 Populus tremula x X037F04 Populus wood Populus tremuloides 89 G482 gi115840 1.40E−46 Zea mays CCAAT-BINDING TRANSCRIPTION FACTOR SUBUNIT A (CB 89 G482 gi20160792 2.30E−41 Oryza sativa (japonica putative CAAT-box cultivar-group) 89 G482 gi22536010 9.00E−38 Phaseolus coccineus LEC1-like protein. 89 G482 gi15408794 1.50E−37 Oryza sativa putative CCAAT-binding transcription factor 89 G482 gi16902054 7.50E−34 Vernonia galamensis CCAAT-box binding factor HAP3 B domai 89 G482 gi16902050 5.30E−33 Glycine max CCAAT-box binding factor HAP3 B domain. 89 G482 gi16902056 4.80E−32 Argemone mexicana CCAAT-box binding factor HAP3 B domain. 89 G482 gi16902058 1.10E−30 Triticum aestivum CCAAT-box binding factor HAP3 B domain. 89 G482 gi100582 0.0018 Hordeum vulgare glycine-rich protein precursor-barley. 89 G482 gi7024451 0.0025 Citrus unshiu glycine-rich RNA-binding protein. 91 G484 BQ412047 3.00E−68 Gossypium arboreum GA_Ed0053D06r Gossypium arboreum 7-10 d 91 G484 AF464906 5.00E−67 Glycine max repressor protein (Dr1) mRNA, complete cds. 91 G484 AW719575 2.00E−64 Lotus japonicus LjNEST6a11r Lotus japonicus nodule library, 91 G484 BG648823 4.00E−64 Medicago truncatula EST510442 HOGA Medicago truncatula cDNA 91 G484 BQ593791 4.00E−64 Beta vulgaris E012763-024-026-O09-SP6 MPIZ-ADIS-024-develop 91 G484 BM436739 9.00E−64 Vitis vinifera VVA009B06_53061 An expressed sequence tag da 91 G484 BF113032 1.00E−63 Lycopersicon EST440542 tomato breaker esculentum fruit Lyco 91 G484 BG593107 7.00E−63 Solanum tuberosum EST491785 cSTS Solanum tuberosum cDNA clo 91 G484 BU014508 1.00E−61 Lactuca sativa QGJ7I14.yg.ab1 QG_EFGHJ lettuce serriola Lac 91 G484 AF464902 5.00E−59 Oryza sativa repressor protein (Dr1) mRNA, complete cds. 91 G484 gi18481628 6.70E−65 Glycine max repressor protein. 91 G484 gi18481620 4.80E−60 Oryza sativa repressor protein. 91 G484 gi18481622 2.00E−58 Triticum aestivum repressor protein. 91 G484 gi20160792 2.90E−16 Oryza sativa (japonica putative CAAT-box cultivar-group) 91 G484 gi15321716 1.30E−15 Zea mays leafy cotyledon1. 91 G484 gi22536010 1.10E−14 Phaseolus coccineus LEC1-like protein. 91 G484 gi16902054 1.50E−14 Vernonia galamensis CCAAT-box binding factor HAP3 B domai 91 G484 gi16902056 2.70E−13 Argemone mexicana CCAAT-box binding factor HAP3 B domain. 91 G484 gi18129292 1 Pinus pinaster histone H2B protein. 91 G484 gi1083950 1 Canavalia lineata subtilisin inhibitor CLSI-I- Canavalia 93 G489 BH679015 1.00E−111 Brassica oleracea BOHXO96TF BO_2_3_KB Brassica oleracea gen 93 G489 AC136503 1.00E−75 Medicago truncatula clone mth2-15n1, WORKING DRAFT SEQUENCE 93 G489 BQ118033 8.00E−73 Solanum tuberosum EST603609 mixed potato tissues Solanum tu 93 G489 BU873518 4.00E−68 Populus balsamifera Q056D09 Populus flow subsp. trichocarpa 93 G489 BI934205 2.00E−67 Lycopersicon EST554094 tomato flower, esculentum anthesis L 93 G489 BQ797616 1.00E−66 Vitis vinifera EST 6554 Ripening Grape berries Lambda Zap I 93 G489 BM064398 4.00E−63 Capsicum annuum KS01066E11 KS01 Capsicum annuum cDNA, mRNA 93 G489 BU927107 4.00E−60 Glycine max sas95f12.y1 Gm-c1036 Glycine max cDNA clone SOY 93 G489 BQ993879 6.00E−59 Lactuca sativa QGF5L12.yg.ab1 QG_EFGHJ lettuce serriola Lac 93 G489 AP004113 1.00E−58 Oryza sativa chromosome 2 clone OJ1116_A06, *** SEQUENCING 93 G489 gi5257260 6.20E−46 Oryza sativa Similar to sequence of BAC F7G19 from Arabid 93 G489 gi20804442 6.60E−19 Oryza sativa (japonica hypothetical prote cultivar-group) 93 G489 gi18481626 3.90E−09 Lea mays repressor protein. 93 G489 gi1808688 0.051 Sporobolus stapfianus hypothetical protein. 93 G489 gi8096192 0.21 Lilium longiflorum gH2A.1. 93 G489 gi2130105 0.25 Triticum aestivum histone H2A.4-wheat. 93 G489 gi297871 0.27 Picea abies histone H2A. 93 G489 gi297887 0.31 Daucus carota glycine rich protein. 93 G489 gi15214035 0.75 Cicer arietinum HISTONE H2A. 93 G489 gi2317760 0.75 Pinus taeda H2A homolog. 95 G490 AX180963 1.00E−19 Physcomitrella patens Sequence 14 from Patent WO0145493. 95 G490 AP004836 1.00E−19 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 95 G490 AU197697 1.00E−19 Oryza sativa AU197697 Rice mature leaf Oryza sativa cDNA cl 95 G490 BJ193952 1.00E−19 Physcomitrella patens BJ193952 normalized ful subsp. patens 95 G490 AAAA01011976 1.00E−19 Oryza sativa (indica ( ) scaffold011976 cultivar-group) 95 G490 BM065544 2.00E−19 Capsicum annuum KS07004F12 KS07 Capsicum annuum cDNA, mRNA 95 G490 AL749991 2.00E−19 Pinus pinaster AL749991 AS Pinus pinaster cDNA clone AS03E0 95 G490 BG440805 3.00E−19 Gossypium arboreum GA_Ea0010D12f Gossypium arboreum 7-10 d 95 G490 BE460012 4.00E−19 Lycopersicon EST415304 tomato esculentum developing/immatur 95 G490 BJ269516 4.00E−19 Triticum aestivum BJ269516 Y. Ogihara unpublished cDNA libr 95 G490 gi5257260 7.50E−18 Oryza sativa Similar to sequence of BAC F7G19 from Arabid 95 G490 gi22138475 4.00E−13 Oryza sativa (japonica putative transcrip cultivar-group) 95 G490 gi18481626 7.00E−06 Zea mays repressor protein. 95 G490 gi16902058 0.99 Triticum aestivum CCAAT-box binding factor HAP3 B domain. 95 G490 gi16902056 1 Argemone mexicana CCAAT-box binding factor HAP3 B domain. 95 G490 gi16902050 1 Glycine max CCAAT-box binding factor HAP3 B domain. 95 G490 gi16902054 1 Vernonia galamensis CCAAT-box binding factor HAP3 B domai 97 G504 BU895066 1.00E−82 Populus tremula x X018H04 Populus wood Populus tremuloides 97 G504 BI422750 2.00E−80 Lycopersicon EST533416 tomato callus, esculentum TAMU Lycop 97 G504 AW560823 5.00E−71 Medicago truncatula EST315871 DSIR Medicago truncatula cDNA 97 G504 CA815703 1.00E−68 Vitis vinifera CA12EI204IVF_E10 Cabernet Sauvignon Leaf-C 97 G504 BQ121923 2.00E−67 Solanum tuberosum EST607499 mixed potato tissues Solanum tu 97 G504 BM092513 2.00E−66 Glycine max sah14g06.y3 Gm-c1086 Glycine max cDNA clone GEN 97 G504 BI246023 4.00E−66 Sorghum bicolor IP1_66_F11.b1_A002 Immature pannicle 1 (IP1 97 G504 BU041353 1.00E−63 Prunus persica PP_LEa0009B03f Peach developing fruit mesoca 97 G504 BU672229 2.00E−63 Triticum aestivum WHE3302_A10_A20ZS Chinese Spring wheat dr 97 G504 AF402603 4.00E−62 Phaseolus vulgaris NAC domain protein NAC2 mRNA, complete c 97 G504 gi24417196 4.20E−72 Oryza sativa (japonica contains ESTs C993 cultivar-group) 97 G504 gi15148914 2.70E−61 Phaseolus vulgaris NAC domain protein NAC2. 97 G504 gi15528779 3.50E−59 Oryza sativa development regulation gene OsNAC4. 97 G504 gi6175246 2.50E−58 Lycopersicon jasmonic acid 2. esculentum 97 G504 gi21105748 4.10E−58 Petunia × hybrida nam-like protein 10. 97 G504 gi14485513 1.60E−56 Solanum tuberosum putative NAC domain protein. 97 G504 gi4218535 2.10E−54 Triticum sp. GRAB1 protein. 97 G504 gi6732158 2.10E−54 Triticum monococcum unnamed protein product. 97 G504 gi22597158 2.90E−50 Glycine max no apical meristem-like protein. 97 G504 gi7716952 2.20E−34 Medicago truncatula NAC1. 99 G509 BG646875 2.00E−68 Medicago truncatula EST508494 HOGA Medicago truncatula cDNA 99 G509 BQ850404 2.00E−65 Lactuca sativa QGB12I10.yg.ab1 QG_ABCDI lettuce salinas Lac 99 G509 BE363054 3.00E−59 Sorghum bicolor DG1_9_D04.b1_A002 Dark Grown 1 (DG1) Sorghu 99 G509 BE434322 1.00E−56 Lycopersicon EST405400 tomato breaker esculentum fruit, TIG 99 G509 BM112823 8.00E−50 Solanum tuberosum EST560359 potato roots Solanum tuberosum 99 G509 AF402602 3.00E−49 Phaseolus vulgaris NAC domain protein NAC1 mRNA, complete c 99 G509 PHRNANAM 2.00E−48 Petunia x hybrida P. hybrida mRNA encoding NAM protein. 99 G509 BZ034968 4.00E−48 Brassica oleracea oem78a04.b1 B. oleracea002 Brassica olerac 99 G509 AV923588 3.00E−46 Hordeum vulgare subsp. AV923588 K. Sato vulgare unpublished 99 G509 BE586058 4.00E−46 Triticum aestivum Est#8pT7_C09_c9_066 KSU wheat Fusarium gr 99 G509 gi13129497 6.00E−57 Oryza sativa putative NAM (no apical meristem) protein. 99 G509 gi15148912 4.80E−50 Phaseolus vulgaris NAC domain protein NAC1. 99 G509 gi24476048 3.30E−47 Oryza sativa (japonica Putative NAM (no a cultivar-group) 99 G509 gi1279640 5.40E−47 Petunia x hybrida NAM. 99 G509 gi4218537 8.50E−42 Triticum sp. GRAB2 protein. 99 G509 gi6732156 8.50E−42 Triticum monococcum unnamed protein product. 99 G509 gi22597158 1.40E−41 Glycine max no apical meristem-like protein. 99 G509 gi14485513 1.90E−37 Solanum tuberosum putative NAC domain protein. 99 G509 gi6175246 8.40E−35 Lycopersicon jasmonic acid 2. esculentum 99 G509 gi7716952 4.30E−32 Medicago truncatula NAC1. 101 G519 BG543276 9.00E−93 Brassica rapa subsp. E0770 Chinese cabbage pekinensis etiol 101 G519 BQ165234 2.00E−88 Medicago truncatula EST611103 KVKC Medicago truncatula cDNA 101 G519 AF509866 4.00E−85 Petunia x hybrida nam-like protein 3 (NH3) mRNA, complete c 101 G519 STU401151 9.00E−85 Solanum tuberosum mRNA for putative NAC domain protein (na 101 G519 BH476033 1.00E−80 Brassica oleracea BOHNV27TF BOHN Brassica oleracea genomic 101 G519 CA820578 2.00E−80 Glycine max sau91c12.y1 Gm-c1048 Glycine max cDNA clone SOY 101 G519 BM411425 1.00E−79 Lycopersicon EST585752 tomato breaker esculentum fruit Lyco 101 G519 BQ970677 1.00E−78 Helianthus annuus QHB42M12.yg.ab1 QH_ABCDI sunflower RHA801 101 G519 AB028185 2.00E−78 Oryza sativa mRNA for OsNAC6 protein, complete cds. 101 G519 BG441329 6.00E−78 Gossypium arboreum GA_Ea0012N05f Gossypium arboreum 7-10 d 101 G519 gi14485513 2.20E−86 Solanum tuberosum putative NAC domain protein. 101 G519 gi21105734 2.80E−86 Petunia x hybrida nam-like protein 3. 101 G519 gi13272281 1.40E−80 Oryza sativa NAC6. 101 G519 gi20161457 1.40E−80 Oryza sativa (japonica OsNAC6 protein. cultivar-group) 101 G519 gi4218535 1.40E−62 Triticum sp. GRAB1 protein. 101 G519 gi6732158 1.40E−62 Triticum monococcum unnamed protein product. 101 G519 gi6175246 1.30E−54 Lycopersicon jasmonic acid 2. esculentum 101 G519 gi15148914 4.30E−54 Phaseolus vulgaris NAC domain protein NAC2. 101 G519 gi22597158 1.70E−43 Glycine max no apical meristem-like protein. 101 G519 gi7716952 1.50E−35 Medicago truncatula NAC1. 103 G545 BH552655 9.00E−96 Brassica oleracea BOGEH82TF BOGE Brassica oleracea genomic 103 G545 BQ704580 7.00E−74 Brassica napus Bn01_02p11_A 103 G545 AF119050 5.00E−59 Datisca glomerata zinc-finger protein 1 (zfp1) mRNA, comple 103 G545 AP004523 9.00E−58 Lotus japonicus genomic DNA, chromosome 1, clone: LjT03J05, 103 G545 PETZFP4 2.00E−56 Petunia x hybrida Petunia zinc-finger protein gene. 103 G545 CA801331 4.00E−55 Glycine max sau04c04.y2 Gm-c1062 Glycine max cDNA clone SOY 103 G545 MSY18788 1.00E−53 Medicago sativa mRNA for putative TFIIIA (or kruppel)-like 103 G545 BG582865 2.00E−53 Medicago truncatula EST484611 GVN Medicago truncatula cDNA 103 G545 BM437679 8.00E−51 Vitis vinifera VVA023E03_54853 An expressed sequence tag da 103 G545 AF053077 8.00E−49 Nicotiana tabacum osmotic stress-induced zinc- finger protei 103 G545 gi4666360 6.00E−57 Datisca glomerata zinc-finger protein 1. 103 G545 gi7228329 2.70E−54 Medicago sativa putative TFIIIA (or kruppel)-like zinc fi 103 G545 gi1763063 9.00E−54 Glycine max SCOF-1. 103 G545 gi439487 4.70E−44 Petunia x hybrida zinc-finger DNA binding protein. 103 G545 gi2058504 1.50E−35 Brassica rapa zinc-finger protein-1. 103 G545 gi2981169 4.30E−31 Nicotiana tabacum osmotic stress-induced zinc- finger prot 103 G545 gi485814 6.50E−28 Triticum aestivum WZF1. 103 G545 gi12698882 2.90E−25 Oryza sativa zinc finger transcription factor ZF1. 103 G545 gi21104613 1.90E−14 Oryza sativa (japonica contains ESTs AU07 cultivar-group) 103 G545 gi2129892 4.70E−06 Pisum sativum probable finger protein Pszf1-garden pea. 105 G546 BG544345 3.00E−61 Brassica rapa subsp. E2200 Chinese cabbage pekinensis etiol 105 G546 BH424854 6.00E−49 Brassica oleracea BOGML16TF BOGM Brassica oleracea genomic 105 G546 AW223952 2.00E−45 Lycopersicon EST300763 tomato fruit red esculentum ripe, TA 105 G546 BG889076 4.00E−45 Solanum tuberosum EST514927 cSTD Solanum tuberosum cDNA clo 105 G546 AC127019 3.00E−44 Medicago truncatula clone mth2-31b1, WORKING DRAFT SEQUENCE 105 G546 BF597949 9.00E−42 Glycine max su89e06.y1 Gm-c1055 Glycine max cDNA clone GENO 105 G546 BE033932 2.00E−40 Mesembryanthemum MG02C06 MG crystallinum Mesembryanthemum c 105 G546 OSJN00157 3.00E−37 Oryza sativa chromosome 4 clone OSJNBa0013K16, *** SEQUENC 105 G546 BI418846 3.00E−37 Lotus japonicus LjNEST36e5r Lotus japonicus nodule library 105 G546 AAAA01035793 3.00E−37 Oryza sativa (indica ( ) scaffold035793 cultivar-group) 105 G546 gi2894379 3.10E−37 Hordeum vulgare ring finger protein. 105 G546 gi12039329 9.00E−34 Oryza sativa putative ring finger protein. 105 G546 gi19571069 1.80E−25 Oryza sativa (japonica contains EST C7268 cultivar-group) 105 G546 gi17016985 3.00E−23 Cucumis melo RING-H2 zinc finger protein. 105 G546 gi21645888 5.90E−18 Zea mays ring-H2 zinc finger protein. 105 G546 gi23451086 2.10E−14 Medicago sativa RING-H2 protein. 105 G546 gi12003386 6.30E−14 Nicotiana tabacum Avr9/Cf-9 rapidly elicited protein 132. 105 G546 gi20152976 4.00E−12 Hordeum vulgare subsp. similar to A. thaliana C3H vulgare 105 G546 gi22597166 8.70E−12 Glycine max RING-H2 finger protein. 105 G546 gi1086225 3.50E−09 Lotus japonicus RING-finger protein-Lotus japonicus. 107 G561 SAY16953 1.00E−146 Sinapis alba mRNA for G-box binding factor 2A. 107 G561 BNGBBF2A 1.00E−141 Brassica napus B. napus mRNA for G-Box binding factor 2A. 107 G561 RSGBOX 1.00E−141 Raphanus sativus R. sativus mRNA for G-box binding protein. 107 G561 PVU41817 8.00E−78 Phaseolus vulgaris regulator of MAT2 (ROM2) mRNA, complete 107 G561 AF084971 7.00E−77 Catharanthus roseus G-box binding protein 1 (GBF1) mRNA, co 107 G561 SOAJ3624 2.00E−75 Spinacia oleracea mRNA for basic leucine zipper protein. 107 G561 SOYGBFB 1.00E−72 Glycine max G-box binding factor (GBF2A) mRNA, 3′ end. 107 G561 NTTAF2MR 2.00E−70 Nicotiana tabacum N. tabacum mRNA for TAF- 2. 107 G561 PCCPRF1 5.00E−66 Petroselinum crispum P. crispum CPRF1 mRNA for light-inducib 107 G561 ZMU10270 6.00E−49 Zea mays G-box binding factor 1 (GBF1) mRNA, complete cds. 107 G561 gi2995462 1.00E−139 Sinapis alba G-box binding protein. 107 G561 gi1076448 2.30E−135 Brassica napus G-box binding factor 2A- rape. 107 G561 gi1033059 4.80E−135 Raphanus sativus G-Box binding protein. 107 G561 gi1155054 2.30E−58 Phaseolus vulgaris regulator of MAT2. 107 G561 gi5381311 3.50E−52 Catharanthus roseus G-box binding protein 1. 107 G561 gi2815305 4.00E−51 Spinacia oleracea basic leucine zipper protein. 107 G561 gi169959 1.20E−49 Glycine max G-box binding factor. 107 G561 gi1076623 8.00E−46 Nicotiana tabacum G-box-binding protein TAF- 2-common to 107 G561 gi498643 1.30E−45 Zea mays G-box binding factor 1. 107 G561 gi100162 5.20E−42 Petroselinum crispum light-induced protein CPRF- 1-parsl 109 G562 BNU27108 1.00E−160 Brassica napus transcription factor (BnGBF1) mRNA, partial 109 G562 AF084971 1.00E−102 Catharanthus roseus G-box binding protein 1 (GBF1) mRNA, co 109 G562 PVU41817 1.00E−96 Phaseolus vulgaris regulator of MAT2 (ROM2) mRNA, complete 109 G562 SOYGBFB 2.00E−94 Glycine max G-box binding factor (GBF2A) mRNA, 3′ end. 109 G562 SOAJ3624 9.00E−94 Spinacia oleracea mRNA for basic leucine zipper protein. 109 G562 NTTAF2MR 4.00E−89 Nicotiana tabacum N. tabacum mRNA for TAF- 2. 109 G562 PCCPRF1 1.00E−84 Petroselinum crispum P. crispum CPRF1 mRNA for light-inducib 109 G562 SAY16953 2.00E−81 Sinapis alba mRNA for G-box binding factor 2A. 109 G562 RSGBOX 6.00E−79 Raphanus sativus R. sativus mRNA for G-box binding protein. 109 G562 BF271790 6.00E−58 Gossypium arboreum GA_Eb0012L24f Gossypium arboreum 7-10 d 109 G562 gi1399005 2.00E−159 Brassica napus transcription factor. 109 G562 gi2995462 6.70E−81 Sinapis alba G-box binding protein. 109 G562 gi1033059 1.80E−78 Raphanus sativus G-Box binding protein. 109 G562 gi5381311 1.20E−60 Catharanthus roseus G-box binding protein 1. 109 G562 gi2815305 1.20E−60 Spinacia oleracea basic leucine zipper protein. 109 G562 gi1169081 2.20E−59 Petroselinum crispum COMMON PLANT REGULATORY FACTOR CPRF- 109 G562 gi169959 5.40E−56 Glycine max G-box binding factor. 109 G562 gi1155054 1.80E−55 Phaseolus vulgaris regulator of MAT2. 109 G562 gi498643 2.10E−52 Zea mays G-box binding factor 1. 109 G562 gi1076624 1.30E−47 Nicotiana tabacum G-box-binding protein TAF- 3-common to 111 G567 PCCPRF2 1.00E−55 Petroselinum crispum P. crispum CPRF2 mRNA for DNA-binding p 111 G567 AY061648 8.00E−53 Nicotiana tabacum bZIP transcription factor (BZI-1) mRNA, c 111 G567 BH590739 2.00E−48 Brassica oleracea BOHCB55TR BOHC Brassica oleracea genomic 111 G567 GMGHBF1 2.00E−47 Glycine max G. max mRNA for G/HBF-1. 111 G567 RICBZIPPA 2.00E−44 Oryza sativa mRNA for bZIP protein, complete cds. 111 G567 MZEBZIP 2.00E−43 Zea mays opaque2 heterodimerizing protein 2 mRNA, complete 111 G567 BU041142 3.00E−43 Prunus persica PP_LEa0008G18f Peach developing fruit mesoca 111 G567 BG645542 4.00E−42 Medicago truncatula EST507161 KV3 Medicago truncatula cDNA 111 G567 AJ487392 4.00E−41 Solanum tuberosum AJ487392 Solanum tuberosum cv. Provita So 111 G567 AW647973 9.00E−41 Lycopersicon EST326427 tomato esculentum germinating seedli 111 G567 gi1806261 1.60E−49 Petroselinum crispum DNA-binding protein; bZIP type. 111 G567 gi1783305 1.80E−46 Oryza sativa bZIP protein. 111 G567 gi16797791 8.20E−44 Nicotiana tabacum bZIP transcription factor. 111 G567 gi168428 8.20E−44 Zea mays opaque2 heterodimerizing protein 2. 111 G567 gi1905785 2.20E−43 Glycine max G/HBF-1. 111 G567 gi1869928 9.70E−41 Hordeum vulgare blz-1 protein. 111 G567 gi463212 4.40E−34 Coix lacryma-jobi opaque 2. 111 G567 gi1362178 1.00E−32 Sorghum bicolor opaque-2 protein-sorghum. 111 G567 gi21435101 2.90E−32 Pennisetum glaucum opaque-2-like protein. 111 G567 gi1654099 2.30E−24 Triticum aestivum transcriptional activator. 113 G568 BH994972 1.00E−64 Brassica oleracea oeh20b03.b1 B. oleracea002 Brassica olerac 113 G568 AF288616 2.00E−42 Populus balsamifera subsp. trichocarpa x Populus deltoides 113 G568 BU834855 1.00E−25 Populus tremula x T066E09 Populus apica Populus tremuloides 113 G568 BU819252 5.00E−23 Populus tremula UA41BPE07 Populus tremula cambium cDNA libr 113 G568 AC123571 7.00E−17 Medicago truncatula clone mth1-14n3, WORKING DRAFT SEQUENCE 113 G568 AV914686 8.00E−14 Hordeum vulgare subsp. AV914686 K. Sato vulgare unpublished 113 G568 AF001454 8.00E−14 Helianthus annuus Dc3 promoter-binding factor-2 (DPBF-2) mR 113 G568 BE657320 1.00E−13 Glycine max GM700001A20B6 Gm- r1070 Glycine max cDNA clone G 113 G568 CA765468 2.00E−13 Oryza sativa (indica AF53-Rpf_07_J23_T7_086 cultivar-group) 113 G568 AL819191 2.00E−13 Triticum aestivum AL819191 n: 129 Triticum aestivum cDNA clo 113 G568 gi13435335 4.20E−47 Populus x generosa basic leucine zipper transcription fac 113 G568 gi22324425 6.30E−23 Oryza sativa (japonica bZIP transcription cultivar-group) 113 G568 gi2228773 3.30E−17 Helianthus annuus Dc3 promoter-binding factor-2. 113 G568 gi21693583 8.70E−15 Triticum aestivum ABA response element binding factor. 113 G568 gi5821255 4.90E−13 Oryza sativa TRAB1. 113 G568 gi13775111 4.20E−12 Phaseolus vulgaris bZIP transcription factor 6. 113 G568 gi7406677 3.30E−11 Vitis vinifera putative ripening-related bZIP protein. 113 G568 gi14571808 2.90E−10 Nicotiana tabacum phi-2. 113 G568 gi6018699 3.10E−10 Lycopersicon THY5 protein. esculentum 113 G568 gi1352613 3.20E−10 Zea mays OCS-ELEMENT BINDING FACTOR 1 (OCSBF-1). 115 G584 PVU18348 1.00E−166 Phaseolus vulgaris phaseolin G-box binding protein PG1 (PG1 115 G584 BH696428 5.00E−94 Brassica oleracea BOMCR67TF BO_2_3_KB Brassica oleracea gen 115 G584 AF011557 7.00E−80 Lycopersicon jasmonic acid 3 (LEJA3) esculentum mRNA, parti 115 G584 BI434651 9.00E−75 Solanum tuberosum EST537412 P. infestans- challenged leaf So 115 G584 AF061107 2.00E−70 Zea mays transcription factor MYC7E mRNA, partial cds. 115 G584 BG453241 3.00E−70 Medicago truncatula NF090G06LF1F1049 Developing leaf Medica 115 G584 AAAA01004195 2.00E−68 Oryza sativa (indica ( ) scaffold004195 cultivar-group) 115 G584 AC060755 6.00E−68 Oryza sativa chromosome 10 clone OSJNBa0003O19, *** SEQUENC 115 G584 BG446831 7.00E−67 Gossypium arboreum GA_Eb0039H18f Gossypium arboreum 7-10 d 115 G584 BI968400 2.00E−62 Glycine max GM830005A12E12 Gm- r1083 Glycine max cDNA clone 115 G584 gi1142619 3.90E−155 Phaseolus vulgaris phaseolin G-box binding protein PG1. 115 G584 gi12643064 1.00E−131 Oryza sativa putative MYC transcription factor. 115 G584 gi4321762 4.30E−130 Zea mays transcription factor MYC7E. 115 G584 gi6175252 2.30E−62 Lycopersicon jasmonic acid 3. esculentum 115 G584 gi19571087 2.70E−47 Oryza sativa (japonica contains EST AU031 cultivar-group) 115 G584 gi10998404 1.40E−37 Petunia x hybrida anthocyanin 1. 115 G584 gi4519201 9.30E−30 Perilla frutescens MYC-GP. 115 G584 gi166428 8.00E−28 Antirrhinum majus DEL. 115 G584 gi13346182 3.00E−27 Gossypium hirsutum GHDEL65. 115 G584 gi3650292 5.10E−18 Gerbera hybrida GMYC1 protein. 117 G585 AF336280 1.00E−165 Gossypium hirsutum GHDEL65 (ghdel65) mRNA, complete cds. 117 G585 AMADEL 1.00E−147 Antirrhinum majus DEL (delila) mRNA, complete cds. 117 G585 AB024050 1.00E−142 Perilla frutescens mRNA for MYC-RP, complete cds. 117 G585 AF020545 1.00E−135 Petunia x hybrida bHLH transcription factor JAF13 (jaf13) m 117 G585 GHY7709 1.00E−107 Gerbera hybrida mRNA for bHLH transcription factor. 117 G585 AX540498 1.00E−104 Lotus uliginosus Sequence 2 from Patent WO0210412. 117 G585 ZMA251719 9.00E−81 Zea mays mRNA for transcription factor (hopi gene). 117 G585 AF503363 3.00E−67 Lotus japonicus myc-like regulatory protein (TAN1) mRNA, pa 117 G585 BI308638 7.00E−67 Medicago truncatula EST530048 GPOD Medicago truncatula cDNA 117 G585 BU875274 1.00E−57 Populus balsamifera V004CE04 Populus flo subsp. trichocarpa 117 G585 gi13346182 6.30E−156 Gossypium hirsutum GHDEL65. 117 G585 gi166428 5.70E−139 Antirrhinum majus DEL. 117 G585 gi4519199 2.60E−127 Perilla frutescens MYC-RP. 117 G585 gi3127045 5.40E−127 Petunia x hybrida bHLH transcription factor JAF13. 117 G585 gi3650292 1.30E−93 Gerbera hybrida GMYC1 protein. 117 G585 gi8052457 2.00E−87 Zea mays transcription factor. 117 G585 gi1086540 2.20E−86 Oryza sativa Ra. 117 G585 gi20467247 2.40E−83 Lotus uliginosus myc-like regulatory protein. 117 G585 gi20467249 5.90E−66 Lotus japonicus myc-like regulatory protein. 117 G585 gi21429235 1.70E−50 Onobrychis viciifolia basic helix-loop-helix regulatory p 119 G590 AW782148 1.00E−49 Glycine max sm02b05.y1 Gm-c1027 Glycine max cDNA clone GENO 119 G590 AW649972 5.00E−45 Lycopersicon EST328426 tomato esculentum germinating seedli 119 G590 BZ045178 2.00E−37 Brassica oleracea 1kf53d05.g1 B. oleracea002 Brassica olerac 119 G590 BM408345 3.00E−31 Solanum tuberosum EST582672 potato roots Solanum tuberosum 119 G590 BM065639 4.00E−31 Capsicum annuum KS07005G09 KS07 Capsicum annuum cDNA, mRNA 119 G590 BI308330 1.00E−30 Medicago truncatula EST529740 GPOD Medicago truncatula cDNA 119 G590 BQ134415 5.00E−28 Zea mays 1091016H12.y2 1091- Immature ear with common ESTs 119 G590 BU866069 1.00E−25 Populus tremula x S062C11 Populus imbib Populus tremuloides 119 G590 AU290290 1.00E−24 Zinnia elegans AU290290 zinnia cultured mesophyll cell equa 119 G590 BU574318 1.00E−24 Prunus dulcis PA_Ea0007A10f Almond developing seed Prunus 119 G590 gi15451582 7.80E−32 Oryza sativa Putative SPATULA. 119 G590 gi23495742 8.20E−28 Oryza sativa (japonica putative phytochro cultivar-group) 119 G590 gi5923912 5.40E−10 Tulipa gesneriana bHLH transcription factor GBOF-1. 119 G590 gi527657 1.40E−09 Pennisetum glaucum myc-like regulatory R gene product. 119 G590 gi6166283 2.30E−09 Pinus taeda helix-loop-helix protein 1A. 119 G590 gi527665 4.80E−09 Sorghum bicolor myc-like regulatory R gene product. 119 G590 gi527661 1.00E−08 Phyllostachys acuta myc-like regulatory R gene product. 119 G590 gi1086534 1.70E−08 Oryza officinalis transcriptional activator Ra homolog. 119 G590 gi1086526 2.80E−08 Oryza australiensis transcriptional activator Ra homolog. 119 G590 gi1086538 4.60E−08 Oryza rufipogon transcriptional activator Rb homolog. 121 G594 BE807866 4.00E−38 Glycine max ss31c06.y1 Gm-c1061 Glycine max cDNA clone GENO 121 G594 BQ875608 5.00E−38 Lactuca sativa QGI8J14.yg.ab1 QG_ABCDI lettuce salinas Lact 121 G594 BU791131 1.00E−36 Populus balsamifera subsp. trichocarpa x Populus deltoides 121 G594 CA015610 9.00E−35 Hordeum vulgare subsp. HT14N12r HT Hordeum vulgare vulgare 121 G594 BF200249 2.00E−34 Triticum monococcum WHE2254_F11_L22ZE Triticum monococcum s 121 G594 BM497415 6.00E−34 Avicennia marina 901269 Avicennia marina leaf cDNA Library 121 G594 AW906522 4.00E−33 Solanum tuberosum EST342644 potato stolon, Cornell Universi 121 G594 AI731417 5.00E−33 Gossypium hirsutum BNLGHi9478 Six-day Cotton fiber Gossypiu 121 G594 BE455695 5.00E−33 Hordeum vulgare HVSMEg0019A10f Hordeum vulgare pre- anthesis 121 G594 BE360329 5.00E−33 Sorghum bicolor DG1_62_C04.g1_A002 Dark Grown 1 (DG1) Sorgh 121 G594 gi20804997 2.20E−34 Oryza sativa (japonica DNA-binding protei cultivar-group) 121 G594 gi11862964 6.00E−34 Oryza sativa hypothetical protein. 121 G594 gi5923912 3.40E−31 Tulipa gesneriana bHLH transcription factor GBOF-1. 121 G594 gi6166283 4.30E−10 Pinus taeda helix-loop-helix protein 1A. 121 G594 gi13346182 3.80E−06 Gossypium hirsutum GHDEL65. 121 G594 gi527665 4.80E−06 Sorghum bicolor myc-like regulatory R gene product. 121 G594 gi527661 6.20E−06 Phyllostachys acuta myc-like regulatory R gene product. 121 G594 gi4206118 6.60E−06 Mesembryanthemum transporter homolog. crystallinum 121 G594 gi527657 1.30E−05 Pennisetum glaucum myc-like regulatory R gene product. 121 G594 gi1086526 0.0001 Oryza australiensis transcriptional activator Ra homolog. 123 G597 BE600816 5.00E−62 Sorghum bicolor PI1_90_E07.b1_A002 Pathogen induced 1 (PI1) 123 G597 AY106980 3.00E−60 Zea mays PCO106555 mRNA sequence. 123 G597 BQ765321 3.00E−58 Hordeum vulgare EBro03_SQ006_H21_R root, 3 week, waterlogge 123 G597 CA501339 2.00E−57 Triticum aestivum WHE4032_D07_H14ZT Wheat meiotic anther cD 123 G597 BQ841090 1.00E−56 Aegilops speltoides WHE4206_H10_O20ZS Aegilops speltoides p 123 G597 BG465540 8.00E−56 Sorghum propinquum RHIZ2_45_G09.b1_A003 Rhizome2 (RHIZ2) So 123 G597 AW928863 7.00E−53 Lycopersicon EST337651 tomato flower esculentum buds 8 mm t 123 G597 BQ856774 4.00E−51 Lactuca sativa QGB5L17.yg.ab1 QG_ABCDI lettuce salinas Lact 123 G597 BU926769 5.00E−51 Glycine max sas91d09.y1 Gm-c1036 Glycine max cDNA clone SOY 123 G597 BJ473026 1.00E−50 Hordeum vulgare subsp. BJ473026 K. Sato vulgare unpublished 123 G597 gi12643044 1.60E−65 Oryza sativa putative AT-Hook DNA- binding protein. 123 G597 gi2213536 3.20E−49 Pisum sativum DNA-binding protein PD1. 123 G597 gi4165183 2.90E−41 Antirrhinum majus SAP1 protein. 123 G597 gi24418033 4.20E−15 Oryza sativa (japonica Hypothetical prote cultivar-group) 123 G597 gi13992574 0.00058 Triticum timopheevii glutenin HMW subunit 1Ax. 123 G597 gi100787 0.0011 Triticum aestivum glutenin high molecular weight chain 1A 123 G597 gi7188720 0.0032 Aegilops ventricosa x-type high molecular weight glutenin 123 G597 gi456124 0.066 Nicotiana tabacum DNA-binding protein. 123 G597 gi21218057 0.076 Chlamydomonas putative Pi-transporter reinhardtii homolog 123 G597 gi21779920 0.14 Aegilops tauschii HMW-glutenin. 125 G598 BH488116 9.00E−41 Brassica oleracea BOHPM37TF BOHP Brassica oleracea genomic 125 G598 BG455043 9.00E−38 Medicago truncatula NF112G09LF1F1069 Developing leaf Medica 125 G598 BQ856793 3.00E−35 Lactuca sativa QGB5M13.yg.ab1 QG_ABCDI lettuce salinas Lact 125 G598 AW932217 3.00E−33 Lycopersicon EST358060 tomato fruit esculentum mature green 125 G598 BQ511117 5.00E−31 Solanum tuberosum EST618532 Generation of a set of potato c 125 G598 AP003981 3.00E−30 Oryza sativa chromosome 7 clone OJ1019_E02, *** SEQUENCING 125 G598 AAAA01001857 3.00E−30 Oryza sativa (indica ( ) scaffold001857 cultivar-group) 125 G598 AC135958 7.00E−30 Oryza sativa (japonica ( ) chromosome 3 clo cultivar-group) 125 G598 BG319716 9.00E−23 Zea mays Zm03_06a07_A Zm03_AAFC_ECORC_cold_stressed_maize_s 125 G598 BU025013 2.00E−20 Helianthus annuus QHF7D11.yg.ab1 QH_EFGHJ sunflower RHA280 125 G598 gi1881585 0.059 Solanum tuberosum remorin. 125 G598 gi15289949 0.11 Oryza sativa (japonica hypothetical prote cultivar-group) 125 G598 gi4883530 0.32 Lycopersicon remorin 2. esculentum 125 G598 gi13161367 0.96 Oryza sativa hypothetical protein. 125 G598 gi13775109 0.97 Phaseolus vulgaris bZIP transcription factor 3. 125 G598 gi8096269 0.98 Nicotiana tabacum KED. 125 G598 gi2598161 0.98 Pinus strobus NADPH: protochlorophyllide oxidoreductase po 125 G598 gi1183880 0.99 Brassica napus oleosin-like protein. 125 G598 gi22002966 1 Hordeum vulgare subsp. putative CENP-E like kinet vulgare 125 G598 gi4185307 1 Zea mays unknown. 127 G634 OSGT2 2.00E−47 Oryza sativa O. sativa gt-2 gene. 127 G634 BU049946 1.00E−46 Zea mays 1111017E09.y1 1111- Unigene III from Maize Genome 127 G634 AF372499 6.00E−38 Glycine max GT-2 factor mRNA, partial cds. 127 G634 AB052729 4.00E−37 Pisum sativum mRNA for DNA-binding protein DF1, complete cd 127 G634 BU889446 4.00E−36 Populus tremula P021A05 Populus petioles cDNA library Popul 127 G634 BH436958 2.00E−35 Brassica oleracea BOHBE67TF BOHB Brassica oleracea genomic 127 G634 AI777252 3.00E−35 Lycopersicon EST258217 tomato esculentum resistant, Cornell 127 G634 AW686754 1.00E−33 Medicago truncatula NF042C08NR1F1000 Nodulated root Medicag 127 G634 AV410715 4.00E−33 Lotus japonicus AV410715 Lotus japonicus young plants (two- 127 G634 AI730933 8.00E−30 Gossypium hirsutum BNLGHi8208 Six-day Cotton fiber Gossypiu 127 G634 gi13786451 3.20E−78 Oryza sativa putative transcription factor. 127 G634 gi13646986 3.50E−66 Pisum sativum DNA-binding protein DF1. 127 G634 gi18182311 2.70E−38 Glycine max GT-2 factor. 127 G634 gi20161567 8.90E−11 Oryza sativa (japonica hypothetical prote cultivar-group) 127 G634 gi170271 4.70E−08 Nicotiana tabacum DNA-binding protein. 127 G634 gi18349 0.0027 Daucus carota glycine rich protein (AA 1- 96). 127 G634 gi21388658 0.027 Physcomitrella patens glycine-rich RNA binding protein. 127 G634 gi21322752 0.052 Triticum aestivum cold shock protein-1. 127 G634 gi3126963 0.057 Elaeagnus umbellata acidic chitinase. 127 G634 gi1166450 0.087 Lycopersicon Tfm5. esculentum 129 G635 BH528345 1.00E−117 Brassica oleracea BOGNZ34TR BOGN Brassica oleracea genomic 129 G635 BQ916526 4.00E−71 Helianthus annuus QHB18C05.yg.ab1 QH_ABCDI sunflower RHA801 129 G635 AY110231 1.00E−68 Zea mays CL852_1 mRNA sequence. 129 G635 BI139375 3.00E−42 Populus balsamifera F130P49Y Populus flo subsp. trichocarpa 129 G635 BQ850859 3.00E−42 Lactuca sativa QGB13M04.yg.ab1 QG_ABCDI lettuce salinas Lac 129 G635 AC137603 6.00E−40 Medicago truncatula clone mth2-14b10, WORKING DRAFT SEQUENC 129 G635 BF269947 6.00E−37 Gossypium arboreum GA_Eb0006B11f Gossypium arboreum 7-10 d 129 G635 AW760602 5.00E−34 Glycine max s152e02.y1 Gm-c1027 Glycine max cDNA clone GENO 129 G635 BJ464004 1.00E−30 Hordeum vulgare subsp. BJ464004 K. Sato vulgare unpublished 129 G635 AAAA01000007 1.00E−30 Oryza sativa (indica ( ) scaffold000007 cultivar-group) 129 G635 gi21741458 3.30E−08 Oryza sativa OJ000223_09.14. 129 G635 gi170271 1.20E−07 Nicotiana tabacum DNA-binding protein. 129 G635 gi18182309 3.00E−06 Glycine max GT-2 factor. 129 G635 gi13646986 3.10E−05 Pisum sativum DNA-binding protein DF1. 129 G635 gi22128704 0.02 Oryza sativa (japonica hypothetical prote cultivar-group) 129 G635 gi7208779 0.04 Cicer arietinum hypothetical protein. 129 G635 gi1279563 0.056 Medicago sativa nuM1. 129 G635 gi15144506 0.066 Lycopersicon unknown. esculentum 129 G635 gi349585 0.36 Volvox carteri histone H1-I. 129 G635 gi2911292 0.49 Capsicum annuum prosystemin. 131 G636 AB052729 1.00E−134 Pisum sativum mRNA for DNA-binding protein DF1, complete cd 131 G636 OSGT2 1.00E−109 Oryza sativa O. sativa gt-2 gene. 131 G636 AF372498 1.00E−103 Glycine max GT-2 factor mRNA, partial cds. 131 G636 AAAA01017145 1.00E−101 Oryza sativa (indica ( ) scaffold017145 cultivar-group) 131 G636 BH521870 4.00E−89 Brassica oleracea BOGMP76TF BOGM Brassica oleracea genomic 131 G636 AP004868 2.00E−79 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 131 G636 BU894555 2.00E−69 Populus tremula x X011B09 Populus wood Populus tremuloides 131 G636 BG446849 2.00E−57 Gossypium arboreum GA_Eb0039I22f Gossypium arboreum 7-10 d 131 G636 AW032956 3.00E−52 Lycopersicon EST276515 tomato callus, esculentum TAMU Lycop 131 G636 AC135565 4.00E−49 Medicago truncatula clone mth2-19b12, WORKING DRAFT SEQUENC 131 G636 gi13646986 4.50E−111 Pisum sativum DNA-binding protein DF1. 131 G636 gi18182309 4.00E−99 Glycine max GT-2 factor. 131 G636 gi13786451 5.30E−98 Oryza sativa putative transcription factor. 131 G636 gi170271 4.30E−13 Nicotiana tabacum DNA-binding protein. 131 G636 gi20161567 4.00E−09 Oryza sativa (japonica hypothetical prote cultivar-group) 131 G636 gi10636140 0.00014 Aegilops speltoides gamma-gliadin. 131 G636 gi442524 0.00015 Hordeum vulgare C-hordein. 131 G636 gi15148391 0.00021 Triticum aestivum gamma-gliadin. 131 G636 gi225589 0.00021 Hordeum vulgare var. hordein C. distichum 131 G636 gi4584086 0.00061 Spermatozopsis similis p210 protein. 133 G638 BZ034676 3.00E−87 Brassica oleracea oef83a05.g1 B. oleracea002 Brassica olerac 133 G638 BQ866994 6.00E−55 Lactuca sativa QGC9I02.yg.ab1 QG_ABCDI lettuce salinas Lact 133 G638 BM110736 1.00E−54 Solanum tuberosum EST558272 potato roots Solanum tuberosum 133 G638 BF646615 9.00E−48 Medicago truncatula NF066C08EC1F1065 Elicited cell culture 133 G638 OSGT2 3.00E−36 Oryza sativa O. sativa gt-2 gene. 133 G638 AP004868 4.00E−33 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 133 G638 AB052729 2.00E−32 Pisum sativum mRNA for DNA-binding protein DF1, complete cd 133 G638 AI777252 4.00E−29 Lycopersicon EST258217 tomato esculentum resistant, Cornell 133 G638 BM500043 2.00E−28 Zea mays 952036C09.y1 952-BMS tissue from Walbot Lab (red 133 G638 AF372499 5.00E−28 Glycine max GT-2 factor mRNA, partial cds. 133 G638 gi20249 2.00E−49 Oryza sativa gt-2. 133 G638 gi13646986 4.30E−45 Pisum sativum DNA-binding protein DF1. 133 G638 gi18182311 1.10E−30 Glycine max GT-2 factor. 133 G638 gi20161567 2.60E−07 Oryza sativa (japonica hypothetical prote cultivar-group) 133 G638 gi170271 3.40E−06 Nicotiana tabacum DNA-binding protein. 133 G638 gi21068672 3.60E−05 Cicer arietinum putative glicine-rich protein. 133 G638 gi20257673 4.60E−05 Zea mays glycine-rich RNA binding protein. 133 G638 gi21388660 0.00014 Physcomitrella patens glycine-rich RNA-binding protein. 133 G638 gi9755844 0.00033 Brassica napus putative glycine-rich protein. 133 G638 gi1166450 0.00037 Lycopersicon Tfm5. esculentum 135 G652 BH926980 5.00E−90 Brassica oleracea odi21g11.g1 B. oleracea002 Brassica olerac 135 G652 NSGRP2MR 1.00E−71 Nicotiana sylvestris N. sylvestris mRNA for glycine rich pro 135 G652 AI812203 7.00E−65 Zea mays 605086G09.y1 605- Endosperm cDNA library from Sch 135 G652 BM408211 4.00E−64 Solanum tuberosum EST582538 potato roots Solanum tuberosum 135 G652 AP003879 6.00E−64 Oryza sativa chromosome 8 clone OJ1123_A02, *** SEQUENCING 135 G652 AP004591 6.00E−64 Oryza sativa (japonica ( ) chromosome 8 clo cultivar-group) 135 G652 AAAA01000576 7.00E−63 Oryza sativa (indica ( ) scaffold000576 cultivar-group) 135 G652 AB066265 1.00E−62 Triticum aestivum WCSP1 mRNA for cold shock protein-1, comp 135 G652 BQ840577 2.00E−62 Aegilops speltoides WHE4201_B07_C13ZS Aegilops speltoides p 135 G652 BE035242 1.00E−53 Mesembryanthemum MO03A01 MO crystallinum Mesembryanthemum c 135 G652 gi121631 9.30E−68 Nicotiana sylvestris GLYCINE-RICH CELL WALL STRUCTURAL PR 135 G652 gi21322752 1.70E−61 Triticum aestivum cold shock protein-1. 135 G652 gi121628 5.00E−26 Phaseolus vulgaris GLYCINE-RICH CELL WALL STRUCTURAL PROT 135 G652 gi395147 7.10E−25 Nicotiana tabacum glycine-rich protein. 135 G652 gi17821 1.40E−23 Brassica napus glycine-rich_protein_(aa1- 291). 135 G652 gi121627 1.80E−23 Petunia x hybrida GLYCINE-RICH CELL WALL STRUCTURAL PROTE 135 G652 gi225181 1.80E−23 Petunia sp. Gly rich structural protein. 135 G652 gi15528745 2.00E−22 Oryza sativa contains ESTs AU093876(E1018), AU093877 (E1018 135 G652 gi21327989 2.00E−22 Oryza sativa (japonica contains ESTs AU09 cultivar-group) 135 G652 gi21388660 4.40E−22 Physcomitrella patens glycine-rich RNA-binding protein. 137 G663 AF146702 6.00E−54 Petunia x hybrida An2 protein (an2) mRNA, an2-V26 allele, c 137 G663 AF146703 3.00E−53 Petunia integrifolia An2 protein (an2) mRNA, an2-S9 allele, 137 G663 BQ990780 4.00E−51 Lactuca sativa QGF21B10.yg.ab1 QG_EFGHJ lettuce serriola La 137 G663 BE462282 3.00E−50 Lycopersicon EST324546 tomato flower esculentum buds 0-3 mm 137 G663 AB073013 6.00E−50 Vitis labrusca × Vitis VlmybA2 gene for myb- vinifera relate 137 G663 AF146709 2.00E−49 Petunia axillaris An2 truncated protein (an2) mRNA, an2-S7 137 G663 BH480961 3.00E−47 Brassica oleracea BOGZT54TF BOGZ Brassica oleracea genomic 137 G663 BF635572 6.00E−42 Medicago truncatula NF104H01DT1F1014 Drought Medicago trunc 137 G663 BQ105368 2.00E−41 Rosa hybrid cultivar fc0707.e Rose Petals (Fragrant Cloud) 137 G663 AF336278 2.00E−41 Gossypium hirsutum BNLGHi233 (bnlghi6233) mRNA, complete cd 137 G663 gi7673084 1.10E−53 Petunia x hybrida An2 protein. 137 G663 gi7673086 3.90E−53 Petunia integrifolia An2 protein. 137 G663 gi22266667 2.30E−50 Vitis labrusca x Vitis myb-related transcription vinifera 137 G663 gi7673096 1.30E−47 Petunia axillaris An2 truncated protein. 137 G663 gi13346178 2.30E−41 Gossypium hirsutum BNLGHi233. 137 G663 gi1101770 8.40E−41 Picea mariana MYB-like transcriptional factor MBF1. 137 G663 gi22535556 1.20E−39 Oryza sativa (japonica myb-related protei cultivar-group) 137 G663 gi2605623 1.20E−39 Oryza sativa OSMYB4. 137 G663 gi2343273 4.80E−39 Zea mays PL transcription factor. 137 G663 gi4138299 4.80E−39 Oryza sativa subsp. transcriptional activator. indica 139 G664 AF336286 2.00E−89 Gossypium hirsutum GHMYB9 (ghmyb9) mRNA, complete cds. 139 G664 LETHM27 7.00E−88 Lycopersicon L. esculentum mRNA for esculentum THM27 protein 139 G664 BG442984 9.00E−83 Gossypium arboreum GA_Ea0019B05f Gossypium arboreum 7-10 d 139 G664 BM112753 1.00E−80 Solanum tuberosum EST560289 potato roots Solanum tuberosum 139 G664 AY108280 5.00E−78 Zea mays PCO132931 mRNA sequence. 139 G664 BF716393 2.00E−76 Glycine max saa19f01.y1 Gm-c1058 Glycine max cDNA clone GEN 139 G664 BH537477 5.00E−76 Brassica oleracea BOGIR45TF BOGI Brassica oleracea genomic 139 G664 HVMYB1 1.00E−75 Hordeum vulgare H. vulgare myb1 mRNA. 139 G664 AW775893 1.00E−74 Medicago truncatula EST334958 DSIL Medicago truncatula cDNA 139 G664 BQ855835 8.00E−73 Lactuca sativa QGB27N20.yg.ab1 QG_ABCDI lettuce salinas Lac 139 G664 gi13346194 3.50E−88 Gossypium hirsutum GHMYB9. 139 G664 gi1167484 8.00E−85 Lycopersicon transcription factor. esculentum 139 G664 gi82308 3.20E−74 Antirrhinum majus myb protein 308-garden snapdragon. 139 G664 gi19072766 5.30E−73 Oryza sativa typical P-type R2R3 Myb protein. 139 G664 gi127579 3.80E−71 Hordeum vulgare MYB-RELATED PROTEIN HV1. 139 G664 gi227030 3.80E−71 Hordeum vulgare var. myb-related gene Hv1. distichum 139 G664 gi19386839 3.00E−69 Oryza sativa (japonica putative myb-relat cultivar-group) 139 G664 gi127582 8.10E−69 Zea mays MYB-RELATED PROTEIN ZM38. 139 G664 gi23476285 2.10E−61 Gossypioides kirkii myb-like transcription factor 1. 139 G664 gi23476281 9.10E−61 Gossypium raimondii myb-like transcription factor 1. 141 G674 BE021475 2.00E−47 Glycine max sm59a03.y1 Gm-c1028 Glycine max cDNA clone GENO 141 G674 AY104558 1.00E−43 Zea mays PCO116495 mRNA sequence. 141 G674 BE402501 3.00E−43 Triticum aestivum CSB008F03F990908 ITEC CSB Wheat Endosperm 141 G674 AW672062 2.00E−42 Sorghum bicolor LG1_354_G05.b1_A002 Light Grown 1 (LG1) Sor 141 G674 CA002506 2.00E−42 Hordeum vulgare subsp. HS07L12r HS Hordeum vulgare vulgare 141 G674 AW691296 3.00E−42 Medicago truncatula NF040A12ST1F1000 Developing stem Medica 141 G674 BM356984 2.00E−41 Triphysaria versicolor 12II-D5 Triphysaria versicolor root- 141 G674 BQ290999 2.00E−41 Pinus taeda NXRV054_D07_FNXRV (Nsf Xylem Root wood Vertica 141 G674 AW626100 3.00E−40 Lycopersicon EST320007 tomato radicle, esculentum 5 d post- 141 G674 BQ802392 6.00E−40 Triticum monococcum WHE2825_D09_G17ZS Triticum monococcum v 141 G674 gi13486737 5.20E−42 Oryza sativa putative transcription factor (myb). 141 G674 gi22093837 3.70E−41 Oryza sativa (japonica contains ESTs AU10 cultivar-group) 141 G674 gi19059 2.40E−37 Hordeum vulgare MybHv33. 141 G674 gi5139802 8.10E−37 Glycine max GmMYB29A1. 141 G674 gi1167486 1.30E−36 Lycopersicon transcription factor. esculentum 141 G674 gi82310 9.30E−36 Antirrhinum majus myb protein 330-garden snapdragon. 141 G674 gi13346188 3.20E−35 Gossypium hirsutum GHMYB25. 141 G674 gi22266673 4.00E−35 Vitis labrusca x Vitis myb-related transcription vinifera 141 G674 gi6552389 1.40E−34 Nicotiana tabacum myb-related transcription factor LBM4. 141 G674 gi15082210 1.70E−34 Fragaria x ananassa transcription factor MYB1. 143 G676 AF502295 1.00E−109 Cucumis sativus werewolf (WER) mRNA, partial cds. 143 G676 BF275643 2.00E−56 Gossypium arboreum GA_Eb0024J14f Gossypium arboreum 7-10 d 143 G676 BZ078562 3.00E−47 Brassica oleracea lkz44b07.b1 B. oleracea002 Brassica olerac 143 G676 AF034130 3.00E−42 Gossypium hirsutum MYB-like DNA-binding domain protein (Cmy 143 G676 BU830456 4.00E−42 Populus tremula × T008E08 Populus apica Populus tremuloides 143 G676 AF401220 6.00E−42 Fragaria x ananassa transcription factor MYB1 (MYB1) mRNA, 143 G676 AI771837 2.00E−41 Lycopersicon EST252937 tomato ovary, esculentum TAMU Lycope 143 G676 BE124666 4.00E−41 Medicago truncatula EST393701 GVN Medicago truncatula cDNA 143 G676 BG881996 9.00E−41 Glycine max sae92f10.y1 Gm-c1065 Glycine max cDNA clone GEN 143 G676 AF474115 2.00E−40 Zea mays typical P-type R2R3 Myb protein (Myb1) gene, parti 143 G676 gi20514371 1.10E−103 Cucumis sativus werewolf. 143 G676 gi1101770 4.10E−43 Picea mariana MYB-like transcriptional factor MBF1. 143 G676 gi23476291 2.50E−42 Gossypium raimondii myb-like transcription factor 2. 143 G676 gi2921332 3.20E−42 Gossypium hirsutum MYB-like DNA-binding domain protein. 143 G676 gi23476293 6.60E−42 Gossypium herbaceum myb-like transcription factor 2. 143 G676 gi15082210 1.10E−41 Fragaria x ananassa transcription factor MYB1. 143 G676 gi23476297 1.40E−41 Gossypioides kirkii myb-like transcription factor 3. 143 G676 gi19072734 6.00E−41 Zea mays typical P-type R2R3 Myb protein. 143 G676 gi82308 1.20E−40 Antirrhinum majus myb protein 308-garden snapdragon. 143 G676 gi1167484 3.30E−40 Lycopersicon transcription factor. esculentum 145 G680 PVU420902 1.00E−149 Phaseolus vulgaris mRNA for LHY protein. 145 G680 BH579338 8.00E−93 Brassica oleracea BOGDR44TF BOGD Brassica oleracea genomic 145 G680 AAAA01009649 3.00E−59 Oryza sativa (indica ( ) scaffold009649 cultivar-group) 145 G680 AP004460 2.00E−58 Oryza sativa (japonica ( ) chromosome 8 clo cultivar-group) 145 G680 BU868664 3.00E−56 Populus balsamifera M118F07 Populus flow subsp. trichocarpa 145 G680 BE331563 2.00E−54 Glycine max sp15d08.y1 Gm-c1042 Glycine max cDNA clone GENO 145 G680 BG524104 2.00E−49 Stevia rebaudiana 38-82 Stevia field grown leaf cDNA Stevia 145 G680 AW979367 2.00E−46 Lycopersicon EST310415 tomato root esculentum deficiency, C 145 G680 BM322287 3.00E−45 Sorghum bicolor PIC1_2_F02.b1_A002 Pathogen-infected compat 145 G680 AY103618 5.00E−45 Zea mays PCO118792 mRNA sequence. 145 G680 gi21213868 1.40E−144 Phaseolus vulgaris LHY protein. 145 G680 gi15528628 4.80E−24 Oryza sativa hypothetical protein~similar to Oryza sativa 145 G680 gi18461206 1.10E−07 Oryza sativa (japonica contains ESTs AU10 cultivar-group) 145 G680 gi18874263 6.60E−07 Antirrhinum majus MYB-like transcription factor DIVARICAT 145 G680 gi12406993 1.70E−06 Hordeum vulgare MCB1 protein. 145 G680 gi12005328 3.20E−06 Hevea brasiliensis unknown. 145 G680 gi20067661 3.40E−06 Zea mays one repeat myb transcriptional factor. 145 G680 gi6688529 1.20E−05 Lycopersicon I-box binding factor. esculentum 145 G680 gi19911577 0.00036 Glycine max syringolide-induced protein 1-3-1A. 145 G680 gi7677132 0.012 Secale cereale c-myb-like transcription factor. 147 G682 BU831849 8.00E−25 Populus tremula x T026E01 Populus apica Populus tremuloides 147 G682 BU872107 8.00E−25 Populus balsamifera Q039C07 Populus flow subsp. trichocarpa 147 G682 BM437313 1.00E−20 Vitis vinifera VVA017F06_54121 An expressed sequence tag da 147 G682 BI699876 4.00E−19 Glycine max sag49b09.y1 Gm-c1081 Glycine max cDNA clone GEN 147 G682 BH961028 1.00E−16 Brassica oleracea odj30d06.g1 B. oleracea002 Brassica olerac 147 G682 AL750151 2.00E−14 Pinus pinaster AL750151 AS Pinus pinaster cDNA clone AS06C1 147 G682 BJ476463 1.00E−13 Hordeum vulgare subsp. BJ476463 K. Sato vulgare unpublished 147 G682 AJ485557 1.00E−13 Hordeum vulgare AJ485557 S00011 Hordeum vulgare cDNA clone 147 G682 CA762299 2.00E−13 Oryza sativa (indica BR060003B10F03.ab1 IRR cultivar-group) 147 G682 CA736777 2.00E−12 Triticum aestivum wpi1s.pk008.n12 wpi1s Triticum aestivum c 147 G682 gi23476287 8.30E−12 Gossypium hirsutum myb-like transcription factor 2. 147 G682 gi23476291 8.30E−12 Gossypium raimondii myb-like transcription factor 2. 147 G682 gi23476293 8.30E−12 Gossypium herbaceum myb-like transcription factor 2. 147 G682 gi23476295 8.30E−12 Gossypioides kirkii myb-like transcription factor 2. 147 G682 gi15042120 2.20E−11 Zea luxurians CI protein. 147 G682 gi19548449 2.20E−11 Zea mays P-type R2R3 Myb protein. 147 G682 gi9954118 2.80E−11 Solanum tuberosum tuber-specific and sucrose- responsive e 147 G682 gi15042108 4.60E−11 Zea mays subsp. CI protein. parviglumis 147 G682 gi15082210 1.50E−10 Fragaria x ananassa transcription factor MYB1. 147 G682 gi22266669 1.50E−10 Vitis labrusca x Vitis myb-related transcription vinifera 149 G715 BG591677 9.00E−91 Solanum tuberosum EST499519 P. infestans- challenged leaf So 149 G715 AW776719 2.00E−89 Medicago truncatula E5T335784 DSIL Medicago truncatula cDNA 149 G715 BE208917 2.00E−87 Citrus × paradisi GF-FV-P3F5 Marsh grapefruit young flavedo 149 G715 BQ411597 1.00E−86 Gossypium arboreum GA_Ed0041B06f Gossypium arboreum 7-10 d 149 G715 BM065544 4.00E−86 Capsicum annuum KS07004F12 KS07 Capsicum annuum cDNA, mRNA 149 G715 BI701620 4.00E−83 Glycine max sai18a04.y1 Gm-c1053 Glycine max cDNA clone GEN 149 G715 BH725354 2.00E−79 Brassica oleracea BOHVO37TF BO_2_3_KB Brassica oleracea gen 149 G715 AW093662 6.00E−77 Lycopersicon EST286842 tomato mixed esculentum elicitor, BT 149 G715 AW399586 2.00E−67 Lycopersicon pennellii EST310086 L. pennellii trichome, Cor 149 G715 AC134235 8.00E−66 Oryza sativa (japonica ( ) chromosome 3 clo cultivar-group) 149 G715 gi5257260 2.00E−52 Oryza sativa Similar to sequence of BAC F7G19 from Arabid 149 G715 gi20804442 1.80E−20 Oryza sativa (japonica hypothetical prote cultivar-group) 149 G715 gi18481626 3.70E−08 Zea mays repressor protein. 149 G715 gi1778097 0.19 Pinus taeda expansin. 149 G715 gi2130105 0.44 Triticum aestivum histone H2A.4-wheat. 149 G715 gi297871 0.47 Picea abies histone H2A. 149 G715 gi5106924 0.56 Medicago truncatula putative cell wall protein. 149 G715 gi1247386 0.6 Nicotiana alata PRP2. 149 G715 gi121981 0.8 Volvox carteri HISTONE H2A-III. 149 G715 gi1708102 0.8 Chlamydomonas HISTONE H2A. reinhardtii 151 G720 BH650015 1.00E−68 Brassica oleracea BOMOG70TF BO_2_3_KB Brassica oleracea gen 151 G720 BG450227 3.00E−55 Medicago truncatula NF015E11DT1F1087 Drought Medicago trunc 151 G720 BG642566 7.00E−50 Lycopersicon EST510760 tomato esculentum shoot/meristem Lyc 151 G720 BG887673 3.00E−45 Solanum tuberosum EST513524 cSTD Solanum tuberosum cDNA clo 151 G720 BU878634 5.00E−45 Populus balsamifera V049F07 Populus flow subsp. trichocarpa 151 G720 BQ594416 4.00E−42 Beta vulgaris E012444-024-024-N22-SP6 MPIZ-ADIS-024-develop 151 G720 AF318581 4.00E−41 Oryza sativa putative transcription factor OsGLK1 (Glk1) mR 151 G720 AF318579 1.00E−39 Zea mays putative transcription factor GOLDEN 2 mRNA, compl 151 G720 BU004944 5.00E−37 Lactuca sativa QGG6K14.yg.ab1 QG_EFGHJ lettuce serriola Lac 151 G720 AW618051 4.00E−34 Lycopersicon pennellii EST314101 L. pennellii trichome, Cor 151 G720 gi13940496 1.20E−38 Zea mays putative transcription factor ZmGLK1. 151 G720 gi24308616 2.20E−27 Oryza sativa (japonica Putative response cultivar-group) 151 G720 gi13940498 2.10E−26 Oryza sativa putative transcription factor OsGLK1. 151 G720 gi4519671 1.10E−08 Nicotiana tabacum transfactor. 151 G720 gi6942190 3.50E−08 Mesembryanthemum CDPK substrate protein 1; C crystallinum 151 G720 gi5916207 1.90E−06 Chlamydomonas regulatory protein of P- reinhardtii starvat 151 G720 gi10198182 0.016 Cladrastis kentukea ENOD2. 151 G720 gi100216 0.02 Lycopersicon extensin class II (clone uJ-2)- esculentum 151 G720 gi169878 0.032 Sesbania rostrata nodulin. 151 G720 gi1808688 0.041 Sporobolus stapfianus hypothetical protein. 153 G736 BH959523 2.00E−65 Brassica oleracea odh52c03.b1 B. oleracea002 Brassica olerac 153 G736 BU868493 2.00E−43 Populus balsamifera M116E08 Populus flow subsp. trichocarpa 153 G736 AW648389 4.00E−38 Lycopersicon EST326843 tomato esculentum germinating seedli 153 G736 CA810654 4.00E−37 Vitis vinifera CA22LIO1IVF-E1 CA22LI Vitis vinifera cDNA cl 153 G736 BE323614 4.00E−34 Medicago truncatula NF006A11PL1F1081 Phosphate starved leaf 153 G736 BE474759 3.00E−29 Glycine max sp68c07.y1 Gm-c1044 Glycine max cDNA clone GENO 153 G736 AP005167 7.00E−28 Oryza sativa (japonica ( ) chromosome 7 clo cultivar-group) 153 G736 AAAA01004298 7.00E−28 Oryza sativa (indica ( ) scaffold004298 cultivar-group) 153 G736 CA753311 2.00E−27 Oryza sativa 00210011068.D09_0106282 29W.scf IR62266 Oryza s 153 G736 BJ471540 3.00E−27 Hordeum vulgare subsp. BJ471540 K. Sato vulgare unpublished 153 G736 gi19071625 5.30E−30 Oryza sativa (japonica putative zinc fing cultivar-group) 153 G736 gi15451553 6.50E−30 Oryza sativa Putative H-protein promoter binding factor-2 153 G736 gi21538791 1.70E−27 Hordeum vulgare subsp. dof zinc finger protein. vulgare 153 G736 gi1669341 1.20E−26 Cucurbita maxima AOBP (ascorbate oxidase promoter-binding 153 G736 gi3929325 1.00E−22 Dendrobium grex putative DNA-binding prot Madame Thong-In 153 G736 gi3777436 1.30E−22 Hordeum vulgare DNA binding protein. 153 G736 gi2393775 1.20E−21 Zea mays prolamin box binding factor. 153 G736 gi1360078 2.40E−21 Nicotiana tabacum Zn finger protein. 153 G736 gi3790264 3.90E−21 Triticum aestivum PBF protein. 153 G736 gi7688355 6.40E−21 Solanum tuberosum Dof zinc finger protein. 155 G748 D45066 6.00E−91 Cucurbita maxima mRNA for AOBP (ascorbate oxidase promoter- 155 G748 BH530891 3.00E−69 Brassica oleracea BOHIF05TR BOHI Brassica oleracea genomic 155 G748 AP001383 3.00E−63 Oryza sativa genomic DNA, chromosome 1, clone: P0453A06. 155 G748 AAAA01004298 1.00E−62 Oryza sativa (indica ( ) scaffold004298 cultivar-group) 155 G748 AP005167 1.00E−62 Oryza sativa (japonica ( ) chromosome 7 clo cultivar-group) 155 G748 CA783807 2.00E−56 Glycine max sat57f01.y1 Gm-c1056 Glycine max cDNA clone SOY 155 G748 AC137986 1.00E−48 Medicago truncatula clone mth2-7g6, WORKING DRAFT SEQUENCE, 155 G748 AW029804 1.00E−46 Lycopersicon EST273059 tomato callus, esculentum TAMU Lycop 155 G748 BQ488386 3.00E−46 Beta vulgaris 43-E8885-006-003-F11-T3 Sugar beet MPIZ-ADIS- 155 G748 HVU312330 2.00E−41 Hordeum vulgare subsp. Hordeum vulgare partial dof vulgare 155 G748 gi1669341 5.90E−89 Cucurbita maxima AOBP (ascorbate oxidase promoter-binding 155 G748 gi7242908 1.80E−64 Oryza sativa ESTs C23582(S11122), AU056531 (S20663) corresp 155 G748 gi19071625 5.80E−59 Oryza sativa (japonica putative zinc fing cultivar-group) 155 G748 gi21538791 7.10E−38 Hordeum vulgare subsp. dof zinc finger protein. vulgare 155 G748 gi2393775 8.00E−30 Zea mays prolamin box binding factor. 155 G748 gi3929325 3.10E−28 Dendrobium grex putative DNA-binding prot Madame Thong-In 155 G748 gi3777436 5.90E−25 Hordeum vulgare DNA binding protein. 155 G748 gi3790264 2.40E−24 Triticum aestivum PBF protein. 155 G748 gi7688355 3.50E−24 Solanum tuberosum Dof zinc finger protein. 155 G748 gi6092016 1.00E−23 Pisum sativum elicitor-responsive Dof protein ERDP. 157 G779 AAAA01003354 3.00E−37 Oryza sativa (indica ( ) scaffold003354 cultivar-group) 157 G779 AP004462 3.00E−37 Oryza sativa (japonica ( ) chromosome 8 clo cultivar-group) 157 G779 AT002234 1.00E−36 Brassica rapa subsp. AT002234 Flower bud pekinensis cDNA Br 157 G779 BH775806 8.00E−36 Zeamays fzmb011f08c05f1 fzmb filtered library Zea mays ge 157 G779 CA783614 3.00E−32 Glycine max sat50g04.y1 Gm-c1056 Glycine max cDNA clone SOY 157 G779 BH650724 2.00E−30 Brassica oleracea BOMIW43TR BO_2_3_KB Brassica oleracea gen 157 G779 BE451174 6.00E−28 Lycopersicon EST402062 tomato root, esculentum plants pre-a 157 G779 AP004693 6.00E−28 Oryza sativa chromosome 8 clone P0461F06, *** SEQUENCING IN 157 G779 BF263465 4.00E−23 Hordeum vulgare HV_CEa0006N02f Hordeum vulgare seedling gre 157 G779 BG557011 3.00E−21 Sorghum bicolor EM1_41_E02.g1_A002 Embryo 1 (EM1) Sorghum b 157 G779 gi19571105 8.40E−28 Oryza sativa (japonica hypothetical prote cultivar-group) 157 G779 gi15528743 9.10E−26 Oryza sativa contains EST C74560(E31855)~unknown protein. 157 G779 gi1086534 1.90E−07 Oryza officinalis transcriptional activator Ra homolog. 157 G779 gi1086536 4.40E−07 Oryza rufipogon transcriptional activator Ra homolog. 157 G779 gi527665 5.70E−07 Sorghum bicolor myc-like regulatory R gene product. 157 G779 gi1086526 9.80E−07 Oryza australiensis transcriptional activator Ra homolog. 157 G779 gi1086530 1.30E−06 Oryza longistaminata transcriptional activator Ra homolog 157 G779 gi527661 1.70E−06 Phyllostachys acuta myc-like regulatory R gene product. 157 G779 gi3127045 2.20E−06 Petunia x hybrida bHLH transcription factor JAF13. 157 G779 gi527655 2.90E−06 Pennisetum glaucum myc-like regulatory R gene product. 159 G789 BU866069 9.00E−47 Populus tremula x S062C11 Populus imbib Populus tremuloides 159 G789 BG591063 4.00E−40 Solanum tuberosum EST498905 P. infestans- challenged leaf So 159 G789 BH593748 7.00E−36 Brassica oleracea BOGES09TR BOGE Brassica oleracea genomic 159 G789 BM411362 2.00E−35 Lycopersicon EST585689 tomato breaker esculentum fruit Lyco 159 G789 BF518953 2.00E−34 Medicago truncatula EST456346 DSIL Medicago truncatula cDNA 159 G789 BG041496 6.00E−34 Glycine max sv35a08.y1 Gm-c1057 Glycine max cDNA clone GENO 159 G789 BE598711 6.00E−30 Sorghum bicolor PI1_81_D03.b1_A002 Pathogen induced 1 (PI1) 159 G789 BU574318 6.00E−30 Prunus dulcis PA_Ea0007A10f Almond developing seed Prunus 159 G789 CA008614 6.00E−30 Hordeum vulgare subsp. HU11I14r HU Hordeum vulgare vulgare 159 G789 BG052163 3.00E−28 Sorghum propinquum RHIZ2_6_H10.b1_A003 Rhizome2 (RHIZ2) Sor 159 G789 gi23495742 5.00E−37 Oryza sativa (japonica putative phytochro cultivar-group) 159 G789 gi12957703 5.90E−26 Oryza sativa putative phytochrome interacting factor. 159 G789 gi5923912 2.70E−10 Tulipa gesneriana bHLH transcription factor GBOF-1. 159 G789 gi1086538 6.70E−09 Oryza rufipogon transcriptional activator Rb homolog. 159 G789 gi527657 1.80E−08 Pennisetum glaucum myc-like regulatory R gene product. 159 G789 gi527665 6.30E−08 Sorghum bicolor myc-like regulatory R gene product. 159 G789 gi527661 1.00E−07 Phyllostachys acuta myc-like regulatory R gene product. 159 G789 gi13346180 2.30E−07 Gossypium hirsutum GHDEL61. 159 G789 gi4206118 2.70E−07 Mesembryanthemum transporter homolog. crystallinum 159 G789 gi527663 2.80E−07 Tripsacum australe myc-like regulatory R gene product. 161 G801 BH690524 1.00E−100 Brassica oleracea BOMFD23TR BO_2_3_KB Brassica oleracea gen 161 G801 BQ401569 2.00E−59 Gossypium arboreum GA_Ed0005G12f Gossypium arboreum 7-10 d 161 G801 AF411807 2.00E−59 Lycopersicon BAC clone Clemson_Id esculentum 127E11, comple 161 G801 BG647366 2.00E−56 Medicago truncatula EST508985 HOGA Medicago truncatula cDNA 161 G801 AP004776 6.00E−55 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 161 G801 BQ741451 4.00E−48 Glycine max saq18f10.y1 Gm-c1045 Glycine max cDNA clone SOY 161 G801 BE344238 5.00E−48 Solanum tuberosum EST409400 potato stolon, Cornell Universi 161 G801 BQ791490 2.00E−38 Brassica rapa subsp. E4414 Chinese cabbage pekinensis etiol 161 G801 AC114983 2.00E−37 Oryza sativa chromosome 3 clone OSJNBa0032H19, *** SEQUENCI 161 G801 BF717245 4.00E−37 Prunus persica Lf583 near-ripe peach fruit cDNA library Pru 161 G801 gi20975251 2.40E−33 Oryza sativa (japonica transcription fact cultivar-group) 161 G801 gi5731257 1.30E−30 Gossypium hirsutum auxin-induced basic helix- loop-helix t 161 G801 gi2580440 5.80E−27 Oryza sativa PCF2. 161 G801 gi13649864 3.00E−06 Capillipedium teosinte branched1 protein. parviflorum 161 G801 gil3649873 3.00E−06 Bothriochloa odorata teosinte branched1 protein. 161 G801 gi21624275 6.20E−06 Pueraria montana var. PlCYC1. lobata 161 G801 gi6358622 3.70E−05 Digitalis purpurea cyc4 protein. 161 G801 gi6358625 3.70E−05 Misopates orontium cyc4 protein. 161 G801 gi21624285 6.70E−05 Sophora flavescens SfCYC2. 161 G801 gi6358621 6.90E−05 Antirrhinum majus cyc4 protein. subsp. cirrhigerum 163 G849 CRO251686 1.00E−126 Catharanthus roseus mRNA for MYB-like DNA- binding protein 163 G849 AF543195 1.00E−117 Nicotiana glutinosa telomere binding protein TBP1 mRNA, com 163 G849 HSBPF1 1.00E−111 Petroselinum crispum P. crispum BPF-1 mRNA. 163 G849 ZMIBP2 7.00E−89 Zea mays Z. mays IBP2 mRNA for initiator-binding protein. 163 G849 CA815602 8.00E−69 Vitis vinifera CA12EI204IIF_C11 Cabernet Sauvignon Leaf-C 163 G849 BM359662 6.00E−68 Gossypium arboreum GA_Ea0022I07r Gossypium arboreum 7-10 d 163 G849 AF242298 3.00E−66 Oryza sativa telomere binding protein-1 mRNA, complete cds. 163 G849 BU816704 5.00E−65 Populus tremula x N070D06 Populus bark Populus tremuloides 163 G849 BH443698 2.00E−57 Brassica oleracea BOGWU55TF BOGW Brassica oleracea genomic 163 G849 BE432238 5.00E−52 Lycopersicon EST398767 tomato breaker esculentum fruit, TIG 163 G849 gi12043533 7.30E−129 Catharanthus roseus MYB-like DNA-binding protein. 163 G849 gi23664357 3.10E−118 Nicotiana glutinosa telomere binding protein TBP1. 163 G849 gi2129918 1.60E−100 Petroselinum crispum BPF-1 protein-parsley. 163 G849 gi1076813 2.60E−93 Zea mays initiator-binding protein- maize. 163 G849 gi9716453 4.20E−71 Oryza sativa telomere binding protein-1; TBP1. 163 G849 gi20804653 0.46 Oryza sativa (japonica histone H1-like pr cultivar-group) 163 G849 gi15148918 0.85 Phaseolus vulgaris homeodomain leucine zipper protein HDZ 163 G849 gi126240 0.93 Sesbania rostrata Leghemoglobin 2 (Srglb2). 163 G849 gi15723363 0.97 Musa acuminata calmodulin-like protein. 163 G849 gi19073328 1 Sorghum bicolor typical P-type R2R3 Myb protein. 165 G859 AY036888 4.00E−55 Brassica napus MADS-box protein (FLC1) mRNA, complete cds. 165 G859 BG544805 3.00E−37 Brassica rapa subsp. E2809 Chinese cabbage pekinensis etiol 165 G859 BM436799 4.00E−36 Vitis vinifera VVA010B05_53181 An expressed sequence tag da 165 G859 BG596731 7.00E−36 Solanum tuberosum EST495409 cSTS Solanum tuberosum cDNA clo 165 G859 AW219962 2.00E−35 Lycopersicon EST302445 tomato root esculentum during/after 165 G859 BQ994287 2.00E−31 Lactuca sativa QGF6N05.yg.ab1 QG_EFGHJ lettuce serriola Lac 165 G859 BI957545 2.00E−30 Hordeum vulgare HVSMEn0010B09f Hordeum vulgare rachis EST 1 165 G859 BU875165 2.00E−30 Populus balsamifera V003A12 Populus flow subsp. trichocarpa 165 G859 BJ213269 3.00E−30 Triticum aestivum BJ213269 Y. Ogihara unpublished cDNA libr 165 G859 MDU78949 8.00E−30 Malus x domestica Malus domestica MADS- box protein 3 mRNA, 165 G859 gi17933450 2.70E−54 Brassica napus MADS-box protein. 165 G859 gi5777904 9.90E−32 Malus x domestica MADS-box protein 3. 165 G859 gi3646324 1.60E−31 Malus domestica MADS-box protein. 165 G859 gi9367313 2.60E−31 Hordeum vulgare MADS-box protein 8. 165 G859 gi6467974 5.50E−31 Dendrobium grex MADS box protein Madame Thong-In DOMADS2. 165 G859 gi12002141 2.40E−30 Zea mays MADS box protein 3. 165 G859 gi13446154 2.40E−30 Pisum sativum MADS-box transcription factor. 165 G859 gi4204234 2.40E−30 Lolium temulentum MADS-box protein 2. 165 G859 gi6651033 2.40E−30 Capsicum annuum MADS box transcription factor MADS1. 165 G859 gi1483232 4.90E−30 Betula pendula MADS5 protein. 167 G864 BH472654 1.00E−105 Brassica oleracea BOHPF07TF BOHP Brassica oleracea genomic 167 G864 AP004902 2.00E−44 Lotus japonicus genomic DNA, chromosome 2, clone: LjT04G24, 167 G864 BM886518 5.00E−40 Glycine max sam17f08.y1 Gm-c1068 Glycine max cDNA clone SOY 167 G864 AW685524 5.00E−39 Medicago truncatula NF031C12NR1F1000 Nodulated root Medicag 167 G864 AP001800 6.00E−36 Oryza sativa genomic DNA, chromosome 1, PAC clone: P0443E05. 167 G864 LEU89257 6.00E−32 Lycopersicon DNA-binding protein Pti6 esculentum mRNA, comp 167 G864 AAAA01000263 7.00E−31 Oryza sativa (indica ( ) scaffold000263 cultivar-group) 167 G864 BQ873772 8.00E−30 Lactuca sativa QGI2I03.yg.ab1 QG_ABCDI lettuce salinas Lact 167 G864 AF058827 7.00E−29 Nicotiana tabacum TSI1 (Tsi1) mRNA, complete cds. 167 G864 BZ419846 3.00E−25 Zea mays if61a07.b1 WGS-ZmaysF (DH5a methyl filtered) Zea m 167 G864 gi8096469 1.60E−38 Oryza sativa Similar to Arabidopsis thaliana chromosome 4 167 G864 gi2213785 1.00E−34 Lycopersicon Pti6. esculentum 167 G864 gi23617235 3.70E−25 Oryza sativa (japonica contains ESTs AU16 cultivar-group) 167 G864 gi3065895 7.60E−25 Nicotiana tabacum TSI1. 167 G864 gi3264767 1.90E−21 Prunus armeniaca AP2 domain containing protein. 167 G864 gi8571476 4.30E−21 Atriplex hortensis apetala2 domain-containing protein. 167 G864 gi17385636 2.80E−20 Matricaria chamomilla ethylene-responsive element binding 167 G864 gi8809571 4.50E−20 Nicotiana sylvestris ethylene-responsive element binding 167 G864 gi7528276 5.70E−20 Mesembryanthemum AP2-related transcription f crystallinum 167 G864 gi21908036 9.30E−20 Zea mays DRE binding factor 1. 169 G867 BQ971511 2.00E−94 Helianthus annuus QHB7E05.yg.ab1 QH_ABCDI sunflower RHA801 169 G867 AP003450 6.00E−85 Oryza sativa chromosome 1 clone P0034C09, *** SEQUENCING IN 169 G867 AC135925 1.00E−80 Oryza sativa (japonica ( ) chromosome 5 clo cultivar-group) 169 G867 AAAA01000997 1.00E−79 Oryza sativa (indica ( ) scaffold000997 cultivar-group) 169 G867 BQ405698 2.00E−77 Gossypium arboreum GA_Ed0085H02f Gossypium arboreum 7-10 d 169 G867 BZ015521 4.00E−69 Brassica oleracea oeg86a05.g1 B. oleracea002 Brassica olerac 169 G867 BF520598 2.00E−66 Medicago truncatula EST458071 DSIL Medicago truncatula cDNA 169 G867 BU994579 4.00E−64 Hordeum vulgare subsp. HM07I08r HM Hordeum vulgare vulgare 169 G867 BF424857 2.00E−62 Glycine max su59h03.y1 Gm-c1069 Glycine max cDNA clone GENO 169 G867 BU871082 1.00E−61 Populus balsamifera Q026F06 Populus flow subsp. trichocarpa 169 G867 gi18565433 2.40E−85 Oryza sativa (japonica DNA-binding protei cultivar-group) 169 G867 gi12328560 2.90E−73 Oryza sativa putative DNA binding protein RAV2. 169 G867 gi10798644 7.30E−13 Nicotiana tabacum AP2 domain-containing transcription fac 169 G867 gi18266198 2.50E−10 Narcissus AP-2 domain containing pseudonarcissus protein. 169 G867 gi20340233 2.50E−10 Thellungiella halophila ethylene responsive element bindi 169 G867 gi22074046 1.50E−09 Lycopersicon transcription factor JERF1. esculentum 169 G867 gi3264767 6.90E−09 Prunus armeniaca AP2 domain containing protein. 169 G867 gi18496063 7.10E−09 Fagus sylvatica ethylene responsive element binding prote 169 G867 gi13173164 8.30E−09 Pisum sativum APETAL2-like protein. 169 G867 gi1730475 8.70E−09 Hordeum vulgare viviparous-1. 171 G869 BH591758 7.00E−65 Brassica oleracea BOHET60TR BOHE Brassica oleracea genomic 171 G869 BQ791746 1.00E−25 Brassica rapa subsp. E3454 Chinese cabbage pekinensis etiol 171 G869 BF279235 2.00E−24 Gossypium arboreum GA_Eb0037N14f Gossypium arboreum 7-10 d 171 G869 AAAA01006972 2.00E−20 Oryza sativa (indica ( ) scaffold006972 cultivar-group) 171 G869 AP005687 2.00E−20 Oryza sativa (japonica ( ) chromosome 9 Clo cultivar-group) 171 G869 BQ483158 9.00E−20 Triticum aestivum WHE3505_C09_E17ZS Wheat unstressed root c 171 G869 BQ591872 2.00E−19 Beta vulgaris E012583-024-016-N20-SP6 MPIZ-ADIS-024-storage 171 G869 BM731589 6.00E−19 Glycine max sal81f11.y1 Gm-c1063 Glycine max cDNA clone SOY 171 G869 LEU89257 2.00E−18 Lycopersicon DNA-binding protein Pti6 esculentum mRNA, comp 171 G869 AP002526 6.00E−18 Oryza sativa genomic DNA, chromosome 1, PAC clone: P0504H10. 171 G869 gi2213785 3.40E−22 Lycopersicon Pti6. esculentum 171 G869 gi9049421 3.10E−21 Oryza sativa ESTs AU093391(E60370), AU091593 (C60458), AU09 171 G869 gi3065895 3.90E−21 Nicotiana tabacum TSI1. 171 G869 gi21908036 5.00E−16 Zea mays DRE binding factor 1. 171 G869 gi8571476 1.00E−15 Atriplex hortensis apetala2 domain-containing protein. 171 G869 gi18496063 2.60E−15 Fagus sylvatica ethylene responsive element binding prote 171 G869 gi20340233 1.60E−14 Thellungiella halophila ethylene responsive element bindi 171 G869 gi20160854 1.90E−14 Oryza sativa (japonica hypothetical prote cultivar-group) 171 G869 gi4099914 2.00E−14 Stylosanthes hamata ethylene-responsive element binding p 171 G869 gi8809573 2.00E−14 Nicotiana sylvestris ethylene-responsive element binding 173 G877 LES303343 1.00E−172 Lycopersicon mRNA for hypothetical esculentum protein (ORF 173 G877 AB063576 1.00E−168 Nicotiana tabacum NtWRKY-9 mRNA for WRKY DNA-binding protei 173 G877 IPBSPF1P 4.00E−83 Ipomoea batatas Sweet potato mRNA for SPF1 protein, complet 173 G877 AX192164 1.00E−81 Triticum aestivum Sequence 11 from Patent WO0149840. 173 G877 BZ061564 2.00E−79 Brassica oleracea 11f03c03.b1 B. oleracea002 Brassica olerac 173 G877 AX192162 1.00E−78 Glycine max Sequence 9 from Patent WO0149840. 173 G877 AF439274 2.00E−75 Retama raetam WRKY-like drought- induced protein (WRK) mRNA, 173 G877 AF459793 2.00E−75 Oryza sativa (indica ( ) WRKY transcription cultivar-group) 173 G877 OSJN00012 7.00E−75 Oryza sativa chromosome 4 clone OSJNBa0089K21, *** SEQUENC 173 G877 PCU48831 2.00E−71 Petroselinum crispum DNA-binding protein WRKY1 mRNA, comple 173 G877 gi13620227 2.80E−165 Lycopersicon hypothetical protein. esculentum 173 G877 gi14530687 4.00E−122 Nicotiana tabacum WRKY DNA-binding protein. 173 G877 gi4894965 3.30E−72 Avena sativa DNA-binding protein WRKY1. 173 G877 gi7484759 4.10E−71 Cucumis sativus SP8 binding protein homolog-cucumber. 173 G877 gi23305051 3.70E−70 Oryza sativa (indica WRKY transcription f cultivar-group) 173 G877 gi1159877 1.40E−69 Avena fatua DNA-binding protein. 173 G877 gi1076685 7.40E−57 Ipomoea batatas SPF1 protein-sweet potato. 173 G877 gi13236649 4.10E−53 Oryza sativa putative DNA-binding protein. 173 G877 gi16588566 1.20E−50 Solanum dulcamara thermal hysteresis protein STHP-64. 173 G877 gi18158619 2.10E−50 Retama raetam WRKY-like drought- induced protein. 175 G881 AB028022 4.00E−58 Nicotiana tabacum wizz mRNA, complete cds. 175 G881 AF204925 4.00E−58 Petroselinum crispum transcription factor WRKY4 (WRKY4) mRN 175 G881 BG582712 6.00E−55 Medicago truncatula EST484458 GVN Medicago truncatula cDNA 175 G881 BI935985 8.00E−49 Lycopersicon EST555874 tomato flower, esculentum anthesis L 175 G881 BG543269 4.00E−47 Brassica rapa subsp. E0763 Chinese cabbage pekinensis etiol 175 G881 BM520933 1.00E−46 Glycine max sal32c10.y1 Gm-c1059 Glycine max cDNA clone SOY 175 G881 BM404915 4.00E−45 Solanum tuberosum EST579242 potato roots Solanum tuberosum 175 G881 BU812081 1.00E−44 Populus tremula x UL92TA06 Populus leaf Populus tremuloides 175 G881 AW561928 5.00E−42 Gossypium hirsutum IPPGHZ0017 Cotton fiber and embryo Lambd 175 G881 BG525752 5.00E−42 Stevia rebaudiana 49-34 Stevia field grown leaf cDNA Stevia 175 G881 gi6472585 1.10E−60 Nicotiana tabacum WIZZ. 175 G881 gi11493822 3.30E−59 Petroselinum crispum transcription factor WRKY4. 175 G881 gi1159879 7.60E−44 Avena fatua DNA-binding protein. 175 G881 gi5042446 1.40E−31 Oryza sativa putative WRKY DNA binding protein. 175 G881 gi20160973 3.80E−24 Oryza sativa (japonica hypothetical prote cultivar-group) 175 G881 gi18158619 1.70E−21 Retama raetam WRKY-like drought- induced protein. 175 G881 gi13620227 3.50E−16 Lycopersicon hypothetical protein. esculentum 175 G881 gi1076685 4.50E−15 Ipomoea batatas SPF1 protein-sweet potato. 175 G881 gi23305051 6.10E−15 Oryza sativa (indica WRKY transcription f cultivar-group) 175 G881 gi3420906 6.70E−15 Pimpinella brachycarpa zinc finger protein; WRKY1. 177 G892 AP004125 8.00E−38 Oryza sativa chromosome 2 clone OJ1767_D02, *** SEQUENCING 177 G892 AAAA01003485 7.00E−37 Oryza sativa (indica ( ) scaffold003485 cultivar-group) 177 G892 AP004687 7.00E−37 Oryza sativa (japonica ( ) chromosome 6 clo cultivar-group) 177 G892 BH494985 3.00E−36 Brassica oleracea BOHQZ69TR BOHQ Brassica oleracea genomic 177 G892 AC135799 4.00E−33 Medicago truncatula clone mth2-11f14, WORKING DRAFT SEQUENC 177 G892 BE515999 3.00E−31 Triticum aestivum WHE0607_F08_L15ZA Wheat ABA-treated embry 177 G892 BE598018 2.00E−30 Sorghum bicolor PI1_68_F02.g1_A002 Pathogen induced 1 (PI1) 177 G892 AF411807 6.00E−30 Lycopersicon BAC clone Clemson_Id esculentum 127E11, comple 177 G892 BQ163187 8.00E−30 Zea mays 952045H12.y2 952-BMS tissue from Walbot Lab (red 177 G892 AV837063 8.00E−30 Hordeum vulgare subsp. AV837063 K. Sato vulgare unpublished 177 G892 gi18087865 2.10E−34 Oryza sativa putative zinc finger protein. 177 G892 gi19571000 3.10E−32 Oryza sativa (japonica hypothetical prote cultivar-group) 177 G892 gi4651204 8.10E−17 Cicer arietinum ring finger protein. 177 G892 gi23386073 3.00E−15 Tulipa gesneriana unnamed protein product. 177 G892 gi22597166 2.40E−08 Glycine max RING-H2 finger protein. 177 G892 gi20340241 9.80E−08 Thellungiella halophila putative RING zinc finger protein 177 G892 gi2894379 4.30E−06 Hordeum vulgare ring finger protein. 177 G892 gi12003386 6.60E−06 Nicotiana tabacum Avr9/Cf-9 rapidly elicited protein 132. 177 G892 gi18092342 1.00E−05 Zea mays ring-H2 zinc finger protein. 177 G892 gi6650528 1.30E−05 Oryza sativa subsp. putative transcription facto japonica 179 G896 BE412616 1.00E−116 Hordeum vulgare MCG002.A02R990625 ITEC MCG Barley Leaf/Culm 179 G896 BQ863573 1.00E−104 Lactuca sativa QGC24E01.yg.ab1 QG_ABCDI lettuce salinas Lac 179 G896 BQ970528 1.00E−101 Helianthus annuus QHB42F12.yg.ab1 QH_ABCDI sunflower RHA801 179 G896 AW255156 4.00E−93 Mentha x piperita ML1467 peppermint glandular trichome Ment 179 G896 BG445951 2.00E−88 Gossypium arboreum GA_Ea0030C19f Gossypium arboreum 7-10 d 179 G896 BQ740879 5.00E−86 Glycine max sap88e03.y1 Gm-c1045 Glycine max cDNA clone SOY 179 G896 AW030182 2.00E−83 Lycopersicon EST273437 tomato callus, esculentum TAMU Lycop 179 G896 BG241113 8.00E−82 Sorghum bicolor OV1_38_D04.b1_A002 Ovary 1 (OV1) Sorghum bi 179 G896 AI727328 1.00E−79 Gossypium hirsutum BNLGHi7759 Six-day Cotton fiber Gossypiu 179 G896 AAAA01012924 6.00E−77 Oryza sativa (indica ( ) scaffold012924 cultivar-group) 179 G896 gi12597889 1.10E−128 Oryza sativa hypothetical protein. 179 G896 gi4235430 2.80E−30 Hevea brasiliensis latex-abundant protein. 179 G896 gi20804732 5.40E−28 Oryza sativa (japonica putative latex-abu cultivar-group) 179 G896 gi23343885 4.20E−26 Lycopersicon metacaspase 1. esculentum 179 G896 gi17981380 2.30E−06 Brassica oleracea zinc finger protein LSD2. 179 G896 gi13509837 5.20E−06 Zea mays unnamed protein product. 179 G896 gi21992 0.0076 Volvox carteri extensin. 179 G896 gi2108256 0.011 Bromheadia extensin. finlaysoniana 179 G896 gi1076211 0.074 Chlamydomonas hypothetical protein VSP-3- reinhardtii Ch 179 G896 gi1903264 0.11 Pisum sativum hypothetical protein. 181 G910 BZ003194 2.00E−57 Brassica oleracea oef80b08.g1 B. oleracea002 Brassica olerac 181 G910 BQ865099 1.00E−32 Lactuca sativa QGC28L18.yg.ab1 QG_ABCDI lettuce salinas Lac 181 G910 AB001888 2.00E−29 Oryza sativa mRNA for zinc finger protein, complete cds, 181 G910 BU578283 1.00E−27 Glycine max sar50h06.y1 Gm-c1074 Glycine max cDNA clone SOY 181 G910 AP005113 2.00E−25 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 181 G910 BE558327 4.00E−25 Hordeum vulgare HV_CEb0017D19f Hordeum vulgare seedling gre 181 G910 BJ209915 4.00E−25 Triticum aestivum BJ209915 Y. Ogihara unpublished cDNA libr 181 G910 BU044949 5.00E−25 Prunus persica PP_LEa0021A05f Peach developing fruit mesoca 181 G910 AAAA01003074 5.00E−25 Oryza sativa (indica ( ) scaffold003074 cultivar-group) 181 G910 BQ121038 3.00E−24 Solanum tuberosum EST606614 mixed potato tissues Solanum tu 181 G910 gi3618320 1.30E−39 Oryza sativa zinc finger protein. 181 G910 gi22854986 2.30E−14 Brassica nigra COL1 protein. 181 G910 gi23495871 5.60E−14 Oryza sativa (japonica putative zinc-fing cultivar-group) 181 G910 gi10946337 1.20E−12 Ipomoea nil CONSTANS-like protein. 181 G910 gi3341723 2.20E−12 Raphanus sativus CONSTANS-like 1 protein. 181 G910 gi21667475 1.10E−11 Hordeum vulgare CONSTANS-like protein. 181 G910 gi4091804 1.20E−11 Malus x domestica CONSTANS-like protein 1. 181 G910 gi4557093 4.50E−11 Pinus radiata zinc finger protein. 181 G910 gi2303681 6.30E−11 Brassica napus unnamed protein product. 181 G910 gi21655160 2.80E−06 Hordeum vulgare subsp. CONSTANS-like protein vulgare CO6. 183 G911 AI352907 1.00E−50 Brassica napus MB73-1H PZ204.BNlib Brassica napus cDNA clon 183 G911 BG543052 7.00E−28 Brassica rapa subsp. E0523 Chinese cabbage pekinensis etiol 183 G911 BQ849490 4.00E−24 Lactuca sativa QGB10A17.yg.ab1 QG_ABCDI lettuce salinas Lac 183 G911 BU891914 1.00E−23 Populus tremula P057A07 Populus petioles cDNA library Popul 183 G911 BU885427 1.00E−23 Populus tremula x R031B05 Populus root Populus tremuloides 183 G911 AW034559 3.00E−23 Lycopersicon EST278175 tomato callus, esculentum TAMU Lycop 183 G911 BZ013045 1.00E−22 Brassica oleracea oek67d06.b1 B. oleracea002 Brassica olerac 183 G911 BG269593 3.00E−22 Mesembryanthemum L0-3678T3 Ice plant crystallinum Lambda Un 183 G911 AI729600 7.00E−22 Gossypium hirsutum BNLGHi13753 Six-day Cotton fiber Gossypi 183 G911 BG726313 2.00E−21 Glycine max sae08f02.y1 Gm-c1055 Glycine max cDNA clone GEN 183 G911 gi20805085 7.20E−13 Oryza sativa (japonica hypothetical prote cultivar-group) 183 G911 gi14164467 1.20E−12 Oryza sativa hypothetical protein. 183 G911 gi20340241 5.10E−12 Thellungiella halophila putative RING zinc finger protein 183 G911 gi20152976 1.70E−11 Hordeum vulgare subsp. similar to A. thaliana C3H vulgare 183 G911 gi17016985 5.80E−11 Cucumis melo RING-H2 zinc finger protein. 183 G911 gi23451086 7.60E−11 Medicago sativa RING-H2 protein. 183 G911 gi18092342 1.40E−09 Zea mays ring-H2 zinc finger protein. 183 G911 gi12003386 6.80E−09 Nicotiana tabacum Avr9/Cf-9 rapidly elicited porotein 132. 183 G911 gi1086225 7.00E−09 Lotus japonicus RING-finger protein-Lotus japonicus. 183 G911 gi2894379 2.20E−08 Hordeum vulgare ring finger protein. 185 G912 BH498662 2.00E−93 Brassica oleracea BOGTO66TR BOGT Brassica oleracea genomic 185 G912 AF084185 2.00E−75 Brassica napus dehydration responsive element binding prote 185 G912 AF211531 1.00E−59 Nicotiana tabacum Avr9/Cf-9 rapidly elicited protein 111B 185 G912 AY034473 1.00E−55 Lycopersicon putative transcriptional esculentum activator 185 G912 BG321601 4.00E−53 Descurainia sophia Ds01_01h03_R Ds01_AAFC_ECORC_cold_stress 185 G912 AB080965 9.00E−53 Prunus avium DREB1-like gene for dehydratiion responsive el 185 G912 BG590659 4.00E−51 Solanum tuberosum EST498501 P. infestans- challenged leaf So 185 G912 BG644969 1.00E−50 Medicago truncatula EST506588 KV3 Medicago truncatula cDNA 185 G912 BU016783 2.00E−49 Helianthus annuus QHE14A02.yg.ab1 QH_EFGHJ sunflower RHA280 185 G912 BU871514 1.00E−47 Populus balsamifera Q031D09 Populus flow subsp. trichocarpa 185 G912 gi5616086 5.90E−73 Brassica napus dehydration responsive element binding pro 185 G912 gi12003384 5.20E−58 Nicotiana tabacum Avr9/Cf-9 rapidly elicited protein 111B 185 G912 gi23495458 3.90E−53 Prunus avium dehydratiion responsive element binding prot 185 G912 gi18535580 2.00E−49 Lycopersicon putative transcriptional esculentum activato 185 G912 gi19071243 1.30E−45 Hordeum vulgare CRT/DRE binding factor 1. 185 G912 gi24474328 8.20E−44 Oryza sativa (japonica apetala2 domain-co cultivar-group) 185 G912 gi6983877 9.00E−38 Oryza sativa Similar to mRNA for DREB1A (AB007787). 185 G912 gi17148651 3.90E−35 Secale cereale CBF-like protein. 185 G912 gi20152903 1.40E−32 Hordeum vulgare subsp. CRT/DRE binding factor 2. vulgare 185 G912 gi17226801 2.10E−31 Triticum aestivum putative CRT/DRE-binding factor. 187 G913 AI352878 4.00E−87 Brassica napus MB72-11D PZ204.BNlib Brassica napus cDNA clo 187 G913 BH536782 1.00E−59 Brassica oleracea BOGCX29TR BOGC Brassica oleracea genomic 187 G913 AW033835 2.00E−46 Lycopersicon EST277406 tomato callus, esculentum TAMU Lycop 187 G913 BQ411166 1.00E−43 Gossypium arboreum GA_Ed0037B05f Gossypium arboreum 7-10 d 187 G913 BQ165313 5.00E−43 Medicago truncatula EST611182 KVKC Medicago truncatula cDNA 187 G913 AP006060 5.00E−43 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 187 G913 AAAA01000810 2.00E−42 Oryza sativa (indica ( ) scaffold000810 cultivar-group) 187 G913 OSJN00128 2.00E−38 Oryza sativa chromosome 4 clone OoSJNBA0088I22, *** SEQUENC 187 G913 BQ976989 3.00E−31 Helianthus annuus QHI23I22.yg.ab1 QH_ABCDI sunflower RHA801 187 G913 BQ592028 6.00E−30 Beta vulgaris E012695-024-021-K17-SP6 MPIZ-ADIS-024-develop 187 G913 gi14140155 1.60E−32 Oryza sativa putative AP2 domain transcription factor. 187 G913 gi12003382 1.40E−30 Nicotiana tabacum Avr9/Cf-9 rapidly elicited protein 111A 187 G913 gi20303570 1.40E−30 Oryza sativa (japonica putative transcrip cultivar-group) 187 G913 gi18535580 3.80E−30 Lycopersicon putative transcriptional esculentum activato 187 G913 gi23495460 4.40E−29 Prunus avium dehydration responsive element binding prote 187 G913 gi5616086 6.50E−28 Brassica napus dehydration responsive element binding pro 187 G913 gi21908034 1.40E−25 Zea mays DRE binding factor 2. 187 G913 gi19071243 1.20E−21 Hordeum vulgare CRT/DRE binding factor 1. 187 G913 gi17148649 2.30E−17 Secale cereale CBF-like protein. 187 G913 gi8571476 2.30E−17 Atriplex hortensis apetala2 domain-containing protein. 189 G922 AP004485  1.0e−999 Lotus japonicus genomic DNA, chromosome 2, clone: LjT08D14, 189 G922 AP003259 1.00E−130 Oryza sativa chromosome 1 clone P0466H10, *** SEQUENCING IN 189 G922 AAAA01000374 1.00E−130 Oryza sativa (indica ( ) scaffold000374 cultivar-group) 189 G922 BH493536 1.00E−121 Brassica oleracea BOGXB10TR BOGX Brassica oleracea genomic 189 G922 CNS08CCP 1.00E−92 Oryza sativa (japonica ( ) chromosome 12 cl cultivar-group) 189 G922 BG643567 6.00E−82 Lycopersicon EST511761 tomato esculentum shoot/meristem Lyc 189 G922 BQ124898 2.00E−81 Medicago truncatula EST610474 GLSD Medicago truncatula cDNA 189 G922 BU764181 2.00E−71 Glycine max sas53f07.y1 Gm-c1023 Glycine max cDNA clone SOY 189 G922 BG595716 3.00E−62 Solanum tuberosum EST494394 cSTS Solanum tuberosum cDNA clo 189 G922 AF378125 6.00E−55 Vitis vinifera GAI-like protein 1 (GAI1) gene, complete cds 189 G922 gi22830925 6.30E−127 Oryza sativa (japonica putative gibberell cultivar-group) 189 G922 gi13365610 3.00E−57 Pisum sativum SCARECROW. 189 G922 gi13170126 5.20E−55 Brassica napus unnamed protein product. 189 G922 gi10178637 6.30E−51 Zea mays SCARECROW. 189 G922 gi13937306 2.30E−50 Oryza sativa gibberellin-insensitive protein OsGAI. 189 G922 gi18254373 9.20E−50 Hordeum vulgare nuclear transcription factor SLN1. 189 G922 gi5640157 2.60E−49 Triticum aestivum gibberellin response modulator. 189 G922 gi20257451 3.10E−49 Calycadenia GIA/RGA-like gibberellin multiglandulosa resp 189 G922 gi13620224 1.30E−46 Lycopersicon lateral suppressor. esculentum 189 G922 gi13620166 2.20E−41 Capsella rubella hypothetical protein. 191 G926 BU573158 1.00E−56 Prunus dulcis PA_Ea0003A12f Almond developing seed Prunus 191 G926 BI310587 2.00E−55 Medicago truncatula EST5312337 GESD Medicago truncatula cDN 191 G926 BQ624240 1.00E−47 Citrus sinensis USDA-FP_01331 Ridge pineapple sweet orange 191 G926 BH443554 3.00E−44 Brassica oleracea BOHGN12TR BOHG Brassica oleracea genomic 191 G926 BNU33884 2.00E−39 Brassica napus clone bncbf-b1 CCAAT- binding factor B subuni 191 G926 BF113081 8.00E−38 Lycopersicon EST440591 tomato breaker esculentum fruit Lyco 191 G926 BG886494 2.00E−36 Solanum tuberosum EST512345 cSTD Solanum tuberosum cDNA clo 191 G926 AW472517 3.00E−36 Glycine max si26c12.y1 Gm-r1030 Glycine max cDNA clone GENO 191 G926 BQ407583 6.00E−36 Gossypium arboreum GA_Ed0108F07f Gossypium arboreum 7-10 d 191 G926 BG343051 7.00E−34 Hordeum vulgare HVSMEg0001N16f Hordeum vulgare pre- anthesis 191 G926 gi1173616 9.70E−41 Brassica napus CCAAT-binding factor B subunit homolog. 191 G926 gi2826786 1.10E−27 Oryza sativa RAPB protein. 191 G926 gi7141243 5.80E−27 Vitis riparia transcription factor. 191 G926 gi4731314 4.00E−19 Nicotiana tabacum CCAAT-binding transcription factor subu 191 G926 gi2104675 0.0061 Vicia faba transcription factor. 191 G926 gi21667471 0.64 Hordeum vulgare CONSTANS-like protein. 191 G926 gi13775107 0.67 Phaseolus vulgaris bZIP transcription factor 2. 191 G926 gi1096930 0.69 Solanum tuberosum H ATPase inhibitor. 191 G926 gi24413952 0.72 Oryza sativa (japonica putative iron supe cultivar-group) 191 G926 gi1839593 0.78 Zea mays heat shock protein 70 homolog {clone CHEM 3} [Ze 193 G961 BU879250 2.00E−81 Populus balsamifera V057G12 Populus flow subsp. trichocarpa 193 G961 BE060921 3.00E−72 Hordeum vulgare HVSMEg0013N15f Hordeum vulgare pre- anthesis 193 G961 BF098091 3.00E−70 Lycopersicon EST428612 tomato nutrient esculentum deficient 193 G961 BU547985 4.00E−69 Glycine max GM880014A10H12 Gm- r1088 Glycine max cDNA clone 193 G961 BF645892 3.00E−67 Medicago truncatula NF042G10EC1F1083 Elicited cell culture 193 G961 AP002542 2.00E−66 Oryza sativa genomic DNA, chromosome 6, PAC clone: P0679C08. 193 G961 AAAA01001925 2.00E−66 Oryza sativa (indica ( ) scaffold001925 cultivar-group) 193 G961 AP004562 2.00E−64 Oryza sativa (japonica ( ) chromosome 8 clo cultivar-group) 193 G961 BE357920 6.00E−62 Sorghum bicolor DG1_23_F03.b1_A002 Dark Grown 1 (DG1) Sorgh 193 G961 BQ483881 6.00E−61 Triticum aestivum WHE3513_F08_K15ZS Wheat unstressed root c 193 G961 gi11875152 4.00E−83 Oryza sativa putative NAM (no apical meristem) protein. 193 G961 gi24413978 2.90E−64 Oryza sativa (japonica NAM-like protein. cultivar-group) 193 G961 gi22597158 8.60E−47 Glycine max no apical meristem-like protein. 193 G961 gi15148914 1.00E−45 Phaseolus vulgaris NAC domain protein NAC2. 193 G961 gi1279640 1.70E−45 Petunia x hybrida NAM. 193 G961 gi4218537 2.40E−44 Triticum sp. GRAB2 protein. 193 G961 gi6732160 2.40E−44 Triticum monococcum unnamed protein product. 193 G961 gi6175246 2.30E−41 Lycopersicon jasmonic acid 2. esculentum 193 G961 gi14485513 1.00E−36 Solanum tuberosum putative NAC domain protein. 193 G961 gi7716952 8.40E−35 Medicago truncatula NAC1. 195 G971 AF132002 8.00E−54 Petunia x hybrida PHAP2B protein (Ap2B) mRNA, complete cds. 195 G971 AF253970 6.00E−52 Picea abies APETALA2-related transcription factor 1 (AP2L1) 195 G971 AF332215 6.00E−52 Malus x domestica transcription factor AHAP2 (AHAP2) mRNA, 195 G971 AY069953 7.00E−52 Hordeum vulgare APETALA2-like protein (AP2L1) mRNA, complet 195 G971 AF325506 3.00E−51 Pisum sativum APETAL2-like protein mRNA, complete cds. 195 G971 BI933811 4.00E−51 Lycopersicon EST553700 tomato flower, esculentum anthesis L 195 G971 BG447926 6.00E−51 Medicago truncatula NF103H07EC1F1062 Elicited cell culture 195 G971 BQ120583 1.00E−50 Solanum tuberosum EST606159 mixed potato tissues Solanum tu 195 G971 BM892891 2.00E−50 Glycine max sam49e02.y1 Gm-c1069 Glycine max cDNA clone SOY 195 G971 AF134116 3.00E−50 Hyacinthus orientalis APETALA2 protein homolog HAP2 (HAP2) 195 G971 gi21717332 6.90E−55 Malus x domestica transcription factor AHAP2. 195 G971 gi1732031 1.60E−54 Zea mays AP2 DNA-binding domain protein. 195 G971 gi24059986 1.80E−53 Oryza sativa (japonica putative indetermi cultivar-group) 195 G971 gi5360996 4.20E−53 Hyacinthus orientalis APETALA2 protein homolog HAP2. 195 G971 gi13173164 1.30E−51 Pisum sativum APETAL2-like protein. 195 G971 gi18476518 6.80E−51 Hordeum vulgare APETALA2-like protein. 195 G971 gi5081555 7.50E−50 Petunia x hybrida PHAP2A protein. 195 G971 gi11181612 2.00E−49 Picea abies APETALA2-related transcription factor 2. 195 G971 gi21069053 4.20E−22 Brassica napus AP2/EREBP transcription factor BABY BOOM2. 195 G971 gi21304227 6.40E−18 Oryza sativa ovule development aintegumenta-like protein 197 G974 BH517407 3.00E−57 Brassica oleracea BOGRR69TR BOGR Brassica oleracea genomic 197 G974 BI421315 2.00E−56 Lycopersicon EST531981 tomato callus, esculentum TAMU Lycop 197 G974 AF274033 5.00E−56 Atriplex hortensis apetala2 domain-containing protein mRNA, 197 G974 BQ115095 3.00E−55 Solanum tuberosum EST600671 mixed potato tissues Solanum tu 197 G974 BU046010 9.00E−55 Prunus persica PP_LEa0024O08f Peach developing fruit mesoca 197 G974 BQ742233 4.00E−51 Glycine max saq24d12.y1 Gm-c1045 Glycine max cDNA clone SOY 197 G974 BU870880 5.00E−49 Populus balsamifera Q019E02 Populus flow subsp. trichocarpa 197 G974 AAAA01000605 1.00E−47 Oryza sativa (indica ( ) scaffold000605 cultivar-group) 197 G974 AP005525 2.00E−47 Oryza sativa (japonica ( ) chromosome 9 clo cultivar-group) 197 G974 BU894329 2.00E−47 Populus tremula x X007E05 Populus wood Populus tremuloides 197 G974 gi8571476 1.70E−45 Atriplex hortensis apetala2 domain-containing protein. 197 G974 gi21908036 3.60E−43 Zea mays DRE binding factor 1. 197 G974 gi19920190 5.50E−31 Oryza sativa (japonica Putative AP2 domai cultivar-group) 197 G974 gi14140155 1.00E−20 Oryza sativa putative AP2 domain transcription factor. 197 G974 gi3264767 1.10E−20 Prunus armeniaca AP2 domain containing protein. 197 G974 gi3342211 2.20E−20 Lycopersicon Pti4. esculentum 197 G974 gi10798644 3.50E−20 Nicotiana tabacum AP2 domain-containing transcription fac 197 G974 gi21304712 9.30E−20 Glycine max ethylene-responsive element binding protein 1 197 G974 gi7528276 9.30E−20 Mesembryanthemum AP2-related transcription f crystallinum 197 G974 gi8809571 9.30E−20 Nicotiana sylvestris ethylene-responsive element binding 199 G975 BH477624 1.00E−69 Brassica oleracea BOGNB10TF BOGN Brassica oleracea genomic 199 G975 CA486875 3.00E−64 Triticum aestivum WHE4337_A02_A03ZS Wheat meiotic anther cD 199 G975 BI978981 2.00E−60 Rosa chinensis zD09 Old Blush petal SMART library Rosa chin 199 G975 AP004869 9.00E−60 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 199 G975 BU978490 1.00E−58 Hordeum vulgare subsp. HA13G05r HA Hordeum vulgare vulgare 199 G975 BG642554 8.00E−57 Lycopersicon EST356031 tomato flower esculentum buds, anthe 199 G975 BI958226 2.00E−54 Hordeum vulgare HVSMEn0013P17f Hordeum vulgare rachis EST 1 199 G975 BQ104740 1.00E−52 Rosa hybrid cultivar fc0212.e Rose Petals (Fragrant Cloud) 199 G975 AW705973 3.00E−51 Glycine max sk64c02.y1 Gm-c1016 Glycine max cDNA clone GENO 199 G975 AP003615 1.00E−47 Oryza sativa chromosome 6 clone P0486H12, *** SEQUENCING IN 199 G975 gi18650662 1.80E−25 Lycopersicon ethylene response factor 1. esculentum 199 G975 gi131754 2.10E−22 Lupinus polyphyllus PPLZ02 PROTEIN. 199 G975 gi3065895 9.20E−20 Nicotiana tabacum TSI1. 199 G975 gi8571476 9.30E−20 Atriplex hortensis apetala2 domain-containing protein. 199 G975 gi19920190 1.90E−19 Oryza sativa (japonica Putative AP2 domai cultivar-group) 199 G975 gi21908036 8.40E−19 Zea mays DRE binding factor 1. 199 G975 gi4099914 1.10E−18 Stylosanthes hamata ethylene-responsive element binding p 199 G975 gi10567106 1.60E−18 Oryza sativa osERF3. 199 G975 gi8809573 9.60E−18 Nicotiana sylvestris ethylene-responsive element binding 199 G975 gi7528276 1.20E−17 Mesembryanthemum AP2-related transcription f crystallinum 201 G979 AY103852 1.00E−84 Zea mays PCO068306 mRNA sequence. 201 G979 BQ625052 1.00E−79 Citrus sinensis USDA-FP_02143 Ridge pineapple sweet orange 201 G979 BZ068932 2.00E−71 Brassica oleracea lki37e06.b1 B. oleracea002 Brassica olerac 201 G979 AX555218 8.00E−70 Glycine max Sequence 3 from Patent WO02059332. 201 G979 BG595910 4.00E−67 Solanum tuberosum EST494588 cSTS Solanum tuberosum cDNA clo 201 G979 BJ178045 3.00E−66 Physcomitrella patens BJ178045 normalized ful subsp. patens 201 G979 AX555220 1.00E−65 Oryza sativa Sequence 5 from Patent WO02059332. 201 G979 AX058689 4.00E−65 Brassica napus Sequence 3 from Patent WO0075330. 201 G979 AW030921 2.00E−63 Lycopersicon EST274228 tomato callus, esculentum TAMU Lycop 201 G979 BF646396 2.00E−57 Medicago truncatula NF071F08EC1F1074 Elicited cell culture 201 G979 gi18844783 7.80E−71 Oryza sativa (japonica hypothetical prote cultivar-group) 201 G979 gi21069051 9.80E−64 Brassica napus AP2/EREBP transcription factor BABY BOOM1. 201 G979 gi21304225 2.60E−63 Oryza sativa aintegumenta-like protein. 201 G979 gi2652938 3.00E−62 Zea mays orf. 201 G979 gi11181612 4.40E−45 Picea abies APETALA2-related transcription factor 2. 201 G979 gi13173164 6.70E−45 Pisum sativum APETAL2-like protein. 201 G979 gi18476518 1.00E−43 Hordeum vulgare APETALA2-like protein. 201 G979 gi21717332 2.50E−42 Malus x domestica transcription factor AHAP2. 201 G979 gi5081555 1.40E−41 Petunia x hybrida PHAP2A protein. 201 G979 gi5360996 8.60E−33 Hyacinthus orientalis APETALA2 protein homolog HAP2. 203 G987 AC097277 1.00E−144 Oryza sativa chromosome 3 clone OSJNBa0022C08, *** SEQUENCI 203 G987 AAAA01003633 1.00E−144 Oryza sativa (indica ( ) scaffold003633 cultivar-group) 203 G987 AC137064 1.00E−115 Oryza sativa (japonica ( ) chromosome 11 cl cultivar-group) 203 G987 BZ035237 1.00E−107 Brassica oleracea oeh62d03.b1 B. oleracea002 Brassica olerac 203 G987 AY107709 1.00E−102 Zea mays PCO094187 mRNA sequence. 203 G987 BQ406287 6.00E−79 Gossypium arboreum GA_Ed0092G04f Gossypium arboreum 7-10 d 203 G987 BQ806671 4.00E−74 Triticum aestivum WHE3581_G12_N23ZS Wheat developing grains 203 G987 BQ148263 3.00E−73 Medicago truncatula NF065C10FL1F1082 Developing flower Medi 203 G987 BQ971271 3.00E−73 Helianthus annuus QHB6G17.yg.ab1 QH_ABCDI sunflower RHA801 203 G987 CA813062 1.00E−72 Vitis vinifera CA48LU08IIF-F7 CA48LU Vitis vinifera cDNA cl 203 G987 gi19571020 5.70E−135 Oryza sativa (japonica contains ESTs AU16 cultivar-group) 203 G987 gi14719332 1.70E−120 Oryza sativa putative SCARECROW gene regulator. 203 G987 gi20334379 6.10E−42 Vitis vinifera GAI-like protein 1. 203 G987 gi13170126 7.70E−41 Brassica napus unnamed protein product. 203 G987 gi20257473 1.20E−40 Dubautia raillardioides GIA/RGA-like gibberellin response 203 G987 gi20257438 1.50E−40 Argyroxiphium GIA/RGA-li sandwicense subsp. macrocephalum 203 G987 gi20257428 1.50E−40 Dubautia menziesii GIA/RGA-like gibberellin response modu 203 G987 gi20257467 1.50E−40 Dubautia arborea GIA/RGA-like gibberellin response modula 203 G987 gi20257475 1.90E−40 Dubautia microcephala GIA/RGA-like gibberellin response m 203 G987 gi20257445 1.90E−40 Carlquistia muirii GIA/RGA-like gibberellin response modu 205 G988 CRU303349  1.0e−999 Capsella rubella ORF1, ORF2, ORF3, ORF4, ORF5 and ORF6 (pa 205 G988 BH594527 1.00E−114 Brassica oleracea BOGWK18TF BOGW Brassica oleracea genomic 205 G988 LES303345 1.00E−112 Lycopersicon lateral suppressor gene, esculentum ORF1 and 205 G988 A84080 1.00E−111 Solanum tuberosum Sequence 9 from Patent WO9846759. 205 G988 AP004191 2.00E−63 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 205 G988 AAAA01001835 4.00E−62 Oryza sativa (indica ( ) scaffold001835 cultivar-group) 205 G988 AP003944 6.00E−62 Oryza sativa chromosome 6 clone OJ1126_F05, *** SEQUENCING 205 G988 AC137079 2.00E−48 Medicago truncatula clone mth2-27d17, WORKING DRAFT SEQUENC 205 G988 AF378125 4.00E−48 Vitis vinifera GAI-like protein 1 (GAI1) gene, complete cds 205 G988 AF460219 3.00E−47 Hordeum vulgare nuclear transcription factor SLN1 gene, com 205 G988 gi13620166 1.90E−211 Capsella rubella hypothetical protein. 205 G988 gi13620224 3.30E−88 Lycopersicon lateral suppressor. esculentum 205 G988 gi20334379 2.60E−53 Vitis vinifera GAI-like protein 1. 205 G988 gi13170126 4.20E−51 Brassica napus unnamed protein product. 205 G988 gi18254373 3.70E−48 Hordeum vulgare nuclear transcription factor SLN1. 205 G988 gi13603445 3.30E−47 Oryza sativa putative OsGAI. 205 G988 gi21901982 3.30E−47 Oryza sativa (japonica putative OsGAI. cultivar-group) 205 G988 gi20257451 2.90E−46 Calycadenia GIA/RGA-like gibberellin multiglandulosa resp 205 G988 gi20257422 3.70E−46 Dubautia arborea GIA/RGA-like gibberellin response modula 205 G988 gi5640157 6.60E−46 Triticum aestivum gibberellin response modulator. 207 G1040 BH494598 2.00E−52 Brassica oleracea BOGHF24TF BOGH Brassica oleracea genomic 207 G1040 BQ115343 2.00E−47 Solanum tuberosum EST600919 mixed potato tissuesSolanum tu 207 G1040 BM526051 5.00E−29 Glycine max sa136d09.y1 Gm-c1059 Glycine max cDNA clone SOY 207 G1040 CA498340 1.00E−28 Triticum aestivum WHE3242_B12_C24ZT Wheat meiotic anther cD 207 G1040 BQ280209 2.00E−28 Zea mays 1091036A08.x1 1091- Immature ear with common ESTs 207 G1040 BQ996658 4.00E−28 Lactuca sativa QGG13H02.yg.ab1 QG_EFGHJ lettuce serriola La 207 G1040 BI309203 1.00E−27 Medicago truncatula EST530613 GPOD Medicago truncatula cDNA 207 G1040 AI163121 1.00E−26 Populus tremula x A033P70U Hybrid aspen Populus tremuloides 207 G1040 AI487405 1.00E−23 Lycopersicon EST245727 tomato ovary, esculentum TAMU Lycope 207 G1040 AP005904 3.00E−19 Oryza sativa (japonica ( ) chromosome 9 clo cultivar-group) 207 G1040 gi4519671 3.10E−18 Nicotiana tabacum transfactor. 207 G1040 gi6942190 5.10E−16 Mesembryanthemum CDPK substrate protein 1; C crystallinum 207 G1040 gi23306130 5.20E−16 Oryza sativa (japonica Unknown protein. cultivar-group) 207 G1040 gi5916207 5.70E−11 Chlamydomonas regulatory protein of P- reinhardtii starvat 207 G1040 gi11034542 8.50E−08 Oryza sativa hypothetical protein~similar to Arabidopsis 207 G1040 gi14189890 9.80E−07 Zea mays response regulator 9. 207 G1040 gi2346972 0.99 Petunia x hybrida ZPT2-11. 207 G1040 gi2058313 0.99 Eucalyptus gunnii cinnamoyl-CoA reductase. 207 G1040 gi10304406 0.99 Eucalyptus saligna cinnamoyl-CoA reductase. 207 G1040 gi22597156 1 Glycine max nucleolar histone deacetylase HD2-P39. 209 G1047 BH950967 9.00E−56 Brassica oleracea odh95h11.b1 B. oleracea002 Brassica olerac 209 G1047 BU870843 4.00E−29 Populus balsamifera Q019A11 Populus flow subsp. trichocarpa 209 G1047 BF051268 1.00E−28 Lycopersicon EST436443 tomato esculentum developing/immatur 209 G1047 BM269595 1.00E−21 Glycine max sak01g11.y1 Gm-c1074 Glycine max cDNA clone SOY 209 G1047 BI977302 1.00E−11 Rosa chinensis eG09 Old Blush petal SMART library Rosa chin 209 G1047 BQ519273 2.00E−11 Solanum tuberosum EST626688 Generation of a set of potato c 209 G1047 BM437317 8.00E−11 Vitis vinifera VVA017G01_54129 An expressed sequence tag da 209 G1047 CA524885 3.00E−10 Capsicum annuum KS12044G09 KS12 Capsicum annuum cDNA, mRNA 209 G1047 AU294545 5.00E−10 Zinnia elegans AU294545 zinnia cultured mesophyll cell equa 209 G1047 AY045570 7.00E−10 Nicotiana tabacum bZIP transcription factor BZI-2 mRNA, com 209 G1047 gi13430400 9.20E−13 Phaseolus vulgaris bZip transcription factor. 209 G1047 gi16580130 1.20E−12 Nicotiana tabacum bZIP transcription factor BZI-2. 209 G1047 gi12829956 1.90E−12 Phaseolus acutifolius bZIP. 209 G1047 gi24460973 1.10E−11 Capsicum chinense bZIP transcription factor. 209 G1047 gi9650828 1.10E−11 Petroselinum crispum common plant regulatory factor 7. 209 G1047 gi12039274 3.60E−11 Oryza sativa hypothetical protein. 209 G1047 gi22597162 4.60E−11 Glycine max bZIP transcription factor ATB2. 209 G1047 gi3986151 4.80E−10 Raphanus sativus rdLIP. 209 G1047 gi5901747 4.80E−10 Lycopersicon bZIP DNA-binding protein. esculentum 209 G1047 gi2244742 4.30E−09 Antirrhinum majus bZIP DNA-binding protein. 211 G1051 BG044358 3.00E−61 Glycine max saa27d10.y1 Gm-c1059 Glycine max cDNA clone GEN 211 G1051 BF269752 1.00E−57 Gossypium arboreum GA_Eb0005I16f Gossypium arboreum 7-10 d 211 G1051 AI729411 1.00E−49 Gossypium hirsutum BNLGHi13312 Six-day Cotton fiber Gossypi 211 G1051 AL372333 7.00E−49 Medicago truncatula MtBA50C02R1 MtBA Medicago truncatula cD 211 G1051 BF051625 2.00E−47 Lycopersicon EST436861 tomato esculentum developing/immatur 211 G1051 BQ869540 2.00E−44 Lactuca sativa QGD6H14.yg.ab1 QG_ABCDI lettuce salinas Lact 211 G1051 AV426757 3.00E−44 Lotus japonicus AV426757 Lotus japonicus young plants (two- 211 G1051 BJ279680 7.00E−41 Triticum aestivum BJ279680 Y. Ogihara unpublished cDNA libr 211 G1051 AY107108 2.00E−40 Zea mays PCO062113 mRNA sequence. 211 G1051 BE420598 8.00E−39 Hordeum vulgare HWM000.E11 ITEC HWM Barley Leaf Library Hor 211 G1051 gi8096589 3.80E−46 Oryza sativa Similar to oryza sativa bZIP transcriptional 211 G1051 gi20160758 1.40E−24 Oryza sativa (japonica hypothetical prote cultivar-group) 211 G1051 gi2921823 1.10E−18 Paulownia kawakamii shoot-forming PKSF1. 211 G1051 gi8777512 7.30E−18 Nicotiana tabacum bZIP transcriptional activator RSG. 211 G1051 gi3425907 3.40E−16 Lycopersicon transcription factor VSF-1. esculentum 211 G1051 gi4586586 4.70E−16 Cicer arietinum bZIP DNA binding protein. 211 G1051 gi1060935 5.80E−09 Zea mays mLIP15. 211 G1051 gi463212 9.70E−08 Coix lacryma-jobi opaque 2. 211 G1051 gi1905785 1.40E−07 Glycine max G/HBF-1. 211 G1051 gi100163 4.30E−07 Petroselinum crispum light-induced protein CPRF- 2-parsl 213 G1052 BG044358 8.00E−66 Glycine max saa27d10.y1 Gm-c1059 Glycine max cDNA clone GEN 213 G1052 AP002092 3.00E−65 Oryza sativa genomic DNA, chromosome 1, PAC clone: P0031E09. 213 G1052 AAAA01012061 2.00E−64 Oryza sativa (indica ( ) scaffold012061 cultivar-group) 213 G1052 BF269752 2.00E−56 Gossypium arboreum GA_Eb0005I16f Gossypium arboreum 7-10 d 213 G1052 AI729411 4.00E−52 Gossypium hirsutum BNLGHi13312 Six-day Cotton fiber Gossypi 213 G1052 BF051625 3.00E−50 Lycopersicon EST436861 tomato esculentum developing/immatur 213 G1052 AL372333 2.00E−48 Medicago truncatula MtBA50C02R1 MtBA Medicago truncatula cD 213 G1052 BH529222 8.00E−48 Brassica oleracea BOHBA78TF BOHB Brassica oleracea genomic 213 G1052 AV426757 1.00E−46 Lotus japonicus AV426757 Lotus japonicus young plants (two- 213 G1052 BQ866454 3.00E−45 Lactuca sativa QGC8A11.yg.ab1 QG_ABCDI lettuce salinas Lact 213 G1052 gi8096589 8.60E−75 Oryza sativa Similar to Oryza sativa bZIP transcriptional 213 G1052 gi20160758 6.90E−43 Oryza sativa (japonica hypothetical prote cultivar-group) 213 G1052 gi2921823 1.00E−18 Paulownia kawakamii shoot-forming PKSF1. 213 G1052 gi1076603 1.10E−17 Lycopersicon vsf-1 protein-tomato. esculentum 213 G1052 gi8777512 3.80E−17 Nicotiana tabacum bZIP transcriptional activator RSG. 213 G1052 gi4586586 3.70E−14 Cicer arietinum bZIP DNA binding protein. 213 G1052 gi1060935 4.80E−09 Zea mays mLIP15. 213 G1052 gi1905785 2.50E−07 Glycine max G/HBF-1. 213 G1052 gi1076760 9.40E−07 Sorghum bicolor Opaque-2-related protein- sorghum. 213 G1052 gi9650826 9.60E−07 Petroselinum crispum common plant regulatory factor 6. 215 G1062 BQ990836 3.00E−88 Lactuca sativa QGF21D20.yg.ab1 QG_EFGHJ lettuce serriola La 215 G1062 BH470947 8.00E−84 Brassica oleracea BOGSV06TR BOGS Brassica oleracea genomic 215 G1062 BE040141 2.00E−83 Oryza sativa OD102H09 OD Oryza sativa cDNA 5′ similar to bh 215 G1062 CA501920 6.00E−80 Triticum aestivum WHE4040_D03_H06ZT Wheat meiotic anther cD 215 G1062 AW648468 1.00E−79 Lycopersicon EST326922 tomato esculentum germinating seedli 215 G1062 BU763190 1.00E−78 Glycine max sas38f03.y1 Gm-c1080 Glycine max cDNA clone SOY 215 G1062 BE602161 5.00E−70 Hordeum vulgare HVSMEh0102M15f Hordeum vulgare 5-45 DAP spi 215 G1062 BM111984 5.00E−69 Solanum tuberosum EST559520 potato roots Solanum tuberosum 215 G1062 AU291385 2.00E−68 Zinnia elegans AU291385 zinnia cultured mesophyll cell equa 215 G1062 BU983081 1.00E−57 Hordeum vulgare subsp. HA28H22r HA Hordeum vulgare vulgare 215 G1062 gi20161831 1.60E−81 Oryza sativa (japonica hypothetical prote cultivar-group) 215 G1062 gi10140754 1.40E−27 Oryza sativa hypothetical protein. 215 G1062 gi1142619 3.50E−13 Phaseolus vulgaris phaseolin G-box binding protein PG1. 215 G1062 gi527661 7.50E−12 Phyllostachys acuta myc-like regulatory R gene product. 215 G1062 gi10998404 7.50E−12 Petunia x hybrida anthocyanin 1. 215 G1062 gi1420924 1.90E−11 Zea mays IN1. 215 G1062 gi527665 3.30E−11 Sorghum bicolor myc-like regulatory R gene product. 215 G1062 gi1086526 9.10E−11 Oryza australiensis transcriptional activator Ra homolog. 215 G1062 gi1086534 1.20E−10 Oryza officinalis transcriptional activator Ra homolog. 215 G1062 gi1086538 1.20E−10 Oryza rufipogon transcriptional activator Rb homolog. 217 G1063 BH700922 1.00E−87 Brassica oleracea BOMMZ07TR BO_2_3_KB Brassica oleracea gen 217 G1063 BE451174 1.00E−43 Lycopersicon EST402062 tomato root, esculentum plants pre-a 217 G1063 AW832545 2.00E−43 Glycine max sm12e10.y1 Gm-c1027 Glycine max cDNA clone GENO 217 G1063 AP004693 5.00E−42 Oryza sativa chromosome 8 clone P0461F06, *** SEQUENCING IN 217 G1063 AAAA01006870 1.00E−39 Oryza sativa (indica ( ) scaffold006870 cultivar-group) 217 G1063 AP005655 1.00E−39 Oryza sativa (japonica ( ) chromosome 9 clo cultivar-group) 217 G1063 BH775806 2.00E−36 Zea mays fzmb011f018c05f1 fzmb filtered library Zea mays ge 217 G1063 AT002234 4.00E−34 Brassica rapa subsp. AT002234 Flower bud pekinensis cDNA Br 217 G1063 BF263465 3.00E−26 Hordeum vulgare HV_CEa0006N02f Hordeum vulgare seedling gre 217 G1063 CA015528 2.00E−25 Hordeum vulgare subsp. HT14J12r HT Hordeum vulgare vulgare 217 G1063 gi19571105 7.20E−29 Oryza sativa (japonica hypothetical prote cultivar-group) 217 G1063 gi15528743 8.90E−27 Oryza sativa contains EST C74560(E31855)~unknown protein. 217 G1063 gi6166283 1.70E−10 Pinus taeda helix-loop-helix protein 1A. 217 G1063 gi11045087 1.80E−09 Brassica napus putative protein. 217 G1063 gi10998404 1.50E−08 Petunia x hybrida anthocyanin 1. 217 G1063 gi1142621 1.10E−07 Phaseolus vulgaris phaseolin G-box binding protein PG2. 217 G1063 gi166428 1.70E−07 Antirrhinum majus DEL. 217 G1063 gi527665 8.00E−07 Sorghum bicolor myc-like regulatory R gene product. 217 G1063 gi3399777 9.40E−07 Glycine max symbiotic ammonium transporter; nodulin. 217 G1063 gi5923912 1.40E−06 Tulipa gesneriana bHLH transcription factor GBOF-1. 219 G1064 AP005733 6.00E−68 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 219 G1064 AF165924 4.00E−65 Gossypium hirsutum auxin-induced basic helix- loop-helix 219 G1064 AP003569 9.00E−59 Oryza sativa chromosome 6 clone P0425F05, *** SEQUENCING IN 219 G1064 AAAA01000293 9.00E−59 Oryza sativa (indica ( ) scaffold000293 cultivar-group) 219 G1064 BG447197 1.00E−56 Gossypium arboreum GA_Eb0041A19f Gossypium arboreum 7-10 d 219 G1064 AW649873 7.00E−54 Lycopersicon EST328327 tomato esculentum germinating seedli 219 G1064 BH652584 2.00E−48 Brassica oleracea BOMKX03TR BO_2_3_KB Brassica oleracea gen 219 G1064 AW695783 3.00E−45 Medicago truncatula NF098G07ST1F1055 Developing stem Medica 219 G1064 AV422714 4.00E−45 Lotus japonicus AV422714 Lotus japonicus young plants (two- 219 G1064 BQ294210 5.00E−42 Zea mays 1091026H05.y2 1091- Immature ear with common ESTs 219 G1064 gi5731257 9.90E−64 Gossypium hirsutum auxin-induced basic helix- loop-helix t 219 G1064 gi20975251 8.60E−45 Oryza sativa (japonica transcription fact cultivar-group) 219 G1064 gi2580440 3.00E−32 Oryza sativa PCF2. 219 G1064 gi20269127 1.70E−07 Lupinus albus TCP1 protein. 219 G1064 gi12002867 1.10E−06 Lycopersicon cycloidea. esculentum 219 G1064 gi7248461 0.00029 Zea mays root cap-specific protein. 219 G1064 gi21624279 0.00082 Pueraria montana var. PlCYC3. lobata 219 G1064 gi13649864 0.00085 Capillipedium teosinte branched1 protein. parviflorum 219 G1064 gi13649873 0.0013 Bothriochloa odorata teosinte branched1 protein. 219 G1064 gi7008009 0.0016 Pisum sativum PsAD1. 221 G1069 BZ025139 1.00E−111 Brassica oleracea oeh63d12.g1 B. oleracea002 Brassica olerac 221 G1069 AP004971 1.00E−93 Lotus japonicus genomic DNA, chromosome 5, clone: LjT45G21, 221 G1069 AP004020 2.00E−79 Oryza sativa chromosome 2 clone OJ1119_A01, *** SEQUENCING 221 G1069 AAAA01017331 2.00E−70 Oryza sativa (indica ( ) scaffold017331 cultivar-group) 221 G1069 BQ165495 2.00E−62 Medicago truncatula EST611364 KVKC Medicago truncatula cDNA 221 G1069 AC135209 2.00E−61 Oryza sativa (japonica ( ) chromosome 3 clo cultivar-group) 221 G1069 AW621455 4.00E−59 Lycopersicon EST312253 tomato root esculentum during/after 221 G1069 BM110212 4.00E−58 Solanum tuberosum EST557748 potato roots Solanum tuberosum 221 G1069 BQ785950 7.00E−58 Glycine max saq61f09.y1 Gm-c1076 Glycine max cDNA clone SOY 221 G1069 BQ863249 1.00E−57 Lactuca sativa QGC23G02.yg.ab1 QG_ABCDI lettuce salinas Lac 221 G1069 gi24059979 2.10E−38 Oryza sativa (japonica similar to DNA-bin cultivar-group) 221 G1069 gi15528814 4.50E−36 Oryza sativa hypothetical protein~similar to Arabidopsis 221 G1069 gi4165183 7.60E−25 Antirrhinum majus SAP1 protein. 221 G1069 gi2213534 1.20E−19 Pisum sativum DNA-binding PD1-like protein. 221 G1069 gi2459999 1 Chlamydomonas tubulin Uni3. reinhardtii 221 G1069 gi100872 1 Zea mays MFS18 protein-maize. 221 G1069 gi1362165 1 Hordeum vulgare hypothetical protein 2 (clone ES1A)-bar 223 G1073 AAAA01000486 4.00E−74 Oryza sativa (indica ( ) scaffold000486 cultivar-group) 223 G1073 AP004165 4.00E−74 Oryza sativa chromosome 2 clone OJ1479_B12, *** SEQUENCING 223 G1073 AP005477 2.00E−67 Oryza sativa (japonica ( ) chromosome 6 clo cultivar-group) 223 G1073 BZ412041 3.00E−65 Zea mays OGACG56TC ZM_0.7_1.5_KB Zea mays genomic clone ZMM 223 G1073 AJ502190 3.00E−64 Medicago truncatula AJ502190 MTAMP Medicago truncatula cDNA 223 G1073 BQ865858 4.00E−63 Lactuca sativa QGC6B08.yg.ab1 QG_ABCDI lettuce salinas Lact 223 G1073 BH975957 5.00E−63 Brassica oleracea odh67e11.g1 B. oleracea002 Brassica olerac 223 G1073 BG134451 8.00E−62 Lycopersicon EST467343 tomato crown esculentum gall Lycoper 223 G1073 AP004971 3.00E−60 Lotus japonicus genomic DNA, chromosome 5, clone: LjT45G21, 223 G1073 BM110212 7.00E−58 Solanum tuberosum EST557748 potato roots Solanum tuberosum 223 G1073 gi15528814 5.50E−38 Oryza sativa hypothetical protein~similar to Arabidopsis 223 G1073 gi24059979 1.30E−29 Oryza sativa (japonica similar to DNA-bin cultivar-group) 223 G1073 gi2213536 1.20E−21 Pisum sativum DNA-binding protein PD1. 223 G1073 gi4165183 5.70E−20 Antirrhinum majus SAP1 protein. 223 G1073 gi1166450 0.00059 Lycopersicon Tfm5. esculentum 223 G1073 gi11545668 0.0051 Chlamydomonas CIA5. reinhardtii 223 G1073 gi4755087 0.0054 Zea mays aluminum-induced protein; Al-induced protein. 223 G1073 gi395147 0.0068 Nicotiana tabacum glycine-rich protein. 223 G1073 gi21068672 0.017 Cicer arietinum putative glicine-rich protein. 223 G1073 gi1346181 0.017 Sinapis alba GLYCINE-RICH RNA- BINDING PROTEIN GRP2A. 225 G1075 BH596283 1.00E−108 Brassica oleracea BOGBL42TR BOGB Brassica oleracea genomic 225 G1075 BQ165495 5.00E−88 Medicago truncatula EST611364 KVKC Medicago truncatula cDNA 225 G1075 AAAA01003389 3.00E−84 Oryza sativa (indica ( ) scaffold003389 cultivar-group) 225 G1075 OSJN00182 3.00E−84 Oryza sativa chromosome 4 clone OSJNBa0086O06, *** SEQUENC 225 G1075 BZ412041 1.00E−76 Zea mays OGACG56TC ZM_0.7_1.5_KB Zea mays genomic clone ZMM 225 G1075 AP005653 1.00E−68 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 225 G1075 BQ863249 3.00E−65 Lactuca sativa QGC23G02.yg.ab1 QG_ABCDI lettuce salinas Lac 225 G1075 BM110212 2.00E−63 Solanum tuberosum EST557748 potato roots Solanum tuberosum 225 G1075 BQ838600 8.00E−63 Triticum aestivum WHE2912_D12_H24ZS Wheat aluminum-stressed 225 G1075 AP004971 4.00E−62 Lotus japonicus genomic DNA, chromosome 5, clone: LjT45G21, 225 G1075 gi15528814 3.80E−39 Oryza sativa hypothetical protein~similar to Arabidopsis 225 G1075 gi24059979 6.60E−35 Oryza sativa (japonica similar to DNA-bin cultivar-group) 225 G1075 gi4165183 7.30E−20 Antirrhinum majus SAP1 protein. 225 G1075 gi2213534 2.50E−19 Pisum sativum DNA-binding PD1-like protein. 225 G1075 gi3810890 3.70E−05 Cucumis sativus glycine-rich protein-2. 225 G1075 gi7489009 0.0001 Lycopersicon glycine-rich protein (clone esculentum w10-1 225 G1075 gi4115615 0.0018 Zea mays root cap-specific glycine- rich protein. 225 G1075 gi1628463 0.004 Silene latifolia Men-4. 225 G1075 gi395147 0.005 Nicotiana tabacum glycine-rich protein. 225 G1075 gi121631 0.0056 Nicotiana sylvestris GLYCINE-RICH CELL WALL STRUCTURAL PR 227 G1084 BH733462 5.00E−98 Brassica oleracea BOMEF84TF BO_2_3_KB Brassica oleracea gen 227 G1084 AAAA01002671 5.00E−79 Oryza sativa (indica ( ) scaffold002671 cultivar-group) 227 G1084 AP004622 5.00E−79 Oryza sativa (japonica ( ) chromosome 8 clo cultivar-group) 227 G1084 AC135313 9.00E−78 Medicago truncatula clone mth2-7n18, WORKING DRAFT SEQUENCE 227 G1084 AF268596 7.00E−41 Oryza sativa bZIP (bZIP) mRNA, complete cds. 227 G1084 BG135778 2.00E−40 Lycopersicon EST468670 tomato crown esculentum gall Lycoper 227 G1084 BQ875336 3.00E−39 Lactuca sativa QGI7N06.yg.ab1 QG_ABCDI lettuce salinas Lact 227 G1084 BQ470403 2.00E−35 Hordeum vulgare HX02O04r HX Hordeum vulgare cDNA clone HX02 227 G1084 BG651461 3.00E−33 Glycine max sad47a06.y1 Gm-c1075 Glycine max cDNA clone GEN 227 G1084 BI141172 4.00E−32 Sorghum bicolor IP1_44_A10.b1_A002 Immature pannicle 1 (IP1 227 G1084 gi20146230 1.60E−34 Oryza sativa (japonica bzip-like transcri cultivar-group) 227 G1084 gi15408647 1.80E−31 Oryza sativa putative bZIP (leucine zipper) protein. 227 G1084 gi22858664 9.00E−28 Gossypium hirsutum unknown. 227 G1084 gi13620168 0.00064 Capsella rubella hypothetical protein. 227 G1084 gi22550110 0.0017 Marsilea quadrifolia bZIP-like protein. 227 G1084 gi14329812 0.0074 Atropa belladonna putative nucleosome assembly protein 1. 227 G1084 gi2257756 0.012 Zea mays nucleolar histone deacetylase HD2-p39. 227 G1084 gi4106378 0.031 Brassica napus calcium-binding protein. 227 G1084 gi14335 0.17 Chloroplast Oenothera ORF2280. odorata 227 G1084 gi401496 0.17 Chloroplast Oenothera HYPOTHETICAL picensis PROTEIN (ORF 229 G1089 BH602457 1.00E−103 Brassica oleracea BOGCB25TR BOGC Brassica oleracea genomic 229 G1089 BQ979739 1.00E−90 Helianthus annuus QHI9B09.yg.ab1 QH_ABCDI sunflower RHA801 229 G1089 AAAA01000525 1.00E−79 Oryza sativa (indica ( ) scaffold000525 cultivar-group) 229 G1089 AP005779 1.00E−79 Oryza sativa (japonica ( ) chromosome 7 clo cultivar-group) 229 G1089 AP003931 1.00E−79 Oryza sativa chromosome 7 clone OJ1664_D08, *** SEQUENCING 229 G1089 AC135413 2.00E−78 Medicago truncatula clone mth2-16n19, WORKING DRAFT SEQUENC 229 G1089 BE659923 2.00E−76 Glycine max 1098 GmaxSC Glycine max cDNA, mRNA sequence. 229 G1089 BJ224103 8.00E−76 Triticum aestivum BJ224103 Y. Ogihara unpublished cDNA libr 229 G1089 BQ991309 2.00E−75 Lactuca sativa QGF22I10.yg.ab1 QG_EFGHJ lettuce serriola La 229 G1089 BU992003 1.00E−70 Hordeum vulgare HD08I18r HD Hordeum vulgare cDNA clone HD08 229 G1089 gi23237834 5.20E−149 Oryza sativa (japonica bZIP protein-like. cultivar-group) 229 G1089 gi15408647 3.40E−109 Oryza sativa putative bZIP (leucine zipper) protein. 229 G1089 gi22858664 5.80E−58 Gossypium hirsutum unknown. 229 G1089 gi22550110 4.70E−18 Marsilea quadrifolia bZIP-like protein. 229 G1089 gi12018147 1.80E−07 Chlamydomonas vegetative cell wall protein reinhardtii gp 229 G1089 gi1184100 1.00E−06 Nicotiana alata pistil extensin-like protein. 229 G1089 gi100216 1.50E−06 Lycopersicon extensin class II (clone uJ-2)- esculentum 229 G1089 gi6523547 4.10E−06 Volvox carteri f. hydroxyproline-rich glycopr nagariensis 229 G1089 gi18873729 4.40E−06 Saccharum hybrid proline-rich protein. cultivar CP65-357 229 G1089 gi4106378 7.30E−06 Brassica napus calcium-binding protein. 231 G1134 BF096555 6.00E−46 Lycopersicon EST360582 tomato nutrient esculentum deficient 231 G1134 BH509718 2.00E−34 Brassica oleracea BOHGV18TF BOHG Brassica oleracea genomic 231 G1134 BU091550 4.00E−33 Glycine max st74e07.y1 Gm-c1054 Glycine max cDNA clone GENO 231 G1134 BF005956 1.00E−32 Medicago truncatula EST434454 DSLC Medicago truncatula cDNA 231 G1134 BU866761 3.00E−32 Populus tremula x S070E02 Populus imbib Populus tremuloides 231 G1134 BM109038 1.00E−30 Solanum tuberosum EST556574 potato roots Solanum tuberosum 231 G1134 BM436251 1.00E−29 Vitis vinifera VVA001A07_52085 An expressed sequence tag da 231 G1134 BQ281404 3.00E−29 Triticum aestivum WHE3020_H08_P16ZS Wheat unstressed seedli 231 G1134 BU029490 5.00E−29 Helianthus annuus QHJ10N22.yg.ab1 QH_EFGHJ sunflower RHA280 231 G1134 BQ803551 8.00E−29 Triticum monococcum WHE2838_H09_O18ZS Triticum monococcum v 231 G1134 gi6166283 5.10E−35 Pinus taeda helix-loop-helix protein 1A. 231 G1134 gi20161021 6.20E−33 Oryza sativa (japonica contains ESTs AU05 cultivar-group) 231 G1134 gi19401700 1.00E−29 Oryza sativa transcription factor RAU1. 231 G1134 gi5923912 1.80E−11 Tulipa gesneriana bHLH transcription factor GBOF-1. 231 G1134 gi1086538 2.80E−06 Oryza rufipogon transcriptional activator Rb homolog. 231 G1134 gi527657 1.30E−05 Pennisetum glaucum myc-like regulatory R gene product. 231 G1134 gi3399777 0.00011 Glycine max symbiotic ammonium transporter; nodulin. 231 G1134 gi527665 0.00046 Sorghum bicolor myc-like regulatory R gene product. 231 G1134 gi13346182 0.0013 Gossypium hirsutum GHDEL65. 231 G1134 gi100921 0.0025 Zea mays regulatory protein B-Peru- maize. 233 G1140 AF346303 1.00E−68 Ipomoea batatas MADS box transcription factor (MADS4) mRNA, 233 G1140 AF335237 2.00E−62 Petunia x hybrida MADS-box transcription factor FBP13 (FBP1 233 G1140 BU837680 4.00E−62 Populus tremula x T104E08 Populus apica Populus tremuloides 233 G1140 AF008651 6.00E−61 Solanum tuberosum MADS transcriptional factor (Stmads16) mR 233 G1140 AB050643 2.00E−60 Magnolia praecocissima mRNA for putative MADS- domain transc 233 G1140 AF060880 5.00E−58 Paulownia kawakamii MADS box protein mRNA, complete cds. 233 G1140 AF144623 5.00E−58 Canavalia lineata MADS-box transcription factor (MADS) mRNA 233 G1140 AX403042 1.00E−57 Lycopersicon Sequence 3 from Patent esculentum WO0204651. 233 G1140 BU824503 2.00E−50 Populus tremula UB65DPB03 Populus tremula cambium cDNA libr 233 G1140 AY104901 7.00E−49 Zea mays PCO106306 mRNA sequence. 233 G1140 gi13448660 2.20E−66 Ipomoea batatas MADS box transcription factor. 233 G1140 gi13384052 1.40E−64 Petunia x hybrida MADS-box transcription factor FBP13. 233 G1140 gi2735764 5.00E−60 Solanum tuberosum MADS transcriptional factor; STMADS16. 233 G1140 gi17433048 2.10E−59 Lycopersicon MADS-box JOINTLESS esculentum protein (LeMAD 233 G1140 gi16549058 9.40E−59 Magnolia praecocissima putative MADS-domain transcription 233 G1140 gi6652756 8.50E−58 Paulownia kawakamii MADS box protein. 233 G1140 gi7672991 2.90E−57 Canavalia lineata MADS-box transcription factor. 233 G1140 gi5295978 1.40E−48 Oryza sativa MADS box-like protein. 233 G1140 gi9367234 1.10E−46 Hordeum vulgare MADS-box protein 1-2. 233 G1140 gi3986689 2.10E−45 Cichorium intybus MADS box protein. 235 G1143 BH962188 6.00E−31 Brassica oleracea odd86h08.b1 B. oleracea002 Brassica olerac 235 G1143 BI932387 2.00E−19 Lycopersicon EST552276 tomato flower, esculentum 8 mm to pr 235 G1143 AU288464 3.00E−14 Zinnia elegans AU288464 zinnia cultured mesophyll cell equa 235 G1143 BF004604 7.00E−11 Medicago truncatula EST433102 KV1 Medicago truncatula cDNA 235 G1143 PVU18348 2.00E−09 Phaseolus vulgaris phaseolin G-box binding protein PG1 (PG1 235 G1143 BQ505669 3.00E−09 Solanum tuberosum EST613084 Generation of a set of potato c 235 G1143 CA502087 1.00E−08 Triticum aestivum WHE4042_E12_I24ZT Wheat meiotic anther cD 235 G1143 BQ854856 2.00E−08 Lactuca sativa QGB24G11.yg.ab1 QG_ABCDI lettuce salinas Lac 235 G1143 BU763190 3.00E−08 Glycine max sas38f03.y1 Gm-c1080 Glycine max cDNA clone SOY 235 G1143 AF260919 3.00E−08 Petunia x hybrida anthocyanin 1 (an1) mRNA, an1-V26 allele, 235 G1143 gi1142619 1.20E−11 Phaseolus vulgaris phaseolin G-box binding protein PG1. 235 G1143 gi6175252 1.20E−09 Lycopersicon jasmonic acid 3. esculentum 235 G1143 gi7339702 1.40E−09 Oryza sativa EST AU065085(F11092) corresponds to a region 235 G1143 gi10998404 3.30E−09 Petunia x hybrida anthocyanin 1. 235 G1143 gi527655 3.80E−09 Pennisetum glaucum myc-like regulatory R gene product. 235 G1143 gi22758263 1.50E−08 Oryza sativa (japonica Putative bHLH tran cultivar-group) 235 G1143 gi3399777 1.90E−08 Glycine max symbiotic ammonium transporter; nodulin. 235 G1143 gi4321762 8.90E−08 Zea mays transcription factor MYC7E. 235 G1143 gi13346180 1.70E−07 Gossypium hirsutum GHDEL61. 235 G1143 gi527665 6.50E−07 Sorghum bicolor myc-like regulatory R gene product. 237 G1146 AB081950  1.0e−999 Oryza sativa (japonica ( ) OsPNH1 mRNA for cultivar-group) 237 G1146 AY109385  1.0e−999 Zea mays CL857_2 mRNA sequence. 237 G1146 BF269617 1.00E−149 Gossypium arboreum GA_Eb0005C21f Gossypium arboreum 7-10 d 237 G1146 BI118817 1.00E−146 Oryza sativa EST205 Differentially expressed cDNA libraries 237 G1146 AAAA01000124 1.00E−145 Oryza sativa (indica ( ) scaffold000124 cultivar-group) 237 G1146 BG648445 1.00E−138 Medicago truncatula EST510064 HOGA Medicago truncatula cDNA 237 G1146 BG351593 1.00E−135 Solanum tuberosum 129B03 Mature tuber lambda ZAPSolanum tu 237 G1146 BU894661 1.00E−131 Populus tremula x X012H09 Populus wood Populus tremuloides 237 G1146 BG125123 1.00E−126 Lycopersicon EST470769 tomato esculentum shoot/meristem Lyc 237 G1146 BF265852 1.00E−120 Hordeum vulgare HV_CEa0013I03f Hordeum vulgare seedling gre 237 G1146 gi21280321  1.0e−999 Oryza sativa (japonica ZLL/PNH homologous cultivar-group) 237 G1146 gi6539559 1.70E−103 Oryza sativa ESTs AU068544(C30430), C98487 (E0325), D23445(C 237 G1146 gi18542175 1.20E−54 Zea mays putative pinhead protein. 237 G1146 gi559557 0.02 Pyrus communis arabinogalactan-protein. 237 G1146 gi4103618 0.59 Fragaria x ananassa HyPRP. 237 G1146 gi6651027 0.66 Brassica napus high mobility group protein I/Y. 237 G1146 gi322757 0.86 Nicotiana tabacum pistil extensin-like protein (clone pMG 237 G1146 gi806720 0.86 Nicotiana alata arabinogalactan-protein precursor. 237 G1146 gi1076211 0.93 Chlamydomonas hypothetical protein VSP-3- reinhardtii Ch 237 G1146 gi6523547 0.94 Volvox carteri f. hydroxyproline-rich glycopr nagariensis 239 G1196 AX041006 1.00E−112 Zea mays Sequence 1 from Patent WO0065037. 239 G1196 AX351139 1.00E−106 Oryza sativa Sequence 13 from Patent WO0166755. 239 G1196 AX049431 1.00E−105 Triticum aestivum Sequence 6 from Patent WO0070069. 239 G1196 BH483537 7.00E−90 Brassica oleracea BOGXP26TF BOGX Brassica oleracea genomic 239 G1196 AF480488 5.00E−78 Nicotiana tabacum NPR1 mRNA, complete cds. 239 G1196 AAAA01000043 1.00E−68 Oryza sativa (indica ( ) scaffold000043 cultivar-group) 239 G1196 BM111027 6.00E−68 Solanum tuberosum EST558563 potato roots Solanum tuberosum 239 G1196 BQ849921 1.00E−67 Lactuca sativa QGB11C22.yg.ab1 QG_ABCDI lettuce salinas Lac 239 G1196 AF527176 9.00E−67 Brassica napus putative NPR1 (NPR1) mRNA, complete cds. 239 G1196 BQ148533 2.00E−65 Medicago truncatula NF069A11FL1F1085 Developing flower Medi 239 G1196 gi11340603 3.10E−118 Zea mays unnamed protein product. 239 G1196 gi22535593 3.50E−111 Oryza sativa (japonica putative Regulator cultivar-group) 239 G1196 gi18616497 3.50E−109 Triticum aestivum unnamed protein product. 239 G1196 gi18616493 1.10E−105 Oryza sativa unnamed protein product. 239 G1196 gi21552981 3.40E−77 Nicotiana tabacum NPR1. 239 G1196 gi22003730 3.30E−71 Brassica napus putative NPR1. 239 G1196 gi4433618 0.1 Dendrobium grex putative myosin heavy cha Madame Thong-In 239 G1196 gi17645766 0.71 Glycine max unnamed protein product. 239 G1196 gi421970 0.76 Helianthus annuus hypothetical protein 708- common sunfl 239 G1196 gi223934 0.9 Hordeum vulgare var. protein, acyl carrier. distichum 241 G1198 AF036949 1.00E−119 Zea mays basic leucine zipper protein (liguleless2) mRNA, c 241 G1198 BD016868 1.00E−100 Oryza sativa Rice-origin information transmission-related g 241 G1198 NTU90214 1.00E−100 Nicotiana tabacum leucine zipper transcription factor TGA2. 241 G1198 AF402608 1.00E−99 Phaseolus vulgaris TGA-type basic leucine zipper protein TG 241 G1198 AX180962 9.00E−99 Physcomitrella patens Sequence 13 from Patent WO0145493. 241 G1198 WHTHBP1BC1 5.00E−96 Triticum aestivum mRNA for transcription factor HBP-1b(c1 241 G1198 VFACREBL 1.00E−90 Vicia faba CREB-like protein mRNA, complete cds. 241 G1198 SOYSTGA 2.00E−84 Glycine max TGACG-motif binding protein (STGA1) mRNA, compl 241 G1198 BG645576 3.00E−82 Medicago truncatula EST507195 KV3 Medicago truncatula cDNA 241 G1198 NICTGA1A 3.00E−76 Nicotiana sp. Tobacco mRNA for TGA1a DNA-binding protein. 241 G1198 gi2865394 4.20E−115 Zea mays basic leucine zipper protein. 241 G1198 gi20161642 5.40E−96 Oryza sativa (japonica putative basic leu cultivar-group) 241 G1198 gi17025918 9.80E−96 Oryza sativa bZIP transcription factor. 241 G1198 gi12230709 3.30E−95 Nicotiana tabacum TGACG-SEQUENCE SPECIFIC DNA-BINDING PRO 241 G1198 gi15148924 4.20E−95 Phaseolus vulgaris TGA-type basic leucine zipper protein 241 G1198 gi1076782 1.00E−91 Triticum aestivum transcription factor HBP- 1b(c1)-wheat 241 G1198 gi7488719 1.60E−81 Glycine max transcription factor STGA1- soybean. 241 G1198 gi19680 6.60E−74 Nicotiana sp. TGA1a protein (AA 1-359). 241 G1198 gi100099 1.10E−73 Vicia faba DNA-binding protein VBP1- fava bean. 241 G1198 gi13195751 3.30E−72 Solanum tuberosum mas-binding factor MBF3. 243 G1225 BQ995023 4.00E−63 Lactuca sativa QGF8N12.yg.ab1 QG_EFGHJ lettuce serriola Lac 243 G1225 BH683493 7.00E−49 Brassica oleracea BOMIX45TF BO_2_3_KB Brassica oleracea gen 243 G1225 BI677665 3.00E−40 Robinia pseudoacacia CLS342 CLS (Cambium and bark region of 243 G1225 CA803022 2.00E−39 Glycine max sau46b03.y1 Gm-c1071 Glycine max cDNA clone SOY 243 G1225 BG590086 1.00E−34 Solanum tuberosum EST497928 P. infestans- challenged leaf So 243 G1225 AP004213 7.00E−31 Oryza sativa (japonica ( ) chromosome 8 clo cultivar-group) 243 G1225 BI310616 9.00E−31 Medicago truncatula EST5312366 GESD Medicago truncatula cDN 243 G1225 CAR011013 1.00E−30 Cicer arietinum epicotyl EST, clone Can133. 243 G1225 AAAA01002332 2.00E−29 Oryza sativa (indica ( ) scaffold002332 cultivar-group) 243 G1225 AC098836 9.00E−29 Oryza sativa chromosome 5 clone OJ2097B11, *** SEQUENCING I 243 G1225 gi24756878 4.50E−43 Oryza sativa (japonica Unknown protein. cultivar-group) 243 G1225 gi3641870 3.50E−20 Cicer arietinum hypothetical protein. 243 G1225 gi4321762 2.60E−10 Zea mays transcription factor MYC7E. 243 G1225 gi12643064 1.10E−09 Oryza sativa putative MYC transcription factor. 243 G1225 gi1142621 2.20E−09 Phaseolus vulgaris phaseolin G-box binding protein PG2. 243 G1225 gi527663 3.60E−08 Tripsacum australe myc-like regulatory R gene product. 243 G1225 gi527653 2.70E−07 Pennisetum glaucum myc-like regulatory R gene product. 243 G1225 gi1086526 3.40E−07 Oryza australiensis transcriptional activator Ra homolog. 243 G1225 gi1086528 4.80E−07 Oryza eichingeri transcriptional activator Ra homolog. 243 G1225 gi10998404 6.40E−07 Petunia x hybrida anthocyanin 1. 245 G1226 BH589494 1.00E−56 Brassica oleracea BOGIA17TR BOGI Brassica oleracea genomic 245 G1226 BQ995023 1.00E−43 Lactuca sativa QGF8N12.yg.ab1 QG_EFGHJ lettuce serriola Lac 245 G1226 BI677665 5.00E−42 Robinia pseudoacacia CLS342 CLS (Cambium and bark region of 245 G1226 BE021887 5.00E−36 Glycine max sm63g05.y1 Gm-c1028 Glycine max cDNA clone GENO 245 G1226 AP004213 1.00E−33 Oryza sativa (japonica ( ) chromosome 8 clo cultivar-group) 245 G1226 AAAA01002332 9.00E−33 Oryza sativa (indica ( ) scaffold002332 cultivar-group) 245 G1226 CAR011013 6.00E−32 Cicer arietinum epicotyl EST, clone Can133. 245 G1226 BI480474 5.00E−31 Triticum aestivum WHE2903_F02_L03ZS Wheat aluminum-stressed 245 G1226 BG452053 6.00E−28 Medicago truncatula NF077E11LF1F1087 Developing leaf Medica 245 G1226 BG590086 2.00E−27 Solanum tuberosum EST497928 P. infestans- challenged leaf So 245 G1226 gi19920107 2.20E−50 Oryza sativa (japonica Putative helix-loo cultivar-group) 245 G1226 gi3641870 5.30E−33 Cicer arietinum hypothetical protein. 245 G1226 gi1142621 4.90E−14 Phaseolus vulgaris phaseolin G-box binding protein PG2. 245 G1226 gi4321762 1.10E−11 Zea mays transcription factor MYC7E. 245 G1226 gi10998404 1.10E−10 Petunia x hybrida anthocyanin 1. 245 G1226 gi3399777 4.20E−10 Glycine max symbiotic ammonium transporter; nodulin. 245 G1226 gi12643064 2.00E−09 Oryza sativa putative MYC transcription factor. 245 G1226 gi6175252 5.10E−09 Lycopersicon jasmonic acid 3. esculentum 245 G1226 gi4206118 3.50E−08 Mesembryanthemum transporter homolog. crystallinum 245 G1226 gi527657 5.50E−08 Pennisetum glaucum myc-like regulatory R gene product. 247 G1229 BH473443 1.00E−96 Brassica oleracea BOHNJ20TR BOHN Brassica oleracea genomic 247 G1229 AAAA01009795 4.00E−38 Oryza sativa (indica ( ) scaffold009795 cultivar-group) 247 G1229 AP005470 6.00E−38 Oryza sativa (japonica ( ) chromosome 6 clo cultivar-group) 247 G1229 AP003978 7.00E−37 Oryza sativa chromosome 2 clone OJ1014_E11, *** SEQUENCING 247 G1229 BG590086 2.00E−20 Solanum tuberosum EST497928 P. infestans- challenged leaf So 247 G1229 BI310616 3.00E−20 Medicago truncatula EST5312366 GESD Medicago truncatula cDN 247 G1229 BG316255 6.00E−20 Glycine max sab78e02.y1 Gm-c1032 Glycine max cDNA clone GEN 247 G1229 BQ995023 4.00E−19 Lactuca sativa QGF8N12.yg.ab1 QG_EFGHJ lettuce serriola Lac 247 G1229 BE033916 2.00E−18 Mesembryanthemum MG02A08 MG crystallinum Mesembryanthemum c 247 G1229 BU820988 8.00E−17 Populus tremula UB17CPF03 Populus tremula cambium cDNA libr 247 G1229 gi24756878 3.30E−31 Oryza sativa (japonica Unknown protein. cultivar-group) 247 G1229 gi3641870 2.30E−21 Cicer arietinum hypothetical protein. 247 G1229 gi1142621 1.80E−12 Phaseolus vulgaris phaseolin G-box binding protein PG2. 247 G1229 gi1420924 3.90E−11 Zea mays IN1. 247 G1229 gi12643064 4.50E−10 Oryza sativa putative MYC transcription factor. 247 G1229 gi3399777 4.30E−09 Glycine max symbiotic ammonium transporter; nodulin. 247 G1229 gi10998404 1.90E−08 Petunia x hybrida anthocyanin 1. 247 G1229 gi527663 2.70E−08 Tripsacum australe myc-like regulatory R gene product. 247 G1229 gi1086526 7.20E−08 Oryza australiensis transcriptional activator Ra homolog. 247 G1229 gi527665 7.30E−08 Sorghum bicolor myc-like regulatory R gene product. 249 G1255 BZ003641 3.00E−71 Brassica oleracea oeh85a08.g1 B. oleracea002 Brassica olerac 249 G1255 AP004993 2.00E−67 Oryza sativa (japonica ( ) chromosome 6 clo cultivar-group) 249 G1255 AAAA01023497 5.00E−45 Oryza sativa (indica ( ) scaffold023497 cultivar-group) 249 G1255 BU007090 2.00E−37 Lactuca sativa QGH13F16.yg.ab1 QG_EFGHJ lettuce serriola La 249 G1255 AC087181 1.00E−36 Oryza sativa chromosome 3 clone OSJNBa0018H01, *** SEQUENCI 249 G1255 BG321336 7.00E−36 Descurainia sophia Ds01_06h10_A Ds01_AAFC_ECORC_cold_stress 249 G1255 BG239774 1.00E−34 Glycine max sab74c03.y1 Gm-c1032 Glycine max cDNA clone GEN 249 G1255 BQ139046 7.00E−33 Medicago truncatula NF010E05PH1F1036 Phoma-infected Medicag 249 G1255 BQ489587 1.00E−31 Beta vulgaris 50-E9232-006-008-C14-T3 Sugar beet MPIZ-ADIS- 249 G1255 AI772841 7.00E−31 Lycopersicon EST253941 tomato esculentum resistant, Cornell 249 G1255 gi13702811 7.80E−32 Oryza sativa putative zinc finger protein. 249 G1255 gi22854920 4.10E−22 Brassica nigra COL1 protein. 249 G1255 gi2895188 6.20E−21 Brassica napus CONSTANS homolog. 249 G1255 gi21667479 2.30E−19 Hordeum vulgare CONSTANS-like protein. 249 G1255 gi23589949 3.60E−19 Oryza sativa (japonica Hd1. cultivar-group) 249 G1255 gi4091804 4.00E−19 Malus x domestica CONSTANS-like protein 1. 249 G1255 gi21655168 4.40E−19 Hordeum vulgare subsp. CONSTANS-like protein vulgare CO8. 249 G1255 gi3341723 7.80E−19 Raphanus sativus CONSTANS-like 1 protein. 249 G1255 gi10946337 9.40E−18 Ipomoea nil CONSTANS-like protein. 249 G1255 gi4557093 6.00E−16 Pinus radiata zinc finger protein. 251 G1266 BH460596 2.00E−91 Brassica oleracea BOGWG80TR BOGW Brassica oleracea genomic 251 G1266 AF494201 1.00E−54 Lycopersicon transcription factor TSRF1 esculentum (TSRF1) 251 G1266 NTU81157 2.00E−53 Nicotiana tabacum S25-XP1 DNA binding protein mRNA, complet 251 G1266 BQ081329 8.00E−48 Glycine max san23a04.y1 Gm-c1084 Glycine max cDNA clone SOY 251 G1266 BG449954 8.00E−45 Medicago truncatula NF013A10DT1F1081 Drought Medicago trunc 251 G1266 BU896285 3.00E−43 Populus tremula x X038D06 Populus wood Populus tremuloides 251 G1266 AI967551 9.00E−39 Lotus japonicus Ljirnpest05-400-d11 Ljirnp Lambda HybriZap 251 G1266 AI055252 6.00E−36 Gossypium hirsutum coau0003H16 Cotton Boll Abscission Zone 251 G1266 AAAA01000537 9.00E−36 Oryza sativa (indica ( ) scaffold000537 cultivar-group) 251 G1266 AC092263 9.00E−36 Oryza sativa chromosome 3 clone OSJNBa0033P04, *** SEQUENCI 251 G1266 gi23452024 2.10E−54 Lycopersicon transcription factor TSRF1. esculentum 251 G1266 gi1732406 1.00E−52 Nicotiana tabacum S25-XP1 DNA binding protein. 251 G1266 gi19034045 8.10E−37 Oryza sativa (japonica putative DNA bindi cultivar-group) 251 G1266 gi7528276 4.70E−29 Mesembryanthemum AP2-related transcription f crystallinum 251 G1266 gi8809571 1.20E−26 Nicotiana sylvestris ethylene-responsive element binding 251 G1266 gi17385636 1.80E−25 Matricaria chamomilla ethylene-responsive element binding 251 G1266 gi8346775 1.00E−23 Catharanthus roseus AP2-domain DNA-binding protein. 251 G1266 gi14140141 2.30E−23 Oryza sativa putative AP2-related transcription factor. 251 G1266 gi21304712 1.30E−20 Glycine max ethylene-responsive element binding protein 1 251 G1266 gi24817250 4.30E−18 Cicer arietinum transcription factor EREBP- like protein. 253 G1275 AF056948 9.00E−33 Gossypium hirsutum AF056948 Cotton drought tolerant genotyp 253 G1275 BQ984602 2.00E−32 Lactuca sativa QGE3d01.yg.ab1 QG_EFGHJ lettuce serriola Lac 253 G1275 BE216050 7.00E−32 Hordeum vulgare HV_CEb0009E04f Hordeum vulgare seedling gre 253 G1275 AW565483 3.00E−31 Sorghum bicolor LG1_344_C03.g1_A002 Light Grown 1 (LG1) Sor 253 G1275 BM064330 4.00E−31 Capsicum annuum KS01065H01 KS01 Capsicum annuum cDNA, mRNA 253 G1275 BM334368 6.00E−31 Zea mays MEST136-B12.T3 ISUM5- RN Zea mays cDNA clone MEST13 253 G1275 BG525040 6.00E−31 Stevia rebaudiana 46-57 Stevia field grown leaf cDNA Stevia 253 G1275 BE230596 1.00E−30 Oryza sativa 99AS81 Rice Seedling Lambda ZAPII cDNA Library 253 G1275 BF009428 2.00E−30 Glycine max ss78f04.y1 Gm-c1064 Glycine max cDNA clone GENO 253 G1275 BJ449458 2.00E−30 Hordeum vulgare subsp. BJ449458 K. Sato vulgare unpublished 253 G1275 gi14588677 4.80E−31 Oryza sativa hypothetical protein. 253 G1275 gi21644680 4.80E−31 Oryza sativa (japonica hypothetical prote cultivar-group) 253 G1275 gi4894965 6.10E−24 Avena sativa DNA-binding protein WRKY1. 253 G1275 gi14530683 2.30E−23 Nicotiana tabacum WRKY DNA-binding protein. 253 G1275 gi1432056 3.80E−23 Petroselinum crispum WRKY3. 253 G1275 gi18158619 5.40E−23 Retama raetam WRKY-like drought- induced protein. 253 G1275 gi24745606 7.90E−23 Solanum tuberosum WRKY-type DNA binding protein. 253 G1275 gi1076685 3.60E−22 Ipomoea batatas SPF1 protein-sweet potato. 253 G1275 gi23305051 4.00E−22 Oryza sativa (indica WRKY transcription f cultivar-group) 253 G1275 gi1159877 6.00E−22 Avena fatua DNA-binding protein. 255 G1305 AW685439 9.00E−51 Medicago truncatula NF029D11NR1F1000 Nodulated root Medicag 255 G1305 AB028649 6.00E−50 Nicotiana tabacum gene for myb-related transcription factor 255 G1305 PHMYBPH22 1.00E−48 Petunia x hybrida P. Hybrida myb.Ph2 gene encoding protein 255 G1305 AB073016 1.00E−48 Vitis labrusca x Vitis VlmybB1-1 gene for myb- vinifera rela 255 G1305 AB029160 4.00E−48 Glycine max gene for GmMYB291, complete cds. 255 G1305 BQ514539 6.00E−47 Solanum tuberosum EST621954 Generation of a set of potato c 255 G1305 AW032652 8.00E−47 Lycopersicon EST276211 tomato callus, esculentum TAMU Lycop 255 G1305 OSMYB1202 1.00E−46 Oryza sativa O. sativa mRNA for myb factor, 1202 bp. 255 G1305 BF201950 2.00E−45 Triticum aestivum WHE1759- 1762_N04_N04ZS Wheat pre-anthesis 255 G1305 AP004786 2.00E−44 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 255 G1305 gi10140742 5.70E−51 Oryza sativa myb factor. 255 G1305 gi20561 2.30E−50 Petunia x hybrida protein 2. 255 G1305 gi5139814 3.70E−50 Glycine max GmMYB29B2. 255 G1305 gi6552359 2.50E−49 Nicotiana tabacum myb-related transcription factor LBM1. 255 G1305 gi22266673 3.70E−48 Vitis labrusca x Vitis myb-related transcription vinifera 255 G1305 gi127580 8.90E−47 Zea mays MYB-RELATED PROTEIN ZM1. 255 G1305 gi1370140 1.80E−46 Lycopersicon myb-related transcription esculentum factor. 255 G1305 gi19548405 1.20E−44 Sorghum bicolor P-type R2R3 Myb protein. 255 G1305 gi82308 8.20E−44 Antirrhinum majus myb protein 308-garden snapdragon. 255 G1305 gi13346194 1.70E−43 Gossypium hirsutum GHMYB9. 257 G1322 AI486576 4.00E−59 Lycopersicon EST244897 tomato ovary, esculentum TAMU Lycope 257 G1322 PSMYB26 2.00E−58 Pisum sativum P. sativum mRNA for Myb- like protein (Myb26). 257 G1322 BG457971 1.00E−55 Medicago truncatula NF037A10PL1F1070 Phosphate starved leaf 257 G1322 BM528383 9.00E−54 Glycine max sa157f09.y1 Gm-c1061 Glycine max cDNA clone SOY 257 G1322 BI978095 1.00E−53 Rosa chinensis pE09 Old Blush petal SMART library Rosa chin 257 G1322 BQ106505 6.00E−53 Rosa hybrid cultivar fc0568.e Rose Petals (Fragrant Cloud) 257 G1322 BQ584246 1.00E−51 Beta vulgaris E011860-024-003-F21-SP6 MPIZ-ADIS-024-inflore 257 G1322 BU867210 5.00E−48 Populus tremula x S075F04 Populus imbib Populus tremuloides 257 G1322 AB058642 6.00E−48 Lilium hybrid division I LhMyb mRNA, complete cds. 257 G1322 CPU33917 7.00E−47 Craterostigma myb-related transcription plantagineum factor 257 G1322 gi82306 2.90E−57 Antirrhinum majus myb protein 305-garden snapdragon. 257 G1322 gi1841475 2.10E−52 Pisum sativum Myb26. 257 G1322 gi1002796 5.40E−51 Craterostigma Cpm10. plantagineum 257 G1322 gi13537530 4.70E−48 Lilium hybrid division I LhMyb. 257 G1322 gi13177578 2.00E−47 Oryza sativa Myb transcription factor JAMyb. 257 G1322 gi23476307 2.90E−46 Gossypioides kirkii myb-like transcription factor 5. 257 G1322 gi14249015 4.70E−46 Gossypium hirsutum myb-like transcription factor Myb 5. 257 G1322 gi23476303 4.70E−46 Gossypium raimondii myb-like transcription factor 2. 257 G1322 gi24059885 6.20E−46 Oryza sativa (japonica putative typical P cultivar-group) 257 G1322 gi19073328 8.00E−46 Sorghum bicolor typical P-type R2R3 Myb protein. 259 G1323 BF644773 8.00E−54 Medicago truncatula NF020H12EC1F1103 Elicited cell culture 259 G1323 OSMYB1202 2.00E−53 Oryza sativa O. sativa mRNA for myb factor, 1202 bp. 259 G1323 AP004786 3.00E−53 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 259 G1323 AB028650 5.00E−53 Nicotiana tabacum mRNA for myb-related transcription factor 259 G1323 AAAA01006126 4.00E−52 Oryza sativa (indica ( ) scaffold006126 cultivar-group) 259 G1323 BF201950 7.00E−52 Triticum aestivum WHE1759- 1762_N04_N04ZS Wheat pre-anthesis 259 G1323 BG343209 100E−51 Hordeum vulgare HVSMEg0005B14f Hordeum vulgare pre- anthesis 259 G1323 CA032540 100E−51 Hordeum vulgare subsp. HX13G05r HX Hordeum vulgare vulgare 259 G1323 PHMYBPH22 100E−51 Petunia x hybrida P. Hybrida myb.Ph2 gene encoding protein 259 G1323 AB029160 2.00E−51 Glycine max gene for GmMYB291, complete cds. 259 G1323 gi6552361 1.30E−52 Nicotiana tabacum myb-related transcription factor LBM2. 259 G1323 gi1946265 3.50E−52 Oryza sativa myb. 259 G1323 gi5139802 4.00E−51 Glycine max GmMYB29A1. 259 G1323 gi22266673 1.10E−50 Vitis labrusca x Vitis myb-related transcription vinifera 259 G1323 gi1370140 1.40E−50 Lycopersicon myb-related transcription esculentum factor. 259 G1323 gi20561 2.80E−50 Petunia x hybrida protein 2. 259 G1323 gi127580 9.60E−50 Zea mays MYB-RELATED PROTEIN ZM1. 259 G1323 gi19548405 1.20E−49 Sorghum bicolor P-type R2R3 Myb protein. 259 G1323 gi22795039 7.70E−48 Populus x canescens putative MYB transcription factor. 259 G1323 gi4886264 2.30E−46 Antirrhinum majus Myb-related transcription factor mixta- 261 G1330 BU867210 5.00E−76 Populus tremula x S075F04 Populus imbib Populus tremuloides 261 G1330 BQ583496 3.00E−75 Beta vulgaris E011979-024-005-N01-SP6 MPIZ-ADIS-024-inflore 261 G1330 AF510112 1.00E−74 Craterostigma MYB transcription factor plantagineum (MYB10) 261 G1330 AW032656 1.00E−73 Lycopersicon EST276215 tomato callus, esculentum TAMU Lycop 261 G1330 AY026332 8.00E−71 Oryza sativa Myb transcription factor JAMyb mRNA, complete 261 G1330 AF034133 2.00E−68 Gossypium hirsutum MYB-like DNA-binding domain protein (Cmy 261 G1330 BJ233398 7.00E−67 Triticum aestivum BJ233398 Y. Ogihara unpublished cDNA libr 261 G1330 BG607379 5.00E−66 Triticum monococcum WHE2471_H10_O19ZS Triticum monococcum e 261 G1330 AAAA01002218 5.00E−65 Oryza sativa (indica ( ) scaffold002218 cultivar-group) 261 G1330 BF325282 1.00E−64 Glycine max su20e03.y1 Gm-c1066 Glycine max cDNA clone GENO 261 G1330 gi1002798 1.60E−70 Craterostigma Cpm5. plantagineum 261 G1330 gi14249015 1.50E−69 Gossypium hirsutum myb-like transcription factor Myb 5. 261 G1330 gi13177578 6.30E−69 Oryza sativa Myb transcription factor JAMyb. 261 G1330 gi23476303 1.30E−68 Gossypium raimondii myb-like transcription factor 2. 261 G1330 gi23476307 1.70E−68 Gossypioides kirkii myb-like transcription factor 5. 261 G1330 gi23476305 5.70E−68 Gossypium herbaceum myb-like transcription factor 5. 261 G1330 gi19073328 1.50E−67 Sorghum bicolor typical P-type R2R3 Myb protein. 261 G1330 gi24059885 4.60E−66 Oryza sativa (japonica putative typical P cultivar-group) 261 G1330 gi14970950 2.60E−63 Arabis gemmifera MYB transcription factor Atmyb2. 261 G1330 gi14970952 9.90E−54 Crucihimalaya MYB transcription factor himalaica Atmyb2. 263 G1331 BF644787 1.00E−65 Medicago truncatula NF016A03EC1F1020 Elicited cell culture 263 G1331 BH663145 1.00E−48 Brassica oleracea BOMIM96TR BO_2_3_KB Brassica oleracea gen 263 G1331 BE489186 4.00E−47 Triticum aestivum WHE1075_G04_M07ZS Wheat unstressed seedli 263 G1331 PSMYB26 1.00E−44 Pisum sativum P. sativum mRNA for Myb- like protein (Myb26). 263 G1331 BM527606 5.00E−43 Glycine max sa163g06.y1 Gm-c1061 Glycine max cDNA clone SOY 263 G1331 BU013207 6.00E−43 Lactuca sativa QGJ4A09.yg.ab1 QG_EFGHJ lettuce serriola Lac 263 G1331 BU991693 2.00E−42 Hordeum vulgare HD07K18r HD Hordeum vulgare cDNA clone HD07 263 G1331 BQ460434 2.00E−42 Hordeum vulgare subsp. HA09K10r HA Hordeum vulgare vulgare 263 G1331 BQ106505 5.00E−42 Rosa hybrid cultivar fc0568.e Rose Petals (Fragrant Cloud) 263 G1331 AI486576 5.00E−42 Lycopersicon EST244897 tomato ovary, esculentum TAMU Lycope 263 G1331 gi1841475 2.80E−43 Pisum sativum Myb26. 263 G1331 gi19073328 3.60E−43 Sorghum bicolor typical P-type R2R3 Myb protein. 263 G1331 gi11275531 6.60E−42 Oryza sativa putative myb-related transcription factor. 263 G1331 gi82306 1.10E−41 Antirrhinum majus myb protein 305-garden snapdragon. 263 G1331 gi24059885 1.80E−41 Oryza sativa (japonica putative typical P cultivar-group) 263 G1331 gi2921338 1.80E−41 Gossypium hirsutum MYB-like DNA-binding domain protein. 263 G1331 gi1167486 2.50E−41 Lycopersicon transcription factor. esculentum 263 G1331 gi23476303 3.70E−41 Gossypium raimondii myb-like transcription factor 2. 263 G1331 gi13537530 7.60E−41 Lilium hybrid division I LhMyb. 263 G1331 gi1002796 9.70E−41 Craterostigma Cpm10. plantagineum 265 G1332 AF122054 5.00E−49 Solanum tuberosum clone 9 tuber-specific and sucrose-respon 265 G1332 AW186273 2.00E−41 Glycine max se65f12.y1 Gm-c1019 Glycine max cDNA clone GENO 265 G1332 AF336282 2.00E−41 Gossypium hirsutum GHMYB10 (ghmyb10) mRNA, complete cds. 265 G1332 AF502295 4.00E−41 Cucumis sativus werewolf (WER) mRNA, partial cds. 265 G1332 BG441912 7.00E−41 Gossypium arboreum GA_Ea0015B19f Gossypium arboreum 7-10 d 265 G1332 BU891795 2.00E−40 Populus tremula P055C08 Populus petioles cDNA library Popul 265 G1332 OSC1ACTIV 3.00E−40 Oryza sativa subsp. Oryza sativa mRNA for indica transcrip 265 G1332 AY135019 1.00E−39 Zea mays PL transcription factor (pl) mRNA, pl-W22 allele, 265 G1332 BU827658 1.00E−39 Populus tremula x K006P59P Populus apic Populus tremuloides 265 G1332 AW065119 2.00E−39 Pinus taeda ST39H05 Pine TriplEx shoot tip library Pinus ta 265 G1332 gi9954118 6.70E−49 Solanum tuberosum tuber-specific and sucrose- responsive e 265 G1332 gi13346186 1.40E−41 Gossypium hirsutum GHMYB10. 265 G1332 gi20514371 3.70E−41 Cucumis sativus werewolf. 265 G1332 gi309572 9.70E−41 Zea mays transcriptional activator. 265 G1332 gi4138299 1.60E−40 Oryza sativa subsp. transcriptional activator. indica 265 G1332 gi23476297 3.30E−40 Gossypioides kirkii myb-like transcription factor 3. 265 G1332 gi14269333 5.40E−40 Gossypium raimondii myb-like transcription factor Myb 3. 265 G1332 gi1101770 1.00E−38 Picea mariana MYB-like transcriptional factor MBF1. 265 G1332 gi23476293 1.60E−38 Gossypium herbaceum myb-like transcription factor 2. 265 G1332 gi15042120 2.10E−38 Zea luxurians CI protein. 267 G1363 BH963585 1.00E−47 Brassica oleracea odd44e06.g1 B. oleracea002 Brassica olerac 267 G1363 AY109469 3.00E−36 Zea mays CL724_1 mRNA sequence. 267 G1363 OSRAPB 2.00E−34 Oryza sativa mRNA RAPB protein. 267 G1363 BU083572 1.00E−32 Glycine max sar22h11.y1 Gm-c1049 Glycine max cDNA clone SOY 267 G1363 CA794711 2.00E−32 Theobroma cacao Cac_BL_1066 Cac_BL (Bean and Leaf from Amel 267 G1363 BU987613 5.00E−30 Hordeum vulgare subsp. HF15E04r HF Hordeum vulgare vulgare 267 G1363 BU672328 8.00E−29 Triticum aestivum WHE3303_C07_F13ZS Chinese Spring wheat dr 267 G1363 BQ507104 1.00E−28 Solanum tuberosum EST614519 Generation of a set of potato c 267 G1363 BG457624 4.00E−28 Medicago truncatula NF104F12PL1F1101 Phosphate starved leaf 267 G1363 BJ479271 5.00E−28 Hordeum vulgare subsp. BJ479271 K. Sato unpublis spontaneum 267 G1363 gi2826786 8.10E−37 Oryza sativa RAPB protein. 267 G1363 gi7141243 8.50E−26 Vitis riparia transcription factor. 267 G1363 gi4731314 7.10E−22 Nicotiana tabacum CCAAT-binding transcription factor subu 267 G1363 gi1173616 9.00E−22 Brassica napus CCAAT-binding factor B subunit homolog. 267 G1363 gi24414083 0.43 Oryza sativa (japonica gag-pol-like prote cultivar-group) 267 G1363 gi4902535 0.57 Gossypium sturtianum microsomal omega6 desaturase enzyme. 267 G1363 gi15187138 0.75 Gossypium anomalum microsomal omega6 desaturase FAD2-1. 267 G1363 gi4902504 0.75 Gossypium microsomal omega6 cunninghamii desaturase enzym 267 G1363 gi4902502 0.83 Gossypium costulatum microsomal omega6 desaturase enzyme. 267 G1363 gi4902506 0.83 Gossypium enthyle microsomal omega6 desaturase enzyme. 269 G1411 BZ017225 3.00E−51 Brassica oleracea oei67e03.b1 B. oleracea002 Brassica olerac 269 G1411 BQ138607 8.00E−44 Medicago truncatula NF005C01PH1F1004 Phoma-infected Medicag 269 G1411 BQ786702 5.00E−36 Glycine max saq72b07.y1 Gm-c1076 Glycine max cDNA clone SOY 269 G1411 BM062508 7.00E−32 Capsicum annuum KS01043F09 KS01 Capsicum annuum cDNA, mRNA 269 G1411 AAAA01000832 2.00E−30 Oryza sativa (indica ( ) scaffold000832 cultivar-group) 269 G1411 OSJN00240 2.00E−30 Oryza sativa genomic DNA, chromosome 4, BAC clone: OSJNBa0 269 G1411 BE419451 2.00E−29 Triticum aestivum WWS012.C2R000101 ITEC WWS Wheat Scutellum 269 G1411 CA014817 6.00E−29 Hordeum vulgare subsp. HT12H01r HT Hordeum vulgare vulgare 269 G1411 BE642320 1.00E−28 Ceratopteris richardii Cri2_5_L17_SP6 Ceratopteris Spore Li 269 G1411 BE494041 2.00E−27 Secale cereale WHE1277_B09_D17ZS Secale cereale anther cDNA 269 G1411 gi20160854 1.40E−29 Oryza sativa (japonica hypothetical prote cultivar-group) 269 G1411 gi14140141 1.50E−24 Oryza sativa putative AP2-related transcription factor. 269 G1411 gi3342211 1.40E−23 Lycopersicon Pti4. esculentum 269 G1411 gi10798644 2.30E−23 Nicotiana tabacum AP2 domain-containing transcription fac 269 G1411 gi8809571 2.30E−23 Nicotiana sylvestris ethylene-responsive element binding 269 G1411 gi24817250 3.00E−23 Cicer arietinum transcription factor EREBP- like protein. 269 G1411 gi3264767 3.00E−23 Prunus armeniaca AP2 domain containing protein. 269 G1411 gi1688233 3.80E−23 Solanum tuberosum DNA binding protein homolog. 269 G1411 gi7528276 3.80E−23 Mesembryanthemum AP2-related transcription f crystallinum 269 G1411 gi21304712 6.20E−23 Glycine max ethylene-responsive element binding protein 1 271 G1417 CA782643 8.00E−58 Glycine max sat31e05.y1 Gm-c1056 Glycine max cDNA clone SOY 271 G1417 A1895084 9.00E−57 Lycopersicon EST264527 tomato callus, esculentum TAMU Lycop 271 G1417 BQ625082 3.00E−56 Citrus sinensis USDA-FP_02173 Ridge pineapple sweet orange 271 G1417 AC120986 2.00E−54 Oryza sativa (japonica ( ) chromosome 5 clo cultivar-group) 271 G1417 AAAA01004053 3.00E−54 Oryza sativa (indica ( ) scaffold004053 cultivar-group) 271 G1417 BF636342 2.00E−53 Medicago truncatula NF088G12DT1F1099 Drought Medicago trunc 271 G1417 BG838724 3.00E−50 Glycine clandestina Gc02_02f10_R Gc02_AAFC_ECORC_cold_stres 271 G1417 AU083645 2.00E−47 Cryptomeria japonica AU083645 Cryptomeria japonica inner ba 271 G1417 AP004967 6.00E−47 Lotus japonicus genomic DNA, chromosome 1, clone: LjT27L02, 271 G1417 BU047549 1.00E−46 Prunus persica PP_LEa0030E11f Peach developing fruit mesoca 271 G1417 gi8467950 4.80E−68 Oryza sativa Similar to Arabidopsis thaliana chromosome 4 271 G1417 gi20160973 2.40E−37 Oryza sativa (japonica hypothetical prote cultivar-group) 271 G1417 gi6472585 7.70E−36 Nicotiana tabacum WIZZ. 271 G1417 gi1159879 5.00E−35 Avena fatua DNA-binding protein. 271 G1417 gi1193822 2.50E−30 Petroselinum crispum transcription factor WRKY4. 271 G1417 gi3420906 1.80E−21 Pimpinella brachycarpa zinc finger protein; WRKY1. 271 G1417 gi4894965 4.90E−20 Avena sativa DNA-binding protein WRKY1. 271 G1417 gi18158619 2.80E−19 Retama raetam WRKY-like drought- induced protein. 271 G1417 gi1076685 3.60E−19 Ipomoea batatas SPF1 protein-sweet potato. 271 G1417 gi13620227 1.50E−18 Lycopersicon hypothetical protein. esculentum 273 G1419 TOBBY4C 6.00E−44 Nicotiana tabacum Tobacco mRNA for EREBP-4, complete cds. 273 G1419 BU823955 5.00E−43 Populus tremula UB58DPE07 Populus tremula cambium cDNA libr 273 G1419 AB016266 2.00E−42 Nicotiana sylvestris nserf4 gene for ethylene- responsive el 273 G1419 BM062245 5.00E−42 Capsicum annuum KS01040C11 KS01 Capsicum annuum cDNA, mRNA 273 G1419 AW507860 5.00E−40 Glycine max si45h05.y1 Gm-r1030 Glycine max cDNA clone GENO 273 G1419 BG646774 5.00E−39 Medicago truncatula EST508393 HOGA Medicago truncatula cDNA 273 G1419 AF204784 2.00E−38 Lycopersicon ripening regulated protein esculentum DDTFR10/ 273 G1419 BQ514195 3.00E−38 Solanum tuberosum EST621610 Generation of a set of potato c 273 G1419 CA812903 8.00E−35 Vitis vinifera CA48LU07IVF-D6 CA48LU Vitis vinifera cDNA cl 273 G1419 BH683728 8.00E−35 Brassica oleracea BOHTE23TR BO_2_3_KB Brassica oleracea gen 273 G1419 gi1208497 1.40E−48 Nicotiana tabacum EREBP-4. 273 G1419 gi8809575 9.80E−48 Nicotiana sylvestris ethylene-responsive element binding 273 G1419 gi12231294 3.00E−39 Lycopersicon ripening regulated protein esculentum DDTFR1 273 G1419 gi7528276 1.60E−30 Mesembryanthemum AP2-related transcription f crystallinum 273 G1419 gi12597874 6.80E−30 Oryza sativa putative ethylene-responsive element binding 273 G1419 gi17385636 3.30E−24 Matricaria chamomilla ethylene-responsive element binding 273 G1419 gi8980313 2.10E−23 Catharanthus roseus AP2-domain DNA-binding protein. 273 G1419 gi15623863 3.00E−23 Oryza sativa (japonica contains EST~hypot cultivar-group) 273 G1419 gi21304712 7.90E−23 Glycine max ethylene-responsive element binding protein 1 273 G1419 gi4099914 1.50E−21 Stylosanthes hamata ethylene-responsive element binding p 275 G1449 BH939388 8.00E−43 Brassica oleracea odd83a03.g1 B. oleracea002 Brassica olerac 275 G1449 BU927008 3.00E−34 Glycine max sas94e06.y1 Gm-c1036 Glycine max cDNA clone SOY 275 G1449 PTR306827 5.00E−30 Populus tremula x mRNA for aux/IAA pro Populus tremuloides 275 G1449 BF727992 3.00E−26 Zea mays 1000057B09.x4 1000- Unigene I from Maize Genome P 275 G1449 BF649039 3.00E−22 Medicago truncatula NF051G11EC1F1086 Elicited cell culture 275 G1449 BJ228821 3.00E−20 Triticum aestivum BJ228821 Y. Ogihara unpublished cDNA libr 275 G1449 AB026823 6.00E−20 Cucumis sativus CS-IAA3 mRNA, partial cds. 275 G1449 AB004933 2.00E−19 Vigna radiata mRNA for Aux22e, complete cds. 275 G1449 BU992079 2.00E−19 Hordeum vulgare HD08M04r HD Hordeum vulgare cDNA clone HD08 275 G1449 BU889599 3.00E−19 Populus tremula P023B06 Populus petioles cDNA library Popul 275 G1449 gi20269055 2.60E−31 Populus tremula x aux/IAA protein. Populus tremuloides 275 G1449 gi8096369 4.50E−27 Oryza sativa ESTs D22686(C0916), C98167(C0 916) correspond 275 G1449 gi6136834 1.30E−22 Cucumis sativus CS-IAA3. 275 G1449 gi4887022 7.60E−22 Nicotiana tabacum Nt-iaa4.1 deduced protein. 275 G1449 gi11131105 3.90E−21 Vigna radiata AUXIN-INDUCED PROTEIN 22E (INDOLE-3- ACETIC 275 G1449 gi1352057 1.00E−20 Pisum sativum AUXIN-INDUCED PROTEIN IAA4. 275 G1449 gi18071490 1.30E−20 Antirrhinum majus auxin-induced AUX/IAA1. 275 G1449 gi17976835 1.20E−19 Pinus pinaster putative auxin induced transcription facto 275 G1449 gi2388689 1.50E−19 Glycine max GH1 protein. 275 G1449 gi20257219 2.50E−19 Zinnia elegans auxin-regulated protein. 277 G1451 AB071298  1.0e−999 Oryza sativa OsARF8 mRNA for auxin response factor 8, parti 277 G1451 AY105215 1.00E−157 Zea mays PCO121637 mRNA sequence. 277 G1451 AW690130 1.00E−109 Medicago truncatula NF028B12ST1F1000 Developing stem Medica 277 G1451 BQ862285 1.00E−108 Lactuca sativa QGC20K23.yg.ab1 QG_ABCDI lettuce salinas Lac 277 G1451 BG597435 1.00E−107 Solanum tuberosum EST496113 cSTS Solanum tuberosum cDNA clo 277 G1451 BJ303602 1.00E−104 Triticum aestivum BJ303602 Y. Ogihara unpublished cDNA libr 277 G1451 OSA306306 1.00E−103 Oryza sativa (japonica Oryza sativa subsp. cultivar-group) 277 G1451 BQ595269 1.00E−89 Beta vulgaris E012710-024-023-D13-SP6 MPIZ-ADIS-024-develop 277 G1451 CA801218 1.00E−86 Glycine max sau02f06.y2 Gm-c1062 Glycine max cDNA clone SOY 277 G1451 BG159611 8.00E−79 Sorghum bicolor OV2_6_G07.b1_A002 Ovary 2 (OV2) Sorghum bic 277 G1451 gi19352049 3.70E−247 Oryza sativa auxin response factor 8. 277 G1451 gi20805236 3.10E−126 Oryza sativa (japonica auxin response fac cultivar-group) 277 G1451 gi24785191 4.10E−55 Nicotiana tabacum hypothetical protein. 277 G1451 gi23343944 2.40E−28 Mirabilis jalapa auxin-responsive factor protein. 277 G1451 gi20269053 7.00E−10 Populus tremula x aux/IAA protein. Populus tremuloides 277 G1451 gi287566 3.10E−06 Vigna radiata ORF. 277 G1451 gi114733 1.10E−05 Glycine max AUXIN-INDUCED PROTEIN AUX22. 277 G1451 gi871511 2.40E−05 Pisum sativum auxin-induced protein 277 G1451 gi18697008 0.00027 Zea mays unnamed protein product. 277 G1451 gi17976835 0.00068 Pinus pinaster putative auxin induced transcription facto 279 G1452 BF645605 4.00E−65 Medicago truncatula NF017A10EC1F1072 Elicited cell culture 279 G1452 BI140703 5.00E−43 Sorghum bicolor IP1_52_F12.b1_A002 Immature pannicle 1 (IP1 279 G1452 BQ469035 9.00E−43 Hordeum vulgare HM03C20r HM Hordeum vulgare cDNA clone HM03 279 G1452 BU967516 9.00E−43 Hordeum vulgare subsp. HB04I23r BC Hordeum vulgare vulgare 279 G1452 BJ481205 9.00E−43 Hordeum vulgare subsp. BJ481205 K. Sato unpublis spontaneum 279 G1452 BQ620568 2.00E−42 Triticum aestivum TaLr1142G07R TaLr1 Triticum aestivum cDNA 279 G1452 AB028187 8.00E−42 Oryza sativa mRNA for OsNAC8 protein, complete cds. 279 G1452 BQ997138 3.00E−41 Lactuca sativa QGG14N12.yg.ab1 QG_EFGHJ lettuce serriola La 279 G1452 BG543974 4.00E−40 Brassica rapa subsp. E1725 Chinese cabbage pekinensis etiol 279 G1452 AF509874 4.00E−40 Petunia x hybrida nam-like protein 11 (NH11) mRNA, complete 279 G1452 gi6730946 1.10E−44 Oryza sativa OsNAC8 protein. 279 G1452 gi21105746 9.50E−42 Petunia x hybrida nam-like protein 9. 279 G1452 gi7716952 4.70E−41 Medicago truncatula NAC1. 279 G1452 gi19225018 6.00E−41 Oryza sativa (japonica putative NAM (no a cultivar-group) 279 G1452 gi22597158 4.30E−38 Glycine max no apical meristem-like protein. 279 G1452 gi15148914 5.70E−36 Phaseolus vulgaris NAC domain protein NAC2. 279 G1452 gi4218537 3.20E−35 Triticum sp. GRAB2 protein. 279 G1452 gi6732160 3.20E−35 Triticum monococcum unnamed protein product. 279 G1452 gi6175246 5.90E−34 Lycopersicon jasmonic acid 2. esculentum 279 G1452 gi14485513 2.00E−33 Solanum tuberosum putative NAC domain protein. 281 G1463 BH478066 2.00E−72 Brassica oleracea BOHQV38TR BOHQ Brassica oleracea genomic 281 G1463 BE461560 1.00E−05 Lycopersicon EST412979 tomato breaker esculentum fruit, TIG 281 G1463 AAAA01002994 1.00E−05 Oryza sativa (indica ( ) scaffold002994 cultivar-group) 281 G1463 AP005621 1.00E−05 Oryza sativa (japonica ( ) chromosome 6 clo cultivar-group) 281 G1463 OSJN01006 1.00E−05 Oryza sativa chromosome x clone OSJNBa0082A03, *** SEQUENC 281 G1463 BQ852361 7.00E−05 Lactuca sativa QGB17N02.yg.ab1 QG_ABCDI lettuce salinas Lac 281 G1463 BG440924 3.00E−04 Gossypium arboreum GA_Ea0010P20f Gossypium arboreum 7-10 d 281 G1463 BU763436 4.00E−04 Glycine max sas42e12.y1 Gm-c1080 Glycine max cDNA clone SOY 281 G1463 BM406262 5.00E−04 Solanum tuberosum EST580589 potato roots Solanum tuberosum 281 G1463 AI729055 0.002 Gossypium hirsutum BNLGHi12472 Six-day Cotton fiber Gossypi 281 G1463 gi13129497 1.40E−07 Oryza sativa putative NAM (no apical meristem) protein. 281 G1463 gi21389176 1.30E−06 Petunia x hybrida nam-like protein 19. 281 G1463 gi22002150 7.70E−05 Oryza sativa (japonica putative NAM (no a cultivar-group) 281 G1463 gi6175246 0.00012 Lycopersicon jasmonic acid 2. esculentum 281 G1463 gi22597158 0.00056 Glycine max no apical meristem-like protein. 281 G1463 gi6732156 0.013 Triticum monococcum unnamed protein product. 281 G1463 gi15148912 0.02 Phaseolus vulgaris NAC domain protein NAC1. 281 G1463 gi14485513 0.055 Solanum tuberosum putative NAC domain protein. 281 G1463 gi2982275 0.063 Picea mariana ATAF1-like protein. 281 G1463 gi4218537 0.09 Triticum sp. GRAB2 protein. 283 G1471 BH512970 9.00E−14 Brassica oleracea BOHIV20TF BOHI Brassica oleracea genomic 283 G1471 BZ374146 0.015 Zea mays ie21f07.g2 WGS-ZmaysF (DH5a methyl filtered) Zea m 283 G1471 AI898615 0.02 Lycopersicon EST268058 tomato ovary, esculentum TAMU Lycope 283 G1471 BG646742 0.02 Medicago truncatula EST508361 HOGA Medicago truncatula cDNA 283 G1471 BI968516 0.026 Glycine max GM830005B12C03 Gm- r1083 Glycine max cDNA clone 283 G1471 AP004754 0.026 Oryza sativa (japonica ( ) chromosome 6 clo cultivar-group) 283 G1471 AAAA01022633 0.026 Oryza sativa (indica ( ) scaffold022633 cultivar-group) 283 G1471 AB006606 0.034 Petunia x hybrida mRNA for ZPT4-4, complete cds. 283 G1471 BU879483 0.058 Populus balsamifera V060G08 Populus flow subsp. trichocarpa 283 G1471 BM359777 0.058 Gossypium arboreum GA_Ea0023K21r Gossypium arboreum 7-10 d 283 G1471 gi439491 0.00043 Petunia x hybrida zinc-finger DNA binding protein. 283 G1471 gi1763063 0.0094 Glycine max SCOF-1. 283 G1471 gi15623820 0.012 Oryza sativa hypothetical protein. 283 G1471 gi18390109 0.049 Sorghum bicolor putative zinc finger protein. 283 G1471 gi2058504 0.074 Brassica rapa zinc-finger protein-1. 283 G1471 gi7228329 0.095 Medicago sativa putative TFIIIA (or kruppel)-like zinc fi 283 G1471 gi4666360 0.13 Datisca glomerata zinc-finger protein 1. 283 G1471 gi18674684 0.14 Zea ramosa unnamed protein product. 283 G1471 gi20804883 0.24 Oryza sativa (japonica putative zinc fing cultivar-group) 283 G1471 gi2981169 0.39 Nicotiana tabacum osmotic stress-induced zinc- finger prot 285 G1478 BH541785 8.00E−38 Brassica oleracea BOHPJ56TF BOHP Brassica oleracea genomic 285 G1478 BI122215 4.00E−23 Populus tremula x I003P84P Populus leaf Populus tremuloides 285 G1478 BF275913 5.00E−22 Gossypium arboreum GA_Eb0025C07f Gossypium arboreum 7-10 d 285 G1478 CA814858 1.00E−21 Vitis vinifera CA12EI201IIbF_F05 Cabernet Sauvignon Leaf- 285 G1478 BG157399 8.00E−20 Glycine max sab36g12.y1 Gm-c1026 Glycine max cDNA clone GEN 285 G1478 CA798224 3.00E−15 Theobroma cacao Cac_BL_5512 Cac_BL (Bean and Leaf from Amel 285 G1478 BU873581 6.00E−12 Populus balsamifera Q057B04 Populus flow subsp. trichocarpa 285 G1478 BU046688 2.00E−11 Prunus persica PP_LEa0027D08f Peach developing fruit mesoca 285 G1478 C95300 8.00E−11 Citrus unshiu C95300 Citrus unshiu Miyagawa-wase maturation 285 G1478 BQ594583 1.00E−10 Beta vulgaris E012444-024-024-P06-SP6 MPIZ-ADIS-024-develop 285 G1478 gi2895188 1.10E−11 Brassica napus CONSTANS homolog. 285 G1478 gi3618308 3.50E−10 Oryza sativa zinc finger protein. 285 G1478 gi23495871 1.10E−09 Oryza sativa (japonica putative zinc-fing cultivar-group) 285 G1478 gi11037308 1.10E−09 Brassica nigra constans-like protein. 285 G1478 gi3341723 3.10E−09 Raphanus sativus CONSTANS-like 1 protein. 285 G1478 gi4091806 3.60E−08 Malus x domestica CONSTANS-like protein 2. 285 G1478 gi21655168 3.70E−08 Hordeum vulgare subsp. CONSTANS-like protein vulgare CO8. 285 G1478 gi21667475 4.50E−08 Hordeum vulgare CONSTANS-like protein. 285 G1478 gi10946337 7.20E−08 Ipomoea nil CONSTANS-like protein. 285 G1478 gi4557093 3.30E−06 Pinus radiata zinc finger protein. 287 G1482 BM406201 9.00E−61 Solanum tuberosum EST580528 potato roots Solanum tuberosum 287 G1482 BF644868 1.00E−53 Medicago truncatula NF023D11EC1F1093 Elicited cell culture 287 G1482 BI678186 9.00E−53 Robinia pseudoacacia CLS1114 CLS (Cambium and bark region o 287 G1482 BM954087 4.00E−52 Glycine max sam70a09.y1 Gm-c1069 Glycine max cDNA clone SOY 287 G1482 BI420251 1.00E−48 Lotus japonicus LjNEST54g9r Lotus japonicus nodule library 287 G1482 AU288043 1.00E−45 Zinnia elegans AU288043 zinnia cultured mesophyll cell equa 287 G1482 BU892726 2.00E−45 Populus tremula P068F06 Populus petioles cDNA library Popul 287 G1482 BE432467 1.00E−44 Lycopersicon EST398996 tomato breaker esculentum fruit, TIG 287 G1482 AB001884 4.00E−43 Oryza sativa mRNA for zinc finger protein, complete cds, 287 G1482 BZ088073 6.00E−43 Brassica oleracea lla97a06.b1 B. oleracea002 Brassica olerac 287 G1482 gi3618312 1.60E−45 Oryza sativa zinc finger protein. 287 G1482 gi11037311 4.00E−18 Brassica nigra constans-like protein. 287 G1482 gi3341723 6.50E−17 Raphanus sativus CONSTANS-like 1 protein. 287 G1482 gi23589949 5.50E−16 Oryza sativa (japonica Hd1. cultivar-group) 287 G1482 gi4091806 6.00E−15 Malus x domestica CONSTANS-like protein 2. 287 G1482 gi10946337 1.60E−14 Ipomoea nil CONSTANS-like protein. 287 G1482 gi2303681 2.10E−14 Brassica napus unnamed protein product. 287 G1482 gi21667485 2.30E−13 Hordeum vulgare CONSTANS-like protein. 287 G1482 gi21655154 1.20E−11 Hordeum vulgare subsp. CONSTANS-like protein vulgare CO5. 287 G1482 gi4557093 2.50E−10 Pinus radiata zinc finger protein. 289 G1488 BH447680 5.00E−83 Brassica oleracea BOHQJ20TR BOHQ Brassica oleracea genomic 289 G1488 AP003376 7.00E−55 Oryza sativa chromosome 1 clone OSJNBa0014K08, *** SEQUENCI 289 G1488 AAAA01003594 3.00E−54 Oryza sativa (indica ( ) scaffold003594 cultivar-group) 289 G1488 AC132491 3.00E−54 Oryza sativa (japonica ( ) chromosome 5 clo cultivar-group) 289 G1488 BQ851743 3.00E−49 Lactuca sativa QGB16C22.yg.ab1 QG_ABCDI lettuce salinas Lac 289 G1488 BM113228 3.00E−49 Solanum tuberosum EST560764 potato roots Solanum tuberosum 289 G1488 BU547281 2.00E−45 Glycine max GM880012B20D06 Gm- r1088 Glycine max cDNA clone 289 G1488 BQ410000 3.00E−43 Gossypium arboreum GA_Ed0026H09r Gossypium arboreum 7-10 d 289 G1488 CA600585 6.00E−38 Triticum aestivum waw1c.pk005.k20 waw1c Triticum aestivum c 289 G1488 AC136451 2.00E−36 Medicago truncatula clone mth2-17d19, WORKING DRAFT SEQUENC 289 G1488 gi21902044 1.80E−48 Oryza sativa (japonica hypothetical prote cultivar-group) 289 G1488 gi14165317 4.10E−42 Oryza sativa putative transcription factor. 289 G1488 gi12711287 3.80E−30 Nicotiana tabacum GATA-1 zinc finger protein. 289 G1488 gi1076609 5.60E−22 Nicotiana NTL1 protein-curled- plumbaginifolia leaved to 289 G1488 gi14550106 0.85 Zea mays HD2 type histone deacetylase HDA106. 289 G1488 gi21953514 0.98 Zea mays subsp. ZAGL1. parviglumis 289 G1488 gi21953536 1 Zea mays subsp. mays ZAGL1. 291 G1494 BH695524 1.00E−66 Brassica oleracea BOMMP13TF BO_2_3_KB Brassica oleracea gen 291 G1494 BU866069 2.00E−47 Populus tremula x S062C11 Populus imbib Populus tremuloides 291 G1494 BG591063 1.00E−37 Solanum tuberosum EST498905 P. infestans- challenged leaf So 291 G1494 BF518953 2.00E−36 Medicago truncatula EST456346 DSIL Medicago truncatula cDNA 291 G1494 BM411362 1.00E−35 Lycopersicon EST585689 tomato breaker esculentum fruit Lyco 291 G1494 BE598711 5.00E−30 Sorghum bicolor PI1_81_D03.b1_A002 Pathogen induced 1 (PI1) 291 G1494 BU574318 6.00E−30 Prunus dulcis PA_Ea0007A10f Almond developing seed Prunus 291 G1494 CA008614 1.00E−29 Hordeum vulgare subsp. HU11I14r HU Hordeum vulgare vulgare 291 G1494 BG041496 3.00E−29 Glycine max sv35a08.y1 Gm-c1057 Glycine max cDNA clone GENO 291 G1494 BG052163 3.00E−27 Sorghum propinquum RHIZ2_6_H10.b1_A003 Rhizome2 (RHIZ2) Sor 291 G1494 gi23495742 1.90E−39 Oryza sativa (japonica putative phytochro cultivar-group) 291 G1494 gi13486760 4.50E−25 Oryza sativa hypothetical protein. 291 G1494 gi5923912 2.00E−10 Tulipa gesneriana bHLH transcription factor GBOF-1. 291 G1494 gi1086538 2.30E−09 Oryza rufipogon transcriptional activator Rb homolog. 291 G1494 gi527657 1.00E−08 Pennisetum glaucum myc-like regulatory R gene product. 291 G1494 gi527665 3.60E−08 Sorghum bicolor myc-like regulatory R gene product. 291 G1494 gi527661 7.60E−08 Phyllostachys acuta myc-like regulatory R gene product. 291 G1494 gi1086534 4.40E−07 Oryza officinalis transcriptional activator Ra homolog. 291 G1494 gi527663 4.40E−07 Tripsacum australe myc-like regulatory R gene product. 291 G1494 gi1142621 4.80E−07 Phaseolus vulgaris phaseolin G-box binding protein PG2. 293 G1496 BZ007786 2.00E−64 Brassica oleracea oed22d06.g1 B. oleracea002 Brassica olerac 293 G1496 BQ875608 3.00E−41 Lactuca sativa QGI8J14.yg.ab1 QG_ABCDI lettuce salinas Lact 293 G1496 BU081702 2.00E−40 Glycine max saq98c07.y1 Gm-c1049 Glycine max cDNA clone SOY 293 G1496 CA525194 3.00E−37 Capsicum annuum KS12050G08 KS12 Capsicum annuum cDNA, mRNA 293 G1496 BU791131 1.00E−36 Populus balsamifera subsp. trichocarpa x Populus deltoides 293 G1496 AW906522 2.00E−34 Solanum tuberosum EST342644 potato stolon, Cornell Universi 293 G1496 BF273293 2.00E−34 Gossypium arboreum GA_Eb0017H08f Gossypium arboreum 7-10 d 293 G1496 BJ267378 6.00E−34 Triticum aestivum BJ267378 Y. Ogihara unpublished cDNA libr 293 G1496 BM497415 4.00E−33 Avicennia marina 901269 Avicennia marina leaf cDNA Library 293 G1496 CA003238 1.00E−32 Hordeum vulgare subsp. HS09N06r HS Hordeum vulgare vulgare 293 G1496 gi20804997 5.10E−35 Oryza sativa (japonica DNA-binding protei cultivar-group) 293 G1496 gi11862964 9.50E−35 Oryza sativa hypothetical protein. 293 G1496 gi5923912 7.00E−31 Tulipa gesneriana bHLH transcription factor GBOF-1. 293 G1496 gi6166283 1.70E−10 Pinus taeda helix-loop-helix protein 1A. 293 G1496 gi527655 2.00E−05 Pennisetum glaucum myc-like regulatory R gene product. 293 G1496 gi527665 2.90E−05 Sorghum bicolor myc-like regulatory R gene product. 293 G1496 gi527661 5.50E−05 Phyllostachys acuta myc-like regulatory R gene product. 293 G1496 gi1086538 0.00019 Oryza rufipogon transcriptional activator Rb homolog. 293 G1496 gi4206118 0.00024 Mesembryanthemum transporter homolog. crystallinum 293 G1496 gi3399777 0.00025 Glycine max symbiotic ammonium transporter; nodulin. 295 G1499 AT002234 1.00E−53 Brassica rapa subsp. AT002234 Flower bud pekinensis cDNA Br 295 G1499 AP004462 1.00E−46 Oryza sativa (japonica ( ) chromosome 8 clo cultivar-group) 295 G1499 AAAA01003354 1.00E−46 Oryza sativa (indica ( ) scaffold003354 cultivar-group) 295 G1499 BH775806 1.00E−39 Zea mays fzmb011f018c05f1 fzmb filtered library Zea mays ge 295 G1499 BH700922 4.00E−35 Brassica oleracea BOMMZ07TR BO_2_3_KB Brassica oleracea gen 295 G1499 AP004693 1.00E−34 Oryza sativa chromosome 8 clone P0461F06, *** SEQUENCING IN 295 G1499 AW832545 5.00E−34 Glycine max sm12e10.y1 Gm-c1027 Glycine max cDNA clone GENO 295 G1499 BE451174 1.00E−32 Lycopersicon EST402062 tomato root, esculentum plants pre-a 295 G1499 BF263465 4.00E−25 Hordeum vulgare HV_CEa0006N02f Hordeum vulgare seedling gre 295 G1499 BG557011 5.00E−22 Sorghum bicolor EM1_41_E02.g1_A002 Embryo 1 (EM1) Sorghum b 295 G1499 gi15528743 2.50E−30 Oryza sativa contains EST C74560(E31855)~unknown protein. 295 G1499 gi19571105 2.80E−27 Oryza sativa (japonica hypothetical prote cultivar-group) 295 G1499 gi11045087 1.10E−08 Brassica napus putative protein. 295 G1499 gi3127045 6.20E−08 Petunia x hybrida bHLH transcription factor JAF13. 295 G1499 gi1086538 1.30E−07 Oryza rufipogon transcriptional activator Rb homolog. 295 G1499 gi6166283 1.60E−07 Pinus taeda helix-loop-helix protein 1A. 295 G1499 gi5923912 1.00E−06 Tulipa gesneriana bHLH transcription factor GBOF-1. 295 G1499 gi5669656 1.10E−06 Lycopersicon ER33 protein. esculentum 295 G1499 gi527665 1.40E−06 Sorghum bicolor myc-like regulatory R gene product. 295 G1499 gi1086534 3.10E−06 Oryza officinalis transcriptional activator Ra homolog. 297 G1519 AY107434 1.00E−131 Zea mays PCO110680 mRNA sequence. 297 G1519 BQ579759 4.00E−68 Triticum aestivum WHE2974_B12_D24ZS Wheat dormant embryo cD 297 G1519 BQ851827 3.00E−66 Lactuca sativa QGB16G12.yg.ab1 QG_ABCDI lettuce salinas Lac 297 G1519 BM094986 2.00E−61 Glycine max saj24f10.y1 Gm-c1066 Glycine max cDNA clone GEN 297 G1519 BE354396 3.00E−54 Lycopersicon EST355739 tomato flower esculentum buds, anthe 297 G1519 AW618704 7.00E−52 Lycopersicon pennellii EST320690 L. pennellii trichome, Cor 297 G1519 BF004323 4.00E−50 Medicago truncatula EST432821 KV1 Medicago truncatula cDNA 297 G1519 BU833376 2.00E−49 Populus tremula x T047C03 Populus apica Populus tremuloides 297 G1519 AJ470209 3.00E−45 Hordeum vulgare AJ470209 S00008 Hordeum vulgare cDNA clone 297 G1519 BF053939 2.00E−43 Solanum tuberosum EST439169 potato leaves and petioles Sola 297 G1519 gi14192879 1.00E−88 Oryza sativa Putative zinc-binding peroxisomal integral m 297 G1519 gi22535577 8.90E−08 Oryza sativa (japonica hypothetical prote cultivar-group) 297 G1519 gi22795037 4.30E−07 Populus x canescens putative RING protein. 297 G1519 gi9294812 0.0026 Medicago truncatula MTD2. 297 G1519 gi18129286 0.0028 Pinus pinaster putative RING zinc finger protein. 297 G1519 gi4090943 0.0029 Lycopersicon COP1 homolog. esculentum 297 G1519 gi22775495 0.0095 Arabis gemmifera similar to A. thaliana AT4g08590. 297 G1519 gi20340241 0.021 Thellungiella halophila putative RING zinc finger protein 297 G1519 gi1694900 0.022 Pisum sativum Cop1 protein. 297 G1519 gi7592844 0.029 Oryza sativa subsp. COP1. japonica 299 G1526 AAAA01000691 1.00E−103 Oryza sativa (indica ( ) scaffold000691 cultivar-group) 299 G1526 BG599126 1.00E−101 Solanum tuberosum EST504026 cSTS Solanum tuberosum cDNA clo 299 G1526 BI098460 7.00E−94 Sorghum bicolor IP1_32_F12.b1_A002 Immature pannicle 1 (IP1 299 G1526 AY110582 4.00E−74 Zea mays CL19105_1 mRNA sequence. 299 G1526 AJ468417 8.00E−69 Hordeum vulgare AJ468417 S00008 Hordeum vulgare cDNA clone 299 G1526 AL819754 9.00E−68 Triticum aestivum AL819754 n: 129 Triticum aestivum cDNA clo 299 G1526 AW011575 2.00E−66 Pinus taeda ST22D10 Pine TriplEx shoot tip library Pinus ta 299 G1526 AW704900 6.00E−63 Glycine max sk40h12.y1 GM-c1019 Glycine max cDNA clone GENO 299 G1526 AP004879 4.00E−60 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 299 G1526 BQ589890 1.00E−57 Beta vulgaris S015141-024-019-P15-SP6 MPIZ-ADIS-024-storage 299 G1526 gi23237908 4.20E−115 Oryza sativa (japonica helicase-like tran cultivar-group) 299 G1526 gi15289872 5.60E−80 Oryza sativa putative helicase-like transcription factor. 299 G1526 gi18463957 1.90E−42 Zea mays chromatin complex subunit A101. 299 G1526 gi23193481 8.70E−32 Hordeum vulgare SNF2P. 299 G1526 gi23193487 9.70E−32 Triticum monococcum SNF2P. 299 G1526 gi23193479 2.50E−30 Hordeum vulgare subsp. SNF2P. vulgare 299 G1526 gi15029364 0.0012 Rosa hybrid cultivar photoregulatory zinc-finger protein 299 G1526 gi1694900 0.0052 Pisum sativum Cop1 protein. 299 G1526 gi7592844 0.011 Oryza sativa subsp. COP1. japonica 299 G1526 gi4090943 0.014 Lycopersicon COP1 homolog. esculentum 301 G1540 BZ081838 4.00E−70 Brassica oleracea 11f51h03.g1 B. oleracea002 Brassica olerac 301 G1540 AF481951 7.00E−41 Petunia × hybrida wuschel protein (WUS) mRNA, complete eds. 301 G1540 AAAA01000169 2.00E−28 Oryza sativa (indica ( ) scaffold000169 cultivar-group) 301 G1540 OSJN00127 2.00E−28 Oryza sativa chromosome 4 clone OSJNBA0084K01, *** SEQUENC 301 G1540 AX105289 2.00E−26 Zea mays Sequence 7 from Patent WO0123575. 301 G1540 AC137078 2.00E−21 Medicago truncatula clone mth2-10e12, WORKING DRAFT SEQUENC 301 G1540 BI204369 5.00E−20 Lycopersicon EST522409 cTOS esculentum Lycopersicon esculen 301 G1540 CNS08CDT 1.00E−19 Oryza sativa (japonica ( ) chromosome 12 cl cultivar-group) 301 G1540 BU006325 2.00E−19 Lactuca sativa QGH10L09.yg.ab1 QG_EFGHJ lettuce serriola La 301 G1540 AF322401 3.00E−19 Vigna radiata clone LR129 microsatellite sequence. 301 G1540 gi22087128 5.10E−35 Petunia x hybrida wuschel protein. 301 G1540 gi8099120 3.90E−21 Oryza sativa similar to a putative homeodomain transcript 301 G1540 gi21104626 6.40E−21 Oryza sativa (japonica hypothetical prote cultivar-group) 301 G1540 gi3955021 1.80E−09 Populus tremula x HB2 homeodomain pro Populus tremuloides 301 G1540 gi18419580 0.00033 Narcissus putative homeobox- pseudonarcissus containing pr 301 G1540 gi3868829 0.0014 Ceratopteris richardii CRHB1. 301 G1540 gi24417147 0.0024 Zinnia elegans homeobox leucine-zipper protein. 301 G1540 gi7209912 0.14 Physcomitrella patens homeobox protein PpHB10. 301 G1540 gi13365610 0.74 Pisum sativum SCARECROW. 301 G1540 gi1160484 0.94 Pimpinella brachycarpa homeobox-leucine zipper protein. 303 G1543 AF145727 4.00E−51 Oryza sativa homeodomain leucine zipper protein (hox3) mRNA 303 G1543 CA030381 6.00E−41 Hordeum vulgare subsp. HX06O07r HX Hordeum vulgare vulgare 303 G1543 BQ741095 6.00E−39 Glycine max saq14c10.y1 Gm-c1045 Glycine max cDNA clone SOY 303 G1543 AT002118 1.00E−38 Brassica rapa subsp. AT002118 Flower bud pekinensis cDNA Br 303 G1543 BQ857226 2.00E−37 Lactuca sativa QGB6P03.yg.ab1 QG_ABCDI lettuce salinas Lact 303 G1543 AB028075 4.00E−37 Physcomitrella patens mRNA for homeobox protein PpHB4, comp 303 G1543 PBPHZ4GEN 4.00E−37 Pimpinella brachycarpa P. brachycarpa mRNA for homeobox-leu 303 G1543 LEHDZIPP 5.00E−37 Lycopersicon L. esculentum mRNA for esculentum HD-ZIP protei 303 G1543 AF443619 1.00E−36 Craterostigma homeodomain leucine plantagineum zipper prote 303 G1543 AJ498394 2.00E−36 Medicago truncatula AJ498394 MTPOSE Medicago truncatula cDN 303 G1543 gi5006851 8.30E−51 Oryza sativa homeodomain leucine zipper protein. 303 G1543 gi20161555 1.70E−50 Oryza sativa (japonica putative homeodoma cultivar-group) 303 G1543 gi18034437 1.60E−38 Craterostigma homeodomain leucine plantagineum zipper pro 303 G1543 gi1149535 4.30E−38 Pimpinella brachycarpa homeobox-leucine zipper protein. 303 G1543 gi992598 1.20E−37 Lycopersicon HP-ZIP protein. esculentum 303 G1543 gi7415620 1.50E−37 Physcomitrella patens homeobox protein PpHB4. 303 G1543 gi1234900 3.10E−37 Glycine max homeobox-leucine zipper protein. 303 G1543 gi3868847 1.90E−35 Ceratopteris richardii CRHB10. 303 G1543 gi8919876 1.90E−35 Capsella rubella hypothetical protein. 303 G1543 gi1032372 3.20E−35 Helianthus annuus homeodomain protein. 305 G1634 AW164275 6.00E−63 Glycine max se70d01.y1 Gm-c1023 Glycine max cDNA clone GENO 305 G1634 AF239956 3.00E−60 Hevea brasiliensis unknown mRNA. 305 G1634 BQ115848 9.00E−58 Solanum tuberosum EST601424 mixed potato tissues Solanum tu 305 G1634 AW220831 5.00E−53 Lycopersicon EST297300 tomato fruit esculentum mature green 305 G1634 BQ992139 9.00E−53 Lactuca sativa QGF24M24.yg.ab1 QG_EFGHJ lettuce serriola La 305 G1634 BG525326 3.00E−46 Stevia rebaudiana 48-70 Stevia field grown leaf cDNA Stevia 305 G1634 BE319813 2.00E−45 Medicago truncatula NF022C09RT1F1066 Developing root Medica 305 G1634 AP003279 3.00E−45 Oryza sativa chromosome 1 clone P0529E05, *** SEQUENCING IN 305 G1634 AAAA01017329 3.00E−45 Oryza sativa (indica ( ) scaffold017329 cultivar-group) 305 G1634 AC130612 3.00E−45 Oryza sativa (japonica ( ) chromosome 5 clo cultivar-group) 305 G1634 gi12005328 7.40E−59 Hevea brasiliensis unknown. 305 G1634 gi18874263 1.10E−55 Antirrhinum majus MYB-like transcription factor DIVARICAT 305 G1634 gi18461206 2.80E−50 Oryza sativa (japonica contains ESTs AU10 cultivar-group) 305 G1634 gi10798825 2.10E−45 Oryza sativa putative myb-related transcription activator 305 G1634 gi19911579 6.60E−42 Glycine max syringolide-induced protein 1-3-1B. 305 G1634 gi15209176 2.00E−41 Solanum demissum putative I-box binding factor. 305 G1634 gi6688529 2.30E−39 Lycopersicon I-box binding factor. esculentum 305 G1634 gi12406995 3.30E−24 Hordeum vulgare MCB2 protein. 305 G1634 gi7705206 2.30E−23 Solanum tuberosum MybSt1. 305 G1634 gi20067661 3.40E−18 Zea mays one repeat myb transcriptional factor. 307 G1637 BZ0113S1 4.00E−81 Brassica oleracea oed23f03.b1 B. oleracea002 Brassica olerac 307 G1637 BE033910 3.00E−42 Mesembryanthemum MG01H12 MG crystallinum Mesembryanthemum c 307 G1637 AY151044 9.00E−39 Oryza sativa (japonica ( ) transcription fa cultivar-group) 307 G1637 BU832707 2.00E−38 Populus tremula x T037C12 Populus apica Populus tremuloides 307 G1637 CA728673 3.00E−38 Triticum aestivum wdi1c.pk004.124 wdi1c Triticum aestivum c 307 G1637 BG454685 1.00E−37 Medicago truncatula NF102F10LF1F1080 Developing leaf Medica 307 G1637 CA799375 1.00E−37 Glycine max sat32h04.y1 Gm-c1056 Glycine max cDNA clone SOY 307 G1637 BJ472691 2.00E−37 Hordeum vulgare subsp. BJ472691 K. Sato vulgare unpublished 307 G1637 CA813590 3.00E−37 Vitis vinifera CA48LU10IVF-G11 CA48LU Vitis vinifera cDNA c 307 G1637 BQ114109 8.00E−37 Solanum tuberosum EST599685 mixed potato tissues Solanum tu 307 G1637 gi13569996 1.10E−39 Oryza sativa putative Myb-related protein. 307 G1637 gi24850307 2.30E−39 Oryza sativa (japonica transcription fact cultivar-group) 307 G1637 gi1076660 1.70E−36 Solanum tuberosum D13F(MYBST1) protein- potato. 307 G1637 gi12406993 8.90E−31 Hordeum vulgare MCB1 protein. 307 G1637 gi12005328 3.60E−27 Hevea brasiliensis unknown. 307 G1637 gi18874263 2.50E−26 Antirrhinum majus MYB-like transcription factor DIVARICAT 307 G1637 gi19911577 1.40E−25 Glycine max syringolide-induced protein 1-3-1A. 307 G1637 gi6688529 3.00E−23 Lycopersicon I-box binding factor. esculentum 307 G1637 gi15209176 7.60E−21 Solanum demissum putative I-box binding factor. 307 G1637 gi20067661 3.40E−16 Zea mays one repeat myb transcriptional factor. 309 G1640 AF034132 3.00E−60 Gossypium hirsutum MYB-like DNA-binding domain protein (Cmy 309 G1640 AV421866 6.00E−57 Lotus japonicus AV421866 Lotus japonicus young plants (two- 309 G1640 ZMU57002 8.00E−57 Zea mays P protein (P) mRNA, complete cds. 309 G1640 BI924574 2.00E−56 Lycopersicon EST544463 tomato flower, esculentum buds 0-3 m 309 G1640 AW255172 2.00E−55 Mentha x piperita ML160 peppermint glandular trichome Menth 309 G1640 BE558747 3.00E−54 Hordeum vulgare HV_CEb0020E02f Hordeum vulgare seedling gre 309 G1640 AW186273 1.00E−51 Glycine max se65f12.y1 Gm-c1019 Glycine max cDNA clone GENO 309 G1640 PMU39448 2.00E−50 Picea mariana MYB-like transcriptional factor MBF1 mRNA, co 309 G1640 BQ865372 3.00E−50 Lactuca sativa QGC4a02.yg.ab1 QG_ABCDI lettuce salinas Lact 309 G1640 BQ046535 8.00E−49 Solanum tuberosum EST595653 P. infestans- challenged potato 309 G1640 gi12060532 7.40E−59 Oryza sativa putative myb-related protein P. 309 G1640 gi2921336 2.00E−58 Gossypium hirsutum MYB-like DNA-binding domain protein. 309 G1640 gi11526779 2.00E−56 Zea mays subsp. P-like protein. parviglumis 309 G1640 gi11526773 5.40E−56 Zea mays P2 protein. 309 G1640 gi1101770 4.60E−50 Picea mariana MYB-like transcriptional factor MBF1. 309 G1640 gi82308 8.60E−49 Antirrhinum majus myb protein 308-garden snapdragon. 309 G1640 gi1370140 8.70E−49 Lycopersicon myb-related transcription esculentum factor. 309 G1640 gi5139802 1.80E−48 Glycine max GmMYB29A1. 309 G1640 gi127579 1.30E−47 Hordeum vulgare MYB-RELATED PROTEIN HV1. 309 G1640 gi227030 1.30E−47 Hordeum vulgare var. myb-related gene Hv1. distichum 311 G1645 AW624217 9.00E−59 Lycopersicon EST322258 tomato flower esculentum buds 3-8 mm 311 G1645 AQ917084 1.00E−54 Medicago truncatula T233110b Medicago truncatula BAC librar 311 G1645 AP005757 4.00E−53 Oryza sativa (japonica ( ) chromosome 8 clo cultivar-group) 311 G1645 AAAA01001041 4.00E−53 Oryza sativa (indica ( ) scaffold001041 cultivar-group) 311 G1645 BQ514458 7.00E−53 Solanum tuberosum EST621873 Generation of a set of potato c 311 G1645 BF270511 3.00E−51 Gossypium arboreum GA_Eb0008O08f Gossypium arboreum 7-10 d 311 G1645 AP000837 7.00E−51 Oryza sativa genomic DNA, chromosome 1, clone: P0424A08. 311 G1645 AX288143 5.00E−49 Physcomitrella patens Sequence 14 from Patent WO0177311. 311 G1645 AI164087 1.00E−48 Populus tremula x A054P76U Hybrid aspen Populus tremuloides 311 G1645 BQ623005 1.00E−46 Citrus sinensis USDA-FP_00096 Ridge pineapple sweet orange 311 G1645 gi6539552 4.80E−58 Oryza sativa Similar to putative transcription factor (AF 311 G1645 gi21321780 1.40E−46 Oryza sativa (japonica putative Myb/Myb-r cultivar-group) 311 G1645 gi9954112 2.10E−34 Solanum tuberosum tuber-specific and sucrose- responsive e 311 G1645 gi20565 2.30E−29 Petunia x hybrida protein 3. 311 G1645 gi7230673 6.40E−29 Papaver rhoeas putative Myb-related domain. 311 G1645 gi16326133 2.80E−28 Nicotiana tabacum Myb. 311 G1645 gi8745321 2.90E−28 Physcomitrella patens putative c-myb-like transcription f 311 G1645 gi8745325 3.10E−28 Hordeum vulgare putative c-myb-like transcription factor. 311 G1645 gi7677132 5.10E−28 Secale cereale c-myb-like transcription factor. 311 G1645 gi7677136 1.30E−27 Adiantum raddianum c-myb-like transcription factor. 313 G1646 AW776719 3.00E−88 Medicago truncatula EST335784 DSIL Medicago truncatula cDNA 313 G1646 BG591677 6.00E−87 Solanum tuberosum EST499519 P. infestans- challenged leaf So 313 G1646 BQ411597 4.00E−85 Gossypium arboreum GA_Ed001B06f Gossypium arboreum 7-10 d 313 G1646 BE208917 2.00E−84 Citrus x paradisi GF-FV-P3F5 Marsh grapefruit young flavedo 313 G1646 BM065544 1.00E−83 Capsicum annuum KS07004F12 KS07 Capsicum annuum cDNA, mRNA 313 G1646 BQ860015 1.00E−79 Lactuca sativa QGC14J23.yg.ab1 QG_ABCDI lettuce salinas Lac 313 G1646 BI701620 3.00E−79 Glycine max sai18a04.y1 Gm-c1053 Glycine max cDNA clone GEN 313 G1646 BH725354 2.00E−77 Brassica oleracea BOHVO37TF BO_2_3_KB Brassica oleracea gen 313 G1646 AW093662 2.00E−73 Lycopersicon EST286842 tomato mixed esculentum elicitor, BT 313 G1646 BI127986 7.00E−67 Populus tremula x G069P33Y Populus camb Populus tremuloides 313 G1646 gi5257260 6.10E−48 Oryza sativa Similar to sequence of BAC F7G19 from Arabid 313 G1646 gi20804442 2.30E−21 Oryza sativa (japonica hypothetical prote cultivar-group) 313 G1646 gi18481626 5.00E−08 Zea mays repressor protein. 313 G1646 gi169345 0.028 Phaseolus vulgaris hydroxyproline-rich glycoprotein. 313 G1646 gi19700533 0.039 Pyrus communis unnamed protein product. 313 G1646 gi2108256 0.095 Bromheadia extensin. finlaysoniana 313 G1646 gi1778097 0.1 Pinus taeda expansin. 313 G1646 gi347455 0.12 Glycine max hydroxyproline-rich glycoprotein. 313 G1646 gi4105119 0.26 Hordeum vulgare dehydrin 10. 313 G1646 gi1076601 0.39 Lycopersicon structural cell wall protein- esculentum to 315 G1652 AI896266 6.00E−45 Lycopersicon EST265709 tomato callus, esculentum TAMU Lycop 315 G1652 AI967554 2.00E−44 Lotus japonicus Ljirnpest05-403-e2 Ljirnp Lambda HybriZap t 315 G1652 BU884552 2.00E−43 Populus tremula x R012C01 Populus root Populus tremuloides 315 G1652 AF069738 1.00E−42 Glycine max symbiotic ammonium transporter (SAT1) mRNA, com 315 G1652 AW775712 2.00E−40 Medicago truncatula EST334777 DSIL Medicago truncatula cDNA 315 G1652 AF097665 3.00E−40 Mesembryanthemum transporter homolog mRNA, crystallinum par 315 G1652 AAAA01000416 4.00E−36 Oryza sativa (indica ( ) scaffold000416 cultivar-group) 315 G1652 BQ483543 7.00E−31 Triticum aestivum WHE3509_H02_O03ZS Wheat unstressed root c 315 G1652 AC099732 1.00E−26 Oryza sativa (japonica ( ) chromosome 3 clo cultivar-group) 315 G1652 BF253652 2.00E−24 Hordeum vulgare HVSMEf0001L22f Hordeum vulgare seedling roo 315 G1652 gi3399777 6.40E−44 Glycine max symbiotic ammonium transporter; nodulin. 315 G1652 gi4206118 8.50E−42 Mesembryanthemum transporter homolog. crystallinum 315 G1652 gi20532320 1.10E−32 Oryza sativa (japonica Putative bHLH tran cultivar-group) 315 G1652 gi18542931 2.40E−28 Oryza sativa Putative bHLH transcription factor. 315 G1652 gi1142619 2.70E−23 Phaseolus vulgaris phaseolin G-box binding protein PG1. 315 G1652 gi4321762 4.30E−17 Zea mays transcription factor MYC7E. 315 G1652 gi6175252 1.10E−14 Lycopersicon jasmonic acid 3. esculentum 315 G1652 gi10998404 1.80E−13 Petunia x hybrida anthocyanin 1. 315 G1652 gi527657 1.80E−12 Pennisetum glaucum myc-like regulatory R gene product. 315 G1652 gi527661 5.10E−12 Phyllostachys acuta myc-like regulatory R gene product. 317 G1672 BQ148509 9.00E−86 Medicago truncatula NF069A08FL1F1065 Developing flower Medi 317 G1672 BH478545 4.00E−82 Brassica oleracea BOHSE63TR BOHS Brassica oleracea genomic 317 G1672 BI129724 3.00E−72 Populus tremula x G094P85Y Populus camb Populus tremuloides 317 G1672 BI960052 5.00E−71 Hordeum vulgare HVSMEn0023A06f Hordeum vulgare rachis EST 1 317 G1672 AC124143 5.00E−69 Oryza sativa (japonica ( ) chromosome 5 clo cultivar-group) 317 G1672 AAAA01011028 7.00E−69 Oryza sativa (indica ( ) scaffold011028 cultivar-group) 317 G1672 BM527360 3.00E−66 Glycine max sal60h11.y1 Gm-c1061 Glycine max cDNA clone SOY 317 G1672 BF518231 2.00E−65 Pinus taeda NXSI_036_F03_F NXSI (Nsf Xylem Side wood Inclin 317 G1672 BQ508125 8.00E−61 Solanum tuberosum EST615540 Generation of a set of potato c 317 G1672 BE403509 1.00E−58 Triticum aestivum WHE0427_D02_H03ZS Wheat etiolated seedlin 317 G1672 gi9049470 7.10E−78 Oryza sativa hypothetical protein. 317 G1672 gi18461166 6.30E−69 Oryza sativa (japonica contains ESTs AU09 cultivar-group) 317 G1672 gi12751304 1.60E−47 Brassica napus CUC2-like protein. 317 G1672 gi7716952 5.40E−07 Medicago truncatula NAC1. 317 G1672 gi6732156 9.90E−07 Triticum monococcum unnamed protein product. 317 G1672 gi21389170 1.50E−06 Petunia x hybrida nam-like protein 16. 317 G1672 gi6175246 3.10E−06 Lycopersicon jasmonic acid 2. esculentum 317 G1672 gi4218537 0.00019 Triticum sp. GRAB2 protein. 317 G1672 gi15148912 0.00051 Phaseolus vulgaris NAC domain protein NAC1. 317 G1672 gi22597158 0.00071 Glycine max no apical meristem-like protein. 319 G1677 BU926268 7.00E−68 Glycine max sas88f08.y1 Gm-c1036 Glycine max cDNA clone SOY 319 G1677 BH519017 2.00E−59 Brassica oleracea BOHHW49TR BOHH Brassica oleracea genomic 319 G1677 BF649854 4.00E−58 Medicago truncatula NF085A08EC1F1055 Elicited cell culture 319 G1677 BI422020 3.00E−57 Lycopersicon EST532686 tomato callus, esculentum TAMU Lycop 319 G1677 BU894596 3.00E−48 Populus tremula x X011H04 Populus wood Populus tremuloides 319 G1677 BF625246 1.00E−47 Hordeum vulgare HVSMEa0008A15f Hordeum vulgare seedling sho 319 G1677 CA810372 1.00E−46 Vitis vinifera CA22LI05IF-C9 CA22LI Vitis vinifera cDNA clo 319 G1677 BQ118483 2.00E−46 Solanum tuberosum EST604059 mixed potato tissues Solanum tu 319 G1677 AB028183 2.00E−46 Oryza sativa mRNA for OsNAC4 protein, complete cds. 319 G1677 AF402603 6.00E−46 Phaseolus vulgaris NAC domain protein NAC2 mRNA, complete c 319 G1677 gi20303588 3.40E−54 Oryza sativa (japonica putative NAM (no a cultivar-group) 319 G1677 gi10697197 8.70E−49 Oryza sativa putative NAM protein. 319 G1677 gi21105748 5.30E−47 Petunia x hybrida nam-like protein 10. 319 G1677 gi4218535 1.80E−44 Triticum sp. GRAB1 protein. 319 G1677 gi6732158 1.80E−44 Triticum monococcum unnamed protein product. 319 G1677 gi14485513 1.60E−43 Solanum tuberosum putative NAC domain protein. 319 G1677 gi15148914 4.10E−42 Phaseolus vulgaris NAC domain protein NAC2. 319 G1677 gi6175246 9.70E−41 Lycopersicon jasmonic acid 2. esculentum 319 G1677 gi22597158 1.00E−38 Glycine max no apical meristem-like protein. 319 G1677 gi7716952 7.30E−38 Medicago truncatula NAC1. 321 G1749 BH723520 3.00E−44 Brassica oleracea BOHTN77TF BO_2_3_KB Brassica oleracea gen 321 G1749 AW559374 3.00E−22 Medicago truncatula EST314422 DSIR Medicago truncatula cDNA 321 G1749 AW152963 1.00E−17 Glycine max se33c03.y1 Gm-c1015 Glycine max cDNA clone GENO 321 G1749 BI422101 2.00E−16 Lycopersicon EST532767 tomato callus, esculentum TAMU Lycop 321 G1749 AP005418 2.00E−15 Oryza sativa (japonica ( ) chromosome 9 clo cultivar-group) 321 G1749 AAAA01002932 2.00E−15 Oryza sativa (indica ( ) scaffold002932 cultivar-group) 321 G1749 BU998389 2.00E−14 Hordeum vulgare subsp. HI10O11r HI Hordeum vulgare vulgare 321 G1749 BQ469024 4.00E−14 Hordeum vulgare HM03C08r HM Hordeum vulgare cDNA clone HM03 321 G1749 CA728820 4.00E−13 Triticum aestivum wdi1c.pk005.j15 wdi1c Triticum aestivum c 321 G1749 BQ803638 2.00E−12 Triticum monococcum WHE2839_H12_P23ZS Triticum monococcum v 321 G1749 gi20160854 1.30E−15 Oryza sativa (japonica hypothetical prote cultivar-group) 321 G1749 gi21740822 4.90E−14 Oryza sativa OSJNBa0042L16.10. 321 G1749 gi8809573 2.10E−13 Nicotiana sylvestris ethylene-responsive element binding 321 G1749 gi1208496 5.60E−13 Nicotiana tabacum EREBP-3. 321 G1749 gi20340233 5.60E−13 Thellungiella halophila ethylene responsive element bindi 321 G1749 gi3264767 1.20E−12 Prunus armeniaca AP2 domain containing protein. 321 G1749 gi18266198 1.90E−12 Narcissus AP-2 domain containing pseudonarcissus protein. 321 G1749 gi4099914 4.00E−12 Stylosanthes hamata ethylene-responsive element binding p 321 G1749 gi24940524 4.70E−12 Triticum aestivum ethylene response element binding prote 321 G1749 gi18535580 5.10E−12 Lycopersicon putative transcriptional esculentum activato 323 G1750 BH459103 8.00E−61 Brassica oleracea BOGEX73TR BOGE Brassica oleracea genomic 323 G1750 AP004902 7.00E−44 Lotus japonicus genomic DNA, chromosome 2, clone: LjT04G24, 323 G1750 AW685524 9.00E−39 Medicago truncatula NF031C12NR1F1000 Nodulated root Medicag 323 G1750 LEU89257 4.00E−36 Lycopersicon DNA-binding protein Pti6 esculentum mRNA, comp 323 G1750 BM886518 1.00E−35 Glycine max sam17f08.y1 Gm-c1068 Glycine max cDNA clone SOY 323 G1750 AF058827 5.00E−32 Nicotiana tabacum TSI1 (Tsi1) mRNA, complete cds. 323 G1750 BQ873772 4.00E−30 Lactuca sativa QGI2I03.yg.ab1 QG_ABCDI lettuce salinas Lact 323 G1750 AP002835 1.00E−28 Oryza sativa genomic DNA, chromosome 1, PAC clone: P0417G05. 323 G1750 AAAA01000263 3.00E−28 Oryza sativa (indica ( ) scaffold000263 cultivar-group) 323 G1750 BQ507568 1.00E−23 Solanum tuberosum EST614971 Generation of a set of potato c 323 G1750 gi2213785 5.10E−35 Lycopersicon Pti6. esculentum 323 G1750 gi8096469 1.50E−33 Oryza sativa Similar to Arabidopsis thaliana chromosome 4 323 G1750 gi3065895 1.10E−32 Nicotiana tabacum TSI1. 323 G1750 gi7528276 1.90E−21 Mesembryanthemum AP2-related transcription f crystallinum 323 G1750 gi8571476 2.50E−21 Atriplex hortensis apetala2 domain-containing protein. 323 G1750 gi8809575 1.30E−20 Nicotiana sylvestris ethylene-responsive element binding 323 G1750 gi17385636 1.70E−20 Matricaria chamomilla ethylene-responsive element binding 323 G1750 gi24060156 9.30E−20 Oryza sativa (japonica contains ESTs AU16 cultivar-group) 323 G1750 gi4099914 1.50E−19 Stylosanthes hamata ethylene-responsive element binding p 323 G1750 gi3264767 1.90E−19 Prunus armeniaca AP2 domain containing protein. 325 G1756 BH509555 1.00E−66 Brassica oleracea BOHIT47TF BOHI Brassica oleracea genomic 325 G1756 BU837263 4.00E−42 Populus tremula x T096G05 Populus apica Populus tremuloides 325 G1756 AW596933 2.00E−38 Glycine max sj84f07.y1 Gm-c1034 Glycine max cDNA clone GENO 325 G1756 AV423663 7.00E−38 Lotus japonicus AV423663 Lotus japonicus young plants (two- 325 G1756 BI923414 6.00E−37 Lycopersicon EST543319 tomato callus esculentum Lycopersico 325 G1756 BM112869 6.00E−32 Solanum tuberosum EST560405 potato roots Solanum tuberosum 325 G1756 BF519892 7.00E−32 Medicago truncatula EST457357 DSIL Medicago truncatula cDNA 325 G1756 AAAA01007990 5.00E−30 Oryza sativa (indica ( ) scaffold007990 cultivar-group) 325 G1756 AP004683 5.00E−30 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 325 G1756 AW447931 3.00E−29 Triticum aestivum BRY_1082 BRY Triticum aestivum cDNA clone 325 G1756 gi11761072 3.00E−30 Oryza sativa hypothetical protein. 325 G1756 gi4322940 1.20E−23 Nicotiana tabacum DNA-binding protein 2. 325 G1756 gi4894963 2.20E−20 Avena sativa DNA-binding protein WRKY3. 325 G1756 gi1432056 5.70E−20 Petroselinum crispum WRKY3. 325 G1756 gi11993901 1.60E−19 Dactylis glomerata somatic embryogenesis related protein. 325 G1756 gi13620227 2.00E−18 Lycopersicon hypothetical protein. esculentum 325 G1756 gi23305051 2.50E−18 Oryza sativa (indica WRKY transcription f cultivar-group) 325 G1756 gi18158619 2.30E−17 Retama raetam WRKY-like drought- induced protein. 325 G1756 gi22830985 7.60E−17 Oryza sativa (japonica WRKY transcription cultivar-group) 325 G1756 gi7484759 1.70E−16 Cucumis sativus SP8 binding protein homolog-cucumber. 327 G1765 BF649854 7.00E−74 Medicago truncatula NF085A08EC1F1055 Elicited cell culture 327 G1765 BI421877 2.00E−70 Lycopersicon EST532543 tomato callus, esculentum TAMU Lycop 327 G1765 BG511369 8.00E−60 Glycine max sad17a06.y1 Gm-c1074 Glycine max cDNA clone GEN 327 G1765 CA810372 9.00E−53 Vitis vinifera CA22LI05IF-C9 CA22LI Vitis vinifera cDNA clo 327 G1765 BH519017 3.00E−47 Brassica oleracea BOHHW49TR BOHH Brassica oleracea genomic 327 G1765 BQ586991 2.00E−46 Beta vulgaris E012352-024-011-F06-SP6 MPIZ-ADIS-024-leaf Be 327 G1765 BQ516602 9.00E−45 Solanum tuberosum EST624017 Generation of a set of potato c 327 G1765 BE034140 4.00E−44 Mesembryanthemum MG05E02 MG crystallinum Mesembryanthemum c 327 G1765 AF509873 8.00E−44 Petunia x hybrida nam-like protein 10 (NH10) mRNA, complete 327 G1765 BU883830 2.00E−43 Populus tremula x R002A08 Populus root Populus tremuloides 327 G1765 gi20303588 1.60E−67 Oryza sativa (japonica putative NAM (no a cultivar-group) 327 G1765 gi6175246 3.30E−47 Lycopersicon jasmonic acid 2. esculentum 327 G1765 gi21105748 5.60E−45 Petunia x hybrida nam-like protein 10. 327 G1765 gi15148914 1.70E−43 Phaseolus vulgaris NAC domain protein NAC2. 327 G1765 gi15528779 9.40E−43 Oryza sativa development regulation gene OsNAC4. 327 G1765 gi22597158 1.40E−41 Glycine max no apical meristem-like protein. 327 G1765 gi14485513 3.70E−41 Solanum tuberosum putative NAC domain protein. 327 G1765 gi4218537 1.00E−38 Triticum sp. GRAB2 protein. 327 G1765 gi6732160 1.00E−38 Triticum monococcum unnamed protein product. 327 G1765 gi7716952 7.30E−36 Medicago truncatula NAC1. 329 G1777 BQ996439 1.00E−120 Lactuca sativa QGG12N12.yg.ab1 QG_EFGHJ lettuce serriola La 329 G1777 BM985639 1.00E−101 Thellungiella halophila 2_F04_T3 Ath Thellungiella halophil 329 G1777 BM887188 4.00E−93 Glycine max sam35d01.y1 Gm-c1068 Glycine max cDNA clone SOY 329 G1777 BM661323 5.00E−87 Zea mays 952046G05.y1 952-BMS tissue from Walbot Lab (red 329 G1777 BU026535 9.00E−86 Helianthus annuus QHG17C11.yg.ab1 QH_EFGHJ sunflower RHA280 329 G1777 BH998711 1.00E−84 Brassica oleracea oep82h07.g1 B. oleracea002 Brassica olerac 329 G1777 AAAA01003274 1.00E−76 Oryza sativa (indica ( ) scaffold003274 cultivar-group) 329 G1777 AC103891 2.00E−76 Oryza sativa chromosome 3 clone OJ1175C11, *** SEQUENCING I 329 G1777 BG136684 1.00E−75 Lycopersicon pennellii EST477126 wild tomato pollen Lycoper 329 G1777 BG600834 4.00E−72 Solanum tuberosum EST505729 cSTS Solanum tuberosum cDNA clo 329 G1777 gi20330766 1.10E−199 Oryza sativa (japonica Putative RING zinc cultivar-group) 329 G1777 gi1666171 4.90E−35 Nicotiana unknown. plumbaginifolia 329 G1777 gi1362039 0.76 Fragaria x ananassa hypothetical protein (clone RJ39)-g 329 G1777 gi2244705 1 Nicotiana excelsior gamma-thionin. 331 G1792 AI776626 5.00E−35 Lycopersicon EST257726 tomato esculentum resistant, Cornell 331 G1792 BQ045702 1.00E−32 Solanum tuberosum EST594820 P. infestans- challenged potato 331 G1792 BM178875 7.00E−32 Glycine max saj60f01.y1 Gm-c1072 Glycine max cDNA clone SOY 331 G1792 BF649790 1.00E−31 Medicago truncatula NF084C07EC1F1052 Elicited cell culture 331 G1792 BZ020356 1.00E−30 Brassica oleracea oeg04a10.g1 B. oleracea002 Brassica olerac 331 G1792 BZ337899 3.00E−30 Sorghum bicolor ia91f11.b1 WGS-SbicolorF (JM107 adapted met 331 G1792 AC025907 3.00E−30 Oryza sativa chromosome 10 clone nbxb0094K20, *** SEQUENCIN 331 G1792 AAAA01002491 3.00E−30 Oryza sativa (indica ( ) scaffold002491 cultivar-group) 331 G1792 BZ359367 8.00E−30 Zea mays id72f11.b1 WGS-ZmaysF (JM107 adapted methyl filter 331 G1792 AC137635 2.00E−27 Oryza sativa (japonica Genomic sequence for cultivar-group) 331 G1792 gi23452024 4.00E−26 Lycopersicon transcription factor TSRF1. esculentum 331 G1792 gi1732406 2.10E−25 Nicotiana tabacum S25-XP1 DNA binding protein. 331 G1792 gi12597874 3.70E−25 Oryza sativa putative ethylene-responsive element binding 331 G1792 gi7528276 7.60E−25 Mesembryanthemum AP2-related transcription f crystallinum 331 G1792 gi24060081 1.30E−23 Oryza sativa (japonica putative ethylene cultivar-group) 331 G1792 gi8980313 1.80E−23 Catharanthus roseus AP2-domain DNA-binding protein. 331 G1792 gi8809571 1.80E−23 Nicotiana sylvestris ethylene-responsive element binding 331 G1792 gi17385636 1.20E−21 Matricaria chamomilla ethylene-responsive element binding 331 G1792 gi21304712 3.10E−21 Glycine max ethylene-responsive element binding protein 1 331 G1792 gi8571476 1.10E−20 Atriplex hortensis apetala2 domain-containing protein. 333 G1793 CA783156 1.00E−121 Glycine max sat20d05.y1 Gm-c1036 Glycine max cDNA clone SOY 333 G1793 AF317904 1.00E−101 Brassica napus AP2/EREBP transcription factor BABY BOOM1 (B 333 G1793 AY109146 2.00E−99 Zea mays PCO137288 mRNA sequence. 333 G1793 AY062179 2.00E−99 Oryza sativa aintegumenta-like protein mRNA, complete cds. 333 G1793 BQ864461 4.00E−91 Lactuca sativa QGC26M12.yg.ab1 QG_ABCDI lettuce salinas Lac 333 G1793 BJ178045 8.00E−89 Physcomitrella patens BJ178045 normalized ful subsp. patens 333 G1793 BF647766 3.00E−80 Medicago truncatula NF025G09EC1F1071 Elicited cell culture 333 G1793 AJ475492 1.00E−72 Hordeum vulgare AJ475492 S00008 Hordeum vulgare cDNA clone 333 G1793 BQ625052 9.00E−69 Citrus sinensis USDA-FP_02143 Ridge pineapple sweet orange 333 G1793 BJ312281 5.00E−65 Triticum aestivum BJ312281 Y. Ogihara unpublished cDNA libr 333 G1793 gi20161013 1.00E−107 Oryza sativa (japonica putative ovule dev cultivar-group) 333 G1793 gi21069053 5.70E−107 Brassica napus AP2/EREBP transcription factor BABY BOOM2. 333 G1793 gi21304227 1.50E−106 Oryza sativa ovule development aintegumenta-like protein 333 G1793 gi2652938 6.10E−97 Zea mays orf. 333 G1793 gi13173164 5.60E−45 Pisum sativum APETAL2-like protein. 333 G1793 gi11181612 3.20E−43 Picea abies APETALA2-related transcription factor 2. 333 G1793 gi18476518 9.40E−43 Hordeum vulgare APETALA2-like protein. 333 G1793 gi5081555 1.40E−41 Petunia x hybrida PHAP2A protein. 333 G1793 gi21717332 9.70E−41 Malus x domestica transcription factor AHAP2. 333 G1793 gi5360996 1.80E−34 Hyacinthus orientalis APETALA2 protein homolog HAP2. 335 G1794 BH471138 4.00E−77 Brassica oleracea BOGTX58TF BOGT Brassica oleracea genomic 335 G1794 BU873559 2.00E−36 Populus balsamifera Q056H03 Populus flow subsp. trichocarpa 335 G1794 AI485175 2.00E−36 Lycopersicon EST243479 tomato ovary, esculentum TAMU Lycope 335 G1794 BQ121959 3.00E−34 Solanum tuberosum EST607535 mixed potato tissues Solanum tu 335 G1794 AC137522 3.00E−34 Medicago truncatula clone mth2-9h8, WORKING DRAFT SEQUENCE, 335 G1794 BU763025 3.00E−31 Glycine max sas36c11.y1 Gm-c1080 Glycine max cDNA clone SOY 335 G1794 CA015575 5.00E−31 Hordeum vulgare subsp. HT14L19r HT Hordeum vulgare vulgare 335 G1794 BQ483206 6.00E−31 Triticum aestivum WHE3505_G10_M19ZS Wheat unstressed root c 335 G1794 AV428124 1.00E−30 Lotus japonicus AV428124 Lotus japonicus young plants (two- 335 G1794 AP003286 9.00E−30 Oryza sativa chromosome 1 clone P0677H08, *** SEQUENCING IN 335 G1794 gi20160854 4.80E−39 Oryza sativa (japonica hypothetical prote cultivar-group) 335 G1794 gi21740822 2.50E−26 Oryza sativa OSJNBa0042L16.10. 335 G1794 gi10798644 9.80E−25 Nicotiana tabacum AP2 domain-containing transcription fac 335 G1794 gi3342211 3.30E−24 Lycopersicon Pti4. esculentum 335 G1794 gi8809575 2.60E−23 Nicotiana sylvestris ethylene-responsive element binding 335 G1794 gi24940524 3.10E−23 Triticum aestivum ethylene response element binding prote 335 G1794 gi24817250 9.50E−23 Cicer arietinum transcription factor EREBP- like protein. 335 G1794 gi3264767 1.70E−22 Prunus armeniaca AP2 domain containing protein. 335 G1794 gi20340233 1.70E−22 Thellungiella halophila ethylene responsive element bindi 335 G1794 gi21908036 3.80E−22 Zea mays DRE binding factor 1. 337 G1804 BH496021 7.00E−87 Brassica oleracea BOGJA54TR BOGJ Brassica oleracea genomic 337 G1804 AF001453 2.00E−84 Helianthus annuus Dc3 promoter-binding factor-1 (DPBF-1) mR 337 G1804 AF519804 5.00E−53 Triticum aestivum ABA response element binding factor (ABFB 337 G1804 AP003287 8.00E−53 Oryza sativa chromosome 1 clone P0679C12, *** SEQUENCING IN 337 G1804 AAAA01001410 8.00E−53 Oryza sativa (indica ( ) scaffold001410 cultivar-group) 337 G1804 VV1237992 7.00E−48 Vitis vinifera mRNA for putative ripening-related bZIP pro 337 G1804 AF369792 2.00E−46 Phaseolus vulgaris bZIP transcription factor 6 mRNA, comple 337 G1804 AB063648 2.00E−40 Nicotiana tabacum mRNA for phi-2, complete cds. 337 G1804 AP006057 9.00E−40 Oryza sativa (japonica ( ) chromosome 9 clo cultivar-group) 337 G1804 AY110385 1.00E−38 Zea mays CL940_-1 mRNA sequence. 337 G1804 gi2228771 1.40E−78 Helianthus annuus Dc3 promoter-binding factor-1. 337 G1804 gi20161640 4.90E−47 Oryza sativa (japonica putative abscisic cultivar-group) 337 G1804 gi21693585 9.10E−43 Triticum aestivum ABA response element binding factor. 337 G1804 gi7406677 2.30E−40 Vitis vinifera putative ripening-related bZIP protein. 337 G1804 gi13775111 2.40E−37 Phaseolus vulgaris bZIP transcription factor 6. 337 G1804 gi5821255 8.00E−35 Oryza sativa TRAB1. 337 G1804 gi14571808 3.10E−26 Nicotiana tabacum phi-2. 337 G1804 gi1060935 1.30E−07 Zea mays mLIP15. 337 G1804 gi2104677 1.70E−07 Vicia faba transcription factor. 337 G1804 gi6018699 2.20E−07 Lycopersicon THY5 protein. esculentum 339 G1818 BM065544 2.00E−29 Capsicum annuum KS07004F12 KS07 Capsicum annuum cDNA, mRNA 339 G1818 BU819346 7.00E−29 Populus tremula UA42BPF01 Populus tremula cambium cDNA libr 339 G1818 AW776719 2.00E−28 Medicago truncatula EST335784 DSIL Medicago truncatula cDNA 339 G1818 BG591677 5.00E−28 Solanum tuberosum EST499519 P. infestans- challenged leaf So 339 G1818 BI321875 3.00E−27 Glycine max saf52e11.y3 Gm-c1077 Glycine max cDNA clone GEN 339 G1818 BE208917 1.00E−26 Citrus x paradisi GF-FV-P3F5 Marsh grapefruit young flavedo 339 G1818 BG440805 1.00E−26 Gossypium arboreum GA_Ea0010D12f Gossypium arboreum 7-10 d 339 G1818 BU582324 4.00E−26 Zea mays 946188B03.y1 946-tassel primordium prepared by S 339 G1818 BI127986 4.00E−26 Populus tremula x G069P33Y Populus camb Populus tremuloides 339 G1818 AW093662 4.00E−26 Lycopersicon EST286842 tomato mixed esculentum elicitor, BT 339 G1818 gi5257260 4.60E−27 Oryza sativa Similar to sequence of BAC F7G19 from Arabid 339 G1818 gi20804442 4.40E−13 Oryza sativa (japonica hypothetical prote cultivar-group) 339 G1818 gi18481626 2.60E−07 Zea mays repressor protein. 339 G1818 gi169195 0.95 Petunia x hybrida Major Cab protein. 339 G1818 gi1262851 0.98 Pinus palustris type 2 light-harvesting chlorophyll a/b-b 339 G1818 gi22536010 0.99 Phaseolus coccineus LEC1-like protein 341 G1820 AW776719 1.00E−43 Medicago truncatula EST335784 DSIL Medicago truncatula cDNA 341 G1820 BM065544 3.00E−40 Capsicum annuum KS07004F12 KS07 Capsicum annuum cDNA, mRNA 341 G1820 BG591677 4.00E−40 Solanum tuberosum EST499519 P. infestans- challenged leaf So 341 G1820 BI701620 1.00E−38 Glycine max sai18a04.y1 Gm-c1053 Glycine max cDNA clone GEN 341 G1820 BQ411597 3.00E−37 Gossypium arboreum GA_Ed0041B06f Gossypium arboreum 7-10 d 341 G1820 BE208917 6.00E−37 Citrus x paradisi GF-FV-P3F5 Marsh grapefruit young flavedo 341 G1820 BH725354 1.00E−36 Brassica oleracea BOHVO37TF BO_2_3_KB Brassica oleracea gen 341 G1820 AW093662 9.00E−36 Lycopersicon EST286842 tomato mixed esculentum elicitor, BT 341 G1820 BU819346 4.00E−35 Populus tremula UA42BPF01 Populus tremula cambium cDNA libr 341 G1820 AAAA01002977 3.00E−34 Oryza sativa (indica ( ) scaffold002977 cultivar-group) 341 G1820 gi5257260 1.40E−34 Oryza sativa Similar to sequence of BAC F7G19 from Arabid 341 G1820 gi20804442 1.70E−15 Oryza sativa (japonica hypothetical prote cultivar-group) 341 G1820 gi18481626 6.30E−08 Zea mays repressor protein. 341 G1820 gi297871 0.39 Picea abies histone H2A. 341 G1820 gi297887 0.41 Daucus carota glycine rich protein. 341 G1820 gi2130105 0.54 Triticum aestivum histone H2A.4-wheat. 341 G1820 gi6782438 0.74 Nicotiana glauca glycine-rich protein. 341 G1820 gi15214035 0.98 Cicer arietinum HISTONE H2A. 341 G1820 gi2317760 0.98 Pinus taeda H2A homolog. 341 G1820 gi1173628 0.99 Phalaenopsis sp. glycine-rich protein. SM9108 343 G1836 BI701620 7.00E−35 Glycine max sai18a04.y1 Gm-c1053 Glycine max cDNA clone GEN 343 G1836 AW776719 2.00E−33 Medicago truncatula EST335784 DSIL Medicago truncatula cDNA 343 G1836 BQ411597 2.00E−33 Gossypium arboreum GA_Ed0041B06f Gossypium arboreum 7-10 d 343 G1836 BM065544 2.00E−32 Capsicum annuum KS07004F12 KS07 Capsicum annuum cDNA, mRNA 343 G1836 BG591677 3.00E−31 Solanum tuberosum EST499519 P. infestans- challenged leaf So 343 G1836 BU819346 6.00E−31 Populus tremula UA42BPF01 Populus tremula cambium cDNA libr 343 G1836 BH725354 4.00E−30 Brassica oleracea BOHVO37TF BO_2_3_KB Brassica oleracea gen 343 G1836 BE208917 6.00E−30 Citrus x paradisi GF-FV-P3F5 Marsh grapefruit young flavedo 343 G1836 AAAA01024926 5.00E−29 Oryza sativa (indica ( ) scaffold024926 cultivar-group) 343 G1836 AW093662 9.00E−29 Lycopersicon EST286842 tomato mixed esculentum elicitor, BT 343 G1836 gi5257260 2.10E−29 Oryza sativa Similar to sequence of BAC F7G19 from Arabid 343 G1836 gi20804442 6.30E−16 Oryza sativa (japonica hypothetical prote cultivar-group) 343 G1836 gi18481626 2.00E−06 Zea mays repressor protein. 343 G1836 gi18539425 0.84 Pinus sylvestris putative malate dehydrogenase. 343 G1836 gi122084 1 Hordeum vulgare Histone H3. 343 G1836 gi225348 1 Hordeum vulgare subsp. histone H3. vulgare 345 G1838 AF317904 2.00E−98 Brassica napus AP2/EREBP transcription factor BABY BOOM1 (B 345 G1838 CA783156 7.00E−97 Glycine max sat20d05.y1 Gm-c1036 Glycine max cDNA clone SOY 345 G1838 AY109146 6.00E−96 Zea mays PCO137288 mRNA sequence. 345 G1838 AY062179 2.00E−93 Oryza sativa aintegumenta-like protein mRNA, complete cds. 345 G1838 BJ178045 3.00E−84 Physcomitrella patens BJ178045 normalized ful subsp. patens 345 G1838 BQ864461 2.00E−83 Lactuca sativa QGC26M12.yg.ab1 QG_ABCDI lettuce salinas Lac 345 G1838 BF647766 5.00E−73 Medicago truncatula NF025G09EC1F1071 Elicited cell culture 345 G1838 AJ475492 3.00E−69 Hordeum vulgare AJ475492 S00008 Hordeum vulgare cDNA clone 345 G1838 BQ625052 6.00E−69 Citrus sinensis USDA-FP_02143 Ridge pineapple sweet orange 345 G1838 BJ312281 4.00E−60 Triticum aestivum BJ312281 Y. Ogihara unpublished cDNA libr 345 G1838 gi21069051 3.00E−100 Brassica napus AP2/EREBP transcription factor BABY BOOM1. 345 G1838 gi21304225 1.30E−95 Oryza sativa aintegumenta-like protein. 345 G1838 gi20161013 3.00E−91 Oryza sativa (japonica putative ovule dev cultivar-group) 345 G1838 gi2652938 2.50E−90 Zea mays orf. 345 G1838 gi13173164 1.10E−51 Pisum sativum APETAL2-like protein. 345 G1838 gi21717332 1.10E−46 Malus x domestica transcription factor AHAP2. 345 G1838 gi5081557 1.50E−44 Petunia x hybrida PHAP2B protein. 345 G1838 gi18476518 9.40E−43 Hordeum vulgare APETALA2-like protein. 345 G1838 gi11181612 9.90E−42 Picea abies APETALA2-related transcription factor 2. 345 G1838 gi5360996 3.60E−34 Hyacinthus orientalis APETALA2 protein homolog HAP2. 347 G1841 BI421895 2.00E−37 Lycopersicon EST532561 tomato callus, esculentum TAMU Lycop 347 G1841 BU873559 3.00E−36 Populus balsamifera Q056H03 Populus flow subsp. trichocarpa 347 G1841 AC120527 6.00E−35 Oryza sativa chromosome 11 clone OSJNBa0011J22, *** SEQUENC 347 G1841 AAAA01002409 2.00E−34 Oryza sativa (indica ( ) scaffold002409 cultivar-group) 347 G1841 BE429439 7.00E−34 Triticum aestivum TAS000.B08R990618 ITEC TAS Wheat cDNA Lib 347 G1841 AW685799 2.00E−32 Medicago truncatula NF030D09NR1F1000 Nodulated root Medicag 347 G1841 BE494041 5.00E−32 Secale cereale WHE1277_B09_D17ZS Secale cereale anther cDNA 347 G1841 BU763025 1.00E−31 Glycine max sas36c11.y1 Gm-c1080 Glycine max cDNA clone SOY 347 G1841 CA015575 4.00E−31 Hordeum vulgare subsp. HT14L19r HT Hordeum vulgare vulgare 347 G1841 AV428124 8.00E−30 Lotus japonicus AV428124 Lotus japonicus young plants (two- 347 G1841 gi20160854 4.00E−37 Oryza sativa (japonica hypothetical prote cultivar-group) 347 G1841 gi10798644 2.80E−27 Nicotiana tabacum AP2 domain-containing transcription fac 347 G1841 gi21740822 2.50E−26 Oryza sativa OSJNBa0042L16.10. 347 G1841 gi22074046 4.20E−24 Lycopersicon transcription factor JERF1. esculentum 347 G1841 gi24817250 4.20E−24 Cicer arietinum transcription factor EREBP- like protein. 347 G1841 gi1688233 5.40E−24 Solanum tuberosum DNA binding protein homolog. 347 G1841 gi3264767 1.10E−23 Prunus armeniaca AP2 domain containing protein. 347 G1841 gi18496063 3.00E−23 Fagus sylvatica ethylene responsive element binding prote 347 G1841 gi24940524 1.00E−22 Triticum aestivum ethylene response element binding prote 347 G1841 gi20340233 2.70E−22 Thellungiella halophila ethylene responsive element bindi 349 G1842 AY036888 5.00E−56 Brassica napus MADS-box protein (FLC1) mRNA, complete cds. 349 G1842 BG544805 2.00E−37 Brassica rapa subsp. E2809 Chinese cabbage pekinensis etiol 349 G1842 BG596731 7.00E−36 Solanum tuberosum EST495409 cSTS Solanum tuberosum cDNA clo 349 G1842 AW219962 9.00E−36 Lycopersicon EST302445 tomato root esculentum during/after 349 G1842 BM436799 4.00E−34 Vitis vinifera VVA010B05_53181 An expressed sequence tag da 349 G1842 BQ868455 2.00E−30 Lactuca sativa QGD14A13.yg.ab1 QG_ABCDI lettuce salinas Lac 349 G1842 BI957545 1.00E−29 Hordeum vulgare HVSMEn0010B09f Hordeum vulgare rachis EST 1 349 G1842 BJ213269 2.00E−29 Triticum aestivum BJ213269 Y. Ogihara unpublished cDNA libr 349 G1842 AI900863 4.00E−29 Glycine max sb95d06.y1 Gm-c1012 Glycine max cDNA clone GENO 349 G1842 AF112150 5.00E−29 Zea mays MADS box protein 3 (mads3) mRNA, complete cds. 349 G1842 gi17933450 4.80E−55 Brassica napus MADS-box protein. 349 G1842 gi1483232 1.10E−30 Betula pendula MADS5 protein. 349 G1842 gi9367313 1.40E−30 Hordeum vulgare MADS-box protein 8. 349 G1842 gi6469345 1.80E−30 Brassica rapa subsp. DNA-binding protein. pekinensis 349 G1842 gi12002141 3.00E−30 Zea mays MADS box protein 3. 349 G1842 gi11037010 6.30E−30 Eucalyptus globulus MADS-box protein EAP2S. 349 G1842 gi1561784 6.30E−30 Brassica oleracea homeotic protein boiCAL. 349 G1842 gi4204234 8.00E−30 Lolium temulentum MADS-box protein 2. 349 G1842 gi13446154 1.70E−29 Pisum sativum MADS-box transcription factor. 349 G1842 gi21070923 1.70E−29 Oryza sativa (japonica AP1-like MADS-box cultivar-group) 351 G1843 AY036889 5.00E−56 Brassica napus MADS-box protein (FLC2) mRNA, complete cds. 351 G1843 BG596731 3.00E−35 Solanum tuberosum EST495409 cSTS Solanum tuberosum cDNA clo 351 G1843 BG544805 4.00E−35 Brassica rapa subsp. E2809 Chinese cabbage pekinensis etiol 351 G1843 AW219962 2.00E−34 Lycopersicon EST302445 tomato root esculentum during/after 351 G1843 BM436799 4.00E−34 Vitis vinifera VVA010B05_53181 An expressed sequence tag da 351 G1843 BQ850592 4.00E−32 Lactuca sativa QGB13A16.yg.ab1 QG_ABCDI lettuce salinas Lac 351 G1843 BU875165 8.00E−32 Populus balsamifera V003A12 Populus flow subsp. trichocarpa 351 G1843 BU887610 9.00E−31 Populus tremula x R064B01 Populus root Populus tremuloides 351 G1843 AF035379 3.00E−30 Lolium temulentum MADS-box protein 2 (MADS2) mRNA, alternat 351 G1843 AY040247 6.00E−30 Antirrhinum majus MADS-box transcription factor DEFH28 mRNA 351 G1843 gi17933452 2.30E−55 Brassica napus MADS-box protein. 351 G1843 gi21070923 7.80E−32 Oryza sativa (japonica AP1-like MADS-box cultivar-group) 351 G1843 gi16874557 1.60E−31 Antirrhinum majus MADS-box transcription factor DEFH28. 351 G1843 gi4204234 2.60E−31 Lolium temulentum MADS-box protein 2. 351 G1843 gi7592642 2.60E−31 Oryza sativa AP1-like MADS box protein. 351 G1843 gi9367313 7.00E−31 Hordeum vulgare MADS-box protein 8. 351 G1843 gi3688589 4.90E−30 Triticum aestivum MADS box transcription factor. 351 G1843 gi6467974 1.00E−29 Dendrobium grex MADS box protein Madame Thong-In DOMADS2. 351 G1843 gi1483232 1.30E−29 Betula pendula MADS5 protein. 351 G1843 gi13384068 1.70E−29 Petunia x hybrida MADS-box transcription factor FBP29. 353 G1852 AAAA01018591 1.00E−135 Oryza sativa (indica ( ) scaffold018591 cultivar-group) 353 G1852 AF220204 1.00E−129 Malus domestica unknown mRNA. 353 G1852 BQ507509 1.00E−119 Solanum tuberosum EST614924 Generation of a set of potato c 353 G1852 BM412458 1.00E−114 Lycopersicon EST586785 tomato breaker esculentum fruit Lyco 353 G1852 AY104480 1.00E−113 Zea mays PCO099563 mRNA sequence. 353 G1852 BG581705 1.00E−108 Medicago truncatula EST483440 GVN Medicago truncatula cDNA 353 G1852 BF009089 1.00E−102 Glycine max ss73d04.y1 Gm-c1062 Glycine max cDNA clone GENO 353 G1852 AC087192 1.00E−101 Oryza sativa chromosome 10 clone OSJNBa0005K07, *** SEQUENC 353 G1852 BU013091 1.00E−100 Lactuca sativa QGJ3L13.yg.ab1 QG_EFGHJ lettuce serriola Lac 353 G1852 BG445922 9.00E−99 Gossypium arboreum GA_Ea0030A23f Gossypium arboreum 7-10 d 353 G1852 gi24413975 8.10E−124 Oryza sativa (japonica hypothetical prote cultivar-group) 353 G1852 gi6752888 2.70E−123 Malus x domestica unknown. 353 G1852 gi18071395 1.20E−122 Oryza sativa hypothetical protein. 353 G1852 gi18419598 1.30E−22 Narcissus putative methyltransferase pseudonarcissus prot 353 G1852 gi20218829 6.60E−16 Pinus pinaster hypothetical protein. 353 G1852 gi15144514 0.089 Lycopersicon unknown. esculentum 353 G1852 gi498042 0.23 Senecio odorus ORF. 353 G1852 gi4432741 0.69 Dioscorea tenuipes phosphoglucose isomerase. 353 G1852 gi1399380 0.81 Glycine max S-adenosyl-L- methionine:delta24-sterol-C- meth 355 G1863 BH582941 4.00E−61 Brassica oleracea BOHOL42TF BOHO Brassica oleracea genomic 355 G1863 AF201895 1.00E−34 Oryza sativa growth-regulating factor 1 (GRF1) mRNA, comple 355 G1863 BM404872 2.00E−34 Solanum tuberosum EST579199 potato roots Solanum tuberosum 355 G1863 AW981431 8.00E−34 Medicago truncatula EST392584 DSIL Medicago truncatula cDNA 355 G1863 BI786182 1.00E−33 Glycine max sai33h05.y1 Gm-c1065 Glycine max cDNA clone GEN 355 G1863 BQ852906 3.00E−33 Lactuca sativa QGB19E24.yg.ab1 QG_ABCDI lettuce salinas Lac 355 G1863 AW442227 1.00E−32 Lycopersicon EST311623 tomato fruit red esculentum ripe, TA 355 G1863 CA029723 3.00E−32 Hordeum vulgare subsp. HX05A15r HX Hordeum vulgare vulgare 355 G1863 AP005538 6.00E−32 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 355 G1863 AAAA01004865 1.00E−31 Oryza sativa (indica ( ) scaffold004865 cultivar-group) 355 G1863 gi6573149 1.90E−39 Oryza sativa growth-regulating factor 1. 355 G1863 gi18390099 1.20E−37 Sorghum bicolor putative growth-regulating factor 1. 355 G1863 gi24413958 1.20E−33 Oryza sativa (japonica putative growth-re cultivar-group) 355 G1863 gi19171209 0.12 Lycopersicon viroid RNA-binding protein. esculentum 355 G1863 gi7008009 0.67 Pisum sativum PsAD1. 355 G1863 gi1061308 0.79 Zea mays Dof3 gene. 355 G1863 gi2129829 0.96 Glycine max heat shock transcription factor HSF29-soybe 355 G1863 gi4680184 0.99 Oryza sativa (indica unknown. cultivar-group) 355 G1863 gi12655953 1 Brassica rapa luminidependens. 355 G1863 gi3790264 1 Triticum aestivum PBF protein. 357 G1880 BI265111 1.00E−75 Medicago truncatula NF078A11IN1F1085 Insect herbivory Medic 357 G1880 BJ192201 8.00E−75 Physcomitrella patens BJ192201 normalized ful subsp. patens 357 G1880 BH714361 3.00E−73 Brassica oleracea BOMMJ59TR BO_2_3_KB Brassica oleracea gen 357 G1880 BI972592 1.00E−71 Glycine max sai80b06.y1 Gm-c1065 Glycine max cDNA clone GEN 357 G1880 AP005381 2.00E−71 Oryza sativa (japonica ( ) chromosome 8 clo cultivar-group) 357 G1880 AAAA01002232 2.00E−69 Oryza sativa (indica ( ) scaffold002232 cultivar-group) 357 G1880 BM063853 4.00E−61 Capsicum annuum KS01060C10 KS01 Capsicum annuum cDNA, mRNA 357 G1880 BU039744 1.00E−60 Prunus persica PP_LEa0003M02f Peach developing fruit mesoca 357 G1880 BM407709 3.00E−60 Solanum tuberosum EST582036 potato roots Solanum tuberosum 357 G1880 BF050813 7.00E−60 Lycopersicon EST435971 tomato esculentum developing/immatur 357 G1880 gi9858780 1.50E−58 Lycopersicon BAC19.12. esculentum 357 G1880 gi10934090 1.20E−57 Oryza sativa putative zinc finger protein. 357 G1880 gi563623 2.20E−57 Solanum tuberosum putative DNA/RNA binding protein. 357 G1880 gi3170601 3.30E−57 Zea mays zinc finger protein ID1. 357 G1880 gi20160482 1.40E−56 Oryza sativa (japonica zinc finger protei cultivar-group) 357 G1880 gi18376601 4.40E−12 Glycine max WIP1 protein. 357 G1880 gi2346988 0.059 Petunia x hybrida ZPT4-4. 357 G1880 gi1076538 0.1 Pisum sativum gibberellin-responsive ovarian protein G14 357 G1880 gi3129939 0.81 Cicer arietinum hypothetical protein. 357 G1880 gi12585428 0.91 Nicotiana tabacum Vacuolar ATP synthase subunit G 1 (V-AT 359 G1895 BH418383 5.00E−94 Brassica oleracea BOHQS10TR BOHQ Brassica oleracea genomic 359 G1895 AC073556 7.00E−35 Oryza sativa chromosome unknown clone OSJNBa0091P11, *** SE 359 G1895 D45066 3.00E−34 Cucurbita maxima mRNA for AOBP (ascorbate oxidase promoter- 359 G1895 BQ488386 2.00E−33 Beta vulgaris 43-E8885-006-003-F11-T3 Sugar beet MPIZ-ADIS- 359 G1895 BF649498 9.00E−33 Medicago truncatula NF079C08EC1F1065 Elicited cell culture 359 G1895 BQ860203 3.00E−32 Lactuca sativa QGC15B22.yg.ab1 QG_ABCDI lettuce salinas Lac 359 G1895 HVU312330 4.00E−32 Hordeum vulgare subsp. Hordeum vulgare partial dof vulgare 359 G1895 AW931465 3.00E−31 Lycopersicon EST357308 tomato fruit esculentum mature green 359 G1895 AAAA01007635 4.00E−31 Oryza sativa (indica ( ) scaffold007635 cultivar-group) 359 G1895 CA783807 1.00E−30 Glycine max sat57f01.y1 Gm-c1056 Glycine max cDNA clone SOY 359 G1895 gi19071625 1.80E−41 Oryza sativa (japonica putative zinc fing cultivar-group) 359 G1895 gi7242908 1.40E−40 Oryza sativa ESTs C23582(S11122), AU056531 (S20663) corresp 359 G1895 gi1669341 1.50E−39 Cucurbita maxima AOBP (ascorbate oxidase promoter-binding 359 G1895 gi21538791 4.90E−30 Hordeum vulgare subsp. dof zinc finger protein. vulgare 359 G1895 gi3929325 6.90E−24 Dendrobium grex putative DNA-binding prot Madame Thong-In 359 G1895 gi1360078 1.10E−23 Nicotiana tabacum Zn finger protein. 359 G1895 gi6092016 1.30E−22 Pisum sativum elicitor-responsive Dof protein ERDP. 359 G1895 gi7688355 3.40E−22 Solanum tuberosum Dof zinc finger protein. 359 G1895 gi1061306 9.00E−22 Zea mays Dof2. 359 G1895 gi3790264 3.90E−21 Triticum aestivum PBF protein. 361 G1902 BH516623 8.00E−87 Brassica oleracea BOGHO31TR BOGH Brassica oleracea genomic 361 G1902 BE610227 3.00E−40 Glycine max sq51e07.y1 Gm-c1019 Glycine max cDNA clone GENO 361 G1902 BE433484 2.00E−38 Lycopersicon EST400013 tomato breaker esculentum fruit, TIG 361 G1902 BQ790994 3.00E−38 Brassica rapa subsp. E4860 Chinese cabbage pekinensis etiol 361 G1902 BQ505729 3.00E−37 Solanum tuberosum EST613144 Generation of a set of potato c 361 G1902 BG454338 1.00E−35 Medicago truncatula NF113E12LF1F1088 Developing leaf Medica 361 G1902 BU832216 1.00E−31 Populus tremula x T030H07 Populus apica Populus tremuloides 361 G1902 BM066503 6.00E−30 Capsicum annuum KS07015B04 KS07 Capsicum annuum cDNA, mRNA 361 G1902 AC133003 7.00E−30 Oryza sativa (japonica ( ) chromosome 3 clo cultivar-group) 361 G1902 AW398140 1.00E−29 Lycopersicon pennellii EST298023 L. pennellii trichome, Cor 361 G1902 gi4996640 3.70E−31 Oryza sativa Dof zinc finger protein. 361 G1902 gi3341468 1.80E−30 Nicotiana tabacum Dof zinc finger protein. 361 G1902 gi3790264 2.60E−30 Triticum aestivum PBF protein. 361 G1902 gi19387252 3.30E−30 Oryza sativa (japonica putative zinc-fing cultivar-group) 361 G1902 gi21538793 1.30E−29 Hordeum vulgare subsp. dof zinc finger protein. vulgare 361 G1902 gi3777436 1.80E−29 Hordeum vulgare DNA binding protein. 361 G1902 gi6092016 2.10E−29 Pisum sativum elicitor-responsive Dof protein ERDP. 361 G1902 gi1061308 6.60E−28 Zea mays Dof3 gene. 361 G1902 gi7688355 1.70E−27 Solanum tuberosum Dof zinc finger protein. 361 G1902 gi1669341 1.00E−22 Cucurbita maxima AOBP (ascorbate oxidase promoter-binding 363 G1903 BH590326 1.00E−111 Brassica oleracea BOGGK32TR BOGG Brassica oleracea genomic 363 G1903 AC073556 2.00E−41 Oryza sativa chromosome unknown clone OSJNBa0091P11, *** SE 363 G1903 D45066 1.00E−39 Cucurbita maxima mRNA for AOBP (ascorbate oxidase promoter- 363 G1903 HVU312330 3.00E−37 Hordeum vulgare subsp. Hordeum vulgare partial dof vulgare 363 G1903 AP005167 3.00E−35 Oryza sativa (japonica ( ) chromosome 7 clo cultivar-group) 363 G1903 BQ860203 3.00E−35 Lactuca sativa QGC15B22.yg.ab1 QG_ABCDI lettuce salinas Lac 363 G1903 BI934963 3.00E−35 Lycopersicon EST554852 tomato flower, esculentum anthesis L 363 G1903 AAAA01004298 4.00E−35 Oryza sativa (indica ( ) scaffold004298 cultivar-group) 363 G1903 BF649498 9.00E−35 Medicago truncatula NF079C08EC1F1065 Elicited cell culture 363 G1903 CA484955 2.00E−33 Triticum aestivum WHE4312_F07_L14ZS Wheat meiotic anther cD 363 G1903 gi19071625 6.70E−47 Oryza sativa (japonica putative zinc fing cultivar-group) 363 G1903 gi7242908 3.20E−43 Oryza sativa ESTs C23582(S11122), AU056531 (S20663) corresp 363 G1903 gi1669341 8.50E−43 Cucurbita maxima AOBP (ascorbate oxidase promoter-binding 363 G1903 gi21538791 1.90E−39 Hordeum vulgare subsp. dof zinc finger protein. vulgare 363 G1903 gi1360084 6.20E−26 Nicotiana tabacum Zn finger protein. 363 G1903 gi3790264 9.20E−26 Triticum aestivum PBF protein. 363 G1903 gi2393775 7.10E−25 Zea mays prolamin box binding factor. 363 G1903 gi7688355 4.20E−24 Solanum tuberosum Dof zinc finger protein. 363 G1903 gi6092016 5.50E−24 Pisum sativum elicitor-responsive Dof protein ERDP. 363 G1903 gi3929325 9.50E−24 Dendrobium grex putative DNA-binding prot Madame Thong-In 365 G1919 BH997456 3.00E−39 Brassica oleracea oef07e04.b1 B. oleracea002 Brassica olerac 365 G1919 AP005090 7.00E−28 Oryza sativa (japonica ( ) chromosome 9 clo cultivar-group) 365 G1919 AAAA01013304 2.00E−27 Oryza sativa (indica ( ) scaffold013304 cultivar-group) 365 G1919 AC126012 3.00E−26 Medicago truncatula clone mth2-27p4, WORKING DRAFT SEQUENCE 365 G1919 NPY09105 1.00E−23 Nicotiana N. plumbaginifolia mRNA plumbaginifolia for unknow 365 G1919 BU000353 1.00E−22 Lactuca sativa QGG24J16.yg.ab1 QG_EFGHJ lettuce serriola La 365 G1919 AV914826 1.00E−20 Hordeum vulgare subsp. AV914826 K. Sato vulgare unpublished 365 G1919 AW704699 4.00E−18 Glycine max sk39d07.y1 Gm-c1028 Glycine max cDNA clone GENO 369 G1930 AC135925 7.00E−79 Oryza sativa (japonica ( ) chromosome 5 clo cultivar-group) 369 G1930 AAAA01000997 3.00E−78 Oryza sativa (indica ( ) scaffold000997 cultivar-group) 369 G1930 BU994579 1.00E−65 Hordeum vulgare subsp. HM07I08r HM Hordeum vulgare vulgare 369 G1930 BQ405698 1.00E−65 Gossypium arboreum GA_Ed0085H02f Gossypium arboreum 7-10 d 369 G1930 BF520598 1.00E−64 Medicago truncatula EST458071 DSIL Medicago truncatula cDNA 369 G1930 BZ015521 1.00E−64 Brassica oleracea oeg86a05.g1 B. oleracea002 Brassica olerac 369 G1930 BF424857 2.00E−58 Glycine max su59h03.y1 Gm-c1069 Glycine max cDNA clone GENO 369 G1930 BU870896 1.00E−56 Populus balsamifera Q019F06 Populus flow subsp. trichocarpa 369 G1930 gi18565433 4.10E−74 Oryza sativa (japonica DNA-binding protei cultivar-group) 369 G1930 gi12328560 1.80E−71 Oryza sativa putative DNA binding protein RAV2. 369 G1930 gi10798644 1.40E−13 Nicotiana tabacum AP2 domain-containing transcription fac 369 G1930 gi20340233 5.10E−11 Thellungiella halophila ethylene responsive element bindi 369 G1930 gi4099921 1.30E−10 Stylosanthes hamata EREBP-3 homolog. 369 G1930 gi18496063 1.60E−10 Fagus sylvatica ethylene responsive element binding prote 369 G1930 gi22074046 2.10E−10 Lycopersicon transcription factor JERF1. esculentum 369 G1930 gi3264767 2.30E−10 Prunus armeniaca AP2 domain containing protein. 369 G1930 gi18266198 1.10E−09 Narcissus AP-2 domain containing pseudonarcissus protein. 369 G1930 gi24940524 1.10E−09 Triticum aestivum ethylene response element binding prote 371 G1936 AX540653 1.00E−139 Zea mays Sequence 9 from Patent WO0240688. 371 G1936 BH735681 4.00E−45 Brassica oleracea BOHTG58TR BO_2_3_KB Brassica oleracea gen 371 G1936 AW279046 2.00E−28 Glycine max sg07b03.y1 Gm-c1019 Glycine max cDNA clone GENO 371 G1936 BQ874162 2.00E−26 Lactuca sativa QGI4J06.yg.ab1 QG_ABCDI lettuce salinas Lact 371 G1936 BG645784 5.00E−26 Medicago truncatula EST507403 KV3 Medicago truncatula cDNA 371 G1936 AP004223 5.00E−26 Oryza sativa (japonica ( ) genomic DNA, chr cultivar-group) 371 G1936 AW219090 2.00E−23 Lycopersicon EST301572 tomato root esculentum during/after 371 G1936 BQ118395 5.00E−23 Solanum tuberosum EST603971 mixed potato tissues Solanum tu 371 G1936 CA816557 6.00E−23 Vitis vinifera CA12EI303IVF_H11 Cabernet Sauvignon Leaf-C 371 G1936 BG445379 3.00E−22 Gossypium arboreum GA_Ea0027O21f Gossypium arboreum 7-10 d 371 G1936 gi20975251 7.50E−23 Oryza sativa (japonica transcription fact cultivar-group) 371 G1936 gi2580440 2.20E−21 Oryza sativa PCF2. 371 G1936 gi5731257 5.00E−21 Gossypium hirsutum auxin-induced basic helix- loop-helix t 371 G1936 gi6358622 0.00035 Digitalis purpurea cyc4 protein. 371 G1936 gi6358625 0.00035 Misopates orontium cyc4 protein. 371 G1936 gi6358621 0.00061 Antirrhinum majus cyc4 protein. subsp. cirrhigerum 371 G1936 gi6358623 0.00061 Antirrhinum graniticum cyc4 protein. 371 G1936 gi6466188 0.00085 Antirrhinum majus flower asymmetry protein DICHOTOMA. 371 G1936 gi12002867 0.0036 Lycopersicon cycloidea. esculentum 371 G1936 gi6358551 0.0092 Antirrhinum majus cyc1A protein. subsp. linkianum 373 G1944 BU926769 1.00E−86 Glycine max sas91d09.y1 Gm-c1036 Glycine max cDNA clone SOY 373 G1944 BU814921 8.00E−73 Populus tremula x N034H11 Populus bark Populus tremuloides 373 G1944 BG589060 8.00E−70 Medicago truncatula EST490869 MHRP- Medicago truncatula cDN 373 G1944 BG441060 1.00E−64 Gossypium arboreum GA_Ea0011I19f Gossypium arboreum 7-10 d 373 G1944 BI139442 3.00E−64 Populus balsamifera F131P74Y Populus flo subsp. trichocarpa 373 G1944 BG643949 5.00E−59 Lycopersicon EST512143 tomato esculentum shoot/meristem Lyc 373 G1944 AU289368 2.00E−58 Zinnia elegans AU289368 zinnia cultured mesophyll cell equa 373 G1944 BQ868100 3.00E−52 Lactuca sativa QGD13A19.yg.ab1 QG_ABCDI lettuce salinas Lac 373 G1944 BU892499 2.00E−50 Populus tremula P064F04 Populus petioles cDNA library Popul 373 G1944 AV425818 1.00E−48 Lotus japonicus AV425818 Lotus japonicus young plants (two- 373 G1944 gi12643044 7.80E−58 Oryza sativa putative AT-Hook DNA- binding protein. 373 G1944 gi2213536 4.40E−45 Pisum sativum DNA-binding protein PD1. 373 G1944 gi4165183 3.20E−41 Antirrhinum majus SAP1 protein. 373 G1944 gi24418033 4.50E−15 Oryza sativa (japonica Hypothetical prote cultivar-group) 373 G1944 gi100212 0.0032 Lycopersicon extensin class II (clones esculentum u1/u2) 373 G1944 gi167556 0.016 Daucus carota extensin. 373 G1944 gi555655 0.035 Nicotiana tabacum DNA-binding protein. 373 G1944 gi72327 0.043 Zea mays glutelin 5-maize. 373 G1944 gi1076237 0.06 Pinus taeda arabinogalactan-like protein- loblolly pine. 373 G1944 gi1247390 0.076 Nicotiana alata PRP3. 375 G1946 LPHSF8 1.00E−127 Lycopersicon L. peruvianum Lp-hsf8 peruvianum mRNA for heat 375 G1946 AC087771 4.00E−96 Medicago truncatula clone 8D15, *** SEQUENCING IN PROGRESS 375 G1946 LEHSF8 300E−86 Lycopersicon L. esculentum Le-hsf8 gene esculentum for heat 375 G1946 AW569256 1.00E−84 Glycine max si64g09.y1 Gm-r1030 Glycine max cDNA clone GENO 375 G1946 AAAA01005302 7.00E−80 Oryza sativa (indica ( )scaffold0053O02 cultivar-group) 375 G1946 AC120506 1.00E−79 Oryza sativa chromosome 3 clone OSJNBb0006008, *** SEQUENCI 375 G1946 BG890899 2.00E−79 Solanum tuberosum EST516750 cSTD Solanum tuberosum cDNA clo 375 G1946 BU834690 8.00E−73 Populus tremula x T064E07 Populus apica Populus tremuloides 375 G1946 AV833112 1.00E−60 Hordeum vulgare subsp. AV833112 K. Sato vulgare unpublished 375 G1946 BQ916240 4.00E−59 Helianthus annuus QHB17D05.yg.ab1 QH_ABCDI sunflower RHA801 375 G1946 gi100264 1.90E−123 Lycopersicon heat shock transcription peruvianum factor H 375 G1946 gi100225 9.10E−109 Lycopersicon heat shock transcription esculentum factor H 375 G1946 gi24308618 5.40E−63 Oryza sativa (japonica Putative heat shoc cultivar-group) 375 G1946 gi5821138 5.40E−55 Nicotiana tabacum heat shock factor. 375 G1946 gi662924 2.10E−52 Glycine max heat shock transcription factor 21. 375 G1946 gi25052685 2.50E−51 Helianthus annuus heat stress transcription factor HSFA9. 375 G1946 gi16118447 4.80E−50 Phaseolus acutifolius heat shock transcription factor. 375 G1946 gi14209551 6.10E−48 Oryza sativa putative heat shock factor. 375 G1946 gi20162459 1.40E−46 Medicago sativa heat shock transcription factor. 375 G1946 gi1362193 3.40E−45 Zea mays heat shock factor-maize. 377 G1947 BE319312 1.00E−49 Medicago truncatula NF015D08NR1F1035 Nodulated root Medicag 377 G1947 LPHSF30 1.00E−48 Lycopersicon L. peruvianum Lp-hsf30 peruvianum mRNA for heat 377 G1947 BM086093 5.00E−48 Glycine max sah35d07.y1 Gm-c1036 Glycine max cDNA clone SOY 377 G1947 AV833112 6.00E−47 Hordeum vulgare subsp. AV833112 K. Sato vulgare unpublished 377 G1947 BI406849 2.00E−44 Solanum tuberosum 182A06 Mature tuber lambda ZAP Solanum tu 377 G1947 AY099451 2.00E−44 Helianthus annuus heat stress transcription factor HSFA9 mR 377 G1947 AW034874 8.00E−44 Lycopersicon EST279103 tomato callus, esculentum TAMU Lycop 377 G1947 AAAA01016817 1.00E−42 Oryza sativa (indica ( )scaffold016817 cultivar-group) 377 G1947 BI305378 6.00E−39 Oryza sativa NRS_2_7_8_A01_K18 Drought stress (root) Oryza 377 G1947 BI479783 4.00E−38 Triticum aestivum WHE3452_A08_A16ZS Wheat pre-anthesis spik 377 G1947 gi100265 2.10E−47 Lycopersicon heat shock transcription peruvianum factor H 377 G1947 gi2129828 8.70E−40 Glycine max heat shock transcription factor HSF21-soybe 377 G1947 gi20521264 4.30E−39 Oryza sativa (japonica putative heat shoc cultivar-group) 377 G1947 gi25052685 5.50E−38 Helianthus annuus heat stress transcription factor HSFA9. 377 G1947 gi14209551 9.30E−38 Oryza sativa putative heat shock factor. 377 G1947 gi16118447 1.20E−37 Phaseolus acutifolius heat shock transcription factor. 377 G1947 gi20162459 2.70E−36 Medicago sativa heat shock transcription factor. 377 G1947 gi5821138 4.50E−36 Nicotiana tabacum heat shock factor. 377 G1947 gi2130133 7.30E−36 Zea mays heat shock transcription factor (clone hsfa)-m 377 G1947 gi100225 2.50E−35 Lycopersicon heat shock transcription esculentum factor H 379 G1948 BG321479 1.00E−128 Descurainia sophia Ds01_07g10_A Ds01_AAFC_ECORC_cold_stress 379 G1948 BQ704285 1.00E−100 Brassica napus Bn01_04d19_A 379 G1948 AC098693 3.00E−92 Oryza sativa chromosome 3 clone OJ1004_C08, *** SEQUENCING 379 G1948 BH435688 2.00E−88 Brassica oleracea BOHHK12TF BOHH Brassica oleracea genomic 379 G1948 BI933410 4.00E−59 Lycopersicon EST553311 tomato flower, esculentum anthesis L 379 G1948 BQ511165 7.00E−58 Solanum tuberosum EST618580 Generation of a set of potato c 379 G1948 AAAA01005130 5.00E−57 Oryza sativa (indica ( ) scaffold005130 cultivar-group) 379 G1948 BU011081 9.00E−53 Lactuca sativa QGJ15D24.yg.ab1 QG_EFGHJ lettuce serriola La 379 G1948 BU031848 6.00E−39 Helianthus annuus QHJ19M09.yg.ab1 QH_EFGHJ sunflower RHA280 379 G1948 BG300992 3.00E−36 Hordeum vulgare HVSMEb0019C24f Hordeum vulgare seedling sho 379 G1948 gi20502992 5.50E−86 Oryza sativa (japonica Putative CAO prote cultivar-group) 379 G1948 gi549986 1.40E−12 Pennisetum ciliare possible apospory- associated protein. 379 G1948 gi19070767 3.10E−12 Oryza sativa apospory-associated protein. 379 G1948 gi24637568 5.50E−12 Nicotiana tabacum ankyrin domain protein. 379 G1948 gi17645766 9.80E−12 Glycine max unnamed protein product. 379 G1948 gi7110220 1.20E−06 Triticum aestivum AKT1-like potassium channel. 379 G1948 gi2104908 1.50E−05 Zea mays potassium channel. 379 G1948 gi24745936 1.70E−05 Solanum tuberosum ankyrin-like protein. 379 G1948 gi20127124 0.00014 Brassica napus calmodulin-binding transcription activator 379 G1948 gi16550932 0.00031 Eucalyptus inward-rectifying K+ camaldulensis channel. 381 G1950 BG599002 2.00E−83 Solanum tuberosum EST503902 cSTS Solanum tuberosum cDNA clo 381 G1950 BQ857787 4.00E−78 Lactuca sativa QGB8H12.yg.ab1 QG_ABCDI lettuce salinas Lact 381 G1950 AW100050 3.00E−69 Glycine max sd25e07.y1 Gm-c1012 Glycine max cDNA clone GENO 381 G1950 BF177815 4.00E−60 Lotus japonicus Ljirnpest34-724-d7 Ljirnp Lambda HybriZap t 381 G1950 BG466155 1.00E−59 Euphorbia esula 00918 leafy spurge Lambda HybriZAP 2.1 two- 381 G1950 BU820489 3.00E−55 Populus tremula UB10CPG06 Populus tremula cambium cDNA libr 381 G1950 BE443704 1.00E−50 Triticum aestivum WHE1121_C05_E09ZS Wheat etiolated seedlin 381 G1950 BG267984 3.00E−49 Zea mays 1000144D01.x1 1000- Unigene I from Maize Genome P 381 G1950 BI266915 1.00E−47 Medicago truncatula NF097B04IN1F1041 Insect herbivory Medic 381 G1950 BM412345 2.00E−46 Lycopersicon EST586672 tomato breaker esculentum fruit Lyco 381 G1950 gi15341604 1.00E−77 Oryza sativa putative ankyrin. 381 G1950 gi24745936 5.80E−21 Solanum tuberosum ankyrin-like protein. 381 G1950 gi13310811 9.00E−17 Nicotiana tabacum ankyrin-repeat protein HBP1. 381 G1950 gi7110220 1.10E−16 Triticum aestivum AKT1-like potassium channel. 381 G1950 gi21328024 4.00E−16 Oryza sativa (japonica putative AKT1-like cultivar-group) 381 G1950 gi17645764 9.90E−16 Glycine max unnamed protein product. 381 G1950 gi549986 1.60E−15 Pennisetum ciliare possible apospory- associated protein. 381 G1950 gi2104908 4.30E−15 Zea mays potassium channel. 381 G1950 gi2832781 3.70E−13 Egeria densa inward potassium channel alpha subunit. 381 G1950 gi8896127 1.20E−11 Mesembryanthemum putative potassium channel crystallinum 383 G1958 BH495974 5.00E−76 Brassica oleracea BOHHB37TF BOHH Brassica oleracea genomic 383 G1958 AB017693 7.00E−70 Nicotiana tabacum WERBP-1 mRNA, complete cds. 383 G1958 AF219972 4.00E−62 Mesembryanthemum CDPK substrate protein 1 crystallinum (csp 383 G1958 AW507631 1.00E−60 Glycine max si42c09.y1 Gm-r1030 Glycine max cDNA clone GENO 383 G1958 AW684291 6.00E−59 Medicago truncatula NF015B02NR1F1000 Nodulated root Medicag 383 G1958 BQ806133 4.00E−58 Triticum aestivum WHE3575_B11_C21ZS Wheat developing grains 383 G1958 AW030183 3.00E−56 Lycopersicon EST273438 tomato callus, esculentum TAMU Lycop 383 G1958 BQ587750 2.00E−53 Beta vulgaris E012340-024-010-G07-SP6 MPIZ-ADIS-024-leaf Be 383 G1958 AY107734 2.00E−53 Zea mays PCO065209 mRNA sequence. 383 G1958 CA516596 4.00E−51 Capsicum annuum KS09060E12 KS09 Capsicum annuum cDNA, mRNA 383 G1958 gi4519671 3.70E−66 Nicotiana tabacum transfactor. 383 G1958 gi6942190 1.50E−58 Mesembryanthemum CDPK substrate protein 1; C crystallinum 383 G1958 gi5916207 9.80E−27 Chlamydomonas regulatory protein of P- reinhardtii starvat 383 G1958 gi23306130 6.00E−13 Oryza sativa (japonica Unknown protein. cultivar-group) 383 G1958 gi15289981 8.40E−13 Oryza sativa hypothetical protein. 383 G1958 gi11177540 6.60E−10 Zea mays putative transcription factor Golden2. 383 G1958 gi1946222 0.81 Malus domestica knotted1-like homeobox protein. 383 G1958 gi15144509 0.96 Lycopersicon unknown. esculentum 383 G1958 gi2317676 0.96 Fagopyrum esculentum declined protein during seed develo 383 G1958 gi538502 0.96 Stylosanthes humilis peroxidase. 385 G2007 AF161711 9.00E−78 Pimpinella brachycarpa myb-related transcription factor mRN 385 G2007 CA783329 7.00E−75 Glycine max sat22g04.y1 Gm-c1036 Glycine max cDNA clone SOY 385 G2007 BU81821 1.00E−69 Populus tremula x UL88TH12 Populus leaf Populus tremuloides 385 G2007 AI770808 1.00E−67 Zea mays 606058F03.x2 606-Ear tissue cDNA library from Sc 385 G2007 OSA311053 2.00E−67 Oryza sativa mRNA for Myb15 protein (myb15 gene). 385 G2007 LETHM16 2.00E−66 Lycopersicon L. esculentum mRNA for esculentum myb-related t 385 G2007 BQ624834 5.00E−65 Citrus sinensis USDA-FP_01925 Ridge pineapple sweet orange 385 G2007 BU868208 2.00E−64 Populus balsamifera M112E10 Populus flow subsp. trichocarpa 385 G2007 AW685586 7.00E−64 Medicago truncatula NF032A05NR1F1000 Nodulated root Medicag 385 G2007 BQ245626 2.00E−62 Triticum aestivum TaE15022B12R TaE15 Triticum aestivum cDNA 385 G2007 gi6651292 7.50E−78 Pimpinella brachycarpa myb-related transcription factor. 385 G2007 gi23343577 4.60E−66 Oryza sativa Myb13 protein. 385 G2007 gi1430846 4.10E−65 Lycopersicon myb-related transcription esculentum factor. 385 G2007 gi19072740 5.00E−60 Zea mays typical P-type R2R3 Myb protein. 385 G2007 gi19073330 4.50E−59 Sorghum bicolor typical P-type R2R3 Myb protein. 385 G2007 gi20563 2.60E−53 Petunia x hybrida protein 1. 385 G2007 gi22638 3.00E−53 Physcomitrella patens Pp2. 385 G2007 gi13346194 1.50E−51 Gossypium hirsutum GHMYB9. 385 G2007 gi19386839 2.60E−51 Oryza sativa (japonica putative myb-relat cultivar-group) 385 G2007 gi4886264 5.90E−50 Antirrhinum majus Myb-related transcription factor mixta- 387 G2010 BH969114 2.00E−41 Brassica oleracea odg08d11.b1 B. oleracea002 Brassica olerac 387 G2010 BQ847567 1.00E−34 Lactuca sativa QGA3h03.yg.ab1 QG_ABCDI lettuce salinas Lact 387 G2010 BG525285 4.00E−34 Stevia rebaudiana 48-3 Stevia field grown leaf cDNA Stevia 387 G2010 BI928213 5.00E−34 Lycopersicon EST548102 tomato flower, esculentum 3-8 mm b 387 G2010 BU824105 8.00E−34 Populus tremula UB60BPD08 Populus tremula cambium cDNA libr 387 G2010 AMSPB1 1.00E−32 Antirrhinum majus A. majus mRNA for squamosa-promoter bindin 387 G2010 CA516258 3.00E−32 Capsicum annuum KS09055D03 KS09 Capsicum annuum cDNA, mRNA 387 G2010 BE058432 5.00E−32 Glycine max sn16a06.y1 Gm-c1016 Glycine max cDNA clone GENO 387 G2010 BG455868 6.00E−32 Medicago truncatula NF068F05PL1F1045 Phosphate starved leaf 387 G2010 BU028945 2.00E−30 Helianthus annuus QHH6J19.yg.ab1 QH_EFGHJ sunflower RHA280 387 G2010 gi1183866 2.50E−33 Antirrhinum majus squamosa-promoter binding protein 1. 387 G2010 gi5931780 1.10E−27 Zea mays SBP-domain protein 2. 387 G2010 gi8468036 2.30E−23 Oryza sativa Similar to Arabidopsis thaliana chromosome 2 387 G2010 gi9087308 1.50E−10 Mitochondrion Beta orf102a. vulgaris var. altissima 387 G2010 gi22535625 0.53 Oryza sativa (japonica hypothetical prote cultivar-group) 387 G2010 gi14597634 1 Physcomitrella patens 15_ppprot1_080_c02. 387 G2010 gi7209500 1 Brassica rapa S-locus pollen protein. 389 G2053 BH923697 3.00E−31 Brassica oleracea odi23h12.b1 B. oleracea002 Brassica olerac 389 G2053 AF532619 2.00E−25 Glycine max no apical meristem-like protein mRNA, complete 389 G2053 AF509874 2.00E−24 Petunia x hybrida nam-like protein 11 (NH11) mRNA, complete 389 G2053 BQ864249 4.00E−24 Lactuca sativa QGC26D03.yg.ab1 QG_ABCDI lettuce salinas Lac 389 G2053 BI246023 8.00E−24 Sorghum bicolor IP1_66_F11.b1_A002 Immature pannicle 1 (IP1 389 G2053 CA815703 1.00E−23 Vitis vinifera CA12EI204IVF_E10 Cabernet Sauvignon Leaf-C 389 G2053 BQ586991 1.00E−23 Beta vulgaris E012352-024-011-F06-SP6 MPIZ-ADIS-024-leaf Be 389 G2053 BF645220 1.00E−23 Medicago truncatula NF032F12EC1F1102 Elicited cell culture 389 G2053 BU894596 1.00E−23 Populus tremula x X011H04 Populus wood Populus tremuloides 389 G2053 BG543974 1.00E−23 Brassica rapa subsp. E1725 Chinese cabbage pekinensis etiol 389 G2053 gi22597158 6.50E−28 Glycine max no apical meristem-like protein. 389 G2053 gi21105736 2.00E−26 Petunia x hybrida nam-like protein 4. 389 G2053 gi15148912 8.50E−26 Phaseolus vulgaris NAC domain protein NAC1. 389 G2053 gi19225018 1.10E−25 Oryza sativa (japonica putative NAM (no a cultivar-group) 389 G2053 gi7716952 1.10E−25 Medicago truncatula NAC1. 389 G2053 gi6175246 2.30E−25 Lycopersicon jasmonic acid 2. esculentum 389 G2053 gi4218535 2.00E−24 Triticum sp. GRAB1 protein. 389 G2053 gi6730936 2.00E−24 Oryza sativa OsNAC3 protein. 389 G2053 gi6732154 2.00E−24 Triticum monococcum unnamed protein product. 389 G2053 gi14485513 6.20E−23 Solanum tuberosum putative NAC domain protein. 391 G2059 AW257352 3.00E−44 Medicago truncatula EST305489 KV2 Medicago truncatula cDNA 391 G2059 BI972689 1.00E−36 Glycine max sai81e12.y1 Gm-c1065 Glycine max cDNA clone GEN 391 G2059 BQ408107 5.00E−29 Gossypium arboreum GA_Ed0006B09f Gossypium arboreum 7-10 d 391 G2059 BI922932 2.00E−28 Lycopersicon EST542836 tomato callus esculentum Lycopersico 391 G2059 CA018649 6.00E−28 Hordeum vulgare subsp. HV09E02r HV Hordeum vulgare vulgare 391 G2059 BM406373 6.00E−28 Solanum tuberosum EST580796 potato roots Solanum tuberosum 391 G2059 AW618459 1.00E−27 Lycopersicon pennellii EST320445 L. pennellii trichome, Cor 391 G2059 BI958427 1.00E−27 Hordeum vulgare HVSMEn0014O18f Hordeum vulgare rachis EST 1 391 G2059 BU894329 1.00E−27 Populus tremula x X007E05 Populus wood Populus tremuloides 391 G2059 AI166481 5.00E−27 Populus balsamifera xylem.est.309 Poplar subsp. trichocarpa 391 G2059 gi19920190 1.40E−29 Oryza sativa (japonica Putative AP2 domai cultivar-group) 391 G2059 gi8571476 1.90E−28 Atriplex hortensis apetala2 domain-containing protein. 391 G2059 gi21908036 4.60E−27 Zea mays DRE binding factor 1. 391 G2059 gi14140163 3.30E−23 Oryza sativa putative AP2 domain containing protein. 391 G2059 gi131754 1.60E−19 Lupinus polyphyllus PPLZ02 PROTEIN. 391 G2059 gi3342211 2.10E−19 Lycopersicon Pti4. esculentum 391 G2059 gi1208497 1.90E−18 Nicotiana tabacum EREBP-4. 391 G2059 gi20303011 5.70E−18 Brassica napus CBF-like protein CBF5. 391 G2059 gi19071243 7.40E−18 Hordeum vulgare CRT/DRE binding factor 1. 391 G2059 gi7528276 9.70E−18 Mesembryanthemum AP2-related transcription f crystallinum 393 G2085 BI498544 7.00E−59 Glycine max sai15e07.y1 Gm-c1053 Glycine max cDNA clone GEN 393 G2085 BM437375 8.00E−47 Vitis vinifera VVA018E12_54245 An expressed sequence tag da 393 G2085 BI308204 7.00E−46 Medicago truncatula EST529614 GPOD Medicago truncatula cDNA 393 G2085 BQ295376 8.00E−45 Triticum aestivum WHE2869_C08_F15ZS Wheat unstressed root t 393 G2085 BF199732 7.00E−44 Triticum monococcum WHE0591- 0594_H22_H22ZE Triticum monococ 393 G2085 AY103800 2.00E−43 Zea mays PCO084138 mRNA sequence. 393 G2085 BH723453 3.00E−40 Brassica oleracea BOMBQ10TR BO_2_3_KB Brassica oleracea gen 393 G2085 BU993000 4.00E−39 Hordeum vulgare HD12E09r HD Hordeum vulgare cDNA clone HD12 393 G2085 BU815658 4.00E−38 Populus tremula x N044F04 Populus bark Populus tremuloides 393 G2085 BQ987329 1.00E−36 Lactuca sativa QGF11O18.yg.ab1 QG_EFGHJ lettuce serriola La 393 G2085 gi13174240 3.20E−42 Oryza sativa putative zinc finger protein. 393 G2085 gi23237937 4.30E−09 Oryza sativa (japonica transposase-like. cultivar-group) 393 G2085 gi12711287 0.00061 Nicotiana tabacum GATA-1 zinc finger protein. 393 G2085 gi21655162 0.0027 Hordeum vulgare subsp. CONSTANS-like protein vulgare CO9. 393 G2085 gi1076609 0.015 Nicotiana NTL1 protein-curled- plumbaginifolia leaved to 393 G2085 gi22854920 0.017 Brassica nigra COL1 protein. 393 G2085 gi3341723 0.082 Raphanus sativus CONSTANS-like 1 protein. 393 G2085 gi21667485 0.15 Hordeum vulgare CONSTANS-like protein. 393 G2085 gi4091804 0.46 Malus x domestica CONSTANS-like protein 1. 393 G2085 gi2303681 0.49 Brassica napus unnamed protein product. 395 G2105 BM110736 3.00E−50 Solanum tuberosum EST558272 potato roots Solanum tuberosum 395 G2105 BQ866994 2.00E−49 Lactuca sativa QGC9I02.yg.ab1 QG_ABCDI lettuce salinas Lact 395 G2105 BH975294 6.00E−45 Brassica oleracea odh15d05.b1 B. oleracea002 Brassica olerac 395 G2105 BF646615 2.00E−41 Medicago truncatula NF066C08EC1F1065 Elicited cell culture 395 G2105 OSGT2 4.00E−32 Oryza sativa O. sativa gt-2 gene. 395 G2105 AI777252 1.00E−28 Lycopersicon EST258217 tomato esculentum resistant, Cornell 395 G2105 BU049946 3.00E−27 Zea mays 1111017E09.y1 1111- Unigene III from Maize Genome 395 G2105 AB052729 4.00E−26 Pisum sativum mRNA for DNA-binding protein DF1, complete cd 395 G2105 AF372499 4.00E−25 Glycine max GT-2 factor mRNA, partial cds. 395 G2105 BU889446 4.00E−24 Populus tremula P021A05 Populus petioles cDNA library Popul 395 G2105 gi13646986 2.40E−39 Pisum sativum DNA-binding protein DF1. 395 G2105 gi20249 2.20E−35 Oryza sativa gt-2. 395 G2105 gi18182311 1.70E−27 Glycine max GT-2 factor. 395 G2105 gi20161567 9.10E−08 Oryza sativa (japonica hypothetical prote cultivar-group) 395 G2105 gi170271 1.70E−05 Nicotiana tabacum DNA-binding protein. 395 G2105 gi4456620 0.36 Hordeum vulgare alpha-galactosidase. 395 G2105 gi3645898 0.68 Zea mays in-frame stop codon; possibly a post-transpositi 395 G2105 gi531098 0.95 Zinnia elegans TED3. 395 G2105 gi1657853 1 Triticum aestivum cold acclimation protein WCOR825. 395 G2105 gi20086402 1 Isoetes asiatica LFY homolog. 397 G2110 BH472587 4.00E−87 Brassica oleracea BOGPM69TR BOGP Brassica oleracea genomic 397 G2110 BI422533 9.00E−57 Lycopersicon EST533199 tomato callus, esculentum TAMU Lycop 397 G2110 AP002486 1.00E−52 Oryza sativa genomic DNA, chromosome 1, PAC clone: P0510F03. 397 G2110 AAAA01001635 2.00E−52 Oryza sativa (indica ( ) scaffold001635 cultivar-group) 397 G2110 BM370908 1.00E−47 Hordeum vulgare EBro04_SQ002_M09_R IGF Barley EBro04 librar 397 G2110 AU083645 5.00E−44 Cryptomeria japonica AU083645 Cryptomeria japonica inner ba 397 G2110 BG551253 2.00E−43 Glycine max sad35a10.y1 Gm-c1074 Glycine max cDNA clone GEN 397 G2110 BQ625082 3.00E−43 Citrus sinensis USDA-FP_02173 Ridge pineapple sweet orange 397 G2110 BF636342 5.00E−42 Medicago truncatula NF088G12DT1F1099 Drought Medicago trunc 397 G2110 BG838724 2.00E−40 Glycine clandestina Gc02_02f10_R Gc02_AAFC_ECORC_cold_stres 397 G2110 gi11320830 4.00E−59 Oryza sativa putative WRKY DNA binding protein. 397 G2110 gi20160973 4.00E−35 Oryza sativa (japonica hypothetical prote cultivar-group) 397 G2110 gi1159879 1.20E−27 Avena fatua DNA-binding protein. 397 G2110 gi11493822 1.50E−27 Petroselinum crispum transcription factor WRKY4. 397 G2110 gi6683537 1.10E−25 Nicotiana tabacum TMV response-related gene product. 397 G2110 gi4894965 1.70E−20 Avena sativa DNA-binding protein WRKY1. 397 G2110 gi18158619 7.50E−20 Retama raetam WRKY-like drought- induced protein. 397 G2110 gi24745606 2.20E−19 Solanum tuberosum WRKY-type DNA binding protein. 397 G2110 gi1076685 3.90E−19 Ipomoea batatas SPF1 protein-sweet potato. 397 G2110 gi13620227 4.30E−19 Lycopersicon hypothetical protein. esculentum 399 G2114 AX555218 2.00E−99 Glycine max Sequence 3 from Patent WO02059332. 399 G2114 AX555220 2.00E−94 Oryza sativa Sequence 5 from Patent WO02059332. 399 G2114 AF317904 3.00E−94 Brassica napus AP2/EREBP transcription factor BABY BOOM1 (B 399 G2114 AY109146 3.00E−89 Zea mays PCO137288 mRNA sequence. 399 G2114 BJ188928 9.00E−87 Physcomitrella patens BJ188928 normalized ful subsp. patens 399 G2114 BQ864461 2.00E−78 Lactuca sativa QGC26M12.yg.ab1 QG_ABCDI lettuce salinas Lac 399 G2114 BQ122372 2.00E−74 Medicago truncatula EST607948 GLSD Medicago truncatula cDNA 399 G2114 BQ625052 2.00E−70 Citrus sinensis USDA-FP_02143 Ridge pineapple sweet orange 399 G2114 AJ475492 2.00E−66 Hordeum vulgare AJ475492 S00008 Hordeum vulgare cDNA clone 399 G2114 BJ312281 5.00E−66 Triticum aestivum BJ312281 Y. Ogihara unpublished cDNA libr 399 G2114 gi21069051 2.10E−95 Brassica napus AP2/EREBP transcription factor BABY BOOM1. 399 G2114 gi21304227 7.10E−90 Oryza sativa ovule development aintegumenta-like protein 399 G2114 gi20161013 9.10E−90 Oryza sativa (japonica putative ovule dev cultivar-group) 399 G2114 gi2652938 1.20E−83 Zea mays orf. 399 G2114 gi18476518 2.10E−45 Hordeum vulgare APETALA2-like protein. 399 G2114 gi5081557 2.60E−45 Petunia x hybrida PHAP2B protein. 399 G2114 gi11181612 6.40E−44 Picea abies APETALA2-related transcription factor 2. 399 G2114 gi13173164 9.40E−43 Pisum sativum APETAL2-like protein. 399 G2114 gi21717332 4.10E−42 Malus x domestica transcription factor AHAP2. 399 G2114 gi5360996 1.10E−34 Hyacinthus orientalis APETALA2 protein homolog HAP2. 401 G2117 BH928153 3.00E−36 Brassica oleracea odi35d09.b1 B. oleracea002 Brassica olerac 401 G2117 BU080897 9.00E−21 Glycine max saq31e07.y1 Gm-c1045 Glycine max cDNA clone SOY 401 G2117 BI977302 9.00E−21 Rosa chinensis eG09 Old Blush petal SMART library Rosa chin 401 G2117 BI417596 4.00E−19 Lotus japonicus LjNEST33b4r Lotus japonicus nodule library 401 G2117 BE450859 6.00E−19 Lycopersicon EST401746 tomato root, esculentum plants pre-a 401 G2117 BE941078 6.00E−19 Medicago truncatula EST420657 MGHG Medicago truncatula cDNA 401 G2117 BM300051 8.00E−19 Mesembryanthemum MCR054F01_24630 Ice crystallinum plant Lam 401 G2117 AF350505 2.00E−17 Phaseolus vulgaris bZip transcription factor mRNA, complete 401 G2117 AY026054 8.00E−17 Phaseolus acutifolius bZIP mRNA, complete cds. 401 G2117 AAAA01000368 1.00E−14 Oryza sativa (indica ( ) scaffold000368 cultivar-group) 401 G2117 gi13430400 1.50E−19 Phaseolus vulgaris bZip transcription factor. 401 G2117 gi12829956 3.20E−19 Phaseolus acutifolius bZIP. 401 G2117 gi10241920 8.00E−14 Nicotiana tabacum TBZF. 401 G2117 gi5901747 5.60E−13 Lycopersicon bZIP DNA-binding protein. esculentum 401 G2117 gi9650826 5.60E−13 Petroselinum crispum common plant regulatory factor 6. 401 G2117 gi22597162 5.10E−12 Glycine max bZIP transcription factor ATB2. 401 G2117 gi2244742 5.10E−12 Antirrhinum majus bZIP DNA-binding protein. 401 G2117 gi13236840 2.00E−11 Catharanthus roseus G-box binding factor bZIP transcripti 401 G2117 gi435942 4.40E−11 Oryza sativa DNA-binding factor of bZIP class. 401 G2117 gi24460973 150E−10 Capsicum chinense bZIP transcription factor. 403 G2123 AX281102 2.00E−58 Physcomitrella patens Sequence 8 from Patent WO0177355. 403 G2123 BU836035 1.00E−56 Populus tremula x T081H08 Populus apica Populus tremuloides 403 G2123 AF272573 2.00E−56 Populus alba x Populus clone INRA717-1-B4 14-3- tremula 3 pr 403 G2123 BM436731 2.00E−56 Vitis vinifera VVA008H10_53045 An expressed sequence tag da 403 G2123 AB071968 4.00E−55 Nicotiana tabacum D75 mRNA for 14-3-3 protein, complete cds 403 G2123 BM411329 4.00E−55 Lycopersicon EST585656 tomato breaker esculentum fruit Lyco 403 G2123 BM408090 4.00E−55 Solanum tuberosum EST582417 potato roots Solanum tuberosum 403 G2123 BG581482 5.00E−55 Medicago truncatula EST483216 GVN Medicago truncatula cDNA 403 G2123 BQ994376 5.00E−55 Lactuca sativa QGF7A23.yg.ab1 QG_EFGHJ lettuce serriola Lac 403 G2123 AF228501 1.00E−54 Glycine max 14-3-3-like protein mRNA, complete cds. 403 G2123 gi8515890 1.10E−55 Populus alba x Populus 14-3-3 protein. tremula 403 G2123 gi8099061 2.30E−55 Populus x canescens 14-3-3 protein. 403 G2123 gi15637114 2.10E−54 Lycopersicon 14-3-3 family protein. esculentum 403 G2123 gi15778154 2.70E−54 Nicotiana tabacum 14-3-3 protein. 403 G2123 gi1575731 5.50E−54 Glycine max SGF14D. 403 G2123 gi2822483 3.90E−53 Maackia amurensis 14-3-3 protein homolog. 403 G2123 gi6752903 6.30E−53 Euphorbia esula 14-3-3-like protein. 403 G2123 gi1076543 1.30E−52 Vicia faba 14-3-3 protein homolog Vfa-1433b-fava bean. 403 G2123 gi16755676 1.30E−52 Fritillaria cirrhosa 14-3-3 protein. 403 G2123 gi15984178 3.50E−52 Nicotiana benthamiana unnamed protein product. 405 G2130 BH556628 1.00E−83 Brassica oleracea BOHAM82TF BOHA Brassica oleracea genomic 405 G2130 AP004902 4.00E−35 Lotus japonicus genomic DNA, chromosome 2, clone: LjT04G24, 405 G2130 AW685524 5.00E−34 Medicago truncatula NF031C12NR1F1000 Nodulated root Medicag 405 G2130 BM886518 2.00E−30 Glycine max sam17f08.y1 Gm-c1068 Glycine max cDNA clone SOY 405 G2130 LEU89257 1.00E−28 Lycopersicon DNA-binding protein Pti6 esculentum mRNA, comp 405 G2130 AAAA01000263 3.00E−27 Oryza sativa (indica ( ) scaffold000263 cultivar-group) 405 G2130 AB026295 3.00E−27 Oryza sativa genomic DNA, chromosome 6, clone: P0681F10, com 405 G2130 BQ873772 6.00E−27 Lactuca sativa QGI2I03.yg.ab1 QG_ABCDI lettuce salinas Lact 405 G2130 AF058827 2.00E−25 Nicotiana tabacum TSI1 (Tsi1) mRNA, complete cds. 405 G2130 BQ704534 2.00E−20 Brassica napus Bn01_03k04_A 405 G2130 gi2213785 5.50E−31 Lycopersicon Pti6. esculentum 405 G2130 gi5295944 1.70E−29 Oryza sativa Similar to Nicotiana tabacum mRNA for ERF1, 405 G2130 gi3065895 1.30E−27 Nicotiana tabacum TSI1. 405 G2130 gi8809571 6.00E−22 Nicotiana sylvestris ethylene-responsive element binding 405 G2130 gi7528276 6.00E−22 Mesembryanthemum AP2-related transcription f crystallinum 405 G2130 gi22415744 1.20E−21 Zea mays AP2 domain transcription factor. 405 G2130 gi8571476 1.40E−20 Atriplex hortensis apetala2 domain-containing protein. 405 G2130 gi24817250 3.50E−20 Cicer arietinum transcription factor EREBP- like protein. 405 G2130 gi20805105 6.30E−20 Oryza sativa (japonica contains ESTs AU06 cultivar-group) 405 G2130 gi4099921 2.50E−19 Stylosanthes hamata EREBP-3 homolog. 407 G2133 BH420519 1.00E−53 Brassica oleracea BOGUH88TF BOGU Brassica oleracea genomic 407 G2133 BG543936 6.00E−43 Brassica rapa subsp. E1686 Chinese cabbage pekinensis etiol 407 G2133 AU292603 2.00E−28 Zinnia elegans AU292603 zinnia cultured mesophyll cell equa 407 G2133 BE320193 6.00E−24 Medicago truncatula NF024B04RT1F1029 Developing root Medica 407 G2133 AP003346 3.00E−22 Oryza sativa chromosome 1 clone P0434C04, *** SEQUENCING IN 407 G2133 AAAA01000718 3.00E−22 Oryza sativa (indica ( ) scaffold000718 cultivar-group) 407 G2133 AC124836 6.00E−22 Oryza sativa (japonica ( ) chromosome 5 clo cultivar-group) 407 G2133 BZ403609 2.00E−20 Zea mays OGABN17TM ZM_0.7_1.5_KB Zea mays genomic clone ZMM 407 G2133 BM985484 6.00E−19 Thellungiella halophila 10_C12_T Ath Thellungiella halophil 407 G2133 BM403179 3.00E−17 Selaginella lepidophylla SLA012F10_35741 An expressed seque 407 G2133 gi20161239 6.90E−24 Oryza sativa (japonica hypothetical prote cultivar-group) 407 G2133 gi8571476 6.00E−17 Atriplex hortensis apetala2 domain-containing protein. 407 G2133 gi14140155 7.80E−16 Oryza sativa putative AP2 domain transcription factor. 407 G2133 gi5616086 7.00E−15 Brassica napus dehydration responsive element binding pro 407 G2133 gi21908034 8.90E−15 Zea mays DRE binding factor 2. 407 G2133 gi19071243 6.30E−14 Hordeum vulgare CRT/DRE binding factor 1. 407 G2133 gi18535580 2.10E−13 Lycopersicon putative transcriptional esculentum activato 407 G2133 gi1208496 3.30E−13 Nicotiana tabacum EREBP-3. 407 G2133 gi8980313 4.40E−13 Catharanthus roseus AP2-domain DNA-binding protein. 407 G2133 gi15488459 2.20E−12 Triticum aestivum AP2-containing protein. 409 G2138 BH545016 2.00E−63 Brassica oleracea BOHFD22TR BOHF Brassica oleracea genomic 409 G2138 BQ704534 7.00E−43 Brassica napus Bn01_03k04_A 409 G2138 AP004902 6.00E−28 Lotus japonicus genomic DNA, chromosome 2, clone: LjT04G24, 409 G2138 BM886518 1.00E−25 Glycine max sam17f08.y1 Gm-c1068 Glycine max cDNA clone SOY 409 G2138 AW685524 2.00E−25 Medicago truncatula NF031C12NR1F1000 Nodulated root Medicag 409 G2138 BQ873772 1.00E−23 Lactuca sativa QGI2I03.yg.ab1 QG_ABCDI lettuce salinas Lact 409 G2138 AF058827 9.00E−22 Nicotiana tabacum TSI1 (Tsi1) mRNA, complete cds. 409 G2138 LEU89257 2.00E−20 Lycopersicon DNA-binding protein Pti6 esculentum mRNA, comp 409 G2138 BG350434 5.00E−20 Solanum tuberosum 091E08 Mature tuber lambda ZAP Solanum tu 409 G2138 AP002835 1.00E−17 Oryza sativa genomic DNA, chromosome 1, PAC clone: P0417G05. 409 G2138 gi3065895 9.30E−20 Nicotiana tabacum TSI1. 409 G2138 gi5295944 1.50E−19 Oryza sativa Similar to Nicotiana tabacum mRNA for ERF1, 409 G2138 gi2213785 1.40E−18 Lycopersicon Pti6. esculentum 409 G2138 gi8809573 1.60E−17 Nicotiana sylvestris ethylene-responsive element binding 409 G2138 gi8571476 1.80E−16 Atriplex hortensis apetala2 domain-containing protein. 409 G2138 gi21908036 4.80E−16 Zea mays DRE binding factor 1. 409 G2138 gi3264767 7.80E−16 Prunus armeniaca AP2 domain containing protein. 409 G2138 gi23617235 1.30E−15 Oryza sativa (japonica contains ESTs AU16 cultivar-group) 409 G2138 gi4099921 7.00E−15 Stylosanthes hamata ERFBP-3 homolog. 409 G2138 gi24817250 1.50E−14 Cicer arietinum transcription factor EREBP- like protein. 411 G2140 BH501999 1.00E−70 Brassica oleracea BOHLI02TF BOHL Brassica oleracea genomic 411 G2140 AI488313 5.00E−66 Lycopersicon EST246635 tomato ovary, esculentum TAMU Lycope 411 G2140 BE020519 2.00E−60 Glycine max sm44g03.y1 Gm-c1028 Glycine max cDNA clone GENO 411 G2140 AU093196 1.00E−51 Oryza sativa subsp. AU093196 Rice callus japonica Oryza sat 411 G2140 BF647687 2.00E−41 Medicago truncatula NF025A04EC1F1024 Elicited cell culture 411 G2140 BH860622 7.00E−39 Populus balsamifera ORNL097 Poplar BAC L subsp. trichocarpa 411 G2140 BU813371 1.00E−38 Populus tremula x N009F04 Populus bark Populus tremuloides 411 G2140 AC125495 8.00E−38 Oryza sativa (japonica ( ) chromosome 3 clo cultivar-group) 411 G2140 BU891490 4.00E−35 Populus tremula P051C02 Populus petioles cDNA library Popul 411 G2140 AI054433 3.00E−34 Mesembryanthemum R6-R97 Ice plant Lambda crystallinum Uni-Z 411 G2140 gi8570062 8.90E−31 Oryza sativa ESTs C26093(C11622), AU090634 (C12429) corresp 411 G2140 gi21327944 1.80E−30 Oryza sativa (japonica contains ESTs AU06 cultivar-group) 411 G2140 gi527655 3.80E−10 Pennisetum glaucum myc-like regulatory R gene product. 411 G2140 gi527661 7.80E−09 Phyllostachys acuta myc-like regulatory R gene product. 411 G2140 gi527665 1.70E−08 Sorghum bicolor myc-like regulatory R gene product. 411 G2140 gi114217 2.60E−08 Zea mays ANTHOCYANIN REGULATORY R-S PROTEIN. 411 G2140 gi527663 9.60E−08 Tripsacum australe myc-like regulatory R gene product. 411 G2140 gi1086526 1.20E−07 Oryza australiensis transcriptional activator Ra homolog. 411 G2140 gi1086534 1.60E−07 Oryza officinalis transcriptional activator Ra homolog. 411 G2140 gi1086536 9.20E−07 Oryza rufipogon transcriptional activator Ra homolog. 413 G2143 BH650724 5.00E−76 Brassica oleracea BOMIW43TR BO_2_3_KB Brassica oleracea gen 413 G2143 CA783614 1.00E−43 Glycine max sat50g04.y1 Gm-c1056 Glycine max cDNA clone SOY 413 G2143 BE451174 9.00E−43 Lycopersicon EST402062 tomato root, esculentum plants pre-a 413 G2143 AP004693 6.00E−41 Oryza sativa chromosome 8 clone P0461F06, *** SEQUENCING IN 413 G2143 AAAA01006870 7.00E−40 Oryza sativa (indica ( ) scaffold006870 cultivar-group) 413 G2143 AP005655 7.00E−40 Oryza sativa (japonica ( ) chromosome 9 clo cultivar-group) 413 G2143 BH775806 2.00E−34 Zea mays fzmb011f018c05f1 fzmb filtered library Zea mays ge 413 G2143 AT002234 1.00E−33 Brassica rapa subsp. AT002234 Flower bud pekinensis cDNA Br 413 G2143 BF263465 3.00E−27 Hordeum vulgare HV_CEa0006N02f Hordeum vulgare seedling gre 413 G2143 CA015528 3.00E−25 Hordeum vulgare subsp. HT14J12r HT Hordeum vulgare vulgare 413 G2143 gi19571105 9.20E−29 Oryza sativa (japonica hypothetical prote cultivar-group) 413 G2143 gi15528743 1.10E−26 Oryza sativa contains EST C74560(E31855)~unknown protein. 413 G2143 gi1086538 1.60E−09 Oryza rufipogon transcriptional activator Rb homolog. 413 G2143 gi6166283 2.30E−09 Pinus taeda helix-loop-helix protein 1A. 413 G2143 gi1142621 9.70E−08 Phaseolus vulgaris phaseolin G-box binding protein PG2. 413 G2143 gi3399777 1.10E−07 Glycine max symbiotic ammonium transporter; nodulin. 413 G2143 gi5923912 1.30E−07 Tulipa gesneriana bHLH transcription factor GBOF-1. 413 G2143 gi10998404 1.90E−07 Petunia x hybrida anthocyanin 1. 413 G2143 gi4321762 1.10E−06 Zea mays transcription factor MYC7E. 413 G2143 gi166428 1.30E−06 Antirrhinum majus DEL. 415 G2144 BQ404603 4.00E−59 Gossypium arboreum GA_Ed0072F04f Gossypium arboreum 7-10 d 415 G2144 BQ517427 3.00E−53 Solanum tuberosum EST624842 Generation of a set of potato c 415 G2144 BQ583438 3.00E−51 Beta vulgaris E011979-024-005-B19-SP6 MPIZ-ADIS-024-inflore 415 G2144 BQ122428 6.00E−50 Medicago truncatula EST608004 GLSD Medicago truncatula cDNA 415 G2144 BI427219 1.00E−49 Glycine max sah77g01.y1 Gm-c1049 Glycine max cDNA clone GEN 415 G2144 AI725733 1.00E−40 Gossypium hirsutum BNLGHi12783 Six-day Cotton fiber Gossypi 415 G2144 BH999551 2.00E−38 Brassica oleracea oeg96e04.b1 B. oleracea002 Brassica olerac 415 G2144 BI926089 1.00E−33 Lycopersicon EST545978 tomato flower, esculentum buds 0-3 m 415 G2144 BU791131 4.00E−33 Populus balsamifera subsp. trichocarpa x Populus deltoides 415 G2144 BU015022 2.00E−32 Lactuca sativa QGJ9A23.yg.ab1 QG_EFGHJ lettuce serriola Lac 415 G2144 gi20804997 2.70E−36 Oryza sativa (japonica DNA-binding protei cultivar-group) 415 G2144 gi11862964 2.70E−34 Oryza sativa hypothetical protein. 415 G2144 gi5923912 8.60E−33 Tulipa gesneriana bHLH transcription factor GBOF-1. 415 G2144 gi6166283 5.10E−09 Pinus taeda helix-loop-helix protein 1A. 415 G2144 gi3399777 3.00E−05 Glycine max symbiotic ammonium transporter; nodulin. 415 G2144 gi1086538 6.70E−05 Oryza rufipogon transcriptional activator Rb homolog. 415 G2144 gi13346180 0.00013 Gossypium hirsutum GHDEL61. 415 G2144 gi527655 0.00021 Pennisetum glaucum myc-like regulatory R gene product. 415 G2144 gi527665 0.00029 Sorghum bicolor myc-like regulatory R gene product. 415 G2144 gi527661 0.00033 Phyllostachys acuta myc-like regulatory R gene product. 417 G2153 BH566718 1.00E−127 Brassica oleracea BOHCV23TR BOHC Brassica oleracea genomic 417 G2153 AP004971 2.00E−90 Lotus japonicus genomic DNA, chromosome 5, clone: LjT45G21, 417 G2153 AP004020 1.00E−79 Oryza sativa chromosome 2 clone OJ1119_A01, *** SEQUENCING 417 G2153 AAAA01017331 2.00E−72 Oryza sativa (indica ( ) scaffold017331 cultivar-group) 417 G2153 BQ165495 2.00E−67 Medicago truncatula EST611364 KVKC Medicago truncatula cDNA 417 G2153 AP005653 1.00E−66 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 417 G2153 BQ785950 8.00E−64 Glycine max saq61f09.y1 Gm-c1076 Glycine max cDNA clone SOY 417 G2153 BZ412041 3.00E−63 Zea mays OGACG56TC ZM_0.7_1.5_KB Zea mays genomic clone ZMM 417 G2153 BM110212 3.00E−63 Solanum tuberosum EST557748 potato roots Solanum tuberosum 417 G2153 BQ865858 7.00E−63 Lactuca sativa QGC6B08.yg.ab1 QG_ABCDI lettuce salinas Lact 417 G2153 gi24059979 3.80E−39 Oryza sativa (japonica similar to DNA-bin cultivar-group) 417 G2153 gi15528814 1.70E−36 Oryza sativa hypothetical protein~similar to Arabidopsis 417 G2153 gi4165183 5.00E−21 Antirrhinum majus SAP1 protein. 417 G2153 gi2213534 1.30E−19 Pisum sativum DNA-binding PD1-dike protein. 417 G2153 gi7439981 2.60E−08 Triticum aestivum glycine-rich RNA-binding protein GRP1- 417 G2153 gi21623 1.90E−06 Sorghum bicolor glycine-rich RNA-binding protein. 417 G2153 gi11545668 3.50E−06 Chlamydomonas CIA5. reinhardtii 417 G2153 gi21068672 6.60E−06 Cicer arietinum putative glicine-rich protein. 417 G2153 gi7489714 6.60E−06 Zea mays aluminum-induced protein al1-maize. 417 G2153 gi395147 1.60E−05 Nicotiana tabacum glycine-rich protein. 419 G2155 BG543096 2.00E−69 Brassica rapa subsp. E0571 Chinese cabbage pekinensis etiol 419 G2155 BH480897 7.00E−66 Brassica oleracea BOGRA01TF BOGR Brassica oleracea genomic 419 G2155 BG646893 2.00E−53 Medicago truncatula EST508512 HOGA Medicago truncatula cDNA 419 G2155 BU023570 3.00E−44 Helianthus annuus QHF11M19.yg.ab1 QH_EFGHJ sunflower RHA280 419 G2155 AP004020 2.00E−41 Oryza sativa chromosome 2 clone OJ1119_A01, *** SEQUENCING 419 G2155 BI426899 4.00E−41 Glycine max sag08g12.y1 Gm-c1080 Glycine max cDNA clone GEN 419 G2155 AAAA01000383 2.00E−40 Oryza sativa (indica ( ) scaffold000383 cultivar-group) 419 G2155 AP004971 2.00E−40 Lotus japonicus genomic DNA, chromosome 5, clone: LjT45G21, 419 G2155 AP005755 2.00E−40 Oryza sativa (japonica ( ) chromosome 9 clo cultivar-group) 419 G2155 BZ412041 8.00E−39 Zea mays OGACG56TC ZM_0.7_1.5_KB Zea mays genomic clone ZMM 419 G2155 gi15528814 3.70E−32 Oryza sativa hypothetical protein~similar to Arabidopsis 419 G2155 gi24059979 1.20E−21 Oryza sativa (japonica similar to DNA-bin cultivar-group) 419 G2155 gi4165183 3.50E−20 Antirrhinum majus SAP1 protein. 419 G2155 gi2213534 1.60E−16 Pisum sativum DNA-binding PD1-like protein. 419 G2155 gi2224911 0.98 Daucus carota somatic embryogenesis receptor-like kinase. 419 G2155 gi454279 1 Avena sativa DNA-binding protein. 421 G2192 AY061812  1.0e−999 Brassica nigra Lm1 mRNA, complete sequence. 421 G2192 BH544406 1.00E−118 Brassica oleracea BOGYW04TF BOGY Brassica oleracea genomic 421 G2192 AC131240 1.00E−98 Medicago truncatula clone mth2-33j22, WORKING DRAFT SEQUENC 421 G2192 LJA239041 3.00E−92 Lotus japonicus mRNA for nodule inception protein (nin). 421 G2192 AP001539 2.00E−90 Oryza sativa genomic DNA, chromosome 1, clone: P0708G02. 421 G2192 AAAA01000250 2.00E−90 Oryza sativa (indica ( ) scaffold000250 cultivar-group) 421 G2192 BU007504 2.00E−85 Lactuca sativa QGH3e07.yg.ab1 QG_EFGHJ lettuce serriola Lac 421 G2192 BF272061 2.00E−71 Gossypium arboreum GA_Eb0013L09f Gossypium arboreum 7-10 d 421 G2192 BE600221 1.00E−69 Sorghum bicolor PI1_80_G08.b1_A002 Pathogen induced 1 (PI1) 421 G2192 BG508620 2.00E−66 Glycine max sac75c04.y1 Gm-c1072 Glycine max cDNA clone GEN 421 G2192 gi7339715 2.20E−187 Oryza sativa EST AU057816(S21817) corresponds to a region 421 G2192 gi20503001 2.40E−132 Oryza sativa (japonica Putataive nodule i cultivar-group) 421 G2192 gi6448579 3.20E−95 Lotus japonicus nodule inception protein. 421 G2192 gi23504757 8.10E−95 Pisum sativum nodule inception protein. 421 G2192 gi2190980 0.0002 Chlamydomonas incerta minus dominance gene product. 421 G2192 gi1928929 0.0021 Chlamydomonas minus dominance protein. reinhardtii 421 G2192 gi100897 0.48 Zea mays Lc regulatory protein- maize. 421 G2192 gi170732 0.93 Triticum aestivum gamma-gliadin. 421 G2192 gi13346180 0.97 Gossypium hirsutum GHDEL61. 421 G2192 gi100212 1 Lycopersicon extensin class II (clones esculentum u1/u2) 423 G2295 BZ059285 1.00E−27 Brassica oleracea llf45f10.b1 B. oleracea002 Brassica olerac 423 G2295 AAAA01000422 7.00E−13 Oryza sativa (indica ( ) scaffold000422 cultivar-group) 423 G2295 AP002480 7.00E−13 Oryza sativa genomic DNA, chromosome 1, clone: P0469E05. 423 G2295 AW508033 9.00E−11 Glycine max si49c04.y1 Gm-r1030 Glycine max cDNA clone GENO 423 G2295 AC135316 3.00E−09 Medicago truncatula clone mth2-2018, WORKING DRAFT SEQUENCE 423 G2295 BE054256 3.00E−07 Gossypium arboreum GA_Ea0026J19f Gossypium arboreum 7-10 d 423 G2295 BH023181 8.00E−07 Gossypium hirsutum GH_MBb0004F02r Gossypium hirsutum L. Gos 423 G2295 BZ344426 2.00E−06 Sorghum bicolor hp63g11.b1 WGS-SbicolorF (JM107 adapted met 423 G2295 AX540653 9.00E−06 Zea mays Sequence 9 from Patent WO0240688. 423 G2295 BQ583447 1.00E−05 Beta vulgaris E011979-024-005-D15-SP6 MPIZ-ADIS-024-inflore 423 G2295 gi8096379 6.20E−15 Oryza sativa Similar to Arabidopsis thaliana chromosome 5 423 G2295 gi15623935 1.40E−09 Oryza sativa (japonica hypothetical prote cultivar-group) 423 G2295 gi3170502 9.00E−07 Papaver nudicaule APETALA3 homolog PnAP3-2. 423 G2295 gi6580943 5.40E−06 Picea abies MADS-box transcription factor. 423 G2295 gi6970411 8.30E−06 Rosa rugosa MADS-box protein. 423 G2295 gi1049022 8.40E−06 Sinapis alba transcription factor SaMADS A. 423 G2295 gi3170512 8.90E−06 Peperomia hirta APETALA3 homolog PhAP3. 423 G2295 gi23304676 1.00E−05 Brassica oleracea var. MAPS-box protein FUL-c. botrytis 423 G2295 gi4322475 1.30E−05 Eucalyptus globulus putative MADS box tra subsp. globulus 423 G2295 gi3913005 1.30E−05 Panax ginseng AGAMOUS PROTEIN (GAG2). 425 G2340 BU882839 2.00E−53 Populus balsamifera UM82TH11 Populus flo subsp. trichocarpa 425 G2340 BE054276 3.00E−53 Gossypium arboreum GA_Ea0002O18f Gossypium arboreum 7-10 d 425 G2340 PHMYBPH31 6.00E−53 Petunia x hybrida P. hybrida myb.Ph3 gene encoding protein 425 G2340 BG269414 2.00E−52 Mesembryanthemum L0-3478T3 Ice plant crystallinum Lambda Un 425 G2340 BU892831 2.00E−52 Populus tremula P070A09 Populus petioles cDNA library Popul 425 G2340 CA516461 2.00E−52 Capsicum annuum KS09058G09 KS09 Capsicum annuum cDNA, mRNA 425 G2340 OSMYB1355 4.00E−52 Oryza sativa O. sativa mRNA for myb factor, 1355 bp. 425 G2340 BG592600 9.00E−52 Solanum tuberosum EST491278 cSTS Solanum tuberosum cDNA clo 425 G2340 BG128147 1.00E−51 Lycopersicon EST473793 tomato esculentum shoot/meristem Lyc 425 G2340 BI542536 2.00E−51 Zea mays 949021A03.y1 949- Juvenile leaf and shoot cDNA fr 425 G2340 gi21739235 6.30E−53 Oryza sativa OSJNBa0072F16.14. 425 G2340 gi20563 1.70E−52 Petunia x hybrida protein 1. 425 G2340 gi13346188 5.70E−52 Gossypium hirsutum GHMYB25. 425 G2340 gi485867 8.30E−51 Antirrhinum majus mixta. 425 G2340 gi22795039 3.60E−50 Populus x canescens putative MYB transcription factor. 425 G2340 gi19072748 6.70E−49 Zea mays typical P-type R2R3 Myb protein. 425 G2340 gi22266675 4.70E−48 Vitis labrusca x Vitis myb-related transcription vinifera 425 G2340 gi19386839 9.90E−48 Oryza sativa (japonica putative myb-relat cultivar-group) 425 G2340 gi23476313 9.90E−48 Gossypium raimondii myb-like transcription factor 6. 425 G2340 gi6552389 1.20E−47 Nicotiana tabacum myb-related transcription factor LBM4. 427 G2343 LETHM1 1.00E−73 Lycopersicon L. esculentum mRNA for esculentum THM1 protein. 427 G2343 BE611938 1.00E−67 Glycine max sr01h04.y1 Gm-c1049 Glycine max cDNA clone GENO 427 G2343 BH966627 9.00E−64 Brassica oleracea odd90f02.g1 B. oleracea002 Brassica olerac 427 G2343 AV421932 1.00E−61 Lotus japonicus AV421932 Lotus japonicus young plants (two- 427 G2343 BF484214 1.00E−54 Triticum aestivum WHE2309_F07_K13ZS Wheat pre-anthesis spik 427 G2343 BU998112 5.00E−54 Hordeum vulgare subsp. HI10A14r HI Hordeum vulgare vulgare 427 G2343 AW672062 6.00E−52 Sorghum bicolor LG1_354_G05.b1_A002 Light Grown 1 (LG1) Sor 427 G2343 BI311137 6.00E−52 Medicago truncatula EST5312887 GESD Medicago truncatula cDN 427 G2343 BQ634727 4.00E−51 Pinus taeda NXRV072_E09_F NXRV (Nsf Xylem Root wood Vertica 427 G2343 AY108777 2.00E−50 Zea mays PCO139596 mRNA sequence. 427 G2343 gi1167486 1.10E−66 Lycopersicon transcription factor. esculentum 427 G2343 gi13366181 1.90E−53 Oryza sativa putative transcription factor. 427 G2343 gi22093748 2.20E−50 Oryza sativa (japonica putative myb-relat cultivar-group) 427 G2343 gi13346188 7.60E−46 Gossypium hirsutum GHMYB25. 427 G2343 gi22795039 7.60E−46 Populus x canescens putative MYB transcription factor. 427 G2343 gi20563 8.60E−45 Petunia x hybrida protein 1. 427 G2343 gi19059 1.50E−44 Hordeum vulgare MybHv33. 427 G2343 gi4886264 1.70E−43 Antirrhinum majus Myb-related transcription factor mixta- 427 G2343 gi23476313 2.80E−43 Gossypium raimondii myb-like transcription factor 6. 427 G2343 gi1732247 1.20E−42 Nicotiana tabacum transcription factor Myb1. 429 G2346 BQ403570 8.00E−43 Gossypium arboreum GA_Ed0059F05f Gossypium arboreum 7-10 d 429 G2346 AMA011622 8.00E−41 Antirrhinum majus mRNA for squamosa promoter binding 429 G2346 BQ594361 1.00E−39 Beta vulgaris S015246-024-024-K10-SP6 MPIZ-ADIS-024-develop 429 G2346 BZ040748 4.00E−39 Brassica oleracea lka41a03.g1 B. oleracea002 Brassica olerac 429 G2346 AW691786 3.00E−35 Medicago truncatula NF044B06ST1F1000 Developing stem Medica 429 G2346 BQ874863 1.00E−32 Lactuca sativa QGI6H22.yg.ab1 QG_ABCDI lettuce salinas Lact 429 G2346 ZMA011618 7.00E−29 Zea mays mRNA for SBP-domain protein 5, partial. 429 G2346 BJ245444 3.00E−27 Triticum aestivum BJ245444 Y. Ogihara unpublished cDNA libr 429 G2346 BE596165 3.00E−27 Sorghum bicolor PI1_50_D04.b1_A002 Pathogen induced 1 (PI1) 429 G2346 BG593787 4.00E−27 Solanum tuberosum EST492465 cSTS Solanum tuberosum cDNA clo 429 G2346 gi5931641 1.40E−41 Antirrhinum majus squamosa promoter binding protein-homol 429 G2346 gi5931786 1.70E−34 Zea mays SBP-domain protein 5. 429 G2346 gi8468036 7.60E−23 Oryza sativa Similar to Arabidopsis thaliana chromosome 2 429 G2346 gi9087308 3.90E−09 Mitochondrion Beta orf102a. vulgaris var. altissima 429 G2346 gi17425188 0.34 Triticum aestivum low-molecular-weight glutenin subunit g 429 G2346 gi123462 0.96 Hordeum vulgare C-HORDEIN (CLONE PC- 919). 429 G2346 gi225589 0.96 Hordeum vulgare var. hordein C. distichum 429 G2346 gi18844948 0.99 Oryza sativa (japonica hypothetical prote cultivar-group) 431 G2347 BH969114 2.00E−53 Brassica oleracea odg08d11.b1 B. oleracea002 Brassica olerac 431 G2347 BI931517 6.00E−33 Lycopersicon EST551406 tomato flower, esculentum 8 mm to pr 431 G2347 BQ989469 2.00E−32 Lactuca sativa QGF17M03.yg.ab1 QG_EFGHJ lettuce serriola La 431 G2347 CA516258 3.00E−31 Capsicum annuum KS09055D03 KS09 Capsicum annuum cDNA, mRNA 431 G2347 BE058432 5.00E−31 Glycine max sn16a06.y1 Gm-c1016 Glycine max cDNA clone GENO 431 G2347 AMSPB1 7.00E−31 Antirrhinum majus A. majus mRNA for squamosa-promoter bindin 431 G2347 BI071295 1.00E−30 Populus tremula x C054P79U Populus stra Populus tremuloides 431 G2347 BG525285 8.00E−30 Stevia rebaudiana 48-3 Stevia field grown leaf cDNA Stevia 431 G2347 BU824105 8.00E−30 Populus tremula UB60BPD08 Populus tremula cambium cDNA libr 431 G2347 L38193 9.00E−30 Brassica rapa BNAF1025E Mustard flower buds Brassica rapa c 431 G2347 gi1183864 5.40E−32 Antirrhinum majus squamosa-promoter binding protein 2. 431 G2347 gi5931786 4.60E−27 Zea mays SBP-domain protein 5. 431 G2347 gi8468036 6.90E−25 Oryza sativa Similar to Arabidopsis thaliana chromosome 2 431 G2347 gi9087308 1.40E−09 Mitochondrion Beta orf102a. vulgaris var. altissima 431 G2347 gi24414128 0.47 Oryza sativa (japonica hypothetical prote cultivar-group) 431 G2347 gi13926087 0.99 Pinus taeda alpha-tubulin. 433 G2379 BH573917 7.00E−48 Brassica oleracea BOGNX03TF BOGN Brassica oleracea genomic 433 G2379 AB072391 4.00E−45 Nicotiana tabacum NtSIP1 mRNA for 6b- interacting protein 1, 433 G2379 BG544981 7.00E−43 Brassica rapa subsp. E3094 Chinese cabbage pekinensis etiol 433 G2379 BU573650 1.00E−41 Prunus dulcis PA_Ea0004L16f Almond developing seed Prunus 433 G2379 CA801229 3.00E−40 Glycine max sau02g07.y2 Gm-c1062 Glycine max cDNA clone SOY 433 G2379 BI925592 4.00E−39 Lycopersicon EST545481 tomato flower, esculentum buds 0-3 m 433 G2379 AC113333 5.00E−39 Oryza sativa (japonica ( ) chromosome 5 clo cultivar-group) 433 G2379 AAAA01003484 8.00E−39 Oryza sativa (indica ( ) scaffold003484 cultivar-group) 433 G2379 AP003264 5.00E−38 Oryza sativa chromosome 1 clone P0485G01, *** SEQUENCING IN 433 G2379 BQ590717 3.00E−33 Beta vulgaris E012597-024-018-G24-SP6 MPIZ-ADIS-024-storage 433 G2379 gi18149189 4.80E−50 Nicotiana tabacum 6b-interacting protein 1. 433 G2379 gi21644624 2.50E−43 Oryza sativa (japonica putative 6b-intera cultivar-group) 433 G2379 gi12597883 2.30E−21 Oryza sativa hypothetical protein. 433 G2379 gi6741989 0.5 Zea mays unnamed protein product. 433 G2379 gi12231300 0.77 Lycopersicon ripening regulated protein esculentum DDTFR1 433 G2379 gi2253092 0.79 Spinacia oleracea hypothetical protein. 433 G2379 gi3288113 0.84 Beta vulgaris elongation factor 1-beta. 433 G2379 gi18419641 0.94 Narcissus putative cysteine proteinase. pseudonarcissus 433 G2379 gi1052956 0.99 Ipomoea nil high mobility group protein 2 HMG2. 433 G2379 gi14579399 1 Glycine max unknown. 435 G2430 BE214029 2.00E−23 Hordeum vulgare HV_CEb0001P06f Hordeum vulgare seedling gre 435 G2430 BQ858556 8.00E−23 Lactuca sativa QGC10J07.yg.ab1 QG_ABCDI lettuce salinas Lac 435 G2430 AU289837 1.00E−22 Zinnia elegans AU289837 zinnia cultured mesophyll cell equa 435 G2430 BM326218 1.00E−22 Sorghum bicolor PIC1_72_C05.b1_A002 Pathogen-infected compa 435 G2430 AB060130 1.00E−22 Zea mays ZmRR8 mRNA for response regulator 8, complete cds. 435 G2430 BG129795 3.00E−21 Lycopersicon EST475441 tomato esculentum shoot/meristem Lyc 435 G2430 D41804 8.00E−21 Oryza sativa RICS4626A Rice shoot Oryza sativa cDNA, mRNAs 435 G2430 BQ138699 8.00E−21 Medicago truncatula NF006C02PH1F1017 Phoma-infected Medicag 435 G2430 BU760906 3.00E−19 Glycine max sas60c07.y1 Gm-c1023 Glycine max cDNA clone SOY 435 G2430 BM407041 1.00E−18 Solanum tuberosum EST581368 potato roots Solanum tuberosum 435 G2430 gi14189890 4.70E−34 Zea mays response regulator 9. 435 G2430 gi24308616 3.00E−32 Oryza sativa (japonica Putative response cultivar-group) 435 G2430 gi6942190 3.40E−09 Mesembryanthemum CDPK substrate protein 1; C crystallinum 435 G2430 gi15289981 6.50E−09 Oryza sativa hypothetical protein. 435 G2430 gi4519671 2.30E−08 Nicotiana tabacum transfactor. 435 G2430 gi5916207 8.60E−07 Chlamydomonas regulatory protein of P- reinhardtii starvat 435 G2430 gi13173408 2.00E−05 Dianthus caryophyllus response regulator protein. 435 G2430 gi15131529 0.0024 Fragaria x ananassa ethylene receptor. 435 G2430 gi22095684 0.0051 Cucumis sativus Ethylene receptor (CS- ETR1). 435 G2430 gi11357140 0.0065 Cucumis melo var. probable ethylene receptor reticulatus 437 G2505 BU879250 5.00E−72 Populus balsamifera V057G12 Populus flow subsp. trichocarpa 437 G2505 BF645892 4.00E−70 Medicago truncatula NF042G10EC1F1083 Elicited cell culture 437 G2505 AB028186 4.00E−66 Oryza sativa mRNA for OsNAC7 protein, complete cds. 437 G2505 BF098091 4.00E−62 Lycopersicon EST428612 tomato nutrient esculentum deficient 437 G2505 BQ483881 5.00E−62 Triticum aestivum WHE3513_F08_K15ZS Wheat unstressed root c 437 G2505 BE060921 3.00E−61 Hordeum vulgare HVSMEg0013N15f Hordeum vulgare pre- anthesis 437 G2505 AAAA01001925 9.00E−57 Oryza sativa (indica ( ) scaffold001925 cultivar-group) 437 G2505 AI161918 1.00E−56 Populus tremula x A009P50U Hybrid aspen Populus tremuloides 437 G2505 CA526032 6.00E−54 Capsicum annuum KS12064G06 KS12 Capsicum annuum cDNA, mRNA 437 G2505 AP005450 2.00E−53 Oryza sativa (japonica ( ) chromosome 6 clo cultivar-group) 437 G2505 gi11875152 1.40E−66 Oryza sativa putative NAM (no apical meristem) protein. 437 G2505 gi20330750 4.30E−63 Oryza sativa (japonica Putative NAM-like cultivar-group) 437 G2505 gi1279640 4.70E−48 Petunia x hybrida NAM. 437 G2505 gi22597158 6.10E−48 Glycine max no apical meristem-like protein. 437 G2505 gi15148914 4.90E−46 Phaseolus vulgaris NAC domain protein NAC2. 437 G2505 gi4218537 4.40E−45 Triticum sp. GRAB2 protein. 437 G2505 gi6732156 4.40E−45 Triticum monococcum unnamed protein product. 437 G2505 gi6175246 1.00E−43 Lycopersicon jasmonic acid 2. esculentum 437 G2505 gi14485513 1.80E−41 Solanum tuberosum putative NAC domain protein. 437 G2505 gi7716952 6.20E−39 Medicago truncatula NAC1. 439 G2509 BH989379 8.00E−66 Brassica oleracea oed22b05.b1 B. oleracea002 Brassica olerac 439 G2509 BQ138607 4.00E−41 Medicago truncatula NF005C01PH1F1004 Phoma-infected Medicag 439 G2509 BQ786702 4.00E−36 Glycine max saq72b07.y1 Gm-c1076 Glycine max cDNA clone SOY 439 G2509 OSJN00240 7.00E−31 Oryza sativa genomic DNA, chromosome 4, BAC clone: OSJNBa0 439 G2509 AAAA01000832 7.00E−31 Oryza sativa (indica ( ) scaffold000832 cultivar-group) 439 G2509 BE419451 2.00E−29 Triticum aestivum WWS012.C2R000101 ITEC WWS Wheat Scutellum 439 G2509 BM062508 5.00E−29 Capsicum annuum KS01043F09 KS01 Capsicum annuum cDNA, mRNA 439 G2509 AI771755 2.00E−28 Lycopersicon EST252855 tomato ovary, esculentum TAMU Lycope 439 G2509 CA015575 7.00E−28 Hordeum vulgare subsp. HT14L19r HT Hordeum vulgare vulgare 439 G2509 BE642320 2.00E−27 Ceratopteris richardii Cri2_5_L17_SP6 Ceratopteris Spore Li 439 G2509 gi20160854 2.10E−29 Oryza sativa (japonica hypothetical prote cultivar-group) 439 G2509 gi3264767 8.40E−28 Prunus armeniaca AP2 domain containing protein. 439 G2509 gi24817250 1.10E−25 Cicer arietinum transcription factor EREBP- like protein. 439 G2509 gi15217291 7.10E−25 Oryza sativa Putative AP2 domain containing protein. 439 G2509 gi1208498 1.60E−24 Nicotiana tabacum EREBP-2. 439 G2509 gi8809571 1.60E−24 Nicotiana sylvestris ethylene-responsive element binding 439 G2509 gi7528276 3.00E−24 Mesembryanthemum AP2-related transcription f crystallinum 439 G2509 gi1688233 1.10E−23 Solanum tuberosum DNA binding protein homolog. 439 G2509 gi4099921 1.60E−23 Stylosanthes hamata EREBP-3 homolog. 439 G2509 gi18496063 2.40E−23 Fagus sylvatica ethylene responsive element binding prote 441 G2517 CA784851 2.00E−41 Glycine max sat90g04.y1 Gm-c1062 Glycine max cDNA clone SOY 441 G2517 BQ799236 3.00E−39 Vitis vinifera EST 1405 Green Grape berries Lambda Zap II L 441 G2517 BU884581 2.00E−36 Populus tremula x R012F08 Populus root Populus tremuloides 441 G2517 BH479877 5.00E−33 Brassica oleracea BOHNX73TR BOHN Brassica oleracea genomic 441 G2517 AW034229 2.00E−32 Lycopersicon EST277800 tomato callus, esculentum TAMU Lycop 441 G2517 AV408330 1.00E−31 Lotus japonicus AV408330 Lotus japonicus young plants (two- 441 G2517 BG889690 2.00E−31 Solanum tuberosum EST515541 cSTD Solanum tuberosum cDNA clo 441 G2517 BF645445 6.00E−30 Medicago truncatula NF040F10EC1F1090 Elicited cell culture 441 G2517 BE445081 6.00E−30 Triticum aestivum WHE1131_B06_D11ZS Wheat etiolated seedlin 441 G2517 BE362650 5.00E−28 Sorghum bicolor DG1_88_H02.b1_A002 Dark Grown 1 (DG1) Sorgh 441 G2517 gi11761085 1.00E−36 Oryza sativa putative DNA-binding protein homolog. 441 G2517 gi22830985 7.00E−31 Oryza sativa (japonica WRKY transcription cultivar-group) 441 G2517 gi4760692 9.80E−25 Nicotiana tabacum transcription factor NtWRKY2. 441 G2517 gi18158619 1.50E−23 Retama raetam WRKY-like drought- induced protein. 441 G2517 gi13620227 2.20E−23 Lycopersicon hypothetical protein. esculentum 441 G2517 gi24745606 3.80E−23 Solanum tuberosum WRKY-type DNA binding protein. 441 G2517 gi7484759 1.40E−22 Cucumis sativus SP8 binding protein homolog-cucumber. 441 G2517 gi1159877 1.60E−22 Avena fatua DNA-binding protein. 441 G2517 gi1076685 6.00E−22 Ipomoea batatas SPF1 protein-sweet potato. 441 G2517 gi11493822 1.50E−21 Petroselinum crispum transcription factor WRKY4. 443 G2520 AW928317 2.00E−48 Lycopersicon EST307050 tomato flower esculentum buds 8 mm t 443 G2520 BI270049 3.00E−47 Medicago truncatula NF004D04FL1F1042 Developing flower Medi 443 G2520 BU832739 8.00E−46 Populus tremula x T037F09 Populus apica Populus tremuloides 443 G2520 BU009829 2.00E−45 Lactuca sativa QGJ11L06.yg.ab1 QG_EFGHJ lettuce serriola La 443 G2520 BF271147 6.00E−43 Gossypium arboreum GA_Eb0010K15f Gossypium arboreum 7-10 d 443 G2520 BG725974 4.00E−42 Glycine max sae11d10.y1 Gm-c1067 Glycine max cDNA clone GEN 443 G2520 BQ509930 2.00E−41 Solanum tuberosum EST617345 Generation of a set of potato c 443 G2520 CA522636 6.00E−41 Capsicum annuum KS12008F12 KS12 Capsicum annuum cDNA, mRNA 443 G2520 BH248832 5.00E−40 Brassica oleracea BOGAN13TR BOGA Brassica oleracea genomic 443 G2520 BQ105890 1.00E−39 Rosa hybrid cultivar fc1141.e Rose Petals (Fragrant Cloud) 443 G2520 gi20804997 5.10E−35 Oryza sativa (japonica DNA-binding protei cultivar-group) 443 G2520 gi11862964 2.10E−34 Oryza sativa hypothetical protein. 443 G2520 gi5923912 6.10E−32 Tulipa gesneriana bHLH transcription factor GBOF-1. 443 G2520 gi6166283 3.30E−10 Pinus taeda helix-loop-helix protein 1A. 443 G2520 gi527655 1.10E−07 Pennisetum glaucum myc-like regulatory R gene product. 443 G2520 gi527665 4.00E−07 Sorghum bicolor myc-like regulatory R gene product. 443 G2520 gi527661 1.10E−06 Phyllostachys acuta myc-like regulatory R gene product. 443 G2520 gi13346180 1.90E−06 Gossypium hirsutum GHDEL61. 443 G2520 gi3399777 2.60E−06 Glycine max symbiotic ammonium transporter; nodulin. 443 G2520 gi1086534 4.90E−06 Oryza officinalis transcriptional activator Ra homolog. 445 G2555 BF096555 4.00E−42 Lycopersicon EST360582 tomato nutrient esculentum deficient 445 G2555 BH509718 2.00E−40 Brassica oleracea BOHGV18TF BOHG Brassica oleracea genomic 445 G2555 BF005956 3.00E−40 Medicago truncatula EST434454 DSLC Medicago truncatula cDNA 445 G2555 BU091550 3.00E−35 Glycine max st74e07.y1 Gm-c1054 Glycine max cDNA clone GENO 445 G2555 AF465824 1.00E−30 Oryza sativa transcription factor RAU1 (rau1) mRNA, partial 445 G2555 BU499331 2.00E−30 Zea mays 946174A05.y1 946-tassel primordium prepared by S 445 G2555 BU866761 6.00E−30 Populus tremula x S070E02 Populus imbib Populus tremuloides 445 G2555 CA014136 2.00E−29 Hordeum vulgare subsp. HT10H19r HT Hordeum vulgare vulgare 445 G2555 BM063750 5.00E−29 Capsicum annuum KS01059B06 KS01 Capsicum annuum cDNA, mRNA 445 G2555 AW160239 6.00E−29 Lycopersicon pennellii EST290097 L. pennellii trichome, Cor 445 G2555 gi6166283 1.70E−40 Pinus taeda helix-loop-helix protein 1A. 445 G2555 gi19401700 1.70E−34 Oryza sativa transcription factor RAU1. 445 G2555 gi20161021 2.40E−33 Oryza sativa (japonica contains ESTs AU05 cultivar-group) 445 G2555 gi5923912 1.70E−11 Tulipa gesneriana bHLH transcription factor GBOF-1. 445 G2555 gi1086538 4.50E−06 Oryza rufipogon transcriptional activator Rb homolog. 445 G2555 gi3399777 3.90E−05 Glycine max symbiotic ammonium transporter; nodulin. 445 G2555 gi527657 6.20E−05 Pennisetum glaucum myc-like regulatory R gene product. 445 G2555 gi1142619 0.00059 Phaseolus vulgaris phaseolin G-box binding protein PG1. 445 G2555 gi4206118 0.00091 Mesembryanthemum transporter homolog. crystallinum 445 G2555 gi13346182 0.0027 Gossypium hirsutum GHDEL65. 447 G2557 BH511840 1.00E−66 Brassica oleracea BOGRJ19TR BOGR Brassica oleracea genomic 447 G2557 CA799720 5.00E−49 Glycine max sat61g07.y1 Gm-c1056 Glycine max cDNA clone SOY 447 G2557 AP003296 1.00E−35 Oryza sativa chromosome 1 clone P0697C12, *** SEQUENCING IN 447 G2557 AAAA01007476 1.00E−33 Oryza sativa (indica ( ) scaffold007476 cultivar-group) 447 G2557 BF263465 1.00E−32 Hordeum vulgare HV_CEa0006N02f Hordeum vulgare seedling gre 447 G2557 AT002234 3.00E−28 Brassica rapa subsp. AT002234 Flower bud pekinensis cDNA Br 447 G2557 AP006057 1.00E−27 Oryza sativa (japonica ( ) chromosome 9 clo cultivar-group) 447 G2557 CA015528 1.00E−27 Hordeum vulgare subsp. HT14J12r HT Hordeum vulgare vulgare 447 G2557 BG557011 2.00E−27 Sorghum bicolor EM1_41_E02.g1_A002 Embryo 1 (EM1) Sorghum b 447 G2557 BH775806 7.00E−27 Zea mays fzmb011f018c05f1 fzmb filtered library Zea mays ge 447 G2557 gi15289790 5.00E−37 Oryza sativa contains EST C74560(E31855)~unknown protein. 447 G2557 gi19571105 8.40E−35 Oryza sativa (japonica hypothetical prote cultivar-group) 447 G2557 gi3399777 4.60E−07 Glycine max symbiotic ammonium transporter; nodulin. 447 G2557 gi4206118 2.10E−06 Mesembryanthemum transporter homolog. crystallinum 447 G2557 gi6166283 3.10E−06 Pinus taeda helix-loop-helix protein 1A. 447 G2557 gi5923912 6.80E−06 Tulipa gesneriana bHLH transcription factor GBOF-1. 447 G2557 gi527655 6.90E−06 Pennisetum glaucum myc-like regulatory R gene product. 447 G2557 gi527661 1.50E−05 Phyllostachys acuta myc-like regulatory R gene product. 447 G2557 gi527665 1.80E−05 Sorghum bicolor myc-like regulatory R gene product. 447 G2557 gi1086538 1.90E−05 Oryza rufipogon transcriptional activator Rb homolog. 449 G2583 BH658452 1.00E−59 Brassica oleracea BOMCP74TF BO_2_3_KB Brassica oleracea gen 449 G2583 BE023297 5.00E−54 Glycine max sm80e10.y1 Gm-c1015 Glycine max cDNA clone GENO 449 G2583 CA486875 1.00E−50 Triticum aestivum WHE4337_A02_A03ZS Wheat meiotic anther cD 449 G2583 BG642554 8.00E−48 Lycopersicon EST356031 tomato flower esculentum buds, anthe 449 G2583 BI978981 2.00E−47 Rosa chinensis zD09 Old Blush petal SMART library Rosa chin 449 G2583 BU978490 4.00E−47 Hordeum vulgare subsp. HA13G05r HA Hordeum vulgare vulgare 449 G2583 BQ106328 4.00E−46 Rosa hybrid cultivar gg1388.e Rose Petals (Golden Gate) Lam 449 G2583 BI958226 1.00E−44 Hordeum vulgare HVSMEn0013P17f Hordeum vulgare rachis EST 1 449 G2583 AP004869 1.00E−43 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 449 G2583 BU832200 6.00E−43 Populus tremula x T030G01 Populus apica Populus tremuloides 449 G2583 gi18650662 2.30E−23 Lycopersicon ethylene response factor 1. esculentum 449 G2583 gi131754 7.30E−20 Lupinus polyphyllus PPLZ02 PROTEIN. 449 G2583 gi20160854 2.80E−18 Oryza sativa (japonica hypothetical prote cultivar-group) 449 G2583 gi10798644 2.80E−18 Nicotiana tabacum AP2 domain-containing transcription fac 449 G2583 gi8571476 2.80E−18 Atriplex hortensis apetala2 domain-containing protein. 449 G2583 gi14018047 3.30E−17 Oryza sativa Putative protein containing AP2 DNA binding 449 G2583 gi12225884 1.10E−16 Zea mays unnamed protein product. 449 G2583 gi3264767 1.10E−16 Prunus armeniaca AP2 domain containing protein. 449 G2583 gi4099914 1.10E−16 Stylosanthes hamata ethylene-responsive element binding p 449 G2583 gi8809573 1.40E−16 Nicotiana sylvestris ethylene-responsive element binding 451 G2701 AW164275 3.00E−68 Glycine max se70d01.y1 Gm-c1023 Glycine max cDNA clone GENO 451 G2701 AF239956 2.00E−58 Hevea brasiliensis unknown mRNA. 451 G2701 BQ115848 3.00E−57 Solanum tuberosum EST601424 mixed potato tissues Solanum tu 451 G2701 AW220831 8.00E−53 Lycopersicon EST297300 tomato fruit esculentum mature green 451 G2701 BQ992139 4.00E−52 Lactuca sativa QGF24M24.yg.ab1 QG_EFGHJ lettuce serriola La 451 G2701 BE319813 4.00E−48 Medicago truncatula NF022C09RT1F1066 Developing root Medica 451 G2701 AAAA01017329 2.00E−46 Oryza sativa (indica ( ) scaffold017329 cultivar-group) 451 G2701 AC130612 2.00E−46 Oryza sativa (japonica ( ) chromosome 5 clo cultivar-group) 451 G2701 AP003279 3.00E−45 Oryza sativa chromosome 1 clone P0529E05, *** SEQUENCING IN 451 G2701 BG525326 1.00E−42 Stevia rebaudiana 48-70 Stevia field grown leaf cDNA Stevia 451 G2701 gi12005328 4.20E−56 Hevea brasiliensis unknown. 451 G2701 gi18874263 3.00E−55 Antirrhinum majus MYB-like transcription factor DIVARICAT 451 G2701 gi18461206 1.10E−48 Oryza sativa (japonica contains ESTs AU10 cultivar-group) 451 G2701 gi10798825 2.00E−45 Oryza sativa putative myb-related transcription activator 451 G2701 gi6688529 5.60E−45 Lycopersicon I-box binding factor. esculentum 451 G2701 gi19911579 4.00E−44 Glycine max syringolide-induced protein 1-3-1B. 451 G2701 gi15209176 9.20E−43 Solanum demissum putative I-box binding factor. 451 G2701 gi12406995 1.20E−26 Hordeum vulgare MCB2 protein. 451 G2701 gi7705206 7.60E−25 Solanum tuberosum MybSt1. 451 G2701 gi20067661 7.00E−15 Zea mays one repeat myb transcriptional factor. 453 G2719 BF097761 8.00E−50 Lycopersicon EST415834 tomato nutrient esculentum deficient 453 G2719 BQ995199 1.00E−49 Lactuca sativa QGF9F12.yg.ab1 QG_EFGHJ lettuce serriola Lac 453 G2719 CA785073 6.00E−48 Glycine max sat27b04.y1 Gm-c1056 Glycine max cDNA clone SOY 453 G2719 AW689391 1.00E−47 Medicago truncatula NF018F11ST1F1000 Developing stem Medica 453 G2719 BU025163 1.00E−45 Helianthus annuus QHF7P05.yg.ab1 QH_EFGHJ sunflower RHA280 453 G2719 AP004467 2.00E−43 Lotus japonicus genomic DNA, chromosome 1, clone: LjT06K11, 453 G2719 BH444284 6.00E−43 Brassica oleracea BOGON79TF BOGO Brassica oleracea genomic 453 G2719 AAAA01031778 2.00E−41 Oryza sativa (indica ( ) scaffold031778 cultivar-group) 453 G2719 BU875887 4.00E−41 Populus balsamifera V012F11 Populus flow subsp. trichocarpa 453 G2719 AP005821 9.00E−41 Oryza sativa (japonica ( ) chromosome 9 clo cultivar-group) 453 G2719 gi20160571 2.90E−64 Oryza sativa (japonica putative MYB trans cultivar-group) 453 G2719 gi9954112 6.70E−43 Solanum tuberosum tuber-specific and sucrose- responsive e 453 G2719 gi6539552 1.60E−33 Oryza sativa Similar to putative transcription factor (AF 453 G2719 gi7677136 7.80E−32 Adiantum raddianum c-myb-like transcription factor. 453 G2719 gi16326135 1.20E−31 Nicotiana tabacum Myb. 453 G2719 gi7230673 1.60E−31 Papaver rhoeas putative Myb-related domain. 453 G2719 gi1200239 8.50E−31 Hordeum vulgare GAMyb protein. 453 G2719 gi8745321 8.50E−31 Physcomitrella patens putative c-myb-like transcription f 453 G2719 gi20565 3.80E−30 Petunia x hybrida protein 3. 453 G2719 gi4581969 2.00E−29 Avena sativa myb protein. 455 G2789 BH975957 1.00E−77 Brassica oleracea odh67e11.g1 B. oleracea002 Brassica olerac 455 G2789 AJ502190 4.00E−76 Medicago truncatula AJ502190 MTAMP Medicago truncatula cDNA 455 G2789 AP005653 7.00E−68 Oryza sativa (japonica ( ) chromosome 2 clo cultivar-group) 455 G2789 AAAA01009427 7.00E−68 Oryza sativa (indica ( ) scaffold009427 cultivar-group) 455 G2789 BQ863249 1.00E−65 Lactuca sativa QGC23G02.yg.ab1 QG_ABCDI lettuce salinas Lac 455 G2789 AP003526 6.00E−64 Oryza sativa chromosome 6 clone P0548D03, *** SEQUENCING IN 455 G2789 BM110212 4.00E−62 Solanum tuberosum EST557748 potato roots Solanum tuberosum 455 G2789 BZ412041 2.00E−59 Zea mays OGACG56TC ZM_0.7_1.5_KB Zea mays genomic clone ZMM 455 G2789 BG134451 5.00E−59 Lycopersicon EST467343 tomato crown esculentum gall Lycoper 455 G2789 AP004971 4.00E−57 Lotus japonicus genomic DNA, chromosome 5, clone: LjT45G21, 455 G2789 gi15528814 5.70E−36 Oryza sativa hypothetical protein~similar to Arabidopsis 455 G2789 gi24059979 5.50E−31 Oryza sativa (japonica similar to DNA-bin cultivar-group) 455 G2789 gi4165183 4.50E−20 Antirrhinum majus SAP1 protein. 455 G2789 gi2213534 8.60E−19 Pisum sativum DNA-binding PD1-like protein. 455 G2789 gi14916565 0.98 Malus x domestica Flavonol synthase (FLS). 455 G2789 gi1313924 0.98 Brassica oleracea non intermediate filament IFA binding p 455 G2789 gi7671199 1 Chlamydomonas flagellar autotomy protein reinhardtii Fa1p 455 G2789 gi11466352 1 Mesostigma viride photosystem II protein N. 457 G2830 BH993354 7.00E−65 Brassica oleracea oeg99c11.g1 B. oleracea002 Brassica olerac 457 G2830 BM177052 3.00E−13 Glycine max saj76c01.y1 Gm-c1074 Glycine max cDNA clone SOY 457 G2830 BI137362 5.7 Populus balsamifera F084P95Y Populus flo subsp. trichocarpa 457 G2830 AC125368 5.7 Medicago truncatula clone mth2-13h15, WORKING DRAFT SEQUENC 457 G2830 BG269090 5.7 Mesembryanthemum L0-3090T3 Ice plant crystallinum Lambda Un 457 G2830 BE345092 7.5 Zea mays 946031F09.y1 946-tassel primordium prepared by S 457 G2830 gi8099397 0.97 Nicotiana tabacum protoporphyrinogen oxidase precursor; p

Table 9 lists sequences discovered to be paralogous to a number of transcription factors of the present invention. The columns headings include, from left to right, the Arabidopsis SEQ ID NO; corresponding Arabidopsis Gene ID (GID) numbers; the GID numbers of the paralogs discovered in a database search; and the SEQ ID NOs of the paralogs.

TABLE 9 Arabidopsis Transcription Factors and Paralogs SEQ ID GID NO: NO. Paralog SEQ ID NO: Paralog GID No. 8 G24 1952, 2090, 2104 G12, G1277, G1379 10 G28 2074 G1006 12 G47 408 G2133 16 G157 166, 350, 352 G859, G1842, G1843 20 G175 174 G877 32 G196 1962 G182 36 G214 146 G680 38 G226 148 G682 40 G241 1978 G233 44 G254 1974 G228 46 G256 2048, 2050, 2066 G666, G668, G932 48 G278 2092 G1290 50 G291 2088 G1211 56 G325 2160 G1998 58 G343 1986 G342 60 G353 62 G354 62 G354 60 G353 64 G361 66 G362 66 G362 64 G361 70 G390 72, 78 G391, G438 72 G391 70, 78 G390, G438 76 G427 1996, 1998, 2188 G425, G426, G2545 78 G438 70, 72 G390, G391 80 G450 2002, 2004, 2006 G448, G455, G456 82 G464 2008 G463 88 G481 90, 2010 G482, G485, G2345 90 G482 88, 2010 G481, G485 92 G484 2190 G2631 94 G489 2054 G714 98 G504 2108, 2110 G1425, G1454 102 G519 2012, 2014, 2060 G501, G502, G767 104 G545 1988, 1990 G350, G351 114 G568 2034 G580 116 G584 2082 G1136 118 G585 2036 G586 122 G594 294 G1496 136 G652 2096 G1335 138 G663 2094, 2174, 2176 G1329, G2421, G2422 140 G664 1964, 1984 G197, G255 144 G676 1966, 1980 G212, G247 146 G680 36 G214 148 G682 38, 1972, 2142, 2192 G225, G226, G1816, G2718 150 G715 314 G1646 154 G736 2182 G2432 160 G789 292 G1494 164 G849 2042 G610 166 G859 16, 350, 352, 2130, G157, G1842, G1843, G1759, 2146 G1844 170 G867 1950, 370 G9, G1930 174 G877 20 G175 176 G881 2068 G986 180 G896 2098 G1349 186 G912 1958, 1960, 1962 G40, G41, G42 188 G913 2162 G2107 194 G961 2186 G2535 198 G974 1948 G5 200 G975 450 G2583 202 G979 2164 G2131 204 G987 2202 G3010 208 G1040 2056, 2058 G729, G730 210 G1047 2140 G1808 212 G1501 214 G1052 214 G1052 212 G1051 216 G1062 2128 G1664 218 G1063 414 G2143 224 G1073 2078, 2166 G1067, G2156 226 G1075 2080 G1076 232 G1134 446 G2555 234 G1140 2064 G861 238 G1146 2084, 2086 G1149, G1152 240 G1196 2062 G839 242 G1198 2024, 2026, 2028, G554, G555, G556, G558, 2030, 2032, 2044, 2138 G578, G629, G1806 250 G1255 2122 G1484 258 G1322 1970, 1982 G221, G249 260 G1323 2046 G659 262 G1330 2178 G2423 268 G1363 2132 G1782 270 G1411 440 G2509 278 G1451 2070 G990 280 G1452 2016, 2100 G512, G1357 282 G1463 2114, 2116, 2118, 2120 G1461, G1462, G1464, G1465 286 G1478 2152 G1929 288 G1482 2148 G1888 292 G1494 160 G789 294 G1496 122 G594 306 G1634 452 G2701 312 G1645 2180 G2424 316 G1652 2194 G2776 322 G1749 2144 G1839 324 G1750 2000 G440 332 G1792 1954, 2134, 2136 G30, G1791, G1795 340 G1818 344 G1836 344 G1836 340 G1818 350 G1842 16, 166, 352 G157, G859, G1843 352 G1843 16, 166, 350 G157, G859, G1842 356 G1863 2170 G2334 360 G1895 364 G1903 364 G1903 360 G1895 368 G1927 2168 G2184 370 G1930 170 G867 374 G1944 2040 G605 386 G2007 1976 G231 388 G2010 432 G2347 390 G2053 2018, 2020, 2022 G515, G516, G517 406 G2130 2076 G1008 408 G2133 12 G47 414 G2143 218 G1063 420 G2155 2154 G1945 426 G2340 2052 G671 432 G2347 388 G2010 440 G2509 270 G1411 446 G2555 232 G1134 450 G2583 200 G975 452 G2701 306 G1634 454 G2719 1968 G216 456 G2789 2038 G596 1948 G5 198 G974 1950 G9 170, 370 G867, G1930 1952 G12 8 G24 1954 G30 332 G1792 1956 G40 1958, 1960, 186 G41, G42, G912 1958 G41 1956, 1960, 186 G40, G42, G912 1960 G42 1956, 1958, 186 G40, G41, G912 1962 G182 32 G196 1964 G197 140 G664 1966 G212 144 G676 1968 G216 454 G2719 1970 G221 258 G1322 1972 G225 38, 148 G226, G682 1974 G228 44 G254 1976 G231 386 G2007 1978 G233 40 G241 1980 G247 144 G676 1982 G249 258 G1322 1984 G255 140 G664 1986 G342 58 G343 1988 G350 104 G545 1990 G351 104 G545 1992 G370 64, 66 G361, G362 1994 G392 70, 72, 78 G390, G391, G438 1996 G425 76 G427 1998 G426 76 G427 2000 G440 324 G1750 2002 G448 80 G450 2004 G455 80 G450 2006 G456 80 G450 2008 G463 82 G464 2010 G485 88, 90 G481, G482 2012 G501 102 G519 2014 G502 102 G519 2016 G512 280 G1452 2018 G515 390 G2053 2020 G516 390 G2053 2022 G517 390 G2053 2024 G554 242 G1198 2026 G555 242 G1198 2028 G556 242 G1198 2030 G558 242 G1198 2032 G578 242 G1198 2034 G580 114 G568 2036 G586 118 G585 2038 G596 456 G2789 2040 G605 374 G1944 2042 G610 164 G849 2044 G629 242 G1198 2046 G659 260 G1323 2048 G666 46 G256 2050 G668 46 G256 2052 G671 426 G2340 2054 G714 94 G489 2056 G729 208 G1040 2058 G730 208 G1040 2060 G767 102 G519 2062 G839 240 G1196 2064 G861 234 G1140 2066 G932 46 G256 2068 G986 176 G881 2070 G990 278 G1451 2072 G993 170, 370 G867, G1930 2074 G1006 10 G28 2076 G1008 406 G2130 2078 G1067 224 G1073 2080 G1076 226 G1075 2082 G1136 116 G584 2084 G1149 238 G1146 2086 G1152 238 G1146 2088 G1211 50 G291 2090 G1277 8 G24 2092 G1290 48 G278 2094 G1329 138 G663 2096 G1335 136 G652 2098 G1349 180 G896 2100 G1357 280 G1452 2102 G1364 88, 90 G481, G482 2104 G1379 8 G24 2106 G1387 200, 450 G975, G2583 2108 G1425 98 G504 2110 G1454 98 G504 2114 G1461 282 G1463 2116 G1462 282 G1463 2118 G1464 282 G1463 2120 G1465 282 G1463 2122 G1484 250 G1255 2124 G1548 70, 72, 78 G390, G391, G438 2126 G1646 150 G715 2128 G1664 216 G1062 2130 G1759 16, 172, 350, 352 G157, G859, G1842, G1843 2132 G1782 268 G1363 2134 G1791 332 G1792 2136 G1795 332 G1792 2138 G1806 242 G1198 2140 G1808 210 G1047 2142 G1816 38, 148 G226, G682 2144 G1839 322 G1749 2146 G1844 16, 166, 350, 352 G157, G859, G1842, G1843 2148 G1888 288 G1482 2150 G1889 60, 62 G353, G354 2152 G1929 286 G1478 2154 G1945 420 G2155 2156 G1974 60, 62 G353, G354 2158 G1995 64, 66 G361, G362 2160 G1998 56 G325 2162 G2107 186, 188 G912, G913 2164 G2131 202 G979 2166 G2156 224 G1073 2168 G2184 368 G1927 2170 G2334 356 G1863 2172 G2345 88, 90 G481, G482 2174 G2421 138 G663 2176 G2422 138 G663 2178 G2423 262 G1330 2180 G2424 312 G1645 2182 G2432 154 G736 2184 G2513 1956, 1958, 1960, 186 G40, G41, G42, G912 2186 G2535 194 G961 2188 G2545 76 G427 2190 G2631 92 G484 2192 G2718 38, 148 G226, G682 2194 G2776 316 G1652 2196 G2826 64, 66 G361, G362, G1995 2198 G2838 64, 66 G361, G362, G1995 2200 G2839 60, 62 G353, G354 2202 G3010 204 G987

Table 10 lists the gene identification number (GID) and homologous relationships found using analyses according to Example IX for the sequences of the Sequence Listing.

TABLE 10 Homologous relationships found within the Sequence Listing Species DNA from Which SEQ or Homologous Relationship of ID GID Protein Sequence SEQ ID NO: NO: No. (PRT) is Derived to Other Genes 459 DNA Glycine max Predicted polypeptide sequence is orthologous to G8 460 DNA Glycine max Predicted polypeptide sequence is orthologous to G8 461 DNA Glycine max Predicted polypeptide sequence is orthologous to G8 462 DNA Glycine max Predicted polypeptide sequence is orthologous to G8 463 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G8 464 DNA Zea mays Predicted polypeptide sequence is orthologous to G8 465 DNA Zea mays Predicted polypeptide sequence is orthologous to G8 466 DNA Zea mays Predicted polypeptide sequence is orthologous to G8 467 PRT Oryza sativa Orthologous to G8 468 DNA Glycine max Predicted polypeptide sequence is orthologous to G19 469 DNA Glycine max Predicted polypeptide sequence is orthologous to G19 470 DNA Glycine max Predicted polypeptide sequence is orthologous to G19 471 DNA Glycine max Predicted polypeptide sequence is orthologous to G19 472 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G19 473 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G19 474 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G19 475 DNA Zea mays Predicted polypeptide sequence is orthologous to G19 476 DNA Zea mays Predicted polypeptide sequence is orthologous to G19 477 DNA Glycine max Predicted polypeptide sequence is orthologous to G22 478 DNA Glycine max Predicted polypeptide sequence is orthologous to G22 479 DNA Glycine max Predicted polypeptide sequence is orthologous to G24 480 DNA Glycine max Predicted polypeptide sequence is orthologous to G24 481 DNA Glycine max Predicted polypeptide sequence is orthologous to G24 482 DNA Glycine max Predicted polypeptide sequence is orthologous to G24 483 DNA Glycine max Predicted polypeptide sequence is orthologous to G24 484 DNA Glycine max Predicted polypeptide sequence is orthologous to G24 485 DNA Glycine max Predicted polypeptide sequence is orthologous to G24 486 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G24 487 DNA Zea mays Predicted polypeptide sequence is orthologous to G24 488 PRT Oryza sativa Orthologous to G24 489 PRT Oryza sativa Orthologous to G24 490 PRT Oryza sativa Orthologous to G24 491 DNA Glycine max Predicted polypeptide sequence is orthologous to G28 492 DNA Glycine max Predicted polypeptide sequence is orthologous to G28 493 DNA Glycine max Predicted polypeptide sequence is orthologous to G28 494 DNA Glycine max Predicted polypeptide sequence is orthologous to G28 495 DNA Glycine max Predicted polypeptide sequence is orthologous to G28 496 DNA Glycine max Predicted polypeptide sequence is orthologous to G28 497 DNA Glycine max Predicted polypeptide sequence is orthologous to G28 498 DNA Glycine max Predicted polypeptide sequence is orthologous to G28 499 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G28 500 DNA Zea mays Predicted polypeptide sequence is orthologous to G28 501 PRT Oryza sativa Orthologous to G28 502 PRT Oryza sativa Orthologous to G28 503 PRT Mesembryanthemum Orthologous to G28 crystallinum 504 DNA Glycine max Predicted polypeptide sequence is orthologous to G47, G2133 505 PRT Oryza sativa Orthologous to G47, G2133 506 DNA Glycine max Predicted polypeptide sequence is orthologous to G157, G859, G1842, G1843 507 DNA Glycine max Predicted polypeptide sequence is orthologous to G175, G877 508 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G175, G877 509 DNA Zea mays Predicted polypeptide sequence is orthologous to G175, G877 510 DNA Zea mays Predicted polypeptide sequence is orthologous to G175, G877 511 DNA Zea mays Predicted polypeptide sequence is orthologous to G175, G877 512 PRT Oryza sativa Orthologous to G175, G877 513 PRT Oryza sativa Orthologous to G175, G877 514 PRT Oryza sativa Orthologous to G175, G877 515 PRT Nicotiana tabacum Orthologous to G175, G877 516 DNA Glycine max Predicted polypeptide sequence is orthologous to G180 517 DNA Glycine max Predicted polypeptide sequence is orthologous to G180 518 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G180 519 DNA Zea mays Predicted polypeptide sequence is orthologous to G180 520 DNA Solanum tuberosum Predicted polypeptide sequence is orthologous to G180 521 PRT Oryza sativa Orthologous to G180 522 PRT Capsella rubella Orthologous to G183 523 DNA Glycine max Predicted polypeptide sequence is orthologous to G188 524 DNA Zea mays Predicted polypeptide sequence is orthologous to G188 525 PRT Oryza sativa Orthologous to G188 526 PRT Oryza sativa Orthologous to G188 527 DNA Glycine max Predicted polypeptide sequence is orthologous to G189 528 PRT Nicotiana tabacum Orthologous to G189 529 DNA Glycine max Predicted polypeptide sequence is orthologous to G192 530 PRT Oryza sativa Orthologous to G192 531 DNA Glycine max Predicted polypeptide sequence is orthologous to G196 532 DNA Zea mays Predicted polypeptide sequence is orthologous to G196 533 DNA Zea mays Predicted polypeptide sequence is orthologous to G196 534 PRT Oryza sativa Orthologous to G196 535 PRT Oryza sativa Orthologous to G196 536 PRT Oryza sativa Orthologous to G196 537 PRT Oryza sativa Orthologous to G196 538 DNA Glycine max Predicted polypeptide sequence is orthologous to G211 539 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G211 540 PRT Oryza sativa Orthologous to G211 541 DNA Glycine max Predicted polypeptide sequence is orthologous to G214, G680 542 DNA Glycine max Predicted polypeptide sequence is orthologous to G214, G680 543 DNA Glycine max Predicted polypeptide sequence is orthologous to G214, G680 544 DNA Glycine max Predicted polypeptide sequence is orthologous to G214, G680 545 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G214, G680 546 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G214, G680 547 DNA Zea mays Predicted polypeptide sequence is orthologous to G214, G680 548 DNA Zea mays Predicted polypeptide sequence is orthologous to G214, G680 549 DNA Zea mays Predicted polypeptide sequence is orthologous to G214, G680 550 DNA Glycine max Predicted polypeptide sequence is orthologous to G226, G682 551 DNA Glycine max Predicted polypeptide sequence is orthologous to G226 552 DNA Glycine max Predicted polypeptide sequence is orthologous to G226, G682 553 DNA Glycine max Predicted polypeptide sequence is orthologous to G226, G682 554 DNA Glycine max Predicted polypeptide sequence is orthologous to G226, G682 555 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G226, G682 556 DNA Zea mays Predicted polypeptide sequence is orthologous to G226, G682 557 DNA Zea mays Predicted polypeptide sequence is orthologous to G226, G682 558 PRT Oryza sativa Orthologous to G226, G682 559 PRT Oryza sativa Orthologous to G226, G682 560 DNA Glycine max Predicted polypeptide sequence is orthologous to G241 561 DNA Glycine max Predicted polypeptide sequence is orthologous to G241 562 DNA Glycine max Predicted polypeptide sequence is orthologous to G241 563 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G241 564 DNA Zea mays Predicted polypeptide sequence is orthologous to G241 565 DNA Zea mays Predicted polypeptide sequence is orthologous to G241 566 DNA Zea mays Predicted polypeptide sequence is orthologous to G241 567 DNA Zea mays Predicted polypeptide sequence is orthologous to G241 568 DNA Zea mays Predicted polypeptide sequence is orthologous to G241 569 PRT Nicotiana tabacum Orthologous to G241 570 DNA Glycine max Predicted polypeptide sequence is orthologous to G254 571 DNA Glycine max Predicted polypeptide sequence is orthologous to G256 572 DNA Glycine max Predicted polypeptide sequence is orthologous to G256 573 DNA Glycine max Predicted polypeptide sequence is orthologous to G256 574 DNA Glycine max Predicted polypeptide sequence is orthologous to G256 575 DNA Glycine max Predicted polypeptide sequence is orthologous to G256 576 DNA Glycine max Predicted polypeptide sequence is orthologous to G256 577 DNA Glycine max Predicted polypeptide sequence is orthologous to G256 578 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G256 579 DNA Zea mays Predicted polypeptide sequence is orthologous to G256 580 DNA Zea mays Predicted polypeptide sequence is orthologous to G256 581 DNA Zea mays Predicted polypeptide sequence is orthologous to G256 582 DNA Zea mays Predicted polypeptide sequence is orthologous to G256 583 DNA Zea mays Predicted polypeptide sequence is orthologous to G256 584 DNA Zea mays Predicted polypeptide sequence is orthologous to G256 585 G3500 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G256 586 G3501 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G256 587 G3385 PRT Oryza sativa Orthologous to G256 588 G3386 PRT Oryza sativa Orthologous to G256 589 PRT Oryza sativa Orthologous to G256 590 G3384 PRT Oryza sativa Orthologous to G256 591 PRT Oryza sativa Orthologous to G256 592 G3502 PRT Oryza sativa Orthologous to G256 japonica 593 G3500 PRT Lycopersicon Orthologous to G256 esculentum 594 G3501 PRT Lycopersicon Orthologous to G256 esculentum 595 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G278 596 DNA Zea mays Predicted polypeptide sequence is orthologous to G278 597 PRT Oryza sativa Orthologous to G278 598 DNA Glycine max Predicted polypeptide sequence is orthologous to G312 599 DNA Zea mays Predicted polypeptide sequence is orthologous to G312 600 DNA Euphorbia esula Predicted polypeptide sequence is orthologous to G312 601 DNA Glycine max Predicted polypeptide sequence is orthologous to G325 602 DNA Glycine max Predicted polypeptide sequence is orthologous to G343 603 DNA Glycine max Predicted polypeptide sequence is orthologous to G343 604 DNA Glycine max Predicted polypeptide sequence is orthologous to G343 605 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G343 606 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G343 607 PRT Oryza sativa Orthologous to G343 608 PRT Oryza sativa Orthologous to G343 609 PRT Oryza sativa Orthologous to G343 610 DNA Glycine max Predicted polypeptide sequence is orthologous to G353, G354 611 DNA Glycine max Predicted polypeptide sequence is orthologous to G353, G354 612 DNA Glycine max Predicted polypeptide sequence is orthologous to G353, G354 613 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G353, G354 614 DNA Zea mays Predicted polypeptide sequence is orthologous to G353, G354 615 DNA Zea mays Predicted polypeptide sequence is orthologous to G353, G354 616 DNA Zea mays Predicted polypeptide sequence is orthologous to G353, G354 617 DNA Zea mays Predicted polypeptide sequence is orthologous to G353, G354 618 DNA Zea mays Predicted polypeptide sequence is orthologous to G353, G354 619 DNA Zea mays Predicted polypeptide sequence is orthologous to G353, G354 620 DNA Zea mays Predicted polypeptide sequence is orthologous to G353, G354 621 PRT Oryza sativa Orthologous to G353, G354 622 PRT Oryza sativa Orthologous to G353, G354 623 PRT Oryza sativa Orthologous to G353, G354 624 PRT Oryza sativa Orthologous to G353, G354 625 PRT Oryza sativa Orthologous to G353, G354 626 PRT Oryza sativa Orthologous to G353, G354 627 DNA Glycine max Predicted polypeptide sequence is orthologous to G361, G362 628 DNA Glycine max Predicted polypeptide sequence is orthologous to G361, G362 629 DNA Glycine max Predicted polypeptide sequence is orthologous to G361 630 DNA Glycine max Predicted polypeptide sequence is orthologous to G361, G362 631 DNA Glycine max Predicted polypeptide sequence is orthologous to G361, G362 632 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G361, G362 633 DNA Zea mays Predicted polypeptide sequence is orthologous to G361, G362 634 DNA Zea mays Predicted polypeptide sequence is orthologous to G361, G362 635 PRT Oryza sativa Orthologous to G361, G362 636 PRT Oryza sativa Orthologous to G361, G362 637 PRT Oryza sativa Orthologous to G361, G362 638 PRT Oryza sativa Orthologous to G361, G362 639 PRT Oryza sativa Orthologous to G361, G362 640 DNA Glycine max Predicted polypeptide sequence is orthologous to G390, G391, G438 641 DNA Glycine max Predicted polypeptide sequence is orthologous to G390, G391, G438 642 DNA Glycine max Predicted polypeptide sequence is orthologous to G390, G391, G438 643 DNA Glycine max Predicted polypeptide sequence is orthologous to G390, G391, G438 644 DNA Glycine max Predicted polypeptide sequence is orthologous to G390, G391, G438 645 DNA Glycine max Predicted polypeptide sequence is orthologous to G390, G391, G438 646 DNA Glycine max Predicted polypeptide sequence is orthologous to G390, G391, G438 647 DNA Glycine max Predicted polypeptide sequence is orthologous to G390, G391 648 DNA Glycine max Predicted polypeptide sequence is orthologous to G390, G391, G438 649 DNA Glycine max Predicted polypeptide sequence is orthologous to G390, G391, G438 650 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G390 651 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G390, G438 652 DNA Zea mays Predicted polypeptide sequence is orthologous to G390, G391, G438 653 DNA Zea mays Predicted polypeptide sequence is orthologous to G390, G391, G438 654 DNA Zea mays Predicted polypeptide sequence is orthologous to G390, G391, G438 655 DNA Zea mays Predicted polypeptide sequence is orthologous to G390, G391 656 DNA Zea mays Predicted polypeptide sequence is orthologous to G390, G391, G438 657 DNA Zea mays Predicted polypeptide sequence is orthologous to G390, G391, G438 658 DNA Zea mays Predicted polypeptide sequence is orthologous to G390, G391, G438 659 DNA Zea mays Predicted polypeptide sequence is orthologous to G390, G391, G438 660 DNA Zea mays Predicted polypeptide sequence is orthologous to G390, G391, G438 661 DNA Zea mays Predicted polypeptide sequence is orthologous to G390, G391, G438 662 DNA Zea mays Predicted polypeptide sequence is orthologous to G390, G391, G438 663 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G390, G391, G438 664 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G391, G438 665 PRT Oryza sativa Orthologous to G390, G391, G438 666 PRT Oryza sativa Orthologous to G390, G391, G438 667 PRT Oryza sativa Orthologous to G390, G391, G438 668 PRT Oryza sativa Orthologous to G390, G391, G438 669 PRT Physcomitrella Orthologous to G391 patens 670 DNA Glycine max Predicted polypeptide sequence is orthologous to G409 671 DNA Glycine max Predicted polypeptide sequence is orthologous to G409 672 DNA Glycine max Predicted polypeptide sequence is orthologous to G409 673 DNA Glycine max Predicted polypeptide sequence is orthologous to G409 674 DNA Glycine max Predicted polypeptide sequence is orthologous to G409 675 DNA Glycine max Predicted polypeptide sequence is orthologous to G409 676 DNA Glycine max Predicted polypeptide sequence is orthologous to G409 677 DNA Glycine max Predicted polypeptide sequence is orthologous to G409 678 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G409 679 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G409 680 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G409 681 DNA Zea mays Predicted polypeptide sequence is orthologous to G409 682 DNA Zea mays Predicted polypeptide sequence is orthologous to G409 683 DNA Zea mays Predicted polypeptide sequence is orthologous to G409 684 DNA Zea mays Predicted polypeptide sequence is orthologous to G409 685 DNA Zea mays Predicted polypeptide sequence is orthologous to G409 686 DNA Zea mays Predicted polypeptide sequence is orthologous to G409 687 DNA Zea mays Predicted polypeptide sequence is orthologous to G409 688 DNA Glycine max Predicted polypeptide sequence is orthologous to G427 689 DNA Glycine max Predicted polypeptide sequence is orthologous to G427 690 DNA Glycine max Predicted polypeptide sequence is orthologous to G427 691 DNA Glycine max Predicted polypeptide sequence is orthologous to G427 692 DNA Glycine max Predicted polypeptide sequence is orthologous to G427 693 DNA Glycine max Predicted polypeptide sequence is orthologous to G427 694 DNA Glycine max Predicted polypeptide sequence is orthologous to G427 695 DNA Glycine max Predicted polypeptide sequence is orthologous to G427 696 DNA Glycine max Predicted polypeptide sequence is orthologous to G427 697 DNA Glycine max Predicted polypeptide sequence is orthologous to G427 698 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G427 699 DNA Zea mays Predicted polypeptide sequence is orthologous to G427 700 DNA Zea mays Predicted polypeptide sequence is orthologous to G427 701 DNA Zea mays Predicted polypeptide sequence is orthologous to G427 702 DNA Zea mays Predicted polypeptide sequence is orthologous to G427 703 DNA Zea mays Predicted polypeptide sequence is orthologous to G427 704 DNA Zea mays Predicted polypeptide sequence is orthologous to G427 705 DNA Zea mays Predicted polypeptide sequence is orthologous to G427 706 DNA Zea mays Predicted polypeptide sequence is orthologous to G427 707 DNA Zea mays Predicted polypeptide sequence is orthologous to G427 708 PRT Oryza sativa Orthologous to G427 709 PRT Oryza sativa Orthologous to G427 710 PRT Oryza sativa Orthologous to G427 711 PRT Malus x domestica Orthologous to G427 712 PRT Nicotiana tabacum Orthologous to G427 713 PRT Lycopersicon Orthologous to G427 esculentum 714 DNA Glycine max Predicted polypeptide sequence is orthologous to G438 715 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G438 716 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G438 717 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G438 718 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G438 719 DNA Zea mays Predicted polypeptide sequence is orthologous to G438 720 PRT Physcomitrella patens Orthologous to G438 721 PRT Oryza sativa Orthologous to G438 722 DNA Glycine max Predicted polypeptide sequence is orthologous to G450 723 DNA Glycine max Predicted polypeptide sequence is orthologous to G450 724 DNA Glycine max Predicted polypeptide sequence is orthologous to G450 725 DNA Glycine max Predicted polypeptide sequence is orthologous to G450 726 DNA Glycine max Predicted polypeptide sequence is orthologous to G450 727 DNA Glycine max Predicted polypeptide sequence is orthologous to G450 728 DNA Glycine max Predicted polypeptide sequence is orthologous to G450 729 DNA Glycine max Predicted polypeptide sequence is orthologous to G450 730 DNA Glycine max Predicted polypeptide sequence is orthologous to G450 731 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G450 732 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G450 733 DNA Zea mays Predicted polypeptide sequence is orthologous to G450 734 DNA Zea mays Predicted polypeptide sequence is orthologous to G450 735 DNA Zea mays Predicted polypeptide sequence is orthologous to G450 736 PRT Oryza sativa Orthologous to G450 737 PRT Oryza sativa Orthologous to G450 738 PRT Oryza sativa Orthologous to G450 739 PRT Oryza sativa Orthologous to G450 740 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G464 741 DNA Zea mays Predicted polypeptide sequence is orthologous to G464 742 PRT Oryza sativa Orthologous to G464 743 DNA Glycine max Predicted polypeptide sequence is orthologous to G470 744 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G470 745 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G470 746 DNA Glycine max Predicted polypeptide sequence is orthologous to G481, G482 747 DNA Glycine max Predicted polypeptide sequence is orthologous to G481, G482 748 DNA Glycine max Predicted polypeptide sequence is orthologous to G481, G482 749 DNA Glycine max Predicted polypeptide sequence is orthologous to G481, G482 750 DNA Glycine max Predicted polypeptide sequence is orthologous to G481, G482 751 DNA Glycine max Predicted polypeptide sequence is orthologous to G481, G482 752 DNA Glycine max Predicted polypeptide sequence is orthologous to G481, G482 753 DNA Glycine max Predicted polypeptide sequence is orthologous to G481, G482 754 DNA Glycine max Predicted polypeptide sequence is orthologous to G481 755 DNA Glycine max Predicted polypeptide sequence is orthologous to G481 756 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G481 757 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G481, G482 758 DNA Zea mays Predicted polypeptide sequence is orthologous to G481 759 DNA Zea mays Predicted polypeptide sequence is orthologous to G481, G482 760 DNA Zea mays Predicted polypeptide sequence is orthologous to G481, G482 761 DNA Zea mays Predicted polypeptide sequence is orthologous to G481, G482 762 DNA Zea mays Predicted polypeptide sequence is orthologous to G481, G482 763 DNA Zea mays Predicted polypeptide sequence is orthologous to G481, G482 764 DNA Zea mays Predicted polypeptide sequence is orthologous to G481, G482 765 DNA Zea mays Predicted polypeptide sequence is orthologous to G481, G482 766 DNA Zea mays Predicted polypeptide sequence is orthologous to G481, G482 767 DNA Zea mays Predicted polypeptide sequence is orthologous to G481, G482 768 DNA Gossypium arboreum Predicted polypeptide sequence is orthologous to G481, G482 769 DNA Glycine max Predicted polypeptide sequence is orthologous to G481, G482 770 DNA Gossypium hirsutum Predicted polypeptide sequence is orthologous to G481, G482 771 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G481, G482 772 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G481, G482 773 DNA Medicago truncatula Predicted polypeptide sequence is orthologous to G481, G482 774 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G481, G482 775 DNA Solanum tuberosum Predicted polypeptide sequence is orthologous to G481, G482 776 DNA Triticum aestivum Predicted polypeptide sequence is orthologous to G481, G482 777 DNA Hordeum vulgare Predicted polypeptide sequence is orthologous to G481, G482 778 DNA Triticum monococcum Predicted polypeptide sequence is orthologous to G481, G482 779 DNA Glycine max Predicted polypeptide sequence is orthologous to G482 780 PRT Oryza sativa Orthologous to G481, G482 781 PRT Oryza sativa Orthologous to G481, G482 782 PRT Oryza sativa Orthologous to G481, G482 783 PRT Oryza sativa Orthologous to G481, G482 784 PRT Oryza sativa Orthologous to G481, G482 785 PRT Zea mays Orthologous to G481, G482 786 PRT Zea mays Orthologous to G481, G482 787 PRT Oryza sativa Orthologous to G481, G482 788 PRT Oryza sativa Orthologous to G481, G482 789 PRT Oryza sativa Orthologous to G481, G482 790 PRT Oryza sativa Orthologous to G481, G482 791 PRT Oryza sativa Orthologous to G481, G482 792 PRT Oryza sativa Orthologous to G481, G482 793 PRT Oryza sativa Orthologous to G481, G482 794 PRT Oryza sativa Orthologous to G481, G482 795 PRT Oryza sativa Orthologous to G481, G482 796 PRT Oryza sativa Orthologous to G481, G482 797 PRT Glycine max Orthologous to G481, G482 798 PRT Glycine max Orthologous to G481, G482 799 PRT Glycine max Orthologous to G481, G482 800 PRT Glycine max Orthologous to G481, G482 801 PRT Glycine max Orthologous to G481, G482 802 PRT Glycine max Orthologous to G481, G482 803 PRT Glycine max Orthologous to G481, G482 804 PRT Zea mays Orthologous to G481, G482 805 PRT Zea mays Orthologous to G481, G482 806 PRT Zea mays Orthologous to G481, G482 807 PRT Zea mays Orthologous to G481, G482 808 DNA Glycine max Predicted polypeptide sequence is orthologous to G484 809 DNA Glycine max Predicted polypeptide sequence is orthologous to G484 810 DNA Glycine max Predicted polypeptide sequence is orthologous to G484 811 DNA Glycine max Predicted polypeptide sequence is orthologous to G484 812 DNA Glycine max Predicted polypeptide sequence is orthologous to G484 813 DNA Glycine max Predicted polypeptide sequence is orthologous to G484 814 DNA Glycine max Predicted polypeptide sequence is orthologous to G484 815 DNA Glycine max Predicted polypeptide sequence is orthologous to G484 816 DNA Glycine max Predicted polypeptide sequence is orthologous to G484 817 DNA Glycine max Predicted polypeptide sequence is orthologous to G484 818 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G484 819 DNA Zea mays Predicted polypeptide sequence is orthologous to G484 820 DNA Zea mays Predicted polypeptide sequence is orthologous to G484 821 DNA Zea mays Predicted polypeptide sequence is orthologous to G484 822 DNA Zea mays Predicted polypeptide sequence is orthologous to G484 823 DNA Zea mays Predicted polypeptide sequence is orthologous to G484 824 PRT Oryza sativa Orthologous to G484 825 DNA Glycine max Predicted polypeptide sequence is orthologous to G489 826 DNA Glycine max Predicted polypeptide sequence is orthologous to G489 827 DNA Glycine max Predicted polypeptide sequence is orthologous to G489 828 DNA Glycine max Predicted polypeptide sequence is orthologous to G489 829 DNA Glycine max Predicted polypeptide sequence is orthologous to G489 830 DNA Glycine max Predicted polypeptide sequence is orthologous to G489 831 DNA Glycine max Predicted polypeptide sequence is orthologous to G489 832 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G489 833 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G489 834 DNA Zea mays Predicted polypeptide sequence is orthologous to G489 835 PRT Oryza sativa Orthologous to G489 836 PRT Oryza sativa Orthologous to G489 837 PRT Oryza sativa Orthologous to G489 838 DNA Glycine max Predicted polypeptide sequence is orthologous to G504 839 DNA Glycine max Predicted polypeptide sequence is orthologous to G504 840 DNA Glycine max Predicted polypeptide sequence is orthologous to G504 841 DNA Glycine max Predicted polypeptide sequence is orthologous to G504 842 DNA Glycine max Predicted polypeptide sequence is orthologous to G504 843 DNA Glycine max Predicted polypeptide sequence is orthologous to G504 844 DNA Glycine max Predicted polypeptide sequence is orthologous to G504 845 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G504 846 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G504 847 DNA Zea mays Predicted polypeptide sequence is orthologous to G504 848 DNA Zea mays Predicted polypeptide sequence is orthologous to G504 849 DNA Zea mays Predicted polypeptide sequence is orthologous to G504 850 DNA Zea mays Predicted polypeptide sequence is orthologous to G504 851 PRT Oryza sativa Orthologous to G504 852 PRT Oryza sativa Orthologous to G504 853 PRT Oryza sativa Orthologous to G504 854 PRT Oryza sativa Orthologous to G504 855 DNA Lycopersicon Predicted polypeptide sequence is esculentum orthologous to G509 856 DNA Glycine max Predicted polypeptide sequence is orthologous to G509 857 DNA Glycine max Predicted polypeptide sequence is orthologous to G509 858 DNA Glycine max Predicted polypeptide sequence is orthologous to G509 859 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G509 860 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G509 861 DNA Zea mays Predicted polypeptide sequence is orthologous to G509 862 DNA Zea mays Predicted polypeptide sequence is orthologous to G509 863 DNA Zea mays Predicted polypeptide sequence is orthologous to G509 864 DNA Zea mays Predicted polypeptide sequence is orthologous to G509 865 PRT Oryza sativa Orthologous to G509 866 PRT Oryza sativa Orthologous to G509 867 PRT Oryza sativa Orthologous to G509 868 DNA Glycine max Predicted polypeptide sequence is orthologous to G519 869 DNA Glycine max Predicted polypeptide sequence is orthologous to G519 870 DNA Glycine max Predicted polypeptide sequence is orthologous to G519 871 DNA Glycine max Predicted polypeptide sequence is orthologous to G519 872 DNA Glycine max Predicted polypeptide sequence is orthologous to G519 873 DNA Glycine max Predicted polypeptide sequence is orthologous to G519 874 DNA Glycine max Predicted polypeptide sequence is orthologous to G519 875 DNA Glycine max Predicted polypeptide sequence is orthologous to G519 876 DNA Glycine max Predicted polypeptide sequence is orthologous to G519 877 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G519 878 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G519 879 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G519 880 DNA Zea mays Predicted polypeptide sequence is orthologous to G519 881 DNA Zea mays Predicted polypeptide sequence is orthologous to G519 882 DNA Zea mays Predicted polypeptide sequence is orthologous to G519 883 DNA Zea mays Predicted polypeptide sequence is orthologous to G519 884 DNA Zea mays Predicted polypeptide sequence is orthologous to G519 885 DNA Zea mays Predicted polypeptide sequence is orthologous to G519 886 DNA Zea mays Predicted polypeptide sequence is orthologous to G519 887 DNA Zea mays Predicted polypeptide sequence is orthologous to G519 888 DNA Zea mays Predicted polypeptide sequence is orthologous to G519 889 DNA Zea mays Predicted polypeptide sequence is orthologous to G519 890 PRT Oryza sativa Orthologous to G519 891 PRT Oryza sativa Orthologous to G519 892 DNA Glycine max Predicted polypeptide sequence is orthologous to G545 893 DNA Glycine max Predicted polypeptide sequence is orthologous to G545 894 DNA Glycine max Predicted polypeptide sequence is orthologous to G545 895 DNA Glycine max Predicted polypeptide sequence is orthologous to G545 896 DNA Glycine max Predicted polypeptide sequence is orthologous to G545 897 DNA Glycine max Predicted polypeptide sequence is orthologous to G545 898 DNA Glycine max Predicted polypeptide sequence is orthologous to G545 899 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G545 900 DNA Zea mays Predicted polypeptide sequence is orthologous to G545 901 DNA Zea mays Predicted polypeptide sequence is orthologous to G545 902 DNA Zea mays Predicted polypeptide sequence is orthologous to G545 903 PRT Oryza sativa Orthologous to G545 904 PRT Oryza sativa Orthologous to G545 905 PRT Oryza sativa Orthologous to G545 906 PRT Oryza sativa Orthologous to G545 907 PRT Datisca glomerata Orthologous to G545 908 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G546 909 DNA Zea mays Predicted polypeptide sequence is orthologous to G561 910 PRT Sinapis alba Orthologous to G561 911 PRT Raphanus sativus Orthologous to G561 912 PRT Brassica napus Orthologous to G561 913 PRT Brassica napus Orthologous to G561 914 DNA Glycine max Predicted polypeptide sequence is orthologous to G562 915 DNA Glycine max Predicted polypeptide sequence is orthologous to G562 916 DNA Glycine max Predicted polypeptide sequence is orthologous to G562 917 DNA Glycine max Predicted polypeptide sequence is orthologous to G562 918 DNA Glycine max Predicted polypeptide sequence is orthologous to G562 919 DNA Zea mays Predicted polypeptide sequence is orthologous to G562 920 DNA Zea mays Predicted polypeptide sequence is orthologous to G562 921 DNA Zea mays Predicted polypeptide sequence is orthologous to G562 922 PRT Oryza sativa Orthologous to G562 923 PRT Oryza sativa Orthologous to G562 924 DNA Glycine max Predicted polypeptide sequence is orthologous to G567 925 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G567 926 PRT Oryza sativa Orthologous to G567 927 DNA Glycine max Predicted polypeptide sequence is orthologous to G568 928 DNA Glycine max Predicted polypeptide sequence is orthologous to G568 929 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G568 930 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G568 931 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G568 932 DNA Zea mays Predicted polypeptide sequence is orthologous to G568 933 PRT Oryza sativa Orthologous to G568 934 PRT Populus balsamifera Orthologous to G568 subsp. trichocarpa x Populus deltoides 935 DNA Glycine max Predicted polypeptide sequence is orthologous to G584 936 DNA Glycine max Predicted polypeptide sequence is orthologous to G584 937 DNA Glycine max Predicted polypeptide sequence is orthologous to G584 938 DNA Glycine max Predicted polypeptide sequence is orthologous to G584 939 DNA Glycine max Predicted polypeptide sequence is orthologous to G584 940 DNA Zea mays Predicted polypeptide sequence is orthologous to G584 941 DNA Zea mays Predicted polypeptide sequence is orthologous to G584 942 DNA Zea mays Predicted polypeptide sequence is orthologous to G584 943 PRT Oryza sativa Orthologous to G584 944 DNA Glycine max Predicted polypeptide sequence is orthologous to G585 945 DNA Glycine max Predicted polypeptide sequence is orthologous to G585 946 DNA Glycine max Predicted polypeptide sequence is orthologous to G585 947 DNA Glycine max Predicted polypeptide sequence is orthologous to G585 948 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G585 949 DNA Zea mays Predicted polypeptide sequence is orthologous to G585 950 DNA Zea mays Predicted polypeptide sequence is orthologous to G585 951 DNA Zea mays Predicted polypeptide sequence is orthologous to G585 952 DNA Zea mays Predicted polypeptide sequence is orthologous to G585 953 PRT Oryza sativa Orthologous to G585 954 PRT Oryza sativa Orthologous to G585 955 PRT Oryza sativa Orthologous to G585 956 PRT Oryza sativa Orthologous to G585 957 PRT Oryza sativa Orthologous to G585 958 PRT Oryza sativa Orthologous to G585 959 PRT Gossypium hirsutum Orthologous to G585 960 PRT Antirrhinum majus Orthologous to G585 961 DNA Glycine max Predicted polypeptide sequence is orthologous to G590 962 DNA Glycine max Predicted polypeptide sequence is orthologous to G590 963 DNA Glycine max Predicted polypeptide sequence is orthologous to G590 964 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G590 965 DNA Zea mays Predicted polypeptide sequence is orthologous to G590 966 PRT Oryza sativa Orthologous to G590 967 PRT Oryza sativa Orthologous to G590 968 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G597 969 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G597 970 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G597 971 DNA Zea mays Predicted polypeptide sequence is orthologous to G597 972 DNA Zea mays Predicted polypeptide sequence is orthologous to G597 973 DNA Zea mays Predicted polypeptide sequence is orthologous to G597 974 DNA Zea mays Predicted polypeptide sequence is orthologous to G597 975 DNA Zea mays Predicted polypeptide sequence is orthologous to G597 976 DNA Zea mays Predicted polypeptide sequence is orthologous to G597 977 DNA Zea mays Predicted polypeptide sequence is orthologous to G597 978 DNA Zea mays Predicted polypeptide sequence is orthologous to G597 979 DNA Zea mays Predicted polypeptide sequence is orthologous to G597 980 DNA Zea mays Predicted polypeptide sequence is orthologous to G597 981 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G634 982 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G634 983 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G634 984 DNA Zea mays Predicted polypeptide sequence is orthologous to G634 985 DNA Zea mays Predicted polypeptide sequence is orthologous to G634 986 DNA Zea mays Predicted polypeptide sequence is orthologous to G634 987 PRT Oryza sativa Orthologous to G634 988 PRT Oryza sativa Orthologous to G634 989 DNA Glycine max Predicted polypeptide sequence is orthologous to G635 990 DNA Glycine max Predicted polypeptide sequence is orthologous to G635 991 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G635 992 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G635 993 DNA Zea mays Predicted polypeptide sequence is orthologous to G635 994 PRT Oryza sativa Orthologous to G635 995 DNA Glycine max Predicted polypeptide sequence is orthologous to G636 996 DNA Glycine max Predicted polypeptide sequence is orthologous to G636 997 DNA Glycine max Predicted polypeptide sequence is orthologous to G636 998 DNA Glycine max Predicted polypeptide sequence is orthologous to G636 999 DNA Glycine max Predicted polypeptide sequence is orthologous to G636 1000 DNA Glycine max Predicted polypeptide sequence is orthologous to G636 1001 DNA Glycine max Predicted polypeptide sequence is orthologous to G636 1002 DNA Glycine max Predicted polypeptide sequence is orthologous to G636 1003 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G636 1004 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G636 1005 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G636 1006 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G636 1007 DNA Zea mays Predicted polypeptide sequence is orthologous to G636 1008 DNA Zea mays Predicted polypeptide sequence is orthologous to G636 1009 DNA Zea mays Predicted polypeptide sequence is orthologous to G636 1010 DNA Zea mays Predicted polypeptide sequence is orthologous to G636 1011 PRT Pisum sativum Orthologous to G636 1012 DNA Glycine max Predicted polypeptide sequence is orthologous to G638 1013 DNA Glycine max Predicted polypeptide sequence is orthologous to G638 1014 DNA Glycine max Predicted polypeptide sequence is orthologous to G638 1015 DNA Glycine max Predicted polypeptide sequence is orthologous to G638 1016 DNA Medicago truncatula Predicted polypeptide sequence is orthologous to G638 1017 DNA Glycine max Predicted polypeptide sequence is orthologous to G652 1018 DNA Glycine max Predicted polypeptide sequence is orthologous to G652 1019 DNA Glycine max Predicted polypeptide sequence is orthologous to G652 1020 DNA Glycine max Predicted polypeptide sequence is orthologous to G652 1021 DNA Glycine max Predicted polypeptide sequence is orthologous to G652 1022 DNA Glycine max Predicted polypeptide sequence is orthologous to G652 1023 DNA Glycine max Predicted polypeptide sequence is orthologous to G652 1024 DNA Glycine max Predicted polypeptide sequence is orthologous to G652 1025 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G652 1026 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G652 1027 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G652 1028 DNA Zea mays Predicted polypeptide sequence is orthologous to G652 1029 DNA Zea mays Predicted polypeptide sequence is orthologous to G652 1030 DNA Zea mays Predicted polypeptide sequence is orthologous to G652 1031 DNA Zea mays Predicted polypeptide sequence is orthologous to G652 1032 DNA Zea mays Predicted polypeptide sequence is orthologous to G652 1033 DNA Zea mays Predicted polypeptide sequence is orthologous to G652 1034 DNA Zea mays Predicted polypeptide sequence is orthologous to G652 1035 PRT Oryza sativa Orthologous to G652 1036 PRT Oryza sativa Orthologous to G652 1037 PRT Oryza sativa Orthologous to G652 1038 PRT Oryza sativa Orthologous to G652 1039 PRT Oryza sativa Orthologous to G652 1040 PRT Oryza sativa Orthologous to G652 1041 PRT Oryza sativa Orthologous to G652 1042 PRT Oryza sativa Orthologous to G652 1043 DNA Glycine max Predicted polypeptide sequence is orthologous to G663 1044 DNA Glycine max Predicted polypeptide sequence is orthologous to G664 1045 DNA Glycine max Predicted polypeptide sequence is orthologous to G664 1046 DNA Glycine max Predicted polypeptide sequence is orthologous to G664 1047 DNA Glycine max Predicted polypeptide sequence is orthologous to G664 1048 DNA Glycine max Predicted polypeptide sequence is orthologous to G664 1049 DNA Glycine max Predicted polypeptide sequence is orthologous to G664 1050 DNA Glycine max Predicted polypeptide sequence is orthologous to G664 1051 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G664 1052 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G664 1053 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G664 1054 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G664 1055 DNA Zea mays Predicted polypeptide sequence is orthologous to G664 1056 DNA Zea mays Predicted polypeptide sequence is orthologous to G664 1057 DNA Zea mays Predicted polypeptide sequence is orthologous to G664 1058 DNA Zea mays Predicted polypeptide sequence is orthologous to G664 1059 DNA Zea mays Predicted polypeptide sequence is orthologous to G664 1060 DNA Zea mays Predicted polypeptide sequence is orthologous to G664 1061 DNA Zea mays Predicted polypeptide sequence is orthologous to G664 1062 DNA Zea mays Predicted polypeptide sequence is orthologous to G664 1063 G3509 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G664 1064 G3506 PRT Oryza sativa Orthologous to G664 1065 G3504 PRT Oryza sativa Orthologous to G664 1066 PRT Oryza sativa Orthologous to G664 1067 PRT Oryza sativa Orthologous to G664 1068 G3503 PRT Oryza sativa indica Orthologous to G664 1069 G3505 PRT Oryza sativa japonica Orthologous to G664 1070 G3507 PRT Oryza sativa japonica Orthologous to G664 1071 G3508 PRT Oryza sativa japonica Orthologous to G664 1072 G3509 PRT Lycopersicon Orthologous to G664 esculentum 1073 PRT Hordeum vulgare Orthologous to G664 subsp. vulgare 1074 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G680 1075 DNA Zea mays Predicted polypeptide sequence is orthologous to G680 1076 DNA Glycine max Predicted polypeptide sequence is orthologous to G682 1077 DNA Hordeum vulgare Predicted polypeptide subsp. vulgare sequence is orthologous to G682 1078 DNA Populus tremula x Predicted polypeptide Populus tremuloides sequence is orthologous to G682 1079 DNA Triticum aestivum Predicted polypeptide sequence is orthologous to G682 1080 DNA Gossypium arboreum Predicted polypeptide sequence is orthologous to G682 1081 PRT Oryza sativa Orthologous to G682 1082 PRT Oryza sativa Orthologous to G682 1083 PRT Glycine max Orthologous to G682 1084 PRT Glycine max Orthologous to G682 1085 PRT Glycine max Orthologous to G682 1086 PRT Glycine max Orthologous to G682 1087 PRT Glycine max Orthologous to G682 1088 PRT Glycine max Orthologous to G682 1089 PRT Zea mays Orthologous to G682 1090 PRT Zea mays Orthologous to G682 1091 DNA Glycine max Predicted polypeptide sequence is orthologous to G715, G1646 1092 DNA Glycine max Predicted polypeptide sequence is orthologous to G715, G1646 1093 DNA Glycine max Predicted polypeptide sequence is orthologous to G715, G1646 1094 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G715, G1646 1095 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G715, G1646 1096 DNA Zea mays Predicted polypeptide sequence is orthologous to G715, G1646 1097 DNA Zea mays Predicted polypeptide sequence is orthologous to G715, G1646 1098 DNA Zea mays Predicted polypeptide sequence is orthologous to G715, G1646 1099 DNA Zea mays Predicted polypeptide sequence is orthologous to G715, G1646 1100 DNA Zea mays Predicted polypeptide sequence is orthologous to G715, G1646 1101 DNA Zea mays Predicted polypeptide sequence is orthologous to G715, G1646 1102 DNA Zea mays Predicted polypeptide sequence is orthologous to G715, G1646 1103 DNA Zea mays Predicted polypeptide sequence is orthologous to G715, G1646 1104 DNA Zea mays Predicted polypeptide sequence is orthologous to G715, G1646 1105 PRT Oryza sativa Orthologous to G715, G1646 1106 PRT Oryza sativa Orthologous to G715, G1646 1107 PRT Oryza sativa Orthologous to G715, G1646 1108 PRT Oryza sativa Orthologous to G715, G1646 1109 PRT Oryza sativa Orthologous to G715, G1646 1110 PRT Oryza sativa Orthologous to G715, G1646 1111 DNA Glycine max Predicted polypeptide sequence is orthologous to G720 1112 DNA Glycine max Predicted polypeptide sequence is orthologous to G720 1113 DNA Glycine max Predicted polypeptide sequence is orthologous to G720 1114 DNA Glycine max Predicted polypeptide sequence is orthologous to G720 1115 DNA Medicago truncatula Predicted polypeptide sequence is orthologous to G720 1116 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G720 1117 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G720 1118 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G720 1119 DNA Solanum tuberosum Predicted polypeptide sequence is orthologous to G720 1120 DNA Glycine max Predicted polypeptide sequence is orthologous to G736 1121 DNA Glycine max Predicted polypeptide sequence is orthologous to G736 1122 PRT Oryza sativa Orthologous to G736 1123 DNA Glycine max Predicted polypeptide sequence is orthologous to G748 1124 DNA Glycine max Predicted polypeptide sequence is orthologous to G748 1125 DNA Glycine max Predicted polypeptide sequence is orthologous to G748 1126 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G748 1127 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G748 1128 DNA Zea mays Predicted polypeptide sequence is orthologous to G748 1129 PRT Oryza sativa Orthologous to G748 1130 PRT Oryza sativa Orthologous to G748 1131 PRT Oryza sativa Orthologous to G748 1132 PRT Oryza sativa Orthologous to G748 1133 PRT Cucurbita maxima Orthologous to G748 1134 DNA Glycine max Predicted polypeptide sequence is orthologous to G789, G1494 1135 DNA Glycine max Predicted polypeptide sequence is orthologous to G789, G1494 1136 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G789 1137 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G789, G1494 1138 DNA Zea mays Predicted polypeptide sequence is orthologous to G789, G1494 1139 PRT Oryza sativa Orthologous to G789, G1494 1140 PRT Oryza sativa Orthologous to G789, G1494 1141 PRT Oryza sativa Orthologous to G789, G1494 1142 DNA Glycine max Predicted polypeptide sequence is orthologous to G801 1143 DNA Glycine max Predicted polypeptide sequence is orthologous to G801 1144 DNA Zea mays Predicted polypeptide sequence is orthologous to G801 1145 DNA Glycine max Predicted polypeptide sequence is orthologous to G849 1146 DNA Glycine max Predicted polypeptide sequence is orthologous to G849 1147 DNA Glycine max Predicted polypeptide sequence is orthologous to G849 1148 DNA Glycine max Predicted polypeptide sequence is orthologous to G849 1149 DNA Glycine max Predicted polypeptide sequence is orthologous to G849 1150 DNA Glycine max Predicted polypeptide sequence is orthologous to G849 1151 DNA Zea mays Predicted polypeptide sequence is orthologous to G849 1152 DNA Zea mays Predicted polypeptide sequence is orthologous to G849 1153 DNA Zea mays Predicted polypeptide sequence is orthologous to G849 1154 DNA Glycine max Predicted polypeptide sequence is orthologous to G864 1155 DNA Glycine max Predicted polypeptide sequence is orthologous to G864 1156 DNA Zea mays Predicted polypeptide sequence is orthologous to G864 1157 PRT Oryza sativa Orthologous to G864 1158 PRT Oryza sativa Orthologous to G864 1159 DNA Glycine max Predicted polypeptide sequence is orthologous to G867, G1930 1160 DNA Glycine max Predicted polypeptide sequence is orthologous to G867, G1930 1161 DNA Glycine max Predicted polypeptide sequence is orthologous to G867, G1930 1162 DNA Glycine max Predicted polypeptide sequence is orthologous to G867, G1930 1163 DNA Glycine max Predicted polypeptide sequence is orthologous to G867, G1930 1164 DNA Glycine max Predicted polypeptide sequence is orthologous to G867 1165 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G867 1166 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G867, G1930 1167 DNA Zea mays Predicted polypeptide sequence is orthologous to G867, G1930 1168 DNA Zea mays Predicted polypeptide sequence is orthologous to G867, G1930 1169 DNA Zea mays Predicted polypeptide sequence is orthologous to G867, G1930 1170 DNA Zea mays Predicted polypeptide sequence is orthologous to G867, G1930 1171 DNA Glycine max Predicted polypeptide sequence is orthologous to G867, G1930 1172 DNA Mesembryanthemum Predicted polypeptide crystallinum sequence is orthologous to G867, G1930 1173 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G867, G1930 1174 DNA Solanum tuberosum Predicted polypeptide sequence is orthologous to G867, G1930 1175 DNA Hordeum vulgare Predicted polypeptide sequence is orthologous to G867, G1930 1176 PRT Oryza sativa Orthologous to G867, G1930 1177 PRT Oryza sativa Orthologous to G867, G1930 1178 PRT Oryza sativa Orthologous to G867, G1930 1179 PRT Oryza sativa Orthologous to G867, G1930 1180 PRT Oryza sativa Orthologous to G867, G1930 1181 PRT Oryza sativa Orthologous to G867, G1930 1182 PRT Glycine max Orthologous to G867, G1930 1183 PRT Glycine max Orthologous to G867, G1930 1184 PRT Glycine max Orthologous to G867, G1930 1185 PRT Zea mays Orthologous to G867, G1930 1186 PRT Zea mays Orthologous to G867, G1930 1187 DNA Glycine max Predicted polypeptide sequence is orthologous to G869 1188 DNA Glycine max Predicted polypeptide sequence is orthologous to G869 1189 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G869 1190 DNA Zea mays Predicted polypeptide sequence is orthologous to G869 1191 PRT Oryza sativa Orthologous to G869 1192 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G877 1193 DNA Glycine max Predicted polypeptide sequence is orthologous to G881 1194 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G881 1195 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G881 1196 DNA Zea mays Predicted polypeptide sequence is orthologous to G881 1197 DNA Zea mays Predicted polypeptide sequence is orthologous to G881 1198 DNA Zea mays Predicted polypeptide sequence is orthologous to G881 1199 DNA Zea mays Predicted polypeptide sequence is orthologous to G881 1200 PRT Oryza sativa Orthologous to G881 1201 PRT Oryza sativa Orthologous to G892 1202 DNA Mentha x piperita Predicted polypeptide sequence is orthologous to G896 1203 DNA Glycine max Predicted polypeptide sequence is orthologous to G910 1204 DNA Glycine max Predicted polypeptide sequence is orthologous to G912 1205 DNA Glycine max Predicted polypeptide sequence is orthologous to G912 1206 DNA Glycine max Predicted polypeptide sequence is orthologous to G912 1207 DNA Glycine max Predicted polypeptide sequence is orthologous to G912 1208 DNA Glycine max Predicted polypeptide sequence is orthologous to G912 1209 DNA Glycine max Predicted polypeptide sequence is orthologous to G912 1210 DNA Glycine max Predicted polypeptide sequence is orthologous to G912 1211 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G912 1212 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G912, G913 1213 DNA Zea mays Predicted polypeptide sequence is orthologous to G912 1214 DNA Zea mays Predicted polypeptide sequence is orthologous to G912 1215 DNA Zea mays Predicted polypeptide sequence is orthologous to G912, G913 1216 DNA Zea mays Predicted polypeptide sequence is orthologous to G912 1217 DNA Zea mays Predicted polypeptide sequence is orthologous to G912 1218 DNA Brassica napus Predicted polypeptide sequence is orthologous to G912, G913 1219 DNA Solanum tuberosum Predicted polypeptide sequence is orthologous to G912 1220 DNA Descurainia sophia Predicted polypeptide sequence is orthologous to G912 1221 PRT Oryza sativa Orthologous to G912 1222 PRT Oryza sativa Orthologous to G912, G913 1223 PRT Oryza sativa Orthologous to G912, G913 1224 PRT Oryza sativa Orthologous to G912 1225 PRT Brassica napus Orthologous to G912 1226 PRT Nicotiana tabacum Orthologous to G912 1227 PRT Oryza sativa Orthologous to G912 1228 PRT Oryza sativa Orthologous to G912 1229 PRT Oryza sativa Orthologous to G912 1230 PRT Oryza sativa Orthologous to G912 1231 PRT Oryza sativa Orthologous to G912 1232 PRT Oryza sativa Orthologous to G912 1233 PRT Oryza sativa Orthologous to G912 1234 PRT Oryza sativa Orthologous to G912 1235 PRT Oryza sativa Orthologous to G912 1236 PRT Oryza sativa Orthologous to G912 1237 PRT Glycine max Orthologous to G912 1238 PRT Glycine max Orthologous to G912 1239 PRT Glycine max Orthologous to G912 1240 PRT Glycine max Orthologous to G912 1241 PRT Glycine max Orthologous to G912 1242 PRT Glycine max Orthologous to G912 1243 PRT Glycine max Orthologous to G912 1244 PRT Zea mays Orthologous to G912 1245 PRT Zea mays Orthologous to G912 1246 PRT Zea mays Orthologous to G912 1247 PRT Zea mays Orthologous to G912 1248 PRT Zea mays Orthologous to G912 1249 DNA Glycine max Predicted polypeptide sequence is orthologous to G922 1250 DNA Glycine max Predicted polypeptide sequence is orthologous to G922 1251 DNA Glycine max Predicted polypeptide sequence is orthologous to G922 1252 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G922 1253 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G922 1254 PRT Oryza sativa Orthologous to G922 1255 PRT Oryza sativa Orthologous to G922 1256 PRT Oryza sativa Orthologous to G922 1257 PRT Oryza sativa Orthologous to G922 1258 DNA Glycine max Predicted polypeptide sequence is orthologous to G926 1259 DNA Glycine max Predicted polypeptide sequence is orthologous to G926 1260 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G926 1261 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G926 1262 DNA Zea mays Predicted polypeptide sequence is orthologous to G926 1263 PRT Brassica napus Orthologous to G926 1264 DNA Glycine max Predicted polypeptide sequence is orthologous to G961 1265 DNA Glycine max Predicted polypeptide sequence is orthologous to G961 1266 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G961 1267 DNA Zea mays Predicted polypeptide sequence is orthologous to G961 1268 DNA Zea mays Predicted polypeptide sequence is orthologous to G961 1269 DNA Zea mays Predicted polypeptide sequence is orthologous to G961 1270 PRT Oryza sativa Orthologous to G961 1271 DNA Glycine max Predicted polypeptide sequence is orthologous to G974 1272 DNA Glycine max Predicted polypeptide sequence is orthologous to G974 1273 DNA Glycine max Predicted polypeptide sequence is orthologous to G974 1274 DNA Glycine max Predicted polypeptide sequence is orthologous to G974 1275 DNA Glycine max Predicted polypeptide sequence is orthologous to G974 1276 DNA Glycine max Predicted polypeptide sequence is orthologous to G974 1277 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G974 1278 DNA Zea mays Predicted polypeptide sequence is orthologous to G974 1279 DNA Zea mays Predicted polypeptide sequence is orthologous to G974 1280 DNA Zea mays Predicted polypeptide sequence is orthologous to G974 1281 DNA Zea mays Predicted polypeptide sequence is orthologous to G974 1282 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G974 1283 DNA Glycine max Predicted polypeptide sequence is orthologous to G974 1284 DNA Solanum tuberosum Predicted polypeptide sequence is orthologous to G974 1285 DNA Poplar xylem Predicted polypeptide sequence is orthologous to G974 1286 DNA Medicago truncatula Predicted polypeptide sequence is orthologous to G974 1287 DNA Sorghum bicolor Predicted polypeptide sequence is orthologous to G974 1288 PRT Oryza sativa Orthologous to G974 1289 PRT Oryza sativa Orthologous to G974 1290 PRT Oryza sativa Orthologous to G974 1291 PRT Atriplex hortensis Orthologous to G974 1292 DNA Glycine max Predicted polypeptide sequence is orthologous to G975, G2583 1293 DNA Glycine max Predicted polypeptide sequence is orthologous to G975, G2583 1294 DNA Glycine max Predicted polypeptide sequence is orthologous to G975, G2583 1295 DNA Glycine max Predicted polypeptide sequence is orthologous to G975, G2583 1296 DNA Glycine max Predicted polypeptide sequence is orthologous to G975, G2583 1297 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G975 1298 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G975, G2583 1299 DNA Zea mays Predicted polypeptide sequence is orthologous to G975, G2583 1300 DNA Zea mays Predicted polypeptide sequence is orthologous to G975, G2583 1301 DNA Brassica rapa Predicted polypeptide sequence is orthologous to G975, G2583 1302 PRT Oryza sativa Orthologous to G975, G2583 1303 DNA Glycine max Predicted polypeptide sequence is orthologous to G979 1304 DNA Glycine max Predicted polypeptide sequence is orthologous to G979 1305 DNA Glycine max Predicted polypeptide sequence is orthologous to G979 1306 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G979 1307 DNA Zea mays Predicted polypeptide sequence is orthologous to G979 1308 DNA Zea mays Predicted polypeptide sequence is orthologous to G979 1309 DNA Zea mays Predicted polypeptide sequence is orthologous to G979 1310 PRT Oryza sativa Orthologous to G979 1311 PRT Oryza sativa Orthologous to G979 1312 PRT Oryza sativa Orthologous to G979 1313 PRT Oryza sativa Orthologous to G979 1314 PRT Oryza sativa Orthologous to G979 1315 DNA Glycine max Predicted polypeptide sequence is orthologous to G987 1316 DNA Glycine max Predicted polypeptide sequence is orthologous to G987 1317 DNA Glycine max Predicted polypeptide sequence is orthologous to G987 1318 DNA Glycine max Predicted polypeptide sequence is orthologous to G987 1319 DNA Glycine max Predicted polypeptide sequence is orthologous to G987 1320 DNA Glycine max Predicted polypeptide sequence is orthologous to G987 1321 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G987 1322 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G987 1323 DNA Zea mays Predicted polypeptide sequence is orthologous to G987 1324 PRT Oryza sativa Orthologous to G987 1325 PRT Oryza sativa Orthologous to G988 1326 PRT Oryza sativa Orthologous to G988 1327 PRT Capsella rubella Orthologous to G988 1328 DNA Glycine max Predicted polypeptide sequence is orthologous to G1040 1329 DNA Glycine max Predicted polypeptide sequence is orthologous to G1040 1330 DNA Glycine max Predicted polypeptide sequence is orthologous to G1040 1331 DNA Glycine max Predicted polypeptide sequence is orthologous to G1040 1332 DNA Glycine max Predicted polypeptide sequence is orthologous to G1040 1333 DNA Zea mays Predicted polypeptide sequence is orthologous to G1040 1334 DNA Zea mays Predicted polypeptide sequence is orthologous to G1040 1335 DNA Zea mays Predicted polypeptide sequence is orthologous to G1040 1336 DNA Zea mays Predicted polypeptide sequence is orthologous to G1040 1337 DNA Zea mays Predicted polypeptide sequence is orthologous to G1040 1338 PRT Oryza sativa Orthologous to G1040 1339 PRT Oryza sativa Orthologous to G1040 1340 DNA Glycine max Predicted polypeptide sequence is orthologous to G1047 1341 DNA Zea mays Predicted polypeptide sequence is orthologous to G1047 1342 PRT Oryza sativa Orthologous to G1047 1343 PRT Oryza sativa Orthologous to G1047 1344 DNA Glycine max Predicted polypeptide sequence is orthologous to G1051, G1052 1345 DNA Glycine max Predicted polypeptide sequence is orthologous to G1051, G1052 1346 DNA Glycine max Predicted polypeptide sequence is orthologous to G1051, G1052 1347 DNA Glycine max Predicted polypeptide sequence is orthologous to G1051, G1052 1348 DNA Glycine max Predicted polypeptide sequence is orthologous to G1051, G1052 1349 DNA Glycine max Predicted polypeptide sequence is orthologous to G1051, G1052 1350 DNA Glycine max Predicted polypeptide sequence is orthologous to G1051, G1052 1351 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1051, G1052 1352 DNA Zea mays Predicted polypeptide sequence is orthologous to G1051, G1052 1353 DNA Zea mays Predicted polypeptide sequence is orthologous to G1051, G1052 1354 DNA Zea mays Predicted polypeptide sequence is orthologous to G1051, G1052 1355 DNA Zea mays Predicted polypeptide sequence is orthologous to G1051, G1052 1356 DNA Zea mays Predicted polypeptide sequence is orthologous to G1051, G1052 1357 DNA Zea mays Predicted polypeptide sequence is orthologous to G1051, G1052 1358 DNA Zea mays Predicted polypeptide sequence is orthologous to G1051, G1052 1359 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1052 1360 DNA Zea mays Predicted polypeptide sequence is orthologous to G1052 1361 DNA Zea mays Predicted polypeptide sequence is orthologous to G1052 1362 PRT Oryza sativa Orthologous to G1051, G1052 1363 PRT Oryza sativa Orthologous to G1051, G1052 1364 PRT Oryza sativa Orthologous to G1051, G1052 1365 DNA Glycine max Predicted polypeptide sequence is orthologous to G1062 1366 DNA Glycine max Predicted polypeptide sequence is orthologous to G1062 1367 DNA Glycine max Predicted polypeptide sequence is orthologous to G1062 1368 DNA Glycine max Predicted polypeptide sequence is orthologous to G1062 1369 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1062 1370 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1062 1371 DNA Zea mays Predicted polypeptide sequence is orthologous to G1062 1372 DNA Zea mays Predicted polypeptide sequence is orthologous to G1062 1373 DNA Zea mays Predicted polypeptide sequence is orthologous to G1062 1374 DNA Zea mays Predicted polypeptide sequence is orthologous to G1062 1375 DNA Zea mays Predicted polypeptide sequence is orthologous to G1062 1376 DNA Medicago truncatula Predicted polypeptide sequence is orthologous to G1062 1377 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G1062 1378 PRT Oryza sativa Orthologous to G1062 1379 DNA Glycine max Predicted polypeptide sequence is orthologous to G1063, G2143 1380 DNA Glycine max Predicted polypeptide sequence is orthologous to G1063, G2143 1381 DNA Glycine max Predicted polypeptide sequence is orthologous to G1063, G2143 1382 DNA Glycine max Predicted polypeptide sequence is orthologous to G1063, G2143 1383 DNA Glycine max Predicted polypeptide sequence is orthologous to G1063, G2143 1384 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G1063, G2143 1385 DNA Glycine max Predicted polypeptide sequence is orthologous to G1064 1386 DNA Glycine max Predicted polypeptide sequence is orthologous to G1064 1387 DNA Glycine max Predicted polypeptide sequence is orthologous to G1064 1388 DNA Zea mays Predicted polypeptide sequence is orthologous to G1064 1389 DNA Zea mays Predicted polypeptide sequence is orthologous to G1064 1390 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G1064 1391 PRT Oryza sativa Orthologous to G1064 1392 PRT Gossypium hirsutum Orthologous to G1064 1393 DNA Glycine max Predicted polypeptide sequence is orthologous to G1069 1394 DNA Glycine max Predicted polypeptide sequence is orthologous to G1069 1395 PRT Oryza sativa Orthologous to G1069, G1073 1396 DNA Zea mays Predicted polypeptide sequence is orthologous to G1069 1397 DNA Lotus japonicus Predicted polypeptide sequence is orthologous to G1069 1398 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G1073 1399 PRT Oryza sativa Orthologous to G1073 1400 PRT Oryza sativa Orthologous to G1073 1401 PRT Oryza sativa Orthologous to G1073 1402 PRT Oryza sativa Orthologous to G1073 1403 PRT Oryza sativa Orthologous to G1073 1404 PRT Oryza sativa Orthologous to G1073 1405 PRT Oryza sativa Orthologous to G1073 1406 PRT Oryza sativa Orthologous to G1073 1407 PRT Oryza sativa Orthologous to G1073 1408 PRT Oryza sativa Orthologous to G1073 1409 PRT Oryza sativa Orthologous to G1073 1410 PRT Oryza sativa Orthologous to G1073 1411 PRT Glycine max Orthologous to G1073 1412 PRT Glycine max Orthologous to G1073 1413 PRT Glycine max Orthologous to G1073 1414 PRT Glycine max Orthologous to G1073 1415 PRT Glycine max Orthologous to G1073 1416 PRT Glycine max Orthologous to G1073 1417 PRT Glycine max Orthologous to G1073 1418 PRT Zea mays Orthologous to G1073 1419 DNA Glycine max Predicted polypeptide sequence is orthologous to G1075 1420 DNA Glycine max Predicted polypeptide sequence is orthologous to G1075 1421 DNA Glycine max Predicted polypeptide sequence is orthologous to G1075 1422 DNA Glycine max Predicted polypeptide sequence is orthologous to G1075 1423 DNA Glycine max Predicted polypeptide sequence is orthologous to G1075 1424 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1075 1425 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1075 1426 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1075 1427 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1089 1428 DNA Zea mays Predicted polypeptide sequence is orthologous to G1089 1429 DNA Zea mays Predicted polypeptide sequence is orthologous to G1089 1430 DNA Zea mays Predicted polypeptide sequence is orthologous to G1089 1431 DNA Zea mays Predicted polypeptide sequence is orthologous to G1089 1432 DNA Zea mays Predicted polypeptide sequence is orthologous to G1089 1433 PRT Oryza sativa Orthologous to G1089 1434 DNA Glycine max Predicted polypeptide sequence is orthologous to G1134, G2555 1435 DNA Glycine max Predicted polypeptide sequence is orthologous to G1134, G2555 1436 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1134, G2555 1437 DNA Glycine max Predicted polypeptide sequence is orthologous to G1140 1438 DNA Glycine max Predicted polypeptide sequence is orthologous to G1140 1439 DNA Glycine max Predicted polypeptide sequence is orthologous to G1140 1440 DNA Glycine max Predicted polypeptide sequence is orthologous to G1140 1441 DNA Glycine max Predicted polypeptide sequence is orthologous to G1140 1442 DNA Glycine max Predicted polypeptide sequence is orthologous to G1140 1443 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1140 1444 DNA Zea mays Predicted polypeptide sequence is orthologous to G1140 1445 DNA Zea mays Predicted polypeptide sequence is orthologous to G1140 1446 DNA Zea mays Predicted polypeptide sequence is orthologous to G1140 1447 DNA Zea mays Predicted polypeptide sequence is orthologous to G1140 1448 DNA Zea mays Predicted polypeptide sequence is orthologous to G1140 1449 DNA Zea mays Predicted polypeptide sequence is orthologous to G1140 1450 DNA Zea mays Predicted polypeptide sequence is orthologous to G1140 1451 DNA Zea mays Predicted polypeptide sequence is orthologous to G1140 1452 DNA Zea mays Predicted polypeptide sequence is orthologous to G1140 1453 PRT Oryza sativa Orthologous to G1140 1454 PRT Ipomoea batatas Orthologous to G1140 1455 DNA Zea mays Predicted polypeptide sequence is orthologous to G1146 1456 DNA Zea mays Predicted polypeptide sequence is orthologous to G1146 1457 PRT Oryza sativa Orthologous to G1146 1458 PRT Oryza sativa Orthologous to G1146 1459 PRT Oryza sativa Orthologous to G1146 1460 DNA Glycine max Predicted polypeptide sequence is orthologous to G1196 1461 DNA Glycine max Predicted polypeptide sequence is orthologous to G1196 1462 DNA Glycine max Predicted polypeptide sequence is orthologous to G1196 1463 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1196 1464 DNA Zea mays Predicted polypeptide sequence is orthologous to G1196 1465 DNA Zea mays Predicted polypeptide sequence is orthologous to G1196 1466 PRT Oryza sativa Orthologous to G1196 1467 PRT Oryza sativa Orthologous to G1196 1468 DNA Glycine max Predicted polypeptide sequence is orthologous to G1198 1469 DNA Glycine max Predicted polypeptide sequence is orthologous to G1198 1470 DNA Glycine max Predicted polypeptide sequence is orthologous to G1198 1471 DNA Glycine max Predicted polypeptide sequence is orthologous to G1198 1472 DNA Glycine max Predicted polypeptide sequence is orthologous to G1198 1473 DNA Glycine max Predicted polypeptide sequence is orthologous to G1198 1474 DNA Glycine max Predicted polypeptide sequence is orthologous to G1198 1475 DNA Glycine max Predicted polypeptide sequence is orthologous to G1198 1476 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1198 1477 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1198 1478 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1198 1479 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1198 1480 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1198 1481 DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1482 DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1483 DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1484 DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1485 DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1486 DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1487 DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1488 DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1489 DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1490 DNA Zea mays Predicted polypeptide sequence is orthologous to G1198 1491 DNA Nicotiana tabacum Predicted polypeptide sequence is orthologous to G1198 1492 PRT Oryza sativa Orthologous to G1198 1493 PRT Oryza sativa Orthologous to G1198 1494 PRT Oryza sativa Orthologous to G1198 1495 PRT Oryza sativa Orthologous to G1198 1496 PRT Oryza sativa Orthologous to G1198 1497 PRT Oryza sativa Orthologous to G1198 1498 PRT Oryza sativa Orthologous to G1198 1499 DNA Zea mays Predicted polypeptide sequence is orthologous to G1225 1500 PRT Oryza sativa Orthologous to G1225 1501 PRT Oryza sativa Orthologous to G1226 1502 DNA Glycine max Predicted polypeptide sequence is orthologous to G1229 1503 PRT Oryza sativa Orthologous to G1229 1504 PRT Oryza sativa Orthologous to G1229 1505 DNA Glycine max Predicted polypeptide sequence is orthologous to G1255 1506 DNA Glycine max Predicted polypeptide sequence is orthologous to G1255 1507 DNA Glycine max Predicted polypeptide sequence is orthologous to G1255 1508 DNA Glycine max Predicted polypeptide sequence is orthologous to G1255 1509 DNA Glycine max Predicted polypeptide sequence is orthologous to G1255 1510 DNA Glycine max Predicted polypeptide sequence is orthologous to G1255 1511 DNA Glycine max Predicted polypeptide sequence is orthologous to G1255 1512 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1255 1513 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1255 1514 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1255 1515 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1255 1516 DNA Zea mays Predicted polypeptide sequence is orthologous to G1255 1517 DNA Zea mays Predicted polypeptide sequence is orthologous to G1255 1518 DNA Zea mays Predicted polypeptide sequence is orthologous to G1255 1519 DNA Zea mays Predicted polypeptide sequence is orthologous to G1255 1520 DNA Zea mays Predicted polypeptide sequence is orthologous to G1255 1521 DNA Zea mays Predicted polypeptide sequence is orthologous to G1255 1522 PRT Oryza sativa Orthologous to G1255 1523 DNA Glycine max Predicted polypeptide sequence is orthologous to G1266 1524 DNA Glycine max Predicted polypeptide sequence is orthologous to G1266 1525 DNA Glycine max Predicted polypeptide sequence is orthologous to G1266 1526 DNA Glycine max Predicted polypeptide sequence is orthologous to G1266 1527 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1266 1528 PRT Nicotiana tabacum Orthologous to G1266 1529 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1275 1530 DNA Zea mays Predicted polypeptide sequence is orthologous to G1275 1531 DNA Zea mays Predicted polypeptide sequence is orthologous to G1275 1532 DNA Zea mays Predicted polypeptide sequence is orthologous to G1275 1533 PRT Oryza sativa Orthologous to G1275 1534 PRT Oryza sativa Orthologous to G1275 1535 PRT Oryza sativa Orthologous to G1275 1536 DNA Glycine max Predicted polypeptide sequence is orthologous to G1322 1537 DNA Glycine max Predicted polypeptide sequence is orthologous to G1322 1538 DNA Glycine max Predicted polypeptide sequence is orthologous to G1322 1539 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1322 1540 PRT Oryza sativa Orthologous to G1322 1541 PRT Oryza sativa Orthologous to G1322 1542 DNA Zea mays Predicted polypeptide sequence is orthologous to G1323 1543 DNA Zea mays Predicted polypeptide sequence is orthologous to G1323 1544 DNA Glycine max Predicted polypeptide sequence is orthologous to G1330 1545 DNA Glycine max Predicted polypeptide sequence is orthologous to G1330 1546 DNA Glycine max Predicted polypeptide sequence is orthologous to G1330 1547 DNA Glycine max Predicted polypeptide sequence is orthologous to G1330 1548 DNA Glycine max Predicted polypeptide sequence is orthologous to G1330 1549 DNA Glycine max Predicted polypeptide sequence is orthologous to G1330 1550 DNA Glycine max Predicted polypeptide sequence is orthologous to G1330 1551 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1330 1552 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1330 1553 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1330 1554 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1330 1555 DNA Zea mays Predicted polypeptide sequence is orthologous to G1330 1556 DNA Zea mays Predicted polypeptide sequence is orthologous to G1330 1557 DNA Zea mays Predicted polypeptide sequence is orthologous to G1330 1558 DNA Zea mays Predicted polypeptide sequence is orthologous to G1330 1559 DNA Zea mays Predicted polypeptide sequence is orthologous to G1330 1560 DNA Zea mays Predicted polypeptide sequence is orthologous to G1330 1561 DNA Zea mays Predicted polypeptide sequence is orthologous to G1330 1562 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G1330 1563 PRT Oryza sativa Orthologous to G1330 1564 PRT Oryza sativa Orthologous to G1330 1565 PRT Oryza sativa Orthologous to G1330 1566 PRT Oryza sativa Orthologous to G1330 1567 DNA Glycine max Predicted polypeptide sequence is orthologous to G1331 1568 DNA Glycine max Predicted polypeptide sequence is orthologous to G1331 1569 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1331 1570 DNA Zea mays Predicted polypeptide sequence is orthologous to G1331 1571 DNA Zea mays Predicted polypeptide sequence is orthologous to G1331 1572 PRT Oryza sativa Orthologous to G1331 1573 DNA Glycine max Predicted polypeptide sequence is orthologous to G1363 1574 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1363 1575 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1363 1576 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1363 1577 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1363 1578 DNA Zea mays Predicted polypeptide sequence is orthologous to G1363 1579 DNA Zea mays Predicted polypeptide sequence is orthologous to G1363 1580 DNA Zea mays Predicted polypeptide sequence is orthologous to G1363 1581 DNA Zea mays Predicted polypeptide sequence is orthologous to G1363 1582 DNA Zea mays Predicted polypeptide sequence is orthologous to G1363 1583 PRT Oryza sativa Orthologous to G1363 1584 PRT Oryza sativa Orthologous to G1363 1585 PRT Oryza sativa Orthologous to G1363 1586 PRT Oryza sativa Orthologous to G1363 1587 DNA Glycine max Predicted polypeptide sequence is orthologous to G1411, G2509 1588 DNA Glycine max Predicted polypeptide sequence is orthologous to G1411, G2509 1589 DNA Glycine max Predicted polypeptide sequence is orthologous to G1411, G2509 1590 DNA Glycine max Predicted polypeptide sequence is orthologous to G1411, G2509 1591 DNA Zea mays Predicted polypeptide sequence is orthologous to G1411, G2509 1592 DNA Glycine max Predicted polypeptide sequence is orthologous to G1417 1593 PRT Oryza sativa Orthologous to G1417 1594 PRT Oryza sativa Orthologous to G1417 1595 DNA Glycine max Predicted polypeptide sequence is orthologous to G1419 1596 DNA Glycine max Predicted polypeptide sequence is orthologous to G1449 1597 DNA Glycine max Predicted polypeptide sequence is orthologous to G1449 1598 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1449 1599 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1449 1600 DNA Zea mays Predicted polypeptide sequence is orthologous to G1449 1601 DNA Zea mays Predicted polypeptide sequence is orthologous to G1449 1602 DNA Zea mays Predicted polypeptide sequence is orthologous to G1449 1603 DNA Zea mays Predicted polypeptide sequence is orthologous to G1449 1604 DNA Glycine max Predicted polypeptide sequence is orthologous to G1451 1605 DNA Glycine max Predicted polypeptide sequence is orthologous to G1451 1606 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1451 1607 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1451 1608 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1451 1609 DNA Zea mays Predicted polypeptide sequence is orthologous to G1451 1610 DNA Zea mays Predicted polypeptide sequence is orthologous to G1451 1611 DNA Zea mays Predicted polypeptide sequence is orthologous to G1451 1612 DNA Zea mays Predicted polypeptide sequence is orthologous to G1451 1613 DNA Medicago Predicted polypeptide truncatula sequence is orthologous to G1451 1614 DNA Solanum Predicted polypeptide tuberosum sequence is orthologous to G1451 1615 DNA Zea mays Predicted polypeptide sequence is orthologous to G1451 1616 DNA Sorghum Predicted polypeptide propinquum sequence is orthologous to G1451 1617 DNA Glycine max Predicted polypeptide sequence is orthologous to G1451 1618 DNA Sorghum bicolor Predicted polypeptide sequence is orthologous to G1451 1619 DNA Hordeum vulgare Predicted polypeptide sequence is orthologous to G1451 1620 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G1451 1621 PRT Oryza sativa Orthologous to G1451 1622 PRT Oryza sativa Orthologous to G1451 1623 PRT Oryza sativa Orthologous to G1451 1624 PRT Oryza sativa Orthologous to G1451 1625 DNA Glycine max Predicted polypeptide sequence is orthologous to G1452 1626 DNA Glycine max Predicted polypeptide sequence is orthologous to G1478 1627 DNA Glycine max Predicted polypeptide sequence is orthologous to G1478 1628 DNA Glycine max Predicted polypeptide sequence is orthologous to G1478 1629 DNA Zea mays Predicted polypeptide sequence is orthologous to G1478 1630 DNA Glycine max Predicted polypeptide sequence is orthologous to G1482 1631 DNA Glycine max Predicted polypeptide sequence is orthologous to G1482 1632 DNA Glycine max Predicted polypeptide sequence is orthologous to G1482 1633 DNA Glycine max Predicted polypeptide sequence is orthologous to G1482 1634 DNA Glycine max Predicted polypeptide sequence is orthologous to G1482 1635 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1482 1636 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1482 1637 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1482 1638 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1482 1639 DNA Zea mays Predicted polypeptide sequence is orthologous to G1482 1640 DNA Zea mays Predicted polypeptide sequence is orthologous to G1482 1641 DNA Zea mays Predicted polypeptide sequence is orthologous to G1482 1642 DNA Zea mays Predicted polypeptide sequence is orthologous to G1482 1643 DNA Zea mays Predicted polypeptide sequence is orthologous to G1482 1644 DNA Zea mays Predicted polypeptide sequence is orthologous to G1482 1645 PRT Oryza sativa Orthologous to G1482 1646 PRT Oryza sativa Orthologous to G1482 1647 DNA Glycine max Predicted polypeptide sequence is orthologous to G1488 1648 DNA Glycine max Predicted polypeptide sequence is orthologous to G1488 1649 DNA Glycine max Predicted polypeptide sequence is orthologous to G1488 1650 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1488 1651 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1488 1652 DNA Zea mays Predicted polypeptide sequence is orthologous to G1488 1653 DNA Zea mays Predicted polypeptide sequence is orthologous to G1488 1654 DNA Zea mays Predicted polypeptide sequence is orthologous to G1488 1655 PRT Oryza sativa Orthologous to G1488 1656 PRT Oryza sativa Orthologous to G1488 1657 PRT Oryza sativa Orthologous to G1488 1658 PRT Oryza sativa Orthologous to G1499 1659 DNA Brassica rapa subsp. Predicted polypeptide pekinensis sequence is orthologous to G1499 1660 DNA Glycine max Predicted polypeptide sequence is orthologous to G1519 1661 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1519 1662 DNA Zea mays Predicted polypeptide sequence is orthologous to G1519 1663 DNA Zea mays Predicted polypeptide sequence is orthologous to G1519 1664 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G1519 1665 DNA Glycine max Predicted polypeptide sequence is orthologous to G1526 1666 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1526 1667 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1526 1668 DNA Zea mays Predicted polypeptide sequence is orthologous to G1526 1669 DNA Glycine max Predicted polypeptide sequence is orthologous to G1540 1670 PRT Oryza sativa Orthologous to G1540 1671 DNA Glycine max Predicted polypeptide sequence is orthologous to G1543 1672 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1543 1673 DNA Zea mays Predicted polypeptide sequence is orthologous to G1543 1674 PRT Oryza sativa Orthologous to G1543 1675 DNA Zea mays Predicted polypeptide sequence is orthologous to G1637 1676 DNA Zea mays Predicted polypeptide sequence is orthologous to G1637 1677 DNA Zea mays Predicted polypeptide sequence is orthologous to G1637 1678 DNA Glycine max Predicted polypeptide sequence is orthologous to G1640 1679 DNA Glycine max Predicted polypeptide sequence is orthologous to G1640 1680 DNA Glycine max Predicted polypeptide sequence is orthologous to G1640 1681 PRT Oryza sativa Orthologous to G1640 1682 DNA Zea mays Predicted polypeptide sequence is orthologous to G1645 1683 DNA Zea mays Predicted polypeptide sequence is orthologous to G1645 1684 DNA Zea mays Predicted polypeptide sequence is orthologous to G1645 1685 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G1645 1686 DNA Medicago truncatula Predicted polypeptide sequence is orthologous to G1645 1687 PRT Oryza sativa Orthologous to G1645 1688 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1646 1689 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1646 1690 DNA Glycine max Predicted polypeptide sequence is orthologous to G1652 1691 DNA Glycine max Predicted polypeptide sequence is orthologous to G1652 1692 DNA Glycine max Predicted polypeptide sequence is orthologous to G1652 1693 DNA Glycine max Predicted polypeptide sequence is orthologous to G1652 1694 DNA Glycine max Predicted polypeptide sequence is orthologous to G1652 1695 DNA Glycine max Predicted polypeptide sequence is orthologous to G1652 1696 DNA Glycine max Predicted polypeptide sequence is orthologous to G1652 1697 DNA Glycine max Predicted polypeptide sequence is orthologous to G1652 1698 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1652 1699 DNA Zea mays Predicted polypeptide sequence is orthologous to G1652 1700 DNA Zea mays Predicted polypeptide sequence is orthologous to G1652 1701 PRT Oryza sativa Orthologous to G1652 1702 PRT Oryza sativa Orthologous to G1652 1703 PRT Oryza sativa Orthologous to G1652 1704 PRT Oryza sativa Orthologous to G1652 1705 PRT Oryza sativa Orthologous to G1652 1706 DNA Glycine max Predicted polypeptide sequence is orthologous to G1672 1707 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1672 1708 DNA Zea mays Predicted polypeptide sequence is orthologous to G1672 1709 DNA Zea mays Predicted polypeptide sequence is orthologous to G1672 1710 PRT Oryza sativa Orthologous to G1672 1711 PRT Oryza sativa Orthologous to G1672 1712 PRT Oryza sativa Orthologous to G1672 1713 PRT Oryza sativa Orthologous to G1672 1714 DNA Glycine max Predicted polypeptide sequence is orthologous to G1750 1715 DNA Glycine max Predicted polypeptide sequence is orthologous to G1750 1716 DNA Glycine max Predicted polypeptide sequence is orthologous to G1750 1717 DNA Glycine max Predicted polypeptide sequence is orthologous to G1750 1718 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1750 1719 DNA Zea mays Predicted polypeptide sequence is orthologous to G1750 1720 DNA Zea mays Predicted polypeptide sequence is orthologous to G1750 1721 DNA Glycine max Predicted polypeptide sequence is orthologous to G1756 1722 DNA Medicago truncatula Predicted polypeptide sequence is orthologous to G1765 1723 DNA Glycine max Predicted polypeptide sequence is orthologous to G1777 1724 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1777 1725 DNA Zea mays Predicted polypeptide sequence is orthologous to G1777 1726 DNA Zea mays Predicted polypeptide sequence is orthologous to G1777 1727 PRT Oryza sativa Orthologous to G1777 1728 DNA Glycine max Predicted polypeptide sequence is orthologous to G1792 1729 DNA Glycine max Predicted polypeptide sequence is orthologous to G1792 1730 DNA Glycine max Predicted polypeptide sequence is orthologous to G1792 1731 DNA Glycine max Predicted polypeptide sequence is orthologous to G1792 1732 DNA Glycine max Predicted polypeptide sequence is orthologous to G1792 1733 DNA Zea mays Predicted polypeptide sequence is orthologous to G1792 1734 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G1792 1735 G3380 PRT Oryza sativa Orthologous to G1792 1736 G3381 PRT Oryza sativa indica Orthologous to G1792 1737 G3383 PRT Oryza sativa japonica Orthologous to G1792 1738 DNA Glycine max Predicted polypeptide sequence is orthologous to G1793 1739 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1793 1740 DNA Zea mays Predicted polypeptide sequence is orthologous to G1793 1741 DNA Zea mays Predicted polypeptide sequence is orthologous to G1793 1742 DNA Zea mays Predicted polypeptide sequence is orthologous to G1793 1743 PRT Oryza sativa Orthologous to G1793 1744 DNA Glycine max Predicted polypeptide sequence is orthologous to G1794 1745 DNA Glycine max Predicted polypeptide sequence is orthologous to G1794 1746 DNA Glycine max Predicted polypeptide sequence is orthologous to G1794 1747 DNA Glycine max Predicted polypeptide sequence is orthologous to G1794 1748 DNA Glycine max Predicted polypeptide sequence is orthologous to G1794 1749 DNA Glycine max Predicted polypeptide sequence is orthologous to G1794 1750 DNA Glycine max Predicted polypeptide sequence is orthologous to G1794 1751 DNA Zea mays Predicted polypeptide sequence is orthologous to G1794 1752 DNA Zea mays Predicted polypeptide sequence is orthologous to G1794 1753 DNA Zea mays Predicted polypeptide sequence is orthologous to G1794 1754 PRT Oryza sativa Orthologous to G1794 1755 PRT Oryza sativa Orthologous to G1794 1756 PRT Oryza sativa Orthologous to G1794 1757 DNA Glycine max Predicted polypeptide sequence is orthologous to G1804 1758 DNA Glycine max Predicted polypeptide sequence is orthologous to G1804 1759 DNA Glycine max Predicted polypeptide sequence is orthologous to G1804 1760 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1804 1761 PRT Oryza sativa Orthologous to G1804 1762 PRT Helianthus annuus Orthologous to G1804 1763 DNA Glycine max Predicted polypeptide sequence is orthologous to G1838 1764 DNA Glycine max Predicted polypeptide sequence is orthologous to G1838 1765 PRT Oryza sativa Orthologous to G1838 1766 DNA Glycine max Predicted polypeptide sequence is orthologous to G1841 1767 DNA Glycine max Predicted polypeptide sequence is orthologous to G1841 1768 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1841 1769 PRT Oryza sativa Orthologous to G1841 1770 DNA Solanum tuberosum Predicted polypeptide sequence is orthologous to G1852 1771 DNA Gossypium arboreum Predicted polypeptide sequence is orthologous to G1852 1772 DNA Medicago truncatula Predicted polypeptide sequence is orthologous to G1852 1773 DNA Glycine max Predicted polypeptide sequence is orthologous to G1852 1774 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G1852 1775 DNA Pinus taeda Predicted polypeptide sequence is orthologous to G1852 1776 DNA Lotus japonicus Predicted polypeptide sequence is orthologous to G1852 1777 DNA Gossypium hirsutum Predicted polypeptide sequence is orthologous to G1852 1778 DNA Solanum tuberosum Predicted polypeptide sequence is orthologous to G1863 1779 DNA Medicago truncatula Predicted polypeptide sequence is orthologous to G1863 1780 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G1863 1781 PRT Oryza sativa Orthologous to G1863 1782 DNA Glycine max Predicted polypeptide sequence is orthologous to G1880 1783 DNA Glycine max Predicted polypeptide sequence is orthologous to G1880 1784 DNA Medicago truncatula Predicted polypeptide sequence is orthologous to G1880 1785 PRT Oryza sativa Orthologous to G1880 1786 DNA Glycine max Predicted polypeptide sequence is orthologous to G1902 1787 DNA Glycine max Predicted polypeptide sequence is orthologous to G1902 1788 DNA Glycine max Predicted polypeptide sequence is orthologous to G1902 1789 DNA Zea mays Predicted polypeptide sequence is orthologous to G1902 1790 PRT Oryza sativa Orthologous to G1902 1791 DNA Glycine max Predicted polypeptide sequence is orthologous to G1927 1792 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1927 1793 DNA Zea mays Predicted polypeptide sequence is orthologous to G1927 1794 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G1927 1795 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1930 1796 DNA Glycine max Predicted polypeptide sequence is orthologous to G1944 1797 DNA Glycine max Predicted polypeptide sequence is orthologous to G1944 1798 DNA Zea mays Predicted polypeptide sequence is orthologous to G1944 1799 DNA Glycine max Predicted polypeptide sequence is orthologous to G1944 1800 DNA Glycine max Predicted polypeptide sequence is orthologous to G1944 1801 DNA Glycine max Predicted polypeptide sequence is orthologous to G1946 1802 DNA Glycine max Predicted polypeptide sequence is orthologous to G1946 1803 DNA Zea mays Predicted polypeptide sequence is orthologous to G1946 1804 DNA Zea mays Predicted polypeptide sequence is orthologous to G1946 1805 PRT Oryza sativa Orthologous to G1946 1806 DNA Glycine max Predicted polypeptide sequence is orthologous to G1948 1807 DNA Glycine max Predicted polypeptide sequence is orthologous to G1948 1808 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1948 1809 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1948 1810 DNA Zea mays Predicted polypeptide sequence is orthologous to G1948 1811 DNA Zea mays Predicted polypeptide sequence is orthologous to G1948 1812 DNA Zea mays Predicted polypeptide sequence is orthologous to G1948 1813 PRT Oryza sativa Orthologous to G1948 1814 DNA Glycine max Predicted polypeptide sequence is orthologous to G1950 1815 DNA Glycine max Predicted polypeptide sequence is orthologous to G1950 1816 DNA Glycine max Predicted polypeptide sequence is orthologous to G1950 1817 DNA Glycine max Predicted polypeptide sequence is orthologous to G1950 1818 DNA Glycine max Predicted polypeptide sequence is orthologous to G1950 1819 DNA Glycine max Predicted polypeptide sequence is orthologous to G1950 1820 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1950 1821 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1950 1822 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1950 1823 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1950 1824 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1950 1825 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1950 1826 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1950 1827 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1950 1828 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1950 1829 DNA Zea mays Predicted polypeptide sequence is orthologous to G1950 1830 DNA Zea mays Predicted polypeptide sequence is orthologous to G1950 1831 DNA Zea mays Predicted polypeptide sequence is orthologous to G1950 1832 DNA Zea mays Predicted polypeptide sequence is orthologous to G1950 1833 DNA Zea mays Predicted polypeptide sequence is orthologous to G1950 1834 DNA Zea mays Predicted polypeptide sequence is orthologous to G1950 1835 DNA Zea mays Predicted polypeptide sequence is orthologous to G1950 1836 DNA Zea mays Predicted polypeptide sequence is orthologous to G1950 1837 DNA Zea mays Predicted polypeptide sequence is orthologous to G1950 1838 PRT Oryza sativa Orthologous to G1950 1839 PRT Oryza sativa Orthologous to G1950 1840 PRT Oryza sativa Orthologous to G1950 1841 PRT Oryza sativa Orthologous to G1950 1842 PRT Oryza sativa Orthologous to G1950 1843 PRT Oryza sativa Orthologous to G1950 1844 PRT Oryza sativa Orthologous to G1950 1845 PRT Oryza sativa Orthologous to G1950 1846 PRT Oryza sativa Orthologous to G1950 1847 DNA Glycine max Predicted polypeptide sequence is orthologous to G1958 1848 DNA Glycine max Predicted polypeptide sequence is orthologous to G1958 1849 DNA Glycine max Predicted polypeptide sequence is orthologous to G1958 1850 DNA Glycine max Predicted polypeptide sequence is orthologous to G1958 1851 DNA Glycine max Predicted polypeptide sequence is orthologous to G1958 1852 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1958 1853 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G1958 1854 DNA Zea mays Predicted polypeptide sequence is orthologous to G1958 1855 DNA Zea mays Predicted polypeptide sequence is orthologous to G1958 1856 DNA Zea mays Predicted polypeptide sequence is orthologous to G1958 1857 PRT Nicotiana tabacum Orthologous to G1958 1858 DNA Glycine max Predicted polypeptide sequence is orthologous to G2007 1859 DNA Glycine max Predicted polypeptide sequence is orthologous to G2007 1860 DNA Zea mays Predicted polypeptide sequence is orthologous to G2007 1861 DNA Zea mays Predicted polypeptide sequence is orthologous to G2007 1862 DNA Zea mays Predicted polypeptide sequence is orthologous to G2007 1863 PRT Oryza sativa Orthologous to G2007 1864 DNA Glycine max Predicted polypeptide sequence is orthologous to G2010, G2347 1865 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G2010, G2347 1866 DNA Zea mays Predicted polypeptide sequence is orthologous to G2010 1867 DNA Zea mays Predicted polypeptide sequence is orthologous to G2010, G2347 1868 DNA Glycine max Predicted polypeptide sequence is orthologous to G2059 1869 DNA Glycine max Predicted polypeptide sequence is orthologous to G2085 1870 DNA Glycine max Predicted polypeptide sequence is orthologous to G2085 1871 DNA Glycine max Predicted polypeptide sequence is orthologous to G2085 1872 DNA Glycine max Predicted polypeptide sequence is orthologous to G2085 1873 DNA Zea mays Predicted polypeptide sequence is orthologous to G2085 1874 PRT Oryza sativa Orthologous to G2085 1875 PRT Oryza sativa Orthologous to G2105 1876 DNA Glycine max Predicted polypeptide sequence is orthologous to G2110 1877 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G2114 1878 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G2114 1879 DNA Zea mays Predicted polypeptide sequence is orthologous to G2114 1880 DNA Zea mays Predicted polypeptide sequence is orthologous to G2114 1881 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G2117 1882 DNA Medicago truncatula Predicted polypeptide sequence is orthologous to G2130 1883 PRT Oryza sativa Orthologous to G2130 1884 PRT Oryza sativa Orthologous to G2130 1885 DNA Glycine max Predicted polypeptide sequence is orthologous to G2140 1886 DNA Glycine max Predicted polypeptide sequence is orthologous to G2140 1887 DNA Glycine max Predicted polypeptide sequence is orthologous to G2140 1888 DNA Glycine max Predicted polypeptide sequence is orthologous to G2140 1889 DNA Glycine max Predicted polypeptide sequence is orthologous to G2140 1890 DNA Glycine max Predicted polypeptide sequence is orthologous to G2140 1891 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G2140 1892 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G2140 1893 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G2140 1894 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G2140 1895 DNA Zea mays Predicted polypeptide sequence is orthologous to G2140 1896 DNA Lycopersicon Predicted polypeptide esculentum sequence is orthologous to G2140 1897 PRT Oryza sativa Orthologous to G2140 1898 PRT Oryza sativa Orthologous to G2140 1899 PRT Oryza sativa Orthologous to G2140 1900 PRT Oryza sativa Orthologous to G2140 1901 PRT Oryza sativa Orthologous to G2140 1902 DNA Glycine max Predicted polypeptide sequence is orthologous to G2143 1903 DNA Glycine max Predicted polypeptide sequence is orthologous to G2143 1904 DNA Glycine max Predicted polypeptide sequence is orthologous to G2144 1905 DNA Glycine max Predicted polypeptide sequence is orthologous to G2144 1906 DNA Zea mays Predicted polypeptide sequence is orthologous to G2144 1907 DNA Zea mays Predicted polypeptide sequence is orthologous to G2144 1908 DNA Medicago truncatula Predicted polypeptide sequence is orthologous to G2155 1909 DNA Medicago truncatula Predicted polypeptide sequence is orthologous to G2155 1910 DNA Glycine max Predicted polypeptide sequence is orthologous to G2155 1911 PRT Oryza sativa Orthologous to G2192 1912 PRT Oryza sativa Orthologous to G2295 1913 DNA Glycine max Predicted polypeptide sequence is orthologous to G2340 1914 DNA Glycine max Predicted polypeptide sequence is orthologous to G2343 1915 DNA Glycine max Predicted polypeptide sequence is orthologous to G2343 1916 DNA Glycine max Predicted polypeptide sequence is orthologous to G2343 1917 PRT Lycopersicon Orthologous to G2343 esculentum 1918 PRT Oryza sativa Orthologous to G2379 1919 PRT Oryza sativa Orthologous to G2379 1920 PRT Oryza sativa Orthologous to G2379 1921 DNA Glycine max Predicted polypeptide sequence is orthologous to G2505 1922 DNA Zea mays Predicted polypeptide sequence is orthologous to G2505 1923 DNA Glycine max Predicted polypeptide sequence is orthologous to G2520 1924 DNA Glycine max Predicted polypeptide sequence is orthologous to G2520 1925 DNA Oryza sativa Predicted polypeptide sequence is orthologous to G2520 1926 DNA Zea mays Predicted polypeptide sequence is orthologous to G2520 1927 DNA Zea mays Predicted polypeptide sequence is orthologous to G2520 1928 DNA Zea mays Predicted polypeptide sequence is orthologous to G2520 1929 PRT Oryza sativa Orthologous to G2520 1930 PRT Oryza sativa Orthologous to G2520 1931 DNA Glycine max Predicted polypeptide sequence is orthologous to G2557 1932 DNA Glycine max Predicted polypeptide sequence is orthologous to G2557 1933 DNA Glycine max Predicted polypeptide sequence is orthologous to G2557 1934 DNA Zea mays Predicted polypeptide sequence is orthologous to G2557 1935 DNA Zea mays Predicted polypeptide sequence is orthologous to G2557 1936 DNA Glycine max Orthologous to G2557 1937 PRT Oryza sativa Orthologous to G2557 1938 PRT Oryza sativa Orthologous to G2557 1939 PRT Oryza sativa Orthologous to G2557 1940 DNA Glycine max Predicted polypeptide sequence is orthologous to G2719 1941 DNA Zea mays Predicted polypeptide sequence is orthologous to G2719 1942 PRT Oryza sativa Orthologous to G2719 1943 PRT Oryza sativa Orthologous to G2719 1944 DNA Glycine max Predicted polypeptide sequence is orthologous to G2789 1945 DNA Medicago truncatula Predicted polypeptide sequence is orthologous to G2789 1946 DNA Glycine max Predicted polypeptide sequence is orthologous to G2830 1947 G5 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G974 1948 G5 PRT Arabidopsis thaliana Paralogous to G974 1949 G9 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G867, G1930 1950 G9 PRT Arabidopsis thaliana Paralogous to G867, G1930 1951 G12 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G24 1952 G12 PRT Arabidopsis thaliana Paralogous to G24 1953 G30 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1792 1954 G30 PRT Arabidopsis thaliana Paralogous to G1792 1955 G40 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G912, G913 1956 G40 PRT Arabidopsis thaliana Paralogous to G912, G913 1957 G41 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G912, G913 1958 G41 PRT Arabidopsis thaliana Paralogous to G912, G913 1959 G42 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G912, G913 1960 G42 PRT Arabidopsis thaliana Paralogous to G912, G913 1961 G182 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G196 1962 G182 PRT Arabidopsis thaliana Paralogous to G196 1963 G197 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G664 1964 G197 PRT Arabidopsis thaliana Paralogous to G664 1965 G212 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G676 1966 G212 PRT Arabidopsis thaliana Paralogous to G676 1967 G216 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G2719 1968 G216 PRT Arabidopsis thaliana Paralogous to G2719 1969 G221 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1322 1970 G221 PRT Arabidopsis thaliana Paralogous to G1322 1971 G225 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G226, G682 1972 G225 PRT Arabidopsis thaliana Paralogous to G226, G682 1973 G228 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G254 1974 G228 PRT Arabidopsis thaliana Paralogous to G254 1975 G231 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G2007 1976 G231 PRT Arabidopsis thaliana Paralogous to G2007 1977 G233 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G241 1978 G233 PRT Arabidopsis thaliana Paralogous to G241 1979 G247 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G676 1980 G247 PRT Arabidopsis thaliana Paralogous to G676 1981 G249 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1322 1982 G249 PRT Arabidopsis thaliana Paralogous to G1322 1983 G255 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G664 1984 G255 PRT Arabidopsis thaliana Paralogous to G664 1985 G342 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G343 1986 G342 PRT Arabidopsis thaliana Paralogous to G343 1987 G350 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G545 1988 G350 PRT Arabidopsis thaliana Paralogous to G545 1989 G351 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G545 1990 G351 PRT Arabidopsis thaliana Paralogous to G545 1991 G370 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G361, G362 1992 G370 PRT Arabidopsis thaliana Paralogous to G361, G362 1993 G392 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G390, G391, G438 1994 G392 PRT Arabidopsis thaliana Paralogous to G390, G391, G438 1995 G425 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G427 1996 G425 PRT Arabidopsis thaliana Paralogous to G427 1997 G426 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G427 1998 G426 PRT Arabidopsis thaliana Paralogous to G427 1999 G440 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1750 2000 G440 PRT Arabidopsis thaliana Paralogous to G1750 2001 G448 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G450 2002 G448 PRT Arabidopsis thaliana Paralogous to G450 2003 G455 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G450 2004 G455 PRT Arabidopsis thaliana Paralogous to G450 2005 G456 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G450 2006 G456 PRT Arabidopsis thaliana Paralogous to G450 2007 G463 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G464 2008 G463 PRT Arabidopsis thaliana Paralogous to G464 2009 G485 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G481, G482 2010 G485 PRT Arabidopsis thaliana Paralogous to G481, G482 2011 G501 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G519 2012 G501 PRT Arabidopsis thaliana Paralogous to G519 2013 G502 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G519 2014 G502 PRT Arabidopsis thaliana Paralogous to G519 2015 G512 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1452 2016 G512 PRT Arabidopsis thaliana Paralogous to G1452 2017 G515 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G2053 2018 G515 PRT Arabidopsis thaliana Paralogous to G2053 2019 G516 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G2053 2020 G516 PRT Arabidopsis thaliana Paralogous to G2053 2021 G517 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G2053 2022 G517 PRT Arabidopsis thaliana Paralogous to G2053 2023 G554 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1198 2024 G554 PRT Arabidopsis thaliana Paralogous to G1198 2025 G555 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1198 2026 G555 PRT Arabidopsis thaliana Paralogous to G1198 2027 G556 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1198 2028 G556 PRT Arabidopsis thaliana Paralogous to G1198 2029 G558 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1198 2030 G558 PRT Arabidopsis thaliana Paralogous to G1198 2031 G578 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1198 2032 G578 PRT Arabidopsis thaliana Paralogous to G1198 2033 G580 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G568 2034 G580 PRT Arabidopsis thaliana Paralogous to G568 2035 G586 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G585 2036 G586 PRT Arabidopsis thaliana Paralogous to G585 2037 G596 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G2789 2038 G596 PRT Arabidopsis thaliana Paralogous to G2789 2039 G605 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1944 2040 G605 PRT Arabidopsis thaliana Paralogous to G1944 2041 G610 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G849 2042 G610 PRT Arabidopsis thaliana Paralogous to G849 2043 G629 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1198 2044 G629 PRT Arabidopsis thaliana Paralogous to G1198 2045 G659 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1323 2046 G659 PRT Arabidopsis thaliana Paralogous to G1323 2047 G666 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G256 2048 G666 PRT Arabidopsis thaliana Paralogous to G256 2049 G668 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G256 2050 G668 PRT Arabidopsis thaliana Paralogous to G256 2051 G671 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G2340 2052 G671 PRT Arabidopsis thaliana Paralogous to G2340 2053 G714 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G489 2054 G714 PRT Arabidopsis thaliana Paralogous to G489 2055 G729 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1040 2056 G729 PRT Arabidopsis thaliana Paralogous to G1040 2057 G730 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1040 2058 G730 PRT Arabidopsis thaliana Paralogous to G1040 2059 G767 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G519 2060 G767 PRT Arabidopsis thaliana Paralogous to G519 2061 G839 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1196 2062 G839 PRT Arabidopsis thaliana Paralogous to G1196 2063 G861 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1140 2064 G861 PRT Arabidopsis thaliana Paralogous to G1140 2065 G932 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G256 2066 G932 PRT Arabidopsis thaliana Paralogous to G256 2067 G986 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G881 2068 G986 PRT Arabidopsis thaliana Paralogous to G881 2069 G990 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1451 2070 G990 PRT Arabidopsis thaliana Paralogous to G1451 2071 G993 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G867, G1930 2072 G993 PRT Arabidopsis thaliana Paralogous to G867, G1930 2073 G1006 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G28 2074 G1006 PRT Arabidopsis thaliana Paralogous to G28 2075 G1008 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G2130 2076 G1008 PRT Arabidopsis thaliana Paralogous to G2130 2077 G1067 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1073 2078 G1067 PRT Arabidopsis thaliana Paralogous to G1073 2079 G1076 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1075 2080 G1076 PRT Arabidopsis thaliana Paralogous to G1075 2081 G1136 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G584 2082 G1136 PRT Arabidopsis thaliana Paralogous to G584 2083 G1149 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1146 2084 G1149 PRT Arabidopsis thaliana Paralogous to G1146 2085 G1152 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1146 2086 G1152 PRT Arabidopsis thaliana Paralogous to G1146 2087 G1211 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G291 2088 G1211 PRT Arabidopsis thaliana Paralogous to G291 2089 G1277 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G24 2090 G1277 PRT Arabidopsis thaliana Paralogous to G24 2091 G1290 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G278 2092 G1290 PRT Arabidopsis thaliana Paralogous to G278 2093 G1329 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G663 2094 G1329 PRT Arabidopsis thaliana Paralogous to G663 2095 G1335 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G652 2096 G1335 PRT Arabidopsis thaliana Paralogous to G652 2097 G1349 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G896 2098 G1349 PRT Arabidopsis thaliana Paralogous to G896 2099 G1357 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1452 2100 G1357 PRT Arabidopsis thaliana Paralogous to G1452 2101 G1364 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G481, G482 2102 G1364 PRT Arabidopsis thaliana Paralogous to G481, G482 2103 G1379 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G24 2104 G1379 PRT Arabidopsis thaliana Paralogous to G24 2105 G1387 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G975, G2583 2106 G1387 PRT Arabidopsis thaliana Paralogous to G975, G2583 2107 G1425 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G504 2108 G1425 PRT Arabidopsis thaliana Paralogous to G504 2109 G1454 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G504 2110 G1454 PRT Arabidopsis thaliana Paralogous to G504 2111 G1456 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1927 2112 G1456 PRT Arabidopsis thaliana Paralogous to G1927 2113 G1461 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1463 2114 G1461 PRT Arabidopsis thaliana Paralogous to G1463 2115 G1462 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1463 2116 G1462 PRT Arabidopsis thaliana Paralogous to G1463 2117 G1464 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1463 2118 G1464 PRT Arabidopsis thaliana Paralogous to G1463 2119 G1465 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1463 2120 G1465 PRT Arabidopsis thaliana Paralogous to G1463 2121 G1484 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1255 2122 G1484 PRT Arabidopsis thaliana Paralogous to G1255 2123 G1548 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G390, G391, G438 2124 G1548 PRT Arabidopsis thaliana Paralogous to G390, G391, G438 2125 G1646 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G715 2126 G1646 PRT Arabidopsis thaliana Paralogous to G715 2127 G1664 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1062 2128 G1664 PRT Arabidopsis thaliana Paralogous to G1062 2129 G1759 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G157, G859, G1842, G1843 2130 G1759 PRT Arabidopsis thaliana Paralogous to G157, G859, G1842, G1843 2131 G1782 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1363 2132 G1782 PRT Arabidopsis thaliana Paralogous to G1363 2133 G1791 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1792 2134 G1791 PRT Arabidopsis thaliana Paralogous to G1792 2135 G1795 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1792 2136 G1795 PRT Arabidopsis thaliana Paralogous to G1792 2137 G1806 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1198 2138 G1806 PRT Arabidopsis thaliana Paralogous to G1198 2139 G1808 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1047 2140 G1808 PRT Arabidopsis thaliana Paralogous to G1047 2141 G1816 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G226, G682 2142 G1816 PRT Arabidopsis thaliana Paralogous to G226, G682 2143 G1839 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1749 2144 G1839 PRT Arabidopsis thaliana Paralogous to G1749 2145 G1844 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G157, G859, G1842, G1843 2146 G1844 PRT Arabidopsis thaliana Paralogous to G157, G859, G1842, G1843 2147 G1888 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1482 2148 G1888 PRT Arabidopsis thaliana Paralogous to G1482 2149 G1889 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G353, G354 2150 G1889 PRT Arabidopsis thaliana Paralogous to G353, G354 2151 G1929 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1478 2152 G1929 PRT Arabidopsis thaliana Paralogous to G1478 2153 G1945 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G2155 2154 G1945 PRT Arabidopsis thaliana Paralogous to G2155 2155 G1974 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G353, G354 2156 G1974 PRT Arabidopsis thaliana Paralogous to G353, G354 2157 G1995 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G361, G362 2158 G1995 PRT Arabidopsis thaliana Paralogous to G361, G362 2159 G1998 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G325 2160 G1998 PRT Arabidopsis thaliana Paralogous to G325 2161 G2107 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G912, G913 2162 G2107 PRT Arabidopsis thaliana Paralogous to G912, G913 2163 G2131 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G979 2164 G2131 PRT Arabidopsis thaliana Paralogous to G979 2165 G2156 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1073 2166 G2156 PRT Arabidopsis thaliana Paralogous to G1073 2167 G2184 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1927 2168 G2184 PRT Arabidopsis thaliana Paralogous to G1927 2169 G2334 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1863 2170 G2334 PRT Arabidopsis thaliana Paralogous to G1863 2171 G2345 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G481, G482 2172 G2345 PRT Arabidopsis thaliana Paralogous to G481, G482 2173 G2421 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G663 2174 G2421 PRT Arabidopsis thaliana Paralogous to G663 2175 G2422 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G663 2176 G2422 PRT Arabidopsis thaliana Paralogous to G663 2177 G2423 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1330 2178 G2423 PRT Arabidopsis thaliana Paralogous to G1330 2179 G2424 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1645 2180 G2424 PRT Arabidopsis thaliana Paralogous to G1645 2181 G2432 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G736 2182 G2432 PRT Arabidopsis thaliana Paralogous to G736 2183 G2513 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G912, G913 2184 G2513 PRT Arabidopsis thaliana Paralogous to G912, G913 2185 G2535 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G961 2186 G2535 PRT Arabidopsis thaliana Paralogous to G961 2187 G2545 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G427 2188 G2545 PRT Arabidopsis thaliana Paralogous to G427 2189 G2631 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G484 2190 G2631 PRT Arabidopsis thaliana Paralogous to G484 2191 G2718 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G226, G682 2192 G2718 PRT Arabidopsis thaliana Paralogous to G226, G682 2193 G2776 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G1652 2194 G2776 PRT Arabidopsis thaliana Paralogous to G1652 2195 G2826 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G361, G362 2196 G2826 PRT Arabidopsis thaliana Paralogous to G361, G362 2197 G2838 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G361, G362 2198 G2838 PRT Arabidopsis thaliana Paralogous to G361, G362 2199 G2839 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G353, G354 2200 G2839 PRT Arabidopsis thaliana Paralogous to G353, G354 2201 G3010 DNA Arabidopsis thaliana Predicted polypeptide sequence is paralogous to G987 2202 G3010 PRT Arabidopsis thaliana Paralogous to G987 Molecular Modeling

Another means that may be used to confirm the utility and function of transcription factor sequences that are orthologous or paralogous to presently disclosed transcription factors is through the use of molecular modeling software. Molecular modeling is routinely used to predict polypeptide structure, and a variety of protein structure modeling programs, such as “Insight II” (Accelrys, Inc.) are commercially available for this purpose. Modeling can thus be used to predict which residues of a polypeptide can be changed without altering function (Crameri et al. (2003) U.S. Pat. No. 6,521,453). Thus, polypeptides that are sequentially similar can be shown to have a high likelihood of similar function by their structural similarity, which may, for example, be established by comparison of regions of superstructure. The relative tendencies of amino acids to form regions of superstructure (for example, helixes and β-sheets) are well established. For example, O'Neil et al. (1990) Science 250: 646-651) have discussed in detail the helix forming tendencies of amino acids. Tables of relative structure forming activity for amino acids can be used as substitution tables to predict which residues can be functionally substituted in a given region, for example, in DNA-binding domains of known transcription factors and equivalogs. Homologs that are likely to be functionally similar can then be identified.

Of particular interest is the structure of a transcription factor in the region of its conserved domain, such as those identified in Table 5. Structural analyses may be performed by comparing the structure of the known transcription factor around its conserved domain with those of orthologs and paralogs. Analysis of a number of polypeptides within a transcription factor group or clade, including the functionally or sequentially similar polypeptides provided in the Sequence Listing, may also provide an understanding of structural elements required to regulate transcription within a given family.

EXAMPLES

The invention, now being generally described, will be more readily understood by reference to the following examples, which are included merely for purposes of illustration of certain aspects and embodiments of the present invention and are not intended to limit the invention. It will be recognized by one of skill in the art that a transcription factor that is associated with a particular first trait may also be associated with at least one other, unrelated and inherent second trait which was not predicted by the first trait.

The complete descriptions of the traits associated with each polynucleotide of the invention are fully disclosed in Table 4 and Table 6. The complete description of the transcription factor gene family and identified conserved domains of the polypeptide encoded by the polynucleotide is fully disclosed in Table 5.

Example I Full Length Gene Identification and Cloning

Putative transcription factor sequences (genomic or ESTs) related to known transcription factors were identified in the Arabidopsis thaliana GenBank database using the tblastn sequence analysis program using default parameters and a P-value cutoff threshold of −4 or −5 or lower, depending on the length of the query sequence. Putative transcription factor sequence hits were then screened to identify those containing particular sequence strings. If the sequence hits contained such sequence strings, the sequences were confirmed as transcription factors.

Alternatively, Arabidopsis thaliana cDNA libraries derived from different tissues or treatments, or genomic libraries were screened to identify novel members of a transcription family using a low stringency hybridization approach. Probes were synthesized using gene specific primers in a standard PCR reaction (annealing temperature 60° C.) and labeled with ³²P dCTP using the High Prime DNA Labeling Kit (Boehringer Mannheim Corp. (now Roche Diagnostics Corp., Indianapolis, Ind.). Purified radiolabelled probes were added to filters immersed in Church hybridization medium (0.5 M NaPO₄ pH 7.0, 7% SDS, 1% w/v bovine serum albumin) and hybridized overnight at 60° C. with shaking. Filters were washed two times for 45 to 60 minutes with 1×SCC, 1% SDS at 60° C.

To identify additional sequence 5′ or 3′ of a partial cDNA sequence in a cDNA library, 5′ and 3′ rapid amplification of cDNA ends (RACE) was performed using the MARATHON cDNA amplification kit (Clontech, Palo Alto, Calif.). Generally, the method entailed first isolating poly(A) mRNA, performing first and second strand cDNA synthesis to generate double stranded cDNA, blunting cDNA ends, followed by ligation of the MARATHON Adaptor to the cDNA to form a library of adaptor-ligated ds cDNA.

Gene-specific primers were designed to be used along with adaptor specific primers for both 5′ and 3′ RACE reactions. Nested primers, rather than single primers, were used to increase PCR specificity. Using 5′ and 3′ RACE reactions, 5′ and 3′ RACE fragments were obtained, sequenced and cloned. The process can be repeated until 5′ and 3′ ends of the full-length gene were identified. Then the full-length cDNA was generated by PCR using primers specific to 5′ and 3′ ends of the gene by end-to-end PCR.

Example II Construction of Expression Vectors

The sequence was amplified from a genomic or cDNA library using primers specific to sequences upstream and downstream of the coding region. The expression vector was pMEN20 or pMEN65, which are both derived from pMON316 (Sanders et al. (1987) Nucleic Acids Res. 15:1543-1558) and contain the CaMV 35S promoter to express transgenes. To clone the sequence into the vector, both pMEN20 and the amplified DNA fragment were digested separately with SalI and NotI restriction enzymes at 37° C. for 2 hours. The digestion products were subject to electrophoresis in a 0.8% agarose gel and visualized by ethidium bromide staining. The DNA fragments containing the sequence and the linearized plasmid were excised and purified by using a QIAQUICK gel extraction kit (Qiagen, Valencia Calif.). The fragments of interest were ligated at a ratio of 3:1 (vector to insert). Ligation reactions using T4 DNA ligase (New England Biolabs, Beverly Mass.) were carried out at 16° C. for 16 hours. The ligated DNAs were transformed into competent cells of the E. coli strain DH5alpha by using the heat shock method. The transformations were plated on LB plates containing 50 mg/l kanamycin (Sigma Chemical Co. St. Louis Mo.). Individual colonies were grown overnight in five milliliters of LB broth containing 50 mg/l kanamycin at 37° C. Plasmid DNA was purified by using Qiaquick Mini Prep kits (Qiagen).

Example III Transformation of Agrobacterium with the Expression Vector

After the plasmid vector containing the gene was constructed, the vector was used to transform Agrobacterium tumefaciens cells expressing the gene products. The stock of Agrobacterium tumefaciens cells for transformation were made as described by Nagel et al. (1990) FEMS Microbiol Letts. 67: 325-328. Agrobacterium strain ABI was grown in 250 ml LB medium (Sigma) overnight at 28° C. with shaking until an absorbance over 1 cm at 600 nm (A₆₀₀) of 0.5-1.0 was reached. Cells were harvested by centrifugation at 4,000×g for 15 min at 4° C. Cells were then resuspended in 250 μl chilled buffer (1 mM HEPES, pH adjusted to 7.0 with KOH). Cells were centrifuged again as described above and resuspended in 125 μl chilled buffer. Cells were then centrifuged and resuspended two more times in the same HEPES buffer as described above at a volume of 100 μl and 750 μl, respectively. Resuspended cells were then distributed into 40 μl aliquots, quickly frozen in liquid nitrogen, and stored at −80° C.

Agrobacterium cells were transformed with plasmids prepared as described above following the protocol described by Nagel et al. (supra). For each DNA construct to be transformed, 50-100 ng DNA (generally resuspended in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0) was mixed with 40 μl of Agrobacterium cells. The DNA/cell mixture was then transferred to a chilled cuvette with a 2 mm electrode gap and subject to a 2.5 kV charge dissipated at 25 μF and 200 μF using a Gene Pulser II apparatus (Bio-Rad, Hercules, Calif.). After electroporation, cells were immediately resuspended in 1.0 ml LB and allowed to recover without antibiotic selection for 2-4 hours at 28° C. in a shaking incubator. After recovery, cells were plated onto selective medium of LB broth containing 100 μg/ml spectinomycin (Sigma) and incubated for 24-48 hours at 28° C. Single colonies were then picked and inoculated in fresh medium. The presence of the plasmid construct was verified by PCR amplification and sequence analysis.

Example IV Transformation of Arabidopsis Plants with Agrobacterium tumefaciens with Expression Vector

After transformation of Agrobacterium tumefaciens with plasmid vectors containing the gene, single Agrobacterium colonies were identified, propagated, and used to transform Arabidopsis plants. Briefly, 500 ml cultures of LB medium containing 50 mg/l kanamycin were inoculated with the colonies and grown at 28° C. with shaking for 2 days until an optical absorbance at 600 nm wavelength over 1 cm (A₆₀₀) of >2.0 is reached. Cells were then harvested by centrifugation at 4,000×g for 10 min, and resuspended in infiltration medium (½× Murashige and Skoog salts (Sigma), 1× Gamborg's B-5 vitamins (Sigma), 5.0% (w/v) sucrose (Sigma), 0.044 μM benzylamino purine (Sigma), 200 μl/l Silwet L-77 (Lehle Seeds) until an A₆₀₀ of 0.8 was reached.

Prior to transformation, Arabidopsis thaliana seeds (ecotype Columbia) were sown at a density of ˜10 plants per 4″ pot onto Pro-Mix BX potting medium (Hummert International) covered with fiberglass mesh (18 mm×16 mm). Plants were grown under continuous illumination (50-75 μE/m²/sec) at 22-23° C. with 65-70% relative humidity. After about 4 weeks, primary inflorescence stems (bolts) are cut off to encourage growth of multiple secondary bolts. After flowering of the mature secondary bolts, plants were prepared for transformation by removal of all siliques and opened flowers.

The pots were then immersed upside down in the mixture of Agrobacterium infiltration medium as described above for 30 sec, and placed on their sides to allow draining into a 1′×2′ flat surface covered with plastic wrap. After 24 h, the plastic wrap was removed and pots are turned upright. The immersion procedure was repeated one week later, for a total of two immersions per pot. Seeds were then collected from each transformation pot and analyzed following the protocol described below.

Example V Identification of Arabidopsis Primary Transformants

Seeds collected from the transformation pots were sterilized essentially as follows. Seeds were dispersed into in a solution containing 0.1% (v/v) Triton X-100 (Sigma) and sterile water and washed by shaking the suspension for 20 min. The wash solution was then drained and replaced with fresh wash solution to wash the seeds for 20 min with shaking. After removal of the ethanol/detergent solution, a solution containing 0.1% (v/v) Triton X-100 and 30% (v/v) bleach (CLOROX; Clorox Corp. Oakland Calif.) was added to the seeds, and the suspension was shaken for 10 min. After removal of the bleach/detergent solution, seeds were then washed five times in sterile distilled water. The seeds were stored in the last wash water at 4° C. for 2 days in the dark before being plated onto antibiotic selection medium (1× Murashige and Skoog salts (pH adjusted to 5.7 with 1M KOH), 1× Gamborg's B-5 vitamins, 0.9% phytagar (Life Technologies), and 50 mg/l kanamycin). Seeds were germinated under continuous illumination (50-75 μE/m²/sec) at 22-23° C. After 7-10 days of growth under these conditions, kanamycin resistant primary transformants (T₁ generation) were visible and obtained. These seedlings were transferred first to fresh selection plates where the seedlings continued to grow for 3-5 more days, and then to soil (Pro-Mix BX potting medium).

Primary transformants were crossed and progeny seeds (T₂) collected; kanamycin resistant seedlings were selected and analyzed. The expression levels of the recombinant polynucleotides in the transformants varies from about a 5% expression level increase to a least a 100% expression level increase. Similar observations are made with respect to polypeptide level expression.

Example VI Identification of Arabidopsis Plants with Transcription Factor Gene Knockouts

The screening of insertion mutagenized Arabidopsis collections for null mutants in a known target gene was essentially as described in Krysan et al. (1999) Plant Cell 11: 2283-2290. Briefly, gene-specific primers, nested by 5-250 base pairs to each other, were designed from the 5′ and 3′ regions of a known target gene. Similarly, nested sets of primers were also created specific to each of the T-DNA or transposon ends (the “right” and “left” borders). All possible combinations of gene specific and T-DNA/transposon primers were used to detect by PCR an insertion event within or close to the target gene. The amplified DNA fragments were then sequenced which allows the precise determination of the T-DNA/transposon insertion point relative to the target gene. Insertion events within the coding or intervening sequence of the genes were deconvoluted from a pool comprising a plurality of insertion events to a single unique mutant plant for functional characterization. The method is described in more detail in Yu and Adam, U.S. application Ser. No. 09/177,733 filed Oct. 23, 1998.

Example VII Identification of Modified Phenotypes in Overexpression or Gene Knockout Plants

Experiments were performed to identify those transformants or knockouts that exhibited modified biochemical characteristics. Among the biochemicals that were assayed were insoluble sugars, such as arabinose, fucose, galactose, mannose, rhamnose or xylose or the like; prenyl lipids, such as lutein, beta-carotene, xanthophyll-1, xanthophyll-2, chlorophylls A or B, or alpha-, delta- or gamma-tocopherol or the like; fatty acids, such as 16:0 (palmitic acid), 16:1 (palmitoleic acid), 18:0 (stearic acid), 18:1 (oleic acid), 18:2 (linoleic acid), 20:0, 18:3 (linolenic acid), 20:1 (eicosenoic acid), 20:2, 22:1 (erucic acid) or the like; waxes, such as by altering the levels of C29, C31, or C33 alkanes; sterols, such as brassicasterol, campesterol, stigmasterol, sitosterol or stigmastanol or the like, glucosinolates, protein or oil levels.

Fatty acids were measured using two methods depending on whether the tissue was from leaves or seeds. For leaves, lipids were extracted and esterified with hot methanolic H₂SO₄ and partitioned into hexane from methanolic brine. For seed fatty acids, seeds were pulverized and extracted in methanol:heptane:toluene:2,2-dimethoxypropane:H₂SO₄ (39:34:20:5:2) for 90 minutes at 80° C. After cooling to room temperature the upper phase, containing the seed fatty acid esters, was subjected to GC analysis. Fatty acid esters from both seed and leaf tissues were analyzed with a SUPELCO SP-2330 column (Supelco, Bellefonte, Pa.).

Glucosinolates were purified from seeds or leaves by first heating the tissue at 95° C. for 10 minutes. Preheated ethanol:water (50:50) is added and after heating at 95° C. for a further 10 minutes, the extraction solvent is applied to a DEAE Sephadex column (Pharmacia) which had been previously equilibrated with 0.5 M pyridine acetate. Desulfoglucosinolates were eluted with 300 ul water and analyzed by reverse phase HPLC monitoring at 226 nm.

For wax alkanes, samples were extracted using an identical method as fatty acids and extracts were analyzed on a HP 5890 GC coupled with a 5973 MSD. Samples were chromatographically isolated on a J&W DB35 mass spectrometer (J&W Scientific Agilent Technologies, Folsom, Calif.).

To measure prenyl lipid levels, seeds or leaves were pulverized with 1 to 2% pyrogallol as an antioxidant. For seeds, extracted samples were filtered and a portion removed for tocopherol and carotenoid/chlorophyll analysis by HPLC. The remaining material was saponified for sterol determination. For leaves, an aliquot was removed and diluted with methanol and chlorophyll A, chlorophyll B, and total carotenoids measured by spectrophotometry by determining optical absorbance at 665.2 nm, 652.5 nm, and 470 nm. An aliquot was removed for tocopherol and carotenoid/chlorophyll composition by HPLC using a Waters μBondapak C18 column (4.6 mm×150 mm). The remaining methanolic solution was saponified with 10% KOH at 80° C. for one hour. The samples were cooled and diluted with a mixture of methanol and water. A solution of 2% methylene chloride in hexane was mixed in and the samples were centrifuged. The aqueous methanol phase was again re-extracted 2% methylene chloride in hexane and, after centrifugation, the two upper phases were combined and evaporated. 2% methylene chloride in hexane was added to the tubes and the samples were then extracted with one ml of water. The upper phase was removed, dried, and resuspended in 400 ul of 2% methylene chloride in hexane and analyzed by gas chromatography using a 50 m DB-5 ms (0.25 mm ID, 0.25 um phase, J&W Scientific).

Insoluble sugar levels were measured by the method essentially described by Reiter et al. (1999), Plant J. 12: 335-345. This method analyzes the neutral sugar composition of cell wall polymers found in Arabidopsis leaves. Soluble sugars were separated from sugar polymers by extracting leaves with hot 70% ethanol. The remaining residue containing the insoluble polysaccharides was then acid hydrolyzed with allose added as an internal standard. Sugar monomers generated by the hydrolysis were then reduced to the corresponding alditols by treatment with NaBH4, then were acetylated to generate the volatile alditol acetates which were then analyzed by GC-FID. Identity of the peaks was determined by comparing the retention times of known sugars converted to the corresponding alditol acetates with the retention times of peaks from wild-type plant extracts. Alditol acetates were analyzed on a Supelco SP-2330 capillary column (30 m×250 μm×0.2 μm) using a temperature program beginning at 180° C. for 2 minutes followed by an increase to 220° C. in 4 minutes. After holding at 220° C. for 10 minutes, the oven temperature is increased to 240° C. in 2 minutes and held at this temperature for 10 minutes and brought back to room temperature.

To identify plants with alterations in total seed oil or protein content, 150 mg of seeds from T2 progeny plants were subjected to analysis by Near Infrared Reflectance Spectroscopy SIRS) using a Foss NirSystems Model 6500 with a spinning cup transport system. NIRS is a non-destructive analytical method used to determine seed oil and protein composition. Infrared is the region of the electromagnetic spectrum located after the visible region in the direction of longer wavelengths. ‘Near infrared’ owns its name for being the infrared region near to the visible region of the electromagnetic spectrum. For practical purposes, near infrared comprises wavelengths between 800 and 2500 nm. NIRS is applied to organic compounds rich in O—H bonds (such as moisture, carbohydrates, and fats), C—H bonds (such as organic compounds and petroleum derivatives), and N—H bonds (such as proteins and amino acids). The NIRS analytical instruments operate by statistically correlating NIRS signals at several wavelengths with the characteristic or property intended to be measured. All biological substances contain thousands of C—H, O—H, and N—H bonds. Therefore, the exposure to near infrared radiation of a biological sample, such as a seed, results in a complex spectrum which contains qualitative and quantitative information about the physical and chemical composition of that sample.

The numerical value of a specific analyte in the sample, such as protein content or oil content, is mediated by a calibration approach known as chemometrics. Chemometrics applies statistical methods such as multiple linear regression (MLR), partial least squares (PLS), and principle component analysis (PCA) to the spectral data and correlates them with a physical property or other factor, that property or factor is directly determined rather than the analyte concentration itself. The method first provides “wet chemistry” data of the samples required to develop the calibration.

Calibration of NIRS response was performed using data obtained by wet chemical analysis of a population of Arabidopsis ecotypes that were expected to represent diversity of oil and protein levels.

The exact oil composition of each ecotype used in the calibration experiment was performed using gravimetric analysis of oils extracted from seed samples (0.5 g or 1.0 g) by the accelerated solvent extraction method (ASE; Dionex Corp, Sunnyvale, Calif.). The extraction method was validated against certified canola samples (Community Bureau of Reference, Belgium). Seed samples from each ecotype (0.5 g or 1 g) were subjected to accelerated solvent extraction and the resulting extracted oil weights compared to the weight of oil recovered from canola seed that has been certified for oil content (Community Bureau of Reference). The oil calibration equation was based on 57 samples with a range of oil contents from 27.0% to 50.8%. To check the validity of the calibration curve, an additional set of samples was extracted by ASE and predicted using the oil calibration equation. This validation set counted 46 samples, ranging from 27.9% to 47.5% oil, and had a predicted standard error of performance of 0.63%. The wet chemical method for protein was elemental analysis (% N×6.0) using the average of 3 representative samples of 5 mg each validated against certified ground corn (NIST). The instrumentation was an Elementar Vario-EL III elemental analyzer operated in CNS operating mode (Elementar Analysensysteme GmbH, Hanau, Germany).

The protein calibration equation was based on a library of 63 samples with a range of protein contents from 17.4% to 31.2%. An additional set of samples was analyzed for protein by elemental analysis (n=57) and scanned by NIRS in order to validate the protein prediction equation. The protein range of the validation set was from 16.8% to 31.2% and the standard error of prediction was 0.468%.

NIRS analysis of Arabidopsis seed was carried out on between 40-300 mg experimental sample. The oil and protein contents were predicted using the respective calibration equations.

Data obtained from NIRS analysis was analyzed statistically using a nearest-neighbor (N—N) analysis. The N—N analysis allows removal of within-block spatial variability in a fairly flexible fashion, which does not require prior knowledge of the pattern of variability in the chamber. Ideally, all hybrids are grown under identical experimental conditions within a block (rep). In reality, even in many block designs, significant within-block variability exists. Nearest-neighbor procedures are based on assumption that environmental effect of a plot is closely related to that of its neighbors. Nearest-neighbor methods use information from adjacent plots to adjust for within-block heterogeneity and so provide more precise estimates of treatment means and differences. If there is within-plot heterogeneity on a spatial scale that is larger than a single plot and smaller than the entire block, then yields from adjacent plots will be positively correlated. Information from neighboring plots can be used to reduce or remove the unwanted effect of the spatial heterogeneity, and hence improve the estimate of the treatment effect. Data from neighboring plots can also be used to reduce the influence of competition between adjacent plots. The Papadakis N—N analysis can be used with designs to remove within-block variability that would not be removed with the standard split plot analysis (Papadakis (1973) Inst. d'Amelior. Plantes Thessaloniki (Greece) Bull. Scientif. No. 23; Papadakis (1984) Proc. Acad. Athens 59: 326-342.

Experiments were performed to identify those transformants or knockouts that exhibited modified sugar-sensing. For such studies, seeds from transformants were germinated on media containing 5% glucose or 9.4% sucrose which normally partially restrict hypocotyl elongation. Plants with altered sugar sensing may have either longer or shorter hypocotyls than normal plants when grown on this media. Additionally, other plant traits may be varied such as root mass.

Experiments may be performed to identify those transformants or knockouts that exhibited an improved pathogen tolerance. For such studies, the transformants are exposed to biotropic fungal pathogens, such as Erysiphe orontii, and necrotropic fungal pathogens, such as Fusarium oxysporum. Fusarium oxysporum isolates cause vascular wilts and damping off of various annual vegetables, perennials and weeds (Mauch-Mani and Slusarenko (1994) Molec Plant-Microbe Interact. 7: 378-383). For Fusarium oxysporum experiments, plants are grown on Petri dishes and sprayed with a fresh spore suspension of F. oxysporum. The spore suspension is prepared as follows: A plug of fungal hyphae from a plate culture is placed on a fresh potato dextrose agar plate and allowed to spread for one week. Five ml sterile water is then added to the plate, swirled, and pipetted into 50 ml Armstrong Fusarium medium. Spores are grown overnight in Fusarium medium and then sprayed onto plants using a Preval paint sprayer. Plant tissue is harvested and frozen in liquid nitrogen 48 hours post-infection.

Erysiphe orontii is a causal agent of powdery mildew. For Erysiphe orontii experiments, plants are grown approximately 4 weeks in a greenhouse under 12 hour light (20° C., 30% relative humidity (rh)). Individual leaves are infected with E. orontii spores from infected plants using a camel's hair brush, and the plants are transferred to a Percival growth chamber (20° C., 80% rh.). Plant tissue is harvested and frozen in liquid nitrogen 7 days post-infection.

Botrytis cinerea is a necrotrophic pathogen. Botrytis cinerea is grown on potato dextrose agar under 12 hour light (20° C., 30% relative humidity (rh)). A spore culture is made by spreading 10 ml of sterile water on the fungus plate, swirling and transferring spores to 10 ml of sterile water. The spore inoculum (approx. 105 spores/ml) is then used to spray 10 day-old seedlings grown under sterile conditions on MS (minus sucrose) media. Symptoms are evaluated every day up to approximately 1 week.

Sclerotinia sclerotiorum hyphal cultures are grown in potato dextrose broth. One gram of hyphae is ground, filtered, spun down and resuspended in sterile water. A 1:10 dilution is used to spray 10 day-old seedlings grown aseptically under a 12 hour light/dark regime on MS (minus sucrose) media. Symptoms are evaluated every day up to approximately 1 week.

Pseudomonas syringae pv maculicola (Psm) strain 4326 and pv maculicola strain 4326 was inoculated by hand at two doses. Two inoculation doses allows the differentiation between plants with enhanced susceptibility and plants with enhanced resistance to the pathogen. Plants are grown for 3 weeks in the greenhouse, then transferred to the growth chamber for the remainder of their growth. Psm ES4326 may be hand inoculated with 1 ml syringe on 3 fully-expanded leaves per plant (4½ wk old), using at least 9 plants per overexpressing line at two inoculation doses, OD=0.005 and OD=0.0005. Disease scoring is performed at day 3 post-inoculation with pictures of the plants and leaves taken in parallel.

In some instances, expression patterns of the pathogen-induced genes (such as defense genes) may be monitored by microarray experiments. In these experiments, cDNAs are generated by PCR and resuspended at a final concentration of ˜100 ng/μl in 3×SSC or 150 mM Na-phosphate (Eisen and Brown (1999) Methods Enzymol. 303: 179-205). The cDNAs are spotted on microscope glass slides coated with polylysine. The prepared cDNAs are aliquoted into 384 well plates and spotted on the slides using, for example, an x-y-z gantry (OmniGrid) which may be purchased from GeneMachines (Menlo Park, Calif.) outfitted with quill type pins which may be purchased from Telechem International (Sunnyvale, Calif.). After spotting, the arrays are cured for a minimum of one week at room temperature, rehydrated and blocked following the protocol recommended by Eisen and Brown (1999; supra).

Sample total RNA (10 μg) samples are labeled using fluorescent Cy3 and Cy5 dyes. Labeled samples are resuspended in 4×SSC/0.03% SDS/4 μg salmon sperm DNA/2 μg tRNA/50 mM Na-pyrophosphate, heated for 95° C. for 2.5 minutes, spun down and placed on the array. The array is then covered with a glass coverslip and placed in a sealed chamber. The chamber is then kept in a water bath at 62° C. overnight. The arrays are washed as described in Eisen and Brown (1999, supra) and scanned on a General Scanning 3000 laser scanner. The resulting files are subsequently quantified using IMAGENE, software (BioDiscovery, Los Angeles Calif.).

RT-PCR experiments may be performed to identify those genes induced after exposure to biotropic fungal pathogens, such as Erysiphe orontii, necrotropic fungal pathogens, such as Fusarium oxysporum, bacteria, viruses and salicylic acid, the latter being involved in a nonspecific resistance response in Arabidopsis thaliana. Generally, the gene expression patterns from ground plant leaf tissue is examined.

Reverse transcriptase PCR was conducted using gene specific primers within the coding region for each sequence identified. The primers were designed near the 3′ region of each DNA binding sequence initially identified.

Total RNA from these ground leaf tissues was isolated using the CTAB extraction protocol. Once extracted total RNA was normalized in concentration across all the tissue types to ensure that the PCR reaction for each tissue received the same amount of cDNA template using the 28S band as reference. Poly(A+) RNA was purified using a modified protocol from the Qiagen OLIGOTEX purification kit batch protocol. cDNA was synthesized using standard protocols. After the first strand cDNA synthesis, primers for Actin 2 were used to normalize the concentration of cDNA across the tissue types. Actin 2 is found to be constitutively expressed in fairly equal levels across the tissue types being investigated.

For RT PCR, cDNA template was mixed with corresponding primers and Taq DNA polymerase. Each reaction consisted of 0.2 μl cDNA template, 2 μl 10× Tricine buffer, 2 μl 10× Tricine buffer and 16.8 μl water, 0.05 μl Primer 1, 0.05 μl, Primer 2, 0.3 μl Taq DNA polymerase and 8.6 μl water.

The 96 well plate is covered with microfilm and set in the thermocycler to start the reaction cycle. By way of illustration, the reaction cycle may comprise the following steps:

STEP 1: 93° C. FOR 3 MIN;

Step 2: 93° C. for 30 sec;

Step 3: 65° C. for 1 min;

Step 4: 72° C. for 2 min;

Steps 2, 3 and 4 are repeated for 28 cycles;

Step 5: 72° C. for 5 min; and

Step 6 4° C.

To amplify more products, for example, to identify genes that have very low expression, additional steps may be performed: The following method illustrates a method that may be used in this regard. The PCR plate is placed back in the thermocycler for 8 more cycles of steps 2-4.

Step 2 93° C. for 30 sec;

Step 3 65° C. for 1 min;

Step 4 72° C. for 2 min, repeated for 8 cycles; and

Step 5 4° C.

Eight microliters of PCR product and 1.5 μl of loading dye are loaded on a 1.2% agarose gel for analysis after 28 cycles and 36 cycles. Expression levels of specific transcripts are considered low if they were only detectable after 36 cycles of PCR. Expression levels are considered medium or high depending on the levels of transcript compared with observed transcript levels for an internal control such as actin2. Transcript levels are determined in repeat experiments and compared to transcript levels in control (e.g., non-transformed) plants.

Experiments were performed to identify those transformants or knockouts that exhibited an improved environmental stress tolerance. For such studies, the transformants were exposed to a variety of environmental stresses. Plants were exposed to chilling stress (6 hour exposure to 4-8° C.), heat stress (6 hour exposure to 32-37° C.), high salt stress (6 hour exposure to 200 mM NaCl), drought stress (168 hours after removing water from trays), osmotic stress (6 hour exposure to 3 M mannitol), or nutrient limitation (nitrogen: all components of MS medium remained constant except N was reduced to 20 mg/l of NH₄NO₃; phosphate: all components of MS medium except KH2PO₄, which was replaced by K₂SO₄; potassium: all components of MS medium except removal of KNO₃ and KH₂PO₄, which were replaced by NaH₄PO₄).

Experiments were performed to identify those transformants or knockouts that exhibited a modified structure and development characteristics. For such studies, the transformants were observed by eye to identify novel structural or developmental characteristics associated with the ectopic expression of the polynucleotides or polypeptides of the invention.

Flowering time was measured by the number of rosette leaves present when a visible inflorescence of approximately 3 cm is apparent. Rosette and total leaf number on the progeny stem are tightly correlated with the timing of flowering (Koornneef et al. (1991) Mol. Gen. Genet. 229: 57-66). The vernalization response was also measured. For vernalization treatments, seeds were sown to MS agar plates, sealed with micropore tape, and placed in a 4° C. cold room with low light levels for 6-8 weeks. The plates were then transferred to the growth rooms alongside plates containing freshly sown non-vernalized controls. Rosette leaves were counted when a visible inflorescence of approximately 3 cm was apparent.

Modified phenotypes observed for particular overexpressor or knockout plants are provided in Table 4. For a particular overexpressor that shows a less beneficial characteristic, it may be more useful to select a plant with a decreased expression of the particular transcription factor. For a particular knockout that shows a less beneficial characteristic, it may be more useful to select a plant with an increased expression of the particular transcription factor.

The sequences of the Sequence Listing or those in Tables 4-8, or those disclosed here, can be used to prepare transgenic plants and plants with altered traits. The specific transgenic plants listed below are produced from the sequences of the Sequence Listing, as noted. Table 4 provides exemplary polynucleotide and polypeptide sequences of the invention.

Example VIII Examples of Genes that Confer Significant Improvements to Plants

A number of genes and homologs that confer significant improvements to knockout or overexpressing plants were noted below. Experimental observations made with regard to specific genes whose expression was modified in overexpressing or knockout plants, and potential applications based on these observations, were also presented.

G8 (SEQ ID NO: 1)

Published Information

G8 corresponds to gene At2g28550 (AAD21489), and was described as RAP2.7 (Okamuro et al. (1997) Proc. Natl. Acad. Sci. 94:7076-7081).

Experimental Observations

The function of G8 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G8 caused alterations in plant development, the most consistent one being a delay in flowering time.

The individual plants showed a relatively strong phenotype and typically made 30-50 leaves (versus 10-12 the wild-type controls) prior to bolting, under 24-hour light. This phenotype was reproduced in some, but not all, of the T2 progeny plants from each one of the lines. Additionally, a further T2 population was found to flower later than wild type in 12-hour light conditions. Thus, late flowering was observed in both the T1 and T2 generations, and in different photoperiodic conditions.

It should also be noted that many 35S::G8 plants appeared smaller than controls, particularly at early stages. Accordingly, in the T2 lines used for physiological analyses it was observed that seedlings were smaller and showed reduced vigor when germinated on MS plates. However, not all 35S::G8 lines showed these effects.

G8 was ubiquitously expressed, at higher levels in rosette leaves.

Potential Applications

G8 or its equivalogs can be used to alter flowering time.

In general, a wide variety of applications exist for systems that either lengthen or shorten the time to flowering.

Most modern crop varieties were the result of extensive breeding programs. Many generations of backcrossing may be required to introduce desired traits. Systems that accelerate flowering can have valuable applications in such programs since they allow much faster generation times. Additionally, in some instances, a faster generation time can allow additional harvests of a crop to be made within a given growing season. With the advent of transformation systems for tree species such as oil palm and Eucalyptus, forest biotechnology is a growing area of interest. Acceleration of flowering can reduce generation times and make breeding programs feasible which would otherwise be impossible.

In species such as sugarbeet where the vegetative parts of the plants constitute the crop and the reproductive tissues were discarded, it is advantageous to delay or prevent flowering. Extending vegetative development can bring about large increases in yields. By regulating the expression of flowering-time controlling genes, using inducible promoters, flowering can be triggered as desired (for example, by application of a chemical inducer). This can allow, for example, flowering to be synchronized across a crop and facilitate more efficient harvesting. Such inducible systems can be used to tune the flowering of crop varieties to different latitudes. At present, species such as soybean and cotton were available as a series of maturity groups that were suitable for different latitudes on the basis of their flowering time (which is governed by day-length). A system in which flowering can be chemically controlled could allow a single high-yielding northern maturity group to be grown at any latitude. In southern regions such plants can be grown for longer, thereby increasing yields, before flowering was induced. In more northern areas, the induction can be used to ensure that the crop flowers prior to the first winter frosts. Currently, the existence of a series of maturity groups for different latitudes represents a major barrier to the introduction of new valuable traits. Any trait (e.g. disease resistance) has to be bred into each of the different maturity groups separately; a laborious and costly exercise. The availability of single strain, which can be grown at any latitude, could therefore greatly increase the potential for introducing new traits to crop species such as soybean and cotton.

For many crop species, high yielding winter-varieties can only be grown in temperate regions where the winter season is prolonged and cold enough to elicit a vernalization response. If the vernalization treatment can be compensated for by modulating the expression of certain transcription factors in crop plants, winter varieties of wheat, for instance, might then be grown in areas like Southern California which would otherwise be too warm to allow effective vernalization. Another application is in cherry (Prunus). Locally grown cherries are unavailable in the early Californian spring since the winters are too warm for vernalization to occur.

A further application exists in strawberry (Fragaria). Strawberry has a well-defined perennial cycle of flower initiation, dormancy, chilling, crop growth and runner production. In temperate European countries, the plants flower in early spring, and fruit is produced in May or June. Following fruiting, runners are generated that carry plantlets which take root. The plants then remain dormant all through the late summer and autumn. Flowering cannot be repeated until the following spring after the plants have received a winter cold treatment. A system that bypasses this vernalization requirement could permit a second autumn crop of strawberries to be harvested in addition to the spring crop.

G19 (SEQ ID NO: 3)

Published Information

G19 belongs to the EREBP subfamily of transcription factors and contains only one AP2 domain. G19 corresponds to the previously described gene RAP2.3 (Okamuro et al. (1997) Proc. Natl. Acad. Sci. 94:7076-7081). Close inspection of the Arabidopsis cDNA sequences of RAP2.3 (AF003096; Okamuro et al. (1997) supra), AtEBP (Y09942; Buttner et al. (1997) Proc. Natl. Acad. Sci. 94:5961-5966), and ATCADINP (Z37504) suggests that they may correspond to the same gene (Riechmann et al. (1998) Biol. Chem. 379:633-646). G19/RAP2.3 is ubiquitously expressed (Okamuro et al. (1997) supra). AtEBP was isolated by virtue of the protein-protein interaction between AtEBP and OBF4, a basic-region leucine zipper transcription factor (Buttner et al. (1997) supra). AtEBP expression levels in seedlings were increased after treatment with ethylene (ethephon) (Buttner et al. (1997) supra). AtEBP was found to bind to GCC-box containing sequences, like that of the PRB-1b promoter (Buttner et al. (1997) supra). It has been suggested that the interaction between AtEBP and OBF4 reflects cross-coupling between EREBP and bZIP transcription factors that might be important in regulating gene expression during the plant defense response (Buttner et al. (1997) supra).

Experimental Observations

Transgenic plants in which G19 is expressed under the control of the 35S promoter were morphologically similar to control plants. G19 is constitutively expressed in the different tissues examined; however G19 expression was significantly repressed by methyl jasmonate (MeJ) and induced by ACC (this latter result correlates with the previously described increase in G19 expression levels in seedlings after treatment with ethylene (ethephon); Buttner et al. (1997) supra). G19 was significantly induced upon infection by the fungal pathogen Erysiphe orontii. In addition, G19 overexpressing plants were more tolerant to infection with a moderate dose of Erysiphe orontii. G19 overexpressing plants were also tested for their tolerance to two other pathogens, the necrotrophic fungal pathogen Fusarium oxysporum and the bacterial pathogen Pseudomonas syringae; the transgenic plants were not found to have altered susceptibility to the pathogens.

Both the jasmonic acid and the ethylene signal transduction pathways were involved in the regulation of the defense response and the wound response, and the two pathways have been found to interact synergistically. The regulation of G19 expression by both hormones, its induction upon Erysiphe orontii infection, as well as the preliminary data indicating that increased tolerance to that pathogen was conferred by G19 overexpression, suggest that G19 plays a role in the control of the defense and/or wound response. It would be of interest to test G19 overexpressing plants in insect-plant interaction experiments. The increase in tolerance to Erysiphe orontii that is conferred by G19 overexpression can be tested using other races of the pathogen. It would also be of interest to test other pathogens in addition to Erysiphe orontii, Fusarium oxysporum, and Pseudomonas syringae.

Since G19 was expressed at significant levels in a constitutive fashion, similar experiments to those described here can be performed with G19 knockout mutant plants to further elucidate the function of this gene.

Potential Applications

G19 or its equivalogs can be used to manipulate the plant defense- wound- or insect-response, as well as the jasmonic acid and ethylene signal transduction pathways themselves.

G22 (SEQ ID NO: 5)

Published Information

G22 was identified in the sequence of BAC T13E15 (gene T13E15.5) by The Institute of Genomic Research (TIGR) as a “TINY transcription factor isolog”. G22 belongs to the EREBP subfamily and contains only one AP2 domain, and phylogenetic analyses place G22 relatively close to other EREBP subfamily genes, such as, TINY and ATDL4400C (Riechmann et al. (1998) Biol. Chem. 379:633-646).

Experimental Observations

G22 was constitutively expressed at medium levels. There appeared to be no phenotypic alteration on plant morphology upon G22 overexpression. Plants ectopically overexpressing G22 were more tolerant to high NaCl containing media in a root growth assay compared with wild-type controls.

Potential Applications

G22 or its equivalogs can be used to increase plant tolerance to soil salinity during germination, at the seedling stage, or throughout the plant life cycle. Salt tolerance is a particularly desirable phenotype during the germination stage of a crop plant, which would impact survivability and yield.

G24 (SEQ ID NO: 7)

Published Information

G24 corresponds to gene At2g23340 (AAB87098).

Experimental Observations

The function of G24 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G24 caused alterations in plant growth and development. Most notably, 35S::G24 seedlings often developed black necrotic tissue patches on cotyledons and leaves, and many died at that stage. Some 35S::G24 seedlings exhibited a weaker phenotype, and although necrotic patches were visible on the cotyledons, they did not die. These seedlings developed into plants that were usually small, slow growing, and poorly fertile in comparison to wild-type controls. The leaves of older 35S::G24 plants were also observed to become yellow and senesce prematurely compared with wild type. For those lines that could be assayed in biochemical and physiological assays, no differences were observed with respect to wild-type controls.

G24 was ubiquitously expressed, at apparently lower levels in germinating seedlings.

The AP2 domain of G24 is nearly identical to that of other Arabidopsis EREBP proteins, such as G12, G1379, and G1277.

Potential Applications

G24 or its equivalogs can be used to trigger cell death and influence or control processes in which cell death plays a role. G24 can be used to block pathogen infection by triggering it in infected cells and blocking spread of the disease.

G28 (SEQ ID NO: 9)

Published Information

G28 corresponds to AtERF1 (GenBank accession number AB008103) (Fujimoto et al. (2000) Plant Cell 12:393-404). G28 appears as gene AT4 g17500 in the annotated sequence of Arabidopsis chromosome 4 (AL161546.2).

AtERF1 has been shown to have GCC-box binding activity [some defense-related genes that were induced by ethylene were found to contain a short cis-acting element known as the GCC-box: AGCCGCC (Ohme et al. (1990) Plant Mol. Biol. 15:941-946)]. Using transient assays in Arabidopsis leaves, AtERF1 was found to be able to act as a GCC-box sequence specific transactivator (Fujimoto et al. (2000) supra).

AtERF1 expression has been described to be induced by ethylene (two- to three-fold increase in AtERF1 transcript levels 12 h after ethylene treatment) (Fujimoto et al. (2000) supra). In the ein2 mutant, the expression of AtERF1 was not induced by ethylene, suggesting that the ethylene induction of AtERF1 is regulated under the ethylene signaling pathway (Fujimoto et al. (2000) supra). AtERF1 expression was also induced by wounding, but not by other abiotic stresses (such as cold, salinity, or drought) (Fujimoto et al. (2000) supra).

It has been suggested that AtERFs, in general, may act as transcription factors for stress-responsive genes, and that the GCC-box may act as a cis-regulatory element for biotic and abiotic stress signal transduction in addition to its role as an ethylene responsive element (ERE) (Fujimoto et al. (2000) supra), but there is no data available on the physiological functions of AtERF1.

Experimental Observations

The function of G28 was analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter. G28 overexpressing lines were more tolerant to infection with a moderate dose of the fungal pathogen Erysiphe orontii. G28 overexpression did not seem to have detrimental effects on plant growth or vigor, since plants from most of the lines were morphologically wild-type. In addition, no difference was detected between those lines and the corresponding wild-type controls in all the biochemical assays that were performed. G28 was ubiquitously expressed.

G28 overexpressing lines were also more tolerant to Sclerotinia sclerotiorum and Botrytis cinerea. In a repeat experiment using individual lines, all three lines analyzed showed tolerance to S. sclerotiorum, and two of the three lines tested were more tolerant to B. cinerea.

Potential Applications

G28 transgenic plants had an altered response to fungal pathogens, in that those plants were more tolerant to the pathogens. Therefore, G28 or its equivalogs can be used to manipulate the defense response in order to generate pathogen-resistant plants.

G47 (SEQ ID NO: 11)

Published Information

G47 corresponds to gene T22J18.2 (AAC25505).

Experimental Observations

The function of G47 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G47 resulted in a variety of morphological and physiological phenotypic alterations.

35S::G47 plants showed enhanced tolerance to osmotic stress. In a root growth assay on PEG containing media, G47 overexpressing transgenic seedlings were larger and had more root growth compared with the wild-type controls. G47 expression levels can be altered by environmental conditions, in particular reduced by salt and osmotic stresses.

Overexpression of G47 also produced a substantial delay in flowering time and caused a marked change in shoot architecture. 35S::G47 transformants were small at early stages and switched to flowering more than a week later than wild-type controls (continuous light conditions). The inflorescences from these plants appeared thick and fleshy, had reduced apical dominance, and exhibited reduced internode elongation leading to a short compact stature. The branching pattern of the stems also appeared abnormal, with the primary shoot becoming “kinked” at each cofluorescence node. Additionally, the plants showed reduced fertility and formed rather small siliques that were borne on short pedicels and held vertically, close against the stem.

Additional alterations were detected in the inflorescence stems of 35S::G47 plants. Stem sections from T2-21 and T2-24 plants were of wider diameter, and had large irregular vascular bundles containing a much greater number of xylem vessels than wild type. Furthermore, some of the xylem vessels within the bundles appeared narrow and were possibly more lignified than were those of controls.

G47 was expressed at higher levels in rosette leaves, and transcripts were detected in other tissues (flower, embryo, silique, and germinating seedling), but not in roots.

Potential Applications

G47 or its equivalogs can be used to manipulate flowering time, to modify plant architecture and stem structure (including development of vascular tissues and lignin content) and to improve plant performance under osmotic stress.

Transcription factor equivalogs that modulate lignin content can be valuable. This modulation can allow the quality of wood used for furniture or construction to be improved. Lignin is energy rich; increasing lignin composition is valuable in raising the energy content of wood used for fuel. Conversely, the pulp and paper industries seek wood with a reduced lignin content. Currently, lignin must be removed in a costly process that involves the use of many polluting chemicals. Consequently, lignin is a serious barrier to efficient pulp and paper production. In addition to forest biotechnology applications, changing lignin content can increase the palatability of various fruits and vegetables.

G156 (SEQ ID NO: 13)

Published Information

G156 corresponds to gene MKD15.12 (GenBank accession number BAB11181.1). G156 has also been described as AGL32 (Alvarez-Buylla et al. (2000) Proc. Natl. Acad. Sci. 97:5328-5333). Phylogenetic analyses of the Arabidopsis MADS box gene family indicate that G156/AGL32 is a Type II MADS-box gene, but it does not belong to any of the well-characterized Type II MADS gene clades (Alvarez-Buylla et al. 2000 supra).

Experimental Observations

The complete cDNA sequence of G156 was determined. The function of this gene was analyzed using both transgenic plants in which G156 was expressed under the control of the 35S promoter and a line homozygous for a T-DNA insertion in the gene. The T-DNA insertion lies in the second intron, and was expected to result in a strong loss-of-function or null mutation.

G156 knockout mutant plants produced yellow seed that showed more variation in shape than wild type, implying a function (direct or indirect) for G156 in seed development. G156 mutant plants were otherwise normal at all other developmental stages. Expression of G156 was determined to be specific to floral tissues. Although expression was detected by RT-PCR in flowers, siliques, and embryos, it could well be that G156 was specifically expressed in embryo/seed during development, in light of the many MADS box genes that have been shown to be expressed in specific floral organs or cell types, and of the G156 knockout mutant phenotype. In situ RNA hybridization experiments will determine more precisely G156 expression pattern.

The coloration phenotype of the G156 knockout mutant seed resembles that of ttg1 and the transparent testa mutants. TTG1, which is localized in Chromosome 5, but approximately 0.5 Mb away from the clone that contains G156 (MKD15), codes for a WD40 repeat protein (Walker et al. (1999) Plant Cell 11:1337-1350). The transparent testa (tt) loci were identified in screens for mutations that result in yellow or pale brown seeds (Koornneef (1990) Arabidopsis Inf. Ser. 27:1-4). Many of the “TT” genes have been mapped, and several of them have been cloned and shown to be involved in the anthocyanin pathway (Debeaujon et al. (2001) Plant Cell 13:853-872)

None of the TT genes corresponds to G156. TT3, TT4, TT5, and TT7 code for dihydroflavol 4-reductase, chalcone synthase, chalcone flavanone isomerase, and flavonoid 3′-hydroxylase, respectively (Shirley et al. (1992) Plant Cell 4:333-347; Shirley et al. (1995) Plant J. 8:659-671). TT12 encodes a multidrug secondary transporter-like protein required for flavonoid sequestration in vacuoles of the seed coat endothelium (Debeaujon et al. (2001) supra). TT6 and TT9 map on Chromosome 3, and TT1 maps on Chromosome 1. TT2 and TT10 map on Chromosome 5, but far away from the position of G156 (Shirley et al. (1995) supra). TT8 has also been cloned and shown to encode a transcription factor of the basic helix-loop-helix class (Nesi et al. (2000) Plant Cell 12:1863-1878), providing further evidence for the regulation of the anthocyanin pathway at the transcriptional level.

The similarity of the G156 knockout and tt seed coloration phenotypes, and the involvement of at least some of the TT genes in the anthocyanin pathway, suggested that G156 is involved in its regulation.

In addition to the seed coloration phenotype, the G156 knockout mutant showed a significant increase in the percentage of seed 18:1 fatty acids.

G156 overexpressing plants showed a variety of morphological alterations, largely uninformative. The most severely affected transformants were extremely dwarfed, had aberrant branching, and sometimes possessed terminal flowers. These phenotypic alterations were frequently observed when MADS box genes that were involved in flower development were overexpressed in Arabidopsis (for instance, AG, AP1, and AP3+PI; Mizukami et al. (1992) Cell 71:119-131; Mandel et al. (1995) Nature 377:522-524; Krizek et al. (1996) Development 122:11-22).

Both G156 knockout mutant plants and G156 overexpressing lines behaved like the wild-type controls in the physiological assays performed.

Potential Applications

G156 or its equivalogs can be used to manipulate the anthocyanin biosynthetic pathway, such as for altering seed coloration. In addition, the promoter of G156 may be used to confer seed-specific expression to genes of interest.

G157 (SEQ ID NO: 15)

Published Information

G157 was first identified in the sequence of BAC F22K20 (GenBank accession number AC002291; gene F22K20.15).

Experimental Observations

G157 was recognized as a gene highly related to Arabidopsis FLOWERING LOCUS C (FLC; Michaels et al. (1999) Plant Cell 11:949-956; Sheldon et al. (1999) Plant Cell 11:445-458). FLC acts as a repressor of flowering. Late flowering vernalization responsive ecotypes and mutants have high steady state levels of FLC transcript, which decrease during the promotion of flowering by vernalization. FLC therefore has a central role in regulating the response to vernalization (Michaels (1999) supra; Sheldon et al. (1999) supra; Sheldon et al. (2000) Proc. Natl. Acad. Sci. 97:3753-3758).

The function of G157 was studied using transgenic plants in which this gene was expressed under the control of the 35S promoter. Over-expression of G157 modifies flowering time, and it appears to do so in a quantitative manner: a modest level of over-expression triggers early flowering, whereas a larger increase delays flowering. G157 over-expression promoted flowering in the Arabidopsis late-flowering vernalization-dependent ecotypes Stockholm and Pitztal.

In contrast to FLC, G157 transcript levels showed no correlation with the vernalization response, and over-expression of G157 did not influence FLC transcript levels. Thus, G157 likely acts downstream or independently of FLC transcription. In addition, a cluster of four additional FLC-like and G157-like genes were identified, raising the possibility that a whole sub-group of proteins within the MADS family regulates flowering time.

G157 overexpressing plants did not show any other morphological, physiological, or biochemical alteration in the assays that were performed. Overexpression of G157 was not observed to have deleterious effects: 35S::G157 plants were healthy and attained a wild-type stature when mature.

For many crops, high yielding winter strains can only be grown in regions where the growing season is sufficiently cold and prolonged to elicit vernalization. A system that could trigger flowering at higher temperatures would greatly expand the acreage over which winter varieties can be cultivated. The finding that G157 overexpression caused early flowering in Arabidopsis Stockholm and Pitztal plants, indicated that the gene can overcome the high level of FRIGIDA and FLC activity present in those late-ecotypes. That the effects were similar to those caused by vernalization implied that G157 might be applicable to winter strains of crop species. To date, a substantial number of genes have been found to promote flowering. Many, however, including those encoding the transcription factors, APETALA1, LEAFY, and CONSTANS, produce extreme dwarfing and/or shoot termination when over-expressed. Overexpression of G157 was not observed to have deleterious effects. 35S::G157 Arabidopsis plants were healthy and attained a wild-type stature when mature. Irrespective of the mode of G157 action, and whether its true biological role is as an activator or a repressor of flowering, the results suggested that G157 may produce either early or late flowering, according to the level of over-expression.

G162 (SEQ ID NO: 17)

Published Information

G162 corresponds to gene At2g34440 (AAC26702), and it has also been referred to as AGL29.

Experimental Observations

The function of G162 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G162 plants were wild-type in morphology and development. Overexpression of G162 resulted in a significant increase in oil content in seeds, as measured by NIR.

Potential Applications

G162 or its equivalogs may be used to increase seed oil content and manipulate seed protein content in crop plants.

G175 (SEQ ID NO: 19)

Published Information

G175 was identified in the sequence of P1 clone M3E9 (Gene AT4g26440/M3E9.130; GenBank accession number CAB79499).

Experimental Observations

The complete cDNA sequence of G175 was determined. The function of this gene was studied using transgenic plants in which G175 was expressed under the control of the 35S promoter. 35S::G175 plants were more tolerant to osmotic stress conditions (better germination in NaCl- and sucrose-containing media). The plants were otherwise wild-type in morphology and development. Whereas some phenotypic changes were detected in the biochemical assays that were performed, these were not observed in more than one line.

G175 appeared to be specifically expressed in floral tissues, and also appeared to be induced elsewhere by heat and salt stress.

Potential Applications

G175 or its equivalogs can be used to increase germination under adverse osmotic stress conditions, which could impact survivability and yield. The promoter of G175 may be used to drive flower specific expression.

G180 (SEQ ID NO: 21)

Published Information

G180 was identified in the sequence of BAC F16B22 (GenBank accession number AC003672).

Experimental Observations

The complete sequence of G180 was determined. G180 was not annotated in the sequence of Arabidopsis thaliana chromosome II section 239 of 255 of the complete sequence (AC003672.2), where it resides between At2g44740 and At2g44750.

The function of G180 was analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter.

G180 overexpressing plants were early flowering, but did not exhibit other major developmental alterations. A number of Arabidopsis genes have already been shown to accelerate flowering when constitutively expressed. These include LEAFY, APETALA1 and CONSTANS. In these cases, however, the early flowering plants showed undesirable side effects such as extreme dwarfing, infertility, or premature termination of shoot meristem growth (Mandel et al. (1995) Nature 377:522-524; Weigel et al. (1995) 377: 495-500; Simon et al. (1996) Nature 384:59-62). It appeared that G180 induced flowering without these toxic pleiotropic effects.

G180 overexpressing lines also showed a decrease in seed oil content. That decrease was accompanied increased seed protein content in one of the three lines analyzed.

Potential Applications

G180 overexpression appeared to alter flowering time by accelerating the transition from vegetative to reproductive state. Therefore, G180 or its equivalogs may be used to manipulate flowering time in plants. In addition, G180 or its equivalogs can also have utility in modifying seed traits, particularly in modifying seed oil and protein levels in crop plants.

G183 (SEQ ID NO: 23)

Published Information

G183 corresponds to gene F20N2.3 (AAF79511).

Experimental Observations

The function of G183 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G183 resulted in an a reduction of the time to flowering.

Under continuous light conditions, 35S::G183 plants formed flower buds approximately 2-4 days earlier than wild-type control plants. Such a phenotype was seen in two separate plantings and in each of two independent T2 lines. Overexpression of G183 also resulted in seedlings with an altered response to light. In a germination assay conducted in darkness, G183 seedlings failed to show an etiolation response. However, the phenotype was severe in seedlings from one line where overexpression of the transgene resulted in reduced hypocotyl elongation and open, greenish cotyledons, but this line did not show alterations in flowering time in the T2 generation.

In addition to the effects on flowering time, 35S::G183 transformants were generally small, produced rather thin inflorescences, and had a low seed yield compared with wild type. Such effects were particularly apparent in some of the T1 plants. It should also be noted that the transformation rate attained with this transgene was relatively low, suggesting that G183 might have lethal effects at high dosages. Overexpression of G183 did not result in any biochemical phenotypic alteration.

According to the results obtained in the RT-PCR experiments, G183 was specifically expressed in flower, embryo, and silique tissues. It should be noted, however, that there have already been cases described of Arabidopsis transcription factor genes that were specifically expressed in flower-derived tissues but that can affect flowering time when their expression pattern is modified, including a homeobox gene long considered representing a true flowering time locus, FWA.

Potential Applications

G183 or its equivalogs may be used to modify flowering time and light response.

G183 or its equivalogs may alter a plant's light response and thus modify growth or development, for example, photomorphogenesis in poor light, or accelerating flowering time in response to various light intensities, quality or duration to which a non-transformed plant would not similarly respond, and increased planting densities with subsequent yield enhancement.

G188 (SEQ ID NO: 25)

Published Information

G188 corresponds to gene MXC20.3, first identified in the sequence of clone MXC20 (released by the Arabidopsis Genome Initiative; GenBank accession number AB009055).

Experimental Observations

The annotation of G188 in BAC AB009055 was experimentally confirmed. G188 was expressed in all tissues and under all conditions examined.

A line homozygous for a T-DNA insertion in G188 was initially used to characterize the function of this gene. In such line, the T-DNA insertion in G188 was localized in the second intron of the gene, located in the middle of the conserved WRKY box. Such insertion would result in a null mutation (unless the large fragment of exogenous sequence is perfectly spliced out from the transcribed G188 pre-mRNA). G188 mutant plants displayed several phenotypic alterations in physiological assays. G188 knockout mutant seed germinated better than wild-type controls under several kinds of osmotic stress. G188 knockout plants also showed higher susceptibility to the necrotroph fungal pathogen Fusarium oxysporum compared with control plants (more disease spread after infection). No significant morphological changes were observed in G188 knockout plants.

The function of G188 was subsequently analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. G188 overexpressing plants were morphologically wild-type, and indistinguishable from the corresponding controls in all physiological and biochemical assays that were performed. Overexpression of G188 did not increase resistance to Fusarium oxysporum.

Further experiments to characterize G188 function can include testing the plant (knockout or overexpressor) to different doses of the pathogen Fusarium oxysporum, as well as more sophisticated gene expression profiling experiments.

Potential Applications

G188 or its equivalogs can be used to enhance seed germination under adverse osmotic conditions. G188 appears to be involved in the plant's response to Fusarium oxysporum and thus it may be used to manipulate such responses.

G189 (SEQ ID NO ID NO: 27)

Published Information

G189 was identified in the sequence of BAC clone T20D16 (gene At2g23320/T20D16.5, GenBank accession number AAB87100).

Experimental Observations

The function of G189 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. T1 G189 overexpressing plants showed leaves of larger area than wild type. This phenotype, which was observed in two different T1 plantings, became more apparent at late vegetative development. T2 plants were morphologically wild-type. In wild-type plants, G189 was constitutively expressed.

G189 overexpressing plants were wild-type in all the physiological analyses performed.

Potential Applications

G189 or its equivalogs can be used to increase plant biomass. Large size is useful in crops where the vegetative portion of the plant is the marketable portion since vegetative growth often stops when plants make the transition to flowering.

G192 (SEQ ID NO: 29)

Published Information

G192 corresponds to gene A_IG002N01.6, first identified in the sequence of BAC clone A_IG002N01 (released by the Arabidopsis Genome Initiative; GenBank accession number AF007269).

Experimental Observations

The annotation of G192 in BAC AF007269 was experimentally confirmed. G192 was expressed in all plant tissues and under all conditions examined. Its expression was induced upon infection by Fusarium.

The function of G192 was analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter. G192 overexpressors were late flowering under 12 hour light and had more leaves than control plants. This phenotype was manifested in the three T2 lines analyzed. In addition, one line showed a decrease in seed oil content. No other differences between G192 overexpressing lines and control plants were noted in the assays performed.

A decrease in seed oil observed previously in one transgenic line was replicated in an independent experiment.

Potential Applications

G192 overexpression delayed flowering. A wide variety of applications exist for genes or their equivalogs that either lengthen or shorten the time to flowering, or for systems of inducible flowering time control. In particular, in species where the vegetative parts of the plants constitute the crop and the reproductive tissues were discarded, it would be advantageous to delay or prevent flowering. Extending vegetative development may bring about large increases in yields.

G192 or its equivalogs can be used to manipulate seed oil content, which might be of nutritional value.

G196 (SEQ ID NO: 31)

Published Information

G196 corresponds to gene At2g34830 (AAC12823).

Experimental Observations

The function of G196 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G196 plants show more tolerance to salt stress in a germination assay. Overexpression of G196 also produced a range of effects on plant morphology including a reduction in overall size, lowered fertility and changes in leaf shape. T1 seedlings were typically small, often had abnormal shaped cotyledons, and the rosette leaves produced by these plants were often undersized, contorted and darker green compared with wild type. Later in development, during the reproductive stage, the plants formed thin inflorescences bearing poorly fertile flowers with underdeveloped organs. 35S::G196 primary transformants were obtained at a relatively low frequency, suggesting that the gene might have lethal effects if overexpressed at very high levels.

35S::G196 plants were wild-type in the biochemical analyses that were performed. G196 was ubiquitously expressed (and different levels among the various tissues).

Potential Applications

G196 or its equivalogs may be used to improve plant performance under conditions of salt stress. Evaporation from the soil surface causes upward water movement and salt accumulation in the upper soil layer where the seeds were placed. Thus, germination normally takes place at a salt concentration that is higher than the mean salt concentration in the whole soil profile. Increased salt tolerance during the germination stage of a crop plant may impact survivability and yield.

G211 (SEQ ID NO: 33)

Published Information

G211 corresponds to Atmyb5 (U26935; Li et al. (1996) FEBS Lett 379:117-121). Arabidopsis plants transgenic for a chimeric Atmyb5 promoter/GUS gene expressed the enzyme in developing leaf trichomes, stipules, epidermal cells on the margins of young rosette and cauline leaves, and in immature seeds. In immature seeds, Atmyb5 expression occurs between fertilization and the 16 cell stage of embryo development and persists beyond the heart stage.

Experimental Observations

The function of G211 was investigated using a homozygous mutant line in which a T-DNA was inserted into the coding region of the gene as well as using transgenic lines in which G211 is expressed under the control of the 35S promoter. The phenotype of the G211 knockout mutant plants was wild-type in all respects. Overexpression of G211, however, had marked effects on leaf and inflorescence development. 35S::G211 plants were generally small, slow developing, and produced rounded, slightly serrated leaves, with very short petioles. Additionally these plants were dark green in coloration, and in some cases, appeared to have reduced trichome density. Following the switch to reproductive growth, 35S::G211 inflorescences had short internodes and showed a general reduction in apical dominance, leading to a bushy appearance. In many cases, due to the small size, seed yield was reduced compared with wild-type controls. These effects were highly penetrant and were apparent in the majority of T1 lines and, to some extent, in each of the three T2 populations. An increase in leaf xylose in two lines was also observed in the T2 35S::G211 transgenics.

As determined by RT-PCR, expression of G211 was found primarily in embryos and siliques. G211 expression in leaf tissue was unaffected by any environmental stress-related condition tested.

Potential Applications

G211 overexpression resulted in plants with altered leaf insoluble sugar content. Transcription factors such as G211 or their equivalogs that alter plant cell wall composition have several potential applications including altering food digestibility, plant tensile strength, wood quality, pathogen resistance and in pulp production.

In particular, hemicellulose is not desirable in paper pulps because of its lack of strength compared with cellulose. Thus, modulating the amounts of cellulose vs. hemicellulose in the plant cell wall is desirable for the paper/lumber industry. Increasing the insoluble carbohydrate content in various fruits, vegetables, and other edible consumer products will result in enhanced fiber content. Increased fiber content would not only provide health benefits in food products, but might also increase digestibility of forage crops. In addition, the hemicellulose and pectin content of fruits and berries affects the quality of jam and catsup made from them. Changes in hemicellulose and pectin content could result in a superior consumer product.

G214 (SEQ ID NO ID NO: 35)

Published Information

G214 (CCA1) was published by Wang et al. (1997) Plant Cell 9: 491-507. CCA1 is involved in phytochrome induction of CAB genes. The transcript is transiently induced by phytochrome and oscillates with a circadian rhythm. It feedback-regulates its own expression at the transcriptional level. Overexpressing CCA1 abolished circadian rhythm of several genes and results in plants that were late flowering, and have elongated hypocotyls.

Experimental Observations

G214 overexpressing lines were late bolting, show larger biomass (increased leaf number and size), and were darker green in vegetative and reproductive tissues due to a higher chlorophyll content in the later stages of development. In these later stages, the overexpressors also have higher insoluble sugar, leaf fatty acid, and carotenoid content per unit area. Line #11 also showed a significant, repeatable increase in lutein levels in seeds. Microarray data was consistent with the morphological and biochemical data in that the genes that were highly induced included chloroplast localized enzymes, and light regulated genes such as Rubisco, carbonic anhydrase, and the photosystem 1 reaction center subunit precursor. A chlorophyll biosynthetic enzyme was also highly induced, consistent with the dark green color of the adult leaves and perhaps a higher photosynthetic rate. A measurement of leaf fatty acid in the older overexpressors suggested that the overall levels were higher than wild-type levels (except for the percent composition of 16:3 in line #11). Percent composition of 16:1 and 16:3 fatty acids (found primarily in plastids) is similar to wild type arguing against an increase in chloroplast number as an explanation for increase chlorophyll content in the leaves. Three G214-overexpressing lines were sensitive to germination on high glucose showing less cotyledon expansion and hypocotyl elongation suggesting the late bolting and dark green phenotype could be tied into carbon sensing which has been shown to regulate phytochrome A signaling (Dijkwel et al. (1997) Plant Cell 9:583-595; Van Oosten et al. (1997) Plant J. 12:1011-1020). Sugars are key regulatory molecules that affect diverse processes in higher plants including germination, growth, flowering, senescence, sugar metabolism and photosynthesis. Glucose-specific hexose-sensing has also been described in plants and implicated in cell division and the repression of famine genes (photosynthetic or glyoxylate cycles).

Potential Applications

Potential utilities of this gene or its equivalogs include increasing chlorophyll content allowing more growth and productivity in conditions of low light. With a potentially higher photosynthetic rate, fruits can have higher sugar content. Increased carotenoid content may be used as a nutraceutical to produce foods with greater antioxidant capability. G214 or its equivalogs can also be used to manipulate seed composition, which is very important for the nutritional value and production of various food products.

G214 overexpression delayed flowering time in transgenic plants, and thus this gene or its equivalogs would be useful in modifying flowering time. In a sizeable number of species, for example, root crops, where the vegetative parts of the plants constitute the crop and the reproductive tissues were discarded, it is advantageous to identify and incorporate transcription factor genes that delay or prevent flowering in order to prevent resources being diverted into reproductive development. Extending vegetative development can thus bring about large increases in yields.

G226 (SEQ ID NO: 37)

Published Information

G226 was identified from the Arabidopsis BAC sequence, AC002338, based on its sequence similarity within the conserved domain to other Myb family members in Arabidopsis. To date, there is no published information regarding the function of this gene.

Experimental Observations

The function of G226 was analyzed through its ectopic overexpression in plants. G226 overexpressors were more tolerant to low nitrogen and high salt stress. They showed more root growth and possibly more root hairs under conditions of nitrogen limitation compared with wild-type controls. Many plants were glabrous and lacked anthocyanin production when under stress such as growth conditions of low nitrogen and high salt. Several G226 overexpressors were glabrous and produce less anthocyanin under stress; these effects might be due to binding site competition with other Myb family transcription factors involved in these functions and not directly related to the primary function of this gene.

Results from the biochemical analysis of G226 overexpressors suggested that one line had higher amounts of seed protein, which could have been a result of increased nitrogen uptake by these plants.

A microarray experiment was done on a separate G226 overexpressing line. The G226 sequence itself was overexpressed 16-fold above wild type, however, very few changes in other gene expression were observed in this line. On the array, a chlorate/nitrate transporter DNA sequence was induced 2.7-fold over wild type, which could explain the low nitrogen tolerant phenotype of the plants and the increased amounts of seed protein in one of the lines. The same DNA sequence was present several times on the array and in all cases the DNA sequence showed induction, adding more validity to the data. Five other genes/DNA sequences induced but had unknown function. A methyltransferase, a pollen-specific protein, and a zinc binding peroxisomal membrane protein encoding sequences were also induced, however their role in regard to the phenotype of the plants is not known.

Potential Applications

The utilities of a gene or its equivalogs conferring tolerance to conditions of low nitrogen include: (1) Cost savings to the farmer by reducing the amounts of fertilizer needed; (2) Environmental benefits of reduced fertilizer runoff, (3) Improved yield and stress tolerance. In addition, G226 can be used to increase seed protein amounts and/or composition, which may impact yield as well as the nutritional value and production of various food products.

G226 or its equivalogs can be used to alter trichome number and distribution in plants. Trichome glands on the surface of many higher plants produce and secrete exudates, which give protection from the elements and pests such as insects, microbes and herbivores. These exudates may physically immobilize insects and spores, may be insecticidal or antimicrobial or they may allergens or irritants to protect against herbivores. It has also been suggested that trichomes may decrease transpiration by decreasing leaf surface airflow, and by exuding chemicals that protect the leaf from the sun.

G241 (SEQ ID NO: 39)

Published Information

G241 is equivalent to Y19 (X90384), a putative light regulated Myb that was identified by Quaedvlieg et al. (1996) Plant Mol. Biol. 32:987-993. The Myb Consortium renamed this gene MYB15 and found that it was constitutively expressed at a low level with expression higher in etiolated seedlings (Kranz et al. (1998) Plant J. 16:263-276).

Experimental Observations

The function of G241 was analyzed through its ectopic overexpression in plants as well as through the analysis of a line homozygous for a knockout mutation in G241. The knockout mutant plants were wild-type in all assays performed. G241 overexpressors had a glucose germination phenotype suggesting these plants could be involved in glucose-specific sugar sensing.

Results from the biochemical analysis of G241 knockouts showed that a lower amount of seed oil and an increase in seed protein.

RT-PCR analysis of the endogenous levels of G241 showed the gene is expressed in all tissue types tested.

Results from an array experiment using a G241 overexpressor line were consistent with expression in seeds. Several gene sequences were induced that could be involved in osmotic stress tolerance or desiccation tolerance, which are important for germinating seeds. In this experiment, the G241 DNA sequence itself was induced 38-fold. Many of the induced genes were transcription factors with unknown function. Both CBF1 and CBF2 (involved in freezing tolerance) were up-regulated. As mentioned above, several genes indicative of osmotic stress tolerance were also up-regulated. These same gene sequences were up-regulated on arrays of plants treated with mannitol as an osmotic stress, in a CBF2 overexpressor, and in cold-acclimated plants. A glucose transporter sequence was also up-regulated, however, this gene sequence is not up-regulated in any of the other arrays mentioned above. The phenotype of the overexpressor was reduced seedling growth on high glucose. It is possible that the plants were taking up more glucose. In such a scenario, the gene is not likely to be involved in sugar sensing but rather the high glucose condition is inhibiting their growth. The G241 overexpressors were tested for osmotic stress tolerance using mannitol. It is possible the glucose transporter is increasing mannitol uptake and increasing its toxicity to the plant as well. Polyethylene glycol (PEG) is an alternative osmoticum that can be tested at various concentrations.

Potential Applications

One potential utility of this gene or its equivalogs can be to engineer plants that are tolerant to stress. This can greatly impact yield. Alternatively, if this gene is involved in sugar sensing, the potential utility of a gene involved in glucose-specific sugar sensing is to alter energy balance, photosynthetic rate, biomass production, and senescence. Sugars are key regulatory molecules that affect diverse processes in higher plants including germination, growth, stress responses, flowering, senescence, sugar metabolism and photosynthesis. Glucose-specific hexose-sensing has been described in plants and implicated in cell division, and repression of famine genes (photosynthetic or glyoxylate cycles). This gene may also be used to alter oil and protein production in seeds, which may be very important for the nutritional quality and caloric content of foods.

G248 (SEQ ID NO: 41)

Published Information

G248 was identified at Mendel Biotechnology. Kranz et al. ((1998) Plant J. 16:263-276) published a cDNA sequence corresponding to G248, naming it MYB22.

Experimental Observations

The function of G248 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. The phenotype of these transgenic plants was wild-type with respect to their morphology. However, overexpression of G248 in Arabidopsis was found to confer greater sensitivity to disease, particularly following infection by Botrytis cinerea. All three lines show the susceptible phenotype.

As determined by RT-PCR, G248 appears to be expressed at low levels in embryo and silique tissue. No expression was detected in other tissues. G248 appears to be induced in response to salicylic acid (SA) treatment. It is well know that both synergistic and antagonistic crosstalk between growth regulator controlled defense pathways occurs in response to disease.

Potential Applications

Since G248 transgenic plants had an altered response to the fungal pathogen Botrytis cinerea, G248 or its equivalogs can be used to manipulate the defense response in order to generate pathogen-resistant plants.

G254 (SEQ ID NO: 43)

Published Information

G254 was identified from the Arabidopsis BAC sequence, AF007269, based on its sequence similarity within the conserved Myb domain to other Myb family members in Arabidopsis.

Experimental Observations

The function of G254 was analyzed through the ectopic overexpression of the gene in plants. Overexpression of G254 resulted in a reduction of germination and reduced seedling growth on glucose containing media. G254 may be involved in sugar sensing.

RT-PCR analysis of the endogenous levels of G254 indicated that this gene was expressed in all tissues tested. A cDNA microarray experiment supported the tissue distribution data by RT-PCR. There was no induction of G254 above its basal level in response to environmental stress treatments. G254 was constitutively expressed.

Potential Applications

The potential utility of G254 or its equivalogs is to alter source-sink relationships in the plant. Sugars are key regulatory molecules that affect diverse processes in higher plants including germination, growth, flowering, senescence, sugar metabolism, and photosynthesis. Sucrose is the major transport form of photosynthate and its flux through cells has been shown to affect gene expression and alter storage compound accumulation in seeds (source-sink relationships). The potential utilities of a gene involved in glucose-specific sugar sensing are to alter energy balance, photosynthetic rate, carbohydrate accumulation, biomass production, source-sink relationships, and senescence. Glucose-specific hexose-sensing has been described in plants and implicated in cell division and the repression of ‘famine’ genes (photosynthetic or glyoxylate cycles).

G256 (SEQ ID NO ID NO: 45)

Published Information

G256 is equivalent to Y13, a gene that was identified by Quaedvlieg et al. ((1996) Plant Mol. Biol. 32:987-093) as being induced in etiolated seedlings one hour after being exposed to light. The Myb consortium has renamed this gene MYB31. Quaedvlieg et al. (1996, supra) found a low level of expression in stem and silique tissue with no induction in etiolated seedlings after being exposed to light. However, there was also a slight induction of G256 following cold treatment.

Experimental Observations

The function of G256 was analyzed through its ectopic overexpression in plants. G256 overexpressors had enhanced seedling vigor during cold germination. These overexpressing lines were more tolerant to chilling conditions compared to wild-type controls, as seen in 12-day-old seedlings that were transferred to cold temperatures (8° C.).

There was no difference in germination rate under normal growth conditions. The chilling tolerant phenotype is most noticeable with respect to enhanced root growth although the cotyledons show less anthocyanin production than wild-type controls.

Plants overexpressing G256 were also small and early bolting. In the T2, one line lacked the waxy surface on the bolts. Three lines were tolerant to cold germination and therefore co-suppression was not a likely cause of the morphological change observed in one line. An array experiment was performed on this G256 overexpressing line. The gene itself was induced 3.5-fold over wild-type levels. Very few additional gene sequences were significantly induced in response to G256 overexpression. Induced genes included four gene sequences of unknown function, a sugar carrier sequence, a cell wall degrading enzyme (BGL2) sequence, pectinesterase sequence, and a proteasome subunit protein sequence. Expression of gene sequences such as allene oxidase sequence (which could mean down-regulation of the associated jasmonate synthesis pathway), and endochitinase were repressed.

RT-PCR analysis of the endogenous levels of G256 indicated that this gene sequence was expressed primarily in shoots, flowers, and siliques. A cDNA microarray experiment confirmed this tissue distribution data by RT-PCR. There was no induction of G256 in leaves or in seedlings in response to environmental stress treatments.

Potential Applications

The potential utility of this gene or its equivalogs is to confer better germination and growth in the cold. The germination of many crops is very sensitive to cold temperatures. A gene that would allow germination and seedling vigor in the cold would have tremendous utility in allowing seeds to be planted earlier in the season with a high rate of survivability.

G278 (SEQ ID NO ID NO: 47)

Published Information

G278 was identified by amino acid sequence similarity to plant and mammalian ankyrin-repeat proteins. G278 is on chromosome 1 BAC F15H21 (GenBank accession number AC066689.5; nid=12323462), released by the Arabidopsis Genome Initiative. The transcription start/stop codon was correctly predicted. G278 is referred to in the public literature as NPR1, a gene that controls the onset of systemic acquired resistance in plant (Cao et al. (1997) Cell 88:57-63; Cao et al. (1998) Proc. Natl. Acad. Sci. 95:6531-6536).

It was shown that a 2-3-fold overexpression of 35S::NPR1 over basal wild-type expression level results in transgenic plants resistant to the bacterial pathogen Psm ES4326 and the oomycete Peronospora parasitica Noco. An inducing signal (SA, INA, or a pathogen infection) is necessary for the expression of the phenotype and downstream induction of pathogen-related proteins.

Experimental Observations

RT-PCR analysis of the endogenous level of G278 transcripts revealed that G278 was present at moderate, constitutive level in all tissues examined. G278 expression levels were similar to the wild-type control in all the biotic/abiotic treatments examined. The function of G278 was analyzed in transgenic plants overexpressing G278 under the control of the 35S promoter. Transformants were morphologically indistinguishable from wild-type plants. Plants overexpressing G278 were more susceptible to infection with the necrotrophic fungal pathogen Sclerotinia sclerotiorum when compared with control plants. The experiment was confirmed on individual lines. Transgenic G278 overexpressing lines were similar to control wild-type plant when challenged with Botrytis cinerea and Fusarium oxysporum.

Data in the public literature indicated that G278/NPR1 plays an important role in mediating the onset of systemic acquired resistance in plant. Dual resistance of a 35S::NPR1 transgenic plants to bacterial and fungal pathogen suggested that G278/NPR1 may be key to the generation of broad-spectrum resistance in plant. The 35S::G278 overexpressor experimental data indicated that overexpressing G278 had little effect in improving resistance following infection with necrotrophic pathogens (Fusarium oxysporum, Botrytis cinerea, and Sclerotinia sclerotiorum). In fact, reduced tolerance of the transgenic 35S::NPR1 plant to infection with Sclerotinia sclerotiorum was observed. Although we cannot rule out the possibility of co-suppression in transgenic T2 lines tested, it is likely that that resistance to necrotrophic pathogens is mediated by different pathway than the SA/SAR pathway. Overexpression of G278 may directly or indirectly introduces competition for co-factor(s) or results in biochemical interference, which may be detrimental for proper development of resistance to Sclerotinia sclerotiorum.

Potential Applications

35S::G278 overexpression in Arabidopsis was shown to affect the onset of disease following inoculation with Sclerotinia sclerotiorum. Therefore, G278 or its equivalogs can be used to manipulate the defense response in plants.

G291 (SEQ ID NO: 49)

Published Information

G291 is referred to in the public literature as the Arabidopsis AJH1, a plant homolog of the c-Jun coactivator. AJH1 was isolated by peptide sequencing of a subunit of the COP9 complex, an important component in light-mediated signal transduction in Arabidopsis. It is postulated that the COP9 complex may modulate the activities of transcription factors in response to environmental stimuli. Localization experiment reveals that AJH1 was present in monomeric form, which suggested a possible involvement in other developmentally regulated processes (Kwok et al. (1998) Plant Cell 10:1779-1790). G291 is found in the sequence of the chromosome 1 BAC F19G10 (GenBank accession AF000657.1 GI:2098816), released by the Arabidopsis Genome Initiative. The start and stop codons were correctly predicted.

Experimental Observations

The expression profile of G291 revealed a low, but constitutive, expression of G291 transcripts in all tissues examined. G291 transcript levels were similar to the wild-type controls in all the physiological treatments examined as determined by RT-PCR analysis.

G291 overexpressors produced significantly more seed oil than wild-type plants.

Potential Applications

G291 or its equivalogs can be used to increase seed oil content, which may be of nutritional value for food for human consumption as well as animal feeds.

G303 (SEQ ID NO: 51)

Published Information

G303 corresponds to gene MNA5.5 (BAB11554.1).

Experimental Observations

The complete sequence of G303 was determined. G303 was detected at very low levels in roots and rosette leaves.

The function of this gene was analyzed using transgenic plants in which G303 was expressed under the control of the 35S promoter. G303 overexpressing plants had more tolerance to osmotic stress in a germination assay in three separate experiments. They had more seedling vigor than wild-type control when germinated on plates containing high salt and high sucrose. No altered morphological or biochemical phenotypes were detected in G303 overexpressing plants.

Potential Applications

G303 or its equivalogs may be useful for enhancing seed germination under high salt conditions or other conditions of osmotic stress. Evaporation from the soil surface causes upward water movement and salt accumulation in the upper soil layer where the seeds are placed. Thus, germination normally takes place at a salt concentration much higher than the mean salt concentration in the whole soil profile. Increased salt tolerance during the germination stage of a crop plant would impact survivability and yield. G303 can also be used to engineer plants with enhanced tolerance to drought, salt stress, and freezing.

G312 (SEQ ID NO: 53)

Published Information

G312 corresponds to a predicted SCWERECROW gene regulator in annotated P1 clone MUD21 (AB010700), from chromosome 5 of Arabidopsis (Kaneko et al. (1998) DNA Res. 5: 131-145).

Experimental Observations

The function of this gene was analyzed using transgenic plants in which G312 was expressed under the control of the 35S promoter. Transgenic plants overexpressing G312 were more salt tolerant than wild-type plants, as determined by a germination assay on MS media supplemented with 150 mM NaCl. G312 was constitutively expressed at very low levels in all tissues tested. Expression of G312 did not appear to be induced by any of the environmental or stress conditions tested.

Potential Applications

Transgenic plants overexpressing G312 germinated better in a high salt environment than control plants. These data suggested that G312 or its equivalogs can be used to create crop plants that are more tolerant of high salt conditions. Better germination in high salt conditions is desirable because, in the field, germination normally takes place at a salt concentration much higher than the mean salt concentration in the whole soil profile. This is because evaporation from the soil surface causes upward water movement and salt accumulation in the upper soil layer where the seeds are placed. Increased salt tolerance during the germination stage of a crop plant would impact survivability and yield.

G325 (SEQ ID NO ID NO: 55)

Published Information

G325 was identified as a gene in the sequence of chromosome 4, ESSA I FCA contig fragment No. 3 (GenBank Accession number Z97338), released by the European Union Arabidopsis Sequencing Project.

Experimental Observations

The function of G325 was analyzed using transgenic plants in which G325 was expressed under the control of the 35S promoter. G325 overexpressing plants had more tolerance to osmotic stress in a germination assay in three separate experiments. They had more seedling vigor than wild-type control when germinated on plates containing high salt and high sucrose. No altered morphological phenotypes or altered phenotypes in the biochemical assays were observed.

G325 was expressed at high levels in flowers and cauline leaves, and at lower levels in shoots, rosette leaves, and seedlings. G325 was induced by auxin, cold- and heat-stress. The expression of G325 also was reduced in response to Fusarium infection or salicylic acid treatment.

Potential Applications

G325 or its equivalogs may be useful for enhancing seed germination under high salt conditions or other conditions of osmotic stress. Evaporation from the soil surface causes upward water movement and salt accumulation in the upper soil layer where the seeds are placed. Thus, germination normally takes place at a salt concentration much higher than the mean salt concentration in the whole soil profile. Increased salt tolerance during the germination stage of a crop plant would impact survivability and yield.

G325 or its equivalogs can also be used to engineer plants with enhanced tolerance to drought, salt stress, and freezing, at later stages.

G343 (SEQ ID NO: 59)

Published Information

G343 was identified as GATA-2 (accession number Y13649) by homology to other GATA transcription factors.

Experimental Observations

RT-PCR and microarray data analysis of the endogenous levels of G343 indicated that this gene was ubiquitously expressed in all tissues albeit predominantly in seedling. In addition, G343 was repressed in response to treatment with Erysiphe and Fusarium.

The function of G343 was analyzed through its ectopic overexpression in plants. G343 overexpressors grew very poorly on soil, and T2 plants were not propagated for biochemical analysis. On the other hand, G343 overexpressors grew as well as wild-type controls on MS media, where they exhibited an increase in tolerance to glyphosate and oxidative stress. G343 T2 plants were rescued from the control plates, propagated to the next generation, and tested again on glyphosate plates where they exhibited the same resistance phenotype. Additional T2 lines were obtained and tested. A fourth line also showed a striking tolerance to glyphosate, though two other lines exhibited a slight increase in susceptibility to glyphosate. These opposite effects in the T2 lines might be caused by silencing of the gene. It would, therefore, be very interesting to determine the phenotype of G343 knockouts in glyphosate-resistance assays.

Potential Applications

G343 or its equivalogs can be used for the generation of glyphosate resistant plants, and to increase plant resistance to oxidative stress.

G353 (SEQ ID NO: 59)

Published Information

G353 was identified in the sequence of P1 clone MMN10, GenBank accession number AB0154751, released by the Arabidopsis Genome Initiative. G353 corresponds to RHL41 (Kazuoka et al. (2000) Plant J. 24:191-203) and Zat12 (Meissner et al. (1997) Plant Mol. Biol. 33:615-624). Transgenic Arabidopsis plants over-expressing the RHL41 gene showed an increased tolerance to high-intensity light, and also morphological changes of thicker and dark green leaves. The palisade parenchyma was highly developed in the leaves of the transgenic plants. Anthocyanin content, as well as the chlorophyll content, also increased. Antisense transgenic plants exhibited decreased tolerance to high irradiation. RHL41 protein may play a key role in the acclimatization response to changes in light intensity.

Experimental Observations

G353 was uniformly expressed in all tissues and under all conditions tested in RT-PCR experiments. The highest level of expression was observed in rosette leaves, embryos, and siliques. The function of this gene was analyzed using transgenic plants in which G353 was expressed under the control of the 35S promoter. Overexpression of G353 in resulted in enhanced tolerance to osmotic stress in one transgenic line. The most dramatic effect of overexpression of G353 was observed in flower morphology. 35S::G353 plants had a reduction in flower pedicel length, and downward pointing siliques. This phenotype was very similar to that described for the brevipedicellus (bp) mutant (Koornneef et al. (1983) J. Hered. 74:265-272) and in overexpression of a related gene, G354. Other morphological changes in shoots were also observed in 35S::G353 plants. Leaves had short petioles, were rather flat, rounded, and sometimes showed changes in coloration. These effects were observed in varying degrees in the majority of transformants. Severely affected plants were tiny, had contorted leaves, poor fertility, and produced few seeds. Overexpression of G353 in Arabidopsis resulted in an increase in seed glucosinolate M39494 in two T2 lines.

Potential Applications

G353 or its equivalogs can be used to alter inflorescence structure, which may have value in production of novel ornamental plants.

G353 or its equivalogs can be used to alter a plant's response to water deficit conditions and, therefore, be used to engineer plants with enhanced tolerance to drought, salt stress, and freezing.

Increases or decreases in specific glucosinolates or total glucosinolate content may be desirable depending upon the particular application. For example: (1) Glucosinolates are undesirable components of the oilseeds used in animal feed, since they produce toxic effects. Low-glucosinolate varieties of canola have been developed to combat this problem; (2) Some glucosinolates have anti-cancer activity; thus, increasing the levels or composition of these compounds might be of interest from a nutraceutical standpoint; (3) Glucosinolates form part of a plants natural defense against insects; modification of glucosinolate composition or quantity could therefore afford increased protection from predators; furthermore, in edible crops, tissue specific promoters might be used to ensure that these compounds accumulate specifically in tissues, such as the epidermis, which are not taken for consumption.

G354 (SEQ ID NO: 61)

Published Information

G354 was identified in the sequence of BAC clone F12M12, GenBank accession number AL355775, released by the Arabidopsis Genome Initiative. G354 corresponds to ZAT7 (Meissner et al. Plant Mol. Biol. 33:615-624).

Experimental Observations

Greatest levels of expression of G354 were observed in rosette leaves, embryos, and siliques. Some expression of G354 was also observed in flowers.

The function of this gene was analyzed using transgenic plants in which G353 was overexpressed under the control of the 35S promoter. 35S::G354 plants had a reduction in flower pedicel length, and downward pointing siliques. This phenotype was very similar to that described for the brevipedicellus (bp) mutant (Koornneef et al. (1983) J. Hered. 74:265-272) and in overexpression of a related gene, G353. Other morphological changes in shoots were also observed in 35S::G354 plants. Many 35S::G354 seedlings had abnormal cotyledons, elongated, thickened hypocotyls, and short roots. The majority of T1 plants had a very extreme phenotype, were tiny, and arrested development without forming inflorescences. T1 plants showing more moderate effects had poor seed yield.

Overexpression of G354 in Arabidopsis resulted in seedlings with an altered response to light. In darkness, G354 seedlings failed to etiolate. The phenotype was most severe in seedlings from one line where overexpression of the transgene resulted in reduced open and greenish cotyledons.

Potential Applications

G354 or its equivalogs can be used to alter inflorescence structure, which may have value in production of novel ornamental plants.

G354 modifies the light response and thus G354 or its equivalogs may be useful for modifying plant growth or development, for example, photomorphogenesis in poor light, or accelerating flowering time in response to various light intensities, quality or duration to which a non-transformed plant would not similarly respond. Elimination of shading responses may lead to increased planting densities with subsequent yield enhancement.

G361 (SEQ ID NO: 63)

Published Information

G361 was first isolated by Tague et al. ((1995) Plant Mol. Biol. 28:267-279) in an effort to study the sequence and the expression pattern of C2H2 zinc finger protein encoding genes in Arabidopsis (Takatsuji (1998) Cell. Mol. Life. Sci. 54:582-596). The latter study showed that G361 (ZFP6) was mostly expressed in roots and shoots based on Northern analysis.

Experimental Observations

A full-length cDNA was isolated and used to transform plants. G361 overexpressors were small and very late bolting. The plants did not show any physiological phenotype. G361 overexpressing plants had increased levels of polyunsaturated fatty acids. The phenotype could be related to the darker green color of the plants and their possible higher chlorophyll content (repeat of analysis also in progress). Higher 16:3 fatty acid content, in particular, could be a reflection of a higher chloroplast number or more chloroplast membranes. RT-PCR data showed that the gene was expressed mostly in shoots and in roots at low levels.

Potential Applications

The late-flowering phenotype of G361 or its equivalogs is useful in that late flowering is desirable in crops where the vegetative portion of the plant is harvested (often vegetative growth stops when plants make the transition to flowering). In this case, it can be advantageous to prevent or delay flowering in order to increase yield. Also, prevention of flowering can be useful in these same crops in order to prevent the spread of transgenic pollen and/or to prevent seed set. In any case, the overexpressors were clearly smaller, an undesirable phenotype which has to be corrected before overexpression of the gene can lead to any useful crop product.

G362 (SEQ ID NO: 65)

Published Information

G362 was identified in the sequence of BAC clone T10024, GenBank accession number AC007067, released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of this gene was analyzed using transgenic plants in which G362 was expressed under the control of the 35S promoter. 35S::G362 had a number of developmental effects with the most prominent result being an increase in trichome number as well as the ectopic formation of trichomes. Overexpression of G362 also increased anthocyanin levels in various tissues at different stages of growth. Seedlings sometimes showed high levels of pigment in the first true leaves. Late flowering lines also became darkly pigmented. Seeds from a number of lines were observed to develop patches of dark purple pigmentation. Inflorescences from 35S::G362 plants were thin, and flowers sometimes displayed poorly developed organs. The seed yield from many lines was somewhat poor. 35S::G362 transgenic plants showed no phenotypic alterations in response to the physiological or biochemical analyses performed.

As determined by RT-PCR, G362 was expressed in roots, and was expressed at significantly lower levels in siliques, seedlings, and shoots. No expression of G362 was detected in the other tissues tested. G362 expression was induced in rosette leaves by heat stress.

Potential Applications

G362 or its equivalogs can be used to alter anthocyanin production. The utilities of this gene includes alterations in pigment production for horticultural purposes, and possibly increasing stress resistance in combination with another transcription factor.

G362 or its equivalogs can be used to delay flowering in transgenic plants. This can have useful implications in crop plants. In species such as sugarbeet where the vegetative parts of the plants constitute the crop and the reproductive tissues were discarded, it would be advantageous to delay or prevent flowering. In addition, extending vegetative development could have a beneficial effect on yield, since the plants have a longer time to build up their photosynthetic capacity. This in turn can translate into larger accumulations of storage products.

G362 or its equivalogs can be used to alter trichome number and distribution in plants. Trichome glands on the surface of many higher plants produce and secrete exudates, which give protection from the elements and pests such as insects, microbes and herbivores. These exudates may physically immobilize insects and spores, may be insecticidal or anti-microbial or they may allergens or irritants to protect against herbivores. Trichome have also been suggested to decrease transpiration by decreasing leaf surface air flow, and by exuding chemicals that protect the leaf from the sun.

Another utility for G362 or its equivalogs is to increase the density of cotton fibers in cotton bolls. Cotton fibers are modified unicellular trichomes that are produced from the ovule epidermis. Typically only 30% of the epidermal cells take on a trichome fate (Basra et al. (1984) Int. Rev. Cytol. 89:65-113). Thus cotton yields might be increased by inducing a greater proportion of the ovule epidermal cells to become fibers.

Depending on the plant species, varying amounts of diverse secondary biochemicals (often lipophilic terpenes) are produced and exuded or volatilized by trichomes. These exotic secondary biochemicals, which are relatively easy to extract because they are on the surface of the leaf, have been widely used in such products as flavors and aromas, drugs, pesticides, and cosmetics. One class of secondary metabolites, the diterpenes, can effect several biological systems such as tumor progression, prostaglandin synthesis, and tissue inflammation. In addition, diterpenes can act as insect pheromones, termite allomones, and can exhibit neurotoxic, cytotoxic and antimitotic activities. As a result of this functional diversity, diterpenes have been the target of research several pharmaceutical ventures. In most cases where the metabolic pathways were impossible to engineer, increasing trichome density or size on leaves may be the only way to increase plant productivity.

Thus, the use of G362 and its homologs to increase trichome density, size, or type may therefore have profound utilities in so called molecular farming practices (for example, the use of trichomes as a manufacturing system for complex secondary metabolites), and in producing insect-resistant and herbivore-resistant plants.

G371 (SEQ ID NO: 67)

Published Information

G371 was identified as the published gene A-RZF accession number U81598, expressed preferentially in seed development (Zou et al. (1997) Gene 196:291-295).

Experimental Observations

The sequence of G371 was experimentally determined and the function of G371 was analyzed using transgenic plants in which G371 was expressed under the control of the 35S promoter. Plants overexpressing G371 appeared to be more sensitive to Botrytis infection. No altered morphological or biochemical phenotypes were observed for G371 overexpressing plants.

The function of this gene was also studied by knockout analysis. The phenotype of the G371 knockout was wild-type for all assays performed.

Array analysis of endogenous levels of G371 indicated that this gene was expressed predominantly in embryos, consistent with its published expression pattern. There was no change in the expression of this gene in response to environmental stress according to RT-PCR data. However, according to array data, this gene was induced 4-fold in response to Erysiphe infection, its expression was not affected by infection with Fusarium, and it was repressed 3-fold after a 12 hour treatment at 4° C.

Potential Applications

Because G371 confers sensitivity to Botrytis, this gene or its equivalogs has utility in producing pathogen resistant plants.

G390 (SEQ ID NO: 69)

Published Information

G390 was isolated by Ruzza et al. (GenBank Accession: CAD29544, gi:20069421) using degenerate oligonucleotides corresponding to a conserved 6 amino acid sequence from the helix-3 region of athb-1 and athb-2. It was named athb-9. The published Northern blot showed slightly higher level of expression in stems, and lower levels in leaves, flowers, roots, and siliques. The G390 protein shares very extensive amino acid identity with other HD-ZIP class III proteins that exist in Arabidopsis (for example, G391 and G438). HD-ZIP class III proteins are known to have complex roles in determining meristem development, vascular tissue formation, and stem lignification (Baima et al. (1995) Development 12:4171-4182; Baima et al. (2001) Plant Physiol. 126:643-655; Talbert et al. (1995) Development 121:2723-2735; Zhong et al. (1997) Plant Cell 9:2159-2170; Sessa et al. (1998) Plant Mol. Biol. 38:609-622; Zhong et al. (1999) Plant Cell 11:2139-2152; Ratcliffe et al. (2000) Plant Cell 12:315-317; and Otsuga et al. (2001) Plant J. 25:223-236).

Experimental Observations

Fourteen 35S::G390 T1 lines were obtained which displayed a consistent morphological phenotype; the majority of these plants were slightly small, had abnormal phyllotaxy, and exhibited stem bifurcations in which shoot meristems split to form two or three separate shoots. Additionally, a significant number of these extra T1 lines flowered earlier than controls. Comparable effects were obtained by overexpression of G391.

Potential Applications

The overexpression data suggest that G390 or its equivalogs has utility in the manipulation of shoot architecture. Additionally, since a number of the 35S::G390 lines flowered early, this gene or its equivalogs can be used to manipulate flowering time.

G391 (SEQ ID NO ID NO: 71)

Published Information

G391, also known as Athb-14, was isolated based on its homology with a previously identified homeobox containing gene, Athb-8 (G392). The full-length cDNAs encode proteins of 852 amino acids. Athb-8, -9 and -14 (G392, G390, and G391, respectively) are members of a small family of HD-Zip proteins (HD-ZIP III) characterized by a HD-Zip motif confined to the N-terminus of the polypeptide. The spatial organization of the HD-Zip domain of Athb-8, -9 and -14 is different from that of the Athb-1 (G409, a member of the HD-ZIP I family) and Athb-2 (G400, a member of the HD-ZIP II family) HD-Zip domains. DNA binding analysis performed with random-sequence DNA templates showed that the Athb-9 HD-Zip (HD-Zip-9) domain, but not the Athb-9 HD alone, binds to DNA. The HD-Zip-9 domain recognizes a 11 bp pseudopalindromic sequence (GTAAT(G/C)ATTAC) as determined by selecting high-affinity binding sites from random-sequence DNA. Moreover, gel retardation assays demonstrated that the HD-Zip-9 domain binds to DNA as a dimer. These data supported the notion that the HD-ZIP III domain interacts with DNA recognition elements in a fashion similar to the HD-ZIP I and II domains.

The G391 protein shares very extensive amino acid identity with other HD-ZIP class III proteins that exist in Arabidopsis (for example, G390 and G438). These genes are known to have complex roles in determining meristem development, vascular tissue formation, and stem lignification (Baima et al. (1995) Development 12:4171-4182; Baima et al. (2001) Plant Physiol. 126:643-655; Talbert et al. (1995) Development 121:2723-2735; Zhong et al. (1997) Plant Cell 9:2159-2170; Sessa et al. (1998) Plant Mol. Biol. 38:609-622; Zhong et al. (1999) Plant Cell 11:2139-2152; Ratcliffe et al. (2000) Plant Cell 12:315-317; and Otsuga et al. (2001) Plant J. 25:223-236).

Experimental Observations

The function of this gene was analyzed using transgenic plants in which G391 was expressed under the control of the 35S promoter. Although plants from the T2 generation were wild-type in morphology, the T1 plants showed significant deleterious effects. The plants were small and dark green with short bolts. All other phenotypes were wild-type in all assays performed. As determined by RT-PCR, G391 was moderately expressed in shoots, and was expressed at lower levels in roots, flowers, and rosettes.

An additional sixteen 35S::G391 T1 lines were obtained that displayed a consistent morphological phenotype; the majority of these plants were small, had abnormal phyllotaxy, and exhibited stem bifurcations in which shoot meristems split to form two or three separate shoots. Additionally a significant number of these extra T1 lines flowered earlier than controls. Comparable effects were obtained by overexpression of G390.

Potential Applications

The overexpression data suggested that G391 or its equivalogs have utility in the manipulation of shoot architecture. Additionally, since a number of the 35S::G391 lines flowered early, this gene or its equivalogs can be used to manipulate flowering time.

G409 (SEQ ID NO: 73)

Published Information

G409, also named Athb-1, was one of the earliest plant homeodomain leucine (HD-ZIP) zipper genes cloned. It was isolated from a cDNA library by highly degenerate oligonucleotides corresponding to a conserved eight amino acid sequence from the helix-3 region of the homeodomain. The protein was found to transactivate a promoter linked to a specific DNA binding site (CAATTATTG) by transient expression assays. Overexpression of Athb-1 affected the development of palisade parenchyma under normal growth conditions, resulting in light green sectors in leaves and cotyledons, whereas other organs in the transgenic plants remained normal.

Experimental Observations

G409 was induced by drought and repressed by NaCl. Plants overexpressing G409 were more tolerant to infection by the fungal pathogen Erysiphe orontii. In addition to the Erysiphe tolerant phenotype, the overexpressors were slightly early flowering.

Potential Applications

The expression of transcription factors such as G409 or its equivalogs involved in plant/pathogen interaction can be modulated to manipulate the plant defense- wound- or insect-response in order to generate pathogen resistant plants.

G427 (SEQ ID NO: 75)

Published Information

G427 corresponds to KNAT4, one of four KNOX class II homeobox genes in Arabidopsis. This gene was originally identified by Serikawa et al. ((1996) Plant Mol. Biol. 32:673-683) using low-stringency screening of Arabidopsis cDNA libraries using the knl homeobox from maize. No genetic characterization of KNAT4 have been published, but it is expressed at high levels in leaves and young siliques (Serikawa et al. (1996) supra). It should be noted that KNAT4 shares a very high level of sequence similarity with another KNOX class II gene, KNAT3 (G426). Expression of each of these genes is light dependent, suggesting that that they might have a role in light regulated developmental processes (Serikawa et al. (1996) supra; Serikawa et al. (1997) Plant J. 11:853-861).

Experimental Observations

The function of G427 was assessed by analysis of transgenic Arabidopsis lines in which the cDNA was constitutively expressed under the control of the 35S CaMV promoter.

35S::G427 transformants flowered markedly earlier than wild-type controls in conditions of either continuous light or a 12-hour photoperiod. Such results indicated that G427 can promote flowering in Arabidopsis under either inductive or non-inductive conditions. These data correlated well with the published observation that G427 expression is light regulated, and indicated that the gene likely has a function in the regulation of flowering time in Arabidopsis. Additionally, 35S::G427 seedlings were noted to have rather vertically positioned leaves, a feature that is often apparent in plants with abnormal light regulated development.

Overexpression of G427 in Arabidopsis also resulted in an increase in seed oil and a decrease in seed protein in two T2 lines. No other phenotypic alterations were observed.

Potential Applications

G427 or its equivalogs can be used to manipulate seed oil and seed protein content, which may be of nutritional value for human consumption, and for animal feeds.

G427 or its equivalogs can be used to regulate flowering time in commercial species. A wide range of potential applications exist; prevention of flowering might help maximize vegetative yields and prevent escape of GMO pollen, whereas accelerating flowering could shorten crop and tree breeding programs.

Additionally, G427 or its equivalogs can be used in inducible systems that could be used to synchronize flowering in a crop.

G438 (SEQ ID NO: 77)

Published Information

G438 was identified as a homeobox gene (MUP 24.4) within P1 clone MUP 24 (GenBank accession number AB005246). G438 was identified as the Arabidopsis REVOLUTA (REV) gene (Ratcliffe et al. (2000) Plant Cell 12:315-317). Based on its mutant phenotype, REV had previously been identified as having a key role in regulating the relative growth of apical versus non-apical (cambial) meristems (Alvarez (1994) in Arabidopsis: An Atlas of Morphology and Development (ed. J. Bowman), pp. 188-189, New York, N.Y.: Springer-Verlag; Talbert et al. (1995) Development 121:2723-2735). The revoluta phenotype was highly pleiotropic but was characterized by a failure in development of all types of apical meristem: lateral shoot meristems in the axils of cauline and rosette leaves were often completely absent, or replaced by a solitary leaf. These effects were most evident in higher order shoots, but in some cases, the primary shoot meristem also failed and terminated growth in a cluster of filamentous structures. Rev floral meristems often failed to complete normal development and form incomplete or abortive filamentous structures. In contrast to apical meristems, structures formed by non-apical meristems, such as leaves, stems, and floral organs often became abnormally large and contorted in the rev mutant.

The features of rev mutants were similar to those of the interfascicular fiberless1 (ifl1) mutant. Ifl1 was isolated during screens for mutants lacking normal stem fiber differentiation (Zhong et al. (1997) Plant Cell 9:2159-2170). Wild-type Arabidopsis plants form interfascicular fibers which became lignified and added support to the inflorescence stem (Aloni (1987) Annu. Rev. Plant Physiol. 38:179-204); Zhong et al. (1997) supra; Zhong et al. (1999) Plant Cell 11:2139-2152). In the ifl1 mutant, normal interfascicular fibers were absent and the differentiation of both xylary fibers and vessel elements was disrupted. In addition to these internal features, ifl1 mutants had secondary morphological features very similar to those of rev. Recently the IFL1 gene was cloned by Zhong et al. (1999 supra). It was found that the IFL1 sequence and map position were identical to those of the REV gene cloned, demonstrating that REV and IFL1 are the same gene. (Ratcliffe et al. (2000) supra).

It had been suggested that REV promotes the growth of apical meristems (including floral meristems) at the expense of non-apical meristems (Talbert et al. (1995) supra). It is not yet clear, however, whether expression data support such a role: strong expression of REV has been detected in interfascicular regions and developing vascular tissue, but in-situ expression analysis of apical meristems has not yet been reported. (Zhong et al. (1999) supra). REV is a group III HD-ZIP protein and shares high sequence similarity (and organization) with the proteins encoded by three other Arabidopsis genes: Athb8, Athb9, and Athb 14 (Sessa et al. (1998) Plant Mol. Biol. 38:609-622). It is possible, therefore, that these genes act together in the same developmental process. Supporting this suggestion, Athb8 had a similar expression pattern to REV and was transcribed in the procambial regions of vascular bundles (Baima et al. (1995) Development 12:4171-4182).

Experimental Observations (Knockout)

G438 was initially identified as MUP24.4, a novel putative homeobox gene within P1 clone MUP24 (GenBank Accession AB005246). Annotation was confirmed by isolation of the G438 cDNA: the cDNA had an in-frame stop codon immediately 5′ to the predicted start codon and comprised 18 exons that had been predicted within the genomic sequence.

Plants homozygous for a T-DNA insertion in the G438 sequence were obtained by PCR based screening of DNA pools from the Jack Collection of insertional mutants (Campisi et al. (1999) Plant Journal 17:699-707). The T-DNA insertion was located 466 bp downstream of the putative start codon, and was predicted to create a null mutation. The mutation was recessive and produced a revoluta phenotype. Complementation crosses and sequencing of a known revoluta allele demonstrated that G438 was REVOLUTA.

RT-PCR analyses detected G438 expression at medium to high levels in all tissues and conditions tested. Further expression analysis was possible since the T-DNA insertion contained an enhancer trap construct (Campisi et al. (1999) supra). GUS staining could therefore be used to reveal the expression pattern of genes within which insertions occurred. GUS staining of seedlings homozygous and heterozygous for the G438 T-DNA insertion revealed very strong expression within axillary shoots. This expression data correlates with the marked effects of the rev mutation on outgrowth of higher order shoots.

Experimental Observations (Overexpressor)

A full-length clone was amplified from cDNA derived from mixed tissue samples, and 35S::G438 transformants were generated. These lines appeared wild-type in the physiological assays, but showed differences in morphology compared with control plants. At early stages, a small number of T1 plants displayed aberrant phyllotaxy and were rather dwarfed, but these effects were inconsistent, and the majority of lines appeared wild-type. At later stages, however, around half of the primary transformants, from two of the three T1 sowings, developed slightly larger flatter leaves than wild type at late stages. The progeny of four lines that had shown these phenotypes were examined in the T2 generation. At late stages, plants from two of these T2 populations again displayed slightly broad flat leaves, but plants from the other two T2 populations appeared wild-type at all stages.

A single T1 plant line out of a total of 37 lines had highly aberrant shoot meristem development. At the early seedling stage, it appeared as though the primary shoot apex of this individual had developed into a terminal leaf-like structure. Subsequent growth then continued from an axillary shoot meristem that initiated from the base of a cotyledon petiole. However, this effect became silenced between generations and was not observed in the T2 progeny from one line. Given that this effect was observed in only a single line, it could have been the result of an activation tagged locus at the T-DNA insertion site, rather than due to G438 expression. However, the phenotype would fit with a role for REV in regulating apical meristem development.

Potential Applications

The mutant phenotypes indicated that REV/IFL1 or its equivalogs have an important role in determining overall plant architecture and the distribution of lignified fiber cells within the stem. A number of utilities can be envisaged based upon these functions.

Modifying the activity of REVOLUTA orthologs from tree species can offer the potential for modulating lignin content. This can allow the quality of wood used for furniture or construction to be improved. Lignin is energy rich; increasing lignin composition could therefore be valuable in raising the energy content of wood used for fuel. Conversely, the pulp and paper industries seek wood with a reduced lignin content. Currently, lignin must be removed in a costly process that involves the use of many polluting chemicals. Consequently, lignin is a serious barrier to efficient pulp and paper production (Tzira et al. (1998) TIBTECH 16:439-446; Robinson (1999) Nature Biotechnology 17:27-30). In addition to forest biotechnology applications, changing lignin content might increase the palatability of various fruits and vegetables.

In Arabidopsis, reduced REV activity results in a reduction of higher-order shoot development. Reducing activity of REV orthologs may generate trees that lack side branches, and have fewer knots in the wood. Altering branching patterns can also have applications amongst ornamental and agricultural crops. For example, applications might exist in any species where secondary shoots currently have to be removed manually, or where changes in branching pattern could increase yield or facilitate more efficient harvesting.

G450 (SEQ ID NO: 79)

Published Information

G450 is IAA14, a member of the Aux/IAA class of small, short-lived nuclear proteins that contain four conserved domains. IAA14 was found as one of a group of Arabidopsis IAA genes that was isolated based on homology to early auxin-induced genes of pea (Abel et al. (1995) J. Mol. Biol. 251:533-549). Recently a gain-of-function mutant in IAA14, slr (solitary root), was found to abolish lateral root formation, reduce root hair formation, and impair gravitropic responses (Fukaki (2001) Abstracts 12th Intl. Conf. Arabidopsis Res. #448, Madison, Wis.).

Experimental Observations

Overexpression of G450 influenced leaf development, overall plant stature, and seed size. 35S::G450 plants produced seeds that were larger than wild-type seed.

Potential Applications

G450 or its equivalogs can used to produce larger seed in plants, which may positively influence seed storage characteristics, appearance and yield.

G464 (SEQ ID NO: 81)

Published Information

G464 is IAA12, a member of the Aux/IAA class of small, short-lived nuclear proteins that contain four conserved domains. IAA12 was found as one of a group of Arabidopsis IAA genes that was isolated based on homology to early auxin-induced genes of pea. IAA12 transcripts were modestly (2 to 4-fold) induced by auxin, with optimal induction at 10 μM auxin (Abel et al. (1995) J. Mol. Biol. 251:533-549).

Experimental Observations

G464 overexpressing Arabidopsis lines showed enhanced germination in high heat conditions. In addition, one Arabidopsis line overexpressing G464 showed an increase in total seed protein and a decrease in total seed oil by NIR in one assay.

Potential Applications

G464 or its equivalogs in native or altered form is useful to produce plants that germinate better in hot conditions.

G470 (SEQ ID NO: 83)

Published Information

A partial cDNA clone corresponding to G470 was isolated in a two-hybrid screen for proteins that interact with ARF1, a transcription factor that binds to auxin response elements, and this clone was named ARF1 Binding Protein (Ulmasov et al. (1997) Science 276:1865-1868). A full-length clone was later isolated, and the gene was renamed ARF2 (Ulmasov et al. (1999a) Proc. Natl. Acad. Sci. 96:5844-5849). ARF2 was shown to bind to an auxin response element (Ulmasov et al. (1999b) Plant J. 19:309-319).

Co-transfection of ARF2 and a reporter construct with an auxin response element into carrot protoplasts did not result in either activation or repression of transcription of the reporter gene (Ulmasov et al. (1999a) supra). ARF2 binding to palindromic auxin response elements is thought to be facilitated by dimerization mediated by the carboxy-terminal domain of ARF2 (Ulmasov et al. (1999b) supra). It is possible that ARF2 regulates gene expression through heterodimerization with other ARF proteins or with IAA proteins. ARF2 was found to be expressed uniformly in roots, rosette leaves, cauline leaves, flowers, and siliques (Ulmasov et al. (1999b) supra).

Experimental Observations

Expression of a truncated G470 clone in the antisense orientation under the 35S promoter caused infertility in Arabidopsis. In primary transformants expressing the G470 clone, the stamens failed to elongate properly. Pollen was produced, but was not deposited on the stigma. The transformants appeared otherwise morphologically normal. Because of the infertility of the primary transformants, no material was available for biochemical and physiological analyses. The truncated clone corresponds to the carboxy-terminal portion of the ARF2 protein, and lacks the DNA binding domain.

Potential Applications

G470 or its equivalogs are useful in engineering infertility in self-pollinating plants.

G477 (SEQ ID NO ID NO: 85)

Published Information

G477 corresponds to SPL6 (AJ011643, Cardon et al. (1999) Gene 237:91-104), a member of the SBP family of transcription factors. G477 is expressed constitutively throughout the development of Arabidopsis. Outside the SBP-domain, G477 has a putative myc-like helix-loop-helix dimerization domain (Cardon et al. (1999) supra).

Experimental Observations

The complete sequence of G477 was determined. The function of this gene was analyzed using transgenic plants in which G477 was expressed under the control of the 35S promoter. The phenotype of these transgenic plants was wild-type in all morphological and biochemical assays performed.

Plants overexpressing G477 were slightly more sensitive to the herbicides glyphosate and acifluorfen and to oxidative stress caused by rose bengal compared with wild-type controls. Plants overexpressing G477 also develop more disease symptoms following inoculation with a moderate dose of Sclerotinia sclerotiorum compared with control plants. It is well known that oxidative stress is a component of a plant defense response to pathogen and therefore the disease susceptibility phenotype could be related to a general sensitivity to oxidative stress.

G477 was expressed in all tissues and under all conditions tested in RT-PCR and cDNA microarray experiments.

Potential Applications

G477 activity was shown to affect the response of transgenic plants to the fungal pathogen Sclerotinia sclerotiorum and oxidative stress tolerance. Therefore, G477 or its equivalogs can be used to manipulate the defense response in order to generate pathogen-resistant plants.

G481 (SEQ ID NO: 87)

Published Information

G481 is equivalent to AtHAP3a which was identified by Edwards et al. ((1998) Plant Physiol. 117:1015-1022) as an EST with extensive sequence homology to the yeast HAP3. Northern blot data from five different tissue samples indicated that G481 was primarily expressed in flower and/or silique, and root tissue.

Experimental Observations

G481 was analyzed through its ectopic overexpression in plants. G481 overexpressors were more tolerant to high sucrose in a germination assay. The phenotype of G481 was mild; however, there was a consistent difference in the hypocotyl and root elongation in the overexpressor plants compared to wild-type controls. Sucrose-sensing has been implicated in the regulation of source-sink relationships in plants. Consistent with the sugar sensing phenotype of the G481 overexpressors were the results from the biochemical analysis of G481 overexpressor plants indicating that one line had higher amounts of seed oils and lower amounts of seed protein. This suggested that G481 was involved in the allocation of storage compounds to the seed. One G481 overexpressor line was darker green in the T2 generation, which could mean a higher photosynthetic rate consistent with the possible role of G481 in sugar sensing.

G481 overexpressing plants were found to be more tolerant to drought in a soil-based assay.

Potential Applications

The utility of G481 or its equivalogs includes a role in sugar sensing, a plant mechanism that has been shown to be involved in the following: 1) altering storage compound accumulation (oil and/or protein) in seeds which could impact yield and seed quality, and 2) altering photosynthetic rate which could also impact yield in vegetative tissues as well as seed. G481 was shown to alter sugar sensing. Sugars are key regulatory molecules that affect diverse processes in higher plants including germination, growth, flowering, senescence, sugar metabolism and photosynthesis. Sucrose is the major transport form of photosynthate and its flux through cells has been shown to affect gene expression and alter storage compound accumulation in seeds (source-sink relationships).

The enhanced germination phenotype of transgenic plants overexpressing G481 under a condition of drought or osmotic stress (such as high concentrations of sucrose) suggested the gene or its equivalogs can also be used to improve plant tolerance to water deficit related conditions such as water deprivation, salt stress, and freezing stress. Thus, G481 can be used to engineer plants with enhanced stress tolerance that can ultimately impact survivability and yield.

G482 (SEQ ID NO: 89)

Published Information

G482 is equivalent to AtHAP3b which was identified by Edwards et al. ((1998) Plant Physiol. 117:1015-1022) as an EST with homology to the yeast gene HAP3b. Edwards' northern blot data suggests that AtHAP3b is expressed primarily in roots. No other functional information regarding G482 is publicly available.

Experimental Observations

G482 function was analyzed through its ectopic overexpression in plants under the control of a 35S promoter. G482 overexpressors were more tolerant to high NaCl in a germination assay.

RT-PCR analysis of endogenous levels of G482 transcripts indicated that this gene was expressed constitutively in all tissues tested. A cDNA array experiment supported the RT-PCR derived tissue distribution data. G482 was not induced above basal levels in response to any environmental stress treatments tested.

Potential Applications

The utilities of this gene or its equivalogs include the ability to confer salt tolerance during the germination stage of a crop plant. This would most likely impact survivability and yield. Evaporation of water from the soil surface causes upward water movement and salt accumulation in the upper soil layer, where the seeds were placed. Thus, germination normally takes place at a salt concentration much higher than the mean salt concentration in the whole soil profile.

G484 (SEQ ID NO: 91)

Published Information

G484 is equivalent to ATHDR1B and was isolated by Kuromori et al. ((1994) Nucleic Acids Res. 22:5296-5301). The Arabidopsis sequence is highly homologous to the human DR1 gene that has been shown to interact with TATA-binding protein (TBP) to repress transcription of class II genes (Yeung et al. (1994) Genes Dev. 8:2097-2109).

Experimental Observations

Homozygous knockout mutant plants as well as plants ectopically overexpressing G484 were used to determine the function of this gene in Arabidopsis. Insertion of T-DNA into G484 at nucleotide position +439 with respect to the start ATG codon was within the first third of the G484 coding sequence of the gene and therefore was likely to result in a null mutation. The phenotype for G484 overexpressor and knockout mutant plants was similar to wild-type for all morphological, biochemical and physiological assays performed. RT-PCR analysis of the endogenous levels of G484 transcripts indicated that this gene was expressed primarily in shoots, roots and flowers, with a low level expression in the other tissues tested. G484 was not induced significantly above basal levels in response to any environment stress treatments tested.

Potential Applications

G484 knockout mutant seed had an altered glucosinolate profile and therefore the gene or its equivalogs can be used to modify glucosinolate composition in plants.

G489 (SEQ ID NO: 93)

Published Information

G489 was identified from a BAC sequence that showed high sequence homology to AtHAP5-like transcription factors in Arabidopsis. No published information is available regarding the function of this gene.

Experimental Observations

The function of G489 was analyzed through its ectopic overexpression in plants. G489 overexpressors were more tolerant to high NaCl stress, showing more root growth and leaf expansion compared with the controls in culture. Two well characterized ways in which NaCl toxicity is manifested in the plant is through general osmotic stress and potassium deficiency due to the inhibition of its transport. These G489 overexpressor lines were more tolerant to osmotic stress in general, showing more root growth on mannitol containing media.

RT-PCR analysis of endogenous levels of G489 transcripts indicated that this gene was expressed constitutively in all tissues tested. A cDNA array experiment confirmed the RT-PCR derived tissue distribution data. G489 was not induced above basal levels in response to the stress treatments tested.

Potential Applications

The utilities of this gene or its equivalogs include the ability to confer salt tolerance during the growth and developmental stages of a crop plant. This would impact yield and or biomass.

G490 (SEQ ID NO: 95)

Published Information

G490 is member of the Hap5-like subfamily of the CAAT-box binding transcription factors. G490 was identified in the sequence of BAC MXA21, GenBank accession number AB005247, released by the Arabidopsis Genome Initiative.

Experimental Observations

The complete sequence of G490 was determined. The function of this gene was analyzed using transgenic plants in which G490 was expressed under the control of the 35S promoter. The phenotype of these transgenic plants was wild-type in all physiological assays performed. Overexpression of G490 resulted in a marked early flowering phenotype under continuous light conditions.

During initial studies on lines #1-20, plants were not carefully examined for flowering time, and at later developmental stages, appeared to have a wild-type phenotype. To assess flowering time more carefully, a further batch of 35S::G490 T1 plants were grown. The majority of these plants showed a very clear acceleration of flowering and had visible flower buds up to a week earlier than wild type. At later stages the plants appeared wild-type. To confirm these observations, T2 progeny from three early flowering T1 plants were grown; all three T2 lines showed early flowering.

In addition to the flowering time phenotype, seed of 35S::G490 transgenic plants showed altered tocopherol composition. In seeds of two lines, an increase in the percentage of delta-tocopherol was observed.

As determined by RT-PCR, G490 was expressed at low levels in flower, rosette leaf, embryo and silique. No expression of G490 was detected in the other tissues tested. G490 expression is induced to low levels in rosette leaves by auxin treatment, drought, heat, osmotic and salt stress treatments.

Potential Applications

One utility of a gene such as G490 or its equivalogs is to accelerate flowering.

In addition, G490 or its equivalogs can be used to alter tocopherol composition. Tocopherols have anti-oxidant and vitamin E activity.

G504 (SEQ ID NO: 97)

Published Information

G504 was identified in the sequence of BAC F11P17, GenBank accession number AC002294, released by the Arabidopsis Genome Initiative.

Experimental Observations

The complete sequence of G504 was determined. The function of this gene was analyzed using transgenic plants in which G504 was expressed under the control of the 35S promoter. The phenotype of these transgenic plants was wild-type in all physiological and biochemical assays performed. 35S::G504 transgenic plants had a subtle leaf phenotype in the early developmental stages but were wild-type in appearance in later stages of development. In one transgenic line, a decrease in seed oil as measured by NIR was observed. Also, seeds of this same line also showed an increase in the percentage of 18:2 fatty acid and a decrease in the percentage of 20:1 fatty acid.

In an RT PCR experiment, endogenous G504 appeared to be expressed specifically and at high levels in flower tissue. No induction of endogenous G504 expression in leaf tissue was detected in response to any environmental conditions tested.

Potential Applications

G504 or its equivalogs may be used to modify seed oil content in seeds, which may be very important for the nutritional value and production of various food products. The promoter of G504 can be used to engineer flower specific gene expression.

G509 (SEQ ID NO: 99)

Published Information

G509 was identified in the sequence of BAC F20O9, GenBank accession number AL021749, released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of G509 was analyzed using transgenic plants in which G509 was expressed under the control of the 35S promoter, as well as using a line homozygous for a T-DNA insertion in G509. The T-DNA insertion of G509 at nucleotide position +1583 with respect to the start ATG codon was approximately half way into the coding sequence of the gene and therefore was likely to result in a null mutation. G509 primary transformants showed no significant morphological differences from control plants, though one T2 line was noted to be small and sickly at the seedling and rosette stages, and pale and late flowering at the flowering stage. Knockout plants showed no consistent morphological differences from controls. G509 knockout plants may be more susceptible to infection with a moderate dose of the fungal pathogen Erysiphe orontii; 8 out of 8 plants tested showed more fungal growth compared with the wild-type controls. G509 lines had significantly higher levels of chlorophyll a, and lower levels of chlorophyll b in seeds.

G509 knockout mutants produced more seed oil and more seed protein than wild-type control plants.

Endogenous G509 was expressed constitutively in all tissues tested, with the highest levels of expression in shoots, roots, flowers and siliques.

Potential Applications

G509 or its equivalogs can be used to produce plants with altered seed oil and seed protein content.

G509 or its equivalogs can be used to manipulate the defense response in order to generate pathogen-resistant plants.

In addition, G509 or its equivalogs can be used to regulate the levels of chlorophyll in seeds.

G519 (SEQ ID NO: 101)

Published Information

G519 was first identified in the sequence of the P1 clone MBK5, GenBank accession number AB005234, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

A related gene to G519 is the rice gene OsNAC6 (GenBank accession number BAA89800).

Experimental Observations

The function of G519 was analyzed with transgenic plants in which G519 was expressed under the control of the 35S promoter.

RT-PCR analysis was used to determine the endogenous levels of G519 in a variety of tissues and under a variety of environmental stress-related conditions. G519 was constitutively expressed with the highest level of expression in shoots, roots and seedlings. RT-PCR data also indicated an induction of G519 transcripts accumulation upon auxin, abscisic acid (ABA), cold, heat, Fusarium and salicylic acid (SA) treatments.

As measured by NIR, G519 overexpressors were found to have increased seed oil content compared to wild-type plants.

Potential Applications

G519 or its equivalogs may be used to alter seed oil content in plants, which may be very important for the nutritional value and production of various food products.

G545 (SEQ ID NO: 103)

Published Information

G545 was discovered independently by two groups. Lippuner et al. (1996) J. Biol. Chem. 271:12859-12866) identified G545 as an Arabidopsis cDNA (STZ), which increases the tolerance of yeast to Li+ and Na+. They found that STZ expression is most abundant in leaves and roots, and that its level of expression increases slightly upon exposure of the plant to salt. The second group (Meissner et al (1997) Plant Mol. Biol. 33:615-624), identified G545 (ZAT10) in a group of Arabidopsis C2H2 zinc finger protein-encoding cDNAs that they isolated by degenerate PCR. According to their data, ZAT10 is expressed in roots, shoots and stems.

Closely Related Genes from Other Species

A closely related non-Arabidopsis sequence is a cDNA from the nitrogen-fixing species Datisca glomerata (AF119050). The similarity of this sequence with G545 extends beyond the conserved domain.

Experimental Observations

Plants overexpressing G545 flowered early, and in extreme cases were infertile. G545 overexpression conferred tolerance of transgenic plants to phosphate deficiency. This could be the result of insensitivity to phosphate, higher rates of phosphate assimilation or larger stores of phosphate. G545 overexpressors also appeared to be more sensitive to NaCl than wild-type plants. This result was unexpected, since yeast cells overexpressing G545 are more tolerant to salt stress than control cells. There may be a dominant negative effect in plants, triggered by the over-accumulation of the G545 protein, which does not exist in yeast.

G545 overexpressing plants appeared to be significantly more susceptible to pathogens than control plants. This implied a role for the G545 in the control of defense mechanisms.

Potential Applications

G545 or equivalog overexpression may result in tolerance to phosphate deficiency. Young plants have a rapid intake of phosphorous, so it is important that seed beds have high enough content in phosphate to sustain their growth. Also, root crops such as carrot, potato and parsnip will all decrease in yield if there is insufficient phosphate available. Phosphate costs represent a relatively small but significant portion of farmers' operating costs (3-4% of total costs to a corn farmer in the US, higher to a vegetable grower). Plants that are tolerant to phosphate deficiency can represent a cost saving for farmers, especially in areas where soils are very poor in phosphate.

Another desirable phenotype, salt tolerance, may arise from G545 or equivalog silencing rather than overexpression. Additionally, G545 appeared to be induced by cold, drought, salt and osmotic stresses, which was in agreement with a potential role of the genes in protecting the plant in such adverse environmental conditions.

G545 also appears to be involved in the control of defense processes. However, overexpression of G545 made Arabidopsis plants more susceptible to disease. This negative effect will have to be corrected before G545 can be used in a crop to induce tolerance to low phosphate, such as by restricting overexpression of G545 or its equivalogs to roots.

G546 (SEQ ID NO: 105)

Published Information

G546 was identified in the sequence of P1 clone MJB20 and BAC clone T19E12, GenBank accession number AC007584, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G546 homologs in other species are Y14573.1:33104.33991 from barley, OSJNBb0064P21.7 from rice.

Experimental Observations

RT-PCR was used to analyze the endogenous levels of G546 transcripts. RT-PCR data indicated that G546 was expressed constitutively in all tissues examined. There was a moderate level of G546 transcript detected in shoots and roots while in flowers, rosette and cauline leaves, and siliques transcript level was low. G546 transcripts were not elevated in response to the environmental stress treatments.

The function of this gene was analyzed using transgenic plants in which G546 was expressed under the control of the 35S promoter. Overexpression of G546 in Arabidopsis resulted in one line in which seedlings were ABA insensitive in a germination assay. Morphologically, the plants were small at early stages, grew slowly, became dark colored, and senesced late. Somewhat similar effects were observed in approximately half of the primary transformants. 35S::G546 transformants also sporadically displayed increased anthocyanin levels in cotyledons at the seedling stage, young leaves, and in the stems of secondary shoots.

Potential Applications

G546 appears to affect ABA sensitivity, therefore, G546 or its equivalogs may have a utility in modifying ABA responses such as seed dormancy and drought tolerance.

In addition, G546 or its equivalogs could be used to alter anthocyanin production. The potential utilities of this gene include alterations in pigment production for horticultural purposes, and increasing stress resistance, possibly in combination with another transcription factor.

G561 (SEQ ID NO: 107)

Published Information

G561 is the Arabidopsis gene GBF2 (Schindler et al (1992) EMBO J. 11:1261-1273), which was cloned by hybridization to GBF1. GBF2 is constitutive in both light and dark grown leaves, expressed in roots, and the nuclear import of GBF1 may be light regulated (Terzaghi et al (1997) Plant J. 11:967-982).

Closely Related Genes from Other Species

Close relatives of G561 include a G-box binding protein from Sinapis alba (Y16953; unpublished) and a G-Box binding protein from Raphanus sativus (X92102, unpublished).

Experimental Observations

The function of G561 was analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter. Plants over-expressing G561 showed more root growth on potassium free media. Expression of G561 also appears to be constitutive, and may be preferentially expressed in siliques and moderately inducible with heat stress.

An important aspect of the potassium root growth assay is that plants were firstly germinated on media with potassium and then transferred onto potassium-free media. G561 overexpressors may have be able to somehow cope with less potassium, and it is also possible that G561 overexpressors accumulated more potassium before they were transferred, which allowed the roots to grow more vigorously after transfer.

As measured by NIR, G561 overexpressors were found to have increased seed oil content compared to wild-type plants.

Potential Applications

G561 or its equivalogs could be used to increase seedling vigor or plant growth in soils that are low in potassium. Potassium is a macronutrient required for a variety of basic plant functions which is commonly added to soil as a fertilizer. The ability to grow plants on low potassium soils may save the ecological and material cost of soil fertilization.

G561 or its equivalogs may also be used to manipulate sterol composition, and may be used to modify seed oil content in plants, which may be very important for the nutritional value and production of various food products.

G562 (SEQ ID NO: 109)

Published Information

G562 is the published Arabidopsis transcription factor GBF3, which was cloned through its hybridization with GBF1 (Schindler et al. (1992) EMBO J. 11: 1261-1273). GBF3, like GBF1 and GBF2, can bind G-box elements as a homodimer, or as a heterodimer with other bZIP family members. GBF3 appears to be highly expressed in roots in comparison to leaves, and repressed by light. GBF3 binds to G-box elements in the Arabidopsis ADH promoter in vitro, is induced by ABA in suspension cultures, and is proposed to be the transcription factor responsible for the ABA regulated ADH gene expression (Lu et al. (1996) Plant Cell. 8:847-857).

Closely Related Genes from Other Species

Similar genes to G562 include the B. napus proteins BnGBF1 and BnGBF2 (U27107 and U27108) which are strikingly similar to G562 for their entire lengths. An unpublished Catharanthus roseus G-box binding protein 1 protein (AF084971) also has significant homology to G562 outside of the conserved domain.

Experimental Observations

G562 appeared to be preferentially expressed in root and flower tissues by RT-PCR analysis, and expressed at lower levels in other tissues of the plant. G562 was induced by heat, drought and osmotic stress in seedlings. The function of G562 was analyzed using transgenic plants in which G562 was expressed under the control of the 35S promoter. Plants overexpressing G562 were consistently and significantly later flowering, with more crinkled leaves than wild-type plants.

Potential Applications

G562 or its equivalogs could be used to manipulate flowering time in plants.

G567 (SEQ ID NO: 111)

Published Information

G567 was discovered as a bZIP gene in BAC T10P11, accession number AC002330, released by the Arabidopsis genome initiative.

Closely Related Genes from Other Species

G567 is similar to two bZIP factors from Petroselinum crispum (1806261) and Glycine max (1905785). Similarity between these two proteins and the protein encoded by G567 extends beyond the conserved domains and thus they may have a function and utility to G567.

Experimental Observations

The annotation of G567 in BAC AC002330 was experimentally confirmed and the function of G567 was analyzed using transgenic plants in which G567 was expressed under the control of the 35S promoter.

Seedlings overexpressing G567 had slowly opening cotyledons and very short roots when grown on MS plates containing glucose. G567 is thus likely to be involved in sugar sensing or metabolism during germination.

As measured by NIR analysis, plants overexpressing G567 had an increase in total combined seed oil and seed protein content.

G567 appears to be constitutively expressed, and induced in leaves in a variety of conditions.

Potential Applications

G567 or its equivalogs may be useful in manipulating seed oil and protein content.

G567 or its equivalogs may be used to modify sugar sensing.

In addition to their important role as an energy source and structural component of the plant cell, sugars are central regulatory molecules that control several aspects of plant physiology, metabolism and development. It is thought that this control is achieved by regulating gene expression and, in higher plants, sugars have been shown to repress or activate plant genes involved in many essential processes such as photosynthesis, glyoxylate metabolism, respiration, starch and sucrose synthesis and degradation, pathogen response, wounding response, cell cycle regulation, pigmentation, flowering and senescence.

Because sugars are important signaling molecules, the ability to control either the concentration of a signaling sugar or how the plant perceives or responds to a signaling sugar could be used to control plant development, physiology or metabolism. For example, the flux of sucrose (a disaccharide sugar used for systemically transporting carbon and energy in most plants) has been shown to affect gene expression and alter storage compound accumulation in seeds. Manipulation of the sucrose signaling pathway in seeds may therefore cause seeds to have more protein, oil or carbohydrate, depending on the type of manipulation. Similarly, in tubers, sucrose is converted to starch which is used as an energy store. It is thought that sugar signaling pathways may partially determine the levels of starch synthesized in the tubers. The manipulation of sugar signaling in tubers could lead to tubers with a higher starch content.

Thus, manipulating the sugar signal transduction pathway may lead to altered gene expression to produce plants with desirable traits. In particular, manipulation of sugar signal transduction pathways could be used to alter source-sink relationships in seeds, tubers, roots and other storage organs leading to increase in yield.

G568 (SEQ ID NO: 113)

Published Information

G568 was identified in the sequence of BAC T19K4, GenBank accession number AL022373, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

The PTBF1 gene from Populus×generosa appears to be a potential homolog of G568 (GenBank accession no AF288616). PTBF1 expression is associated with terminal bud formation.

Experimental Observations

The annotation of G568 in BAC AL022373 was experimentally confirmed. G568 appeared to be preferentially expressed in shoots, roots and flowers and its expression was strongly repressed by the fungal pathogens Erysiphe orontii and Fusarium oxysporum, indicating that in some way it could be a repressor of a defense response.

The function of this gene was analyzed using transgenic plants in which G568 was expressed under the control of the 35S promoter. Plants overexpressing G568 displayed a variety of morphological phenotypes. These morphological phenotypes include narrow leaves, a darker green coloration, and bushy, spindly, poorly fertile shoots, dwarfing and flowering time alteration. No disease-related phenotype was observed.

Potential Applications

G568 or its equivalogs may be used to manipulate plant architecture and flowering time. The expression pattern of G568 also indicated a use for this gene or its equivalogs in manipulating the defense response. The promoter of G568 may also have some utility as a promoter that can be used to engineer down-regulation of gene expression in response to pathogen attack.

G584 (SEQ ID NO: 115)

Published Information

G584 was identified in chromosome IV BAC T6K21 sequence (gene T6K21.10) by the EU Arabidopsis sequencing project as “bHLH protein-like”.

Closely Related Genes from Other Species

A related gene to G584 is Phaseolus vulgaris phaseolin G-box binding protein PG1 (U18348). Similarity between G584 and PG1 extends beyond the signature motif of the family. No functional information is available for gene PG1 other than that the protein binds to a G-box motif CACGTG of the bean seed storage protein beta-phaseolin gene.

Experimental Observations

The function of G584 was analyzed using transgenic plants in which G584 was expressed under the control of the 35S promoter. G584 transgenic plants seemed to produce seed of a larger size than control plants. Analysis of G584 overexpressors revealed no apparent physiological or biochemical changes when compared to wild-type control plants. Analysis of the endogenous expression level of G584, as determined by RT-PCR, revealed a moderate and constitutive expression level in all Arabidopsis tissues examined. G584 transcript level remained similar to wild-type controls in all the treatments examined.

Potential Applications

G584 or its equivalogs could be used to produce larger seed size and/or altered seed morphology, which may positively influence seed storage characteristics, appearance and yield.

G585 (SEQ ID NO: 117)

Published Information

G585 has been identified as GL3. It has been shown that G585 regulates trichome development in Arabidopsis through interaction with GL1 and TTG1 (Payne et al. (2000) Genetics. 156:1349-1362). An increase in the trichome density was observed in GL3 overexpressed transgenic plants in WS background.

Closely Related Genes from Other Species

G585 protein shares a significant homology to GHDEL65 [Gossypium hirsutum] protein (PID:g13346182) as well as DEL [Antirrhinum majus] protein (PID:g166428).

Experimental Observations

The sequence of G585 was experimentally determined and the function of G585 was analyzed using transgenic plants in which G585 was expressed under the control of the 35S promoter.

Overexpression of G585 reduced trichome density on leaves and stems. Since this phenotype was confined to a proportion of plants in a single T2 line, it could have been due to co-suppression. To examine this, a second selection of T1 plants was screened: one out of 18 of these plants exhibited a clear reduction in trichome density. Since the glabrous effects were eventually seen in two independent lines, they most likely represented a low penetrance G585 overexpression phenotype or co-suppression. An increase in the trichome density was not observed in G585 overexpressed transgenic plants in an ecotype background. These results are different from the published information, and may simply be due to the difference in ecotype used.

As determined by RT-PCR, G585 was uniformly expressed at low level in all tissues tested. Expression level of G585 appears to be enhanced by auxin treatments and repressed by pathogen Fusarium infections.

Potential Applications

G585 or its equivalogs can be used to affect trichome number and/or distribution. A transcription factor that alters trichome number could be used to increase the production of chemical compounds (like essential oils) that are synthesized and/or stored in trichomes, as well as to protect plants against damage from a variety of herbivores.

G590 (SEQ ID NO ID NO: 119)

Published Information

The sequence of G590 was obtained from the Arabidopsis genome sequencing project, GenBank accession number Z99707, based on its sequence similarity within the conserved domain to other bHLH/Myc related proteins. A knockout mutant in G590, named as SPATULA, has also been isolated and characterized (Heisler et al. (2000) Development 128:1089-1098).

Experimental Observations

The function of this gene was studied by knockout analysis and by using transgenic plants in which G590 was expressed under the control of the 35S promoter.

G590 knockout plants produced more seed oil than wild-type controls.

Overexpression of G590 resulted in a reduction in flowering time and a shorter generation time. Under continuous light conditions, G590 overexpressing plants typically produced visible flower buds approximately one week earlier than wild-type controls. At the time of bolting, these plants had 4-8 rosette leaves compared with 8-11 in wild type. Additionally, G590 overexpressor had rather pointed leaves at early stages of development. The plants also appeared slightly small, yellow, and later, had elongated leaf petioles. No other physiological and biochemical alterations were observed in the overexpression transgenic plants when compared to wild-type controls.

Gene expression profiling using RT-PCR shows that G590 was relatively expressed at higher levels in flowers, siliques and roots. Its expression level was unaffected by any of the conditions tested.

Potential Applications

G590 or its equivalogs could be used to increase seed oil content, which would be of nutritional value for food for human consumption as well as animal feeds.

Based on the current analysis of G590 overexpressing plants, G590 or its equivalogs could be used to manipulate flowering time. A wide variety of applications exist for systems that shorten the time to flowering.

G594 (SEQ ID NO: 121)

Experimental Observations

The function of this gene was studied using transgenic plants in which G594 was expressed under the control of the 35S promoter.

Plants overexpressing G594 showed more disease symptoms following infection with the necrotrophic fungal pathogen Sclerotinia sclerotiorum compared to control plants. In a repeat experiment on individual lines, two lines showed the enhanced susceptibility phenotype. No other consistent morphological or biochemical differences were observed between G594 overexpressors and wild-type plants.

RT-PCR analysis of G594 transcripts indicate that G594 was constitutively expressed in all tissues with exception of roots. The expression level of G594 was induced by auxin treatments and repressed by cold, Erysiphe and Fusarium treatments.

Potential Applications

Since G594 transgenic plants have an altered response to the necrotrophic fungal pathogen Sclerotinia sclerotiorum, G594 or its equivalogs could be used to manipulate the defense response in order to generate pathogen-resistant plants.

G597 (SEQ ID NO: 123)

Published Information

G597 was identified in the sequence of BAC F4P9, GenBank accession number AC002332, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G597 has significant homology to a DNA-binding protein PD1 [Pisum sativum] and an Oryza sativa putative AT-Hook DNA-binding protein (PID:12643044).

Experimental Observations

The function of this gene was studied using transgenic plants in which G597 was expressed under the control of the 35S promoter.

Approximately half of the G597 primary transformants were observed to have narrow curled rosette leaves. Four T1 plants were also observed to be later bolting than wild type. However, these phenotypes were not apparent in the initial plantings or re-plants of the T2 populations.

Overexpression of G597 in one line caused an alteration in the leaf cell wall polysaccharide composition. An increase in the percentage of xylose and a decrease in the percentage of rhamnose was detected. Otherwise, G597 overexpressors behaved similarly to wild-type controls in all biochemical assays performed.

Based on the RT-PCR analysis, G597 was constitutively expressed in all tissues. Lower expression levels were observed in siliques and cauline leaves. Its expression level was unaffected by any of the conditions tested.

As measured by NIR, G597 overexpressors were found to have increased seed oil and decreased seed protein content as compared to wild-type plants.

Potential Applications

G597 or its equivalogs may be used to alter seed protein content in plants, which may be very important for the nutritional value and production of various food products.

G598 (SEQ ID NO: 125)

Published Information

G598 was identified in chromosome II BAC T6D20 sequence (gene T6D20.23) by The Institute for Genomic Research as an “unknown protein”.

Experimental Observations

cDNAs representing two splice variants of G598 were identified. These splice variants differ in the 3′ end region and would produce proteins with different C-termini. The function of G598 was analyzed using transgenic plants in which splice variant number 1 of G598 was expressed under the control of the 35S promoter. G598 overexpressors had higher seed oil content in all three lines tested when measured by NIR. These three lines also showed increased galactose levels when insoluble sugar composition was determined. Otherwise, G598 overexpressors behaved similarly to wild-type controls in all biochemical assays performed. The characterization of G598 overexpressors revealed no apparent morphological or physiological changes when compared to wild-type control plants. Analysis of the endogenous expression level of G598, as determined by RT-PCR, revealed a moderate and constitutive expression level in all tissues and conditions examined.

One transgenic line showed a reproducible increase in galactose in leaves.

Potential Applications

On the basis of the biochemical analyses performed to date, G598 or its equivalogs may play a role in the accumulation or regulation of leaf insoluble sugars. Insoluble sugars are among the building blocks of plant cell walls. Transcription factors that alter plant cell wall composition such as galactose have several potential applications including altering food digestibility, plant tensile strength, wood quality, pathogen resistance and in pulp production. In particular, increasing the insoluble carbohydrate content in various fruits, vegetables, and other edible consumer products will result in enhanced fiber content. Increased fiber content would not only provide health benefits in food products, but might also increase digestibility of forage crops.

G598 or its equivalogs could be used to increase seed oil content, which would be of nutritional value for food for human consumption as well as animal feeds.

G634 (SEQ ID NO ID NO: 127)

Published Information

G634 was initially identified as public partial cDNAs sequences for GTL1 and GTL2 which are splice variants of the same gene (Smalle et al (1998) Proc. Natl. Acad. Sci. USA 95:3318-3322). The published expression pattern of GTL1 shows that G634 is highly expressed in siliques and not expressed in leaves, stems, flowers or roots.

Closely Related Genes from Other Species

A close non-Arabidopsis relative of G634 is O. sativa the gt-2 gene (2) which is proposed to bind and regulate the phyA promoter. In addition, the pea DNA-binding protein DF1 (13786451) shows strong homology to G634. The homology of these proteins to G634 extends to outside of the conserved domains and thus these genes are likely to be orthologs of G634.

Experimental Observations

The boundaries of G634 were experimentally determined and the function of G634 was investigated by constitutively expressing G634 using the CaMV 35S promoter.

Three constructs were made for G634: P324, P1374 and P1717. P324 was found to encode a truncated protein. P1374 and P1717 represent full length splice variants of G634; P1374, the shorter of the two splice variants was used for the experiments described here and the coding sequence of the P1374 clone is provided as the cDNA sequence for G634 in the Sequence Listing. The longest available cDNA (P1717), confirmed by RACE, had the same ATG and stop codons as the genomic sequence. Only data for P1374 are presented here.

Plants overexpressing G634 from construct P1374 had a dramatic increase the density of trichomes, which were also larger in size. The increase in trichome density was most noticeable on later arising rosette leaves, cauline leaves, inflorescence stems and sepals with the stem trichomes being more highly branched than controls. Approximately half of the primary transformants and two of three T2 lines showed the phenotype. Apart from slight smallness, there did not appear to be any other clear phenotype associated with the overexpression of G634. However, a reduction in germination was observed in T2 seeds grown in culture.

RT PCR data showed that G634 was preferentially expressed in flowers and germinating seedlings, and induced by auxin.

Potential Applications

G634 or its equivalogs may be used to alter trichome structure, function or density. Trichome glands on the surface of many higher plants produce and secrete exudates that give protection from the elements and pests such as insects, microbes and herbivores. These exudates may physically immobilize insects and spores, may be insecticidal or ant-microbial or they may allergens or irritants to protect against herbivores. Trichomes have also been suggested to decrease transpiration by decreasing leaf surface air flow, and by exuding chemicals that protect the leaf from the sun.

Depending on the plant species, varying amounts of diverse secondary biochemicals (often lipophilic terpenes) are produced and exuded or volatilized by trichomes. These exotic secondary biochemicals, which are relatively easy to extract because they are on the surface of the leaf, have been widely used in such products as flavors and aromas, drugs, pesticides and cosmetics. One class of secondary metabolites, the diterpenes, can effect several biological systems such as tumor progression, prostaglandin synthesis and tissue inflammation. In addition, diterpenes can act as insect pheromones, termite allomones, and can exhibit neurotoxic, cytotoxic and antimitotic activities. As a result of this functional diversity, diterpenes have been the target of research several pharmaceutical ventures. In most cases where the metabolic pathways are impossible to engineer, increasing trichome density or size on leaves may be the only way to increase plant productivity.

Thus, the use of G634 and its homologs to increase trichome density, size or type may therefore have profound utilities in so called molecular farming practices (i.e. the use of trichomes as a manufacturing system for complex secondary metabolites), and in producing resistant insect and herbivore resistant plants.

G635 (SEQ ID NO: 129)

Published Information

G635 was first identified in the sequence of BAC-end B67864, released by the Arabidopsis Genome Initiative. Subsequently, the full sequence of G635 was identified in BAC AB007649, also released by the Arabidopsis Genome Initiative.

Experimental Observations

The boundaries of G635 were experimentally determined and the function of G635 was analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter. Several plants over-expressing G635 were non-clonally sectored for chloroplast development and/or chlorosis. This phenotype seemed to correlate inversely with the expression level of the transgene, and plants over-expressing the highest amounts of G635 were wild-type in appearance. G635 over-expressing plants were otherwise wild-type biochemically and physiologically. G635 was constitutively expressed.

In the T2 generation, the bleaching phenotype did not show until plants started to flower, and the bleaching seemed to spread throughout the plant into areas that were previously green. This observation, in combination with the fact that the phenotype seems to be correlated with low expression of the gene, indicated that the phenotype was induced by silencing of G635.

A number of plants transformed with G635 had a variegated appearance.

Potential Applications

Based on the phenotype produced when G635 or its equivalogs may have a utility as a regulator of chloroplast development. In addition, G635 may be a herbicide target—if its activity or expression could be reduced using a small molecule, it could potentially kill the plant by causing chlorosis. G635 could also be developed into a marker for silencing in Arabidopsis.

The variegated phenotype associated with G635 or equivalog overexpression may find utility in ornamental applications.

G636 (SEQ ID NO: 131)

Published Information

G636 was identified through partial EST AA395524, released by Michigan State University. The entire sequence of G636 was later identified in BAC F7012, accession number F7012, released by the Arabidopsis genome initiative.

Closely Related Genes from Other Species

G636 is closely related to the Pisum sativum DNA-binding protein DF1, accession number AB052729, which may bind to light regulatory elements.

Experimental Observations

The 5′ boundary of G636 was determined and the function of G636 was analyzed by constitutively expressing the gene using the CaMV 35S promoter. Overexpression of G636 resulted in premature senescence of leaves and reduced plant size and fertility. No other phenotypic alterations were noted as a result of physiological or biochemical analyses.

G636 was constitutively expressed.

Potential Applications

G636 or its equivalogs may be used to alter senescence responses in plants. Although leaf senescence is thought to be an evolutionary adaptation to recycle nutrients, the ability to control senescence in an agricultural setting has significant value. For example, a delay in leaf senescence in some maize hybrids is associated with a significant increase in yields and a delay of a few days in the senescence of soybean plants can have a large impact on yield. Delayed flower senescence may also generate plants that retain their blossoms longer and this may be of potential interest to the ornamental horticulture industry.

G638 (SEQ ID NO: 133)

Published Information

G638 was identified in the sequence of BAC F17C15, GenBank accession number AL162506, released by the Arabidopsis Genome Initiative. During the course of its functional analysis, G638 was identified as the PETAL LOSS gene (Griffith et al. (1999) Development: 126:5635-5644). The PETAL LOSS knockout mutant displays a variety of flower phenotypes, most strikingly characterized by a reduction in the number of petals. In addition to flower organ number, organ identity, shape and orientation, particularly of petals, is altered.

Closely Related Genes from Other Species

A relative of G638 is a Medicago truncatula gene represented by the EST BF646615, which was isolated from an elicited cell culture cDNA library.

Experimental Observations

The boundaries of G638 were experimentally determined and the function of G638 was analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter. Expression of G638 causes severe alterations of plant development. The most striking feature of these overexpressor plants was that they have multipetallate flowers. In early flowers, some homeotic conversion had occurred between some organs of the flower. In all flowers made after these early flowers, petal number had been altered. Up to eight petals were consistently observed on plants that flowered, and as the plants grew older, the number of petals on new flowers was reduced from eight to about five. This phenotype was somewhat opposite to the phenotype observed with PETAL LOSS knockout plants and confirms a role for G638 in counting or maintaining petal number within the Arabidopsis flower. In addition to the flower phenotype, G638 caused alterations in phyllotaxy, leaf shape and caused plants to be sterile. G638 appears to be constitutively expressed.

Potential Applications

G638 or its equivalogs could be used to manipulate plant architecture and leaf shape, in particular this gene could be used to increase or decrease petal number in flowers. Overexpression of G638 also causes sterility, indicating there may be some use for this gene in engineering sterility into commercially relevant species.

G652 (SEQ ID NO: 135)

Published Information

G652 was identified in the sequence of BAC clones F26H11 and F7O24, GenBank accession number AC006264, released by the Arabidopsis Genome Initiative.

Experimental Observations (Knockout)

G652 appears to be constitutively expressed at medium levels in all tissues and environmental conditions tested as determined by RT-PCR analysis. Expression of G652 was not detected in other tissues. A line homozygous for a T-DNA insertion in G652 was used to determine the function of this gene. The T-DNA insertion of G652 was approximately 75% into the coding sequence of the gene and therefore was likely to result in a null mutation. Plants homozygous for a T-DNA insertions within G652 displayed a spectrum of developmental abnormalities, particularly at the early seedling stage. These phenotypes were variable within the population, suggesting that other factors might be influencing the penetrance of the phenotype. For example, seedlings were small and filled with anthocyanins. Almost all the seedlings had defects in cotyledons ranging from unusual shape to fusions. Many seedlings did not survive, and those that did grew slowly. Fertility was reduced compared to controls, senescence delayed, and siliques were often rather short. The reason for this poor fertility was unclear. Many flowers had a reduced number of stamens (4-5 of these organs rather than 6). Interestingly, the absent stamen(s) were usually one or both of the shorter pair. Seeds produced by knockouts of G652 plants were somewhat wrinkled and misshapen.

The G652 knockout line had a reproducible increase in the leaf glucosinolate M39480. It also showed a reproducible increase in seed alpha-tocopherol. A decrease in seed oil as measured by NIR was also observed.

Experimental Observations (Overexpressor)

The function of G652 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G652 resulted in plants that were small and slow developing. Many plants died at an early stage of growth. The two lines that were morphologically examined in the T2 generation were small and showed premature senescence of rosette leaves.

35S::G652 plants were wild-type in physiological analyses that were performed.

Potential Applications

G652 or its equivalogs could be used to manipulate seed tocopherol composition and seed structure and to alter glucosinolate composition in leaves.

G652 may also be useful for modifying glucosinolate content. Increases or decreases in specific glucosinolates or total glucosinolate content might be desirable depending upon the particular application.

G663 (SEQ ID NO: 137)

Published Information

G663 was identified from the Arabidopsis EST sequence, H76020, based on its sequence similarity within the conserved domain to other Myb family members in Arabidopsis. This gene was named MYB90 (Kranz et al. (1998) Plant J. 16:263-276). Reverse Northern data suggested G663 is expressed highly in leaves, siliques, and flowers and is induced by ethylene treatment.

Experimental Observations

The function of G663 was analyzed by its ectopic overexpression in plants. G663 overexpressors had constitutive anthocyanin production in seeds and roots. One line had higher anthocyanin production in leaf tissue as well. In other overexpressing lines, constitutive anthocyanin production was noted in trichomes and leaf margins. The overproduction of pigment in select tissues suggests there may be another transcription factor with which G663 interacts to activate the pathway. Using the corn system as a model, the interacting protein may be a bZIP like transcription factor.

RT-PCR analysis of the endogenous levels of G663 indicated that this gene was expressed primarily in siliques and seedlings. Array data confirmed the high levels in silique and also detected high levels of G663 in germinating seed tissue. G663 transcripts were also induced above basal levels by all stress treatments tested except by infection with Erysiphe orontii. These data were consistent with G663 being involved in the anthocyanin biosynthetic pathway, which is part of a common multi-stress response pathway.

Potential Applications

The potential utilities of this gene or its equivalogs includes alterations in pigment production for horticultural purposes, and possibly increasing stress resistance in combination with another transcription factor. Flavonoids have antimicrobial activity and could be used to engineer pathogen resistance. Several flavonoid compounds have health promoting effects such as the inhibition of tumor growth and cancer, prevention of bone loss and the prevention of the oxidation of lipids. Increasing levels of condensed tannins, whose biosynthetic pathway is shared with anthocyanin biosynthesis, in forage legumes is an important agronomic trait because they prevent pasture bloat by collapsing protein foams within the rumen. For a review on the utilities of flavonoids and their derivatives, refer to Dixon et al. ((1999) Trends Plant Sci. 10: 394-400).

G664 (SEQ ID NO: 139)

Published Information

G664 was identified from the Arabidopsis EST sequence, N38154, based on its sequence similarity within the conserved domain to other Myb family members in Arabidopsis. The Myb consortium named this gene MYB4 (Kranz et al. (1998) Plant J. 16: 263-276). Reverse Northern data suggested G664 is expressed highly in silique tissue with a low level of expression detected in all other tissues.

Closely Related Genes from Other Species

G664 shows extensive homology to the tomato gene THM27 (X95296) and the barley gene (X70877).

Experimental Observations

The function of G664 was analyzed through its ectopic overexpression in plants. G664 overexpressors germinated better and then developed more rapidly in cold conditions (8° C.) than wild-type controls. No differences in germination rates were observed on control MS media or in response to any other stress. Array data indicated that G664 was normally expressed primarily in root, shoot and silique.

Potential Applications

The potential utility of this gene or its equivalogs is to confer improved cold germination and/or growth. The germination of many crops like cotton is very sensitive to cold temperatures, a gene that would allow germination and seedling vigor in the cold would have tremendous utility in allowing seeds to be planted earlier in the season with a high rate of survivability.

G674 (SEQ ID NO: 141)

Published Information

G674 is a member of the (R1)R2R3 subfamily of myb transcription factors. G674 was identified in the sequence of BAC clone T2J13 with accession number AL132967 released by the Arabidopsis genome initiative. G674 has also been referred to as MYB45 (Kranz H D, et al. (1998) Plant J. 16:263-276). No information is available about the function(s) of G674.

Experimental Observations

The function of G674 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G674 transformants were generally rather smaller than wild-type controls, and possessed rounded, dark green leaves that were sometimes pointed upward. Overexpression of G674 also resulted in an increase in seed glucosinolate M39501 in two T2 lines. No other phenotypes were associated with the overexpression of G674.

RT-PCR analysis of endogenous levels of G674 indicated that this gene was expressed in all tissues except shoot. Expression levels of G674 seemed to vary in response to stress-related treatments.

Potential Applications

On the basis of the analyses performed to date, G674 or its equivalogs could be used to alter plant growth and development. In addition, overexpression of G674 caused changes in the seed glucosinolate profile.

G676 (SEQ ID NO: 143)

Published Information

G676 was identified from an Arabidopsis EST, N96391, based on its sequence similarity to other members of the Myb family within the conserved domain. The Myb consortium named this gene MYB66 (Kranz H D, et al. (1998) Plant J. 16:263-276) and in a report by Lee et al ((1999) Cell 1999 24; 99:473-483) a detailed functional analysis of G676, or “werewolf”, is described. Werewolf (WER) is involved in position-dependent patterning of epidermal cell types. Transcripts were localized to root epidermal cells that will develop into non-hair cells. WER was shown to regulate the position-dependent expression of GLABRA2, to interact with the maize R gene, and to act as an antagonist to the myb protein CAPRICE (G225). These authors do not report altered trichome positioning in their 35S:wer overexpressors.

Experimental Observations

The function of G676 was analyzed through its ectopic overexpression in plants. Morphologically, the plants are small, and partially glabrous on the upper surface of the leaf. Ectopic trichomes developed on the underside of the leaf in one line. Lee et al (1999) Cell 99: 473-483) fail to report altered trichome phenotypes in the leaves of the 35S:were overexpression lines. The present lines showed a higher degree of overexpression, which could explain the small stature of the plants as well.

RT-PCR analysis of the endogenous levels of G676 indicated that this gene was expressed primarily in roots with a low level of expression in siliques and seedlings. G676 transcripts were not induced significantly above basal levels by any stress-related treatments tested. In disease-related treatments where whole seedlings were harvested, transcripts were detectable but not above basal levels. This may be related to the gene's root expression. G676 transcripts were not found in Fusarium oxysporum treated seedlings; it is possible this treatment represses G676 expression in the roots.

Potential Applications

The potential utility of G676 or its equivalogs is the production of ectopic trichomes on the surface of the leaf. It would be of significant agronomic value to have plants that exhibit greater numbers of glandular trichomes producing essential oils for the pharmaceutical and food industries, as well as oils that protect plants against insect and pathogen attack.

G680 (SEQ ID NO ID NO: 145)

Published Information

G680 or LHY (late elongated hypocotyl) is an unusual Myb transcription factor in that it contains a single Myb repeat instead of the two repeat sequences found in the majority of plant Myb genes (R2R3Mybs). There are over 30 members of this single repeat Myb-related subfamily in the Arabidopsis genome. Both signature repeats in R2R3Myb domain are required for sequence specific DNA binding. However, the Myb-related subfamily with a single repeat domain are also able to bind to DNA in a sequence-specific manner (Baranowskij et al. (1994) EMBO J. 13: 5383-5392; Feldbrugge et al. (1997) Plant J. 11: 1079-1093) and are therefore thought to function as transcription factors.

G680 or LHY overexpression affects many processes associated with the circadian clock including, the rythmicity in both leaf movement, and the expression of CAB and CCR2 genes, as well as photoperiodic control of flowering time (Schaffer et al. (1998) Cell 93: 1219-1229). Other reported pleiotropic effects include elongated hypocotyls, elongated petioles, and pale leaves (Schaffer et al. (1998) Cell 93: 1219-1229). All of these phenotypes could potentially be explained by the impairment of circadian clock function. LHY shows a high degree of homology to CCA1, another protein implicated in circadian clock function (Wang et al. (1997) Plant Cell 9: 491-507).

Experimental Observations

The function of G680 was analyzed through its ectopic overexpression in plants. G680 overexpressors were late flowering under both short and long day conditions, however, the late flowering phenotype appeared more consistently under short day conditions. The overexpressors were darker green in color compared to the wild-type controls at later stages of development. This was inconsistent with the published phenotype, which indicates the plants have less chlorophyll, and are pale in color (Schaffer et al. (1998) Cell 93: 1219-1229). Preliminary data indicated that a vernalization treatment applied to germinating seedlings partially overcame the delay in flowering in the G680 overexpressors. Vernalized plants showed an approximate 35% reduction in leaf number on average compared to non-vernalized controls. Overexpression of G680 in plants also resulted in sensitivity to media containing high glucose in a germination assay, indicating a potential role for G680 in sugar sensing.

As determined by RT-PCR, G680 was uniformly expressed in all tissues tested. RT-PCR data also indicated a moderate induction of G680 transcripts accumulation upon drought treatment, and Erysiphe treatment could repress the expression of this gene.

Potential Applications

G680 or its equivalogs may be used to alter sugar sensing in plants. Sugars are key regulatory molecules that affect diverse processes in higher plants including germination, growth, flowering, senescence, sugar metabolism and photosynthesis. Sucrose is the major transport form of photosynthate and its flux through cells has been shown to affect gene expression and alter storage compound accumulation in seeds (source-sink relationships). Glucose-specific hexose-sensing has been described in plants and implicated in cell division and repression of ‘famine’ genes (photosynthetic or glyoxylate cycles). The potential utilities of a gene involved in glucose-specific sugar sensing are to alter energy balance, photosynthetic rate, carbohydrate accumulation, biomass production, source-sink relationships, and senescence.

Potential utilities of G680 or its equivalogs also include the regulation of flowering time. An area in which late flowering might be useful include crops where the vegetative portion of the plant is the marketable portion. In this case, it would be advantageous to prevent or delay flowering in order to increase yield. Prevention of flowering would also be useful in these same crops in order to prevent the spread of transgenic pollen and/or to prevent seed set.

A vernalization treatment applied to germinating G680 seedlings will partially overcome the delay in flowering in the G680 overexpressors. Vernalized plants showed an approximate 35% reduction in leaf number on average compared to non-vernalized controls. Various late flowering mutants are partially rescued by GA applications (Chandler et al. (1994) J. Exp. Bot. 45: 1279 1288). Thus it is possible that G680 could be used to increase the vegetative phases of development in order to increase yield and then triggered to flower via a cold treatment or a gibberelic acid application.

G682 (SEQ ID NO: 147)

Published Information

G682 was identified from the Arabidopsis BAC, AF007269, based on sequence similarity to other members of the Myb family within the conserved domain.

Experimental Observations

The function of G682 was analyzed through its ectopic overexpression in plants. G682 overexpressors were glabrous, had tufts of more root hairs and germinated better under heat stress conditions. Older plants were not more tolerant to heat stress compared to wild-type controls.

RT-PCR analysis of the endogenous levels of G682 transcripts indicated that this gene was expressed in all tissues tested, however, a very low level of transcript was detected in roots and shoots. Array tissue print data indicated that G682 was expressed primarily, but not exclusively, in flower tissue.

An array experiment was performed on one G682 overexpressing line. The data from this one experiment indicated that this gene could be a negative regulator of chloroplast development and/or light dependent development because the gene Albino3 and many chloroplast genes are repressed. Albino3 functions to regulate chloroplast development (Sundberg et al (1997) Plant Cell 9:717-730). The gene G682 was itself induced 20-fold. Other than a few additional transcription factors, very few genes are induced as a result of the ectopic expression of G682.

A number of plants transformed with G682 lacked trichomes.

Plants overexpressing paralogs of G682, including G225, G226 and G1816, have similar traits as plants that overexpress G682. These traits include reduction or lack of trichomes and increased root hairs, the latter indicating improved resistance to osmotic stress Plants overexpressing G676 and G1332 also have reduced trichome density. G676 and G1332 share 52% (21 of 40 residues) and 60% (24 of 40 residues) identity with G682, respectively, and 62% (20 of 32 residues) and 68% (22 of 32 residues) with the conserved domain of G682, respectively.

The polypeptide sequence of G682 shares 70% (50 of 71 residues), 66% (37 of 56 residues), and 57% (43 of 75 residues) identity with the conserved domains of G225, G226 and G1816, respectively. The conserved domain of G682 shares 86% (32 of 37 residues), 63% (23 of 36 residues), and 69% (25 of 36 residues) identity with the conserved domains of G225, G226 and G1816, respectively.

In addition to the paralogous sequences disclosed above, orthologous sequences from other plant species were also identified using BLAST analysis. Such orthologous sequences, together with the paralogous sequences were determined to be members of the G682 TF family of Myb-related proteins (equivalogs). The paralogous sequences and the orthologous sequences were aligned using MACVECTOR software (Accelrys, Inc.). The software program also generated an exemplary consensus amino acid residue sequence of the aligned sequences.

As shown in FIGS. 3A and 3B, the orthologous sequences shared a consensus sequence with the conserved domain of G682 (amino acid residues 27-63 of SEQ ID NO:148) and also shared identity with regions flanking the conserved domain (flanking regions). In particular, G682 shared a region of the conserved domain with sequences from soy (Glycine max; SEQ ID NOs: 1084, 1085, 1086, 1083, 1087, and 1088), rice (Oryza sativa; SEQ ID NOs: 559, 1082, and 1081), and maize (corn) (Zea mays; SEQ ID NOs: 1089 and 1090).

An exemplary consensus of the conserved domain of the G682 TF family of Myb-related proteins is Val-Xaa-Met/Phe-Ser/Thr-Gln/Glu-Xaa-Glu-Glu-Asp-Leu-Val-Xaa-Arg-Met-His/Tyr-Lys/Arg-Leu-Val-Gly-Asp/Glu-Arg/Lys-Trp-Glu/Asp-Leu/Ile-Ile-Ala-Gly-Arg-IleVal-Pro-Gly-Arg, where Xaa is any amino acid residue. An alternative exemplary consensus of the conserved domain is Val-Xaa-Met/Phe-Ser/Thr-Gln/Glu-Xaa-Glu-Glu-Asp-Leu-Val-Ser-Arg-Met-His-Arg-Leu-Val-Gly-Asn-Arg-Trp-Glu-Leu-Ile-Ala-Gly-Arg-Ile-Xaa-Gly-Arg, where Xaa is any amino acid residue. A further alternative exemplary consensus of the conserved domain is Val-Xaa-Met/Phe-Ser/Thr-Gln/Glu-Xaa-Glu-Glu-Asp-Leu-Val-Ser-Arg-Met-Tyr-Xaa-Leu-Val-Gly-Asn/Glu-Arg-Trp-Ser-Leu-Ile-Ala-Gly-Arg-Ile-Pro-Gly-Arg, where Xaa is any amino acid residue.

Potential Applications

The potential utility of this gene or its equivalogs is to confer heat tolerance to germinating seeds.

G682 or its equivalogs could be used to alter trichome number and distribution in plants. Trichome glands on the surface of many higher plants produce and secrete exudates, which give protection from the elements and pests such as insects, microbes and herbivores. These exudates may physically immobilize insects and spores, may be insecticidal or ant-microbial or they may allergens or irritants to protect against herbivores. Trichomes have also been suggested to decrease transpiration by decreasing leaf surface air flow, and by exuding chemicals that protect the leaf from the sun.

G715 (SEQ ID NO: 149)

Published Information

G715 is a member of the Hap5 subfamily of CCAAT-box transcription factors. G715 corresponds to Hap5a, and was found to be expressed ubiquitously in Arabidopsis (Edwards, et al. (1998) Plant Physiol. 117: 1015-1022).

Experimental Observations

The complete sequence of G715 was determined. The function of this gene was analyzed using transgenic plants in which G715 was expressed under the control of the 35S promoter. The expression of G715 appeared to be ubiquitous.

G715 overexpressors had higher seed oil content in the lines tested by NIR.

Potential Applications

G715 or its equivalogs could be used to increase seed oil content, which would be of nutritional value for food for human consumption as well as animal feeds.

G720 (SEQ ID NO: 151)

Published Information

G720 was described as APRR2, for Arabidopsis pseudo-response regulator (Makino et al. 2000 Plant Cell Physiol. 41:791-803). This designation reflects the fact that the protein contains significant homology to a receiver domain at the N-terminus, but has a glutamate in place of the conserved aspartate residue that is phosphorylated by a histidine kinase or phosphotransmitter protein.

Closely Related Genes from Other Species

G720 showed significant similarity to a drought-induced M. truncatula EST, GenBank accession number BG450227, that encodes a pseudo-receiver domain. The sequence similarity is high enough to suggest that the two proteins are orthologs, and the fact that G720 was also drought-induced is consistent with this hypothesis. Other ESTs from tomato and potato (BG642566, BG128919, BG129142, and BG887673) also showed high similarity to G720 and represent potential orthologs.

Experimental Observations

The complete sequence of G720 (SEQ ID NO: 151) was determined. A line homozygous for a T-DNA insertion in G720 and lines overexpressing G720 under the 35S promoter were used to determine the function of this gene. The T-DNA insertion in G720 was approximately half-way into the coding sequence, just before the conserved domain, and therefore should result in a null mutation. G720 knockout mutants were slightly more sensitive to freezing than the wild-type controls when the seedlings were cold-acclimated prior to freezing. G720 overexpressing lines were more tolerant to freezing. When seedlings were frozen at −10° C. for 20 hours, the G720 plants recovered better compared to the wild-type control in two separate experiments. G720 was induced by ABA, salt, osmotic stress, drought, heat, and auxin. The combination of enhanced sensitivity to freezing in the knockout mutants, enhanced resistance in the overexpressing lines, and the induction pattern of G720 comprised strong evidence that G720 functions in regulation of dehydration tolerance, as freezing is a form of dehydration stress.

Plants overexpressing G720 also showed reduced time to flowering in the T1 generation. One third of the 35S::G720 T1 seedlings, from each of two separate batches, flowered markedly earlier (up to 1 week sooner, 24-hour light conditions) than controls plants. All of the T1 lines showed high levels of G720 overexpression (determined by RT-PCR). Three early flowering T1 plants were selected for further study. However, none of these lines flowered early in the T2 generation, suggesting that activity of the transgene might have been reduced between the generations

Potential Applications

G720 or its equivalogs could be used to increase freezing tolerance in plants, and tolerance to other forms of moisture stress such as drought.

G736 (SEQ ID NO: 153)

Published Information

G736 was discovered as a full length EST clone. It was subsequently localized to BAC AC002341.

Experimental Observations

RT-PCR analysis of the endogenous levels of G736 indicated that this gene was expressed at low to medium levels in all tissues tested. In addition, there was no induction of G736 above its basal level in response to environmental stress treatments.

Two out of three G736 overexpressing lines exhibited a severe late flowering phenotype in both the T1 and T2 generation, the third line was late flowering in the T1 generation but the phenotype was lost in the subsequent generation, most likely due to silencing of the transgene. All three lines exhibited elongated petioles in both generations, and in two of the T1 lines, failure of the siliques to elongate was also observed. This phenotype was lost in the subsequent generation.

Potential Applications

Overexpression of G736 and its equivalog may be used to substantially delay flowering. A wide variety of applications exist for genes that either lengthen or shorten the time to flowering, or for systems of inducible flowering time control. In particular, in species where the vegetative parts of the plants constitute the crop and the reproductive tissues are discarded, it would be advantageous to delay or prevent flowering. Extending vegetative development could bring about large increases in yields. Additionally, a major concern is the escape of transgenic pollen from GMOs to wild species or so-called organic crops. Systems that prevent vegetative transgenic crops from flowering would eliminate this worry.

G748 (SEQ ID NO: 155)

Published Information

A cDNA sequence for G748 was deposited in GenBank by Abbaraju and Oliver on Aug. 4, 1998. G748 encodes a protein containing a D of zinc-finger domain that was found to bind the H-protein promoter. The H protein is a component of the glycine decarboxylase multienzyme complex, that comprises over one-third of the soluble proteins in mitochondria isolated from the leaves of C3 plants (Oliver et al. (1995) Bioenerg. Biomembr. 27: 407-414). A published function for G748 is a putative regulatory role in H-protein gene expression, suggested by the promoter-binding data.

Closely Related Genes from Other Species

Close relatives to G748 include a rice gene (GB accession # BAA88190) and a pumpkin gene (GB accession # D45066). In both cases, the similarity extends beyond the conserved DNA-binding domain, which suggests the genes could be orthologs of G748. The pumpkin gene encodes an ascorbate oxidase promoter-binding protein, suggesting that the product of G748 could also bind that promoter.

Experimental Observations

A cDNA sequence was isolated and used to produce transgenic plants overexpressing G748. Overexpression of G748 resulted in a late flowering phenotype. Transgenic plants were generally large and dark green with more rosette leaves. Stems were thicker and more vascular bundles were noticeable in transverse sections. G748 overexpressors also produced more lutein in seeds (consistently observed in three lines). The high lutein phenotype was confirmed in a repeat experiment. The physiology of the plant was similar to that of the controls. In wild-type plants, G748 was constitutively expressed, although at lower levels at the seedling stage. Expression levels were lower upon infection with E. orontii and Fusarium.

Potential Applications

Experimental data showed that G748 or its equivalogs can be used to delay flowering in transgenic plants.

Arabidopsis plants overexpressing G748 produced more lutein in seeds.

Plants transformed with G748 had modified stem morphology and vascular bundles and may be used to affect overall plant architecture.

G779 (SEQ ID NO: 157)

Published Information

G779 has been previously identified; fruits from a ind1 knockout mutant plants do not show cell differentiation in the dehiscence zone (Liljegren et al. (2000) Abstracts 11th Intl. Conf: Arabidopsis Res., Madison, Wis., pp. 179). These results suggest that G779 may mediate cell differentiation during Arabidopsis fruit development.

Closely Related Genes from Other Species

G779 is closely related to a Brassica rapa subsp. Pekinensis cDNA isolated from flower bud (acc#AT002234).

Experimental Observations

The function of G779 was analyzed using transgenic plants in which G779 was expressed under the control of the 35S promoter. Morphological analysis of overexpressors indicated that primary transformants of G779 had high levels of anthocyanin in seedlings, produced small plants with disorganized rosettes and short internodes, and many had flower abnormalities. The transformants with flower abnormalities showed conversion of sepals to carpels. The most severely affected had full conversion of sepals to carpels with ovules, stigmatic tissue on petals and stamens, and in some cases showed organ fusions. In the severe case of one T1 line, some inflorescences showed no flowers at all. Plants with a weak phenotype showed only small patches of stigmatic tissue on sepals. The floral phenotypes decreased acropetally. The plants showing the strongest phenotypes were essentially sterile, and did not produce T2 progeny for further analysis.

The phenotype produced by overexpressing G779 and G1499 was similar in the aspects of flower structures. Cluster analysis using basic helix-loop-helix motif revealed that both proteins of G779 and G1499 are closely related. The fact that expression of G779 was induced by auxin treatment in the rosette leaves indicates that G779 may play some kind of role in the auxin signal transduction pathway.

Potential Applications

G779 or its equivalogs could be used to modify plant architecture and development, including flower structure. If expressed under a flower-specific promoter, it might also be useful for engineering male sterility. Because expression of G779 is flower, embryo and silique specific, its promoter could be useful for targeted gene expression in these organs.

G789 (SEQ ID NO: 159)

Published Information

A partial sequence of G789 was identified from an EST clone (GenBank accession number T41998).

Experimental Observations

G789 was initially identified as a public EST (GenBank accession number T41998) and subsequently a full length library clone was identified. The function of G789 was analyzed using transgenic plants in which G789 was expressed under the control of the 35S promoter.

Overexpression of G789 reduced the time to flowering under continuous light conditions; this phenotype was most prevalent in the T2 generation and was noted in all three of the lines analyzed.

Transgenic plants overexpressing G789 were more sensitive to the herbicides glyphosate and acifluorfen and to oxidative stress caused by rose bengal compared to wild-type controls. Furthermore, G789 overexpressing lines were more susceptible to infection with Sclerotinia sclerotiorum when tested as mixed lines in two repeat experiments. This disease susceptibility phenotype did not repeat when individual lines were tested. It is well known that oxidative stress is a component of a plant defense response to pathogen and therefore, the disease susceptibility phenotype could thus be related to a general sensitivity to oxidative stress.

Based on the RT-PCR analysis, G789 was constitutively expressed in all tissues; its expression level was unaffected by any of the conditions tested.

Potential Applications

Based on the current analysis of G789 overexpressing plants, G789 or its equivalogs could be used to manipulate flowering time.

Since G789 activity has been shown to be required for the protection of Arabidopsis plants against oxidative stress, G789 or its equivalogs could be used to manipulate defenses against abiotic and biotic stresses such as disease, UV-B radiation, ozone pollution and herbicide application.

G801 (SEQ ID NO: 161)

Published Information

A partial sequence for G801 was identified from EST clones (GenBank accession numbers N97289, H36373 and Z32574).

Experimental Observations

G801 is a proprietary sequence initially identified as three partial public ESTs (GenBank accession numbers N97289, H36373 and Z32574). Subsequently, a full length library clone was identified. The function of G801 was analyzed using transgenic plants in which G801 was expressed under the control of the 35S promoter. Morphological analysis revealed that a minority of primary transformants of G801 were dark green and late flowering. However, T2 lines derived from three late-flowering lines showed no flowering time differences from control plants. Plant overexpressing G801 showed more seedling vigor when germinated on media containing high salt compared to wild-type control plants. All three overexpressing lines showed similar degrees of tolerance. In addition, overexpression of G801 in Arabidopsis resulted in an increase in seed oil content. This phenotype was observed in a single line.

Potential Applications

The potential utilities of this gene or its equivalogs include the ability to confer salt tolerance during the germination stage of a crop plant. This would most likely impact survivability and yield. Evaporation of water from the soil surface causes upward water movement and salt accumulation in the upper soil layer, where the seeds are placed. Thus, germination normally takes place at a salt concentration much higher than the mean salt concentration in the whole soil profile.

In addition, G801 or its equivalogs may be used to increase seed oil in crop plants.

G849 (SEQ ID NO ID NO: 163)

Published Information

The transcription factor G849 is an Arabidopsis homolog of parsley BPF-1, a pathogen inducible DNA-binding protein. BPF-1, Box-P Binding Factor 1, was reported by da Costa e Silva et al. ((1993) Plant Journal 4:125-135) to bind specifically to the P-box sequence motif of the phenylalanine ammonia lyase promoter, a key enzyme of the phenylpropanoid metabolism. G849 is found in the sequence of chromosome 3, BAC T2E22 (GenBank AC069474.4 GI:12321944), released by the Arabidopsis Genome Initiative. The start and stop codons were correctly predicted.

Experimental Observations

NIR analyses performed on G849 knockout plants revealed increased total combined seed oil and protein content.

RT-PCR analysis of the endogenous level of G849 transcripts revealed high constitutive expression in all tissues examined, with the exception of germinated seed. A detectable but low level of G849 transcripts was observed in germinated seeds. G849 transcript level increased significantly upon auxin, ABA, cold, heat and salt treatment, as well as seven days post-inoculation with Erysiphe orontii.

Potential Applications

Based on the knockout analyses, G849 or its equivalogs may be used to modify seed oil and protein content.

The null mutant of G849 had altered seed phytosterol composition, a decease in beta-sitosterol, as well as changes in leaf insoluble sugars. Phytosterols are an important source of precursors for the manufacture of human steroid hormones by semisynthesis. Sitosterols and stigmasterols, not campesterol, are the preferred sources from seed crops. Phytosterols and their hydrogenated derivatives phytostanols also have proven cholesterol-lowering properties.

G859 (SEQ ID NO: 165)

Published Information

G859 corresponds to MXK3.30 (BAB10332). The high level of sequence similarity between G859 and FLOWERING LOCUS C (FLC; Michaels et al. (1999) Plant Cell 11, 949-956; Sheldon et al., (1999) Plant Cell 11, 445-458) has been described previously (Ratcliffe et al. (2001) Plant Physiol. 126:122-132). G859 has also been referred to as AGL31 (Alvarez-Buylla et al. (2000) Plant J. 24:457-466).

Experimental Observations

G859 was recognized as a gene highly related to Arabidopsis FLC, and to MADS AFFECTING FLOWERING 1. FLC acts as a repressor of flowering (Michaels (1999) Plant Cell 11, 949-956; Sheldon et al. (1999) Plant Cell 11, 445-458). Similarly, G157/MAF1 can cause a delay in flowering time when overexpressed (Ratcliffe et al. (2001) Plant Physiol. 126:122-132).

The function of G859 was studied using transgenic plants in which this gene was expressed under the control of the 35S promoter. Overexpression of G859 modified the timing of flowering, with very high levels of G859 activity delaying the floral transition in the Columbia ecotype. No alterations were detected in 35S::G859 plants in the physiological and biochemical analyses that were performed.

Under continuous light conditions, the majority of 35S::G859 primary transformants (overexpressing a construct containing a full-length cDNA, P1688) were earlier flowering than wild-type controls. This result was observed in multiple independent batches of T1 plants and in either continuous or 12 hour light conditions. However, in each selection of primary transformants, a small number of lines were late flowering. RT-PCR analyses demonstrated that all T1 plants overexpressed the transgene, but that the highest levels of expression were found in the late flowering transformants. Comparable results were also obtained when plants were transformed with a construct (P376) containing a shorter splice-variant of G859. The effects on flowering time caused by overexpression of G859, and the dependence of those effects on the transgene expression levels, mirror results previously obtained for G157/MAF1 (Ratcliffe et al. (2001) Plant Physiol. 126:122-132).

Seed was taken for T2 analyses from two late flowering primary transformants, and a T1 plant that had been early flowering. The progeny of the former two lines all appeared markedly late flowering, while the T2 plants from the third line were marginally late flowering. No convincing early flowering was observed in any the three T2 populations. Thus, in the second generation, the predominant effect of G859 activity was delayed flowering. In a follow-up experiment it was found that late flowering 35S::G859 T2 plants were photoperiod responsive, and were not sensitive to extensive vernalization treatments.

Potential Applications

G859 or its equivalogs could be used to alter flowering time.

G864 (SEQ ID NO ID NO: 167)

Published Information

G864 was identified in an Arabidopsis EST (H37693). G864 appears as gene AT4g23750 in the annotated sequence of Arabidopsis chromosome 4 (AL161560).

Experimental Observations

G864 was discovered and initially identified as a public Arabidopsis EST.

The complete sequence of G864 was determined, and G864 was found to be related to two additional Arabidopsis AP2/EREBP genes, G1421 and G1755. The function of G864 was analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter. G864 overexpressing plants exhibited a variety of phenotypic alterations. They were smaller than wild-type plants, and those with the strongest phenotypes were classified as dwarf. However, G864 overexpressing lines showed more seedling vigor in a heat stress tolerance germination assay compared to wild-type controls. Conversely, G864 overexpressing lines were also somewhat more sensitive to chilling. One of the three T2 lines analyzed showed significant increase in fucose and arabinose levels in leaves.

G864 was ubiquitously expressed, and was not significantly induced under any of the conditions tested.

Potential Applications

The germination of many crops is very sensitive to temperature. A gene that would enhance germination in hot conditions such as G864 or its equivalogs would be useful for crops that are planted late in the season or in hot climates.

G867 (SEQ ID NO: 169)

Published Information

G867 corresponds to RAV1 (Kagaya et al. (1999) Nucleic Acids Res. 27: 470-478). G867/RAV1 belongs to a small subgroup within the AP2/EREBP family of transcription factors, whose distinguishing characteristic is that its members contain a second DNA-binding domain, in addition to the conserved AP2 domain, that is related to the B3 domain of VP1/ABI3 (Kagaya et al. (1999) supra). It has been shown that the two DNA-binding domains of RAV1 can separately recognize each of two motifs that constitute a bipartite binding sequence and together cooperatively enhance its DNA-binding affinity and specificity (Kagaya et al. (1999) supra).

Experimental Observations

G867 was discovered and initially identified as a public Arabidopsis EST. G867 appeared to be constitutively expressed at medium levels.

G867 was first characterized using a line that contained a T-DNA insertion in the gene. The insertion in that line resided immediately downstream of the conserved AP2 domain, and would therefore be expected to result in a severe or null mutation. G867 knockout mutant plants did not show significant changes in overall plant morphology, significant differences between these plants and control plants have not been detected in any of the assays that have been performed so far.

Subsequently, the function of G867 was analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter. G867 overexpressing lines were morphologically wild-type and no phenotypic alterations in G867 overexpressing lines were detected in the biochemical assays that were performed. However, G867 overexpressing lines showed increased seedling vigor (manifested by increased expansion of the cotyledons) in germination assays on both high salt and high sucrose containing media, compared to wild-type controls.

The Arabidopsis paralogs G1930 (SEQ ID NO: 369) and G9 (SEQ ID NO: 1949) also showed stress related phenotypes. G9 exhibited increased root biomass, and thus could be used to produce better plant growth under adverse osmotic conditions. Genetic and physiological evidence indicates that roots subjected to various stresses, including water deficit, alter the export of specific compounds, such as ACC and ABA, to the shoot, via the xylem Bradford et al. (1980) Plant Physiol. 65: 322-326; Schurr et al. (1992) Plant Cell Environ. 15, 561-567).

G1930 plants responded to high NaCl and high sucrose on plates with more seedling vigor, and root biomass compared to wild-type control plants; this phenotype was identical to that seen in 35S::G867 lines. These results indicate a general involvement of this clade in abiotic stress responses:

The polypeptide sequences of G1930 and G9 share 72% (249/345 residues) and 64% (233/364 residues) with G867, respectively. The conserved domains of G1930 and G9 are 86% (56/65 residues) and 86% (56/65 residues) identical with the conserved domain of G867, respectively.

In addition to the paralogous sequences disclosed above, orthologous sequences from other plant species were also identified using BLAST analysis. Such orthologous sequences, together with the paralogous sequences were determined to be members of the G867 TF family of AP2 proteins (equivalogs). The paralogous sequences and the orthologous sequences were aligned using MACVECTOR software (Accelrys, Inc.). The software program also generated an exemplary consensus amino acid residue sequence of the aligned sequences.

As shown in FIGS. 4A, 4B, 4C, and 4D, the orthologous sequences shared a consensus sequence with the conserved domain of G867 (amino acid residues 59-116 of SEQ ID NO:170) and also shared identity with regions flanking the conserved domain (flanking regions). In particular, G867 shared a region of the conserved domain with sequences from soy (Glycine max; SEQ ID NOs: 1184, 1183, and 1182), rice (Oryza sativa; SEQ ID NOs: 1176, 1177, and 1178), and maize (corn) (Zea mays; SEQ ID NOs: 1186 and 1185).

An exemplary consensus of the conserved domain of the G867 TF family of AP2 proteins is Ser-Ser-Lys/Arg-Tyr/Phe-Gly-Val-Val-Pro-Gln-Pro-Asn-Gly-Arg-Typ-Gly-Ala-Gln-Ile-Tyr-Glu-Lys/Arg-His-Gln-Arg-Val-Trp-Leu-Gly-Thr-Phe-Xaa-Glu/Asp-Glu-Glu/Asp-Glu/Asp-Ala-AlaVal-Arg-Ala/Ser-Tyr-Asp-Val/Ile-AlaVal-Val/Ala-Xaa-Arg-Phe/Tyr-Arg-Arg/Gly-Arg-Asp-Ala-Val-Thr/Val-Asn-Phe-Lys/Arg, where Xaa is any amino acid residue. An alternative exemplary consensus of the conserved domain is Ser-Ser-Lys/Arg-Tyr/Phe-Gly-Val-Val-Pro-Gln-Pro-Asn-Gly-Arg-Typ-Gly-Ala-Gln-Ile-Tyr-Glu-Lys/Arg-His-Gln-Arg-Val-Trp-Leu-Gly-Thr-Phe-Xaa-Glu/Asp-Glu-Glu/Asp-Ala-Ala-Ala-Arg-Ala-Tyr-Asp-Val/Ile-Ala Val-Val/Ala-Xaa-Arg-Phe/Tyr-Arg-Arg/Gly-Arg-Asp-Ala-Val-Thr/Val-Asn, where Xaa is any amino acid residue. A further alternative exemplary consensus of the conserved domain is Ser-Ser-Lys/Arg-Tyr/Phe-Gly-Val-Val-Pro-Gln-Pro-Asn-Gly-Arg-Typ-Gly-Ala-Gln-Ile-Tyr-Glu-Lys/Arg-His-Gln-Arg-Val-Trp-Leu-Gly-Thr-Phe-Xaa-Gly-Glu-Ala/Asp-Glu/Asp-Ala-Ala/Val-Arg-Ala-Tyr-Asp-Val-Ala-Ala-Gln-Arg-Phe/Tyr-Arg-Arg/Gly-Arg-Asp-Ala-Val-Thr/Val-Asn-Phe-Arg, where Xaa is any amino acid residue.

Potential Applications

G867 or its equivalogs could be used to increase or facilitate seed germination and seedling growth under adverse environmental conditions, in particular salt stress.

G867 or its equivalogs may also be used to modify sugar sensing.

G869 (SEQ ID NO: 171)

Published Information

A partial cDNA sequence of G869 is available as public ESTs N65486. The sequence of G869 later appeared among the Arabidopsis sequences released by the Arabidopsis Genome Initiative, in BAC T26J14 (GenBank accession number AC011915).

Experimental Observations

The complete cDNA sequence of G869 was determined. The function of this gene was analyzed using transgenic plants in which G869 was expressed under the control of the 35S promoter. Plants overexpressing G869 were small with spindly bolts. G869 transgenic plants showed alterations in leaf and seed fatty acid composition. In leaves, 16:0 levels decreased and 16:3 levels increased. These changes likely reflected alterations in the desaturation state of chloroplast membranes. In seeds, 18:1 levels increased significantly. The increase in the seed 18:1 fatty acid in two lines was observed in a repeat experiment. A decrease in 18:3 and 20:0 was also noted in these lines.

Alterations in the levels of leaf insoluble sugars were also detected, with the increase in fucose determined to be significant. In addition, G869 overexpressors were more tolerant to infection with a moderate dose of the fungal pathogen Erysiphe orontii. The increase in resistance phenotype co-segregated with the dwarf phenotype. G869 plants showed additional morphological alterations, including poor fertility due to underdeveloped anthers.

Potential Applications

G869 or its equivalogs could be useful to manipulate the saturation levels of lipids in seeds. Alteration in seed lipid saturation could be used to improve the heat stability of oils or to improve the nutritional quality of seed oil.

As G869 transgenic plants have an altered response to the fungal pathogen Erysiphe orontii, G869 or its equivalogs could be used to manipulate the defense response in order to generate pathogen-resistant plants.

G877 (SEQ ID NO: 173)

Published Information

G877 was identified in an Arabidopsis EST (37131). G877 is contained in P1 clone MXK23 (GenBank accession number AB026656).

Closely Related Genes from Other Species

A non-Arabidopsis gene closely related to G877 is the tobacco gene NtWRKY4 (GenBank accession number AB026890). Similarity between these two genes extends beyond the conserved WRKY domain.

Experimental Observations

G877 was first discovered and identified as a public Arabidopsis EST. The complete sequence of G877 was determined.

A line was identified that contains a T-DNA insertion in the coding sequence of G877. The insertion likely resulted in a null mutation, since it resided upstream of the conserved WRKY domain sequence. Plants that were hemizygous for that insertion segregate 3 viable:1 inviable seeds in the silique, and homozygous G877 knockout mutant plants were never obtained. Therefore, a (null) mutation in G877 results in embryo lethality.

G877 was ubiquitously expressed. G877 is likely to be involved in controlling some essential process(es) required for growth rather than specific aspects of embryo patterning and development. Alternatively, G877 might play different roles throughout the plant life cycle.

Potential Applications

The embryo lethal phenotype of a G877 mutation indicates that the gene is involved in the control of some essential aspect of growth and development. G877 or its equivalogs could therefore constitute an herbicide target, either by itself or by allowing the identification of other genes or processes essential for plant growth.

G881 (SEQ ID NO ID NO: 175)

Published Information

G881 corresponds to gene F28M20.10, first identified in the sequence of BAC clone F28M20 (released by the Arabidopsis Genome Initiative; GenBank accession number AL031004).

Experimental Observations

The complete cDNA sequence for G881 was determined. The annotation in GenBank for this gene (BAC AL031004) was found to be inaccurate. G881 was ubiquitously expressed, but appeared to be significantly induced in response to salicylic acid treatment. The function of this gene was analyzed using transgenic plants in which G881 was expressed under the control of the 35S promoter. G881 overexpressors appeared to be more susceptible to infection with a moderate dose of the fungal pathogen Erysiphe orontii. Increased susceptibility to Erysiphe orontii was confirmed in repeat experiment. The induction of G881 expression by SA also implicated G881 in the disease response.

Potential Applications

Since G881 transgenic plants appear to have an altered response to the fungal pathogen Erysiphe orontii, G881 or its equivalogs could be used to manipulate the defense response in order to generate pathogen-resistant plants.

G892 (SEQ ID NO: 177)

Published Information

G892 was identified in the sequence of BAC clone T13D8, GenBank accession number AC004473, released by the Arabidopsis Genome Initiative.

Experimental Observations

The complete sequence of G892 was determined. A line homozygous for a T-DNA insertion in G892 was used to determine the function of this gene. The T-DNA insertion of G892 was approximately 70% into the coding sequence of the gene and therefore was likely to result in a null mutation. The phenotype of these transgenic plants was wild-type in all assays performed. G892 appeared to be constitutively expressed at low or moderate levels in all tissues except in roots where expression was much higher. ABA or salt treatment caused a slight increase in the expression of G892.

G892 knockout mutants were found to have increased seed oil and decreased protein content compared to wild-type plants.

Potential Applications

G892 or its equivalogs may be used to alter seed oil and protein content in plants, which may be very important for the nutritional value and production of various food products.

G896 (SEQ ID NO: 179)

Closely Related Genes from Other Species

G896 is very similar to a peppermint EST (AW255156). Since the homology extends beyond the conserved domain, G896 and the mint gene are likely orthologs.

Experimental Observations

A knock-out mutant was isolated, which contains a T-DNA insertion 40 base pairs downstream of the start codon. G896 knock-out plants were more susceptible to Fusarium oxysporum. In addition, G896 knockout plants had lower levels of lutein in seeds as compared to wild-type control plants. Otherwise, the knock-out plants had a wild-type morphological phenotype.

In wild-type plants, G896 was mostly expressed in roots. Changes in environmental conditions did not affect its expression.

Potential Applications

Since G896 transgenic plants have an altered response to the fungal pathogen Fusarium oxysporum, the gene or its equivalogs could be used to manipulate the defense response in order to generate pathogen-resistant plants.

G910 (SEQ ID NO: 181)

Published Information

G910 was identified as a gene in the sequence of BAC T22E19 (Accession Number AC016447), released by The Institute for Genomic Research.

Experimental Observations

The function of G910 was analyzed using transgenic plants in which G910 was expressed under the control of the 35S promoter.

G910 overexpression produced pleiotropic effects on plant development, but the most marked result was a delay in the transition to flowering. At early stages, 35S::G910 T1 lines appeared normal, but by around 20 days after sowing, most plants were clearly smaller than wild type and often had contorted or serrated leaves with short petioles. Approximately half of the T1 lines bolted at a normal time and produced rather thin inflorescences that yielded relatively few seeds. The remaining half of the T1 lines typically produced flowers between five and 30 days later than wild type. Although such late flowering plants initially appeared small, in many cases, by the time of bolting, they had attained a similar size and produced a much larger number of primary rosette leaves than controls. Two T2 lines showed a similar, but more extreme late flowering phenotype.

G910 was expressed at low levels in shoots and germinating seedlings, and at higher levels in flowers, rosette leaves, embryonic tissue and siliques. The expression of G910 was repressed by cold treatment and induced by NaCl treatment.

Potential Applications

Plants expressing G910 exhibited a delayed flowering time relative to controls. A wide variety of applications exist for genes or their equivalogs that either lengthen or shorten the time to flowering.

G911 (SEQ ID NO: 183)

Closely Related Genes from Other Species An EST (GenBank accession AI352907) induced in the defense response of Brassica napus to Leptosphaeria maculans has extremely high homology both within and external to the conserved RING H2 domain.

Experimental Observations

The function of G911 was analyzed through its ectopic overexpression in Arabidopsis. RT-PCR of endogenous levels of G911 indicated this gene was expressed in all tissues tested. A cDNA array experiment confirmed this tissue distribution data by RT-PCR. Microarray data confirmed that G911 was overexpressed 23 fold. Other genes that were induced when G911 was overexpressed included RHA1b (another RING C2H3C2 transcription factor), pistilata, and a proline rich protein isolog. Plants overexpressing G911 looked healthier and had longer roots when grown on media lacking potassium compared to wild-type plants.

Potential Applications

Plants overexpressing G911 or its equivalogs may be able to be grown with fertilizer lacking or containing low potassium.

G912 (SEQ ID NO: 185)

Published Information

G912 was identified in the sequence of P1 clone MSG15 (GenBank accession number AB015478; gene MSG15.6).

Closely Related Genes from Other Species

G912 is closely related to CBF1, CBF2, and CBF3, and also closely related to the members of the CBF-like subgroup of AP2/EREBP proteins from other plants, like AF084185 Brassica napus dehydration responsive element binding protein.

Experimental Observations

G912 was recognized as the AP2/EREBP gene most closely related to Arabidopsis CBF1, CBF2, and CBF3 (Stockinger et al (1997) Proc. Natl. Acad. Sci. USA 94:1035-1040; Gilmour et al. (1998) Plant J. 16:433-442). In fact, G912 is the only other AP2/EREBP transcription factor for which sequence similarity with CBF1, CBF 2, and CBF3 extends beyond the conserved AP2 domain.

The function of G912 was studied using transgenic plants in which this gene was expressed under the control of the 35S promoter. Plants overexpressing G912 were more freezing and drought tolerant than the wild-type controls, but were also small, dark green, and late flowering. There was a positive correlation between the degree of growth impairment and the freezing tolerance. In addition, G912 expression appeared to be induced by cold, drought, and osmotic stress.

In addition, G912 overexpressing plants also exhibited a sugar sensing phenotype: reduced seedling vigor and cotyledon expansion upon germination on high glucose media.

These results mirror the extensive body of work that has shown that CBF1, CBF2, and CBF3 are involved in the control of the low-temperature response in Arabidopsis, and that those genes can be used to improve freezing, drought, and salt tolerance in plants (Stockinger et al., (1997) Proc. Natl. Acad. Sci. USA 94:1035-1040; Gilmour et al. (1998) Plant J. 16:433-442; Jaglo-Ottosen et al. (1998) Science. 280:104-106; Liu et al. (1998) Plant Cell. 10:1391-1406, Kasuga et al. (1999) Nat. Biotechnol. 17:287-291).

The polypeptide sequences of G40, G41, and G42 share 71% (140 of 195 residues), 68% (144 of 211 residues), and 65% (147 of 224 residues) identity with G912, respectively. The conserved domains of G40, G41, and G42 share 94% (64 of 68 residues), 92% (63 of 68 residues), and 94% (64 of 68 residues) identity with G912, respectively.

In addition to the paralogous sequences disclosed above, orthologous sequences from other plant species were also identified using BLAST analysis. Such orthologous sequences, together with the paralogous sequences were determined to be members of the G912 TF family of AP2/EREBP proteins (equivalogs). The paralogous sequences and the orthologous sequences were aligned using MACVECTOR software (Accelrys, Inc.). The software program also generated an exemplary consensus amino acid residue sequence of the aligned sequences.

As shown in FIGS. 5A, 5B, 5C, 5D, 5E, and 5F, the orthologous sequences shared a consensus sequence with the conserved domain of G912 (amino acid residues 51-118 of SEQ ID NO:186) and also shared identity with regions flanking the conserved domain (flanking regions). In particular, G912 shared a region of the conserved domain with sequences from soy (Glycine max; SEQ ID NOs: 1238, 1242, 1240, 1241, and 1243), rice (Oryza sativa; SEQ ID NOs: 1222, 1223, 1232, 1221, 1231, 1227, 1235, 1230, 1229, and 1228), and maize (corn) (Zea mays; SEQ ID NOs: 1246, 1247, 1244, and 1245).

An exemplary consensus of the conserved domain of the G912 TF family of AP2/EREBP proteins is His-Pro-Ile/Val-Tyr/Phe-Arg/Lys-Gly-Val-Arg-Gln/Arg-Arg-Gly/Asn-Xaa₍₁₋₃₎-Lys/Arg-Trp-Val-Cys/Ser-Glu-Val/Leu-Arg-Glu/Val-Pro-Asn-Lys-Xaa₍₂₎-Arg-Ile/Leu-Trp-Leu-Gly-Thr-Phe/Tyr-Xaa₍₂₎-Ala/Pro-Glu-Met-Ala-Ala-Arg-Ala-His-Asp-Val-Ala-Ala/Met-Leu/Met-Ala-Leu-Arg-Gly-Xaa₍₁₋₈₎-Ala-Cys-Leu-Asn-Phe-Ala-Asp-Ser-Xaa₍₁₋₅₎-Val/Ile-Pro/Asp, where Xaa is any amino acid residue. An alternative exemplary consensus of the conserved domain is His-Pro-Ile/Val-Tyr/Phe-Arg/Lys-Gly-Val-Arg-Xaa-Arg-Gly/Asn-Xaa₍₁₋₃₎-Lys/Arg-Trp-Val-Cys/Ser-Glu-Val/Leu-Arg-Glu/Val-Pro-Xaa₍₁₋₅₎-Arg-Ile/Leu/Phe-Trp-Leu-Gly-Thr-Phe/Tyr-Xaa₍₂₎-Ala/Pro-Glu-Xaa-Ala-Ala-Arg-Ala-His-Asp-Val-Ala-Ala/Met-Leu/Met-Ala-Leu-Arg-Gly-Xaa₍₁₋₈₎-Ala-Cys/Ser-Leu-Asn-Phe-Ala-Asp-Ser-Xaa₍₁₋₅₎-Val/Ile-Pro/Asp, where Xaa is any amino acid residue.

An exemplary flanking region consensus sequence of the G912 TF family of AP2/EREBP proteins is Pro-Lys-Xaa-Xaa-Ala-Gly-Arg (amino acids 37-43 of SEQ ID NO: 186), or Ala-Gly-Arg-Xaa-Lys-Phe (amino acids 41-46 of SEQ ID NO: 186) or Glu-Thr-Arg-His-Pro (amino acids 48-52 of SEQ ID NO: 186), where Xaa is any amino acid residue.

Potential Applications

G912 or its equivalogs could be used to improve plant tolerance to cold, freezing, drought, and salt stress. In addition, G912 or its equivalogs could be used to change a plant's flowering time and size.

G913 (SEQ ID NO: 187)

Published Information

G913 was identified in the sequence of clone MSG15; it corresponds to gene MSG15.10 (GenBank PID BAB11050).

Closely Related Genes from Other Species

G913 is highly similar to a Brassica napus protein, encoded by a gene represented by EST AI352878 MB72-11D PZ204.BNlib Brassica napus cDNA clone pMB72-11D 5′.

Experimental Observations

The cDNA sequence of G913 was determined. To investigate the function(s) of G913, this gene was expressed under the control of the 35S promoter in transgenic plants. G913 overexpressing plants had dark green leaves that occasionally curled downward. These plants showed a delay in flowering and were also late senescing. Overexpressing G913 lines were more freezing tolerant and more drought tolerant than the wild-type controls.

In an ethylene sensitivity assay where the plants were tested for a triple response phenotype on plates containing ACC, G913 overexpressing plants showed stunting and curling in the hypocotyl region that was more exaggerated than the wild type triple response.

Potential Applications

G913 or its equivalogs could be used to improve plant tolerance to freezing and drought. G913 could also be used to manipulate the ethylene response.

G913 or its equivalogs may be used to delay flowering or senescence in plants. Extending vegetative development could bring about large increases in yields.

Additionally, a major concern is the escape of transgenic pollen from GMOs to wild species or so-called organic crops. Systems that prevent vegetative transgenic crops from flowering would eliminate this worry.

G922 (SEQ ID NO: 189)

Published Information

G922 corresponds to Scarecrow-like 3 (SCL3) first described by Pysh et al. (GenBank accession number AF036301; (1999) Plant J. 18: 111-119). Northern blot analysis results show that G922 is expressed in siliques, roots, and to a lesser extent in shoot tissue from 14 day old seedlings. Pysh et al did not test any other tissues for G922 expression. In situ hybridization results showed that G922 was expressed predominantly in the endodermis in the root tissue. This pattern of expression was very similar to that of SCARECROW (SCR), G306. Experimental evidence indicated that the co-localization of the expression is not due to cross-hybridization of the G922 probe with G306. Pysh et al proposed that G922 may play a role in epidermal cell specification and that G922 may either regulate or be regulated by G306.

The sequence for G922 can also be found in the annotated BAC clone F11F12 from chromosome 1 (GenBank accession number AC012561). The sequence for F11F12 was submitted to GenBank by the DNA Sequencing and Technology Center at Stanford University.

Closely Related Genes from Other Species

The amino acid sequence for a region of the Oryza sativa chromosome I clone P0466H10 (GenBank accession number AP003259) is significantly identical to G922 outside of the SCR conserved domains. Therefore, the gene represented by this region of the rice clone may be the ortholog of G922.

Experimental Observations

The function of this gene was analyzed using transgenic plants in which G922 was expressed under the control of the 35S promoter. Transgenic plants overexpressing G922 were more salt tolerant than wild-type plants as determined by a root growth assay on MS media supplemented with 150 mM NaCl. Plant overexpressing G922 also were more tolerant to osmotic stress as determined by germination assays in salt-containing (150 mM NaCl) and sucrose-containing (9.4%) media. Morphologically, plants overexpressing G922 had altered leaf morphology, coloration, fertility, and overall plant size. In wild-type plants, expression of G922 was induced by auxin, ABA, heat, and drought treatments. In non-induced wild-type plants, G922 was expressed constitutively at low levels.

Potential Applications

Based upon results observed in plants overexpressing G922, G922 or its equivalogs could be used to alter salt tolerance, tolerance to osmotic stress, and leaf morphology in other plant species. Evaporation from the soil surface causes upward water movement and salt accumulation in the upper soil layer where the seeds are placed. Thus, germination normally takes place at a salt concentration much higher than the mean salt concentration of in the whole soil profile. Increased salt tolerance during the germination stage of a crop plant would impact survivability and yield.

Altered leaf morphology could be desirable in ornamental horticulture.

G926 (SEQ ID NO: 191)

Published Information

G926 is equivalent to Hap2a (Y13720), a member of the CCAAT-box binding transcription factor family. The gene was identified by Edwards et al. ((1998) Plant Physiol. 117: 1015-1022), who demonstrated that G926 or AtHap2a were able to functionally complement a Hap2 deficient mutant of yeast suggesting that there is functional conservation between these proteins from diverse organisms. In addition, the AtHap2a gene was shown to be ubiquitously expressed in Arabidopsis.

Closely Related Genes from Other Species

G926 is most closely related to a Brassica napus protein (AAC49265). Similarity between the two proteins extend beyond the signature motif of the family to a level that would indicate the genes are orthologous. No functional information is available for the Brassica napus protein.

Experimental Observations

Consistent with the published expression pattern (Edwards et al. (1998) Plant Physiol. 117: 1015-1022), G926 was determined to be ubiquitously expressed and transcript levels appeared to be unaltered by any environmental stress-related condition tested. A line homozygous for a T-DNA insertion in G926 was used to determine the function of this gene.

The G926 knockout mutant line was morphologically wild-type. Physiological analysis revealed that in the presumed absence of G926 function, the plants became more tolerant to high osmotic conditions during germination. This osmotic stress tolerance could be related to the plant's apparent insensitivity to the growth hormone ABA. This was the second instance where a member of a CCAAT-box protein complex altered the plants osmotic stress response and ABA sensitivity during germination.

ABA plays an important regulatory role in the initiation and maintenance of seed dormancy. Lopez-Molina, L. et al. ((2001) Proc. Natl. Acad. Sci. USA 98: 4782-4787) describe a bZIP transcription factor, ABI5, that is involved in maintaining seeds in a quiescent state, preventing germination under adverse conditions such as drought stress. It is possible G926 also functions as part of this checkpoint for the germinating seeds and loss of G926 function promotes germination regardless of the osmotic status of the environment.

Potential Applications

G926 or its equivalogs could be used to improve plant tolerance to drought, and salt stress.

Evaporation from the soil surface causes upward water movement and salt accumulation in the upper soil layer where the seeds are placed. Thus, germination normally takes place at a salt concentration much higher than the mean salt concentration of in the whole soil profile. Increased salt tolerance during the germination stage of a crop plant would impact survivability and yield.

G961 (SEQ ID NO: 193)

Published Information

G961 was first identified in the sequence of the BAC clone F19D11, GenBank accession number AC005310, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

The most related gene to G961 is a rice gene in accession number BAA84803.

Experimental Observations

The full length sequence of G961 was experimentally confirmed. The function of this gene was analyzed by knockout analysis. Plants homozygous for a T-DNA insertion in G961 were wild-type for all assays performed.

Gene expression profiling by RT-PCR showed that G961 was primarily expressed in shoots, embryos and siliques at medium levels, and at low levels in flowers. RT-PCR data also indicated an induction of G961 transcripts accumulation upon heat treatment.

G961 knockout mutants were found to have altered seed oil content as compared to wild-type plants.

Potential Applications

G961 or its equivalog knockout mutants may be used to alter seed oil content in plants, which may be very important for the nutritional value and production of various food products.

G971 (SEQ ID NO: 195)

Published Information

G971 corresponds to gene F28P10.30 (CAB41085).

Experimental Observations

The function of G971 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter.

Overexpression of G971 produced a marked delay in the transition to flowering. The effect was noted, to varying extents, in approximately half of the 35S::G971 primary transformants. These plants flowered between one and three weeks later than controls under continuous light conditions. At later stages, most of the plants also appeared darker green and developed larger leaves than controls. Two of the three T2 populations selected for further study displayed a comparable, but rather more extreme late flowering phenotype to that seen in the parental plants. At early stages, seedlings from these two lines were relatively small, but recovered as development progressed, and eventually became larger than wild type. No alterations were detected in 35S::G971 plants in the physiological and biochemical analyses that were performed.

G971 was ubiquitously expressed and does not appear to be significantly induced by any of the conditions tested.

Potential Applications

G971 or its equivalogs could be used to modify flowering time characteristics. A wide variety of applications exist for systems that either lengthen or shorten the time to flowering.

In species such as sugarbeet where the vegetative parts of the plants constitute the crop and the reproductive tissues are discarded, it would be advantageous to delay or prevent flowering. Extending vegetative development could bring about large increases in yields.

G974 (SEQ ID NO ID NO: 197)

Published Information

G974 was first identified in a BAC-end sequence (B28553; partial G974 sequence). G974 corresponds to gene F16L1.8 (BAC F16L1, AC024228).

Closely Related Genes from Other Species

Several AP2 proteins from a variety of species (Atriplex hortensis, Lycopersicon esculentum, Glycine max, Populus balsamifera, Medicago truncatula) exhibited sequence similarity with G974 outside of the signature AP2 domain sequence, and bear nearly identical AP2 domains. These proteins may be related.

Experimental Observations

The complete sequence of G974 (SEQ ID NO: 197) was obtained and G974 was studied using transgenic plants in which G974 was expressed under the control of the 35S promoter. Constitutive expression of G974 produced deleterious effects: the majority of 35S::G974 primary transformants showed a reduction in overall size and developed rather slowly compared to wild-type controls. These phenotypic alterations were not observed in the T2 generation, perhaps indicating silencing of the transgene. The T2 plants were wild-type in the physiological and biochemical analyses performed. G974 was ubiquitously expressed.

35S::G974 overexpressors had altered seed oil content.

Potential Applications

G974 or its equivalogs may be used to alter seed oil content in plants, which may be very important for the nutritional value and production of various food products.

G975 (SEQ ID NO: 199)

Published Information

G975 has appeared in the sequences released by the Arabidopsis Genome Initiative (BAC F9L1, GenBank accession number AC007591).

Closely Related Genes from Other Species

The non-Arabidopsis gene most highly related to G975 is represented by L46408 BNAF1258 Mustard flower buds Brassica rapa cDNA clone F1258. The similarity between G975 and the Brassica rapa gene represented by EST L46408 extends beyond the conserved AP2 domain that characterizes the AP2/EREBP family. This Brassica rapa gene appeared to be more closely related to G975 than Arabidopsis G1387, indicating that EST L46408 may represent a true G975 ortholog. The similarity between G975 and Arabidopsis G1387 also extends beyond the conserved AP2 domain.

Experimental Observations

G975 (SEQ ID NO ID NO: 199) was identified as a new member of the AP2/EREBP family (EREBP subfamily) of transcription factors. G975 was expressed in flowers and, at lower levels, in shoots, leaves, and siliques. GC-FID and GC-MS analyses of leaves from G975 overexpressing plants showed that the levels of C29, C31, and C33 alkanes were substantially increased (up to ten-fold) compared with control plants. A number of additional compounds of similar molecular weight, presumably also wax components, also accumulated to significantly higher levels in G975 overexpressing plants. C29 alkanes constituted close to 50% of the wax content in wild-type plants (Millar et al. (1998) Plant Cell 11:1889-1902), suggesting that a major increase in total wax content occurred in the G975 transgenic plants. However, the transgenic plants had an almost normal phenotype (although small morphological differences were detected in leaf appearance), indicating that overexpression of G975 was not deleterious to the plant. Overexpression of G975 did not cause the dramatic alterations in plant morphology that had been reported for Arabidopsis plants in which the FATTY ACID ELONGATION1 gene was overexpressed (Millar et al. 1998, Plant Cell 11:1889-1902). G975 may regulate the expression of some of the genes involved in wax metabolism. One Arabidopsis AP2 sequence (G1387) that is significantly more closely related to G975 than the rest of the members of the AP2/EREBP family is predicted to have a function and a use related to that of G975.

Potential Applications

G975 or its equivalogs can be used to manipulate wax composition, amount, or distribution, which in turn can modify plant tolerance to drought and/or low humidity or resistance to insects, as well as plant appearance (shiny leaves).

G975 or its equivalogs can also be used to specifically alter wax composition, amount, or distribution in those plants and crops from which wax is a valuable product.

G979 (SEQ ID NO: 201)

Published Information

G979 was first identified in a BAC-end sequence (B25031; partial G979 sequence). G979 corresponds to gene T12E18_(—)20 (BAC T12E18, AL132971). No information is available about the function(s) of G979.

Experimental Observations

The complete sequence of G979 was obtained. The function of this gene was studied using both transgenic plants in which G979 was expressed under the control of the 35S promoter (April 2001), and a line with a T-DNA insertion in the gene. G979 codes for an AP2 protein of the AP2 subfamily, i.e., it contains two AP2 domains. The T-DNA insertion of the KO line lies in an intron, located in between the exons coding for the second AP2 domain of the protein, and is thus expected to result in a strong or null mutation. Whereas constitutive expression of G979 produced deleterious effects, the analysis of G979 KO mutant plants proved informative about the function of the gene. It was suggested that proteins of the AP2 subfamily were more likely to be involved in developmental processes (Riechmann et al. (1998). Biol. Chem. 379: 633-646). Fittingly, seeds homozygous for a T-DNA insertion within G979 showed delayed ripening, slow germination, and developed into small, poorly fertile plants, indicating that G979 is involved in seed development processes.

The difficulty in initially isolating, from heterozygous plants, progeny that was homozygous for the T-DNA insertion raised the possibility that homozygosity for that allele was lethal. Siliques of heterozygous plants were examined for seed abnormalities. Approximately 25% of the seeds contained in young green siliques were pale in coloration. In older, brown siliques, approximately 25% of the seeds were green and appeared slow ripening, whereas the remaining seeds were brown. Thus, it seemed likely that the seeds with altered development were homozygous for the T-DNA insertion, whereas the normal seeds were wild-type and heterozygous segregants.

Furthermore, it was observed that approximately 25% of the seed from G979 knockout heterozygous plants showed impaired (delayed) germination. Upon germination, these seeds produced extremely tiny seedlings that often did not survive transplantation. A few small and sickly looking homozygous plants could be grown, which produced siliques that contained seeds that were small and wrinkled compared to wild type.

A second, different, T-DNA insertion allele for G979 was identified as part of a TAIL PCR screen. Progeny of the heterozygous plant carrying that T-DNA insertion was either wild-type or heterozygous for the mutation, providing additional evidence for the disruption of G979 being the cause of the phenotypic alterations detected.

The initial analysis of the gene was performed using overexpressing lines. 35S::G979 transformants were generally smaller than wild type and developed spindly inflorescences that carried abnormal flowers with compromised fertility.

G979 expression was ubiquitous and not induced under any of the conditions tested.

Potential Applications

On the basis of the results obtained with G979 knockout mutant lines, it is possible that G979 or its equivalogs could be used to alter or modify seed germination, ripening and development properties and performance.

G987 (SEQ ID NO: 203)

Published Information

The genomic sequence of G987 is located on the Arabidopsis BAC clone T914 (gene T914.14) (GenBank accession number AC005315).

Experimental Observations

As determined by RT-PCR analysis, G987 was constitutively expressed in all tissues tested. A line homozygous for a T-DNA insertion in G987 was used to determine the function of this gene. The T-DNA insertion in G987 was approximately 4% into the coding sequence of the gene, and therefore is likely to result in a null mutation. G987 mutant plants could only be grown on sucrose-containing medium. Biochemical analyses of leaves from G987 mutants grown on sucrose-containing medium indicate that the mutants had reduced amounts of 16:3 fatty acids, the presence of two xanthophylls which were not present in wild-type leaves, the presence of gamma-tocopherol (which normally accumulates in seed tissue), and reduced levels of chlorophyll a and chlorophyll b.

Potential Applications

The low amount of 16:3 and dramatic reduction in chlorophyll indicated that the gene controls some aspect of thylakoid membrane development. G987 or its equivalogs may control proplastid to chloroplast development. This could be tested by measuring the expression of some of the genes (e.g. LHCP) that are associated with the transition from proplastid to chloroplast. If this were the case, the gene or its equivalogs may be useful for controlling the transition from proplastid to chromoplast in fruits and vegetables. There may also be some applications where it would be desirable to change the expression of the gene or its equivalogs (e.g., prevent cotyledon greening in Brassica napus or campestris to avoid green oil due to early frost).

G988 (SEQ ID NO: 205)

Published Information

G988 corresponds to a protein annotated as hypothetical in BAC F20N2 (GenBank accession number AC002328) from chromosome 1 of Arabidopsis. The sequence for G988 can also be found on the chromosome 1 BAC clone T5A14 and is described in patent application WO 98/46759.

Closely Related Genes from Other Species

The amino acid sequence for the Capsella rubella hypothetical protein represented by GenBank accession number CRU303349 was significantly identical to G988 outside of the SCR conserved domains. The Capsella rubella hypothetical protein is 90% identical to G988 over a stretch of roughly 450 amino acids. Therefore, it is likely that the Capsella rubella gene is an ortholog of G988.

Experimental Observations

G988 (SEQ ID NO ID NO: 205) was analyzed using transgenic plants in which G988 was expressed under the control of the 35S promoter. Plants overexpressing G988 had multiple morphological phenotypes. The transgenic plants were generally smaller than wild-type plants, had altered leaf, inflorescence and flower development, altered plant architecture, and altered vasculature.

Plants overexpressing G988 were found to have decreased seed oil and increased seed protein. In one transgenic line overexpressing G988, an increase in the seed glucosinolate M39489 was observed.

In wild-type plants, G988 was expressed primarily in flower and silique tissue, but was also present at detectable levels in all other tissues tested. Expression of G988 was induced in response to heat treatment, and repressed in response to infection with Erysiphe.

Potential Applications

Based on the observed morphological phenotypes of the transgenic plants, G988 or its equivalogs can be used to create plants with larger flowers. This can have value in the ornamental horticulture industry. The reduction in the formation of lateral branches suggests that G988 can have utility on the forestry industry. The Arabidopsis plants overexpressing G988 also had reduced fertility. This could actually be a desirable trait in some instances, as it can be exploited to prevent or minimize the escape of GMO (genetically modified organism) pollen into the environment.

G988 may also be used to modify seed oil and protein content.

G1040 (SEQ ID NO: 207)

Published Information

G1040 was identified in the sequence of BAC MFO20, GenBank accession number AB013391, released by the Arabidopsis Genome Initiative. G1040 has been published as KAN4, one of a clade of four KANADI genes that are thought to promote abaxial cell fates in lateral organs (Eshed et al. (2001) Current Biology 11: 1251-1260).

Experimental Observations

A full-length cDNA corresponding to G1040 was isolated. The function of this gene was analyzed using transgenic plants in which G1040 was expressed under the control of the 35S promoter. Plants overexpressing G1040 were found to produce seeds that were generally smaller and more rounded than control seeds, with a high proportion of irregularly-shaped seeds. No other morphological, physiological, or biochemical alterations were observed in these plants. G1040 may affect embryo development. G1040 was expressed throughout the plant, though at lower levels in shoots and rosette leaves than in other tissues.

Potential Applications

G1040 or its equivalogs could be used to manipulate seed size and shape.

G1047 (SEQ ID NO: 209)

Published Information

G1047 was identified in the sequence of BAC T20K9, GenBank accession number AC004786, released by the Arabidopsis Genome Initiative.

Experimental Observations

The boundaries of G1047 were experimentally determined and the function of G1047 was analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter. G1047 overexpressing lines were more tolerant to infection with a moderate dose of the fungal pathogen Fusarium oxysporum. G1047 overexpression did not seem to have consistent a detrimental effect on plant growth or vigor, and the lines tested for resistance were reported as being wild-type morphologically. In addition, no difference was detected between those lines and the corresponding wild-type controls in all the biochemical assays that were performed.

G1047 was ubiquitously expressed, and it was not significantly induced under any of the conditions tested

Potential Applications

G1047 transgenic plants have an altered response to the fungal pathogen Fusarium oxysporum. Therefore, G1047 or its equivalogs could be used to manipulate the defense response in order to generate pathogen-resistant plants.

G1051 (SEQ ID NO: 211)

Published Information

G1051 was initially identified in the sequence of BAC-end B77139 and subsequently the entire sequence of G1051 was disclosed in the sequence of BAC accession number AC005956, released by the Arabidopsis genome initiative.

Closely Related Genes from Other Species

G1051 is very similar to a rice bZIP transcription factor, accession number BAA96162, identified as part of the rice genome sequencing project. Homology between G1051 and this rice protein continues beyond the conserved domain, suggesting that they are orthologous.

Experimental Observations

The boundaries of G1051 were experimentally determined and the function of this gene was analyzed using transgenic plants in which G1051 was expressed under the control of the 35S promoter. Plants overexpressing G1051 exhibited a delay in flowering and typically produced flower buds about one week later than controls in continuous light conditions. G1051 was constitutively expressed throughout the plant and not induced by any condition tested.

Potential Applications

G1051 or its equivalogs could be used to manipulate flowering time in plants

G1052 (SEQ ID NO: 213)

Published Information

G1052 was identified in the sequence of BAC F9D24, GenBank accession number AL137081, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G1052 is similar to a rice gene BAA96162. Homology between G1052 and the rice gene extends beyond the conserved domain, thus the two genes may be orthologous.

Experimental Observations

The boundaries of G1052 in BAC AL137081 were experimentally determined and the function of G1052 was analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter. Plants overexpressing G1052 exhibited a delay in flowering and typically produced flower buds about one week later than controls in continuous light conditions. Additionally, these plants had larger leaves and were generally more sturdy than wild type.

A line homozygous for a T-DNA insertion in G1052 was also used to determine the function of this gene. The T-DNA insertion of G1052 was approximately one third of the way into the coding sequence of the gene and therefore is likely to result in a null mutation. A decrease in the percentage of lutein and increase in the xanthophyll 1 fraction was detected in one line in two experiments.

Potential Applications

The flowering time phenotype associated with G1052 over-expression indicates a utility for G1052 or its equivalogs as genes that can be used to manipulate flowering time in commercial plants. In addition, if the G1052 can not be transmitted through pollen, G1052 or its equivalogs may be used as a tool for preventing transgenes from escaping from transgenic plants through pollen dispersal.

G1052 or its equivalogs could be used to manipulate seed prenyl lipid composition. Lutein is an important nutraceutical, since lutein-rich diets have been shown to help prevent age-related macular degeneration (ARMD), which is the leading cause of blindness in people over the age of 65. In particular, consumption of dark green leafy vegetables has been shown in clinical studies to reduce the risk of ARMD. In addition, lutein, like other xanthophylls such as zeaxanthin and violaxanthin, is an essential component in the protection of the plant against the damaging effects of excessive light. Specifically, lutein contributes, directly or indirectly, to the rapid rise of nonphotochemical quenching in plants exposed to high light. Crop plants engineered to contain higher levels of lutein could therefore have improved photoprotection, possibly leading to less oxidative damage and better growth under high light.

G1062 (SEQ ID NO: 215)

Published Information

G1062 corresponds to gene MLJ15.14 (BAB01738.1).

Closely Related Genes from Other Species

G1062 protein shares extensive homology in the basic helix loop helix region with a cDNA from developing stem Medicago truncatula (AW691174) as well as a tomato shoot/meristem Lycopersicon esculentum cDNA (BG123327).

Experimental Observations

G1062 is a proprietary sequence initially identified from a library clone. The function of G1062 was analyzed by knockout analysis. The T-DNA insertion of G1062 was approximately 75% into the coding sequence of the gene and therefore is likely to result in a null mutation.

Homozygotes for a T-DNA insertion in G1062 showed slow growth and produced abnormal seeds. Knockout.G1062 plants displayed a longer leaf plastochron than wild type. Both generated flower buds at the same time, but wild-type plants had produced 9-11 rosette leaves at that point, compared to only 5-9 rosette leaves in the mutant (24 hour light). Following bolting, KO.G1062 inflorescences developed more slowly and were shorter than wild type. Knockout G1062 seeds appeared twisted and wrinkled in comparison to wild-type seed.

Physiological assays revealed that seedlings from a G1062 knockout mutant line have a light grown phenotype in the dark and were more severely stunted in an ethylene insensitivity assay when compared to the wild-type controls. This result indicated that G1062 may be involved in the ethylene triple response pathway. It is well known that ethylene is involved in the seed ripening process and therefore, the abnormal seed phenotype could be related to a general sensitivity to ethylene signal transduction pathway.

RT-PCR analysis indicated that the transcripts of G1062 were predominantly accumulated in the reproductive tissues. Its expression level appeared to be not affected by any treatments tested.

Potential Applications

G1062 or its equivalogs that alter seed shape are likely to provide ornamental applications.

Since G1062 is involved in the ethylene triple response pathway, G1062 could be used to manipulate seed or fruit ripening process, and to improve seed or fruit quality.

G1063 (SEQ ID NO: 217)

Published Information

G1063 corresponds to gene K21H1.2 (BAB10940.1).

Closely Related Genes from Other Species

G1063 protein shared extensive homology in the basic helix loop helix region with a protein sequence encoded by Glycine max cDNA clone (AW832545) as well as a tomato root, plants pre-anthesis Lycopersicon sculentum cDNA (BE451174).

Experimental Observations

G1063 (SEQ ID NO: 217) is a member of a clade of highly related HLH/MYC proteins that also includes G779, G1499, G2143, and G2557. All of these genes caused similar pleiotropic phenotypic effects when overexpressed, the most striking of which was the production of ectopic carpelloid tissue. These genes can be considered key regulators of carpel development. A spectrum of developmental alterations was observed amongst 35S::G1063 primary transformants and the majority were markedly small, dark green, and had narrow curled leaves. The most severely affected individuals were completely sterile and formed highly abnormal inflorescences; shoots often terminated in pin-like structures, and flowers were replaced by filamentous carpelloid structures. In other cases, flowers showed internode elongation between floral whorls, with a central carpel protruding on a pedicel-like organ. Additionally, lateral branches sometimes failed to develop and tiny patches of carpelloid tissue formed at axillary nodes of the inflorescence. In lines with an intermediate phenotype, flowers contained defined whorls of organs, but sepals were converted to carpelloid structures or displayed patches of carpelloid tissue. In contrast, lines with a weak phenotype developed relatively normal flowers and produced a reasonable quantity of seed. Such plants were still distinctly smaller than wild-type controls. Since the strongest 35S::G1063 lines were sterile, three lines with a relatively weak phenotype, that had produced sufficient seed for biochemical and physiological analysis, were selected for further study. Two of the T2 populations (T2-28,37) were clearly small, darker green and possessed narrow leaves compared to wild type. Plants from one of these populations (T2-28) also produced occasional branches with abnormal flowers like those seen in the T1. The final T2 population (T2-30) displayed a very mild phenotype. Overexpression of G1063 in Arabidopsis resulted in a decrease in seed oil content in two T2 lines. No altered phenotypes were detected in any of the physiological assays, except that the plants were noted to be somewhat small and produce anthocyanin when grown in Petri plates. G1063 was expressed at low to moderate levels in roots, flowers, rosette leaves, embryos, and germinating seeds, but was not detected in shoots or siliques. G1063 was induced by auxin

Potential Applications

G1063 or its equivalogs can be used to manipulate flower form and structure or plant fertility. One application for manipulation of flower structure can be in the production of saffron, which is derived from the stigmas of Crocus sativus. G1063 has utility in manipulating seed oil and protein content.

G1064 (SEQ ID NO: 219)

Closely Related Genes from Other Species G1064 protein shares a close homology to an auxin-induced basic helix-loop-helix transcription factor from Gossypium hirsutum (PID:5731257) in the bHLH motif region as well as outside of this region. G1064 also has high similarity to a tomato germinating seedlings cDNA clone (AW649873). Experimental Observations

G1064 was initially identified from a library clone collection. The function of G1064 was analyzed using transgenic plants in which G1064 was expressed under the control of the 35S promoter.

Physiological assays revealed that G1064 overexpressing lines were more susceptible to infection with a low dose of the fungal pathogen Botrytis cinerea compared to the wild-type controls.

No morphological and biochemical alterations were observed in the overexpressing transgenic plants when compared to wild-type controls. Furthermore, RT-PCR analyses of the endogenous levels of G1064 indicated that this gene was uniformly expressed in all tissues and under all conditions tested.

Potential Applications

Since G1064 transgenic plants have an altered response to the pathogen Botrytis cinerea, G1064 or its equivalogs could be used to manipulate the defense response in order to generate pathogen-resistant plants.

G1069 (SEQ ID NO: 221)

Published Information

The sequence of G1069 was obtained from EU Arabidopsis sequencing project, GenBank accession number Z97336, based on its sequence similarity within the conserved domain to other AT-Hook related proteins in Arabidopsis.

Closely Related Genes from Other Species

G1069 protein shares a significant homology to a cDNA isolated from Lotus japonicus nodule library. Similarity between G1069 and the Lotus cDNA extends beyond the signature motif of the family to a level that would suggest the genes are orthologous. Therefore the gene represented by EST AW720668 may have a function and/or utility similar to that of G1069.

Experimental Observations

The sequence of G1069 was experimentally determined and the function of G1069 was analyzed using transgenic plants in which G1069 was expressed under the control of the 35S promoter.

Plants overexpressing G1069 showed changes in leaf architecture, reduced overall plant size, and retarded progression through the life cycle. This is a common phenomenon for most transgenic plants in which AT-HOOK proteins are overexpressed if the gene is predominantly expressed in root in the wild-type background. G1069 was predominantly expressed in roots, based on analysis of RT-PCR results. To minimize these detrimental effects, G1069 may be overexpressed under a tissue specific promoter such as root- or leaf-specific promoter or under inducible promoter.

One of G1069 overexpressing lines showed more tolerance to osmotic stress when they were germinated in high sucrose plates. This line also showed insensitivity to ABA in a germination assay.

Potential Applications

The osmotic stress results indicate that G1069 could be used to alter a plant's response to water deficit conditions and, therefore, the gene or its equivalogs could be used to engineer plants with enhanced tolerance to drought, salt stress, and freezing.

G1069 affects ABA sensitivity, and thus when transformed into a plant the gene or its equivalogs may diminish cold, drought, oxidative and other stress sensitivities, and also be used to alter plant architecture, and yield.

G1073 (SEQ ID NO: 223)

Published Information

G1073 has been identified in the sequence of a BAC clone from chromosome 4 (BAC clone F23E12, gene F23E12.50, GenBank accession number AL022604), released by EU Arabidopsis Sequencing Project.

Closely Related Genes from Other Species

G1073 has similarity to Medicago truncatula cDNA clones (GenBank accession number AW574000 and AW560824) and Glycine max cDNA clones (AW349284 and AI736668) in the database.

Experimental Observations

The function of G1073 was analyzed using transgenic plants in which G1073 was expressed under the control of the 35S promoter. Transgenic plants overexpressing G1073 were substantially larger than wild-type controls, with at least a 60% increase in biomass. The increased mass of 35S::G1073 transgenic plants was attributed to enlargement of multiple organ types including leaves, stems, roots and floral organs. Petal size in the 35S::G1073 lines was increased by 40-50% compared to wild type controls. Petal epidermal cells in those same lines were approximately 25-30% larger than those of the control plants. Furthermore, 15-20% more epidermal cells per petal were produced compared to wild type. Thus, at least in petals, the increase in size was associated with an increase in cell size as well as in cell number. Additionally, images from the stem cross-sections of 35S::G1073 plants revealed that cortical cells are large and that vascular bundles contained more cells in the phloem and xylem relative to wild type

Seed yield was increased compared to control plants. 5S::G1073 lines showed an increase of at least 70% in seed yield. This increased seed production was associated with an increased number of siliques per plant, rather than seeds per silique.

Flowering of G1073 overexpressing plants was delayed. Leaves of G1073 overexpressing plants were generally more serrated than those of wild-type plants. Improved drought tolerance was observed in 35S::G1073 transgenic lines.

Potential Applications

Transgenic plants overexpressing G1073 are large and late flowering with serrated leaves. Large size and late flowering produced as a result of G1073 or equivalog overexpression would be extremely useful in crops where the vegetative portion of the plant is the marketable portion (often vegetative growth stops when plants make the transition to flowering). In this case, it would be advantageous to prevent or delay flowering with the use of this gene or its equivalogs in order to increase yield (biomass). Prevention of flowering by this gene or its equivalogs would be useful in these same crops in order to prevent the spread of transgenic pollen and/or to prevent seed set. This gene or its equivalogs could also be used to manipulate leaf shape and drought tolerance.

G1075 (SEQ ID NO: 225)

Published Information

The sequence of G1075 was obtained from the Arabidopsis genome sequencing project, GenBank accession number AC004667, based on its sequence similarity within the conserved domain to other AT-Hook related proteins in Arabidopsis.

Closely Related Genes from Other Species

G1075 is homologous to a Medicago truncatula cDNA clone (acc#AW574000

Experimental Observations

The function of G1075 was analyzed using transgenic plants in which G1075 was expressed under the control of the 35S promoter. Overexpression of G1075 produced very small, sterile plants. Pointed leaves were noted in some seedlings, and twisted or curled leaves and abnormal leaf serrations were noted in rosette stage plants. Bolts were short and thin with short internodes. Flowers from severely affected plants had reduced or absent petals and stamen filaments that partially or completely fail to elongate. Because of the severe phenotypes of these T1 plants, no T2 seed was produced for physiological and biochemical analysis.

RT-PCR analysis indicated that G1075 transcripts are found primarily in roots. The expression of G1075 appeared to be induced by cold and heat stresses.

Potential Applications

G1075 or its equivalogs could be used to modify plant architecture and development, including flower structure. If expressed under a flower-specific promoter, the gene or its equivalogs might also be useful for engineering male sterility. Because expression of G1075 is root specific, its promoter could be useful for targeted gene expression in this tissue.

G1084 (SEQ ID NO: 227)

Published Information

G1084 was discovered as a type 2 bZIP gene in BAC F19F24, accession number AC002392, released by the Arabidopsis genome initiative.

Experimental Observations

The boundaries of G1084 were experimentally determined and the function of G1084 was analyzed using transgenic plants in which G1084 was expressed under the control of the 35S promoter.

Plants overexpressing G1084 showed more disease symptoms following inoculation with a low dose of the fungal pathogen Botrytis cinerea. G1084 expression appeared to be restricted to flowers and embryos and was not significantly induced by any conditions tested. Interestingly, one of twenty T1 plants showed heritable alterations in flower development. One explanation for this is that this phenotype was caused by silencing of endogenous G1084 in that particular line, causing a phenotype similar to that produced if G1084 was knocked out. No altered phenotypes were detected in any biochemical assay performed.

Potential Applications

G1084 or its equivalogs could be used to manipulate the plant defense response to produce pathogen-resistant plants.

G1089 (SEQ ID NO: 229)

Published Information

G1089 was initially identified as a gene represented by Arabidopsis EST H37430. Subsequently, the entire sequence of G1089 was identified in BAC F19K6, GenBank accession number AC037424, released by the Arabidopsis genome initiative.

Closely Related Genes from Other Species

The most related gene to G1089 is a rice gene represented by NCBI entry g13124871. Similarity between G1089 and the rice gene extends beyond the signature motif of the family to a level that would suggest the genes are orthologous. Therefore the gene represented by the rice gene may have a function and/or utility similar to that of G1089

Experimental Observations

The boundaries of G1089 were experimentally determined and the function of G1089 was analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter. G1089 overexpressing plants had reduced seedling vigor and were characterized as being small, yellow and sickly looking. In addition, a T-DNA knockout of G1089 was isolated. G1089 knockout mutant plants showed more tolerance to osmotic stress in a germination assay in two separate experiments. They showed more seedling vigor than wild-type control when germinated on plates containing high sucrose. G1089 appeared to be constitutively expressed.

Potential Applications

The osmotic stress results indicate that G1089 or its equivalogs could be used to alter a plant's response to water deficit conditions and, therefore, may be used to engineer plants with enhanced tolerance to drought, salt stress, and freezing.

G1134 (SEQ ID NO: 231)

Published Information

A partial sequence of G1134 was identified from an EST clone (GenBank accession number AI099951).

Experimental Observations

A partial sequence of G1134 was identified from an EST clone (GenBank accession number AI099951). The 5′ end of the G1134 coding sequence was determined by RACE. The function of G1134 was analyzed using transgenic plants in which G1134 was expressed under the control of the 35S promoter. Primary transformants of G1134 were small with strongly curled leaves. In the T2 generation, two lines had narrow, somewhat curled leaves and siliques with altered shape. A third line segregated for small size. Additionally, plants overexpressing G1134 showed an altered response to the growth hormone ethylene. Seeds that were germinated on ACC plates in the dark had longer hypocotyls than the corresponding controls and occasionally lacked the apical hook that is part of a typical ethylene triple response. In addition, seeds from all lines germinated in the dark have a partial light grown phenotype in that their cotyledons are open and the hypocotyl is straight instead of curled.

The results from morphological and physiological analysis indicated that G1134 protein may play important roles in the regulation of ethylene biosynthesis, ethylene signal transduction pathways, or photomorphogenesis. Analysis of G1134 overexpressors revealed no apparent biochemical changes when compared to wild-type control plants. Analysis of the endogenous expression level of G1134, as determined by RT-PCR, revealed that G1134 was predominantly expressed in flower tissues. Expression of G1134 was not induced by any of the environmental conditions or pathogens tested.

Potential Applications

G1134 or its equivalogs could be used to alter how plants respond to ethylene and/or light. For example, it could be used to manipulate fruit ripening.

G1140 (SEQ ID NO: 233)

Published Information

G1140 corresponds to gene AT4g24540 (CAB79364), and has also been referred to as AGL24 (Alvarez-Buylla et al. (2000a) Proc. Natl. Acad. Sci. USA 97:5328-5333; Alvarez-Buylla et al. (2000b) Plant J. 2000 24:457-466).

Closely Related Genes from Other Species

G1140 shows sequence similarity outside of the conserved MADS domain with a variety of MADS proteins from different plant species, such as gi13448660 (MADS box transcription factor from Ipomoea batatas).

Experimental Observations

The function of G1140 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter.

Overexpression of G1140 produced marked alterations in flower morphology, which were initially observed in a relatively small proportion (6/20 lines) of the T1 plants. Alterations included variations in organ size and number. In particular, increased numbers of petals and sepals were often present, and sometimes small carpel-like outgrowths were fused to the central pair of carpels. Additionally, petals sometimes displayed leaf-like characteristics. It should be noted, however, that these abnormalities were most prevalent in early flowers and that later-arising flowers were sometimes normal. Two of the lines selected for subsequent studies showed these floral phenotypes, which became much more extreme in the T2 populations. Some of the effects resembled those produced by strong apetala1 or apetala2 alleles. Lowermost floral nodes were replaced by shoot-like structures, which became increasingly flower-like towards the top of the inflorescence. In the lower structures, floral organs were not apparent and were replaced by bract-like organs that were not organized into whorls. Later ‘flowers’ had sepals and petals replaced by bract like organs, but individual ‘whorls’ could be discerned. Stamens and carpels often had bract-like characteristics and carpels were frequently unfused. Furthermore, internode elongation was commonly observed between floral whorls.

G1140 is a MADS box gene of the MIKC-type, and many members of that subfamily are involved in the control of flower development. Determination of the functions of MADS box genes has often required the characterization of loss-of-function mutants. However, G1140 knockout mutant plants were wild-type in morphology and development, as well as in the physiological and biochemical analyses that were performed. In that mutant line, the T-DNA insertion lies shortly downstream of the conserved MADS domain, within an exon. Within the Arabidopsis MADS-box gene family, G1140 is most closely related to G861/SHORT VEGETATIVE PHASE, which is involved in the floral transition (Hartmann et al. (2000) Plant J. 21:351-360).

G1140 was expressed in roots, leaves, shoots, and floral tissues. G1140 expression was not detected in embryo, siliques, or germinating seedlings. The expression of the gene did not appear to be significantly induced by any of the conditions tested.

Potential Applications

Based on the phenotypes observed in 35S::G1140 plants, the gene or its equivalogs could be used to manipulate flower structure and development.

G1143 (SEQ ID NO: 235)

Published Information

The sequence of G1143 was obtained from the Arabidopsis genome sequencing project, GenBank accession number AL031187, based on its sequence similarity within the conserved domain to other bHLH/Myc related proteins.

Experimental Observations

The function of G1143 was analyzed using transgenic plants in which G1143 was expressed under the control of the 35S promoter. 35S::G143 transgenic plants showed no consistent differences in morphology to wild-type controls. In a first sowing of the T2 populations, it was observed that the plants were possibly early flowering. However, this phenotype was not apparent in either a replant of the T2 lines or in any T1 plants.

As measured by NIR, G1143 overexpressing plants were found to have decreased seed oil content and increased seed protein content compared to wild-type plants.

Potential Applications

G1143 or equivalog overexpression may be used to alter seed oil and seed protein content in plants, which may be very important for the nutritional value and production of various food products.

G1146 (SEQ ID NO: 237)

Published Information

G1146 corresponds to the ZWILLE and PINHEAD/ZWILLE gene described by Moussain et al. ((1998) EMBO J. 17: 1799-1809) and Lynn et al. ((1999) Development 126: 469-481). Moussain et al. have shown that G1146 is required to establish the central-peripheral organization of the embryo apex and that this step is critical for shoot meristem self-perpetuation. They indicate that G1146 is required to maintain stem cells of the developing shoot meristem in an undifferentiated state during the transition from embryonic development to repetitive post-embryonic organ formation. Based upon the results of Moussain et al from in situ hybridization analysis, G1146 is found in provascular cells at all stages of development.

Lynn et al. describe the phenotype of a plant with a mutation on G1146. Early in development, G146 mutant plants have abnormal embryos, with aberrant division of the upper cells of the suspensor. In young seedling, there is a radially symmetric pin-like structure in the position normally occupied by the shoot apical meristem. As development proceeds, new shoot meristems eventually arise in the axils of the cotyledons. Phenotypes observed in older plants include trumpet-shaped leaves and abnormalities in the primary inflorescence. Based upon their results from northern blot analysis, G1146 expression can be detected in roots, leaves, siliques and inflorescences of developing and mature plants. In the developing embryo, G1146 expression is found in the embryo proper and in the uppermost cell of the suspensor, as determined by in situ hybridization analysis.

Closely Related Genes from Other Species

The amino acid sequence for a region of the Oryza sativa chromosome 6 clone OJ1057_A09 (GenBank accession number AP003986) is significantly identical to G1146 outside of the PAZ conserved domains. Therefore, the gene represented by this region of the rice clone may be the ortholog of G1146.

Experimental Observations

The function of this gene was analyzed using transgenic plants in which G1146 was expressed under the control of the 35S promoter. Transgenic plants overexpressing G1146 had leaves that had a severe inward curl. The phenotype of these transgenic plants was wild-type in all other assays performed. G1146 expression was detected in all tissues tested, with expression being highest in flowers, rosette tissue, developing seeds and siliques. Expression of G1146 was not induced by any of the environmental or stress conditions tested.

Potential Applications

On the basis of analyses performed to date, G1146 or its equivalogs can be used in ornamental horticulture to create plants with altered leaf morphology.

G1196 (SEQ ID NO: 239)

Published Information

G1196 was identified by amino acid sequence similarity to ankyrin repeat proteins. G1196 is found in the sequence of the 4, BAC clone T16H5 (GenBank AL024486.1 GI:3250673), released by the Arabidopsis Genome Initiative. The start and stop codons were correctly predicted. The closest homologous Arabidopsis protein is NPR1, which is required for development of systemic acquired resistance in plants (Cao et al. (1997) Cell 88:57-63).

Experimental Observations

RT-PCR analysis of the endogenous level of G196 transcripts revealed low constitutive expression in all tissue examined. G1196 transcript levels increased upon auxin, ABA, cold, heat and salt treatment, as well as 7 days post-inoculation with Erysiphe orontii. Plants treated with SA showed moderate accumulation of G1196 transcripts. The physiological analysis of a G1196 null mutant line revealed increased susceptibility to a low dose inoculum of Botrytis cinerea. This finding indicated that G1196 may play a similar role to NPR1 in disease pathways. Apart from disease susceptibility, the functional characterization of the G196 null mutant revealed no significant changes in the biochemical profile, the morphology and development, or the response to biotic/abiotic stress treatments in comparison to the wild-type controls.

Potential Applications

Lack of G196 activity in a null mutant has been shown to affect the onset of disease following inoculation with Botrytis cinerea. Therefore, G1196 or its equivalogs could be used to manipulate the defense response in order to generate pathogen-resistant plants.

G1198 (SEQ ID NO: 241)

Published Information

The entire sequence of G1198 was reported in BAC T23G18, accession number AC011438, released by the Arabidopsis genome initiative.

Closely Related Genes from Other Species

G198 is very similar to the tobacco bZIP transcription factor TGA2.2 (accession number AF031487). Similarity extends well beyond the conserved domain, suggesting that G1198 and TGA2.2 have similar functions.

Experimental Observations

The boundaries of G1198 were experimentally determined and the function of G1198 was analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter. G1198 overexpressing plants were reduced in size with smaller, narrower leaves and had significantly increased levels of a glucosinolate as compared to wild type. G1198 did not appear to be expressed in rosette leaves, but was expressed in other tissues.

G1198 overexpressing plants were found to have increased seed oil content, as compared to wild-type plants.

Potential Applications

G198 or equivalog overexpression maybe used to alter seed oil content in plants, which may be very important for the nutritional value and production of various food products.

G1225 (SEQ ID NO: 243)

Published Information

The sequence of G1225 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AB016882, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis.

Experimental Observations

The complete sequence of G1225 was determined. G1225 expression was detected in rosette leaves, flowers, embryos and siliques. No expression was detected in shoots, roots or germinating seeds. G1225 was not induced by any condition tested. It may possibly be repressed by cold and Erysiphe infection.

The function of this gene was analyzed using transgenic plants in which G1225 was expressed under the control of the 35S promoter. G1225 overexpressors showed greener cotyledons and longer roots on high sucrose and glucose containing media compared to wild-type controls. This effect was seen in two of the three lines tested. G1225 may thus be involved in sugar sensing. Plants overexpressing G1225 were also found to flower earlier than control plants. 35S::G1225 transformants from two independent T2 lines produced visible flower buds several days earlier than controls, in each of two separate plantings. A similar decrease in flowering time was also seen in thirteen out of twenty T1 lines. In fact, 35S::G1225 seedlings appeared to develop rather more rapidly than wild type and progressed through the lifecycle at a faster rate. Overexpression of G1225 in Arabidopsis did not result in any biochemical phenotypic alteration.

The sugar sensing phenotype of G1225 overexpressing plants may be related to the early flowering phenotype. Sugars are central regulatory molecules that control several aspects of plant physiology, metabolism, and development, including flowering.

Potential Applications

G1225 or its equivalogs may be useful for accelerating flowering time.

The sugar sensing phenotype of G1225 indicates that this gene or its equivalogs may be also useful for altering source-sink relationships or other sugar regulated processes.

G1226 (SEQ ID NO: 245)

Experimental Observations

The function of this gene was studied using transgenic plants in which G1226 was expressed under the control of the 35S promoter. Approximately 50% of 35S::G1226 primary transformants flowered earlier than wild-type controls under continuous light conditions. However, no correlation was noted between transgene expression level (determined by RT-PCR, not shown) and this phenotype; some T1 plants that appeared wild-type clearly expressed the transgene. Marginally early flowering was noted in one of three T2 lines, but the other two lines appeared wild-type. Kanamycin segregation data indicated that all three lines contained single locus transgene insertions. RT-PCR analysis indicates that G1226 was constitutively expressed in all tissues, with the exception of roots.

As measured by NIR, G1226 overexpressors had increased seed oil content compared to wild-type plants.

Potential Applications

G1226 or equivalog overexpression may be used to alter seed oil content, which may be very important for the nutritional value and production of various food products

G1226 or its equivalogs could be used to manipulate the flowering time.

G1229 (SEQ ID NO: 247)

Experimental Observations

RT-PCR analysis indicated that G1229 was expressed in all tissues except roots. Its expression level was increased by auxin treatment and repressed by Erysiphe treatment.

The function of G1229 was studied using transgenic plants in which this gene was expressed under the control of the 35S promoter. Overexpression of G1229 strongly influenced plant development. G1229 T1 overexpressing plants were consistently small, paler in color, had rounder leaves, and were slower growing than wild type. These effects were attenuated to some extent in the T2 generation, but were still apparent in two of the three lines analyzed. Physiological assays revealed that G1229 overexpressing lines had reduced seed germination and seedling vigor compared to wild-type plants when grown on MS plates. Plants from G1229 overexpressing lines also showed an ethylene sensitive phenotype when germinated in the dark on media containing ACC. However, because germination was generally poor, the interpretation of this phenotype is difficult.

A single line showed a number of additional phenotypic differences; in this line flower structure was altered and abnormal seed was produced that appeared darker and more wrinkled than wild-type seed. Seeds from this line showed a significant decrease in oil content as measured by NIR. This observation has been repeated. It is possible that these multiple phenotypes could be due to disruption of an endogenous gene by the transgene insertion, rather than to overexpression of G1229.

Potential Applications

Based on the current analysis of G1229 overexpressing plants, potential utilities for G1229 or its equivalogs are decrease seed oil contents in crop plants.

G1255 (SEQ ID NO: 249)

Published Information

G1255 was identified as a gene in the sequence of BAC AC079281, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G1255 showed strong homology to a putative rice zing finger protein represented by sequence AC087181_(—)3. Sequence identity between these two proteins extends beyond the conserved domain, and therefore, these genes can be orthologs.

Experimental Observations

The sequence of G1255 (SEQ ID NO: 249) was experimentally determined and G1255 was analyzed using transgenic plants in which G1255 was expressed under the control of the 35S promoter. Plants overexpressing G1255 had alterations in leaf architecture, a reduction in apical dominance, an increase in seed size, and showed more disease symptoms following inoculation with a low dose of the fungal pathogen Botrytis cinerea. G1255 was constitutively expressed and not significantly induced by any conditions tested

Potential Applications

On the basis of the phenotypes produced by overexpression of G1255, G1255 or its equivalogs can be used to manipulate the plant's defense response to produce pathogen resistance, alter plant architecture, or alter seed size.

G1266 (SEQ ID NO: 251)

Published Information

G1266 corresponds to ERF1, ‘ethylene response factor 1’ (GenBank accession number AF076277) (Solano et al. (1998) Genes Dev. 12: 3703-3714). ERF1 was isolated in a search for Arabidopsis EREBP-like genes using a PCR-based approach. ERF1 expression was shown to be rapidly induced by ethylene, and to be dependent on the presence of functional EIN3 (ETHYLENE-INSENSITIVE3), as no expression was detected in ein3-1 mutants (Solano et al. (1998) supra). Furthermore, ERF1 mRNA showed constitutive high-level expression in 35S::EIN3-expressing transgenic plants, and EIN3 was shown to bind to sequences in the ERF1 promoter in a sequence-specific manner (Solano et al. (1998) supra). All these results indicated that ERF1 is downstream of EIN3 in the ethylene signaling pathway, and that both proteins act sequentially in a cascade of transcriptional regulation initiated by ethylene gas (Solano et al. (1998) supra). ERF1 binds specifically to the GCC element, which is a particular type of ethylene response element that is found in the promoters of genes induced upon pathogen attack (Solano et al., (1998) supra). 35S::ERF1-expressing transgenic plants displayed phenotypes similar to those observed in the constitutive ethylene response mutant ctrl or in wild-type plants exposed to ethylene; however, expression of only a partial seedling triple response in these lines indicated that ERF1 mediates only a subset of the ethylene responses (Solano et al. (1998) supra). At the adult stage, 35S::ERF1-expressing transgenic plants showed a dwarf phenotype, and some ethylene-inducible genes, like basic-chitinase and PDF1.2 were constitutively activated in those lines (Solano et al. (1998) supra). All these results showed that ERF1 is a downstream ethylene signaling pathway gene.

Closely Related Genes from Other Species

The sequences of Nicotiana tabacum S25-XP1 (GenBank accession number AAB38748) and G1266 are very similar, with similarity between the two proteins extending beyond the conserved AP2 domain.

Experimental Observations

The function of G1266 was further analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter. As expected from the previously published work, G1266 overexpressing plants showed a dwarf phenotype. In physiological assays, it was shown that G1266 overexpressing plants were more tolerant to infection with a moderate dose of the fungal pathogen Erysiphe orontii. The resistance phenotype to the fungal pathogen Erysiphe orontii has been repeated. This phenotype might be a consequence of ERF1 being a downstream ethylene signaling pathway gene. Constitutive expression of G1266 might accelerate leaf senescence, which in turn might impair infection by Erysiphe orontii.

In addition, when analyzed for leaf insoluble sugar composition, three lines showed alterations in rhamnose, arabinose, xylose, and mannose, and galactose when compared with wild-type plants.

Potential Applications

G1266 has been shown to be a downstream ethylene signaling pathway gene, and experiments implicate this gene in the plant response to the fungal pathogen Erysiphe orontii. G1266 or its equivalogs could therefore be used to engineer plants with a modulated response to that and other pathogens, for example, plants showing increased resistance.

G1275 (SEQ ID NO: 253)

Published Information

G1275 was first identified in the sequence of BAC T19G15 (GenBank accession number AC005965).

Experimental Observations

The cDNA sequence of G1275 was determined. G1275 was ubiquitously expressed, although expression levels differed among tissues. It is possible that G1275 expression is induced by several stimuli, including infection by Erysiphe, Fusarium, and SA treatment.

The function(s) of G1275 were investigated using both knock-out mutants and overexpressing plants in which this gene was expressed under the control of the 35S promoter.

Primary transformants of G1275 were small with reduced apical dominance. The inflorescence stems produced by these plants did not elongate normally. The plants were fertile, but seed yield was reduced because the plants were severely dwarfed.

In the knock-out mutant, the T-DNA insertion in G1275 was localized in the second intron of the gene, which is located within the conserved WRKY-box. Such insertion would result in a null mutation (unless the large fragment of exogenous sequence is perfectly spliced out from the transcribed G1275 pre-mRNA). G1275 knock-out mutant plants were indistinguishable from wild-type controls in all assays performed.

Potential Applications

G1275 or its equivalogs might be used to alter plant development or architecture.

G1305 (SEQ ID NO: 255)

Published Information

G1305 is a member of the (R1)R2R3 subfamily of myb transcription factors. G1305 corresponds to the gene MYB10 (Kranz et al. (1998) Plant J. 16: 263-276).

Experimental Observations

The function of G1305 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1305 in Arabidopsis resulted in seedlings that were more tolerant to heat in a germination assay. Seedlings from G1305 overexpressing transgenics were greener than the control seedlings under high temperature conditions. In a repeat experiment, two lines showed the heat tolerant phenotype. In addition, plants from two of the 35S::G1305 T2 lines flowered several days earlier than wild type in each of two independent sowings (24 hour light conditions). The plants had rather flat leaves compared to controls and formed slightly thin inflorescences in some cases.

According to RT-PCR, G1305 was expressed ubiquitously and expression of the gene was unaltered in response to the environmental stress-related conditions tested.

Potential Applications

On the basis of the analyses performed to date, the potential utility of G1305 or its equivalogs is to regulate a plant's time to flower.

G1305 or its equivalogs may also be used to improve heat tolerance at germination. The germination of many crops is very sensitive to temperature. A gene that would enhance germination in hot conditions may be useful for crops that are planted late in the season or in hot climates.

G1322 (SEQ ID NO: 257)

Published Information

G1322 is a member of the (R1)R2R3 subfamily of myb transcription factors. G1322 corresponds to Myb57, a gene identified by Kranz et al. ((1998) Plant J. 16: 263-276). The authors used a reverse-Northern blot technique to study the expression of this gene in a variety of tissues and under a variety of environmental conditions. They were unable to detect the expression of G1322 in any tissue or treatments tested (Kranz et al. (1998) Plant J. 16: 263-276).

Closely Related Genes from Other Species

G1322 shows sequence similarity with known genes from other plant species within the conserved Myb domain.

Experimental Observations

G1322 (SEQ ID NO: 257) was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G1322 transgenic plants were wild-type in phenotype with respect to the biochemical analyses performed. Overexpression of G1322 produced changes in overall plant size and leaf development. At all stages, 35S::G1322 plants were distinctly smaller than controls and developed curled dark-green leaves. Following the switch to flowering, the plants formed relatively thin inflorescence stems and had a rather poor seed yield. In addition, overexpression of G1322 resulted in plants with an altered etiolation response as well as enhanced tolerance to germination under chilling conditions. When germinated in the dark, G1322 overexpressing transgenic plant lines had open, slightly green cotyledons. Under chilling conditions, all three transgenic lines displayed a similar germination response, seedlings were slightly larger and had longer roots. In addition, an increase in the leaf glucosinolate M39480 was observed in all three T2 lines. According to RT-PCR analysis, G1322 was expressed primarily in flower tissue.

Potential Applications

The utilities of G1322 or its equivalogs include altering a plant's chilling sensitivity and altering a plant's light response. The germination of many crops is very sensitive to cold temperatures. A gene that will enhance germination and seedling vigor in the cold has tremendous utility in allowing seeds to be planted earlier in the season with a higher survival rate.

G1322 or its equivalogs can also be useful for altering leaf glucosinolate composition. Increases or decreases in specific glucosinolates or total glucosinolate content are desirable depending upon the particular application. Modification of glucosinolate composition or quantity can therefore afford increased protection from predators. Furthermore, in edible crops, tissue specific promoters can be used to ensure that these compounds accumulate specifically in tissues, such as the epidermis, which are not taken for consumption.

G1323 (SEQ ID NO: 259)

Published Information

Kranz et al. ((1998) Plant J. 16: 263-276) published a partial cDNA sequence corresponding to G1323, naming it MYB58. Reverse-Northern data indicates that this gene is expressed primarily in leaf tissue.

Experimental Observations

The complete sequence of G1323 was determined. As determined by RT-PCR, G1323 was highly expressed in embryos, and was expressed at significantly lower levels in the other tissues tested. G1323 expression was not induced by any stress-related treatments. The function of this gene was analyzed using transgenic plants in which G1323 was expressed under the control of the 35S promoter. Primary transformants of G1323 were uniformly small and dark green, and a few were late flowering. According to the biochemical analysis of G1323 overexpressors, two had higher seed protein. The higher seed protein and lower seed oil content was observed in a repeated experiment.

Potential Applications

G1323 or its equivalogs could be used to alter seed protein and oil amounts and/or composition, which is very important for the nutritional value and production of various food products.

G1330 (SEQ ID NO: 261)

Published Information

G1330 is a member of the R2-R3 subfamily of Myb transcription factors. Kranz et al. ((1998) Plant J. 16: 263-276) published a partial cDNA sequence corresponding to G1330, naming it MYB78. Expression of this gene was not detected by Reverse-Northern analysis in any tissue or under any environmental treatment tested.

Closely Related Genes from Other Species

G1330 is closely related to a family of novel myb-related genes (Cpm5, 7 and 10) from the resurrection plant Craterostigma plantagineum are specifically expressed in callus and roots in response to ABA or desiccation (Iturriaga et al. (1996) Plant Mol. Biol. 32: 707-716) as well as to myb genes from several other crop species. The most related gene to G1330 is a tomato gene represented by EST EST276215. Similarity between G1330 and the tomato gene extends beyond the signature motif of the family to a level that would suggest the genes are orthologous. Therefore the gene represented by EST 276215 and the cpm genes may have a function and/or utility similar to that of G1330.

Experimental Observations

The complete sequence of G1330 was determined. The function of this gene was analyzed using transgenic plants in which G1330 was expressed under the control of the 35S promoter. Overexpression of G1330 produced changes in plant growth and development. 35S::G1330 primary transformants were consistently small with abnormal phyllotaxy, and often developed spindly inflorescences that yielded few seeds. These effects were also observed in the T2 generation; all three lines appeared markedly small at the seedling stage and often did not survive the transfer from agar plates to soil. High anthocyanin levels were also noted in the T2 (and T3) seedlings of one line. At later stages, T2 plants appeared spindly and had very poor seed yield. Kanamycin segregation data were consistent with 35S::G1330 having deleterious effects; all three lines had a deficit of resistant plants, indicating that the transgene might be lethal above a certain threshold dosage.

Plants from G1330 overexpressing lines showed an ethylene insensitive phenotype when germinated in the dark on media containing ACC. Seedlings from the three lines tested lacked components of the triple response including the apical hook and to some degree, stunting of the hypocotyl. In addition, plants from the three overexpressing lines had open cotyledons in the dark, which indicated this gene is involved in a light dependent response,

As determined by RT-PCR, G1330 was highly expressed in roots, and was expressed at significantly lower levels in flowers, embryos and seedlings. No expression of G1330 was detected shoots, rosette leaves or siliques. G1330 expression was repressed in rosette leaves by cold, and osmotic stress treatments and by infection with the phytopathogen Erysiphe orontii.

Potential Applications

Because anti-oxidants such as tocopherols and carotenoids are reported to have anti-cancer and other nutritional properties, G1330 or its equivalogs could be used to manipulate the nutritional qualities of plants.

G1330 or its equivalogs could be used to alter how plants respond to ethylene. For example, it could be used to manipulate fruit ripening.

G1331 (SEQ ID NO: 263)

Published Information

G1331 is a member of the (R1)R2R3 subfamily of myb transcription factors. G1331 corresponds to Myb79, a gene identified by Kranz et al. ((1998) Plant J. 16: 263-276). The authors used a reverse-Northern blot technique to study the expression of this gene in a variety of tissues and under a variety of environmental conditions. Kranz et al were unable to detect the expression of G1331 in any tissue or treatments tested (supra).

Closely Related Genes from Other Species

G1331 shows sequence similarity with a protein from alfalfa (BF644787).

Experimental Observations

The function of G1331 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1331 in Arabidopsis did not result in any biochemical phenotypic alteration. However, G1331 overexpression produced highly pleiotropic developmental effects including; changes in leaf morphology, anthocyanin accumulation, inflorescence abnormalities, and a reduction in overall plant size. In addition, overexpression of G1331 also resulted in seedlings with an altered response to light. In a germination assay conducted in darkness, G1331 seedlings showed opened cotyledons in all three lines.

G1331 was expressed at low levels in shoots, roots, rosette leaves, and siliques. G1331 was induced by heat and SA.

Potential Applications

G1331 modifies light response and thus this gene or its equivalogs may be useful for modifying plant growth or development, for example, photomorphogenesis in poor light, or accelerating flowering time in response to various light intensities, quality or duration to which a non-transformed plant would not similarly respond. Elimination of shading responses may allow increased planting densities with subsequent yield enhancement.

G1332 (SEQ ID NO: 265)

Published Information

G1332 is a member of the (R1)R2R3 subfamily of myb transcription factors. G1332 corresponds to the gene MYB82 (Kranz et al. (1998) Plant J. 16: 263-276).

Experimental Observations

The function of G1332 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1332 produced a reduction in trichome density on leaf surfaces and inflorescence stems in Arabidopsis. No other phenotypic alterations were observed in the G1332 overexpressors.

G1332 was expressed ubiquitously and may have been repressed by Erysiphe infection.

Potential Applications

The potential utility of this gene or its equivalogs is to alter trichome initiation and number in a plant. It would be of great agronomic value to have plants that produce greater numbers of glandular trichomes that produce valuable essential oils for the pharmaceutical and food industries, as well as oils that protect plants against insect and pathogen attack.

G1363 (SEQ ID NO: 267)

Published Information

G1363 was identified based on its similarity to other members of the Hap2-like CCAAT-box binding factors. The gene was found in the sequence of BAC MDC16, GenBank accession number AB019229, released by the Arabidopsis Genome Initiative.

Experimental Observations

The complete sequence of G1363 was determined. The function of this gene was then analyzed using transgenic plants in which G1363 was expressed under the control of the 35S promoter. Transformants were morphologically indistinguishable from wild-type plants. Biochemical analysis of one line indicated the seeds had higher 16:0 in fatty acid content. In addition, plants overexpressing G1363 showed fewer disease symptoms following infection with the necrotrophic fungal pathogen Fusarium oxysporum compared to control plants. The experiment was repeated on individual lines, and all three lines showed the phenotype. Wild-type control plants were smaller in the repeat experiment, which could affect the disease severity of these plants and accentuate the degree of tolerance in the overexpressors.

RT-PCR analyses of the endogenous levels of G1363 indicated that this gene was expressed in all tissues and under all conditions tested.

Potential Applications

Since G1363 activity has been shown to affect the response of transgenic plants to the fungal pathogen Fusarium oxysporum, G1363 or its equivalogs could be used to manipulate the defense response in order to generate pathogen-resistant plants.

G1411 (SEQ ID NO: 269)

Published Information

G1411 was identified in the sequence of TAC clone K22G18 (GenBank accession number AB022212).

Experimental Observations

The complete sequence of G1411 was determined. The function of G1411 was analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter. G1411 overexpressing plants were smaller than wild-type controls and showed reduced apical dominance: axillary shoots develop prematurely amongst primary rosette leaves, resulting in a bushy plant. G1411 overexpressing plants behaved like the corresponding wild-type controls in all physiological and biochemical assays that were performed.

Potential Applications

G1411 or its equivalogs could be used to manipulate plant architecture.

G1417 (SEQ ID NO: 271)

Published Information

G1417 corresponds to gene AT4g01720 (CAB77742).

Closely Related Genes from Other Species

G1417 shows sequence similarity, outside of the conserved WRKY domain, with a rice protein (gi8467950).

Experimental Observations

The function of G1417 was studied using a line homozygous for a T-DNA insertion in the gene. The T-DNA insertion lies immediately upstream of the conserved WRKY domain coding sequence, and was expected to result in a null mutation. G1417 knockout mutant plants showed reduced seedling vigor during germination. The G1417 knockout showed alterations in seed fatty acid composition. An increase in 18:2 fatty acid and a decrease in 18:3 fatty acid were observed in two seed batches.

G1417 was ubiquitously expressed and did not appear to be significantly induced by any of the conditions tested.

Potential Applications

G1417 or its equivalogs could be useful to manipulate the saturation levels of lipids in seeds. Alteration in seed lipid saturation could be used to improve the heat stability of oils or to improve the nutritional quality of seed oil.

G1419 (SEQ ID NO: 273)

Published Information

G1419 was identified in the sequence of P1 clone MWD22; it corresponds to gene MWD22.13 (GenBank PID BAA97381).

Closely Related Genes from Other Species

G1419 is most closely related to some non-Arabidopsis AP2/EREBP proteins that have been suggested to be involved in the ethylene response, like tobacco EREBP-4.

Experimental Observations

To investigate the function(s) of G1419, this gene was expressed under the control of the 35S promoter in transgenic plants. G1419 overexpressing plants were essentially indistinguishable from wild-type controls in all assays performed. Two T2 lines showed altered biochemical phenotypes that were different in each one of them: One line had higher 16:0 when assayed for seed frames, and another line had higher seed protein.

G1419 appeared to be ubiquitously expressed.

Potential Applications

G1419 or its equivalogs could be used to increase seed protein, which is very important for the nutritional value and production of various food products.

G1449 (SEQ ID NO: 275)

Published Information

G1449 is annotated in the sequence of genomic clone MKP6, GenBank accession number AB022219, released by the Arabidopsis Genome Initiative.

Experimental Observations

A cDNA clone corresponding to G1449 was isolated from an embryo cDNA library. It was later identified in the sequence of genomic clone MKP6, GenBank accession number AB022219, released by the Arabidopsis Genome Initiative.

G1449 was expressed at high levels in embryos and siliques, and at significantly lower levels in roots and seedlings. It was induced by auxin in leaf tissue. Plants overexpressing G1449 showed floral abnormalities. Primary transformants showed changes in floral organ number and identity. Large petals were noted in one plant. Affected lines were also somewhat smaller than controls. These plants produced little seed and it was necessary to bulk seed for analysis. One T3 line produced flowers that were somewhat larger than control flowers with petals that were more open. These flowers often had extra petals. G1449 mutant plants did not show any other phenotypic alterations in any of the physiological or biochemical assays performed.

Potential Applications

Because larger and more open petals are produced in some G1449 overexpressing plants, G1449 or its equivalogs may be useful for modifying flower form and size in ornamental plants. The promoter of G1449 may also be useful to drive gene expression in seeds and seed pods or fruits.

G1451 (SEQ ID NO: 277)

Published Information

G1451 is ARF8, a member of the ARF class of proteins with a VP1-like N-terminal domain and a C-terminal domain with homology to Aux/IAA proteins. ARF8, like several other ARFs, contains a glutamine-rich central domain that can function as a transcriptional activation domain (1). ARF8 was shown to bind to an auxin response element (2). It was also shown that a truncated version of ARF8 lacking the DNA binding domain but containing the activation domain and the C-terminal domain could activate transcription on an auxin responsive promoter, presumably through interactions with another factor bound to the auxin response element (1). ARF8 is closely related in sequence to ARF6 (2).

Experimental Observations

G1451 was expressed throughout the plant, with the highest expression in flowers. Transcripts of G1451 were induced in leaves by a variety of stress conditions. A line homozygous for a T-DNA insertion in G1451 was used to determine the function of this gene. The T-DNA insertion of G1451 is approximately one-fifth of the way into the coding sequence of the gene and therefore is likely to result in a null mutation.

As measured by NIR, G1451 knockout mutants had increased total combined seed oil and seed protein content compared to wild-type plants.

Potential Applications

G1451 or its equivalogs may be used to alter seed oil and protein content, which may be very important for the nutritional value and production of various food products

G1451 or its equivalogs could also be used to increase plant biomass. Large size is useful in crops where the vegetative portion of the plant is the marketable portion since vegetative growth often stops when plants make the transition to flowering.

G1452 (SEQ ID NO: 279)

Published Information

G1452 was identified in the sequence of clones T22013, F12K2 with accession number AC006233 released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of G1452 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1452 produced changes in leaf development and markedly delayed the onset of flowering. 35S::G1452 plants produced dark green, flat, rounded leaves, and typically formed flower buds between 2 and 14 days later than controls. Additionally, some of the transformants were noted to have rather low trichome density on leaves and stems. At later stages of life cycle, 35S::G1452 appeared to develop slowly and senesced considerably later than wild-type controls. In addition, G1452 overexpressors were more tolerant to osmotic stress, and were insensitive to ABA in separate germination assays.

G1452 expression was not detected in any tissue tested by RT-PCR and was not induced by any environmental stress-related condition tested.

Potential Applications

On the basis of the analyses performed to date, G1452 or its equivalogs could be use to alter plant growth and development.

In addition, G1452 or its equivalogs could be used to alter a plant's response to water deficit conditions and therefore, could be used to engineer plants with enhanced tolerance to drought and salt stress.

G1463 (SEQ ID NO: 281)

Published Information

G1463 was identified in the sequence of BAC T13D8 with accession number AC004473 released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of G1463 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. In later stage plants, overexpression of G1463 resulted in premature senescence of rosette leaves. Under continuous light conditions, the most severely affected plants started to senesce approximately 10 days earlier than wild-type controls, at around 30 days after sowing. Additionally, 35S::G1463 plants formed slightly thin inflorescence stems and showed a relatively low seed yield. However, it is possible that such features directly resulted from the loss of photosynthetic capacity caused by premature senescence. 35S::G1463 transgenic plants were wild-type in phenotype with respect to the physiological and biochemical analyses performed.

G1463 expression could not be detected in any tissue or in response to environmental stress-related conditions tested using RT-PCR.

Potential Applications

On the basis of the analyses performed to date, the potential utilities of G1463 or its equivalogs could be used to manipulate senescence in plant tissues. Although leaf senescence is thought to be an evolutionary adaptation to recycle nutrients, the ability to control senescence in an agricultural setting has significant value. For example, a delay in leaf senescence in some maize hybrids is associated with a significant increase in yields and a delay of a few days in the senescence of soybean plants can have a large impact on yield. Delayed flower senescence may also generate plants that retain their blossoms longer and this may be of potential interest to the ornamental horticulture industry.

G1471 (SEQ ID NO: 283)

Published Information

G1471 was identified in the sequence of P1 clone MDK4, GenBank accession number AB010695, released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of this gene was analyzed using transgenic plants in which G1471 was expressed under the control of the 35S promoter. All 35S::G1471 primary transformants were markedly small, had narrow curled leaves and formed thin inflorescence stems. Flowers from many T1 plants were extremely poorly developed, and often had organs missing, reduced in size, or highly contorted. Due to such defects, the fertility was very low, and approximately one third of the lines were tiny and completely sterile. Plants from one T2 generation line displayed wild-type morphology, indicating that the transgene might have become silenced. Two lines, however, were small, had narrow curled leaves and flowered marginally earlier than controls. The phenotype of these transgenic plants was wild-type in all other assays performed. G1471 appeared to be expressed at medium levels in siliques and embryos.

G1471 overexpressing plants were found to have increased seed oil content compared to wild-type plants.

Potential Applications

G1471 or equivalog overexpression may be used to increase seed oil content in plants.

Because expression of G1471 is embryo and silique specific, its promoter could be useful for targeted gene expression in these tissues.

G1478 (SEQ ID NO: 285)

Published Information

G1478 was identified as a gene in the sequence of BAC Z97338, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G1478 shows some homology to non-Arabidopsis proteins within the conserved domain.

Experimental Observations

The sequence of G1478 (SEQ ID NO: 285) was determined and G1478 was analyzed using transgenic plants in which G1478 was expressed under the control of the 35S promoter. Plants overexpressing G1478 had a general delay in progression through the life cycle, in particular a delay in flowering time. Plants overexpressing G1478 also showed a increase in seed oil and an decrease in seed protein.

G1478 was expressed at higher levels in flowers, rosettes and embryos but otherwise expression was constitutive.

Potential Applications

G1478 or its equivalogs can be used to manipulate the rate at which plants grow, and flowering time.

G1478 can also be used to manipulate seed oil and protein, which can be very important from a nutritional standpoint.

G1482 (SEQ ID NO: 287)

Published Information

G1482 was identified as a gene in the sequence of BAC AC006434, released by the Arabidopsis Genome Initiative.

Experimental Observations

The sequence of G1482 was experimentally determined. The data presented for this gene are from plants homozygous for a T-DNA insertion in G1482. The T-DNA insertion of G1482 is in coding sequence and therefore this knockout mutant is likely to contain a null allele. Homozygous plants harboring a T-DNA insertion in G1482 displayed significantly more root growth on MS control plates as well as on different stresses in three separate experiments. G1482 was constitutively expressed and significantly induced by auxin, ABA and osmotic stress.

The function of G1482 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Plants overexpressing G1482 contained high levels of anthocyanins.

Potential Applications

Based on the phenotypes produced when this gene is knocked out, G1482 or its equivalogs could be used to manipulate root growth, particularly in response to environmental stresses such as drought and low nutrients.

G1482 or its equivalogs could also be used to alter anthocyanin production. The potential utilities of this gene includes alterations in pigment production for horticultural purposes, and possibly increasing stress resistance in combination with another transcription factor. Flavonoids have antimicrobial activity and could be used to engineer pathogen resistance. Several flavonoid compounds have health promoting effects such as the inhibition of tumor growth and cancer, prevention of bone loss and the prevention of the oxidation of lipids. Increasing levels of condensed tannins, whose biosynthetic pathway is shared with anthocyanin biosynthesis, in forage legumes is an important agronomic trait because they prevent pasture bloat by collapsing protein foams within the rumen. For a review on the utilities of flavonoids and their derivatives, see Dixon et al. (1999) Trends Plant Sci. 10: 394-400.

G1488 (SEQ ID NO: 289)

Published Information

G1488 was identified as a gene in the sequence of BAC F18A17 (Accession Number AC005405), released by the Cold Spring Harbor Laboratory.

Experimental Observations

The function of G1488 was analyzed using transgenic plants in which G1488 was expressed under the control of the 35S promoter. Plants from two of the three 35S::G1488 T2 populations were rather small at early stages, formed slightly rounded leaves, and produced thin bushy inflorescence stems that were shorter than those of controls. This phenotype was verified when the populations were re-grown. However, in the second sowing, plants from both lines also flowered early. Overexpression of G1488 in Arabidopsis also resulted in seedlings with an altered response to light. In a germination assay conducted in darkness, G1488 seedlings showed opened cotyledons in all three lines.

G1488 was expressed in all tissues, although it was expressed at higher levels in embryonic tissue and siliques. G1488 was slightly induced in response to ABA treatment or heat stress.

G1488 overexpressors were found to have increased seed protein content compared to wild-type plants.

Potential Applications

G1488 modified light response and thus it or its equivalogs may be useful for modifying plant growth or development, for example, photomorphogenesis in poor light, or accelerating flowering time in response to various light intensities, quality or duration to which a non-transformed plant would not similarly respond. Elimination of shading responses may allow increased planting densities with subsequent yield enhancement.

G1488 or its equivalogs could also be used to manipulate plant architecture.

G1488 or its equivalogs might be used to engineer crops with earlier flowering times. Most modern crop varieties are the result of extensive breeding programs. Many generations of backcrossing may be required to introduce desired traits. Systems that accelerate flowering could have valuable applications in such programs since they allow much faster generation times. Additionally, in some instances, a faster generation time might allow additional harvests of a crop to be made within a given growing season.

G1488 or equivalog overexpression may be used to alter seed protein content in plants

G1494 (SEQ ID NO: 291)

Published Information

The sequence of G1494 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AC006224, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis.

Experimental Observations

The complete sequence of G1494 was determined. G1494 was expressed in all tissues tested except in roots.

The function of this gene was analyzed using transgenic plants in which G1494 was expressed under the control of the 35S promoter. Overexpression of G1494 produced pleiotropic effects similar to those caused by shade avoidance responses or deficiencies in light regulated development. In particular, the 35S::G1494 phenotype was very similar to that described for plants mutant in multiple different phytochrome genes (Devlin et al. (1999) Plant Physiol. 119: 909-916), indicating that G1494 might have a role regulating or responding to light perception. Following germination, 35S::G1494 seedlings formed very long hypocotyls and displayed elongated cotyledon petioles. Rosette leaves were generally very pale, narrow, upward pointing, and had long petioles. Such effects were observed in either 12-hour or 24-hour photoperiodic conditions, and in both cases, the plants switched to flowering much earlier than wild-type controls. In 24-hour light, 35S::G1494 plants formed flower buds after making 2-4 leaves (wild type typically made 12-14 leaves), whereas in 12-hour conditions 4-7 leaves were formed (wild type typically made 25-30 leaves). In addition to this, in 35S::G1494 plants, internodes between rosette leaves extended, making a defined rosette difficult to discern. It should be noted that the inflorescences produced by these plants were uniformly extremely thin and spindly and generated very few siliques. Additionally, the seeds from one of these T2 populations were consistently large and pale compared to controls.

The morphological alterations in the 35S::G1494 plants were somewhat similar to those in the 35S::G2144 plants.

Alterations in leaf prenyl composition were consistently detected in the three 35S::G1494 lines analyzed, which could be predicted because of the morphological phenotype of 35S::G1494 overexpressors.

Potential Applications

G1494 or its equivalogs could be used to alter how plants respond to light. For example, it could be used to manipulate plant appearance, growth and development, and flowering time.

G1496 (SEQ ID NO: 293)

Published Information

The genomic sequence of G1496 has been determined as part of the Arabidopsis Genome Initiative (BAC clone T30D6, GenBank accession number AC006439).

Experimental Observations

As determined by RT-PCR, G1496 was highly expressed in rosette leaves and germinating seeds. Expression of G1496 was not induced by any stress-related treatment tested. The function of G1496 was analyzed using transgenic plants in which G1496 was expressed under the control of the 35S promoter.

Arabidopsis plants overexpressing G1496 produce more seed oil than wild-type plants.

Potential Applications

Based on the current analysis of G1496 or equivalog overexpressing plants, potential utilities for G1496 are to increase oil contents in crop plants.

G1499 (SEQ ID NO: 295)

Published Information

The sequence of G1499 was obtained from the Arabidopsis genome sequencing project, GenBank accession number AB020752, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis.

Closely Related Genes from Other Species

The similarity between G1499 and Brassica rapa subsp. pekinensis flower bud cDNA (acc#AT002234) is significant not only in the conserved bHLH domains but also outside of the domains.

Experimental Observations

The function of G1499 was analyzed using transgenic plants in which G1499 was expressed under the control of the 35S promoter. A range of phenotypes was observed in primary transformants of G1499. The most severely affected plants were smaller than controls, dark green, with strongly curled leaves, and produced bolts that terminated without an inflorescence. In some cases, flowers were replaced with filamentous structures or carpelloid structures. Less severely affected lines produced flowers where sepals were converted to carpelloid tissue. Petals and stamens were absent or reduced in size and number. Mildly affected T1 plants that were small in size but produced normal flowers were taken to the T2 generation. Three T2 lines produced plants that were smaller than controls, darker green, and had narrower leaves.

G1499 overexpressors were similar to their wild-type counterparts in all physiological and biochemical assays.

G1499 was predominantly expressed in the reproductive tissues such as flower, embryo and silique. Lower levels of expression were also detected in roots and germinating seeds. It's expression level was unaffected by any of the environmental conditions tested.

Phenotypes produced by overexpressing G1499 and G779 were similar in the aspects of flower structures. Cluster analysis using basic helix-loop-helix motif revealed that both proteins of G1499 and G779 are closely related.

Potential Applications

G1499 or its equivalogs could be used to modify plant architecture and development, including flower structure. If expressed under a flower-specific promoter, it might also be useful for engineering male sterility. Because expression of G1499 is flower and embryo specific, its promoter could be useful for targeted gene expression in these tissues.

Potential utilities of this gene or its equivalogs also include increasing chlorophyll content, allowing more growth and productivity in conditions of low light. With a potentially higher photosynthetic rate, fruits could have higher sugar content. Increased carotenoid content could be used as a nutraceutical to produce foods with greater antioxidant capability.

G1519 (SEQ ID NO: 297)

Published Information

G1519 corresponds to PEX10, which encodes a peroxisome assembly protein (Schumann et al. (1999) Plant Physiol. 119: 1147.

Closely Related Genes from Other Species

G1519 has a homolog in tomatoes (Accession # BE436-498).

Experimental Observations

The function of G1519 was analyzed by knockout analysis. Plants heterozygous for a knockout mutation in G1519 segregate 3 viable:1 inviable seeds in the silique. Homozygous G1519 knockout plants could not be obtained, due to the embryo lethality of the mutation, so no physiology or biochemistry assays could be done. G1519 is an essential gene that is necessary for embryo development.

Potential Applications

Because a knockout mutation in G1519 results in embryo lethality, the gene or its equivalogs are potentially useful as herbicide targets.

G1526 (SEQ ID NO: 299)

Published Information

The transcription regulator G1526 was identified by amino acid sequence similarity to proteins of the SWI/SNF family of chromatin remodeling factors. G1526 is found in the sequence of the chromosome 5 P1 clone MDJ22 (GenBank AB006699.1 GI:2351064), released by the Arabidopsis Genome Initiative. The translational stop codon was incorrectly predicted.

Experimental Observations

RT-PCR analysis of the endogenous level of G1526 transcripts reveals that G1526 was expressed constitutively in all Arabidopsis tissues, except in germinating seeds where no G1526 is detectable. The G1526 null mutant had higher seed oil content.

Potential Applications

G1526 or its equivalogs may be used to increase seed oil in plant seed, which might be used to increase seed oil yield, and increase the caloric content of food for humans and animal feeds.

G1540 (SEQ ID NO: 301)

Published Information

G1540 is the Arabidopsis WUSCHEL (WUS) gene and encodes a novel subclass of homeodomain protein (Mayer et al. (1998) Cell 95:805-815).

WUS is a key developmental protein that has a core role in regulating the fate of stem cells within Arabidopsis apical meristems. The central zone of an apical meristem contains a pool of undifferentiated pluripotent stems cells. These stem cells are able to both maintain themselves and supply cells for incorporation into new organs on the periphery of the meristem (shoot meristems initiate leaves whereas flower meristems initiate whorls of floral organs).

Defects are visible in the shoots and flowers of wus mutants (Laux et al. (1996) Development 122: 87-96; Endrizzi et al. (1996) Plant J. 10:967-979). Wus mutants fail to properly organize a shoot meristem in the developing embryo. Postembryonically, wus shoot meristems become flattened and terminate growth prematurely. Leaf primordia and secondary shoots often initiate ectopically across the surface of these terminated structures. The leaf primordia usually develop into a disorganized bunch and a secondary shoot meristem takes over growth. This secondary meristem then terminates and the developmental pattern is repeated, leading to a plant with no clear main axis of growth and clusters of leaves at the tips of shoots. Wus floral meristems exhibit a comparable phenotype to the shoot meristem; development often ceases prematurely such that flowers either lack the innermost whorls of organs, or possess a single stamen in place of the inner whorls.

The mutant phenotype indicates that wus is required to maintain the identity of the central zone within apical meristems and prevent those cells from becoming differentiated. In situ expression patterns of WUS RNA support such a conclusion; WUS is first observed in the embryonic shoot meristem at the 16-cell stage. Later, expression becomes confined to small groups of cells (in shoot and floral meristems) at the base of the central zone where it specifies the fate of overlying cells as stem cells. WUS is thought to be expressed, and act, independently of another homeobox gene, SHOOT MERISTEMLESS (STM), G431, which has a related function (Long et al. (1996) Development 125:3027-3035). STM is initially required for the establishment of the shoot meristem during embryogenesis. Later STM is expressed throughout the whole meristem dome where, together with an antagonist, CLAVATA1, it regulates transition of cells from the central zone towards differentiation and organ formation at the meristem periphery (Clarke et al. (1996) Development 122: 1565-1575; Endrizzi et al. (1996) Plant J. 10:967-979). A current hypothesis is that WUS specifies the identity of central stem cells whereas STM allows the progeny of those cells to proliferate before being partitioned into organ primordia (Mayer et al. (1998) Cell 95:805-815).

The effects of WUS over-expression have not yet been published. However, based on the present model for WUS function, its ectopic expression might be expected to induce formation of ectopic meristematic stem cells.

Experimental Observations

Over-expressers for G1540 (WUSCHEL) formed callus-like structures on leaves, stems and floral organs. These observations correlate with the proposed role of WUS in specifying stem cell fate in meristems. In T1 over-expressers, cells took on characteristics of stem cells at inappropriate locations, indicating that WUS was sufficient to specify stem cell identity.

Potential Applications

The over-expression phenotype indicates that G1540 is sufficient to confer stem cell identity on plant cells, and thereby prevent them from differentiating. The gene or its equivalogs might be of utility in the maintenance of plant cell lines grown in vitro, where the differentiation of those lines creates difficulties. The gene or its equivalogs might also be applied to transformation systems for recalcitrant species, where generation of callus is currently problematic but is required as part of the transformation procedure.

G1543 (SEQ ID NO: 303)

Published Information

G1543 was identified as a novel homeobox gene within section 3 of 255 from the complete sequence of Chromosome II (GenBank accession number AC005560, released by the Arabidopsis Genome Initiative).

Closely Related Genes from Other Species

The G1543 protein is related to a number of HD-ZIP proteins from other species, including OSHOX3 (AAD37696) from rice, with which sequence identity extends beyond the conserved homeodomain.

Experimental Observations

The ends of G1543 were determined by RACE and a full-length cDNA was isolated by PCR from mixed cDNA. The encoded 275 amino acid product was found to be a member the HD-ZIP class II group of HD proteins. The public annotation for this gene was incorrect; the protein predicted in the BAC report was only 162 amino acids in length.

RT-PCR analysis revealed that G1543 was expressed ubiquitously but was up-regulated in response to auxin applications.

The function of G1543 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G1543 Arabidopsis plants exhibited a range of phenotypes; most consistently, however, the plants possessed dark green leaves and an altered branching pattern that led to a shorter more compact stature. These morphological phenotypes, along with the expression data, implicate G1543 as a component of a growth or developmental response to auxin.

Biochemical assays reflected the changes in leaf color noted during morphological analysis. All three T2 lines examined displayed increased levels of leaf chlorophylls and carotenoids. Additionally, one of three lines had a decrease in seed oil combined with an increase in seed protein. A repeat experiment verified the altered seed oil and protein composition in two lines.

Physiological assays identified no clear differences between 35S::G1543 and wild-type plants.

Potential Applications

The altered levels of chlorophylls, carotenoids, seed oils, and proteins that resulted from overexpression of the gene in Arabidopsis indicate that G1543 or its equivalogs or its equivalogs might used to manipulate the composition of these substances in seed, with applications toward the improvement in the nutritional value of foodstuffs (for example, by increasing lutein).

Enhanced chlorophyll and carotenoid levels could also improve yield in crop plants. For instance lutein, like other xanthophylls such as zeaxanthin and violaxanthin, is an essential component in the protection of the plant against the damaging effects of excessive light. Specifically, lutein contributes, directly or indirectly, to the rapid rise of non-photochemical quenching in plants exposed to high light. Crop plants engineered to contain higher levels of lutein could therefore have improved photo-protection, possibly leading to less oxidative damage and better growth under high light. Additionally, elevated chlorophyll levels might increase photosynthetic capacity.

G1543 or its equivalogs might be applied to modify plant stature. This could be used to produce crops that are more resistant to damage by wind and rain, or more amenable to harvest. Plants with altered stature might also be of interest to the ornamental plant market.

This gene or its equivalogs may also be used to alter oil production in seeds, which may be very important for the nutritional quality and caloric content of foods

G1634 (SEQ ID NO: 305)

Published Information

G1634 was identified in the sequence of BAC MJJ3, GenBank accession number AB005237, released by the Arabidopsis Genome Initiative.

Experimental Observations

The complete sequence of G1634 was determined. cDNA microarray analyses of the endogenous levels of G1634 indicated that this gene was primarily expressed in root and silique tissues. In addition, G1634 expression was not altered significantly in response to any of the stress-related treatments tested. The function of this gene was analyzed using transgenic plants in which G1634 was expressed under the control of the 35S promoter. The phenotype of these transgenic plants was wild-type in all assays performed.

G1634 overexpressors were found to have altered seed protein content compared to wild-type plants.

Potential Applications

G1634 or its equivalogs could be used to alter seed protein amounts which is very important for the nutritional value and production of various food products.

G1637 (SEQ ID NO: 307)

Published Information

G1637 is a member of the myb-related subfamily of Myb transcription factors. G1637 was identified in BAC clone K11J9, accession number AB012239, release by the Arabidopsis sequencing project.

Closely Related Genes from Other Species

The most related gene to G1637 is a soybean gene represented by EST AW760127.

Experimental Observations

The complete sequence of G1637 was determined. The function of this gene was analyzed using transgenic plants in which G1637 was expressed under the control of the 35S promoter. The phenotype of these transgenic plants was wild-type in all assays performed.

RT-PCR analysis of the endogenous levels of G1637 indicated that this gene was expressed in all tissues and was induced by ABA, drought, and disease-related treatments.

G1637 overexpressors had increased seed oil and decreased seed protein content compared to wild-type plants.

Potential Applications

G1637 or equivalog overexpression may be used to alter seed protein content, which may be very important for the nutritional value and production of various food products

G1640 (SEQ ID NO: 309)

Published Information

G1640 was identified in the sequence of BAC K21P3, GenBank accession number AB016872, released by the Arabidopsis Genome Initiative.

Experimental Observations

The annotation of G1640 in BAC AB016872 was experimentally confirmed. The function of this gene was then analyzed using transgenic plants in which G1640 was expressed under the control of the 35S promoter. The transgenic plants were morphologically indistinguishable from wild-type plants. They were wild-type in all physiological assays performed. Biochemical analysis indicated that overexpression of G1640 in Arabidopsis results in an increase in seed oil content and a decrease in seed protein content.

As determined by RT-PCR, G1640 was expressed in leaves, flowers, embryos and siliques. No expression of G1640 was detected in the other tissues tested, nor was the gene induced in rosette leaves by any stress-related treatment.

Potential Applications

G1640 or its equivalogs could be used to decrease seed protein and increase seed oil amounts and/or composition which is very important for the nutritional value, caloric content and production of various food products.

G1645 (SEQ ID NO: 311)

Published Information

G1645 is a member of the (R1)R2R3 subfamily of MYB transcription factors. G1645 was identified in the sequence of BAC T24P13, GenBank accession number AC006535, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G1645 shows extensive sequence similarity to MYB proteins from other plant species including tomato (AW624217), and alfalfa (AQ917084).

Experimental Observations

The function of G1645 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1645 produced marked changes in Arabidopsis leaf, flower and shoot development. These effects were observed, to varying extents, in the majority of 35S::G1645 primary transformants.

At early stages, many 35S::G1645 T1 lines appeared slightly small and most had rather rounded leaves. However, later, as the leaves expanded, in many cases they became misshapen and highly contorted. Furthermore, some of the lines grew slowly and bolted markedly later than control plants. Following the switch to flowering, 35S::G1645 inflorescences often showed aberrant growth patterns, and had a reduction in apical dominance. Additionally, the flowers were frequently abnormal and had organs missing, reduced in size, or contorted. Pollen production also appeared poor in some instances. Due to these deficiencies, the fertility of many of the 35S::G1645 lines was low and only small numbers of seeds were produced.

Overexpression of G1645 resulted in a low germination efficiency when germinated on the 32 C heat stress.

As determined by RT-PCR, G1645 was expressed in flowers, embryos, germinating seeds and siliques. No expression of G1645 was detected in the other tissues tested. G1645 expression appeared to be repressed in rosette leaves infected with the phytopathogen Erysiphe orontii.

Potential Applications

G1645 or its equivalogs could be used to alter inflorescence structure, which may have value in production of novel ornamental plants.

G1645 or equivalog activity could be used to alter a plant's response to heat stress.

G1646 (SEQ ID NO: 313)

Published Information

G1646 was identified in the BAC sequence with GenBank accession number AB007649, released by the Arabidopsis Genome Initiative.

Experimental Observations

The complete sequence of G1646 was determined. The function of this gene was analyzed using transgenic plants in which G1646 was expressed under the control of the 35S promoter. G1646 was constitutively expressed at medium levels in all tissues and environmental conditions tested.

As measured by NIR, G1646 overexpressors had altered seed oil content compared to wild-type plants.

Potential Applications

G1646 overexpression may be used to alter seed oil content, which may be very important for the nutritional value and production of various food products

G1652 (SEQ ID NO: 315)

Published Information

The sequence of G1652 was obtained from the Arabidopsis genomic sequencing project, GenBank accession number AC005617, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis.

Experimental Observations

The complete sequence of G1652 was determined. No expression of G1652 was detected in any of the untreated tissues tested. G1652 may be induced by cold treatment and Fusarium infection.

The function of this gene was analyzed using transgenic plants in which G1652 was expressed under the control of the 35S promoter. 35S::G1652 transformants were distinctly smaller and slower developing than wild-type controls, and formed rounded dark-green leaves, and short, thin, inflorescence stems. This phenotype was apparent in the majority of primary transformants and two of the three T2 lines. Small size was also noted in the physiological assays.

G1652 overexpressors had increased seed protein content compared to wild-type plants.

Potential Applications

G1652 or equivalog overexpression may be used to alter seed protein content, which may be very important for the nutritional value and production of various food products

G1652 or its equivalogs may also be useful to regulate some aspect of plant growth and development.

G1672 (SEQ ID NO: 317)

Published Information

G1672 was first identified in the sequence of the P1 clone MIK19, GenBank accession number AB013392, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

The most related gene to G1672 is a rice gene P0710E05.22 in accession BAA99435.

Experimental Observations

The full length sequence of G1672 was experimentally confirmed. The function of G1672 was analyzed using transgenic plants in which G1672 was expressed under the control of the 35S promoter.

RT-PCR analysis was used to determine the endogenous levels of G1672 in a variety of tissues and under a variety of environmental stress-related conditions. G1672 was primarily expressed at low levels in shoots, roots, flowers, embryos and siliques. No expression was detected in rosette leaves and germinated seedlings. G1672 did not show any induction under any of the different environmental conditions tested.

As measured by NIR, G1672 overexpressors had altered seed oil content compared to wild-type plants.

Potential Applications

G1672 or equivalog overexpression may be used to alter seed oil content, which may be very important for the nutritional value and production of various food products

G1677 (SEQ ID NO: 319)

Published Information

G1677 was identified in the sequence of P1 clone:MKM21, GenBank accession number AB016876, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G1677 shows extensive sequence similarity to a protein from rice (AP004114).

Experimental Observations

The function of G1677 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. The phenotype of the 35S::G1677 transgenics was wild-type in morphology.

RT-PCR analysis of the endogenous levels of G1677 indicated that this gene was expressed in most tissues tested, although at very low levels. This gene was not induced in leaf tissue in response to any stress-related condition tested.

G1677 overexpressing plants were found to have decreased seed oil and increased seed protein content compared to wild-type plants.

Potential Applications

G1677 or equivalog overexpression may be used to alter oil and seed protein content in plants.

G1749 (SEQ ID NO: 321)

Published Information

G1749 corresponds to gene At2g20350 (AAD21753).

Experimental Observations

The function of G1749 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter.

Overexpression of G1749 induced chlorosis and death of large patches of tissue in the aerial part of the plant, indicating that it might be influencing programmed cell death, perhaps in pathways that are usually part of senescence or of the disease response. At early stages of development, 35S::G1749 seedlings appeared normal. However, towards the end of the rosette phase, these plants showed disorganized phyllotaxy and displayed rather broad flat leaves with short petioles. Randomly distributed yellow specks and patches of chlorotic tissue became visible at around this time; later these patches frequently developed into sizeable senesced regions covering large portions of the leaves. Additionally, similar effects were noted in the inflorescence, affecting cauline leaves, flower buds, and siliques. In severely affected plants, the entire inflorescence tips became brown and withered away without producing seeds. These effects were displayed by almost all of the T1 plants, and were visible in two independent batches of transformants, grown several months apart in separate locations.

Lines with the strongest phenotypes were completely infertile and senesced without setting seed. Three lines with a milder phenotype, which had produced some seed, were therefore selected for further analysis. All three T2 populations displayed the phenotypes to some extent, but these were weaker than were those shown by the majority of T1 plants.

G1749 was specifically expressed in flower and silique tissues, and was not ectopically induced by any of the conditions tested.

Potential Applications

G1749 or its equivalogs could be used to trigger cell death, and therefore to influence or control processes in which cell death plays a role. For example, if G1749 is an effective and rapid switch for cell death programs, it could be used to block pathogen infection by triggering it in infected cells and block spread of the disease.

G1749 or its equivalogs could also be used to either accelerate or slow senescence of different plant organs. Although leaf senescence is thought to be an evolutionary adaptation to recycle nutrients, the ability to control senescence in an agricultural setting has significant value. For example, a delay in leaf senescence in some maize hybrids is associated with a significant increase in yields and a delay of a few days in the senescence of soybean plants can have a large impact on yield. Delayed flower senescence may also generate plants that retain their blossoms longer and this may be of potential interest to the ornamental horticulture industry.

G1750 (SEQ ID NO: 323)

Published Information

G1750 was identified in the sequence of BAC clone T13J8; it corresponds to gene At4g27950.

Experimental Observations

The function of G1750 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1750 resulted in a significant increase in oil content in seeds, as measured by NIR. The increase in seed oil content was observed in two independent T2 lines, and was not accompanied by a substantial decrease in seed protein content. Otherwise, G1750 overexpressors behaved similarly to wild-type controls in all biochemical assays performed. No alterations were detected in the T2 35S::G1750 plants in the physiological analyses that were performed.

However, overexpression of G1750 caused alterations in plant growth and development. 35S::G1750 T1 plants showed a reduction in size, and approximately 50% were extremely tiny, infertile, and sometimes had premature leaf senescence. Seed was obtained from only the T1 plants with a weaker phenotype. Given the detrimental effects of G1750 overexpression, transgenics in which the gene is regulated by a tissue specific promoter, in particular a seed specific one, could be particularly useful to study the gene's functions and utilities.

G1750 was ubiquitously expressed. G1750 expression levels may have been altered by a variety of environmental or physiological conditions including SA.

Potential Applications

G1750 or its equivalogs could be used to increase seed oil content in crop plants.

G1756 (SEQ ID NO: 325)

Published Information

G1756 corresponds to gene AT4g23550 (CAB79310).

Closely Related Genes from Other Species

G1756 shows sequence similarity with known genes from other plant species within the conserved WRKY domain.

Experimental Observations

G1756 (SEQ ID NO:325) was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1756 caused alterations in plant growth and development, reducing overall plant size and fertility. In addition, 35S::G1756 overexpressing lines showed more disease symptoms following inoculation with a low dose of the fungal pathogen Botrytis cinerea compared to the wild-type controls. G1756 was ubiquitously expressed and transcript levels were altered by a variety of environmental or physiological conditions; G1756 expression can be induced by auxin, cold, and Fusarium.

Potential Applications

As G1756 is likely to be involved in the disease response, it or its equivalogs could be used to manipulate this response.

G1765 (SEQ ID NO: 327)

Published Information

G1765 was first identified in the sequence of the BAC clone F23E6, GenBank accession number AC006580, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

A cDNA clone NF085A08EC from elicited cell culture of Medicago truncatula is closely related to G1765.

Experimental Observations

The full length sequence of G1765 was experimentally confirmed. The function of G1765 was analyzed using transgenic plants in which G1765 was expressed under the control of the 35S promoter. The phenotype of these transgenic plants was wild-type in all assays performed with the exception of biochemical assays. Alterations in the leaf cell wall polysaccharide composition were observed in plants that overexpress G1765. In one line, an increase in the percentage of rhamnose was detected. In another line, an increase in the percentage of mannose was detected. Otherwise, G1765 overexpressors behave similarly to wild-type controls in all biochemical assays performed.

RT-PCR analysis was used to determine the endogenous levels of G1765 in a variety of tissues and under a variety of environmental stress-related conditions. G1765 was primarily expressed at low levels in roots, flowers and rosette leaves. No expression was detected in shoots, embryos, siliques and germinated seedlings. RT-PCR data also indicated a moderate induction of G1765 transcripts accumulation upon auxin and Fusarium treatments.

As measured by NIR, G1765 overexpressors had altered seed oil content compared to wild-type plants.

Potential Applications

G1765 or its equivalogs overexpression may be used to alter seed oil content, which may be very important for the nutritional value and production of various food products

G1777 (SEQ ID NO: 329)

Published Information

G1777 was identified as a gene in the sequence of Arabidopsis chromosome II, section 93 using clone F7H1 (Accession Number AC007134), released by The Institute for Genomic Research.

Closely Related Genes from Other Species

G1777 shows some homology to non-Arabidopsis proteins within the conserved RING finger domain.

Experimental Observations

G1777 (SEQ ID NO: 329) was analyzed using transgenic plants in which G1777 was expressed under the control of the 35S promoter. Overexpression of G1777 in Arabidopsis resulted in an increase in seed oil content and a decrease in seed protein content in two T2 lines. G1777 was expressed in all examined tissue of Arabidopsis. G1777 was induced by auxin and ABA treatment, and by heat stress.

Potential Applications

G1777 or its equivalogs have utility in manipulating seed oil and protein content.

G1792 (SEQ ID NO: 331)

Published Information

G1792 was identified in the sequence of BAC clone K14B15 (AB025608, gene K14B15.14).

Closely Related Genes from Other Species

G1792 shows sequence similarity, outside the conserved AP2 domain, with a portion of a predicted protein from tomato, represented by EST sequence AI776626 (AI776626 EST257726 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLER19A14, mRNA sequence).

Experimental Observations

G1792 (SEQ ID NO: 331) was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G1792 plants were more tolerant to the fungal pathogens Fusarium oxysporum and Botrytis cinerea and showed fewer symptoms after inoculation with a low dose of each pathogen. This result was confirmed using individual T2 lines. The effect of G1792 overexpression in increasing tolerance to pathogens received further, incidental confirmation. T2 plants of two 35S::G1792 lines had been growing in a room that suffered a serious powdery mildew infection. For each line, a pot of six plants was present in a flat containing nine other pots of lines from unrelated genes. In either of the two different flats, the only plants that were free from infection were those from the 35S::G1792 line. This observation suggested that G1792 overexpression might be used to increase resistance to powdery mildew. Additional experiments confirmed that 35S::G1792 plants showed increased tolerance to Erysiphe. G1792 was ubiquitously expressed, but appeared to be induced by salicylic acid.

35S::G1792 overexpressing plants also showed more tolerance to growth under nitrogen-limiting conditions. In a root growth assay under conditions of limiting N, 35S::G1792 lines were slightly less stunted. In a germination assay that monitored the effect of C on N signaling through anthocyanin production on high sucrose plus and minus glutamine the 35S::G1792 lines made less anthocyanin on high sucrose plus glutamine, suggesting that the gene can be involved in the plants ability to monitor their carbon and nitrogen status.

G1792 overexpressing plants showed several mild morphological alterations: leaves were dark green and shiny, and plants bolted, subsequently senesced, slightly later than wild-type controls. Among the T1 plants, additional morphological variation (not reproduced later in the T2 plants) was observed: many showed reductions in size as well as aberrations in leaf shape, phyllotaxy, and flower development.

Potential Applications

G1792 or its equivalogs can be used to engineer pathogen-resistant plants. In addition, it can also be used to improve seedling germination and performance under conditions of limited nitrogen.

Potential utilities of this gene or its equivalogs also include increasing chlorophyll content allowing more growth and productivity in conditions of low light. With a potentially higher photosynthetic rate, fruits could have higher sugar content. Increased carotenoid content could be used as a nutraceutical to produce foods with greater antioxidant capability.

G1792 or its equivalogs could be used to manipulate wax composition, amount, or distribution, which in turn could modify plant tolerance to drought and/or low humidity or resistance to insects, as well as plant appearance (shiny leaves). In particular, it would be interesting to see what the effect of increased wax deposition on leaves of a plant like cotton would do to drought resistance or water use efficiency. A possible application for this gene might be in reducing the wax coating on sunflower seeds (the wax fouls the oil extraction system during sunflower seed processing for oil). For this purpose, antisense or co-suppression of the gene in a tissue specific manner might be useful

G1793 (SEQ ID NO: 333)

Published Information

G1793 corresponds to gene MOE17.15 (BAB02492).

Experimental Observations

The function of G1793 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1793 produced alterations in cotyledon morphology and a mild reduction in overall plant size. Eight 35S::G1793 primary transformants were obtained. Initially, these plants displayed abnormal long, elongated cotyledons. At later stages, the plants were all rather small, and in some cases slow growing, compared to controls. Inflorescences were often thin and, in 2/8 lines, carried flowers with many non-specific abnormalities, including changes in organ size and number, and poor pollen production. All T1 plants showed moderate levels of transgene expression (determined by RT-PCR).

G1793 overexpressors produced more seed oil than control plants.

G1793 expression was detected in a variety of tissues (root, flower, embryo, silique, and germinating seedling), and, except for heat stress, did not appear to be significantly induced by any of the conditions tested.

Potential Applications

G1793 or its equivalogs may be used to increase seed oil in plant seeds, which might be used to increase seed oil yield, and increase the caloric content of food for humans and animal feeds.

G1794 (SEQ ID NO: 335)

Published Information

G1794 corresponds to gene MVP7.8 (BAB10308.1).

Experimental Observations

The function of G1794 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter.

Overexpression of G1794 caused multiple alterations in plant growth and development, as well as in the plant's behavior in some of the physiological analyses that were performed.

35S::G1794 plants showed modified branching patterns, and a reduction in apical dominance, which resulted in them having a shorter, more bushy stature than wild type. Additionally, G1794 overexpression produced changes in hypocotyl development. The loss of apical dominance was noticeable at the switch to flowering, when large numbers of secondary shoots developed prematurely from axils of primary rosette leaves. In the most extreme cases, the shoots had very short internodes, giving the inflorescence a very bushy appearance. These shoots were often very thin and flowers were relatively small and poorly fertile. At later stages, many plants appeared very small and had a low seed yield compared to wild type. Similar effects on inflorescence development were noted in each of three T2 populations examined. Additionally, the T2 seedlings were noted to have long thick hypocotyls and a decrease in root length compared to controls.

Overexpression of G1794 in Arabidopsis resulted in an increase in leaf glucosinolate M39480 in three T2 lines.

In the physiological analyses, it was noted that 35S::G1794 T2 seedlings exhibited an altered hypocotyl structure, an altered light response phenotype, and an enhanced sensitivity to osmotic stress and nitrogen depletion. All G1794 overexpressing transgenic lines showed thick, bulbous hypocotyls in the seedling stage as well as partially de-etiolated phenotype, the seedling displaying open and slightly expanded cotyledons when grown in the dark. The enhanced sensitivity to osmotic stress was observed in all three G1794 transgenic lines following a root growth assay performed on high PEG containing media. Similarly, all three G1794 transgenic lines appear more sensitive to growth on nitrogen depleted media. However, in all cases the G1794 transgenic seedlings grew less vigorously than the wild-type controls and that could contribute to their enhanced sensitivity to stress in the root growth assays.

The branching and plant architecture phenotypes observed in 35S::G1794 lines resembled phenotypes observed for three other AP2/EREBP genes: G865, G1411, and G2509. These three genes form a small clade within the large AP2/EREBP family, and G1794, although not belonging to the clade, is one of the AP2/EREBP genes closest to it in the phylogenetic tree. It is thus possible that all these genes are related in function.

G1794 was ubiquitously expressed, and was induced by several stress conditions, in particular by osmotic stress.

Potential Applications

G1794 or its equivalogs could be used to manipulate plant architecture and development.

G1794 or its equivalogs could be used to alter a plant's response to water deficit conditions and therefore, could be used to engineer plants with enhanced tolerance to drought, salt stress, and freezing.

Overexpression of G1794 or its equivalogs may also induce changes in glucosinolate content.

G1794 modified light response and thus it or its equivalogs may be useful for modifying plant growth or development, for example, photomorphogenesis in poor light, or accelerating flowering time in response to various light intensities, quality or duration to which a non-transformed plant would not similarly respond. Elimination of shading responses may allow increased planting densities with subsequent yield enhancement.

G1804 (SEQ ID NO: 337)

Published Information

G1804 was identified in the sequence of BAC F9C22, GenBank accession number AC006921, released by the Arabidopsis Genome Initiative. During the course of its functional analysis, the G1804 sequence was published as the Arabidopsis ABI5 gene, which, when knocked out, causes pleiotropic effects on responses to the hormone abscisic acid (Finkelstein et al. (1990) Plant Cell 12: 599-609). In addition, G1804 was deposited in the NCBI database as DPBF1. DPBF1 is an Arabidopsis embryo bZIP transcription factor that interacts with the late embryogenesis Dc3 gene promoter of sunflower (Kim et al: (1991) Unpublished deposit in the NCBI database).

ABI5 can be induced by ABA, drought and high salt stress in embryos (Lopez-Molina, et al (2001) Proc. Natl. Acad. Sci. USA 98: 4782-4787). Its overexpression causes ABA hypersensitivity and delayed germination (Lopez-Molina, et al (2001) Proc. Natl. Acad. Sci. USA 98: 4782-4787), and it is postulated but not shown that ABI5 could be used to engineer drought hardiness into seeds or plants.

Closely Related Genes from Other Species

G1804 is likely to be a homolog of the sunflower Dc3 promoter-binding factor-1 (DPBF-1; accession number AF001453) which also interacts with the Dc3 gene promoter of sunflower (Kim et al, 1991).

Experimental Observations

The boundaries of G1804 were experimentally determined and the function of G1804 was analyzed using transgenic plants in which this gene was expressed under the control of the 35S promoter. Plants overexpressing G1804 were later flowering and more sensitive to glucose in a germination assay. G1804 appeared to be preferentially expressed in embryos and flowers, and induced by auxin treatment. The expression pattern and annotation of G1804 correlated well with the information regarding DPB1 in the NCBI database and published information on ABI5.

Potential Applications

G1804 or its equivalogs may be used to modify sugar sensing and source-sink relationships in plants.

Manipulating the sugar signal transduction pathway may lead to altered gene expression to produce plants with desirable traits. In particular, manipulation of sugar signal transduction pathways could be used to alter source-sink relationships in seeds, tubers, roots and other storage organs leading to increase in yield.

G1804 or its equivalogs may also have a utility in modifying flowering time, and the promoter of G1804 may have some utility as an embryo specific promoter.

G1818 (SEQ ID NO: 339)

Published Information

G1818 is a member of the Hap5-like subfamily of CCAAT-box binding transcription factors. G1818 was identified in the sequence of P1 clone:MBA10, GenBank accession number AB025619, released by the Arabidopsis Genome Initiative.

Experimental Observations

The complete sequence of G1818 was determined. The function of this gene was analyzed using transgenic plants in which G1818 was expressed under the control of the 35S promoter. The phenotype of these transgenic plants was wild-type in all physiological assays performed. However, overexpression of G1818 delayed the timing of flowering and produced alterations in leaf shape. The leaves appeared to be flatter than wild-type leaves at all stages of development. In addition, G1818 overexpression resulted in higher seed protein content in two out of the three lines.

G1818 expression was detected in embryo, flower and silique tissue by RT-PCR. Expression of G1818 was also detected In leaf tissue following cold and auxin treatments. However, no cold related phenotypes were observed.

Potential Applications

G1818 or its equivalogs may be used to manipulate flowering time.

Additionally, a major concern is the escape of transgenic pollen from GMOs to wild species or so-called organic crops. Genes such as G1818 or its equivalogs that prevent vegetative transgenic crops from flowering would eliminate this worry.

G1818 or its equivalogs could also be used to increase seed protein amounts and/or alter seed protein composition, which could impact yield as well as the nutritional value and production of various food products. An increase in storage proteins is desirable for example in corn seeds to increase the nutritional value of the meal. Seed proteins play a central role in human and animal diets and represent a multibillion dollar market worldwide.

G1820 (SEQ ID NO: 341)

Published Information

G1820 is a member of the Hap5 subfamily of CCAAT-box-binding transcription factors. G1820 was identified as part of the BAC clone MBA10, accession number AB025619 released by the Arabidopsis Genome sequencing project.

Closely Related Genes from Other Species

G1820 is closely related to a soybean gene represented by EST335784 isolated from leaves infected with Colletotrichum trifolii. Similarity between G1820 and the soybean gene extends beyond the signature motif of the family to a level that would suggest the genes are orthologous. Therefore the gene represented by EST335784 may have a function and/or utility similar to that of G1820.

Experimental Observations

The complete sequence of G1820 was determined. The function of this gene was analyzed using transgenic plants in which G1820 was expressed under the control of the 35S promoter. G1820 overexpressing lines showed more tolerance to salt stress in a germination assay. They also showed insensitivity to ABA, with the three lines analyzed showing the phenotype. The salt and ABA phenotypes could be related to the plants increased tolerance to osmotic stress because in a severe water deprivation assay, G1820 overexpressors are, again, more tolerant.

Interestingly, overexpression of G1820 also consistently reduced the time to flowering. Under continuous light conditions at 20-25 C, the 35S::G1820 transformants displayed visible flower buds several days earlier than control plants. The primary shoots of these plants typically started flower initiation 1-4 leaf plastochrons sooner than those of wild type. Such effects were observed in all three T2 populations and in a substantial number of primary transformants.

When biochemical assays were performed, some changes in leaf frames were detected. In one line, an increase in the percentage of 18:3 and a decrease in 16:1 were observed. Otherwise, G1820 overexpressors behaved similarly to wild-type controls in all biochemical assays performed. As determined by RT-PCR, G1820 was highly expressed in embryos and siliques. No expression of G1820 was detected in the other tissues tested. G1820 expression appeared to be induced in rosette leaves by cold and drought stress treatments, and overexpressing lines showed tolerance to water deficit and high salt conditions.

One possible explanation for the complexity of the G1820 overexpression phenotype is that the gene is somehow involved in the cross talk between ABA and GA signal transduction pathways. It is well known that seed dormancy and germination are regulated by the plant hormones abscisic acid (ABA) and gibberellin (GA). These two hormones act antagonistically with each other. ABA induces seed dormancy in maturing embryos and inhibits germination of seeds. GA breaks seed dormancy and promotes germination. It is conceivable that the flowering time and ABA insensitive phenotypes observed in the G1820 overexpressors are related to an enhanced sensitivity to GA, or an increase in the level of GA, and that the phenotype of the overexpressors is unrelated to ABA. In Arabidopsis, GA is thought to be required to promote flowering in non-inductive photoperiods. However, the drought and salt tolerant phenotypes would indicate that ABA signal transduction is also perturbed in these plants. It seems counterintuitive for a plant with salt and drought tolerance to be ABA insensitive since ABA seems to activate signal transduction pathways involved in tolerance to salt and dehydration stresses. One explanation is that ABA levels in the G1820 overexpressors are also high but that the plant is unable to perceive or transduce the signal.

G1820 overexpressors also had decreased seed oil content and increased seed protein content compared to wild-type plants

Potential Applications

G1820 affects ABA sensitivity, and thus when transformed into a plant this transcription factor or its equivalogs may diminish cold, drought, oxidative and other stress sensitivities, and also be used to alter plant architecture, and yield.

The osmotic stress results indicate that G1820 or its equivalogs could be used to alter a plant's response to water deficit conditions and can be used to engineer plants with enhanced tolerance to drought, salt stress, and freezing. Evaporation from the soil surface causes upward water movement and salt accumulation in the upper soil layer where the seeds are placed. Thus, germination normally takes place at a salt concentration much higher than the mean salt concentration of in the whole soil profile. Increased salt tolerance during the germination stage of a crop plant would impact survivability and yield.

G1820 or its equivalogs could also be used to accelerate flowering time.

G1820 or its equivalogs may be used to modify levels of saturation in oils.

G1820 or its equivalogs may be used to seed protein content.

The promoter of G1820 could be used to drive seed-specific gene expression.

Potential Applications

G1820 or equivalog overexpression may be used to alter seed protein content, which may be very important for the nutritional value and production of various food products

G1836 (SEQ ID NO: 343)

Published Information

G1836 was identified in the sequence of BAC F14123, GenBank accession number AC007399, released by the Arabidopsis Genome Initiative.

Experimental Observations

The complete sequence of G1836 was determined. The function of this gene was analyzed using transgenic plants in which G1836 was expressed under the control of the 35S promoter. Morphologically, the plants were somewhat more pale than the wild-type controls. This observation did not translate into a detectable difference in the chlorophyll a or chlorophyll b content in these transgenics (see biochemistry data). Overexpression of G1836 affected the plants' ability to tolerate high concentrations of salt in a germination assay. All of the lines showed greater expansion of the cotyledons when seeds are germinated on MS media containing high concentrations of NaCl, indicating they had more tolerance to salt stress compared to the wild-type controls. There was no enhanced tolerance to high salt in older seedlings in a root growth assay. This was not unexpected because salt tolerance in the two developmental stages in often uncoupled in nature indicating mechanistic differences.

G1836 overexpression also resulted in plants that were more drought tolerant than wild-type control plants.

Expression of G1836 was also repressed by Erysiphe orontii infection.

Potential Applications

G1836 or its equivalogs could be used to increase plant tolerance to drought tolerance and soil salinity during germination, or at the seedling stage.

G1838 (SEQ ID NO: 345)

Published Information

G1838 corresponds to gene K21L13.1 (BAA98170).

Experimental Observations

The function of G1838 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1838 caused alterations in plant growth and development: 35S::G1838 plants were smaller than wild type, often possessed curled, darker green leaves, and showed reduced fertility. 35S::G1838 primary transformants frequently displayed seedling abnormalities such as elongated cotyledons. Later, many of the lines were small, grew slowly and formed highly abnormal leaves. These structures were often narrow, darker green, contorted, or had strange horn like growths on their surfaces. Inflorescences were typically short, poorly developed, and carried infertile flowers that had small, contorted, or missing organs. Due to these deficiencies, the many of T1 plants formed very few seeds. Three lines that showed a relatively weak phenotype were selected for further study. Plants overexpressing G1838 were found to produce more seed oil than wild-type plants.

G1838 was ubiquitously expressed, and did not appear to be significantly induced by any of the conditions tested.

G1838 belongs to the AP2 subfamily of the AP2/EREBP family. It was hypothesized that genes of this subfamily would be involved in plant developmental processes (Riechmann et al. (1998) Biol. Chem. 379:633-646) which could thus explain the pleiotropic nature of the phenotypes observed in 35S::G1838 plants.

Potential Applications

G1838 or its equivalogs may be used for increasing seed oil production in plants, which would be of nutritional value for food for human consumption as well as animal feeds.

G1841 (SEQ ID NO: 347)

Published Information

G1841 corresponds to gene MPF21.9 (BAB10294). No information is available about the function(s) of G1841.

Experimental Observations

The function of G1841 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter.

Most significantly, overexpression of G1841 markedly reduced the time to flowering. This early flowering phenotype was consistently observed over multiple plantings for each of the three T2 lines, and in a majority of primary transformants. 35S::G1841 plants appeared slightly pale and had rather flat leaves compared to wild-type controls, but no other obvious morphological alterations. In continuous light conditions, 35S::G1841 plants produced flower buds up to five days earlier than wild-type controls. Interestingly, in repeat sowings, the plants actually appeared to grow slightly faster than controls: although they switched to making flower buds several days early, they had a similar number of primary rosette leaves to wild type.

In addition to showing accelerated flowering under 24 hours light, plants from all three T2 populations produced flowers up to 2 weeks earlier than controls under a 12 hour photoperiod. 35S::G1841 seed also showed a slight tolerance to heat in a germination assay compared to wild-type controls.

G1841 appeared to be specifically expressed in floral tissues (including embryo and silique), and to be ectopically induced by heat stress. That G1841 was specifically expressed in floral tissues but yet can consistently influence the floral transition when overexpressed, might appear paradoxical, but this is a phenomenon that has already been observed for other transcription factor genes, for example the Arabidopsis homeobox gene FWA (Soppe et al. (2000) Mol. Cell. 6: 791-802). That G1841 expression was induced by heat lends support to the result that G1841 overexpression can improve germination under heat stress.

Potential Applications

G1841 or its equivalogs could be used to modify flowering time (accelerating the switch to flowering by overexpression), as well as to improve seed germination under heat stress. The promoter of G1841 could be used to direct heat inducible gene expression in transgenic plants. In general, a wide variety of applications exist for systems that either lengthen or shorten the time to flowering.

G1842 (SEQ ID NO: 349)

Published Information

G1842 corresponds to F1505.2 (BAA97510). The high level of sequence similarity between G1842 and FLOWERING LOCUS C (Michaels and Amasino, 1999; Sheldon et al., 1999) has been previously described (Ratcliffe et al. (2001) Plant Physiol. 126:122-132).

Experimental Observations

G1842 was recognized as a gene highly related to Arabidopsis FLOWERING LOCUS C (FLC; Michaels et al. (1999) Plant Cell 11, 949-956; Sheldon et al. (1999) Plant Cell 11, 445-458), and to MADS AFFECTING FLOWERING 1 (Ratcliffe et al. (2001) Plant Physiol. 126:122-132). FLC acts as a repressor of flowering (Michaels et al. (1999) Plant Cell 11, 949-956; Sheldon et al. (1999) Plant Cell 11, 445-458). Similarly, G157/MAF1 can cause a delay in flowering time when overexpressed (Ratcliffe et al. (2001) Plant Physiol. 126:122-132.

The function of G1842 was studied using transgenic plants in which this gene was expressed under the control of the 35S promoter. Overexpression of G1842 reduced the time to flowering in the Columbia background. No consistent alterations were detected in 35S::G1842 plants in the physiological and biochemical analyses that were performed.

Early flowering was observed in 13/21 35S::G1842 primary transformants: under continuous light conditions, these plants produced flower buds approximately 1 week earlier than controls. A comparable phenotype was also noted in the T2 populations from each of the three lines examined. In a separate experiment, the 35S::G1842 transgene was transformed into Stockholm (a late flowering, vernalization-sensitive ecotype). A comparable result was observed to that seen for Columbia: approximately 50% of 35S::G1842 Stockholm plants flowered earlier than wild-type controls.

Although G1842 is highly related in sequence to G157, G859, and FLC, its overexpression reduced the time to flowering, whereas overexpression of G157, G859, and FLC often caused a delay in flowering. In other words, whereas the function of G157, G859, and FLC appeared to repress flowering, G1842 was an activator of that process.

Potential Applications

G1842 or its equivalogs could be used to alter flowering time.

G1843 (SEQ ID NO: 351)

Published Information

G1843 corresponds to F15O5.3 (BAA97511). There is no literature published on G1843, except our own (Ratcliffe et al. (2001) Plant Physiol. 126:122-132). G1843 belongs to a group of five Arabidopsis MADS-box genes that are highly related to FLC (G1759), a repressor of the floral transition, and that we have called MADS AFFECTING FLOWERING 1-5 (Ratcliffe et al. (2001) Plant Physiol. 126:122-132). The published report describes functional data for only MAF1 (G157), but the sequence similarity among all the members of the group is noted.

Experimental Observations

The function of G1843 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1843 caused alterations in plant growth and development, in particular a severe reduction in overall plant size, premature senescence, and early flowering. That G1843 caused an effect in flowering time was expected because of its sequence similarity to G1759 (FLC), G157 (MAF1), and G859, G1842, and G1844. However, in contrast to all these other genes, which when overexpressed can alter flowering time (either delay or accelerate, depending on the gene) without severe side effects on the plant, overexpression of G1843 was severely detrimental.

Primary transformants for 35S::G1843 were consistently small, showed stunted growth, and formed poorly developed inflorescences that yielded relatively few seeds. The most severely affected of these plants were very small, and died at early stages of development. Approximately 50% of the 35S::G1843 transformants were also markedly early flowering and displayed visible flower buds 1-7 days earlier than any of the wild-type controls. Most notably, the leaves of 35S::G1843 transformants frequently senesced prematurely. A total of six T2 lines were morphologically examined; all exhibited (to varying extents) comparable phenotypes to those observed in the T1 generation, showing premature senescence and stunted growth. Due to these deleterious effects, however, an accurate determination of flowering time was difficult to make in the T2 generation.

The deleterious effects caused by G1843 overexpression were also noted in the physiological analyses that were performed: in general, the G1843 overexpressing lines showed reduced seedling vigor and were pale compared to wild-type controls. 35S::G1843 plants behaved otherwise like wild-type controls in the physiological assays.

No alterations were detected in 35S::G1843 plants in the biochemical analyses that were performed.

G1843 was ubiquitously expressed and did not appear to be significantly induced by any of the conditions tested.

Potential Applications

G1843 or its equivalogs could be used to manipulate flowering time.

G1852 (SEQ ID NO: 353)

Published Information

G1852 was identified by amino acid sequence similarity to plant and mammalian ankyrin-repeat proteins. It is found in the sequence of the chromosome 4 BAC F15P23 (GenBank accession number AF128392.1 GI:4325336), released by the Arabidopsis Genome Initiative. The translational start and stop codons were correctly predicted. G1852 has no distinctive features other than the presence of a 33-aa repeated ankyrin element known for protein-protein interaction, in the C-terminus of the predicted protein.

Closely Related Genes from Other Species

A comparison of the amino acid sequence of G1852 with entries available from GenBank shows strong similarity with plant ankyrins of several species (Malus domestica, Solanum tuberosum, Oryza sativa, Gossypium arboreum, Medicago truncatula, Glycine max, Lycopersicon esculentum, Pinus taeda, Lotus japonicus and Gossypium hirsutum).

Experimental Observations

G1852 (SEQ ID NO:353) was analyzed through its ectopic overexpression in plants. Analysis of the endogenous level of G1852 transcripts by RT-PCR revealed expression in all tissues tested. G1852 expression was induced in response to ABA, heat and drought treatment. 35S::G1852 overexpressor plants were more tolerant to osmotic stress in a root growth assay on PEG (polyethylene glycol)-containing media compared with wild-type controls. Seedlings were larger and had more root growth.

Potential Applications

G1852 or its equivalogs can be used to alter a plant's response to water deficit conditions and therefore, be used to engineer plants with enhanced tolerance to drought, salt stress, and freezing.

G1863 (SEQ ID NO: 355)

Published Information

G1863 was identified by amino acid sequence similarity to the rice Growth-regulating-factor1 (GRF1), with a potential role in the regulation of stem growth in rice (Knapp et al (2000) Plant Physiol. 122: 695-704) It is found in the sequence of chromosome II section 199 of 255 (GenBank accession AC006919.5 GI:6598632), released by the Arabidopsis Genome Initiative. The transcription start/stop codon was correctly predicted.

Experimental Observations

Tissue distribution of G1863 transcripts reveals that this gene was expressed constitutively, but with a reduced expression level in shoots. No changes in G1863 expression was observed in the biotic/abiotic treatments examined. Physiological analysis of G1863 null mutant showed an increase in sensitivity to germination in high salt condition. The reduction in germination and seedling vigor was specific to NaCl-treated plants. G1863 null mutants responded like wild-type control in drought and osmotic essays. This phenotype was confirmed in a follow-up experiment. G1863 mutation had no apparent effect on plant development and morphology or biochemical profile.

Potential Applications

G1863 or its equivalogs could be used to modify plant tolerance to soil salinity during the germination stage.

G1880 (SEQ ID NO: 357)

Published Information

G1880 was identified in the sequence of Chromosome 2, GenBank accession number AC006532, released by the Arabidopsis Genome Initiative

Closely Related Genes from Other Species

Closely related sequences to G1880 include a putative zinc finger protein in rice (GenBank accession number 10934090), a predicted protein in tomato (9858780), and a cDNA sequence from M. truncatula (AW685627). Similarity between G1880 and these genes extends beyond the signature motif of the family to a level that would suggest the genes are orthologous.

Experimental Observations

G1880 was expressed throughout the plant, with significantly lower levels of expression in siliques. It was induced by auxin, ABA, heat, and salt, and possibly repressed by Erysiphe infection. A line homozygous for a T-DNA insertion in G1880 was used to determine the function of this gene. These plants showed fewer disease symptoms following inoculation with a low dose of the fungal pathogen Botrytis cinerea in two separate experiments. No altered phenotypes were observed in any morphological or biochemical assay.

Potential Applications

Since G1880 activity has been shown to affect the response of transgenic plants to the fungal pathogen Botrytis cinerea, G1880 could be used to manipulate the defense response in order to generate pathogen-resistant plants.

G1895 (SEQ ID NO: 359)

Published Information

G1895 was identified as a gene in the sequence of the BAC T24P13 (Accession Number AC006535), released by the Arabidopsis thaliana Genome Center.

Experimental Observations

The function of G1895 was analyzed using transgenic plants in which G1895 was expressed under the control of the 35S promoter. Overexpression of G1895 delayed the onset of flowering in Arabidopsis by around two to three weeks under continuous light conditions, although this phenotype was observed only at low frequency. In all other physiological and biochemical assays, 35S::G1895 plants appeared identical to controls. G1895 was expressed in all tissues and the highest levels of expression were found in flowers, rosette leaves, and embryos. In rosette leaves, G1895 was be induced by auxin, ABA, and by cold stress.

Potential Applications

G1895 or its equivalogs might be used to engineer plants with a delayed flowering time.

G1902 (SEQ ID NO: 361)

Published Information

G1902 corresponds to the Arabidopsis adof2 gene (Accession number AB017565).

Experimental Observations

The function of G1902 was analyzed using transgenic plants in which G1902 was expressed under the control of the 35S promoter. Overexpression of G1902 produced deleterious effects on plant growth and development. All 35S::G1902 primary transformants appeared markedly small throughout the life cycle, produced thin inflorescence stems, and showed poor fertility compared to wild type. Comparable effects were observed, to varying extents, in each of the three T2 populations examined. Additionally, plants from one of the T2 populations showed aberrant flowers with narrow perianth organs and short stamens. It is noteworthy that all three T2 populations showed an apparent deficit of kanamycin resistant seedlings, indicating that G1902 expression may have been lethal at high dosages, or that the NPT marker gene was being silenced.

35S::G1902 plants produced more seed oil than wild-type plants. G1902 was expressed in all tissues, and was induced by auxin, ABA, heat and drought stress.

Potential Applications

G1902 or its equivalogs may be used for increasing seed oil production in plants, which would be of nutritional value for food for human consumption as well as animal feeds.

G1903 (SEQ ID NO: 363)

Published Information

G1903 was identified from the Arabidopsis genomic sequence, GenBank accession number AC021046, based on its sequence similarity within the conserved domain to other DOF related proteins in Arabidopsis.

Experimental Observations

The function of this gene was analyzed using transgenic plants in which G1903 was expressed under the control of the 35S promoter. Two lines showed a significant decrease in seed protein content and an increase in seed oil content as assayed by NIR, otherwise the phenotype of these transgenic plants was wild-type in all other assays performed.

Gene expression profiling using RT/PCR showed that G1903 was expressed predominantly in flowers, however it was almost undetected in roots and seedlings. Furthermore, there was no significant effect on expression levels of G1903 after exposure to environmental stress conditions.

Potential Applications

Seed proteins play a central role in human and animal diets and represent a multibillion dollar market worldwide. G1903 or its equivalogs could be used to alter seed protein amounts and/or composition which could impact yield as well as the caloric content and the nutritional value and production of various food products. An increase in storage proteins is desirable for example in corn seeds to increase the nutritional value of the meal.

G1919 (SEQ ID NO: 365)

Published Information

G1919 was identified as a gene in the sequence of the P1 clone MBK5 (Accession Number AB005234), released by the Kazusa DNA Research Institute (Chiba, Japan).

Experimental Observations

The function of G1919 was analyzed using transgenic plants in which G1919 was expressed under the control of the 35S promoter. 35S::G1919 transformants displayed wild-type morphology at all stages of development. However, plants overexpressing G1919 showed a greater tolerance to the fungal pathogen Botrytis cinerea than control plants. This phenotype has been confirmed by repeated experiment. No other altered phenotypes were observed in any of the physiological or biochemical assays. G1919 was expressed at low levels in flowers, and at higher levels in embryos and siliques. G1919 was not significantly induced by any condition tested.

Potential Applications

Since G1919 activity has been shown to affect the response of transgenic plants to the fungal pathogen Botrytis cinerea, G1919 or its equivalogs could be used to manipulate the defense response in order to generate pathogen-resistant plants.

G1927 (SEQ ID NO: 367)

Published Information

G1927 was identified in the sequence of BAC F23M19, GenBank accession number AC007454, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G1927 showed extensive sequence similarity to a NAC protein from tomato (BG350410).

Experimental Observations

G1927 (SEQ ID NO: 367) was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1927 in Arabidopsis resulted in plants that had an altered response to pathogen. Plants overexpressing G1927 showed fewer disease symptoms following infection with the fungal pathogen Sclerotinia sclerotiorum compared with control plants. The experiment was repeated on individual lines, and all three lines showed the enhanced pathogen tolerance phenotype. G1927 expression appeared to be ubiquitous according to RT-PCR analysis.

Potential Applications

G1927 or its equivalogs can be used to manipulate the defense response in order to generate pathogen-resistant plants.

G1930 (SEQ ID NO: 369)

Published Information

G1930 was identified in the sequence of P1 clone K13N2 (gene K13N2.7, GenBank protein accession number BAA95760).

Closely Related Genes from Other Species

G1930 shows sequence similarity, outside of the conserved AP2 and ABI3 domains, to a predicted rice protein (GenBank accession number BAB21218).

Experimental Observations

The function of G1930 was studied using transgenic plants in which this gene was expressed under the control of the 35S promoter. G1930 overexpressors were more tolerant to osmotic stress conditions. The plants responded to high NaCl and high sucrose on plates with more seedling vigor compared to wild-type control plants. In addition, an increase in the amount of chlorophylls a and b in seeds of two T2 lines was detected. However, constitutive expression of G1930 also produced a variety of morphological, physiological, and biochemical alterations. 35S::G1930 T1 plants were generally small and developed spindly inflorescences. The fertility of these plants was low and flowers often failed to open or pollinate.

G1930 was ubiquitously expressed and did not appear to be induced by any of the conditions tested.

Potential Applications

G1930 or its equivalogs could be used to increase germination under adverse osmotic stress conditions, which could impact survivability and yield. This gene could also be used to regulate the levels of chlorophyll in seeds.

G1936 (SEQ ID NO: 371)

Published Information

The sequence of G1936 was obtained from the Arabidopsis genome sequencing project, GenBank accession number AB010072, based on its sequence similarity within the conserved domain to other PCF related proteins in Arabidopsis.

Experimental Observations

The function of G1936 was studied using a line homozygous for a T-DNA insertion in the gene. The DNA insertion lies shortly before the ATG start site of the coding sequence in the 5′ UTR region and is expected to result in a null mutation.

G1936 knockout mutant plants showed more disease symptoms following inoculation with the fungal pathogen Sclerotinia sclerotiorum. They also showed more disease symptoms after inoculation with a low dose of Botrytis cinerea compared to control plants.

As determined by RT-PCR, G1936 was uniformly expressed in all tissues with exception of germinating seeds. Expression level of G1936 was unchanged by any of the environmental conditions or pathogens infections tested.

Potential Applications

Since G1936 transgenic plants have an altered response to the pathogens Sclerotinia sclerotiorum and Botrytis cinerea, G1936 or its equivalogs could be used to manipulate the defense response in order to generate pathogen-resistant plants

G1944 (SEQ ID NO: 373)

Published Information

The sequence of G1944 was obtained from EU Arabidopsis sequencing project, GenBank accession number AL049638, based on its sequence similarity within the conserved domain to other AT-Hook related proteins in Arabidopsis.

Closely Related Genes from Other Species

G1944 protein shares a significant homology to Glycine max cDNA clones. Similarity between G1944 and the Glycine max cDNA clones extends beyond the signature motif of the family to a level that would suggest the genes are orthologous. Therefore the gene represented by cDNA clones BE822274 and BE555817 may have a function and/or utility similar to that of G1944. No further information is available about the cDNA clones BE822274 and BE555817.

Experimental Observations

The sequence of G1944 was experimentally determined and the function of G1944 was analyzed using transgenic plants in which G1944 was expressed under the control of the 35S promoter.

Overexpression of G1944 reduced overall plant size and resulted in premature senescence of rosette leaves.

Physiological assays revealed that seedlings from G1944 overexpressor lines were more severely stunted in an ethylene insensitivity assay when compared to the wild-type controls. This result indicated that G1944 is involved in the ethylene signal transduction pathway. It is well known that ethylene is involved in the senescence process and therefore, the phenotype of premature senescence of rosette leaves could be related to a general sensitivity to ethylene signal transduction pathway.

As determined by RT-PCR, G1944 was expressed in most of tissues tested. Expression level of G1944 appeared to be induced by auxin and salicylic acid treatments.

Potential Applications

G1944 or its equivalogs, because of its effect on plant size and leaf senescence may be used to modify plant growth and development.

G1944 or its equivalogs could be used to alter senescence of different plant organs. Although leaf senescence is thought to be an evolutionary adaptation to recycle nutrients, the ability to control senescence in an agricultural setting has significant value. For example, a delay in leaf senescence in some maize hybrids is associated with a significant increase in yields and a delay of a few days in the senescence of soybean plants can have a large impact on yield. Delayed flower senescence may also generate plants that retain their blossoms longer and this may be of potential interest to the ornamental horticulture industry.

G1946 (SEQ ID NO: 375)

Published Information

The heat shock transcription factor G1946 is a member of the class-A HSFs (Nover et al. (1996) Cell Stress Chaperones 1: 215-223) characterized by an extended HR-A/B oligomerization domain. G1946 is found in the sequence of the chromosome 1, BAC F5D14 (GenBank accession AC007767.3 GI:7549621), released by the Arabidopsis Genome Initiative. The translational start codon was incorrectly predicted.

Closely Related Genes from Other Species

A comparison of the amino acid sequence of G1946 with sequences available from GenBank showed strong similarity with plant HSFs of several species (Lycopersicon peruvianum, Medicago truncatula, Lycopersicon esculentum, Glycine max, Solanum tuberosum, Oryza sativa and Hordeum vulgare subsp. Vulgare).

Experimental Observations

G1946 (SEQ ID NO:375) was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1946 resulted in accelerated flowering, with 35S::G1946 transformants producing flower buds up to a week earlier than wild-type controls (24-hour light conditions). These effects were seen in 12/20 primary transformants and in two independent plantings of each of the three T2 lines. Unlike many early flowering Arabidopsis transgenic lines, which are dwarfed, 35S::G1946 transformants often reached full-size at maturity, and produced large quantities of seeds, although the plants were slightly pale in coloration and had slightly flat leaves compared to wild type. In addition, 35S::G1946 plants showed an altered response to phosphate deprivation. Seedlings of G1946 overexpressors showed more secondary root growth on phosphate-free media, when compared to wild-type control. In a repeat experiment, all three lines showed the phenotype. Overexpression of G1946 in Arabidopsis also resulted in an increase in seed glucosinolate M39501 in two T2 lines. An increase in seed oil and a decrease in seed protein were also observed in these two lines. G1946 was ubiquitously expressed, and did not appear to be significantly induced or repressed by any of the biotic and abiotic stress conditions tested, with the exception of cold, which repressed G1946 expression.

Potential Applications

G1946 or its equivalogs can be used to modify flowering time, as well as to improve the plant's performance in conditions of limited phosphate, and to alter seed oil, protein, and glucosinolate composition.

G1947 (SEQ ID NO: 377)

Published Information

The heat shock transcription factor G1947 is a member of the class-A HSFs (Nover et al. (1996) Cell Stress Chaperones 1: 215-223) characterized by an extended HR-A/B oligomerization domain. G1947 is found in the sequence of the chromosome 5 P1 clone MQD19 (GenBank accession AB026651.1 GI:4757407), released by the Arabidopsis Genome Initiative. The start codon was incorrectly predicted in the public annotation.

Experimental Observations

Analysis of the endogenous level of G1947 transcripts by RT-PCR revealed a constitutive expression, with the highest expression levels in rosette leaves and the lowest in shoots and roots. G1947 expression appeared to be induced by a variety of physiological or environmental conditions (auxin, ABA, heat, drought and osmotic stress).

A line homozygous for a T-DNA insertion in G1947 was used to analyze the function of this gene. The insertion point is 163 nucleotides downstream from the initiation codon of G1947, and therefore should result in a null mutation.

G1947 mutant plants formed inflorescences that grew for an extended period of time, and continued to generate flowers for substantially longer than wild-type controls. In G1947 mutant plants, silique development was generally poor: they were very short and contained only a few irregularly shaped seeds. Thus, the extended phase of flower production observed in G1947 knockout mutant plants might have been the result of poor fertility, because extended production of flowers and delayed floral organ abscission is often seen in sterile Arabidopsis mutants. The basis for the reduced fertility of G1947 knockout plants was not apparent from the morphology of their flowers. In addition, some inconsistent effects on seedling size were noted for G1947 knockout mutants. No size differences were noted between rosette stage G1947 knockout plants and controls, although at late stages the G1947 knockout plants appeared bushier than controls, probably due to continued growth of the inflorescence stems.

No altered phenotypes were observed for G1947 knockout plants in any of the physiological or biochemical assays performed.

Potential Applications

G1947 or its equivalogs could be used to engineer infertility in transgenic plants. G1947 may also have utility in engineering plants with longer-lasting flowers for the horticulture industry.

G1948 (SEQ ID NO: 379)

Published Information

G1948 was identified by amino acid sequence similarity to plant and mammalian ankyrin-repeat proteins. It is found in the sequence of the chromosome 2, clones T8113, T30B22 (GenBank accession number AC002535.2 GI:6598379), released by the Arabidopsis Genome Initiative. G1948 has also been referred to as CHAOS (CAO), acronym for CHLOROPHYLL A/B BINDING PROTEIN HARVESTING-ORGANELLE SPECIFIC (Klimyuk et al. (1999) Plant Cell. 11(1):87-99). The CAO protein contains ankyrin repeats in its central region, and a chromodomain in its carboxy terminal part. Chromodomains are usually found in chromatin-related proteins. However, the CAO protein was shown to be a plant-specific component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting (Klimyuk et al. (1999) Plant Cell 11:87-99).

Experimental Observations

The function of G1948 was analyzed through its ectopic overexpression in plants. Expression analysis by RT-PCR revealed a low/moderate expression level of G1948 in all above-ground tissues, in agreement with previously published observations (Klimyuk et al. (1999) Plant Cell 11:87-99). G1948 expression was not altered by any of the and biotic/abiotic treatments examined.

The characterization of G1948 overexpressor lines revealed increased seed oil content relative to wild-type plants.

Potential Applications

G1948 or its equivalogs could used to increase seed oil content, which would be of value for modifying the nutritional value and caloric content of food for human consumption as well as animal feeds, and may be of value in improving seed storage characteristics.

G1950 (SEQ ID NO: 381)

Published Information

G1950 was identified by amino acid sequence similarity to plant and mammalian ankyrin-repeat proteins. G1950 is found in the sequence of the chromosome 2 BAC T4M8 (GenBank accession number AC006284.3; nid=6598551), released by the Arabidopsis Genome Initiative. G1950 has no distinctive features other than the presence of a 33-aa repeated ankyrin element known for protein-protein interaction, in the C-terminus of the predicted protein. Amino acid sequence comparison shows similarity to Arabidopsis NPR1.

Experimental Observations

The 5′ and 3′ ends of G1950 were experimentally determined by RACE, and found to correspond to the prediction in GenBank. The analysis of the endogenous expression level of G1950, as determined by RT-PCR, revealed that expression was specific to embryos, siliques and germinating seeds (young seedlings). G1950 expression was induced upon auxin treatment, which indicated that G1950 plays an important role in seed/embryo development or other processes specific to seeds (stress-related or desiccation-related).

The function of G1950 was analyzed in transgenic plants overexpressing G1950 under the control of the 35S promoter. When compared to wild-type controls, plants overexpressing G1950 were more tolerant to infection with the necrotrophic fungal pathogen Botrytis cinerea. The experiment was confirmed using mixed and individual lines. This result indicated that G1950, an Arabidopsis ankyrin protein with similarity to NPR1, may play a similar role to NPR1 in disease pathways. Transformants were morphologically indistinguishable from wild-type plants, and showed no biochemical changes in comparison to controls.

Potential Applications

35S::G1950 overexpression in Arabidopsis or ectopic expression in leaves has been shown to affect the onset of disease following inoculation with Botrytis cinerea. Therefore, G1950 or its equivalogs could be used to manipulate the defense response in order to generate pathogen-resistant plants. Furthermore, seed or embryo-specific expression of G1950 may indicate a potential function for this gene or its equivalogs in seed development. The G1950 promoter could be useful for targeted gene expression in seeds.

G1958 (SEQ ID NO: 383)

Published Information

G1958 was identified in the sequence of BAC T5F17, GenBank accession number AL049917, released by the Arabidopsis Genome Initiative.

G1958 has also been published as PHR1. Mutants in PHR1 show reduced growth under conditions of phosphate starvation and fail to induce genes normally regulated by low phosphate concentration (Rubio et al. (2001) Genes Devel. 15: 2122-2133).

Closely Related Genes from Other Species

G1958 is a member of a subclass of GARP family members that contains a second conserved domain with a somewhat regularly spaced pattern of glutamine residues. Members of this subclass are apparent in many other plant species. A potential ortholog of G1958 is a putative transcription factor from tobacco, WREBP-1 (accession number BAA75684). G1958 is the closest Arabidopsis relative of this tobacco gene, and similarity between G1958 and WREBP-1 extends beyond the signature motifs of the family to a level that would suggest the genes are orthologous. Therefore, WREBP-1 may have a function and/or utility similar to that of G1958.

Experimental Observations

The full-length coding sequence of G1958 was determined. G1958 was found to be expressed throughout the plant with highest expression in rosette leaves, flowers, and embryos, and was induced by auxin and heat. A line homozygous for a T-DNA insertion in G1958 was used to determine the function of this gene. The T-DNA insertion of G1958 was approximately 90% into the coding sequence of the gene, within a region of amino acid sequence conservation that seems to define a subfamily of GARP proteins, and therefore is likely to result in a null mutation. The phenotype of these knockout plants was wild-type in all assays performed, except that they were smaller and showed less root growth than control plants when grown on plates. This phenotype may have been environmentally influenced as it was accentuated when seedlings were transferred to stress conditions, while in contrast, small size was not noted in the soil-grown plants. G1958 was apparently necessary for optimum growth and development. However, the subtle phenotype indicated that G1958 could be partially redundant. G1958 is a member of a small GARP subfamily with high sequence similarity, so it is possible that other homologs might have overlapping function.

G1958 knockout mutants had increased seed oil and decreased seed protein content compared to wild-type plants.

Potential Applications

G1958 or its equivalogs may be used to alter seed protein content, which may be very important for the nutritional value and production of various food products

G1958 or its equivalogs could also be used to manipulate plant growth, in particular root growth.

G2007 (SEQ ID NO: 385)

Published Information

G2007 belongs to the MYB-(R1)R2R3 family of transcription factors. G2007 corresponds to the previously described gene Myb42 (Stracke and Weisshaar, 1999; direct submission of the sequence to GenBank).

Closely Related Genes from Other Species

A myb gene from Pimpinella brachycarpa (AAF22256) is related to G2007. Similarity between G2007 and this Pimpinella myb extends beyond the signature motif of the family to a level that would suggest the genes are orthologous.

Experimental Observations

The complete sequence of G2007 was determined. The function of this gene was analyzed using transgenic plants in which G2007 was expressed under the control of the 35S promoter. The phenotype of these transgenic plants was wild-type in all biochemical and physiological assays performed. However, overexpression of G2007 resulted in a delayed switch to flowering. Under continuous light conditions, 35S::G2007 plants produced approximately twice as many primary rosette leaves as controls, and formed flower buds up to two weeks late. As a consequence of this delay in flowering, the plants also senesced later than wild type. All the plants from two independent T2 lines exhibited this phenotype, in both an initial and a repeat planting. Late flowering was also noted amongst some of the primary transformants, but the T1 generation showed considerable morphological variation, making this trait more difficult to discern.

G2007 appeared to be constitutively expressed at moderate levels in all tissues tested except germinating seeds where no expression was detected. There was no induction of G2007 in response to any environmental condition tested.

Potential Applications

G2007 or its equivalogs may be used to delay flowering in plants. In species such as sugarbeet where the vegetative parts of the plants constitute the crop and the reproductive tissues are discarded, it would be advantageous to delay or prevent flowering. Extending vegetative development could bring about large increases in yields.

Additionally, a major concern is the escape of transgenic pollen from GMOs to wild species or so-called organic crops. Genes such as G2007 or its equivalogs that prevent vegetative transgenic crops from flowering would eliminate this worry.

G2010 (SEQ ID NO: 387)

Published Information

G2010 is a member of the SBP family of transcription factors and corresponds to sp14 (Cardon et al., 1999). Expression of sp14 is up-regulated during development under both long day and short day conditions and is highly expressed in the inflorescence tissue. Expression of G2010 is localized to the rib meristem and inter-primordial regions of the inflorescence apex (Cardon et al (1999) Gene 237:91-104).

Closely Related Genes from Other Species

A gene related to G2010 is squamosa-promoter binding protein 1 from Antirrhinum majus.

Experimental Observations

The complete sequence of G2010 was determined. The function of this gene was analyzed using transgenic plants in which G2010 was expressed under the control of the 35S promoter. Overexpression of G2010 resulted in a clear reduction in time to flowering. Under continuous light conditions, at 20-25° C., three independent T2 lines of 35S::G2010 plants flowered approximately one week earlier than wild-type controls. The primary shoot of 35S::G2010 plants switched to reproductive growth after producing 5-6 rosette leaves, compared with 8-10 rosette leaves in controls. Flower buds were first visible 12-14 days after sowing in 35S::G2010 plants compared with approximately 20 days for wild type. 35S::G2010 transformants were also observed to begin senescence sooner than controls. Otherwise, plants overexpressing G2010 are wild-type in phenotype.

Expression of G2010 was not detected by RT-PCR in any of the tissues tested. G2010 was slightly induced in rosette leaves in response to heat and cold stress treatments as well as salicylic acid treatment. The expression profile for G2010 indicated that this gene is involved in a plant's transition to flowering normally and in response to stressful environmental conditions.

Potential Applications

The potential utility of a gene such as G2010 or its equivalogs is to accelerate flowering time.

G2053 (SEQ ID NO: 389)

Published Information

G2053 was identified in the sequence of BAC T27C4, GenBank accession number AC022287, released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of G2053 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2053 in Arabidopsis resulted in plants with altered osmotic stress tolerance. In a root growth assay on media containing high concentrations of PEG, G2053 overexpressors showed more root growth compared to wild-type controls.

Potential Applications

Based on the altered stress tolerance induced by G2053 overexpression, this transcription factor or its equivalogs could be used to alter a plant's response water deficit conditions and, therefore, could be used to engineer plants with enhanced tolerance to drought, salt stress, and freezing.

G2059 (SEQ ID NO: 391)

Published Information

G2059 corresponds to gene AT4g13620 (CAB78404).

Experimental Observations

The function of G2059 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G2059 plants did not show major alterations in morphology and development, and were wild-type in the physiological and biochemical analyses that were performed. However, subtle changes in rosette leaf morphology were detected in 35S::G2059 transformants: rosette leaves were slightly darker green and rather narrow compared to controls.

G2059 expression was detected by RT-PCR in root tissue, and it appeared to be ectopically induced by heat stress.

As measured by NIR, G2059 overexpressors had decreased seed oil content and increased seed protein content compared to wild-type plants.

Potential Applications

G2059 or equivalog overexpression may be used to alter seed oil content, which may be very important for the nutritional value and production of various food products

G2085 (SEQ ID NO: 393)

Published Information

G2085 was identified as a gene in the sequence of BAC AL078637, released by the Arabidopsis Genome Initiative.

Experimental Observations

The sequence of G2085 was experimentally determined and the data presented for this gene were from plants homozygous for a T-DNA insertion in G2085. The T-DNA insertion of this gene was found to be in coding sequence and therefore this knockout mutant was likely to contain a null allele of G2085.

Although G2085 was constitutively expressed throughout the plant, its expression was markedly repressed by a variety of stress conditions such as ABA, cold, osmotic stress and Erysiphe, indicating that it may be a negative regulator of stress responses in Arabidopsis.

Seed of these G2085 knockout mutants had increased size and altered color.

Potential Applications

G2085 or its equivalogs could be used to modify seed size and/or morphology, which could have an impact on yield and appearance

G2105 (SEQ ID NO: 395)

Published Information

G2105 was discovered as a gene in BAC T22K18, accession number AC010927, released by the Arabidopsis genome initiative.

Closely Related Genes from Other Species

G2105 has similarity within the conserved domain of non-Arabidopsis proteins.

Experimental Observations

The ORF boundary of G2105 (SEQ ID NO: 395) was determined and G2105 was analyzed using transgenic plants in which G2105 was expressed under the control of the 35S promoter. Two of four T2 lines examined appeared dark green and were smaller than wild type at all stages of development. Additionally, the adaxial leaf surfaces from these plants had a somewhat ‘lumpy’ appearance caused by trichomes being raised-up on small mounds of epidermal cells. Two lines of G2105 overexpressing plants had larger seed. G2105 expression was root specific and induced in leaves by auxin, abscisic acid, high temperature, salt and osmotic stress treatments.

Potential Applications

On the basis of the analyses, G2105 or its equivalogs can be used to manipulate some aspect of plant growth or development, particularly in trichome development.

In addition, G2105 or its equivalogs can be used to modify seed size and/or morphology, which can have an impact on yield.

The promoter of G2105 can have some utility as a root specific promoter.

G2110 (SEQ ID NO: 397)

Published Information

G2110 corresponds to gene F6A14.5 (AAF27095).

Closely Related Genes from Other Species

G2110 shows sequence similarity, outside of the conserved WRKY domain, with other proteins of the family from several plant species, such as AC007789_(—)9 putative WRKY DNA binding protein (gi5042446), from rice.

Experimental Observations

The function of G2110 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2110 resulted in plants with an altered salt stress response. In a root growth assay on media containing high concentrations of NaCl, G2110 overexpressing lines showed more seedling vigor, less bleaching and more root growth compared to wild-type control plants. In repeat experiments, all three lines showed the salt tolerance phenotype.

No consistent alterations in plant morphology resulted from G2110 overexpression, and 35S::G2110 plants were wild-type in the biochemical analyses that were performed.

G2110 was expressed in a variety of tissues, including flower, shoot, embryo, silique, and germinating seedling samples; its expression was not detected in leaf and root tissues. G2110 expression may have been altered by several physiological conditions, and, was ectopically induced by auxin and by heat.

Potential Applications

G2110 or its equivalogs could be used to improve plant performance under conditions of salt stress. Evaporation from the soil surface causes upward water movement and salt accumulation in the upper soil layer where the seeds are placed. Thus, germination normally takes place at a salt concentration that is higher than the mean salt concentration in the whole soil profile. Increased salt tolerance during the germination stage of a crop plant would impact survivability and yield.

G2114 (SEQ ID NO: 399)

Published Information

G2114 corresponds to gene F28P22.24 (AAF21171).

Closely Related Genes from Other Species

G2114 shows sequence similarity, outside of the conserved AP2 domain, with other proteins of the family from several plant species, such as the one from Glycine max represented by EST AW780688 s175e07.y1 Gm-c1027 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1027-7165.

Experimental Observations

The function of G2114 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Seeds from one of the T2 populations were larger than controls. This effect was apparent in seed from this population's primary transformant, but was not noted in the other T2 populations.

35S::G2114 plants were wild-type in the physiological and biochemical analyses that were performed.

G2114 expression was primarily detected in embryo, silique, and germinating seedling tissue. G2114 was not ectopically induced by any of the biotic and abiotic stress conditions tested.

Potential Applications

G2114 or its equivalogs could be used to modify seed size and/or morphology, which could have an impact on yield and appearance

G2117 (SEQ ID NO: 401)

Published Information

G2117 was identified in the sequence of BAC T6L1, GenBank accession number AC011665, released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of G2117 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter.

Plants overexpressing G2117 had altered leaf morphology, coloration, and smaller overall plant size and were generally small with short, rounded, dark green leaves that became curled later in development. These plants generated thin inflorescence stems developed a rather bushy appearance, and had reduced fertility. Overexpression of G2117 in Arabidopsis also resulted in an increase in seed glucosinolate M39497 in two T2 lines. No other phenotypic alterations were observed.

G2117 appeared to be highly expressed in roots compared to all other tissues tested.

G2117 overexpressors had increased seed protein content compared to wild-type plants.

Potential Applications

G2117 or its equivalogs may be useful for altering seed glucosinolate composition.

G2117 or equivalog overexpression may also be used to alter seed protein content, which may be very important for the nutritional value and production of various food products.

G2123 (SEQ ID NO: 403)

Published Information

G2123 corresponds to a predicted putative 14-3-3 protein in annotated BAC clone T11I11 (AC012680), from chromosome 1 of Arabidopsis.

Closely Related Genes from Other Species

Because there is a high degree of similarity among all GF14 proteins, there are several GF14 proteins from other plant species, which are closely related to G2123.

Experimental Observations

G2123 corresponds to a predicted putative 14-3-3 protein in annotated BAC clone T11I11 (AC012680), from chromosome 1 of Arabidopsis.

G2123 (SEQ ID NO: 403) was analyzed using transgenic plants in which G2123 was expressed under the control of the 35S promoter. The phenotype of these transgenic plants was wild-type in all assays performed. G2123 was expressed primarily in developing seeds and silique tissue in wild-type plants.

G2123 overexpressors produced more seed oil than wild-type plants.

Potential Applications

G2123 or its equivalogs could used to increase seed oil content, which would be of value for modifying the nutritional value and caloric content of food for human consumption as well as animal feeds, and may be of value in improving seed storage characteristics.

G2130 (SEQ ID NO: 405)

Published Information

G2130 was identified in the sequence of BAC clone F15G16 (AL132959, gene F15G16.20).

Closely Related Genes from Other Species

G2130 shows sequence similarity, outside of the conserved AP2 domain, with a protein from Medicago truncatula, represented by EST sequence AW685524.

Experimental Observations

The function of G2130 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G2130 plants showed a variety of morphological and physiological alterations. Overexpression of G2130 reduced overall plant size, resulted in premature senescence, and compromised fertility. 35S::G2130 plants were smaller than wild-type controls throughout development. At around the time of bolting, leaves developed yellow patches of senesced tissue. The inflorescences from these plants were generally very thin and carried flowers with poorly developed stamens. Many flowers senesced without pollination and failed to develop a silique.

G2130 overexpressing lines showed more seedling vigor in a heat stress tolerance germination assay compared to wild-type controls. However, no difference was detected in the heat stress response assay, which is performed on older seedlings, indicating that the phenotype could be specific for germination. G2130 overexpressing lines are also somewhat more sensitive to chilling: the plants are more chlorotic and stunted when grown at 8° C. compared to the wild-type controls. They also showed more disease symptoms following inoculation with a low dose of the fungal pathogen Botrytis cinerea.

G2130 was ubiquitously expressed and did not appear to be significantly induced by any of the conditions tested.

Potential Applications

G2130 or its equivalogs could be used to improve seed germination under heat stress.

G2133 (SEQ ID NO: 407)

Published Information

G2133 corresponds to gene F26A9.11 (AAF23336).

Closely Related Genes from Other Species

G2133 shows sequence similarity with known genes from other plant species within the conserved AP2/EREBP domain.

Experimental Observations

G2133 (SEQ ID NO: 407) was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2133 caused a variety of alterations in plant growth and development: delayed flowering, altered inflorescence architecture, and a decrease in overall size and fertility. At early stages, 35S::G2133 transformants were markedly smaller than controls and displayed curled, dark-green leaves. Most of these plants remained in a vegetative phase of development substantially longer than controls, and produced an increased number of leaves before bolting. In the most severely affected plants, bolting occurred more than a month later than in wild type (24-hour light). In addition, the plants displayed a reduction in apical dominance and formed large numbers of shoots simultaneously, from the axils of rosette leaves. These inflorescence stems had short internodes, and carried increased numbers of cauline leaf nodes, giving them a very leafy appearance. The fertility of 35S::G2133 plants was generally very low. In addition, G2133 overexpressing lines were more resistant to the herbicide glyphosate. In a repeat experiment, two lines were more tolerant while one line was wild type. G2133 expression was detected in a variety of tissues: flower, leaf, embryo, and silique samples. Its expression was altered by several conditions, including auxin treatment, osmotic stress, and Fusarium infection.

Potential Applications

G2133 or its equivalogs can be used for the generation of glyphosate resistant plants, and to increase plant resistance to oxidative stress.

G2133 or its equivalogs may also be used to delay flowering in plants.

G2138 (SEQ ID NO: 409)

Published Information

G2138 corresponds to gene F23N20.12 (AAF26022).

Experimental Observations

The function of G2138 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G2138 plants were wild-type in morphology and development. G2138 overexpressors produced more seed oil than wild-type plants.

Potential Applications

G2138 or its equivalogs could used to increase seed oil content, which would be of value for modifying the nutritional value and caloric content of food for human consumption as well as animal feeds, and may be of value in improving seed storage characteristics.

G2140 (SEQ ID NO: 411)

Published Information

The sequence of G2140 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AC011665, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis. G2140 corresponds to gene F14K14.8 (AAG52041).

Closely Related Genes from Other Species

G2140 proteins show extensive sequence similarity with a tomato ovary cDNA, TAMU Lycopersicon esculentum (AI488313) and a Glycine max cDNA clone (BE020519).

Experimental Observations

The complete sequence of G2140 (SEQ ID NO: 411) was determined. G2140 was expressed throughout the plant. It showed repression by salicylic acid and Erysiphe infection. Overexpressing G2140 in Arabidopsis resulted in seedlings that were more tolerant to osmotic stress conditions. In germination assays where seedlings were exposed to high concentrations of sucrose or NaCl, all three lines tested showed better cotyledon expansion and seedling vigor. Additionally, G2140 overexpressing plants showed insensitivity to ABA in a germination assay. In general, G2140 overexpressing plants were small and sickly with short roots when grown in Petri plates. The combination of ABA insensitivity and resistance to osmotic stress at germination had also been observed for other genes, for example, G1820 and G926. Significantly, the ABA resistance was detected in a germination assay. ABA is involved in maintaining seed dormancy, and it is possible that ABA insensitivity at the germination stage promotes germination despite unfavorable conditions.

When grown in soil, G2140 overexpressing plants displayed marked changes in Arabidopsis leaf and root morphology. All twenty of the 35S::G2140 primary transformants displayed, to various extents, leaves with upcurled margins. In the most severe cases, the leaves became highly contorted and the plants were slightly small and grew more slowly than controls. Three T1 lines that showed substantial levels of G2140 overexpression (determined by RT-PCR) were chosen for further study. The T2 seedlings from each of these lines exhibited stunted roots compared with controls. Seedlings from two of these lines also showed upcurled cotyledons. At later stages, however, some T2-plants appeared wild type. Plants from some T2-populations were rather varied in size and showed hints of leaf curling later in development. However, this effect was less severe than that seen in the T1 lines. To verify the leaf-curling phenotype, two further T2 populations were morphologically examined; seedlings from one line were found to be extremely tiny with thickened hypocotyls and short stunted roots. Such plants were too small for transfer to soil. However, another line of T2-18 showed slightly contorted cotyledons and formed severely upcurled leaves, confirming the effects seen in the T1 generation.

Potential Applications

G2140 affects ABA sensitivity, and thus when transformed into a plant this transcription factor or its equivalogs may diminish cold, drought, oxidative and other stress sensitivities, and also be used to alter plant architecture, and yield.

G2140 or its equivalogs are useful for creating plants that germinate better under conditions of high salt. Evaporation from the soil surface causes upward water movement and salt accumulation in the upper soil layer where the seeds are placed. Thus, germination normally takes place at a salt concentration much higher than the mean salt concentration in the whole soil profile. Increased salt tolerance during the germination stage of a crop plant will impact survivability and yield. In addition, G2140 or its equivalogs can be used to alter a plant's response to water deficit conditions and, therefore, can be used to engineer plants with enhanced tolerance to drought, and freezing.

G2143 (SEQ ID NO: 413)

Published Information

The sequence of G2143 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AL132976, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis. G2143 corresponds to gene F11C1_(—)170 (CAB62312).

Closely Related Genes from Other Species

G2143 protein shared extensive homology in the basic helix loop helix region with a protein encoded by Glycine max cDNA clones (AW832545, BG726819 and BG154493) and a Lycopersicon esculentum cDNA clone (BE451174). There was lower homology outside of the region.

Experimental Observations

G2143 (SEQ ID NO: 413) is a member of a clade of highly related HLH/MYC proteins that also includes G779, G1063, G1499, and G2557. All of these genes caused similar pleiotropic phenotypic effects when overexpressed, the most striking of which was the production of ectopic carpelloid tissue. These genes can be considered key regulators of carpel development. Twelve out of twenty 35S::G2143 T1 lines showed a very severe phenotype; these plants were markedly small and had narrow, curled, dark-green leaves. Such individuals were completely sterile and formed highly abnormal inflorescences; shoots often terminated in pin-like structures, and flowers were replaced by filamentous carpelloid structures, or a fused mass of carpelloid tissue. Furthermore, lateral branches usually failed to develop, and tiny patches of stigmatic tissue often formed at axillary nodes of the inflorescence. Strongly affected plants displayed the highest levels of transgene expression (determined by RT-PCR). The remaining T1 lines showed lower levels of G2143 overexpression; these plants were still distinctly smaller than wild type, but had relatively normal inflorescences and produced seed. Since the strongest 35S::G2143 lines were sterile, three lines with a relatively weak phenotype, that had produced sufficient seed for biochemical analysis, were selected for further study. T2-11 plants displayed a very mild phenotype and had somewhat small, narrow, dark green leaves. The other two T2 populations, however, appeared wild type, suggesting that transgene activity might have been reduced between the generations. Reduced seedling vigor was noted in the physiological assays. G2143 expression was detected at low levels in flowers and siliques, and at higher levels in germinating seed.

Potential Applications

G2143 or its equivalogs can be used to manipulate flower form and structure or plant fertility. One application for manipulation of flower structure can be in the production of saffron, which is derived from the stigmas of Crocus sativus.

G2144 (SEQ ID NO: 415)

Published Information

The sequence of G2144 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AL132977, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis. G2144 corresponds to gene T10K17.10 (CAB67608).

Experimental Observations

The complete sequence of G2144 was determined. G2144 was expressed at low to moderate levels throughout the plant. It was not significantly induced or repressed by any of the conditions tested.

The function of this gene was analyzed using transgenic plants in which G2144 was expressed under the control of the 35S promoter. Overexpression of G2144 in Arabidopsis produced pleiotropic morphological changes that indicate the gene might affect light regulated development, or shade avoidance responses. At the seedling stage, 35S::G2144 transformants often exhibited elongated cotyledons and hypocotyls. Later, the plants developed rather pale, narrow, flat leaves that had long petioles, and were sometimes positioned in a vertical orientation. Flowering occurred several days earlier than in wild type and inflorescence stems were typically rather thin and spindly. Interestingly, in many of the plants, inflorescence stems sporadically split open at later stages. Additionally, in some plants, large numbers of secondary leaves developed in the axils of primary rosette leaves, and occasionally, internode elongation occurred between rosette leaves. It should also be noted that fertility was often poor; flowers sometimes failed to properly open or showed contorted organs, and seed yield was low.

Morphological alterations in the 35S::G2144 plants were somewhat similar to those in the 35S::G1494 plants.

In addition, overexpression of G2144 in Arabidopsis resulted in an increase in leaf glucosinolate M39480 in two T2 lines.

Potential Applications

G2144 or its equivalogs can be used to alter how plants respond to light. For example, it may be used to manipulate plant growth and development, and flowering time.

G2144 or its equivalogs could be used to alter glucosinolate composition in plants.

G2144 or its equivalogs could also be used to alter flowering time.

G2153 (SEQ ID NO: 417)

Published Information

The sequence of G2153 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AC011437, based on its sequence similarity within the conserved domain to other AT-hook related proteins in Arabidopsis. G2153 corresponds to gene F7018.4 (AAF04888).

Closely Related Genes from Other Species

G2153 protein shows extensive sequence similarity with Oryza sativa chromosome 2 and 8 clones (AP004020 and AP003891), a Lotus japonicus cDNA (AW720668) and a Medicago truncatula cDNA clone (AW574000).

Experimental Observations

The complete sequence of G2153 was determined. G2153 was strongly expressed in roots, embryos, siliques, and germinating seed, but at low or undetectable levels in shoots, flowers, and rosette leaves. It was not significantly induced or repressed by any condition tested.

The function of this gene was analyzed using transgenic plants in which G2153 was expressed under the control of the 35S promoter. Overexpression of G2153 in Arabidopsis resulted in seedlings with an altered response to osmotic stress. In a germination assay on media containing high sucrose, G2153 overexpressors had more expanded cotyledons and longer roots than the wild-type controls. This phenotype was confirmed in repeat experiments on individual lines, and all three lines showed osmotic tolerance. Increased tolerance to high sucrose could also be indicative of effects on sugar sensing. Overexpression of G2153 produced no consistent effects on Arabidopsis morphology, and no altered phenotypes were noted in any of the biochemical assays.

Potential Applications

G2153 or its equivalogs can be used to alter a plant's response to water deficit conditions and, therefore, could be used to engineer plants with enhanced tolerance to drought, salt stress, and freezing.

G2153 or its equivalogs may also be useful for altering a plant's response to sugars.

G2155 (SEQ ID NO: 419)

Published Information

The sequence of G2155 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AC012188, based on its sequence similarity within the conserved domain to other AT-hook related proteins in Arabidopsis.

Closely Related Genes from Other Species

G2155 protein shows extensive sequence similarity with Medicago truncatula cDNA clones (BG646893 and BG647027) and a Glycine max cDNA clone (BI426899).

Experimental Observations

The complete sequence of G2155 was determined. G2155 expression was detected at low levels only in flowers and embryos. It was not induced in rosette leaves by any condition tested.

The function of this gene was analyzed using transgenic plants in which G2155 was expressed under the control of the 35S promoter. Overexpression of G2155 produced a marked delay in the time to flowering. Under continuous light conditions, 35S::G2155 transformants displayed a considerable extension of vegetative development, and typically formed flower buds about two weeks later than wild-type controls. At early stages, the plants were slightly small and had rather rounded leaves compared to wild type. However, later in development, when the leaves were fully expanded, 35S::G2155 plants became very large, dark-green, and senesced much later than controls.

In addition, overexpression of G2155 resulted in an increase in seed glucosinolate M39497 in two T2 lines. No other phenotypic alterations were observed in any of the biochemical or physiological assays.

Potential Applications

G2155 or equivalog overexpression may be used to delay flowering.

G2155 or its equivalogs could also be used to alter seed glucosinolate composition.

G2192 (SEQ ID NO: 421)

Published Information

G2192 was identified in the sequence of BAC T19F6, GenBank accession number AC002343, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G2192 is very similar to a rice gene on clone P0708G2, accession number AP001539, released as part of the rice genome sequencing project. Homology between G2192 and this rice gene extends well beyond the conserved domain and thus the two genes may be orthologs.

Experimental Observations

The annotation of G2192 in BAC AC002343 was experimentally determined. The function of this gene was analyzed using transgenic plants in which G2192 was expressed under the control of the 35S promoter. Overexpression of G2192 in Arabidopsis resulted in an decrease in 18:3 fatty acid in seeds in two T2 lines. These lines also showed changes in 16:0, 18:0 and 18:2 fatty acids.

G2192 appeared to be constitutively expressed in all tissues and environmental conditions tested.

Potential Applications

G2192 or its equivalogs may have utility to alter seed fatty acid composition, which would be of significant nutritional value.

G2295 (SEQ ID NO: 423)

Published Information

G2295 corresponds to gene K19M22.9 (BAB09634).

Experimental Observations

The function of G2295 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2295 accelerated flowering by up to one week under 24-hour light conditions. Early flowering was apparent in all plants from two independent 35S::G2295 T2 lines in each of two separate sowings. Additionally, these plants had rather flat leaves compared to wild type. In the T1 generation, five of twenty lines also flowered markedly earlier than controls.

According to the results obtained in the RT-PCR experiments, G2295 was specifically expressed in embryo and silique tissues. It was not clear whether the alterations in flowering time observed in the 35S::G2295 overexpressors reflected the true function of the gene. There have already been cases described of Arabidopsis transcription factor genes that are specifically expressed in flower-derived tissues but that can affect flowering time when their expression pattern is modified, including a homeobox gene long considered representing a true flowering time locus, FWA. Similar examples have been found (e.g., G183).

35S::G2295 plants were wild-type in the physiological and biochemical analyses that were performed.

Potential Applications

G2295 or its equivalogs could be used to modify flowering time characteristics. In addition, the promoter of the gene could be used to drive embryo/silique-specific gene expression.

G2340 (SEQ ID NO: 425)

Published Information

G2340 is a member of the (R1)R2R3 subfamily of MYB transcription factors. G2340 corresponds to gene At1g74080 (AAK54746), and is also referred to as MYB122.

Closely Related Genes from Other Species

G2340 shows sequence similarity with known genes from other plant species within the conserved Myb domain.

Experimental Observations

G2340 (SEQ ID NO: 425) was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2340 produced a spectrum of deleterious effects on Arabidopsis growth and development. 35S::G2340 primary transformants were generally smaller than controls, and at early stages some displayed leaves that were held in a vertical orientation. The most severely affected lines died at early stages. Others survived, but displayed necrosis of the blades in later rosette leaves and cauline leaves. Inflorescence development was also highly abnormal; stems were typically shorter than wild type, often ‘kinked’ at nodes, and the tissue had a rather fleshy succulent appearance. Flower buds were frequently poorly formed, failed to open and withered away without siliques developing. Additionally, secondary shoot growth frequently failed the tips of such structures sometimes senesced. Due to these abnormalities, many of the primary transformants were completely infertile. Three T1 lines with a relatively weak phenotype, which did set some seed, were selected for further study. Plants from one T2-population displayed a strong phenotype, and died early in development. The other two T2 populations were slightly small, but the effects were much weaker than those seen in the parental plants, suggesting that activity of the transgene might have become reduced between the generations. It should be noted that G2340 and G671 are part of the same clade and that they had very similar morphological phenotypes and a similar expression pattern. These two genes may have overlapping or redundant phenotypes in the plant. Small, pale seedlings with strap-like leaves that held a vertical orientation were found in the mixed line populations of 35S::G2340 transgenic seedlings when grown under sterile conditions, similar to those observed in soil grown plants in the T1 generation. The necrotic lesions observed on the T1 plants grown in soil were not observed on the plants grown in culture. The necrotic lesion phenotype is a classic lesion mimic phenotype and would suggest that G2340 is involved in cell death responses, or alternatively G2340 overexpressor plants are hypersensitive to stresses. One class of lesion mimic forms progressive lesions following an inductive stress. Lesion formation may be induced in G2340 overexpressing plants grown in culture. In addition to the morphological changes, overexpression of G2340 resulted in an extreme alteration in seed glucosinolate profile. This phenotype was observed in one line in seed from two independent plantings. According to RT-PCR analysis, G2340 was expressed primarily in roots and was slightly induced in leaf tissue in response to auxin and heat treatments.

Potential Applications

G2340 or its equivalogs can be used to engineer plants with an inducible cell death response. A gene that regulates cell death in plants can be used to induce a pathogen protective hyper-response (HR) in plants without the potentially detrimental consequences of a constitutive systemic acquired resistance (SAR). Other potential utilities include the creation of novel abscission zones or inducing death in reproductive organs to prevent the spread of pollen, transgenic or otherwise. In the case of necrotrophic pathogens that rely on dead plant tissue as a source of nutrients, prevention of cell death could confer tolerance to these diseases. Overexpression of G2340 in Arabidopsis also resulted in an extreme alteration in seed glucosinolate profile. Therefore, the gene or its equivalogs can be used to alter glucosinolate composition in plants.

G2343 (SEQ ID NO: 427)

Published Information

G2343 is a member of the R2-R3 subfamily of Myb transcription factors. The gene was identified as part of BAC T12P18, accession number AC010852, released by the Arabidopsis Genome Initiative. A cDNA sequence corresponding to G2343 was submitted to GenBank, accession number AF214116, with the gene name MYB103.

Closely Related Genes from Other Species

The most related gene to G2343 is tomato gene LETHM1 (CAA64615). Similarity between G2343 and LETHM1 extends beyond the signature motif of the family to a level that would suggest the genes are orthologs.

Experimental Observations

The complete sequence of G2343 (SEQ ID NO: 427) was determined and G2343 was analyzed using transgenic plants in which G2343 was expressed under the control of the 35S promoter. The phenotype of these transgenic plants was wild-type in all assays performed. As determined by RT-PCR, G2343 was expressed in shoots, embryos and siliques. G2343 expression was induced in rosette leaves by auxin, heat stress, and infection by Fusarium oxysporum.

As measured by NIR, G2343 overexpressors had altered seed oil content compared to wild-type plants.

Potential Applications

G2343 or equivalog overexpression may be used to alter seed oil content, which may be very important for the nutritional value and production of various food products

G2346 (SEQ ID NO: 429)

Published Information

G2346 was identified in the sequence of BAC clone T10K17, GenBank accession number AL132977, released by the Arabidopsis Genome Initiative

Closely Related Genes from Other Species

G2346 shows sequence similarity with known genes from other plant species within the conserved SBP domain.

Experimental Observations

G2346 (SEQ ID NO: 429) was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G2346 seedlings from all three T2 populations had larger cotyledons and were more advanced than controls. This indicated that the seedlings developed more rapidly that the control plants. At later stages, however, G2346 overexpressing plants showed no consistent differences from control plants. The phenotype of these transgenic plants was wild-type in all other assays performed. According to RT-PCR analysis, G2346 was expressed ubiquitously.

Potential Applications

G2346 or its equivalogs can be used to produce plants that develop more quickly, particularly at early stages. For almost all commercial crops, it is desirable to use plants that establish more quickly, since seedlings and young plants are particularly susceptible to stress conditions such as salinity or disease. Since many weeds may outgrow young crops or out-compete them for nutrients, it would also be desirable to determine means for allowing young crop plants to out compete weed species. Increasing seedling growth rate (emergence) contributes to seedling vigor and allows for crops to be planted earlier in the season with less concern for losses due to environmental factors. Early planting helps add days to the critical grain-filling period and increases yield.

G2347 (SEQ ID NO: 431)

Published Information

G2347 is a member of the SBP family of transcription factors and corresponds to sp15 (Cardon et al., 1999). Expression of sp15 is up-regulated in seedlings during development under both long day and short day conditions and is highly expressed in the inflorescence tissue. Expression of G2347 is specifically localized in the inflorescence apical meristem and young flowers (Cardon et al. (1999) Gene 237: 91-104).

Closely Related Genes from Other Species

The closest relative to G2347 is the Antirrhinum protein, SBP2 (CAA63061). The similarity between these two proteins is extensive enough to suggest they might have similar functions in a plant.

Experimental Observations

G2347 (SEQ ID NO: 431) was analyzed using transgenic plants in which G2347 was expressed under the control of the 35S promoter. Overexpression of G2347 markedly reduced the time to flowering in Arabidopsis. This phenotype was apparent in the majority of primary transformants and in all plants from two out of the three T2 lines examined. Under continuous light conditions, 35S::G2347 plants formed flower buds up a week earlier than wild type. Many of the plants were rather small and spindly compared to controls. To demonstrate that overexpression of G2347 could induce flowering under less inductive photoperiods, two T2 lines were re-grown in 12 hour conditions; again, all plants from both lines bolted early, with some initiating flower buds up to two weeks sooner than wild type. As determined by RT-PCR, G2347 was highly expressed in rosette leaves and flowers, and to much lower levels in embryos and siliques. No expression of G2347 was detected in the other tissues tested. G2347 expression was repressed by cold, and by auxin treatments and by infection by Erysiphe. G2347 is also highly similar to the Arabidopsis protein G2010. The level of homology between these two proteins suggested they could have similar, overlapping, or redundant functions in Arabidopsis. In support of this hypothesis, overexpression of both G2010 and G2347 resulted in early flowering phenotypes in transgenic plants.

Potential Applications

G2347 or its equivalogs may be used to modify the time to flowering in plants.

G2379 (SEQ ID NO: 433)

Published Information

G2379 was identified in the sequence of BAC MOP10, GenBank accession number AB005241, released by the Arabidopsis Genome Initiative.

Experimental Observations

The annotation of G2379 in BAC AB005241 was experimentally confirmed. The function of this gene was analyzed using transgenic plants in which G2379 was expressed under the control of the 35S promoter. G2379 overexpressing plants showed increased seedling vigor when grown on media containing elevated sucrose levels. This phenotype might be indicative of either altered sugar sensing or increased tolerance of osmotic stress. No altered morphological or biochemical phenotypes were observed. G2379 appeared to be constitutively expressed in all tissues and environmental conditions tested.

Potential Applications

G2379 or its equivalogs could be used to alter a plant's response to water deficit conditions and, therefore, could be used to engineer plants with enhanced tolerance to drought, salt stress, and freezing.

G2379 or its equivalogs may also be useful for altering a plant's response to sugars.

G2430 (SEQ ID NO: 435)

Published Information

G2430 was identified in the sequence of BAC F27J15, GenBank accession number AC016041, released by the Arabidopsis Genome Initiative.

Closely Related Genes from Other Species

G2430 has similarity within of the conserved GARP and response-regulator domains to non-Arabidopsis proteins.

Experimental Observations

The complete sequence of G2430 (SEQ ID NO: 435) was determined. G2430 is a member of the response regulator class of GARP proteins (ARR genes), although one of the two conserved aspartate residues characteristic of response regulators is not present. The second aspartate, the putative phosphorylated site, is retained so G2430 can have response regulator function. G2430 was specifically expressed in embryo and silique tissue. G2430 can regulate plant growth; in morphological analyses, plants overexpressing G2430 showed more rapid growth than control plants at early stages, and in two of three lines examined produced large, flat leaves. Early flowering was observed for some lines, but this effect was inconsistent between plantings.

Overexpression of G2430 in Arabidopsis resulted in seedlings that are more tolerant to heat in a germination assay. Seedlings from G2430 overexpressing transgenic plants were greener than the control seedlings under high temperature conditions. These observations were repeated in subsequent experiments.

Potential Applications

G2430 or its equivalogs may be used to create crops with better germination under hot conditions. The germination of many crops is very sensitive to temperature. A gene that would enhance germination in hot conditions may be useful for crops that are planted late in the season or in hot climates.

G2430 or its equivalogs can be used to promote faster development and reproduction in plants.

G2505 (SEQ ID NO: 437)

Published Information

G2505 was identified in the sequence of contig fragment No. 29, GenBank accession number AL161517, released by the Arabidopsis Genome Initiative.

Experimental Observations

Analysis of the function of G2505 was attempted through the generation transgenic plants in which the gene was expressed under the control of the 35S promoter. Numerous attempts were required to obtain 35S::G2505 transformants; thus, overexpression of this gene likely caused lethality during embryo or early seedling development. The transformants that were obtained exhibited improved drought stress tolerance compared to control plants.

G2505 was expressed in all tissues except shoots and rosette leaves according to RT-PCR. No induction of G2505 expression in leaf tissue was detected in response to environmental stress related conditions.

Potential Applications

G2505 or its equivalogs could be used to engineer drought hardiness into seeds or plants, thus providing for improved survival, vigor, appearance, and/or yield in drought stress conditions.

G2509 (SEQ ID NO: 439)

Published Information

G2509 corresponds to gene T2I1_(—)20 (CAB87920).

Closely Related Genes from Other Species

G865 and other non-Arabidopsis AP2/EREBP proteins were similar within the conserved AP2 domain.

Experimental Observations

G2509 (SEQ ID NO: 439) was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2509 caused multiple alterations in plant growth and development, most notably, altered branching patterns, and a reduction in apical dominance, giving the plants a shorter, more bushy stature than wild type. Twenty 35S::G2509 primary transformants were examined; at early stages of rosette development, these plants displayed a wild-type phenotype. However, at the switch to flowering, almost all T1 lines showed a marked loss of apical dominance and large numbers of secondary shoots developed from axils of primary rosette leaves. In the most extreme cases, the shoots had very short internodes, giving the inflorescence a very bushy appearance. Such shoots were often very thin and flowers were relatively small and poorly fertile. At later stages, many plants appeared very small and had a low seed yield compared to wild type. In addition to the effects on branching, a substantial number of 35S::G2509 primary transformants also flowered early and had buds visible several days prior to wild type. Similar effects on inflorescence development were noted in each of three T2 populations examined. The branching and plant architecture phenotypes observed in 35S::G2509 lines resemble phenotypes observed for three other AP2/EREBP genes: G865, G1411, and G1794, G2509, G865, and G1411 form a small clade within the large AP2/EREBP family, and G1794, although not belonging to the clade, is one of the AP2/EREBP genes closest to it in the phylogenetic tree. It is thus likely that all these genes share a related function, such as affecting hormone balance.

G2509 overexpressing plants had increased seed protein compared to wild-type control plants.

Overexpression of G2509 in Arabidopsis resulted in an increase in alpha-tocopherol in seeds in two T2 lines. G2509 was ubiquitously expressed in Arabidopsis plant tissue. G2509 expression levels were altered by a variety of environmental or physiological conditions.

Potential Applications

G2509 or its equivalogs can be used to manipulate plant architecture and development.

G2509 or its equivalogs can be used to alter tocopherol composition.

G2509 or its equivalogs can be useful in altering flowering time.

G2517 (SEQ ID NO: 441)

Published Information

G2517 corresponds to gene T12C14_(—)40 (CAB82948).

Closely Related Genes from Other Species

G2517 shows sequence similarity with known genes from other plant species within the conserved WRKY domain.

Experimental Observations

G2517 (SEQ ID NO: 441) was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2517 caused alterations in plant growth and development: size variation was apparent in the 35S::G2517 T1 generation, with at least half the lines being very small. Additionally, four of twelve T1 plants formed flower buds marginally earlier than wild type. Three T1 lines were examined in the T2 generation, and all three T2 populations were slightly smaller than controls. In the physiological analysis of the T2 populations, G2517 overexpressing lines were more resistant to the herbicide glyphosate.

Potential Applications

G2517 or its equivalogs can be used for the generation of glyphosate resistant plants, and to increase plant resistance to oxidative stress.

G2520 (SEQ ID NO: 443)

Published Information

The sequence of G2520 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AC009317, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis.

Closely Related Genes from Other Species

G2520 shows sequence similarity with known genes from other plant species within the conserved basic HLH domain.

Experimental Observations

G2520 (SEQ ID NO: 443) was analyzed using transgenic plants in which G2520 was expressed under the control of the 35S promoter. At early stages, 35S::G2520 transformants displayed abnormal curled cotyledons, long hypocotyls, and rather short roots. During the vegetative phase, these plants formed somewhat small flat leaves. Following the switch to reproductive growth, 35S::G2520 inflorescences were typically very spindly, slightly pale colored, and stems often split open at late stages. Flowers were frequently small with narrow organs and showed poor pollen production. As a result, the seed yield from 35S::G2520 plants was low compared to wild-type controls. These effects were observed in the majority of primary transformants, and to varying extents, in all three of the T2 populations. Overexpression of G2520 also resulted in an increase in the leaf glucosinolate M39478 in two lines. In addition, these lines showed an increase in seed delta-tocopherol and a decrease in seed gamma-tocopherol. No altered phenotypes were detected in any of the physiological assays. G2520 was expressed throughout the plant and was induced by ABA, heat, salt, drought and osmotic stress.

Potential Applications

G2520 or its equivalogs may be useful for manipulating plant development and altering leaf glucosinolate composition.

G2520 or its equivalogs can also be used to modify seed tocopherol composition.

G2555 (SEQ ID NO: 445)

Published Information

The sequence of G2555 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AC023064, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis. G2555 corresponds to gene At1g35460/F12A4_(—)2 (AAG52112).

Experimental Observations

The complete sequence of G2555 was determined. G2555 was expressed throughout the plant, with the highest levels being detected in shoots, flowers, rosette leaves, siliques, and germinating seed. It was not significantly induced or repressed by any condition tested.

The function of this gene was analyzed using transgenic plants in which G2555 was expressed under the control of the 35S promoter. Overexpression of G2555 in Arabidopsis resulted in a small decrease in the time to flowering. Under continuous light conditions, 35S::G2555 transformants produced flower buds and bolted approximately two to five days earlier than wild-type controls. Such effects were readily visible in seven of twenty primary transformants and all plants from two of the three T2 populations. The third T2 population had only two of six plants that flowered early.

G2555 overexpressing seedlings showed open cotyledons when grown in the dark, indicating that G2555 may affect photomorphogenesis. This phenotype could be related to the early flowering noted in morphology, if G2555 is involved in light regulation of development. G2555 plants also showed increased sensitivity to infection by the necrotrophic fungal pathogen Botrytis cinerea. In repeat experiments on individual lines, all G2555 overexpressing lines showed similar phenotypes. No altered phenotypes were detected in any of the biochemical assays.

Potential Applications

G2555 or its equivalogs may be useful for accelerating flowering time in crop plants.

Since G2555 transgenic plants have an altered response to the fungal pathogen Botrytis cinerea, G2555 or its equivalogs might be used to manipulate the defense response in order to generate pathogen-resistant plants. G2555 or its equivalogs may also be useful for altering some aspect of light-regulated development.

G2557 (SEQ ID NO: 447)

Published Information

The sequence of G2557 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AP001305, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis.

Closely Related Genes from Other Species

G2557 protein shows extensive sequence similarity in the region of basic helix loop helix with a protein encoded by Glycine max cDNA clone (BE347811).

Experimental Observations

G2557 (SEQ ID NO: 447) is a member of a clade of highly related HLH/MYC proteins that also includes G779, G1063, G1499, and G2143. All of these genes caused similar pleiotropic phenotypic effects when overexpressed, the most striking of which was the production of ectopic carpelloid tissue. These genes can be considered key regulators of carpel development. The flowers of 35S::G2557 primary transformants displayed patches of stigmatic papillae on the sepals, and often had rather narrow petals and poorly developed stamens. Additionally, carpels were also occasionally held outside of the flower at the end of an elongated pedicel like structure. As a result of such defects, 35S::G2557 plants often showed very poor fertility and formed small wrinkled siliques. In addition to such floral abnormalities, the majority of primary transformants were also small and darker green in coloration than wild type. Approximately one third of the T1 plants were extremely tiny and completely sterile. Three T1 lines that had produced some seeds and showed a relatively weak phenotype were chosen for further study. All three of the T2 populations from these lines contained plants that were distinctly small, had abnormal flowers, and were poorly fertile compared to controls. Stigmatic tissue was not noted on the sepals of plants from these three T2 lines. Another line that had shown a moderately strong phenotype in the T1 was sown for only morphological analysis in the T2 generation. These T2 plants were small, dark green, and produced abnormal flowers with ectopic stigmatic tissue on the sepals, as had been seen in the parental plant. G2557 expression was detected at low to moderate levels in all tissues tested except shoots. G2557 was induced by cold, heat, and salt, and repressed by pathogen infection

Potential Applications

G2557 or its equivalogs can be used to manipulate flower form and structure or plant fertility. One application for manipulation of flower structure can be in the production of saffron, which is derived from the stigmas of Crocus sativus.

G2583 (SEQ ID NO: 449)

Published Information

G2583 corresponds to gene F2I11_(—)80 (CAB96654).

Closely Related Genes from Other Species

G2583 showed sequence similarity with known genes from other plant species within the conserved AP2/EREBP domain.

Experimental Observations

G2583 (SEQ ID NO: 449) was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G2583 plants exhibited extremely glossy leaves. At early stages, 35S::G2583 seedlings appeared normal, but by about two weeks after sowing, the plants exhibited very striking shiny leaves, which were apparent until very late in development. Many lines displayed a variety of other effects such as a reduction in overall size, narrow curled leaves, or various non-specific floral abnormalities, which reduced fertility. These effects on leaf appearance were observed in eighteen of twenty primary transformants, and in all the plants from four of six of the T2 lines examined. The glossy nature of the leaves from 35S::G2583 plants may be a consequence of changes in epicuticular wax content or composition. G2583 belongs to a small clade within the large AP2/EREBP Arabidopsis family that also contains G975, G1387, and G977. Overexpression of G975 caused a substantial increase in leaf wax components, as well as morphological phenotypes resembling those observed in 35S::G2583 plants. G2583 was ubiquitously expressed, at higher levels in root, flower, embryo, and silique tissues.

Potential Applications

G2583 or its equivalogs can be used to modify plant appearance by producing shiny leaves. In addition, it or its equivalogs can be used to manipulate wax composition, amount, or distribution, which in turn can modify plant tolerance to drought and/or low humidity or resistance to insects.

G2701 (SEQ ID NO: 451)

Published Information

G2701 was identified in the sequence of BAC F11B9, GenBank accession number AC073395, released by the Arabidopsis Genome Initiative.

Experimental Observations

The function of G2701 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2701 is Arabidopsis resulted in plants that were wild-type in morphology and in the biochemical analyses performed. However, 35S::G2701 transgenic plants were more tolerant to osmotic stress in a germination assay, the seedlings were greener with expanded cotyledons and longer roots than wild-type controls when germinated on plates containing either high salt or high sucrose. The phenotype was repeated in all three lines.

G2701 was expressed ubiquitously in Arabidopsis according to RT-PCR, and the level of G2701 expression in leaf tissue was essentially unchanged in response to environmental stress related conditions.

Potential Applications

G2701 or its equivalogs could be used to alter a plant's response to water deficit conditions and therefore, could be used to engineer plants with enhanced tolerance to drought, salt stress, and freezing.

G2719 (SEQ ID NO: 453)

Published Information

G2719 is a member of the (R1)R2R3 subfamily of MYB transcription factors. G2719 corresponds to gene At3g55730 (AAF72669), and is also referred to as MYB109.

Experimental Observations

The function of G2719 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2719 in Arabidopsis resulted in plants with increased seedling vigor in a germination assay on media containing high sucrose. This phenotype could implicate G2719 in sugar sensing and/or osmotic stress tolerance. These observations were seen in repeat experiments. 35S::G2719 transgenic plants were wild-type in morphology and in the biochemical analyses performed.

G2719 was expressed ubiquitously in Arabidopsis according to RT-PCR, and the level of G2719 expression in leaf tissue was essentially unchanged in response to environmental stress related conditions.

Potential Applications

G2719 or its equivalogs could be used to alter a plant's response water deficit conditions and therefore, could be used to engineer plants with enhanced tolerance to drought, salt stress, and freezing.

In addition, G2719 or its equivalogs could be involved in sugar sensing pathways.

G2789 (SEQ ID NO: 455)

Published Information

The sequence of G2789 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AL162295, based on its sequence similarity within the conserved domain to other AT-hook related proteins in Arabidopsis. G2789 corresponds to gene T4C21_(—)280 (CAB82691).

Closely Related Genes from Other Species

G2789 protein shows extensive sequence similarity with Medicago truncatula cDNA clones (AL366947 and BG647144), an Oryza sativa chromosome 6 clone (AP003526) and a tomato crown gall Lycopersicon esculentum cDNA clone (BG134451).

Experimental Observations

The complete sequence of G2789 was determined. G2789 was expressed at moderate levels in roots, flowers, embryos, siliques, and germinating seeds. It was not detectable in rosette leaves or shoots. No significant induction of G2789 was observed in rosette leaves by any condition tested.

The function of this gene was analyzed using transgenic plants in which G2789 was expressed under the control of the 35S promoter. Overexpression of G2789 in Arabidopsis resulted in seedlings that are ABA insensitive and osmotic stress tolerant. In a germination assay on ABA containing media, G2789 transgenic seedlings showed enhanced seedling vigor. In a similar germination assay on media containing high concentrations of sucrose, the G2789 overexpressors also showed enhanced seedling vigor. In a repeat experiment on individual lines, all three lines showed the phenotype. The combination of ABA insensitivity and better germination under osmotic stress was also observed for G1820, G926, and G2140. It is possible that ABA insensitivity at the germination stage promoted germination despite unfavorable conditions.

Overexpression of G2789 produced alterations in leaf and flower development, and caused severe reductions in fertility. 35S::G2789 primary transformants displayed a variety of leaf abnormalities including; leaf curling, serrations, and changes in leaf shape and area. The most severely affected individuals grew slowly and were often very tiny compared with wild type. During the reproductive phase, most of the lines showed non-specific defects in flower formation; organs were frequently absent or poorly developed. As a result of such deficiencies, most of the T1 plants yielded very few seed. A comparable phenotype to that seen in the T1 was observed in two of the three T2 lines. Some plants from each of these two populations showed a somewhat attenuated phenotype, suggesting that the transgene might be becoming silenced. Plants from the third line appeared wild type in both the T1 and T2 generations.

Overexpression of G2789 in Arabidopsis did not result in any biochemical phenotypic alteration.

Potential Applications

G2789 or its equivalogs could be used to alter a plant's response to water deficit conditions and therefore, could be used to engineer plants with enhanced tolerance to drought, salt stress, and freezing.

G2830 (SEQ ID NO: 457)

Published Information

G2830 was identified in the sequence of P1 clone MFO20, GenBank accession number AB013391, released by the Arabidopsis Genome Initiative.

Experimental Observations

G2830 was primarily expressed at a low level in embryos and siliques as determined by RT-PCR analysis. Expression of G2830 was not detected in other tissues. A line homozygous for a T-DNA insertion in G2830 was used to determine the function of this gene. The T-DNA insertion of G2830 was approximately one quarter into the coding sequence of the gene and therefore is likely to result in a null mutation.

The G2830 knockouts were found to produce more seed oil than wild-type plants.

Potential Applications

G2830 or its equivalogs can used to increase seed oil content, which would be of value for modifying the nutritional value and caloric content of food for human consumption as well as animal feeds, and may be of value in improving seed storage characteristics.

Because expression of G2830 is embryo and silique specific, its promoter could be useful for targeted gene expression in these tissues.

Example IX Identification of Homologous Sequences

This example describes identification of genes that are orthologous to Arabidopsis thaliana transcription factors from a computer homology search.

Homologous sequences, including those of paralogs and orthologs from Arabidopsis and other plant species, were identified using database sequence search tools, such as the Basic Local Alignment Search Tool (BLAST) (Altschul et al. (1990) J. Mol. Biol. 215: 403-410; and Altschul et al. (1997) Nucleic Acid Res. 25: 3389-3402). The tblastx sequence analysis programs were employed using the BLOSUM-62 scoring matrix (Henikoff and Henikoff (1992) Proc Natl. Acad. Sci. 89: 10915-10919). The entire NCBI GenBank database was filtered for sequences from all plants except Arabidopsis thaliana by selecting all entries in the NCBI GenBank database associated with NCBI taxonomic ID 33090 (Viridiplantae; all plants) and excluding entries associated with taxonomic ID 3701 (Arabidopsis thaliana).

These sequences are compared to sequences representing genes of SEQ ID NO: 2N−1, wherein N=1-229, using the Washington University TBLASTX algorithm (version 2.0a19MP) at the default settings using gapped alignments with the filter “off”. For each gene of SEQ ID NO: 2N−1, wherein N=1-229, individual comparisons were ordered by probability score (P-value), where the score reflects the probability that a particular alignment occurred by chance. For example, a score of 3.6e-40 is 3.6×10-40. In addition to P-values, comparisons were also scored by percentage identity. Percentage identity reflects the degree to which two segments of DNA or protein are identical over a particular length. Examples of sequences so identified are presented in Table 7 and Table 9. Paralogous or orthologous sequences were readily identified and available in GenBank by Accession number (Table 7; Test sequence ID). The percent sequence identity among these sequences can be as low as 47%, or even lower sequence identity. Candidate paralogous sequences were identified among Arabidopsis transcription factors through alignment, identity, and phylogenic relationships. A list of paralogs is shown in Table 9. Candidate orthologous sequences were identified from proprietary unigene sets of plant gene sequences in Zea mays, Glycine max and Oryza sativa based on significant homology to Arabidopsis transcription factors. These candidates were reciprocally compared to the set of Arabidopsis transcription factors. If the candidate showed maximal similarity in the protein domain to the eliciting transcription factor or to a paralog of the eliciting transcription factor, then it was considered to be an ortholog. Identified non-Arabidopsis sequences that were shown in this manner to be orthologous to the Arabidopsis sequences are provided in Table 7.

Example X Screen of Plant cDNA Library for Sequence Encoding a Transcription Factor DNA Binding Domain that Binds to a Transcription Factor Binding Promoter Element and Demonstration of Protein Transcription Regulation Activity

The “one-hybrid” strategy (Li and Herskowitz (1993) Science 262: 1870-1874) is used to screen for plant cDNA clones encoding a polypeptide comprising a transcription factor DNA binding domain, a conserved domain. In brief, yeast strains are constructed that contain a lacZ reporter gene with either wild-type or mutant transcription factor binding promoter element sequences in place of the normal UAS (upstream activator sequence) of the GALL promoter. Yeast reporter strains are constructed that carry transcription factor binding promoter element sequences as UAS elements are operably linked upstream (5′) of a lacZ reporter gene with a minimal GAL1 promoter. The strains are transformed with a plant expression library that contains random cDNA inserts fused to the GAL4 activation domain (GAL4-ACT) and screened for blue colony formation on X-gal-treated filters (X-gal: 5-bromo-4-chloro-3-indolyl-β-D-galactoside; Invitrogen Corporation, Carlsbad Calif.). Alternatively, the strains are transformed with a cDNA polynucleotide encoding a known transcription factor DNA binding domain polypeptide sequence.

Yeast strains carrying these reporter constructs produce low levels of beta-galactosidase and form white colonies on filters containing X-gal. The reporter strains carrying wild-type transcription factor binding promoter element sequences are transformed with a polynucleotide that encodes a polypeptide comprising a plant transcription factor DNA binding domain operably linked to the acidic activator domain of the yeast GAL4 transcription factor, “GAL4-ACT”. The clones that contain a polynucleotide encoding a transcription factor DNA binding domain operably linked to GLA4-ACT can bind upstream of the lacZ reporter genes carrying the wild-type transcription factor binding promoter element sequence, activate transcription of the lacZ gene and result in yeast forming blue colonies on X-gal-treated filters.

Upon screening about 2×10⁶ yeast transformants, positive cDNA clones are isolated; i.e., clones that cause yeast strains carrying lacZ reporters operably linked to wild-type transcription factor binding promoter elements to form blue colonies on X-gal-treated filters. The cDNA clones do not cause a yeast strain carrying a mutant type transcription factor binding promoter elements fused to LacZ to turn blue. Thus, a polynucleotide encoding transcription factor DNA binding domain, a conserved domain, is shown to activate transcription of a gene.

Example XI Gel Shift Assays

The presence of a transcription factor comprising a DNA binding domain which binds to a DNA transcription factor binding element is evaluated using the following gel shift assay. The transcription factor is recombinantly expressed and isolated from E. coli or isolated from plant material. Total soluble protein, including transcription factor, (40 ng) is incubated at room temperature in 10 μl of 1× binding buffer (15 mM HEPES (pH 7.9), 1 mM EDTA, 30 mM KCl, 5% glycerol, 5% bovine serum albumin, 1 mM DTT) plus 50 ng poly(dl-dC):poly(dl-dC) (Pharmacia, Piscataway N.J.) with or without 100 ng competitor DNA. After 10 minutes incubation, probe DNA comprising a DNA transcription factor binding element (1 ng) that has been ³²P-labeled by end-filling (Sambrook et al. (1989) supra) is added and the mixture incubated for an additional 10 minutes. Samples are loaded onto polyacrylamide gels (4% w/v) and fractionated by electrophoresis at 150V for 2 h (Sambrook et al. supra). The degree of transcription factor-probe DNA binding is visualized using autoradiography. Probes and competitor DNAs are prepared from oligonucleotide inserts ligated into the BamHI site of pUC118 (Vieira et al. (1987) Methods Enzymol. 153: 3-11). Orientation and concatenation number of the inserts are determined by dideoxy DNA sequence analysis (Sambrook et al. supra). Inserts are recovered after restriction digestion with EcoRI and HindIII and fractionation on polyacrylamide gels (12% w/v) (Sambrook et al. supra).

Example XII Introduction of Polynucleotides into Dicotyledonous Plants

Transcription factor sequences listed in the Sequence Listing recombined into pMEN20 or pMEN65 expression vectors are transformed into a plant for the purpose of modifying plant traits. The cloning vector may be introduced into a variety of cereal plants by means well known in the art such as, for example, direct DNA transfer or Agrobacterium tumefaciens-mediated transformation. It is now routine to produce transgenic plants using most dicot plants (see Weissbach and Weissbach, (1989) supra; Gelvin et al. (1990) supra; Herrera-Estrella et al. (1983) supra; Bevan (1984) supra; and Klee (1985) supra). Methods for analysis of traits are routine in the art and examples are disclosed above.

Example XIII Transformation of Cereal Plants with an Expression Vector

Cereal plants such as, but not limited to, corn, wheat, rice, sorghum, or barley, may also be transformed with the present polynucleotide sequences in pMEN20 or pMEN65 expression vectors for the purpose of modifying plant traits. For example, pMEN020 may be modified to replace the NptII coding region with the BAR gene of Streptomyces hygroscopicus that confers resistance to phosphinothricin. The KpnI and BglII sites of the Bar gene are removed by site-directed mutagenesis with silent codon changes.

The cloning vector may be introduced into a variety of cereal plants by means well known in the art such as, for example, direct DNA transfer or Agrobacterium tumefaciens-mediated transformation. It is now routine to produce transgenic plants of most cereal crops (Vasil (1994) Plant Mol. Biol. 25: 925-937) such as corn, wheat, rice, sorghum (Cassas et al. (1993) Proc. Natl. Acad. Sci. 90: 11212-11216, and barley (Wan and Lemeaux (1994) Plant Physiol. 104:37-48. DNA transfer methods such as the microprojectile can be used for corn (Fromm et al. (1990) Bio/Technol. 8: 833-839); Gordon-Kamm et al. (1990) Plant Cell 2: 603-618; Ishida (1990) Nature Biotechnol. 14:745-750), wheat (Vasil et al. (1992) Bio/Technol. 10:667-674; Vasil et al. (1993) Bio/Technol. 11:1553-1558; Weeks et al. (1993) Plant Physiol. 102:1077-1084), rice (Christou (1991) Bio/Technol. 9:957-962; Hiei et al. (1994) Plant J. 6:271-282; Aldemita and Hodges (1996) Planta 199:612-617; and Hiei et al. (1997) Plant Mol. Biol. 35:205-218). For most cereal plants, embryogenic cells derived from immature scutellum tissues are the preferred cellular targets for transformation (Hiei et al. (1997) Plant Mol. Biol. 35:205-218; Vasil (1994) Plant Mol. Biol. 25: 925-937).

Vectors according to the present invention may be transformed into corn embryogenic cells derived from immature scutellar tissue by using microprojectile bombardment, with the A188XB73 genotype as the preferred genotype (Fromm et al. (1990) Bio/Technol. 8: 833-839; Gordon-Kamm et al. (1990) Plant Cell 2: 603-618). After microprojectile bombardment the tissues are selected on phosphinothricin to identify the transgenic embryogenic cells (Gordon-Kamm et al. (1990) Plant Cell 2: 603-618). Transgenic plants are regenerated by standard corn regeneration techniques (Fromm et al. (1990) Bio/Technol. 8: 833-839; Gordon-Kamm et al. (1990) Plant Cell 2: 603-618).

The plasmids prepared as described above can also be used to produce transgenic wheat and rice plants (Christou (1991) Bio/Technol. 9:957-962; Hiei et al. (1994) Plant J. 6:271-282; Aldemita and Hodges (1996) Planta 199:612-617; and Hiei et al. (1997) Plant Mol. Biol. 35:205-218) that coordinately express genes of interest by following standard transformation protocols known to those skilled in the art for rice and wheat (Vasil et al. (1992) Bio/Technol. 10:667-674; Vasil et al. (1993) Bio/Technol. 11:1553-1558; and Weeks et al. (1993) Plant Physiol. 102:1077-1084), where the bar gene is used as the selectable marker.

Example XIV Identification of Orthologous and Paralogous Sequences

Orthologs to Arabidopsis genes may identified by several methods, including hybridization, amplification, or bioinformatically. This example describes how one may identify equivalogs to the Arabidopsis AP2 family transcription factor CBF1 (polynucleotide SEQ ID NO: 1955, encoded polypeptide SEQ ID NO: 1956), which confers tolerance to abiotic stresses (Thomashow et al. (2002) U.S. Pat. No. 6,417,428), and an example to confirm the function of homologous sequences. In this example, orthologs to CBF1 were found in canola (Brassica napus) using polymerase chain reaction (PCR).

Degenerate primers were designed for regions of AP2 binding domain and outside of the AP2 (carboxyl terminal domain):

Mol 368 (reverse) (SEQ ID NO: 2205) 5′-CAY CCN ATH TAY MGN GGN GT-3′ Mol 378 (forward) (SEQ ID NO: 2206) 5′-GGN ARN ARC ATN CCY TCN GCC-3′ (Y: C/T, N: A/C/G/T, H: A/C/T, M: A/C, R: AG)

Primer Mol 368 is in the AP2 binding domain of CBF1 (amino acid sequence: His-Pro-Ile-Tyr-Arg-Gly-Val, SEQ ID NO: 2909) while primer Mol 378 is outside the AP2 domain (carboxyl terminal domain) (amino acid sequence: Met-Ala-Glu-Gly-Met-Leu-Leu-Pro, SEQ ID NO: 2910).

The genomic DNA isolated from B. napus was PCR-amplified by using these primers following these conditions: an initial denaturation step of 2 min at 93° C.; 35 cycles of 93° C. for 1 min, 55° C. for 1 min, and 72° C. for 1 min; and a final incubation of 7 min at 72° C. at the end of cycling.

The PCR products were separated by electrophoresis on a 1.2% agarose gel and transferred to nylon membrane and hybridized with the AT CBF1 probe prepared from Arabidopsis genomic DNA by PCR amplification. The hybridized products were visualized by colorimetric detection system (Boehringer Mannheim) and the corresponding bands from a similar agarose gel were isolated using the Qiagen Extraction Kit (Qiagen). The DNA fragments were ligated into the TA clone vector from TOPO TA Cloning Kit (Invitrogen) and transformed into E. coli strain TOP10 (Invitrogen).

Seven colonies were picked and the inserts were sequenced on an ABI 377 machine from both strands of sense and antisense after plasmid DNA isolation. The DNA sequence was edited by sequencer and aligned with the AtCBF1 by GCG software and NCBI blast searching.

The nucleic acid sequence and amino acid sequence of one canola ortholog found in this manner (bnCBF1; polynucleotide SEQ ID NO: 2203 and polypeptide SEQ ID NO: 2204) identified by this process is shown in the Sequence Listing.

The aligned amino acid sequences show that the bnCBF1 gene has 88% identity with the Arabidopsis sequence in the AP2 domain region and 85% identity with the Arabidopsis sequence outside the AP2 domain when aligned for two insertion sequences that are outside the AP2 domain.

Similarly, paralogous sequences to Arabidopsis genes, such as CBF1, may also be identified.

Two paralogs of CBF1 from Arabidopsis thaliana: CBF2 and CBF3. CBF2 and CBF3 have been cloned and sequenced as described below. The sequences of the DNA SEQ ID NO: 1957 and 1959 and encoded proteins SEQ ID NO: 1958 and 1960 are set forth in the Sequence Listing.

A lambda cDNA library prepared from RNA isolated from Arabidopsis thaliana ecotype Columbia (Lin and Thomashow (1992) Plant Physiol. 99: 519-525) was screened for recombinant clones that carried inserts related to the CBF1 gene (Stockinger et al. (1997) Proc. Natl. Acad. Sci. 94:1035-1040). CBF1 was ³²P-radiolabeled by random priming (Sambrook et al. supra) and used to screen the library by the plaque-lift technique using standard stringent hybridization and wash conditions (Hajela et al. (1990) Plant Physiol. 93:1246-1252; Sambrook et al. supra) 6×SSPE buffer, 60° C. for hybridization and 0.1×SSPE buffer and 60° C. for washes). Twelve positively hybridizing clones were obtained and the DNA sequences of the cDNA inserts were determined. The results indicated that the clones fell into three classes. One class carried inserts corresponding to CBF1. The two other classes carried sequences corresponding to two different homologs of CBF1, designated CBF2 and CBF3. The nucleic acid sequences and predicted protein coding sequences for Arabidopsis CBF1, CBF2 and CBF3 are listed in the Sequence Listing (SEQ ID NOs: 1955, 1957, 1959 and SEQ ID NOs: 1956, 1958, 1960, respectively). The nucleic acid sequences and predicted protein coding sequence for Brassica napus CBF ortholog is listed in the Sequence Listing (SEQ ID NOs: 2203 and 2204, respectively).

A comparison of the nucleic acid sequences of Arabidopsis CBF1, CBF2 and CBF3 indicate that they are 83 to 85% identical as shown in Table 11.

TABLE 11 Percent identity^(a) DNA^(b) Polypeptide cbf1/cbf2 85 86 cbf1/cbf3 83 84 cbf2/cbf3 84 85 ^(a)Percent identity was determined using the Clustal algorithm from the Megalign program (DNASTAR, Inc.). ^(b)Comparisons of the nucleic acid sequences of the open reading frames are shown.

Similarly, the amino acid sequences of the three CBF polypeptides range from 84 to 86% identity. An alignment of the three amino acidic sequences reveals that most of the differences in amino acid sequence occur in the acidic C-terminal half of the polypeptide. This region of CBF1 serves as an activation domain in both yeast and Arabidopsis (not shown).

Residues 47 to 106 of CBF1 correspond to the AP2 domain of the protein, a DNA binding motif that to date, has only been found in plant proteins. A comparison of the AP2 domains of CBF1, CBF2 and CBF3 indicates that there are a few differences in amino acid sequence. These differences in amino acid sequence might have an effect on DNA binding specificity.

Example XV Transformation of Canola with a Plasmid Containing CBF1, CBF2, or CBF3

After identifying homologous genes to CBF1, canola was transformed with a plasmid containing the Arabidopsis CBF1, CBF2, or CBF3 genes cloned into the vector pGA643 (An (1987) Methods Enzymol. 253: 292). In these constructs the CBF genes were expressed constitutively under the CaMV 35S promoter. In addition, the CBF1 gene was cloned under the control of the Arabidopsis COR15 promoter in the same vector pGA643. Each construct was transformed into Agrobacterium strain GV3101. Transformed Agrobacteria were grown for 2 days in minimal AB medium containing appropriate antibiotics.

Spring canola (B. napus cv. Westar) was transformed using the protocol of Moloney et al. (1989) Plant Cell Reports 8: 238, with some modifications as described. Briefly, seeds were sterilized and plated on half strength MS medium, containing 1% sucrose. Plates were incubated at 24° C. under 60-80 μE/m²s light using a 16 hour light/8 hour dark photoperiod. Cotyledons from 4-5 day old seedlings were collected, the petioles cut and dipped into the Agrobacterium solution. The dipped cotyledons were placed on co-cultivation medium at a density of 20 cotyledons/plate and incubated as described above for 3 days. Explants were transferred to the same media, but containing 300 mg/l timentin (SmithKline Beecham, Pa.) and thinned to 10 cotyledons/plate. After 7 days explants were transferred to Selection/Regeneration medium. Transfers were continued every 2-3 weeks (2 or 3 times) until shoots had developed. Shoots were transferred to Shoot-Elongation medium every 2-3 weeks. Healthy looking shoots were transferred to rooting medium. Once good roots had developed, the plants were placed into moist potting soil.

The transformed plants were then analyzed for the presence of the NPTII gene/kanamycin resistance by ELISA, using the ELISA NPTII kit from 5Prime-3Prime Inc. (Boulder, Colo.). Approximately 70% of the screened plants were NPTII positive. Only those plants were further analyzed.

From Northern blot analysis of the plants that were transformed with the constitutively expressing constructs, showed expression of the CBF genes and all CBF genes were capable of inducing the Brassica napus cold-regulated gene BN115 (homolog of the Arabidopsis COR15 gene). Most of the transgenic plants appear to exhibit a normal growth phenotype. As expected, the transgenic plants are more freezing tolerant than the wild-type plants. Using the electrolyte leakage of leaves test, the control showed a 50% leakage at −2 to −3° C. Spring canola transformed with either CBF1 or CBF2 showed a 50% leakage at −6 to −7° C. Spring canola transformed with CBF3 shows a 50% leakage at about −10 to −15° C. Winter canola transformed with CBF3 may show a 50% leakage at about −16 to −20° C. Furthermore, if the spring or winter canola are cold acclimated the transformed plants may exhibit a further increase in freezing tolerance of at least −2° C.

To test salinity tolerance of the transformed plants, plants were watered with 150 mM NaCl. Plants overexpressing CBF1, CBF2 or CBF3 grew better compared with plants that had not been transformed with CBF1, CBF2 or CBF3.

These results demonstrate that equivalogs of Arabidopsis transcription factors can be identified and shown to confer similar functions in non-Arabidopsis plant species.

Example XVI Cloning of Transcription Factor Promoters

Promoters are isolated from transcription factor genes that have gene expression patterns useful for a range of applications, as determined by methods well known in the art (including transcript profile analysis with cDNA or oligonucleotide microarrays, Northern blot analysis, semi-quantitative or quantitative RT-PCR). Interesting gene expression profiles are revealed by determining transcript abundance for a selected transcription factor gene after exposure of plants to a range of different experimental conditions, and in a range of different tissue or organ types, or developmental stages. Experimental conditions to which plants are exposed for this purpose includes cold, heat, drought, osmotic challenge, varied hormone concentrations (ABA, GA, auxin, cytokinin, salicylic acid, brassinosteroid), pathogen and pest challenge. The tissue types and developmental stages include stem, root, flower, rosette leaves, cauline leaves, siliques, germinating seed, and meristematic tissue. The set of expression levels provides a pattern that is determined by the regulatory elements of the gene promoter.

Transcription factor promoters for the genes disclosed herein are obtained by cloning 1.5 kb to 2.0 kb of genomic sequence immediately upstream of the translation start codon for the coding sequence of the encoded transcription factor protein. This region includes the 5′-UTR of the transcription factor gene, which can comprise regulatory elements. The 1.5 kb to 2.0 kb region is cloned through PCR methods, using primers that include one in the 3′ direction located at the translation start codon (including appropriate adaptor sequence), and one in the 5′ direction located from 1.5 kb to 2.0 kb upstream of the translation start codon (including appropriate adaptor sequence). The desired fragments are PCR-amplified from Arabidopsis Col-0 genomic DNA using high-fidelity Taq DNA polymerase to minimize the incorporation of point mutation(s). The cloning primers incorporate two rare restriction sites, such as Not1 and Sfi1, found at low frequency throughout the Arabidopsis genome. Additional restriction sites are used in the instances where a Not1 or Sfi1 restriction site is present within the promoter.

The 1.5-2.0 kb fragment upstream from the translation start codon, including the 5′-untranslated region of the transcription factor, is cloned in a binary transformation vector immediately upstream of a suitable reporter gene, or a transactivator gene that is capable of programming expression of a reporter gene in a second gene construct. Reporter genes used include green fluorescent protein (and related fluorescent protein color variants), beta-glucuronidase, and luciferase. Suitable transactivator genes include LexA-GAL4, along with a transactivatable reporter in a second binary plasmid (as disclosed in U.S. patent application Ser. No. 09/958,131, incorporated herein by reference). The binary plasmid(s) is transferred into Agrobacterium and the structure of the plasmid confirmed by PCR. These strains are introduced into Arabidopsis plants as described in other examples, and gene expression patterns determined according to standard methods know to one skilled in the art for monitoring GFP fluorescence, beta-glucuronidase activity, or luminescence.

All references, publications, patent documents, web pages, and other documents cited or mentioned herein are hereby incorporated by reference in their entirety for all purposes. Although the invention has been described with reference to specific embodiments and examples, it should be understood that one of ordinary skill can make various modifications without departing from the spirit of the invention. The scope of the invention is not limited to the specific embodiments and examples provided. 

1. A transgenic plant comprising a recombinant polynucleotide encoding a polypeptide that comprises a conserved domain that has at least 95% identity to amino acids 240-297 of SEQ ID NO: 28; wherein, due to expression of the polypeptide in the transgenic plant, the transgenic plant has greater yield with respect to a control plant that has not been transformed with the recombinant polynucleotide.
 2. The transgenic plant of claim 1, wherein expression of the polypeptide is regulated by a tissue-specific, inducible, or constitutive promoter.
 3. The transgenic plant of claim 1, wherein the recombinant polynucleotide comprises SEQ ID NO:
 27. 4. A transformed seed produced by the transgenic plant of claim 1, wherein the transformed seed comprises the recombinant polynucleotide.
 5. A method for producing and selecting a transgenic plant having greater yield, with respect to a control plant, the method including: (a) transforming a target plant with a recombinant polynucleotide that encodes a polypeptide that comprises a conserved domain that has at least 95% identity to amino acids 240-297 of SEQ ID NO: 28; wherein, due to expression of the polypeptide in the transgenic plant, the transgenic plant has greater yield, with respect to a control plant that has not been transformed with the recombinant polynucleotide; and (b) selecting a transgenic plant that has greater yield with respect to the control plant, wherein the control plant has not been transformed with the recombinant polynucleotide.
 6. The method of claim 5, wherein expression of the polypeptide is regulated by a tissue-specific, inducible, or constitutive promoter.
 7. The method of claim 5, wherein the method includes the additional steps of: (c) crossing the transgenic plant with itself or another plant; (d) selecting seed that develops as a result of said crossing; and (e) growing a progeny plant from the seed, wherein the seed comprises the recombinant polynucleotide.
 8. A transgenic plant comprising a recombinant polynucleotide encoding a polypeptide that shares at least 90% amino acid sequence identity with SEQ ID NO: 28; wherein, due to expression of the polypeptide in the transgenic plant, the transgenic plant has greater leaf size, greater biomass, or greater yield with respect to a control plant that has not been transformed with the recombinant polynucleotide.
 9. The transgenic plant of claim 8, wherein expression of the polypeptide is regulated by a tissue-specific, inducible, or constitutive promoter.
 10. A transformed seed produced by the transgenic plant of claim 8, wherein the transformed seed comprises the recombinant polynucleotide.
 11. The transgenic plant of claim 8, wherein the recombinant polynucleotide comprises SEQ ID NO:
 27. 12. The transgenic plant of claim 8, wherein the polypeptide is SEQ ID NO:
 28. 13. A plant cell derived from the transgenic plant of claim 8, wherein the plant cell comprises the recombinant polynucleotide.
 14. Plant tissue or plant material derived from the transgenic plant of claim 8, wherein the plant tissue or plant material comprises the recombinant polynucleotide.
 15. The transgenic plant of claim 8, wherein the transgenic plant has increased leaf size as compared to a control plant that has not been transformed with the recombinant polynucleotide. 